CN210065777U

CN210065777U - Sequencer for chromosome detection

Info

Publication number: CN210065777U
Application number: CN201920656944.2U
Authority: CN
Inventors: 闭琼丽
Original assignee: Bi Qiongli
Current assignee: Bi Qiongli
Priority date: 2019-05-09
Filing date: 2019-05-09
Publication date: 2020-02-14
Anticipated expiration: 2029-05-09

Abstract

The utility model discloses a sequencer for chromosome detection, which comprises a frame, a sequencing platform and a machine body, wherein the machine body is provided with a beam, and the frame is also provided with a reagent liquid inlet device and a reagent supply device; the reagent feeding device comprises a first rotating shaft and a rotating disk, and the reagent supplying device comprises two mounting plates, a second rotating shaft and a reagent supporting plate; be equipped with a plurality of reagent installation section of thick bamboo between the top of mounting panel, leftmost reagent installation section of thick bamboo top is equipped with the slider, and the lower surface of slider is connected with the lift cylinder, is connected with the fixed plate on the piston rod of lift cylinder, and the fixed plate is connected with the vertical plate, and the vertical plate is connected with the diaphragm perpendicularly, is equipped with U type bayonet socket on the diaphragm. The utility model discloses can realize having improved appearance efficiency and chromosome detection efficiency greatly to test reagent's quick, accurate appearance of going up, make detection cycle shorten by a wide margin, realize the automation owing to advancing the appearance simultaneously, reduce the professional requirement to technical staff.

Description

Sequencer for chromosome detection

Technical Field

The utility model relates to a chromosome detects technical field, especially relates to a sequencer for chromosome detects.

Background

The main chemical components of chromosomes are deoxyribonucleic acid (DNA) and proteins. The DNA sequencing in the chromosome is a novel detection technology, can analyze and determine the complete sequence of genes, predict the possibility of suffering from various diseases, and reasonably predict individual behavior characteristics and behaviors, and can lock individual pathological change genes to prevent and treat in advance. In recent years, researches on DNA sequencing in chromosomes are mostly carried out around the development of gene chips, and remarkable achievements and developments are obtained, however, the conventional sequencers applied to clinical or research basically use large-scale factory-level or desktop-level sequencers, so that professional technicians are required to carry out manual sample loading operation, and the technical requirements are very high. The loading depends on the professional level of technicians, the loading amount cannot be accurately controlled in each loading process, the loading efficiency is extremely low, and the sequencing period is long. Due to the uncertainty of manual sample loading, the reagent amount loss is high, and the cost is high.

Disclosure of Invention

In order to solve the prior technical problem, the utility model provides a sequencer for chromosome detection.

In order to achieve the above object, the utility model provides a following technical scheme: a sequencer for chromosome detection comprises a base, a sequencing platform and a machine body, wherein the sequencing platform and the machine body are arranged on the base, a gene sequencing chip is arranged on the sequencing platform, a cross beam is arranged on the machine body, and a reagent inlet device and a reagent supply device are also arranged on the base;

the reagent liquid inlet device comprises a first rotating shaft and a rotating disc, the first rotating shaft is vertically and rotatably connected to the lower surface of a cross beam, the upper end of the first rotating shaft extends out of the cross beam and is connected with a first motor, the first motor is fixed on the cross beam, the rotating disc is fixed at the lower end of the first rotating shaft, a plurality of fixing through holes are uniformly distributed on the rotating disc at intervals along the axial direction, one fixing through hole faces a gene sequencing chip on a sequencing platform, the fixing through hole is a liquid inlet station, a liquid inlet cylinder is arranged above the liquid inlet station, the liquid inlet cylinder is fixed on the cross beam, and one fixing through hole adjacent to the liquid inlet station is a reagent supply station;

the reagent supply device is arranged on the right side of the reagent liquid inlet device and comprises two opposite mounting plates, a second rotating shaft and a reagent supporting plate; the mounting plates are fixed on the machine base through supports arranged at two ends of the mounting plates, the supports of the two mounting plates are arranged oppositely, a plurality of reagent mounting cylinders are uniformly arranged above the mounting plates at intervals, the number of the reagent mounting cylinders is consistent with that of the fixing through holes, a first snap ring and a second snap ring are sequentially arranged on the outer side surfaces of the reagent mounting cylinders from bottom to top, the diameter of the first snap ring is larger than the distance between the two mounting plates, the reagent mounting cylinders are clamped between the two mounting plates through the first snap ring, the reagent mounting cylinders are internally provided with the reagent cylinders, two second rotating shafts are respectively and rotatably arranged on the supports at two sides of the mounting plates, the second rotating shafts are connected with second motors, the second motors are fixed on the supports, connecting plates are respectively fixed on the second rotating shafts, the other ends of the connecting plates are connected with connecting rods, the reagent supporting plates are horizontally arranged, two ends of the, the reagent supporting plate is divided into reagent supporting grooves corresponding to the reagent mounting cylinders one by the baffles;

the slider is arranged above the leftmost reagent installation barrel, the slider is arranged on the cross beam in a sliding mode, the sliding direction of the slider is from the corresponding reagent installation barrel to the reagent supply station, the lower surface of the slider is connected with the lifting cylinder, the piston rod of the lifting cylinder is connected with the fixed plate, the fixed plate is connected with the longitudinal plate through the electric telescopic rod, the longitudinal plate is vertically connected with the transverse plate, the rear side of the longitudinal plate is vertically connected with the left side of the fixed plate, the bottom side of the longitudinal plate is vertically connected with the left side of the transverse plate, the transverse plate is provided with a U-shaped bayonet, and the U-shaped opening is matched.

Further, the reagent barrel comprises a first barrel body used for storing the reagent, a first push rod arranged at the upper end of the first barrel body and capable of sliding and stretching relative to the first barrel body, and a needle head arranged at the lower end of the first barrel body, wherein a first sealing piston is arranged at the bottom of the first push rod and arranged inside the first barrel body, a liquid outlet channel penetrating through the needle head along the axial direction of the needle head is arranged on the needle head, and the liquid outlet channel is communicated with the inside of the first barrel body.

Furthermore, be equipped with the recess that the notch is decurrent on the crossbeam, the recess internal rotation is equipped with the threaded rod, is equipped with the external screw thread on the threaded rod and its one end stretches out the crossbeam and is connected with the third motor, and the third motor is fixed on the crossbeam, be equipped with on the slider with external screw thread assorted screw hole, the slider passes through the screw hole and connects on the threaded rod.

The sequencing platform is characterized by further comprising a washing device, wherein a reagent inlet joint is arranged at the upper end of the sequencing platform, and the washing device comprises a washing fixed block, a washing reagent cylinder and a washing cylinder; the washing fixing block is fixed on one side of the sequencing platform, and is provided with an installation through hole for a washing reagent barrel to pass through; the flushing reagent barrel comprises a second barrel body penetrating through the installation through hole, a second push rod arranged at one end of the second barrel body and stretching and retracting relative to the second barrel body in a sliding mode, a second sealing piston is arranged at one end of the second push rod and arranged inside the second barrel body, the second barrel body is communicated with the reagent inlet connector through a flushing pipe, and a flushing cylinder is arranged on the outer side of the second push rod.

The utility model discloses during the use, control lift cylinder and electric telescopic handle earlier, make the leftmost reagent installation section of thick bamboo joint of mounting panel on the diaphragm, rotate the third motor afterwards, make the slider slide on the threaded rod, move to reagent supply station until reagent installation section of thick bamboo. The reagent installation cylinder is placed in the fixed through hole in the reagent supply station by controlling the lifting cylinder and the electric telescopic rod. Then a first motor drives the rotating disc to rotate by an angle of a fixed through hole station, so that the reagent installation cylinder is located at the liquid inlet station, the piston rod of the liquid inlet cylinder extends out, the first push rod of the reagent cylinder located at the liquid inlet station is pushed and pressed, and the test reagent in the reagent cylinder is quantitatively dropped on the gene test chip. The liquid inlet quantity of the test reagent at each time can be accurately controlled by controlling the stroke of the liquid inlet cylinder. Meanwhile, the reagent mounting barrel of the reagent supply device moves forwards by one reagent mounting barrel station under the driving of the second motor, and the reagent mounting barrel on the right side is supplemented into the leftmost position of the mounting plate and is conveyed to the reagent supply station through the sliding block. And after the reagent detection of one reagent cylinder is finished, the gene chip is washed by the washing cylinder.

The utility model discloses can realize the quick, accurate appearance of going up to test reagent, improve appearance efficiency and chromosome detection efficiency greatly, make detection cycle shorten by a wide margin, simultaneously because advance the appearance and realized the automation, reduced the professional requirement to technical staff to avoid the manual work to go up the appearance and lead to inaccurate and uncertainty, guaranteed the accuracy of testing result, still saved the reagent volume, reduced the testing cost.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

Fig. 2 is an enlarged schematic view at a in fig. 1.

Fig. 3 is an enlarged schematic view at B in fig. 1.

Fig. 4 is a top view of the rotation disc of the present invention.

Fig. 5 is a plan view of the reagent supplying apparatus of the present invention.

Fig. 6 is an axial sectional view of the reagent cartridge of the present invention.

Fig. 7 is a top view of the connection of the middle mounting plate, the longitudinal plate and the transverse plate.

In the figure: the device comprises a machine base 1, a sequencing platform 2, a machine body 3, a cross beam 4, a first rotating shaft 5, a rotating disc 6, a first motor 7, a fixing through hole 8, a liquid inlet station 9, a liquid inlet cylinder 10, a reagent supply station 11, a mounting plate 12, a second rotating shaft 13, a reagent supporting plate 14, a support 15, a reagent mounting barrel 16, a first clamping ring 17, a second clamping ring 18, a flushing pipe 19, a second motor 20, a connecting plate 21, a connecting rod 22, a baffle plate 23, a reagent bracket 24, a sliding block 25, a lifting cylinder 26, a fixing plate 27, a longitudinal plate 28, a transverse plate 29, a bayonet 30U, an electric telescopic rod 31, a first barrel 32, a first push rod 33, a needle 34, a holding tank 35, a needle hole 36, a groove 37, a threaded rod 38, a third motor 39, a reagent inlet joint 40, a flushing fixing block 41, a second push rod 42, a flushing cylinder 43.

Detailed Description

The embodiments of the present invention will be further explained with reference to the drawings.

Referring to fig. 1-6, a sequencer for chromosome detection comprises a base 1, a sequencing platform 2 arranged on the base 1, and a body 3. The sequencing platform 2 is provided with a gene sequencing chip (not shown in the figure). The gene sequencing chip obtains the number and sequence information of sample molecules after contacting with a test sample reagent. Be equipped with crossbeam 4 on the fuselage 3, still be equipped with reagent inlet means, reagent feeding device on the frame 1.

Reagent inlet means includes first pivot 5, rotary disk 6, first pivot 5 rotates perpendicularly and connects at crossbeam 4 lower surface, and the upper end of first pivot 5 stretches out crossbeam 4 and is connected with first motor 7, first motor 7 is fixed on crossbeam 4, rotary disk 6 is fixed at first pivot 5's lower extreme, and it has five fixed through hole 8 to follow axial interval evenly distributed on the rotary disk 6, and one of them fixed through hole 8 is the feed liquor station 9, feed liquor station 9 is just to the gene sequencing chip on sequencing platform 2, and the top of feed liquor station 9 is equipped with feed liquor cylinder 10, feed liquor cylinder 10 is fixed on crossbeam 4, and one of them fixed through hole 8 adjacent with feed liquor station 9 is reagent supply station 11. Under the drive of a first motor 7, a first rotating shaft 5 drives a rotating disc 6 to rotate, and five fixing through holes are alternately positioned at a liquid inlet station 9 or a reagent supply station 11 above a gene sequencing chip.

The reagent supply device is arranged on the right side of the reagent inlet device and comprises two opposite mounting plates 12, a second rotating shaft 13 and a reagent supporting plate 14. The mounting plates 12 are fixed on the base 1 through brackets 15 arranged at two ends of the mounting plates, the brackets 15 of the two mounting plates 12 are arranged oppositely, and five reagent mounting cylinders 16 are uniformly arranged above the mounting plates 12 at intervals. The lateral surface of a reagent installation section of thick bamboo 16 is equipped with first snap ring 17 and second snap ring 18 from supreme down in proper order, and the diameter of first snap ring 17 is greater than the distance between two mounting panels 12, and a reagent installation section of thick bamboo 16 is equipped with a reagent section of thick bamboo through first snap ring 17 card between two mounting panels 12 in the reagent installation section of thick bamboo 16. The reagent supporting plate is characterized in that the number of the second rotating shafts 13 is two, the second rotating shafts 13 are respectively rotatably arranged on the supports 15 on two sides of the mounting plate 12, the second rotating shafts 13 are connected with second motors 20, the second motors 20 are fixed on the supports 15, connecting plates 21 are respectively fixed on the second rotating shafts 13, the other ends of the connecting plates 21 are connected with connecting rods 22, the reagent supporting plate 14 is horizontally arranged, two ends of the reagent supporting plate are respectively rotatably connected with the two connecting rods 22, six baffle plates 23 are arranged above the reagent supporting plate 14, and the reagent supporting plate 14 is divided into reagent supporting grooves 24 which are in one-to-one correspondence with the. The second motor 20 drives the second rotating shaft 13 to rotate, and the connecting plate 21 rotates along with the second rotating shaft 13, so as to drive the reagent supporting plate 14 to reciprocate with a parallelogram track. When the connecting plate 21 rotates to the right side of the second rotating shaft 13, the reagent supporting plate 14 moves upwards until the reagent mounting cylinders on the mounting plates 12 are respectively positioned in the corresponding reagent supporting grooves 21, then the reagent mounting cylinder 16 moves leftwards along with the reagent supporting plate 14, when the connecting plate 21 rotates to the left side of the second rotating shaft 13, the reagent supporting plate 14 starts to move downwards, the reagent mounting cylinder 16 is clamped between the two mounting plates 12 through the first clamping ring 17, and at the moment, the reagent mounting cylinders 16 all move forwards by one reagent mounting cylinder station.

The slider 25 is arranged above the leftmost reagent mounting barrel 16, the slider 25 is arranged on the cross beam 4 in a sliding mode, the sliding direction of the slider 25 is from the corresponding reagent mounting barrel 16 to the reagent supply station 11, the lower surface of the slider 25 is connected with the lifting cylinder 26, the piston rod of the lifting cylinder 26 is connected with the fixed plate 27, the fixed plate 27 is connected with the longitudinal plate 28 through the electric telescopic rod 31, the longitudinal plate 28 is vertically connected with the transverse plate 29, the rear side of the longitudinal plate 28 is vertically connected with the left side of the fixed plate 27, the bottom side of the longitudinal plate 28 is vertically connected with the left side of the transverse plate 29, the transverse plate 29 is provided with the U-shaped bayonet 30, and the U-shaped opening 30 is matched with. The U-shaped bayonet 30 is provided on the front side of the fixing plate 27, thereby providing a space for accommodating the reagent cartridge 16. During operation, electric telescopic handle 31 stretches out to make the piston rod of lift cylinder 26 stretch out, when diaphragm 29 is located second snap ring 18 below, electric telescopic handle 31 contracts, makes reagent installation section of thick bamboo 16 place in the U type bayonet socket 30 of diaphragm 29. Then, the piston rod of the elevating cylinder 26 is contracted, and the reagent mounting cylinder 16 is raised. The reagent installation barrel 16 is clamped on the U-shaped bayonet 30 of the transverse plate 29 through the second clamping ring 18, so that the reagent installation barrel is connected with the sliding block 25 and slides along with the sliding block 25. The reagent mounting cartridge 16 on the mounting plate 12 is fed into the reagent supply station 11 of the rotary disk 6 by the sliding movement of the slider 25.

Based on the above technical solution, the reagent cartridge includes a first barrel 32 for storing reagent, a first push rod 33 disposed at an upper end of the first barrel 32 and sliding and extending relative to the first barrel 32, and a needle 34 installed at a lower end of the first barrel 32. The bottom of the first push rod 33 is provided with a first sealing piston (not shown in the figure), the first sealing piston is arranged inside the first cylinder 32, the needle 34 is provided with a liquid outlet channel which penetrates through the needle along the axial direction of the needle, and the liquid outlet channel is communicated with the inside of the first cylinder 32. An upward opening containing groove 35 for storing the reagent barrel is arranged in the reagent mounting barrel 16, and a needle hole 36 for storing a needle and dripping the reagent is arranged at the bottom of the containing groove 35. The beam 4 is provided with a liquid inlet cylinder 10 which is used for pushing a first push rod 33 of a reagent cylinder positioned at the liquid inlet station 9 to realize liquid inlet to the test platform 2.

The beam 4 is provided with a groove 37 with a downward notch, a threaded rod 38 is rotationally arranged in the groove 37, an external thread is arranged on the threaded rod 38, one end of the threaded rod extends out of the beam 4 and is connected with a third motor 39, the third motor 39 is fixed on the beam 4, a threaded hole matched with the external thread is arranged on the sliding block 25, and the sliding block 25 is connected to the threaded rod 38 through the threaded hole. In this embodiment, the length directions of the grooves 37, the threaded rods 38 and the mounting plate 12 are all the same as the radial direction of the rotary disk corresponding to the reagent supply station.

On the basis of the structure and the function, as an improvement, the sequencing platform connecting rod 2 further comprises a flushing device, a reagent inlet connector 40 is arranged at the upper end of the sequencing platform connecting rod 2, and the flushing device comprises a flushing fixing block 41, a flushing reagent barrel and a flushing air cylinder 43. Wash fixed block 41 and fix the one side at sequencing platform 2, wash and be equipped with on the fixed block 41 and be used for washing the installation through-hole that a reagent section of thick bamboo passed, wash a reagent section of thick bamboo including the second barrel 44 that passes the installation through-hole, locate second barrel 44 one end and for the second barrel 44 slip flexible second push rod 42, the one end of second push rod 42 is equipped with the sealed piston of second, the sealed piston of second is located inside second barrel 44, second barrel 44 passes through washing pipe 19 and reagent entry joint 41 intercommunication, wash the outside that second push rod 42 was located to cylinder 43. When washing, wash cylinder 43's piston rod and stretch out, promote second push rod 42, wash the interior washing reagent of reagent section of thick bamboo and extrude into sequencing platform 2 through wash pipe 19, wash gene chip, prevent to cause cross contamination between the reagent for the gene sequencing of difference, influence the precision of sequencing.

The utility model discloses during the use, control lift cylinder 26 and electric telescopic handle 31 earlier, make the 16 joints of the leftmost reagent installation section of thick bamboo of mounting panel 12 on diaphragm 29, rotate third motor 39 afterwards, make slider 25 slide on threaded rod 38, move to reagent supply station 11 until reagent installation section of thick bamboo 16. The reagent installation cylinder 16 is placed in the fixing through hole 8 at the reagent supply station 11 by controlling the lifting cylinder 26 and the electric telescopic rod 31. Then the first motor 7 drives the rotary disc 6 to rotate by an angle of a fixed through hole 8 station, so that the reagent installation cylinder 16 is positioned at the liquid inlet station 9, the piston rod of the liquid inlet cylinder 10 extends out, and the first push rod 33 of the reagent cylinder positioned at the liquid inlet station 9 is pushed and pressed, so that the test reagent in the reagent cylinder quantitatively drops on the gene test chip. The liquid inlet amount of the test reagent at each time can be accurately controlled by controlling the stroke of the liquid inlet cylinder 10. At the same time, the reagent mounting cylinder 16 of the reagent supply device is moved forward by the second motor 20 by one reagent mounting cylinder 16 station, and the right reagent mounting cylinder 16 is fed into the leftmost position of the mounting plate 12 and conveyed to the reagent supply station 11 by the slide 25. After the reagent detection of one reagent cartridge is completed, the gene chip is washed by the washing cylinder 43.

Claims

1. The utility model provides a sequencer for chromosome detects, includes the frame, locates sequencing platform and fuselage on the frame, be equipped with gene sequencing chip on the sequencing platform, be equipped with the crossbeam on the fuselage, its characterized in that: the base is also provided with a reagent inlet device and a reagent supply device;

2. A sequencer for chromosome detection according to claim 1, wherein: the reagent barrel comprises a first barrel body used for storing reagents, a first push rod arranged at the upper end of the first barrel body and capable of sliding and stretching relative to the first barrel body, and a needle head arranged at the lower end of the first barrel body, wherein a first sealing piston is arranged at the bottom of the first push rod and is arranged inside the first barrel body, a liquid outlet channel penetrating through the needle head along the axial direction of the needle head is arranged on the needle head, and the liquid outlet channel is communicated with the inside of the first barrel body.

3. A sequencer for chromosome detection according to claim 1, wherein: the novel motor is characterized in that a groove with a downward notch is formed in the cross beam, a threaded rod is arranged in the groove in a rotating mode, external threads are arranged on the threaded rod, one end of the threaded rod extends out of the cross beam and is connected with a third motor, the third motor is fixed on the cross beam, a threaded hole matched with the external threads is formed in the sliding block, and the sliding block is connected to the threaded rod through the threaded.

4. A sequencer for chromosome detection according to claim 1, wherein: the sequencing platform is characterized by further comprising a washing device, a reagent inlet joint is arranged at the upper end of the sequencing platform, and the washing device comprises a washing fixing block, a washing reagent barrel and a washing cylinder; the washing fixing block is fixed on one side of the sequencing platform, and is provided with an installation through hole for a washing reagent barrel to pass through; the flushing reagent barrel comprises a second barrel body penetrating through the installation through hole, a second push rod arranged at one end of the second barrel body and stretching and retracting relative to the second barrel body in a sliding mode, a second sealing piston is arranged at one end of the second push rod and arranged inside the second barrel body, the second barrel body is communicated with the reagent inlet connector through a flushing pipe, and a flushing cylinder is arranged on the outer side of the second push rod.