CN210528953U - Kit for biological cell culture - Google Patents
Kit for biological cell culture Download PDFInfo
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- CN210528953U CN210528953U CN201920902155.2U CN201920902155U CN210528953U CN 210528953 U CN210528953 U CN 210528953U CN 201920902155 U CN201920902155 U CN 201920902155U CN 210528953 U CN210528953 U CN 210528953U
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Abstract
The utility model discloses a kit for biological cell culture, including the base, be provided with the fixing base in the base, the fixing base is embedded to have driving motor, and driving motor's output shaft is on the culture dish, and the upper surface of base is provided with dustproof apron, and dustproof apron is provided with reagent on the dustproof apron through cutting fixed mounting on the base, and reagent adds the mechanism including dropwise add pipe, tee bend, first drainage tube, second drainage tube and limiting plate. The utility model discloses kit for biological cell culture, at the in-process of adding reagent, need not take off dustproof apron to can avoid external environment to pollute the culture dish, and then can improve the quality of cultivateing the cell, can prevent through dustproof sealing lid that debris such as dust from getting into the dropwise add intraductal and pollute reagent interpolation mechanism, can be convenient for take dustproof sealing lid through connecting the rope, avoid dustproof sealing lid because random the locating and can't look for and then increase operating personnel's the time of looking for, make cell culture inefficiency.
Description
Technical Field
The utility model relates to a cell culture technical field specifically is a biological kit for cell culture.
Background
Cell culture (cell culture) refers to a method for simulating in vivo environment (sterility, suitable temperature, pH value, certain nutritional conditions, and the like) in vitro to enable the cells to survive, grow, reproduce and maintain main structures and functions, and is also called cell cloning technology, and the formal term in biology is cell culture technology.
When carrying out biological cell culture, need add a quantitative reagent to in the culture dish, prior art's kit for biological cell culture is when adding the reagent, mostly takes off dustproof apron, and the manual work carries out the interpolation of reagent, adds the in-process at the reagent, owing to taken off dustproof apron, makes the culture dish be arranged in an open environment, makes the culture dish very easily receive the pollution, leads to the quality of cultured cell poor, consequently, the above-mentioned problem is solved to the urgent need for a kit for biological cell culture.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a kit for biological cell culture, at the in-process of adding reagent, need not take off dustproof cover plate, thereby can avoid external environment pollution culture dish, and then can improve the quality of cultivateing the cell, can prevent through dustproof sealing lid that debris such as dust from getting into the dropwise add intraductal and pollute reagent interpolation mechanism, dustproof sealing lid of can being convenient for take through connecting the rope, avoid dustproof sealing lid because can't find and then increase operating personnel's the time of looking for everywhere in disorder, make cell culture inefficiency, the problem among the prior art has been solved.
In order to achieve the above object, the utility model provides a following technical scheme: a kit for biological cell culture comprises a base, wherein a fixed seat is arranged on the inner wall of the bottom of the base, a driving motor is embedded in the fixed seat, an output shaft of the driving motor penetrates through the fixed seat and extends to the outer side of the fixed seat to be connected onto a culture dish, guide pillars are arranged on the lower surface of the culture dish and are distributed at equal intervals, and one end, far away from the culture dish, of each guide pillar is installed on the fixed seat;
the upper surface of the base is provided with a dustproof cover plate, the lower surface of the dustproof cover plate is provided with symmetrically distributed inserting strips, the dustproof cover plate is fixedly arranged on the base through the inserting strips, and a reagent adding mechanism is arranged on the dustproof cover plate;
the reagent adding mechanism comprises a dropping pipe, a tee joint, a first drainage pipe, a second drainage pipe and a limiting plate, the limiting plates are arranged on the peripheries of the first drainage pipe and the second drainage pipe, the first drainage pipe and the second drainage pipe are arranged on the dustproof cover plate through the limiting plates, and the first drainage pipe and the second drainage pipe are connected with the dropping pipe through the tee joint;
be provided with dustproof sealing mechanism on the dropwise add pipe, dustproof sealing mechanism includes dustproof sealing lid, connects rope and link, the both ends of connecting the rope all set up the link, the one end of connecting the rope is passed through the link and is installed on the dropwise add pipe, the other end of connecting the rope is connected with dustproof sealing lid through the link, dustproof sealing lid covers on the dropwise add pipe.
Preferably, the culture dish is a circular structure member.
Preferably, the culture dish comprises an inner culture area and an outer culture area, wherein the outer culture area is positioned at the periphery of the inner culture area and is concentric with the inner culture area.
Preferably, all be provided with the baffle on inlayer cultivation district and the outer cultivation district, inlayer cultivation district and outer cultivation district are all divided into not less than five culture tanks through the baffle.
Preferably, the fixed seat is provided with a sliding groove for the guide pillar to move.
Compared with the prior art, the beneficial effects of the utility model are as follows:
1. the utility model discloses kit for biological cell culture, driving motor drive culture dish is rotatory, meanwhile reagent passes through in the dropwise add pipe gets into the tee bend and shunts to first drainage tube and second drainage tube, but add reagent through the culture dish of first drainage tube and second drainage tube pairing rotation, the whole reagent that has added of culture tank that sets up on the culture dish, it is rotatory through driving motor drive culture dish, make reagent interpolation mechanism can carry out reagent interpolation to the culture dish, at the in-process of adding reagent, need not take off dustproof cover plate, thereby can avoid external environment to pollute the culture dish, and then can improve the quality of cultured cell.
2. The utility model discloses kit for biological cell culture, after the reagent adds, with the dustproof sealing lid on the dropwise add pipe, can prevent through the dustproof sealing lid that debris such as dust from getting into the dropwise add intraductal and pollute reagent interpolation mechanism, can be convenient for take the dustproof sealing lid through connecting the rope, avoid the dustproof sealing lid because put in disorder everywhere and can't find and then increase operating personnel's the time of looking for, make cell culture inefficiency.
Drawings
FIG. 1 is a schematic view of a biological cell culture kit according to the present invention;
FIG. 2 is a top view of the culture dish of the present invention;
FIG. 3 is a top view of the inner culture zone of the present invention;
FIG. 4 is a top view of the sliding groove formed on the fixing base of the present invention;
FIG. 5 is an enlarged view of the point A of the present invention;
FIG. 6 is an enlarged view of the reagent adding mechanism of the present invention;
fig. 7 is an enlarged view of the dust seal mechanism of the present invention.
In the figure: 1. a base; 2. a fixed seat; 21. a drive motor; 22. a chute; 3. a culture dish; 31. an inner culture zone; 311. a culture tank; 32. an outer culture zone; 4. a guide post; 5. a dust-proof cover plate; 51. cutting; 6. a reagent addition mechanism; 61. a dropping pipe; 62. a tee joint; 63. a first draft tube; 64. a second draft tube; 65. a limiting plate; 7. a dust sealing mechanism; 71. a dust-proof sealing cover; 72. connecting ropes; 73. hanging a ring; 8. a separator.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-4, a kit for biological cell culture, includes a base 1, a fixing base 2 is disposed on an inner wall of a bottom of the base 1, a driving motor 21 is embedded in the fixing base 2, an output shaft of the driving motor 21 extends to an outer side of the fixing base 2 through the fixing base 2 and is connected to a culture dish 3, the culture dish 3 is a circular structural member, the culture dish 3 includes an inner culture area 31 and an outer culture area 32, the outer culture area 32 is located at a periphery of the inner culture area 31 and is concentric with the inner culture area 31, the inner culture area 31 and the outer culture area 32 are both provided with a partition plate 8, the inner culture area 31 and the outer culture area 32 are both divided into at least five culture tanks 311 by the partition plate 8, the driving motor 21 drives the culture dish 3 to rotate on the fixing base 2 under a supporting and guiding effect of a guide post 4, while the culture dish 3 rotates, a reagent is added into a dropping pipe 61, reagent passes through in dropping pipe 61 gets into tee bend 62 and shunts to first drainage tube 63 and second drainage tube 64, but culture dish 3 interpolation reagent of pairing rotation through first drainage tube 63 and second drainage tube 64, the whole reagent that has added of culture tank 311 that sets up on culture dish 3, at the in-process of adding reagent, need not take off dustproof cover plate 5, thereby can avoid external environment pollution culture dish 3, and then can improve the quality of cultured cell, the lower surface of culture dish 3 is provided with equidistant distribution's guide pillar 4, the one end that culture dish 3 was kept away from to guide pillar 4 is installed on fixing base 2, be provided with the spout 22 that supplies guide pillar 4 motion on fixing base 2, driving motor 21 drive culture dish 3 is rotatory on fixing base 2 under the support guide effect of guide pillar 4.
Referring to fig. 5, a dustproof cover plate 5 is disposed on the upper surface of the base 1, inserting strips 51 are symmetrically disposed on the lower surface of the dustproof cover plate 5, the dustproof cover plate 5 is fixedly mounted on the base 1 through the inserting strips 51, so that the dustproof cover plate 5 is convenient to mount or dismount, the dustproof cover plate 5 can prevent the external environment from polluting the culture dish 3, the quality of cultured cells can be improved, and a reagent adding mechanism 6 is disposed on the dustproof cover plate 5.
Referring to fig. 6, the reagent adding mechanism 6 includes a dropping pipe 61, a tee 62, a first drainage pipe 63, a second drainage pipe 64 and a limiting plate 65, the limiting plate 65 is disposed at the periphery of the first drainage pipe 63 and the second drainage pipe 64, the first drainage pipe 63 and the second drainage pipe 64 are mounted on the dustproof cover plate 5 through the limiting plate 65, the first drainage pipe 63 and the second drainage pipe 64 are connected with the dropping pipe 61 through the tee 62, reagent is added into the dropping pipe 61, the reagent enters the tee 62 through the dropping pipe 61 and is distributed into the first drainage pipe 63 and the second drainage pipe 64, and the reagent can be added to the rotating culture dish 3 through the first drainage pipe 63 and the second drainage pipe 64.
Referring to fig. 7, a dustproof sealing mechanism 7 is arranged on the dropping pipe 61, the dustproof sealing mechanism 7 includes a dustproof sealing cover 71, a connecting rope 72 and a hanging ring 73, both ends of the connecting rope 72 are provided with the hanging ring 73, one end of the connecting rope 72 is installed on the dropping pipe 61 through the hanging ring 73, the other end of the connecting rope 72 is connected with the dustproof sealing cover 71 through the hanging ring 73, the dustproof sealing cover 71 covers the dropping pipe 61, after the reagent is added, the dustproof sealing cover 71 covers the dropping pipe 61, the dustproof sealing cover 71 can prevent dust and other impurities from entering the dropping pipe 61 to pollute the reagent adding mechanism 6, the dustproof sealing cover 71 can be conveniently taken through the connecting rope 72, the dustproof sealing cover 71 is prevented from being randomly placed everywhere and being unable to find, so that the finding time of operators is increased, and the cell culture efficiency is low.
The working process is as follows: when reagent needs to be added into the culture dish 3, the driving motor 21 drives the culture dish 3 to rotate on the fixing seat 2 under the supporting and guiding effect of the guide pillar 4, when the culture dish 3 rotates, reagent is added into the dropping pipe 61, the reagent enters the tee joint 62 through the dropping pipe 61 and is shunted to the first drainage tube 63 and the second drainage tube 64, the reagent can be added into the culture dish 3 to rotate through the first drainage tube 63 and the second drainage tube 64, the reagent is completely added to the culture groove 311 arranged on the culture dish 3, the culture dish 3 is driven to rotate through the driving motor 21, the reagent adding mechanism 6 can add the reagent into the culture dish 3, in the reagent adding process, the dustproof cover plate 5 does not need to be taken down, the culture dish 3 can be prevented from being polluted by the external environment, and the quality of cultured cells can be improved.
In summary, the following steps: the utility model discloses a kit for biological cell culture, when needing to add reagent to in the culture dish 3, driving motor 21 drive culture dish 3 rotates on fixing base 2 under the support guiding action of guide pillar 4, when culture dish 3 is rotatory, add reagent to in dropping tube 61, reagent gets into tee bend 62 and shunts to first drainage tube 63 and second drainage tube 64 through dropping tube 61, can add reagent to culture dish 3 of pairing rotation through first drainage tube 63 and second drainage tube 64, until culture tank 311 that sets up on culture dish 3 has all added reagent, drive culture dish 3 through driving motor 21 and rotate, make reagent adding mechanism 6 can carry out reagent addition to culture dish 3, in the process of adding reagent, need not take off dust cover 5, thereby can avoid external environment pollution culture dish 3, and then can improve the quality of cultured cells, after reagent addition is finished, cover dustproof sealing lid 71 on dropwise add pipe 61, can prevent through dustproof sealing lid 71 that debris such as dust from getting into dropwise add pipe 61 in and polluting reagent and add mechanism 6, can be convenient for take dustproof sealing lid 71 through connecting rope 72, avoid dustproof sealing lid 71 because the random of everywhere can not find and then increase operating personnel's the time of looking for, make cell culture inefficiency.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (5)
1. A kit for biological cell culture, comprising a base (1), characterized in that: the inner wall of the bottom of the base (1) is provided with a fixed seat (2), a driving motor (21) is embedded in the fixed seat (2), an output shaft of the driving motor (21) penetrates through the fixed seat (2) and extends to the outer side of the fixed seat (2) to be connected onto a culture dish (3), guide pillars (4) distributed at equal intervals are arranged on the lower surface of the culture dish (3), and one end, far away from the culture dish (3), of each guide pillar (4) is installed on the fixed seat (2);
the reagent adding device is characterized in that a dustproof cover plate (5) is arranged on the upper surface of the base (1), inserting strips (51) which are symmetrically distributed are arranged on the lower surface of the dustproof cover plate (5), the dustproof cover plate (5) is fixedly arranged on the base (1) through the inserting strips (51), and a reagent adding mechanism (6) is arranged on the dustproof cover plate (5);
the reagent adding mechanism (6) comprises a dropping pipe (61), a tee joint (62), a first drainage pipe (63), a second drainage pipe (64) and limiting plates (65), the limiting plates (65) are arranged on the peripheries of the first drainage pipe (63) and the second drainage pipe (64), the first drainage pipe (63) and the second drainage pipe (64) are installed on the dustproof cover plate (5) through the limiting plates (65), and the first drainage pipe (63) and the second drainage pipe (64) are connected with the dropping pipe (61) through the tee joint (62);
be provided with dustproof sealing mechanism (7) on drip tube (61), dustproof sealing mechanism (7) are including dustproof sealing lid (71), are connected rope (72) and link (73), the both ends of connecting rope (72) all set up link (73), the one end of connecting rope (72) is passed through link (73) and is installed on drip tube (61), the other end of connecting rope (72) is connected with dustproof sealing lid (71) through link (73), dustproof sealing lid (71) lid is on drip tube (61).
2. The biological cell culture kit according to claim 1, wherein: the culture dish (3) is a circular structural component.
3. The biological cell culture kit according to claim 1, wherein: the culture dish (3) comprises an inner culture area (31) and an outer culture area (32), wherein the outer culture area (32) is positioned on the periphery of the inner culture area (31) and is concentric with the inner culture area (31).
4. The biological cell culture kit according to claim 3, wherein: all be provided with baffle (8) on inlayer cultivation district (31) and outer cultivation district (32), inlayer cultivation district (31) and outer cultivation district (32) all divide into culture tank (311) that are no less than five through baffle (8).
5. The biological cell culture kit according to claim 1, wherein: and a sliding groove (22) for the guide post (4) to move is arranged on the fixed seat (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920902155.2U CN210528953U (en) | 2019-06-14 | 2019-06-14 | Kit for biological cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920902155.2U CN210528953U (en) | 2019-06-14 | 2019-06-14 | Kit for biological cell culture |
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| Publication Number | Publication Date |
|---|---|
| CN210528953U true CN210528953U (en) | 2020-05-15 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201920902155.2U Active CN210528953U (en) | 2019-06-14 | 2019-06-14 | Kit for biological cell culture |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111808923A (en) * | 2020-07-20 | 2020-10-23 | 四川大学华西医院 | Gene detection method and device |
| CN113150982A (en) * | 2021-02-04 | 2021-07-23 | 张仪 | Novel cell biotechnology, kit and preparation device |
-
2019
- 2019-06-14 CN CN201920902155.2U patent/CN210528953U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111808923A (en) * | 2020-07-20 | 2020-10-23 | 四川大学华西医院 | Gene detection method and device |
| CN111808923B (en) * | 2020-07-20 | 2024-03-05 | 四川大学华西医院 | Gene detection method and device |
| CN113150982A (en) * | 2021-02-04 | 2021-07-23 | 张仪 | Novel cell biotechnology, kit and preparation device |
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