CN210012860U - Constant-temperature cell rotary incubator - Google Patents

Abstract

The utility model provides a rotatory incubator of constant temperature cell, include: the utility model discloses a box, the front side of box is provided with the chamber door, the fixed axial fan that is provided with on the outer wall of one side of box, the top of box is equipped with the gas vent, the below of box is equipped with the air inlet, still be provided with temperature control box on the box, be provided with main pivot on the box, the cover is equipped with solid fixed ring in the main pivot, gu fixed ring goes up the fixed bracing piece that is provided with the downward sloping, the bracing piece bottom is provided with blake bottle rotating electrical machines, be equipped with the rotation axis on the blake bottle rotating electrical machines, the rotation axis passes through cutting ferrule and ball-type blake bottle fixed connection, the side of box is provided with the bottle passageway of getting temporarily with the equal high department of ball-type blake bottle, the utility model discloses simple structure, but many batch cultured cells, the sterile environment.

Description

Constant-temperature cell rotary incubator

Technical Field

The utility model relates to a constant temperature cell culture case, in particular to constant temperature cell rotary incubator.

Background

In the cell suspension culture work, in order to ensure a good cell culture effect, it is necessary to ensure that: each cell has sufficient growth space, so that cell contact inhibition caused by mutual contact between cells is prevented, and cell growth is prevented; cells are uniformly dispersed in the culture solution, so that the subculture is convenient for uniform sampling; the cells and the culture solution are in full contact, so that the supply of nutrients required by the growth of the cells is ensured; the cells and the gas are in full contact, so that the gas metabolism requirement of the cells is ensured; the metabolite of cell in time will diffuse and get into the culture solution and be diluted by the culture solution, prevent that metabolite from gathering around the cell and inhibiting cell growth and hyperplasia, will guarantee the disturbance of culture solution through the external force in the cell culture process, promote the mass transfer in the cell culture system, so need use the rotatory incubator of constant temperature cell, should keep constant temperature, aseptic environment in the rotatory incubator of constant temperature cell, when taking out different culture bottles respectively inevitable can disturb the temperature in the incubator and get into bacterium, virus etc. and cause the influence to remaining culture bottles, thereby influence the culture effect.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the temperature that current constant temperature cell rotary incubator can disturb in the incubator inevitably and get into bacterium, virus etc. when taking out different blake bottles, cause the influence to all the other remaining blake bottles to influence cultivation effect scheduling problem provides a constant temperature cell rotary incubator, can be at the temperature stability that takes out the in-process of blake bottle in turn and keep the box, and maintain the sterile environment in the box.

The utility model provides a technical scheme does:

a thermostatted cell spin incubator comprising: the box body is provided with a box door at the front side, an axial flow fan is fixedly arranged on the outer wall of one side of the box body, an exhaust port is arranged above the box body, an air inlet is arranged below the box body, the box body is also provided with a temperature control box, the temperature control box is electrically connected with a first electric heating pipe, the first electric heating pipe is distributed on the inner wall of the box body, a heat preservation layer is arranged between the outer wall and the inner wall of the box body, supporting legs are fixedly arranged on the lower surface of the bottom of the box body, a main rotating shaft driving motor is fixedly arranged on the upper surface of the top of the box body, the main rotating shaft driving motor is connected with a driving gear through a driving rotating shaft, the driving gear drives a driven gear through a belt, the lower part of the driven gear is fixedly connected with the main rotating shaft, the, the cover is equipped with solid fixed ring in the main shaft, gu fixed ring is provided with a plurality ofly, from the top down arranges in proper order, gu fixed ring goes up the fixed bracing piece that is provided with the downward sloping, the bracing piece bottom is provided with blake bottle rotating electrical machines, be equipped with the rotation axis on the blake bottle rotating electrical machines, the rotation axis passes through cutting ferrule and ball-type blake bottle fixed connection, the side of box is provided with the bottle passageway of getting temporarily with the equal high department of ball-type blake bottle, the one end of getting the bottle passageway temporarily that is close to the box is provided with first shelves door, the one end of getting the bottle passageway temporarily that keeps away from the box is provided with second shelves door, it is provided.

Preferably, the spherical culture bottle is made of temperature-resistant glass materials.

Preferably, the bottom ends of the respective support bars are at the same horizontal plane.

Preferably, a second heating electric pipe is arranged between the inner wall and the outer wall of the temporary bottle taking channel.

Preferably, the edges of the box door, the first shutter and the second shutter are all provided with sealing strips.

Preferably, the door, the first stop door and the second stop door are all provided with insulating layers in a clamping manner.

The beneficial effects of the utility model are embodied in the following aspect:

1. simple structure and can culture cells in multiple batches.

2. And maintaining the sterile environment in the box body in the process of taking out the culture bottle.

3. The temperature in the box body is stable and has small fluctuation.

Drawings

FIG. 1 is a schematic view of the internal structure of the incubator.

FIG. 2 is a front view of the incubator.

1: a box body, 2: door, 3: axial flow fan, 4: exhaust port, 5: air inlet, 6: temperature control box, 7: first electric heating pipe, 8: insulating layer, 9: supporting legs, 10: main rotating shaft driving motor, 11: drive gear, 12: a belt, 13: driven gear, 14: main rotating shaft, 15: fixing ring, 16: support rod, 17: flask rotating motor, 18: rotation axis, 19: cutting ferrule, 20: ball-type culture flask, 21: temporary bottle access channel, 22: first shutter, 23: second door, 24 ultraviolet germicidal lamp, 25: a second heating electric tube.

Detailed Description

The present invention is further described in detail below with reference to the drawings so that those skilled in the art can implement the invention with reference to the description.

As shown in fig. 1-2, a constant temperature cell spin incubator comprises: the incubator comprises a box body 1, wherein a box door 2 is arranged on the front side of the box body 1, the box door 2 is hinged with the box body 1, when a large amount of cultures are placed in or taken out at one time, the box door 2 can pass through the box door, an axial flow fan 3 is fixedly arranged on the outer wall of one side of the box body 1, an exhaust port 4 is arranged above the box body 1, an air inlet 5 is arranged below the box body 1, hot air in the box body 1 is driven to flow through the exhaust port 4 and the air inlet 5, so that the uniform temperature field environment under the box is ensured, a temperature control box 6 is further arranged on the box body 1, the temperature control box 6 is electrically connected with a first electric heating pipe 7, the first electric heating pipe 7 is distributed on the inner wall of the box body 1, a heat preservation layer 8 is arranged between the outer wall and the inner wall of the box body 1, supporting legs 9, the main rotating shaft driving motor 10 is connected with the driving gear 11 through a driving rotating shaft, the driving gear 11 drives the driven gear 13 through the belt 12, the lower part of the driven gear 13 is fixedly connected with the main rotating shaft 14, the main rotating shaft 14 is rotatably connected with the top wall of the box body 1 through a bearing and penetrates through the box body 1 to enter the box body 1, the main rotating shaft 14 rotates under the interlocking action of the main rotating shaft driving motor 10 through the driving gear 11, the belt 12 and the driven gear 13, the belt 12 drives the main rotating shaft 14 to reduce the influence of the vibration of the main rotating shaft driving motor 10 on the main rotating shaft 14, the rotating speed of the main rotating shaft 14 can be adjusted by adjusting the diameter of the driving gear 11 and the driven gear 13, the main rotating shaft 14 is enabled to rotate more stably, the main rotating shaft 14 is sleeved with the fixing ring 15, the fixing ring 15 is provided with a plurality of rings, and, the fixing ring 15 is fixedly provided with a downwardly inclined supporting rod 16, the bottom end of the supporting rod 16 is provided with a culture bottle rotating motor 17, the culture bottle rotating motor 17 is provided with a rotating shaft 18, the rotating shaft 18 is fixedly connected with a spherical culture bottle 20 through a clamping sleeve 19, the spherical culture bottle 20 is made of temperature-resistant glass materials, the bottom end of each supporting rod 16 is positioned at the same horizontal plane, the side surface of the box body 1 is provided with a temporary bottle taking channel 21 at the same height as the spherical culture bottle 20, one end of the temporary bottle taking channel 21 close to the box body 1 is provided with a first gear door 22, one end of the temporary bottle taking channel 21 far away from the box body 1 is provided with a second gear door 23, an ultraviolet sterilizing lamp 24 is arranged above the inner wall of the temporary bottle taking channel 21, and a second heating electric tube 25 is arranged between the inner wall and the, the edge of chamber door 2, first shelves door 22 and second shelves door 23 all is provided with the sealing strip, all press from both sides in chamber door 2, first shelves door 22 and the second shelves door 23 and be equipped with heat preservation 8.

During the cultivation, the spherical culture bottle 20 rotates under the action of the culture bottle rotating motor 17 to ensure the disturbance of the culture solution and promote the mass transfer in a cell culture system, during the sampling, the main rotating shaft 14 is rotated, the spherical culture bottle 20 to be taken out is rotated to the position opposite to the temporary bottle taking channel 21, the first gear door 22 and the second gear door 23 are opened, the required spherical culture bottle 20 is taken out, the first gear door 22 and the second gear door 23 are closed in time, the air in the temporary bottle taking channel 21 is sterilized and disinfected by the ultraviolet sterilizing lamp 24, bacteria and viruses in the outside air are effectively blocked, the air entering the temporary bottle taking channel 21 is heated by the second heating electric tube 25, and the temperature of the air entering the box body 1 can be ensured to be consistent with the original temperature in the box body 1.

While embodiments of the invention have been disclosed above, it is not intended to be limited to the applications listed in the specification and the examples. It can be applicable to various and be fit for the utility model discloses a field completely. Additional modifications will readily occur to those skilled in the art. The invention is therefore not to be limited to the specific details and illustrations shown and described herein, without departing from the general concept defined by the claims and their equivalents.

Claims (6)

1. The utility model provides a constant temperature cell rotary incubator which characterized in that includes: the box body is provided with a box door at the front side, an axial flow fan is fixedly arranged on the outer wall of one side of the box body, an exhaust port is arranged above the box body, an air inlet is arranged below the box body, the box body is also provided with a temperature control box, the temperature control box is electrically connected with a first electric heating pipe, the first electric heating pipe is distributed on the inner wall of the box body, a heat preservation layer is arranged between the outer wall and the inner wall of the box body, supporting legs are fixedly arranged on the lower surface of the bottom of the box body, a main rotating shaft driving motor is fixedly arranged on the upper surface of the top of the box body, the main rotating shaft driving motor is connected with a driving gear through a driving rotating shaft, the driving gear drives a driven gear through a belt, the lower part of the driven gear is fixedly connected with the main rotating shaft, the, be equipped with solid fixed ring in the main pivot, gu fixed ring is provided with a plurality ofly, from the top down arranges in proper order, gu fixed ring goes up the fixed bracing piece that is provided with the downward sloping, the bracing piece bottom is provided with blake bottle rotating electrical machines, be equipped with the rotation axis on the blake bottle rotating electrical machines, the rotation axis passes through cutting ferrule and ball-type blake bottle fixed connection, the side of box is provided with the bottle passageway of getting temporarily with the equal high department of ball-type blake bottle, the one end of getting the bottle passageway temporarily that is close to the box is provided with first shelves door, the one end of getting the bottle passageway temporarily that keeps away from the box is provided with second shelves door, it is provided with.

2. The rotary incubator for the cells at constant temperature according to claim 1, wherein the ball-type culture flask is made of temperature-resistant glass material.

3. The incubator of claim 1, wherein the bottom ends of the support rods are located at the same horizontal plane.

4. The rotary incubator according to claim 1, wherein a second heating electric tube is disposed between the inner wall and the outer wall of the temporary bottle-taking channel.

5. The incubator of claim 1, wherein the door, the first shutter and the second shutter are provided with sealing strips at their edges.

6. The rotary incubator according to claim 5, wherein the door, the first door and the second door are all provided with insulating layers.

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