CN210001848U

CN210001848U - microbial cultivation jar

Info

Publication number: CN210001848U
Application number: CN201821634736.4U
Authority: CN
Inventors: 肖海清; 肖丹
Original assignee: Shenzhen Wohua Biotechnology Co Ltd
Current assignee: Shenzhen Wohua Biotechnology Co Ltd
Priority date: 2018-10-09
Filing date: 2018-10-09
Publication date: 2020-01-31
Anticipated expiration: 2028-10-09

Abstract

The utility model discloses a microorganism culture tank of kind includes a jar body, the top of this jar body has the sealed lid that can open and shut, sealed covering is seted up the inflation interface of switchable and the gassing interface of switchable at least, the microorganism culture tank of this application, cultivate the environmental gas with the microorganism and fill the jar internal of microorganism culture tank through the mode of aerifing, need not to adopt supporting evacuation equipment, also need not to use the gassing package, and is simple and convenient to use, and moreover, fill gas through the mode of aerifing, can also realize the mixture of multiple gas, can satisfy the microorganism culture demand that needs multiple mixed gas culture environment.

Description

microbial cultivation jar

Technical Field

The application relates to the field of microorganism anaerobic culture equipment, in particular to an microorganism culture tank.

Background

The microorganism culture tank is kinds of equipment for anaerobic culture of microorganisms, the conventional microorganism culture tank is generally sealed cylindrical tank bodies, air suction holes are formed in the tank bodies, when the microorganism culture tank is used, a culture medium inoculated with microorganisms is placed into the cylindrical tank bodies, meanwhile, a gas generation bag is placed into the cylindrical tank bodies, and then the cylindrical tank bodies are sealed and vacuumized to form an oxygen-free environment, wherein the gas generation bag is used for generating single nitrogen or carbon dioxide gas.

In the conventional microorganism culture tank, , matched vacuumizing equipment is required to be adopted for carrying out air extraction treatment, oxygen is required to be completely extracted from the microorganism culture tank to ensure an absolute anaerobic state, the requirements on the microorganism culture tank body and the vacuumizing equipment are high, in addition, the negative influence on a culture medium and microorganisms in the microorganism culture tank cannot be caused in the vacuumizing process, the high requirement on a gas production bag is also high, in the aspect of , the gas production bag in the conventional microorganism culture tank only can produce single gas, and for microorganisms with requirements of special culture conditions, the gas production bag for providing nitrogen or carbon dioxide in the single is difficult to meet the use requirements.

Disclosure of Invention

The purpose of the present application is to provide new microorganism culture tanks with improved structure.

The following technical scheme is adopted in the application:

the application discloses kind microorganism culture tank, it includes a jar body, and the top of jar body has the sealed lid that can open and shut, and sealed covering is seted up the interface of aerifing of at least switchable and the interface of bleeding of at least switchable.

The microorganism culture tank is different from the conventional microorganism culture tank in that at least two interfaces, inflation interfaces and deflation interfaces are arranged, when the conventional microorganism culture tank is used, the vacuum pumping equipment is used for pumping air to realize an anaerobic environment, when the microorganism culture tank is used, the anaerobic gas is directly filled in the inflation interfaces, the oxygen is discharged from the deflation interfaces, the whole exhaust process is realized, the microorganism culture tank is carried out under normal pressure, the use is simple and convenient, after the inflation and the exhaust for a plurality of seconds, the deflation interfaces are closed, so that the microorganism culture tank is filled with the anaerobic gas, and the microorganism culture tank fills the anaerobic gas in the microorganism culture tank instead of the gas generation bag, so that the microorganism culture tank can be prepared with mixed gas in different proportions in advance outside the microorganism culture tank according to the use requirement, and then fills the mixed gas in the microorganism culture tank, or directly replaces the mixed gas to form the mixed gas, and the mixed gas can be filled in the mixed gas to meet the use requirement of the microorganism culture tank.

Preferably, the microorganism culture tank of the application is further provided with a pressure gauge for displaying the air pressure in the tank body.

In implementations of the present application, a pressure gauge is disposed on a surface of the sealing cap to facilitate observation of the pressure inside the microorganism culture tank, and in aspects, after the oxygen is discharged and the air release port is closed, the amount of air inflation can be controlled according to the pressure displayed by the pressure gauge, for example, the air inflation is stopped after the air inflation reaches a set pressure, and the air inflation port is closed, and in aspects, the pressure gauge can observe the change of the pressure inside the tank during the microorganism culture.

Preferably, the sealing cover and the tank body are sealed by a sealing ring.

Preferably, the sealing cover is fastened at the top of the tank body by adopting a cross-shaped support or an -shaped support, specifically, the cross-shaped support or the -shaped support is fixed with the tank body in a clamping manner, a screwing screw is arranged on the cross-shaped support or the -shaped support, and after the cross-shaped support or the -shaped support is clamped on the tank body, the sealing cover is pressed and fastened at the top of the tank body by rotating the screwing screw.

In implementations of the present application, a total of four tightening screws are provided on the cross-shaped bracket, the four tightening screws are respectively provided on four supporting arms of the cross-shaped bracket, and the sealing cover is tightly pressed by the four tightening screws to be fastened on the top of the tank body.

The beneficial effect of this application lies in:

according to the microbial culture tank, the microbial culture environment gas is filled into the tank body of the microbial culture tank in an inflation mode, matched vacuum pumping equipment is not needed, a gas generation bag is not needed, and the microbial culture tank is simple and convenient to use; moreover, the gas is filled in the culture medium in an inflation mode, so that the mixing of multiple gases can be realized, and the requirement of microbial culture in multiple mixed gas culture environments can be met.

Drawings

FIG. 1 is a schematic side view of a microorganism culture tank according to an embodiment of the present application;

FIG. 2 is a schematic side sectional view of a microorganism culture tank in the embodiment of the present application;

FIG. 3 is a schematic view showing the structure of a top sealing lid of a microorganism culture tank in the embodiment of the present application;

FIG. 4 is a schematic perspective view of another kinds of microorganism culture tanks according to the embodiment of the present application;

FIG. 5 is a schematic view showing an exploded structure of another kinds of microorganism culture tanks in the example of the present application.

Detailed Description

The present application is further described in detail below in by way of specific examples the following examples are illustrative of the present application only in and should not be construed as limiting the present application.

Examples

The microorganism culture tank comprises a tank body 1, a sealing cover 2 which can be opened and closed is arranged at the top of the tank body 1, the sealing cover 2 and the tank body 1 are sealed by sealing rings, switchable inflation connectors 21 and switchable deflation connectors 22 are arranged on the sealing cover 2, pressure gauges 3 are further arranged on the surface of the sealing cover 2 and used for displaying air pressure in the tank body 1, the sealing cover 2 of the embodiment is tightly buckled at the top of the tank body 1 by a cross-shaped support 4, concretely, the cross-shaped support 4 is fixed with the tank body 1 in a clamping mode, four screwing screws 41 are arranged on the cross-shaped support 4 and are respectively arranged on four supporting arms of the cross-shaped support, and after the cross-shaped support 4 is clamped on the tank body 1, the sealing cover 2 is pressed and buckled at the top of the tank body 1 by rotating the screwing screws 41.

When the microorganism culture tank is used, the screwing screw is firstly rotated to unscrew the screwing screw, the sealing cover is taken down, and then a culture is placed in the tank body; tightly covering the sealing cover, and opening the inflation interface and the deflation interface; the inflation interface is connected with an air source or a mixed air source to inflate the tank body, air and oxygen are discharged during inflation due to the fact that the deflation interface is opened, the process lasts for several seconds or tens of seconds approximately, the air can be inflated into the tank body, the oxygen is completely removed, and the specific time is determined according to the inflation speed and the size of the tank body; after the oxygen is discharged, the air discharging interface is closed, the air is continuously inflated until the air pressure in the tank body reaches a set air pressure value, and the air charging interface is closed. And finally, placing the microorganism culture tank in a constant-temperature incubator for microorganism culture.

In another implementation manners of this example, the -shaped bracket 5 is adopted to fasten the sealing cover on the top of the tank body, as shown in fig. 4 and 5, the -shaped bracket is designed with screws 51 at its symmetrical center, the fixing manner of the -shaped bracket 5 is similar to that of the cross-shaped bracket, and the rest of the structure is the same, which will not be described herein.

Although the present embodiment specifically provides two bracket types, i.e., a cross-shaped bracket and an -shaped bracket, it is understood that under the inventive concept of the present application, various types or structures of brackets can be designed to meet individual requirements of different products, and are not limited in detail herein.

It will be apparent to those skilled in the art from this disclosure that numerous modifications and variations can be made without departing from the spirit or scope of the disclosure as defined in claim .

Claims

The microbial cultivation tank comprises a tank body (1) and is characterized in that a sealing cover (2) capable of being opened and closed is arranged at the top of the tank body (1), at least switchable inflation connectors (21) and at least switchable deflation connectors (22) are arranged on the sealing cover (2), the sealing cover (2) is tightly buckled at the top of the tank body (1) through a cross-shaped support (4) or a -shaped support (5), specifically, the cross-shaped support (4) or the -shaped support (5) is fixed with the tank body (1) in a clamping mode, tightening screws (41 and 51) are arranged on the cross-shaped support (4) or the -shaped support (5), and after the cross-shaped support (4) or the -shaped support (5) is clamped on the tank body (1), the sealing cover (2) is pressed and buckled at the top of the tank body (1) through rotating the tightening screws (41 and 51).
2. The microbial cultivation tank of claim 1, wherein: and a pressure gauge (3) is also arranged and used for displaying the air pressure in the tank body (1).
3. A microbial cultivation tank as claimed in claim 1 or 2, wherein: and a sealing ring is adopted between the sealing cover (2) and the tank body (1) for sealing.