CN207391418U - A kind of disposable anaerobic bacteria Liquid Culture test tube - Google Patents
A kind of disposable anaerobic bacteria Liquid Culture test tube Download PDFInfo
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- CN207391418U CN207391418U CN201721346184.2U CN201721346184U CN207391418U CN 207391418 U CN207391418 U CN 207391418U CN 201721346184 U CN201721346184 U CN 201721346184U CN 207391418 U CN207391418 U CN 207391418U
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- 238000012360 testing method Methods 0.000 title claims abstract description 72
- 238000009630 liquid culture Methods 0.000 title claims abstract description 24
- 241001148471 unidentified anaerobic bacterium Species 0.000 title claims abstract description 23
- 238000007789 sealing Methods 0.000 claims abstract description 41
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 26
- 239000001301 oxygen Substances 0.000 claims abstract description 26
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 26
- 229940123973 Oxygen scavenger Drugs 0.000 claims abstract description 25
- 238000006392 deoxygenation reaction Methods 0.000 claims abstract description 21
- 239000012528 membrane Substances 0.000 claims description 6
- 239000011248 coating agent Substances 0.000 claims description 4
- 238000000576 coating method Methods 0.000 claims description 4
- 230000007547 defect Effects 0.000 abstract description 3
- 239000004033 plastic Substances 0.000 abstract description 3
- 238000004140 cleaning Methods 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 230000001954 sterilising effect Effects 0.000 abstract 1
- 238000004659 sterilization and disinfection Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 4
- 230000008020 evaporation Effects 0.000 description 3
- 238000001704 evaporation Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
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Abstract
本实用新型提供了一种一次性厌氧菌液体培养试管，包括试管和旋盖；所述旋盖内设置有除氧盒和密封层；所述除氧盒为中空的圆柱体或长方体结构，底面或侧面至少一面具有开口，所述除氧盒包括除氧剂和透气膜，所述除氧剂填充于除氧盒内，所述透气膜固定并覆盖于所述除氧盒的开口上；所述除氧盒固定于所述旋盖的顶面上；所述密封层在除氧盒外。本一次性厌氧菌液体培养试管，通过除氧盒的设置解决了试管内部厌氧环境提供不充分的缺陷；本实用新型采用塑料试管，一次性使用，结构简单，既适于产业化批量生产，又避免了清洗灭菌步骤，提高了实际检测时的工作效率。
The utility model provides a disposable anaerobic bacteria liquid culture test tube, which comprises a test tube and a screw cap; a deoxygenation box and a sealing layer are arranged in the screw cap; the deoxygenation box is a hollow cylinder or a cuboid structure, The bottom surface or at least one side has an opening, and the deoxygenation box includes an oxygen scavenger and a breathable film, the oxygen scavenger is filled in the oxygen scavenger box, and the breathable film is fixed and covered on the opening of the oxygen scavenger box; The oxygen removal box is fixed on the top surface of the screw cap; the sealing layer is outside the oxygen removal box. The disposable anaerobic bacteria liquid culture test tube solves the defect of insufficient anaerobic environment inside the test tube through the setting of the deoxygenation box; the utility model adopts a plastic test tube, which is disposable and simple in structure, and is suitable for industrialized mass production , avoiding the steps of cleaning and sterilization, and improving the work efficiency during actual detection.
Description
技术领域technical field
本实用新型涉及一种试验仪器，具体涉及一种一次性厌氧菌培养试管。The utility model relates to a test instrument, in particular to a disposable anaerobic bacteria culture test tube.
背景技术Background technique
厌氧微生物在自然界分布广泛，种类繁多，作用也日益引起重视。但是，实验室一般条件下培养，这些厌氧菌不能生长，对其研究需要产生无氧环境的特殊设备。对于厌氧菌的液体培养，目前主要有两种方式，一种是采用厌氧罐，将菌种接种到含液体培养基的试管内，连接厌氧设备，利用仪器除去厌氧罐内氧气；另一种是亨盖特厌氧玻璃试管，采用注射器抽真空方式提供厌氧环境。以上两种方法，在实际应用过程中都存在一定缺陷，厌氧罐方式提供厌氧环境，由于试管采用橡胶塞盖住，形成试管内一个相对独立的环境，不利于对试管内部形成良好的厌氧环境，且设备较为昂贵；第二种亨盖特试管，是一种玻璃试管，每次使用都需要清洗、灭菌，操作起来非常繁琐不适于大批量厌氧菌的培养。Anaerobic microorganisms are widely distributed in nature, and there are many kinds, and their role has attracted increasing attention. However, these anaerobic bacteria cannot grow under normal conditions in the laboratory, and their research requires special equipment that produces an anaerobic environment. For the liquid culture of anaerobic bacteria, there are currently two main methods. One is to use anaerobic tanks, inoculate the bacteria into test tubes containing liquid medium, connect anaerobic equipment, and use instruments to remove oxygen in the anaerobic tanks; The other is the Hungate anaerobic glass test tube, which uses syringe vacuum to provide an anaerobic environment. The above two methods have certain defects in the actual application process. The anaerobic tank method provides an anaerobic environment. Since the test tube is covered with a rubber stopper, a relatively independent environment in the test tube is formed, which is not conducive to forming a good anaerobic environment inside the test tube. Oxygen environment, and the equipment is relatively expensive; the second type of Hungate test tube is a glass test tube, which needs to be cleaned and sterilized every time it is used. The operation is very cumbersome and is not suitable for the cultivation of large quantities of anaerobic bacteria.
发明内容Contents of the invention
针对目前缺乏高效简便去除氧气的厌氧菌液体培养试管的问题，本实用新型提供一种一次性厌氧菌液体培养试管，携带和使用方便、成本低。Aiming at the current problem of lacking an anaerobic bacteria liquid culture test tube that is efficient and simple to remove oxygen, the utility model provides a disposable anaerobic bacteria liquid culture test tube, which is convenient to carry and use and low in cost.
为实现上述目的，本实用新型采用如下技术方案。In order to achieve the above object, the utility model adopts the following technical solutions.
一次性厌氧菌液体培养试管，包括试管和旋盖；所述试管为上端开口、底部密封的空腔圆柱结构，由管壁和管底组成；所述管壁上具有螺纹A，螺纹A位于试管开口处；所述旋盖为T形或倒U形结构，侧壁上具有与管壁的螺纹A相配合的螺纹B；所述旋盖内设置有除氧盒和密封层；Disposable liquid culture test tube for anaerobic bacteria, including a test tube and a screw cap; the test tube is a cavity cylindrical structure with an open upper end and a sealed bottom, consisting of a tube wall and a tube bottom; the tube wall has a thread A, and the thread A is located at The opening of the test tube; the screw cap is a T-shaped or inverted U-shaped structure, and the side wall has a thread B that matches the thread A of the tube wall; a deoxygenation box and a sealing layer are arranged inside the screw cap;
所述除氧盒为中空的圆柱体或长方体结构，底面或侧面至少一面具有开口，所述除氧盒包括除氧剂和透气膜，所述除氧剂填充于除氧盒内，所述透气膜固定并覆盖于所述除氧盒的开口上，除氧剂可通过透气膜与外界空气连通，所述除氧盒固定于所述旋盖的顶面上；所述密封层在除氧盒外，将除氧剂与外界空气分离阻隔。The deoxygenation box is a hollow cylinder or cuboid structure, with openings on at least one side of the bottom or side, the oxygen scavenger box includes an oxygen scavenger and a gas-permeable membrane, the oxygen scavenger is filled in the oxygen-scavenger box, and the gas-permeable The film is fixed and covered on the opening of the deoxygenation box, and the oxygen scavenger can communicate with the outside air through the gas-permeable film, and the deoxygenation box is fixed on the top surface of the screw cap; In addition, the oxygen scavenger is separated from the outside air.
所述密封层优选为密封套或密封膜；所述密封套罩于所述除氧盒外，上端开口封闭并固定于旋盖顶面；所述密封膜周边贴于除氧盒的开口周边，覆盖在透气膜外。The sealing layer is preferably a sealing sleeve or a sealing film; the sealing sleeve is covered outside the oxygen removal box, and the upper opening is closed and fixed on the top surface of the screw cap; the periphery of the sealing film is attached to the opening periphery of the oxygen removal box, covered by a breathable membrane.
作为优选，旋盖上还设有密封垫，所述密封垫的尺寸与所述管壁相互匹配；当管壁的螺纹A为内螺纹，旋盖侧壁的螺纹B为外螺纹时，所述旋盖的侧壁外套设有密封垫；当所述管壁螺纹A为外螺纹，旋盖的内壁的螺纹B为外螺纹时，所述旋盖的顶面内侧固定有环状的密封垫。旋盖旋紧后密封垫与试管开口完全密闭，可防止漏气和水分蒸发。Preferably, a sealing gasket is also provided on the screw cap, and the size of the sealing gasket matches the pipe wall; when the thread A of the pipe wall is an internal thread, and the thread B of the side wall of the screw cap is an external thread, the The side wall of the screw cap is provided with a sealing gasket; when the thread A of the pipe wall is an external thread and the thread B of the inner wall of the screw cap is an external thread, a ring-shaped sealing gasket is fixed inside the top surface of the screw cap. After the cap is screwed tightly, the sealing gasket and the test tube opening are completely sealed to prevent air leakage and water evaporation.
所述管底为平底、圆底或锥形底，作为优选，所述管底为圆底或锥形底时，一次性厌氧菌液体培养试管还包括试管座，所述试管座为所述管壁自管底处向下延伸形成的筒状结构。The bottom of the tube is a flat bottom, a round bottom or a conical bottom. As a preference, when the bottom of the tube is a round bottom or a conical bottom, the disposable anaerobic bacteria liquid culture test tube also includes a test tube holder, and the test tube holder is the A cylindrical structure formed by the pipe wall extending downward from the bottom of the pipe.
作为优选，管壁上还具有刻度或标记涂层的至少一种。Preferably, the tube wall also has at least one of a graduated or marking coating.
所述一次性厌氧菌液体培养试管，由管壁与旋盖由塑料制成，整体经环氧乙烷或γ射线等方式杀菌后，制成密封包装。使用时，撕开包装，打开试管，注入灭菌液体培养基，接入菌体，轻轻混匀后，移除除氧盒上的密封层，迅速旋紧旋盖，使除氧剂与试管内空气接触，除氧盒内的除氧剂可自行将试管内氧气反应消耗掉，从而提供试管内部处于无氧环境。The disposable anaerobic bacteria liquid culture test tube is made of plastic with a tube wall and a screw cap, and the whole is sterilized by means of ethylene oxide or gamma rays, and then made into a sealed package. When in use, tear open the package, open the test tube, inject the sterilized liquid medium, insert the bacteria, mix gently, remove the sealing layer on the oxygen removal box, and quickly tighten the screw cap to make the oxygen scavenger and the test tube In contact with the air, the oxygen scavenger in the oxygen removal box can consume the oxygen reaction in the test tube by itself, so as to provide an oxygen-free environment inside the test tube.
本实用新型具有以下优点：The utility model has the following advantages:
本实用新型提供的一次性厌氧菌液体培养试管，管盖的除氧盒可对试管内环境除氧，解决了厌氧罐试管内部厌氧环境提供不充分的缺陷；另外，本实用新型采用塑料试管，经一次性无菌处理后密封，可以直接使用，使用后无害化处理废弃即可，既适于产业化批量生产该试管，又免去了清洗灭菌的步骤，可大大提高实际检测时的工作效率。本一次性厌氧菌液体培养试管可广泛应用于大批量液体培养厌氧菌的工作中。In the disposable anaerobic liquid culture test tube provided by the utility model, the deoxygenation box of the tube cover can deoxygenate the environment in the test tube, which solves the defect that the anaerobic environment inside the anaerobic tank test tube is insufficiently provided; in addition, the utility model adopts The plastic test tube is sealed after one-time aseptic treatment, and can be used directly. Work efficiency during testing. The disposable anaerobic bacteria liquid culture test tube can be widely used in the work of mass liquid culture of anaerobic bacteria.
附图说明Description of drawings
图1为实施例1中一次性厌氧菌液体培养试管示意图与旋盖纵剖面示意图；1 is a schematic diagram of a disposable anaerobic liquid culture test tube and a schematic longitudinal section of a screw cap in Example 1;
图2为实施例中带有密封膜的旋盖仰视示意图；Fig. 2 is a schematic bottom view of a screw cap with a sealing film in an embodiment;
图3为实施例中除去密封膜的旋盖仰视示意图；Fig. 3 is a schematic bottom view of the screw cap that removes the sealing film in the embodiment;
图4为实施例2中一次性厌氧菌液体培养试管示意图与旋盖纵剖面示意图；4 is a schematic diagram of a disposable anaerobic liquid culture test tube and a schematic longitudinal section of a screw cap in Example 2;
其中，1-试管，2-旋盖，21-除氧盒，211-透气膜，212-除氧剂，22-密封膜，3-管壁，31-刻度，32-标识涂层，4-管底，5-螺纹A，6-螺纹B，7-密封垫，8-试管座。Among them, 1-test tube, 2-screw cap, 21-deoxygenation box, 211-breathable film, 212-deoxygenation agent, 22-sealing film, 3-tube wall, 31-scale, 32-logo coating, 4- Tube bottom, 5-thread A, 6-thread B, 7-sealing gasket, 8-test tube holder.
具体实施方式Detailed ways
下面结合实施例和附图对本实用新型做进一步说明，但本实用新型不受下述实施例的限制。Below in conjunction with embodiment and accompanying drawing, the utility model is further described, but the utility model is not limited by following embodiment.
实施例1Example 1
如图1-3所示的一次性厌氧菌液体培养试管，包括试管（1）和旋盖（2）；所述试管（1）为上端开口、底部密封的空腔圆柱结构，由管壁（3）和管底（4）组成；所述试管（1）开口处的管壁（3）外侧具有螺纹A（5）；所述管壁（3）上还具有标识容积的刻度（31）和便于标记的标记涂层（32）；所述管底（4）为圆锥形；所述管壁（3）自管底（4）向下延伸形成的筒状的试管座（8）；所述旋盖（2）为倒U形结构，侧壁上具有与管壁（3）螺纹A（5）相配合的螺纹B（6）；所述旋盖（2）内设置有除氧盒（21）、密封膜（22）和密封垫（7）；所述除氧盒（21）为中空的圆柱状结构，底面有圆形开口，所述除氧盒（21）包括除氧剂（211）和透气膜（212），所述除氧剂（211）填充于除氧盒内，所述透气膜（212）固定并覆盖于除氧盒（21）底面开口内侧，除氧剂（211）可通过透气膜（212）与外界空气连通，所述除氧盒（21）固定于所述旋盖（2）的顶面上；所述密封膜（22）周边贴于除氧盒（21）开口周边，覆盖在透气膜（212）外，将除氧剂（211）与外界空气分离阻隔；所述密封垫（7）为环状，固定于旋盖（2）的顶面内侧旋盖（2）侧壁与除氧盒（21）之间，旋盖（2）旋紧后密封垫（7）与试管（1）开口完全密闭，可防止漏气和水分蒸发。使用时，移除除氧盒上的密封膜，迅速旋紧旋盖，使除氧剂与试管内空气接触，将试管内氧气反应掉，从而提供试管内部处于无氧环境。The disposable anaerobic liquid culture test tube as shown in Figure 1-3 includes a test tube (1) and a screw cap (2); the test tube (1) is a hollow cylindrical structure with an open upper end and a sealed bottom. (3) and tube bottom (4); the tube wall (3) at the opening of the test tube (1) has thread A (5) on the outside; the tube wall (3) also has a scale (31) marking the volume and a marking coating (32) for easy marking; the bottom of the tube (4) is conical; the tube wall (3) is a cylindrical test tube seat (8) formed by extending downward from the bottom of the tube (4); The screw cap (2) is an inverted U-shaped structure, and the side wall has a thread B (6) matching with the thread A (5) of the pipe wall (3); the screw cap (2) is provided with a deoxygenation box ( 21), a sealing film (22) and a sealing gasket (7); the oxygen removal box (21) is a hollow cylindrical structure with a circular opening on the bottom, and the oxygen removal box (21) includes an oxygen scavenger (211 ) and gas-permeable film (212), the oxygen scavenger (211) is filled in the oxygen-scavenging box, the gas-permeable film (212) is fixed and covered on the inner side of the bottom opening of the oxygen-scavenger box (21), and the oxygen-scavenger (211) It can communicate with the outside air through a breathable membrane (212), the deoxygenation box (21) is fixed on the top surface of the screw cap (2); the periphery of the sealing film (22) is attached to the deoxygenation box (21) The periphery of the opening is covered by the gas-permeable membrane (212) to separate and block the oxygen scavenger (211) from the outside air; the sealing gasket (7) is ring-shaped and fixed on the top surface of the screw cap (2) inside the screw cap ( 2) Between the side wall and the oxygen removal box (21), after the screw cap (2) is tightened, the sealing gasket (7) and the opening of the test tube (1) are completely sealed to prevent air leakage and water evaporation. When in use, remove the sealing film on the oxygen removal box, quickly tighten the screw cap, make the oxygen removal agent contact with the air in the test tube, and react the oxygen in the test tube, so as to provide an oxygen-free environment inside the test tube.
实施例2Example 2
如图2-4所示的一次性厌氧菌液体培养试管，包括试管（1）和旋盖（2）；所述试管（1）为上端开口底部密封的空腔圆柱结构，由管壁（3）和管底（4）组成；所述试管（1）开口处的管壁（3）内侧具有螺纹A（5）；所述管壁（3）上还具有标识容积的刻度（31）；所述管底（4）为圆底；所述管壁（3）自管底（4）向下延伸形成的筒状的试管座（8）；所述旋盖（2）为T形结构，侧壁上具有与管壁（3）螺纹A（5）相配合的螺纹B（6）；所述旋盖（2）的侧壁外套设有密封垫（7），所述密封垫（7）的尺寸与所述试管（1）开口相互匹配；旋盖（2）旋紧后密封垫（7）与试管（1）开口完全密闭，可防止漏气和水分蒸发；所述旋盖（2）内设置有除氧盒（21）和密封膜（22）；所述除氧盒（21）为中空的圆柱状结构，底面有圆形开口；所述除氧盒（21）包括除氧剂（211）和透气膜（212），所述除氧剂（211）填充于所述除氧盒（21）内，所述透气膜（212）固定并覆盖于除氧盒（21）底面开口内侧，除氧剂（211）可通过透气膜（212）与外界空气连通，所述除氧盒（21）固定于所述旋盖（2）的顶面上；所述密封膜（22）周边贴于除氧盒（21）开口周边，覆盖在透气膜（212）外，将除氧剂（211）与外界空气分离阻隔。使用时，移除除氧盒上的密封膜，迅速旋紧旋盖，使除氧剂与试管内空气接触，将试管内氧气反应掉，从而提供试管内部处于无氧环境。The disposable anaerobic bacteria liquid culture test tube shown in Figure 2-4 includes a test tube (1) and a screw cap (2); the test tube (1) is a hollow cylindrical structure with an open upper end and a sealed bottom, and the tube wall ( 3) composed of a tube bottom (4); the inside of the tube wall (3) at the opening of the test tube (1) has a thread A (5); the tube wall (3) also has a scale (31) marking the volume; The tube bottom (4) is a round bottom; the tube wall (3) is a cylindrical test tube seat (8) formed by extending downward from the tube bottom (4); the screw cap (2) is a T-shaped structure, The side wall has a thread B (6) matching with the thread A (5) of the pipe wall (3); the side wall of the screw cap (2) is provided with a gasket (7), and the gasket (7) The size of the test tube (1) is matched with the opening of the test tube (1); after the screw cap (2) is tightened, the sealing gasket (7) is completely sealed with the test tube (1) opening, which can prevent air leakage and water evaporation; the screw cap (2) A deoxygenation box (21) and a sealing film (22) are arranged inside; the deoxygenation box (21) is a hollow cylindrical structure with a circular opening on the bottom; the deoxygenation box (21) includes an oxygen scavenger ( 211) and a breathable film (212), the oxygen scavenger (211) is filled in the oxygen removal box (21), and the breathable film (212) is fixed and covered on the inside of the bottom opening of the oxygen removal box (21), The oxygen scavenger (211) can communicate with the outside air through the gas-permeable film (212), and the oxygen scavenger box (21) is fixed on the top surface of the screw cap (2); the periphery of the sealing film (22) is pasted on The periphery of the opening of the deoxygenation box (21) is covered with a gas permeable membrane (212) to separate and block the deoxygenation agent (211) from the outside air. When in use, remove the sealing film on the oxygen removal box, quickly tighten the screw cap, make the oxygen removal agent contact with the air in the test tube, and react the oxygen in the test tube, so as to provide an oxygen-free environment inside the test tube.
Claims (8)
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721346184.2U CN207391418U (en) | 2017-10-19 | 2017-10-19 | A kind of disposable anaerobic bacteria Liquid Culture test tube |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201721346184.2U CN207391418U (en) | 2017-10-19 | 2017-10-19 | A kind of disposable anaerobic bacteria Liquid Culture test tube |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110683173A (en) * | 2019-11-01 | 2020-01-14 | 安徽创孚医疗科技有限公司 | Deoxidization cryopreserving device |
| CN112795465A (en) * | 2021-03-12 | 2021-05-14 | 北京大学深圳研究生院 | Novel anaerobe liquid medium deoxidization device |
-
2017
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110683173A (en) * | 2019-11-01 | 2020-01-14 | 安徽创孚医疗科技有限公司 | Deoxidization cryopreserving device |
| CN110683173B (en) * | 2019-11-01 | 2024-08-23 | 安徽创孚医疗科技有限公司 | Deoxidizing and freezing device |
| CN112795465A (en) * | 2021-03-12 | 2021-05-14 | 北京大学深圳研究生院 | Novel anaerobe liquid medium deoxidization device |
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