CN211241208U - A device for alfalfa haploid is cultivateed - Google Patents
A device for alfalfa haploid is cultivateed Download PDFInfo
- Publication number
- CN211241208U CN211241208U CN201922372246.2U CN201922372246U CN211241208U CN 211241208 U CN211241208 U CN 211241208U CN 201922372246 U CN201922372246 U CN 201922372246U CN 211241208 U CN211241208 U CN 211241208U
- Authority
- CN
- China
- Prior art keywords
- filter screen
- upper cover
- rack
- rotating shaft
- alfalfa
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a device for culturing alfalfa haploid, which comprises a motor, a bracket, a centrifugal cylinder, an upper cover, a rotating shaft, a placing rack, a filter screen and a sealing cover, wherein the centrifugal cylinder is fixedly arranged on the bracket; the upper cover is detachably arranged at the upper opening of the centrifugal cylinder, and the top surface of the upper cover is provided with an installation seat; the motor is reversely and detachably arranged in the mounting seat; the rotating shaft is rotatably arranged at the bottom of the upper cover, and the top end of the rotating shaft penetrates through the upper cover to be in transmission connection with an output shaft of the motor; the top surface of the sealing cover is fixedly connected with the bottom end of the rotating shaft; the filter screen expand and place on the rack to the suitable for reading edge setting of filter screen is at the rack up end, and closing cap detachable installs the up end at the rack, with pressing from both sides tight filter screen, the utility model discloses, simple structure, convenient to use.
Description
Technical Field
The utility model relates to a technical field of agricultural production equipment specifically is a device that is used for alfalfa haploid to cultivate.
Background
Haploid breeding has huge commercial application value, haploid plants are generated by utilizing haploid induction, and then natural or artificial doubling is carried out, so that homozygous breeding materials can be obtained within two years, the selection process can be remarkably accelerated, and the breeding period can be shortened. Alfalfa (Medicago sativa L.) belongs to perennial cross-pollinated plants, the genetic composition of the group is complex and diverse, the genotype is in a heterozygous state for a long time, and the plants are incompatible by self-crossing, so the alfalfa mainly adopts methods of mixed selection, comprehensive variety breeding, recurrent selection and the like to breed new varieties. However, the method at least needs 6-7 years to breed an excellent group, homozygous materials are extremely difficult to obtain, the breeding period is long, the breeding difficulty is high, the efficiency is low, haploid breeding is one of ideal choices of alfalfa breeding ways, the materials with completely homozygous genotypes can be bred only through two generations, the breeding process is simplified, and the breeding efficiency is improved.
At present, the culture technology of plant free microspores becomes one of the most important means for obtaining haploid plants. Research on microspore culture technology is carried out on more than 250 species, the embryogenic rate of alfalfa cultured by adopting free microspores is low, and no report on obtaining haploid plants exists. Therefore, the method has an urgent need to improve the embryogenic rate of the alfalfa microspores by optimizing factors influencing the embryogenic rate of the plant microspores, so as to obtain haploid plants, and has important theoretical significance and practical value for accelerating the application of the microspore culture technology in alfalfa breeding.
In the alfalfa dissociation microspore culture technology, a mixed culture medium solution is prepared by an MS liquid culture medium and a B5 liquid culture medium according to the volume ratio of 1:1, microspores are dissociated in the mixed culture medium solution by slight squeezing of a pestle, filtrate is collected by filtering of a filter screen, centrifugation is carried out for 3 times, 3min is carried out each time, then the filtrate is suspended in a mixed culture medium consisting of half amount of the MS liquid culture medium and half amount of NLN liquid culture medium, and special centrifugal equipment is used in the step.
Disclosure of Invention
An object of the utility model is to provide a device for alfalfa haploid cultivates for centrifugation mixed culture medium solution.
In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a device for alfalfa haploid is cultivateed, includes motor, support, centrifuge tube, upper cover, rotation axis, rack, filter screen and closing cap, wherein:
the centrifugal cylinder is fixedly arranged on the bracket;
the upper cover is detachably arranged at the upper opening of the centrifugal cylinder, and the top surface of the upper cover is provided with an installation seat;
the motor is reversely and detachably arranged in the mounting seat;
the rotating shaft is rotatably arranged at the bottom of the upper cover, and the top end of the rotating shaft penetrates through the upper cover to be in transmission connection with an output shaft of the motor;
the top surface of the sealing cover is fixedly connected with the bottom end of the rotating shaft;
the filter screen expand and place on the rack to the edge setting of the suitable for reading of filter screen is at the rack up end, and the closing cap detachable installs the up end at the rack, in order to press from both sides tight filter screen.
Further, the filter screen is a 400-mesh aperture nylon gauze.
Further, the rack include annular seat and U-shaped bracing piece, the U-shaped bracing piece set up many, many U-shaped bracing pieces set up in annular seat bottom to the bearing filter screen.
Compared with the prior art, the beneficial effects of the utility model are that:
simple structure, convenient to use, the motor drives the rack rotation, and mixed culture medium solution is located the filter screen, under the centrifugation, filters out in liquid enters into the centrifuge bowl, convenient to use.
Drawings
Fig. 1 is a schematic cross-sectional view of the present invention.
Wherein: the centrifugal machine comprises a motor-1, a support-2, a centrifugal cylinder-3, an upper cover-4, a rotating shaft-5, a placing rack-6, a filter screen-7, a sealing cover-8, a mounting seat-9, an annular structure-10, a bearing seat-11, an annular seat-12 and a U-shaped supporting rod-13.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to the attached drawings, the device for alfalfa haploid cultivation comprises a motor 1, a bracket 2, a centrifuge bowl 3, an upper cover 4, a rotating shaft 5, a placing rack 6, a filter screen 7 and a sealing cover 8, wherein:
centrifuge bowl 3 fixed setting on support 2, centrifuge bowl 3 is stainless steel, and the radius of the inner chamber bottom of centrifuge bowl 3 is convenient for concentrate the filtrating.
The upper cover 4 is detachably arranged at the upper opening of the centrifugal cylinder 3, the top surface of the upper cover 4 is provided with a mounting seat 9, the structure of the mounting seat 9 is set according to the actual situation, and the stability of the motor 1 must be ensured.
The motor 1 is inversely and detachably mounted in the mounting seat 9, in this embodiment, an annular fixing structure 10 is arranged on one side of the motor 1, and the annular fixing structure 10 is connected with the upper end face of the mounting seat 9 by using a screw.
The rotating shaft 5 is rotatably arranged at the bottom of the upper cover 4, the top end of the rotating shaft penetrates through the upper cover 4 to be in transmission connection with an output shaft of the motor 1, a bearing seat 11 is arranged on the bottom surface of the upper cover 4, the rotating shaft 5 is rotatably arranged in the bearing seat 11, the rotating shaft 5 can be stabilized by the bearing seat 11, and the top end of the rotating shaft 5 is connected with the output shaft of the motor 1 through a coupler.
The top surface of the sealing cover 8 is fixedly connected with the bottom end of the rotating shaft 5.
Filter screen 7 expand and place on rack 6 to the edge setting of the suitable for reading of filter screen is at the rack 6 up end, 8 detachable installs at the up end of rack 6 of closing cap to press from both sides tight filter screen, rack 6 include annular seat 12 and U-shaped bracing piece 13, U-shaped bracing piece 13 set up many, many U-shaped bracing pieces 13 set up in annular seat 12 bottoms, with the bearing filter screen, the filter screen is 400 mesh aperture nylon gauze.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (3)
1. The utility model provides a device for alfalfa haploid is cultivateed which characterized in that: including motor, support, centrifuge bowl, upper cover, rotation axis, rack, filter screen and closing cap, wherein:
the centrifugal cylinder is fixedly arranged on the bracket;
the upper cover is detachably arranged at the upper opening of the centrifugal cylinder, and the top surface of the upper cover is provided with an installation seat;
the motor is reversely and detachably arranged in the mounting seat;
the rotating shaft is rotatably arranged at the bottom of the upper cover, and the top end of the rotating shaft penetrates through the upper cover to be in transmission connection with an output shaft of the motor;
the top surface of the sealing cover is fixedly connected with the bottom end of the rotating shaft;
the filter screen expand and place on the rack to the edge setting of the suitable for reading of filter screen is at the rack up end, and the closing cap detachable installs the up end at the rack, in order to press from both sides tight filter screen.
2. The device for alfalfa haploid cultivation as claimed in claim 1, wherein: the filter screen is a 400-mesh aperture nylon gauze.
3. The device for alfalfa haploid cultivation as claimed in claim 2, wherein: the rack include annular seat and U-shaped bracing piece, the U-shaped bracing piece set up many, many U-shaped bracing pieces set up in annular seat bottom to the bearing filter screen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922372246.2U CN211241208U (en) | 2019-12-26 | 2019-12-26 | A device for alfalfa haploid is cultivateed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922372246.2U CN211241208U (en) | 2019-12-26 | 2019-12-26 | A device for alfalfa haploid is cultivateed |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211241208U true CN211241208U (en) | 2020-08-14 |
Family
ID=71963929
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201922372246.2U Expired - Fee Related CN211241208U (en) | 2019-12-26 | 2019-12-26 | A device for alfalfa haploid is cultivateed |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211241208U (en) |
-
2019
- 2019-12-26 CN CN201922372246.2U patent/CN211241208U/en not_active Expired - Fee Related
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN211241208U (en) | A device for alfalfa haploid is cultivateed | |
| CN210796453U (en) | Marsh gas fermentation device beneficial to slag discharge | |
| CN207308554U (en) | A kind of device that organic fertilizer is prepared using breeding waste | |
| CN211267852U (en) | Forestry land trees breeding device | |
| CN216459930U (en) | Seed sieving mechanism is used in agricultural technology popularization | |
| CN211576592U (en) | A sampling device for root system secretion is collected | |
| CN215462587U (en) | Efficient cell filter for preparation of passage cells | |
| CN209997927U (en) | organic fertilizer screening device convenient for taking out impurities | |
| CN219248818U (en) | Be applied to planting case of orchid cultivation | |
| CN218321420U (en) | Microbial agent fermenting installation | |
| CN214344840U (en) | Solid-liquid separation device | |
| CN215627902U (en) | Efficient chlorella-like culture device | |
| CN109041895A (en) | A kind of rotatable flower cultivation culturing frame | |
| CN220915865U (en) | Armpit algae planting pond capable of treating residual vegetable and residual fruit biogas slurry | |
| CN215454533U (en) | Culture apparatus convenient to collect plant roots secretion | |
| CN213652490U (en) | Soil microorganism collection system | |
| CN216931061U (en) | A culture apparatus for forestry is grown seedlings and is used | |
| CN213771859U (en) | Device for manufacturing culture medium by using straw fermentation medium | |
| CN210528701U (en) | Gas production and storage integrated anaerobic digestion device | |
| CN220239292U (en) | Air screen type seed cleaner | |
| CN211912897U (en) | Filtering component for organic fertilizer preparation process | |
| CN220694162U (en) | Water recycling device based on fish and vegetable symbiotic technology | |
| CN211921525U (en) | Rhizobium enrichment culture device | |
| CN213939152U (en) | Rice is cultivated with trading water equipment | |
| CN217594407U (en) | Raw material adding equipment for processing chemical reagent stabilizer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200814 Termination date: 20201226 |