CN210314290U - Ultrasonic crushing's ice bath device - Google Patents
Ultrasonic crushing's ice bath device Download PDFInfo
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- CN210314290U CN210314290U CN201921085731.5U CN201921085731U CN210314290U CN 210314290 U CN210314290 U CN 210314290U CN 201921085731 U CN201921085731 U CN 201921085731U CN 210314290 U CN210314290 U CN 210314290U
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- ice bath
- ice
- container
- storage inner
- ice storage
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Abstract
The utility model discloses a device is bathed to ultrasonic crushing's ice, including ice bath mechanism, ice bath mechanism includes ice bath container and ice storage inner bag, and the ice storage inner bag inlays and installs in ice bath container, and ice storage inner bag top is provided with the recess, is provided with the centrifuging tube in the recess. The utility model discloses simple structure, convenient to use not only can keep low temperature for a long time, has improved cooling efficiency, is favorable to improving crushing effect moreover.
Description
Technical Field
The utility model relates to a biological medicine equipment technical field, more specifically the ice bath device that relates to an ultrasonic crushing that says so.
Background
In the scientific research process of biological medicine, various prokaryotic and eukaryotic expression systems are established by a DNA recombination technology, and the obtained recombinant protein is widely applied to the fields of medical scientific research, disease diagnosis, treatment and other biological medicines. Most of the important target genes are expressed in Escherichia coli, and the target proteins are obtained by crushing cells in the process of obtaining recombinant proteins, and the ultrasonication method is generally adopted in laboratories. In order to maintain the desired protein in a stable state, the whole disruption process is usually carried out in a container containing ice water to help dissipate heat.
The current common ultrasonic crushing ice bath method in the laboratory is that ice blocks are taken in a common beaker, and then a centrifugal tube is put into crushed ice blocks for low-temperature ultrasonic crushing. The ultrasonication time is usually longer, and the ice-cube can be heated and melted in this in-process, if the ice-cube is not added in time, the melting of ice-cube can make the ultrasonication process can not fully dispel the heat in this in-process to the ice-cube melting can make the position change of centrifuging tube lead to the breakage insufficient, thereby makes the purpose protein productivity who obtains low.
Therefore, how to provide an ice bath device for ultrasonic crushing becomes a problem which needs to be solved by the technical personnel in the field.
SUMMERY OF THE UTILITY MODEL
In view of this, the utility model provides an ultrasonic crushing's ice bath device, simple structure, convenient to use not only can keep low temperature for a long time, has improved cooling efficiency, is favorable to improving crushing effect moreover.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
the utility model provides a broken ice bath device of supersound, includes ice bath mechanism, ice bath mechanism includes ice bath container and ice storage inner bag, ice storage inner bag inlays and installs in the ice bath container, ice storage inner bag top is provided with the recess, be provided with the centrifuging tube in the recess.
The centrifugal tube comprises a centrifugal tube body, and is characterized by further comprising an ultrasonic generator, an energy converter and an amplitude transformer, wherein the ultrasonic generator is electrically connected with the energy converter, one end of the amplitude transformer is assembled at the bottom end of the energy converter, and the other end of the amplitude transformer extends into the centrifugal tube. The ultrasonic generator drives the transducer to work, and the transducer drives the amplitude transformer to work, so that the cells are crushed.
Further, still include sound-proof box, ice bath mechanism sets up sound-proof box is interior to greatly reduced the noise that the ultrasonication in-process produced.
Furthermore, a water filling port is arranged on the ice storage inner container, tap water can be filled into the ice storage inner container through the water filling port, and then the ice storage inner container is placed into the freezing chamber for standby.
Further, the ice storage inner bag adopts cylindrical structure, the recess is located the edge of ice storage inner bag to the condition such as the position of the liquid level height of convenient observation centrifuging tube and transducer.
Furthermore, the ice storage inner container adopts a high-molecular water-absorbent resin inner container, and can absorb a large amount of water when water is injected into the ice storage inner container.
Further, the ice bath container adopts transparent glass container, can conveniently observe the liquid level height position of centrifuging tube and the position of transducer to reach the broken effect of ideal supersound.
The beneficial effects of the utility model reside in that:
the utility model has simple structure and convenient use, adopts the ice storage inner container to cool, not only can keep low temperature for a long time and improve the cooling efficiency, but also does not need the step of frequently adding ice blocks in the ice bath ultrasonic working process as in the prior art, reduces the workload of scientific research workers and improves the working efficiency; the groove is formed in the top end of the ice storage inner container, so that support is provided for the centrifugal tube, and the situation that the crushing effect is not ideal due to the fact that the distance between the transducer and the liquid level in the centrifugal tube is changed due to the fact that the centrifugal tube is displaced because ice cubes are heated and melted in the traditional ice bath ultrasonic crushing process can be avoided, and therefore the cell crushing effect is greatly improved; the ice storage inner container is arranged in the ice bath container, so that the ice storage inner container can be stably arranged.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
Fig. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a schematic structural diagram of the ice bath mechanism of the present invention.
Fig. 3 is a top view of the ice bath mechanism of the present invention.
Fig. 4 is a schematic structural view of the ice storage inner container of the present invention.
Wherein, in the figure,
1-ice bath container; 2-ice storage inner container; 21-a groove; 22-a water injection port; 3-centrifuging the tube; 4-ultrasonic generator; 5-a transducer; 6-an amplitude transformer; 7-a sound insulation box; 8-Ice bath mechanism.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to the attached drawings 1-4, the embodiment of the utility model discloses ultrasonic crushing's ice bath device, including ice bath mechanism 8, ice bath mechanism 8 includes ice bath container 1 and ice storage inner bag 2, and ice storage inner bag 2 inlays and installs in ice bath container 1, and 2 tops of ice storage inner bag are provided with recess 21, are provided with centrifuging tube 3 in the recess 21.
The utility model discloses still include supersonic generator 4, transducer 5 and become width of cloth pole 6, supersonic generator 4 and 5 electric connection of transducer, become the assembly of 6 one end of width of cloth pole in 5 bottoms of transducer, the other end stretches into in centrifuging tube 3. The ultrasonic generator 4 drives the transducer 5 to work, and the transducer 5 drives the amplitude transformer 6 to work, so that the cells are crushed.
The utility model discloses still include sound-proof box 7, ice bath mechanism 8 sets up in sound-proof box 7, and transducer 5 is installed on the inside top of sound-proof box 7 to greatly reduced the noise that produces among the ultrasonication process.
In another embodiment, the ice storage container 2 is provided with a water filling port 22, tap water can be filled into the ice storage container 2 through the water filling port 22, and then the ice storage container is put into a freezing chamber for standby, and is put into the freezing chamber at least 6 hours before each use. Tap water is injected from the main inlet 22 when the ice storage inner container 2 is used for the first time, the ice storage inner container is placed into a freezing chamber for freezing after being fully adsorbed, and then the ice storage inner container can be repeatedly used without injecting water.
In another embodiment, the ice storage container 2 is cylindrical, and the groove 21 is located at the edge of the ice storage container 2, so as to facilitate observation of the liquid level of the centrifugal tube 3 and the position of the transducer 5. In addition, the ice storage inner containers 2 with different shapes can be produced according to the size of the centrifugal tube 3, so that the groove 21 is matched with the centrifugal tube 3.
In another embodiment, the ice storage inner container 2 is made of a high molecular water absorbent resin inner container, and when water is injected into the ice storage inner container 2, a large amount of water can be absorbed, so that the ice storage inner container 2 can be repeatedly used.
In another embodiment, the ice bath container 1 is a transparent glass container, which can facilitate observing the liquid level of the centrifugal tube 3 and the position of the transducer 5, so as to achieve the desired ultrasonication effect. And the distance of the transducer 5 from the liquid level of the centrifuge tube 3 can be intuitively adjusted.
The utility model has simple structure and convenient use, adopts the ice storage inner container 2 for cooling, not only can keep low temperature for a long time and improve the cooling efficiency, but also does not need the step of frequently adding ice blocks in the ice bath ultrasonic working process as in the prior art, reduces the workload of scientific research workers and improves the working efficiency; the groove 21 is formed in the top end of the ice storage inner container 2, so that support is provided for the centrifugal tube 3, the situation that the crushing effect is not ideal due to the fact that the centrifugal tube 3 is shifted to change the distance between the transducer 5 and the liquid level in the centrifugal tube 3 in the traditional ice bath ultrasonic crushing process because ice cubes are heated and melted can be avoided, and the cell crushing effect is greatly improved; the ice storage inner container 2 is arranged in the ice bath container 1, so that the ice storage inner container 2 can be stably arranged.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (7)
1. The utility model provides a broken ice bath device of supersound, a serial communication port, includes ice bath mechanism, ice bath mechanism includes ice bath container and ice storage inner bag, the ice storage inner bag inlays the dress and is in the ice bath container, ice storage inner bag top is provided with the recess, be provided with the centrifuging tube in the recess.
2. An ultrasonic crushing ice bath apparatus according to claim 1, further comprising an ultrasonic generator, a transducer and a horn, wherein the ultrasonic generator is electrically connected to the transducer, and the horn is assembled at a bottom end of the transducer at one end and extends into the centrifuge tube at the other end.
3. The ultrasonically broken ice bath apparatus of claim 1 or 2, further comprising a sound isolation box, wherein the ice bath mechanism is disposed within the sound isolation box.
4. The ultrasonic crushing ice bath apparatus as claimed in claim 3, wherein the ice storage inner container is provided with a water filling port.
5. An ultrasonic crushing ice bath apparatus as claimed in claim 1 or 4, wherein the ice storage container is of cylindrical structure, and the groove is located at the edge of the ice storage container.
6. The ultrasonic crushing ice bath device as claimed in claim 5, wherein the ice storage inner container is made of a high molecular water absorbent resin inner container.
7. The ultrasonic crushing ice bath device according to claim 1, wherein the ice bath container is a transparent glass container.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921085731.5U CN210314290U (en) | 2019-07-11 | 2019-07-11 | Ultrasonic crushing's ice bath device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921085731.5U CN210314290U (en) | 2019-07-11 | 2019-07-11 | Ultrasonic crushing's ice bath device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210314290U true CN210314290U (en) | 2020-04-14 |
Family
ID=70150505
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921085731.5U Expired - Fee Related CN210314290U (en) | 2019-07-11 | 2019-07-11 | Ultrasonic crushing's ice bath device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210314290U (en) |
-
2019
- 2019-07-11 CN CN201921085731.5U patent/CN210314290U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200414 Termination date: 20210711 |
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| CF01 | Termination of patent right due to non-payment of annual fee |