CN209927790U - Detection pool for low-flow liquid chromatograph detector - Google Patents

Abstract

The utility model discloses a detection pool for a low-flow liquid chromatograph detector, which has simple structure, convenient processing, low diffusion and high sensitivity, and is provided with a pool body, a square groove is arranged in the center of the pool body, the left side and the right side of the square groove are respectively connected with a liquid inlet pipe and a liquid outlet pipe, the front side and the rear side of the square groove are respectively connected with an optical fiber fixing piece, a pool core is arranged in the square groove and is formed by connecting an upper quartz overflowing block, a lower quartz overflowing block, a front quartz pressing block, a rear quartz pressing block, a front channel and a rear channel, a detection flow path communicated with the front channel and the rear channel is arranged between the upper quartz flow-through block and the lower quartz flow-through block, the front channel is coaxial with the liquid inlet pipe, the rear channel is coaxial with the liquid outlet pipe, the detection flow path is coaxial with the optical fiber fixing piece, and a sealing cover is fixed on the square groove.

Description

Detection pool for low-flow liquid chromatograph detector

Technical Field

The utility model relates to a detection pond of the visible detector of ultraviolet among the high performance liquid chromatography system, especially a detection pond for low flow liquid chromatograph detector of simple structure, processing convenience and low diffusion, sensitivity are high.

Background

The ultraviolet-visible detector is one of the devices of the liquid chromatography system, and the detection cell is the core component of the detector. The existing detection pool is provided with a stainless steel pool body, a Z-shaped liquid flow path and a light path are processed in the stainless steel pool body through a drilling process, the Z-shaped liquid flow path is provided with a liquid inlet and a liquid outlet, a detection flow path is arranged between the liquid inlet and the liquid outlet, and an incident light path and a reflection light path are respectively arranged on two transverse sides of the liquid channel and are coaxial with the liquid channel. When the liquid chromatography system works, the effluent separated by the chromatographic column enters the detection pool through the fluid pipeline and the liquid inlet, and flows out of the liquid outlet after passing through the detection flow path. The monochromatic light split by the detector monochromator is introduced into the detection cell through the incident light path and is absorbed by the liquid part flowing through the detection flow path. The absorbed monochromatic light is led out from the detection cell to a photocell in the detector through a reflection light path, and light intensity information is obtained. And finally, carrying out ratio or logarithm operation on the light intensity of the incident light and the outgoing light to obtain the transmittance or absorbance information of the flowing liquid, and continuously collecting the absorbance information within a period of time to obtain a chromatogram. Factors such as the optical path of the detection cell, the volume of the cell, the flow path structure and the like directly influence the sensitivity of detection signals, and even influence the chromatographic resolution due to the diffusion problem.

The detection cell of the prior ultraviolet-visible detector is generally designed to have an optical path of 10mm and a cell volume of 10 mu L, but is not suitable for low-flow liquid chromatographs such as micro-column liquid chromatography, capillary liquid chromatography, nano-liter liquid chromatography and the like because of overlarge cell volume. Therefore, at present, in laboratories and some commercial instruments, an on-column detection method is mostly adopted, i.e. a part of coating of the capillary is removed to be used as a detection window. Although the volume of the detection pool can be obviously reduced, the detection sensitivity is extremely low because the optical path of detection is only equivalent to the inner diameter of the capillary.

Disclosure of Invention

The utility model relates to a solve the above-mentioned problem that prior art exists, provide a simple structure, processing convenience and low diffusion, sensitivity is high low flow liquid chromatograph detects pond for detector.

The technical solution of the utility model is that: a detection pool for a low-flow liquid chromatograph detector comprises a pool body, wherein a square groove is formed in the center of the pool body, the left side and the right side of the square groove are respectively connected with a liquid inlet pipe and a liquid outlet pipe, the front side and the rear side of the square groove are respectively connected with an optical fiber fixing piece, a pool core is arranged in the square groove and is formed by connecting an upper quartz overflowing block, a lower quartz overflowing block, quartz pressing blocks on the front side and the rear side, a front channel is formed between the upper quartz overflowing block and the lower quartz overflowing block and the quartz pressing blocks on the front side and the rear side, a detection flow path communicated with the front channel and the rear channel is formed between the upper quartz overflowing block and the lower quartz overflowing block, the front channel is coaxial with the liquid inlet pipe, the rear channel is coaxial with the liquid outlet pipe, the detection flow path is coaxial with the optical fiber fixing piece, and a.

The utility model discloses a precision finishing on quartz surface forms the detection flow path of nano-grade volume on the pond core, is applicable to low flow liquid chromatograph detector. Meanwhile, the incident light is guided out by using the optical fiber, so that the detection light can penetrate through the sample in the cell to the maximum extent, the post-column volume of a chromatographic system is obviously reduced, and the diffusion effect of the sample in the cell is reduced; the incident light is guided out by the optical fiber, so that the difficulty of light focusing is reduced, the problem of reduced luminous flux caused by reduction of the cell hole is solved, and the detection sensitivity is improved; in addition, owing to utilize optic fibre to guide out the incident light, the utility model discloses a detection pond can arrange the instrument outside in, realizes remote detection, has promoted the practicality of instrument.

Drawings

Fig. 1 is a structural sectional view of an embodiment of the present invention.

Fig. 2 is a cross-sectional view of a cell core according to an embodiment of the present invention.

Fig. 3 is a schematic structural diagram of a pool core according to an embodiment of the present invention.

Fig. 4 is an overall three-dimensional view of an embodiment of the invention.

Detailed Description

The utility model discloses a low flow liquid chromatograph detector is with detecting pond is shown as picture 1, 2, 3, 4, has cell body 1, and there is square groove 2 at cell body 1 center, the 2 left and right sides of square groove are connected with liquid admission pipe 3, liquid exit tube 4 through screw 14, sword ring 15 respectively, and both sides meet with optic fibre mounting 5 (the optic fibre screw hole that matches with FC type joint) respectively around the square groove 2, are equipped with the pond core in square groove 2, and the pond core is crossed the quartz briquetting 8 of flow block 6, 7 and front and back both sides by upper and lower quartz, 9 and meets and forms, adopts the resin adhesive that can bear ultraviolet light and liquid chromatogram mobile phase to bond each other. A front channel 10 is formed between the upper and lower quartz overflow blocks 6, 7 and the front quartz press block 8, a rear channel 11 is formed between the upper and lower quartz overflow blocks 6, 7 and the rear quartz press block 9, namely, grooves are processed on the left side of the front and the right side of the rear between the upper and lower quartz overflow blocks 6, 7, the front channel 10 and the rear channel 11 are respectively formed with the quartz press blocks 8, 9 on the front and the rear sides, a detection flow path (groove) 12 communicated with the front channel 10 and the rear channel 11 is also processed in the middle part between the upper and lower quartz overflow blocks 6, 7, the front channel 10 is coaxial with the liquid inlet pipe 3, the rear channel 11 is coaxial with the liquid outlet pipe 4, the detection flow path 12 is coaxial with the optical fiber fixing member 5 (namely coaxial with the installed optical fiber), and a sealing cover 13 is fixed on the square groove 2.

The width of the front channel 10, the width of the rear channel 11 and the detection flow path 11 are 0.2 mm, the depth of the front channel 11 is 0.1 mm, the length of the front channel 10 and the length of the rear channel 11 are 4.9 mm, the length of the detection flow path 12 is 2.5 mm, and then the sectional area of the front channel 10 and the sectional area of the rear channel 11 are 0.04 mm²The volume was 196 nL, and the cross-sectional area of the detection channel 12 was 0.04 mm²The volume of the tank is 100 nL.

Claims (1)

1. The utility model provides a low flow liquid chromatograph detects pond for detector, has cell body (1), its characterized in that: the center of the pool body (1) is provided with a square groove (2), the left side and the right side of the square groove (2) are respectively connected with a liquid inlet pipe (3) and a liquid outlet pipe (4), the front side and the rear side of the square groove (2) are respectively connected with an optical fiber fixing piece (5), a pool core is arranged in the square groove (2), the pool core is formed by connecting an upper quartz overflowing block (6), a lower quartz overflowing block (7), quartz pressing blocks (8, 9) at the front side and the rear side, a front channel (10) is formed between the upper quartz overflowing block (6), the lower quartz overflowing block (7) and the front quartz pressing block (8), a rear channel (11) is formed between the upper quartz overflowing block (6) and the lower quartz block (7) and the rear quartz pressing block (9), a detection flow path (12) communicated with the front channel (10) and the rear channel (11) is arranged between the upper quartz overflowing block (6) and the lower quartz block (7), the front channel (10) is coaxial with the liquid inlet pipe (3), and, the detection flow path (12) is coaxial with the optical fiber fixing piece (5), and a sealing cover (13) is fixed on the square groove (2).

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