CN102338787B - UV-visible detector flow-through cell - Google Patents
UV-visible detector flow-through cell Download PDFInfo
- Publication number
- CN102338787B CN102338787B CN201010235405.5A CN201010235405A CN102338787B CN 102338787 B CN102338787 B CN 102338787B CN 201010235405 A CN201010235405 A CN 201010235405A CN 102338787 B CN102338787 B CN 102338787B
- Authority
- CN
- China
- Prior art keywords
- flow
- cell body
- flow cell
- cell
- visible detector
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Abstract
The invention discloses an UV-visible detector flow-through cell, which comprises a flow-through cell body and front and rear clamping plates connected on the flow-through cell body. pads are disposed between the flow-through cell body and the front clamping plate and between the flow-through cell body and the rear clamping plate; the flow-through cell body, the front and rear clamping plates and corresponding position of the pads are provided with location holes, inside which positioning pins are disposed; and quartz glass window on the front and rear clamping plates is provided with pressed nuts through a gasket. According to the UV-visible detector flow-through cell provided by the invention, the pressed nuts are used to tightly press the quartz glass window on the front and rear clamping plates onto the pads through the gasket, so as to enhance compressive strength of the flow-through cell, prevent a liquid from flowing out along the contact surface of the pads and the flow-through cell body and solve the problem of liquid leakage caused by poor compressive strength of a present flow-through cell.
Description
Technical field
The present invention relates to liquid-phase chromatographic analysis technical field, more particularly, relate to a kind of UV-visible detector flow-through cell.
Background technology
UV-visible detector, based on solute molecule, to absorb the detecting device of the principle design of ultraviolet-visible light, when ultraviolet-visible segment beam sees through flow cell, if mobile phase absorbs light, now, its absorbance log is directly proportional to the concentration of extinction component and the optical path length of flow cell.At present, UV-visible detector is most widely used detecting device, it have highly sensitive, noise is low, the range of linearity is wide, to the equal advantage such as insensitive of flow velocity and temperature, can be used for analysis, preparative chromatography and microanalysis.
Wherein: UV-visible detector flow-through cell is one of core component of this detecting device, its Main Function is to provide the passage that a light beam and mobile phase cross, make the lasting very little mobile phase of irradiation pulsation of ultraviolet-visible segment beam, produce light absorption, thereby realize the quantitative test of various samples.
Owing to flowing into the liquid of flow cell, there is high pressure, improve the highest withstand voltage stability that is conducive to improve instrument of flow cell, yet existing flow cell is withstand voltage not, the in the situation that of pressurized behind line clogging or pond, can produce leakage, causes the unstable of instrument.
Summary of the invention
In view of this, the invention provides a kind of UV-visible detector flow-through cell, to solve existing flow cell, because compressive resistance is poor, cause generating the problem of leakage.
For addressing the above problem, the technical scheme of proposition is as follows:
A UV-visible detector flow-through cell, comprising: flow cell body and be connected to the forward and backward clamping plate on described flow cell body; Wherein:
Between described flow cell body and forward and backward clamping plate, be all placed with pad;
On the relevant position of described flow cell body, forward and backward clamping plate and pad, be provided with pilot hole, in hole, be placed with register pin;
On four jiaos of described flow cell body and on the relevant position of front plate, rear plate, offer the threaded hole of placing screw, place screw in threaded hole, fixedly front plate and rear plate are on flow cell body;
On quartz window on described forward and backward clamping plate, by packing ring, gland nut is installed.
Preferably, in described flow cell body, be provided with two flow cells, be respectively reference cell and measuring cell.
Preferably, described reference cell is cylindrical hole, and described measuring cell is round taper hole.
Preferably, described pad is polyimide material pad.
Preferably, described flow cell body is stainless steel flow cell body.
Preferably, the entrance and exit on described flow cell body is connected with respectively stainless-steel pipe.
Preferably, the internal diameter of described stainless-steel pipe is 0.18mm, and external diameter is 1.6mm.
From above-mentioned technical scheme, can find out, in UV-visible detector flow-through cell disclosed by the invention, gland nut is pressed on the quartz window on forward and backward clamping plate on pad by packing ring, strengthened the compressive resistance of flow cell, avoid liquid to flow out along the surface of contact of pad and flow cell body, solved existing flow cell and because compressive resistance is poor, caused generating the problem of leakage.
Accompanying drawing explanation
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, to the accompanying drawing of required use in embodiment or description of the Prior Art be briefly described below, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skills, do not paying under the prerequisite of creative work, can also obtain according to these accompanying drawings other accompanying drawing.
Fig. 1 is the structural representation of the disclosed a kind of UV-visible detector flow-through cell of the embodiment of the present invention;
Fig. 2 is the disclosed a kind of UV-visible detector flow-through cell assembling schematic diagram of the embodiment of the present invention;
Fig. 3 is the D-D cut-open view of Fig. 1;
Fig. 4 is the C-C cut-open view of Fig. 1.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, obviously, described embodiment is only the present invention's part embodiment, rather than whole embodiment.Embodiment based in the present invention, those of ordinary skills, not making the every other embodiment obtaining under creative work prerequisite, belong to the scope of protection of the invention.
The embodiment of the invention discloses a kind of UV-visible detector flow-through cell, to solve existing flow cell, because compressive resistance is poor, cause generating the problem of leakage.
As illustrated in fig. 1 and 2, the disclosed UV-visible detector flow-through cell of the present embodiment comprises: flow cell body 1 and be connected to front plate 2, the rear plate 3 on flow cell body 1; Wherein:
Between flow cell body 1 and front plate 2, rear plate 3, be all placed with pad 4;
On the relevant position of flow cell body 1, front plate 2, rear plate 3 and pad 4, be provided with pilot hole, in hole, be placed with register pin 5;
Quartz window 6 on by packing ring 7, gland nut 8 is installed.
Concrete, offer for placing screw thread hole on four jiaos of flow cell body 1, when assembling flow cell, the position of calibration flow cell body 1, front plate 2 and rear plate 3 upper screwed holes, make one one correspondence, screw is screwed in threaded hole, fixedly front plate 2 and rear plate 3 are on flow cell body 1.
Below introduce in detail the assembling process of this UV-visible detector flow-through cell:
One in pad 4 is placed between flow cell body 1 and front plate 2, another is placed between flow cell body 1 and rear plate 3, and, aim at the position of the pilot hole on flow cell body 1, front plate 2 and rear plate 3, register pin 5 is inserted in pilot hole, successively through flow cell body 1, front plate 2 and rear plate 3; Screw is screwed in to the threaded hole on rear plate 3, flow cell body 1 and front plate 2 successively, fixedly flow cell body 1, front plate 2, rear plate 3 and two pads 4; Finally, gland nut 8 is pressed on quartz window 6 by packing ring 7, like this, between two pads 4 and flow cell body 1, sealing is stronger, strengthens the compressive strength of flow cell, avoids it because the problem of the low generation leakage causing of compressive strength.
In the process of testing, in order to improve the signal to noise ratio (S/N ratio) of detecting device, the flow cell that this embodiment provides adopts diplopore pond, one is reference cell, one is measuring cell, and thus, more trend is consistent to measure the noise variation that is subject to external interference with the signal of reference two-way and produces.
And the design of UV-visible detector flow-through cell must reduce light scattering as far as possible, and, need to be optical length and internal diameter is little, the stream flow cell passage of turbulization not, like this, can make detecting device reach higher stability and sensitivity.
Empirical tests obtains, conical flow cell can be eliminated " liquid prism " effect of the variations in refractive index formation of pond inner fluid, strengthen detecting device stability and sensitivity, so, in the flow cell that the present embodiment provides, measuring cell adopts round taper hole, and its specification is: end diameter 0.9mm, outside diameter 1.4mm, length 10mm, volume 10.5 μ L.Reference cell can adopt cylindrical hole, and its specification is: diameter 1.4mm, length 10mm, volume 15.4 μ L.
As shown in Figure 3, round taper hole measuring cell 11 crosses flow cell body 1, small end contacts with the pad 4 between front plate 2 and flow cell body 1, large end contacts with the pad 4 between rear plate 3 and flow cell body 1, and, in flow cell body 1, be also provided with the entrance 14 and the outlet 16 that are connected with measuring cell 11 two ends, and be connected with respectively stainless-steel tube in entrance 14 and outlet 16, concrete, mobile phase flows into measuring cell 11 through stainless-steel tube 13 by entrance 14, by its small end, to large end, flowed, by outlet 16, flow out measuring cell 11 again, finally by stainless-steel tube 15, flow out flow cell body 1.Equally, as shown in Figure 4, cylindrical hole reference cell 12 also crosses flow cell body 1, two ends contact with pad 4 respectively, and these measuring cell 11 two ends are also connected and are connected with entrance 18 and outlet 20, and are connected with respectively stainless-steel tube in entrance 18 and outlet 20, concrete, mobile phase flows into reference cell 12 through stainless-steel tube 17 by entrance 18, then flows out reference cell 12 by outlet 20, finally by stainless-steel tube 19, flows out flow cell body 1.
Meanwhile, light,, after being partially absorbed by mobile phase then is irradiated to and on photo-electric conversion element, is produced electric signal and measure and process by opposite side quartz window 6 by irradiating flow cell from a side of flow cell.
Concrete, the stainless-steel tube being connected on the entrance and exit of measuring cell 11 and reference cell 12 is annulus column jecket, and its internal diameter can be 0.18mm, and external diameter can be 1.6mm.
At the disclosed UV-visible detector flow-through cell of the embodiment of the present invention, in order to improve the crushing resistance of flow cell body and the crushing resistance of pad, adopt stainless steel to produce flow cell body, adopt the polyimide with high temperature resistant, low temperature resistant, excellent mechanical properties and radiation-resistant property advantage to produce pad.
In sum, in UV-visible detector flow-through cell disclosed by the invention, after location by register pin and screw fixing, gland nut is pressed on the quartz window on forward and backward clamping plate on pad by packing ring again, strengthened the compressive resistance of flow cell, avoid liquid to flow out along the surface of contact of pad and flow cell body, solved existing flow cell and because compressive resistance is poor, caused generating the problem of leakage.
In this instructions, each embodiment adopts the mode of going forward one by one to describe, and each embodiment stresses is the difference with other embodiment, between each embodiment identical similar part mutually referring to.
Above-mentioned explanation to the disclosed embodiments, makes professional and technical personnel in the field can realize or use the present invention.To the multiple modification of these embodiment, will be apparent for those skilled in the art, General Principle as defined herein can, in the situation that not departing from the spirit or scope of the present invention, realize in other embodiments.Therefore, the present invention will can not be restricted to these embodiment shown in this article, but will meet the widest scope consistent with principle disclosed herein and features of novelty.
Claims (7)
1. a UV-visible detector flow-through cell, is characterized in that, comprising: flow cell body and be connected to the forward and backward clamping plate on described flow cell body; Wherein:
Between described flow cell body and forward and backward clamping plate, be all placed with pad;
On the relevant position of described flow cell body, front plate, rear plate and pad, be provided with pilot hole, in hole, be placed with register pin;
On four jiaos of described flow cell body and on the relevant position of front plate, rear plate, offer the threaded hole of placing screw, place screw in threaded hole, fixedly front plate and rear plate are on flow cell body;
On quartz window on described forward and backward clamping plate, by packing ring, gland nut is installed;
Wherein, the assembling process of described UV-visible detector flow-through cell is:
One in pad is placed between flow cell body and front plate, another is placed between flow cell body and rear plate, and, the position of the pilot hole on aligning flow cell body, front plate and rear plate, register pin is inserted in pilot hole, successively through flow cell body, front plate and rear plate; Screw is screwed in to the threaded hole on rear plate, flow cell body and front plate successively, fixedly flow cell body, front plate, rear plate and two pads; Gland nut is pressed on quartz window by packing ring.
2. UV-visible detector flow-through cell according to claim 1, is characterized in that, is provided with two flow cells in described flow cell body, is respectively reference cell and measuring cell.
3. UV-visible detector flow-through cell according to claim 2, is characterized in that, described reference cell is cylindrical hole, and described measuring cell is round taper hole.
4. UV-visible detector flow-through cell according to claim 1, is characterized in that, described pad is polyimide material pad.
5. UV-visible detector flow-through cell according to claim 1, is characterized in that, described flow cell body is stainless steel flow cell body.
6. UV-visible detector flow-through cell according to claim 1, is characterized in that, the entrance and exit on described flow cell body is connected with respectively stainless-steel pipe.
7. UV-visible detector flow-through cell according to claim 6, is characterized in that, the internal diameter of described stainless-steel pipe is 0.18mm, and external diameter is 1.6mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010235405.5A CN102338787B (en) | 2010-07-21 | 2010-07-21 | UV-visible detector flow-through cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010235405.5A CN102338787B (en) | 2010-07-21 | 2010-07-21 | UV-visible detector flow-through cell |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102338787A CN102338787A (en) | 2012-02-01 |
| CN102338787B true CN102338787B (en) | 2014-04-02 |
Family
ID=45514624
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201010235405.5A Active CN102338787B (en) | 2010-07-21 | 2010-07-21 | UV-visible detector flow-through cell |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102338787B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9880090B2 (en) * | 2014-08-15 | 2018-01-30 | Bio-Rad Laboratories, Inc. | HPLC reverse-flow flow cell |
| CN106769861A (en) * | 2016-12-13 | 2017-05-31 | 上海仪电分析仪器有限公司 | A kind of flow cell body structure-improved for strengthening antijamming capability |
| CN106680209A (en) * | 2016-12-13 | 2017-05-17 | 上海仪电分析仪器有限公司 | Flow cell body installation and improvement structure for enhancing sealing performance |
| US11408818B2 (en) * | 2017-03-17 | 2022-08-09 | Shimadzu Corporation | Flow cell unit |
| CN110346461B (en) * | 2018-04-03 | 2022-01-11 | 中国科学院大连化学物理研究所 | Light detection flow cell |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4192614A (en) * | 1975-02-06 | 1980-03-11 | The Perkin-Elmer Corporation | L/C detector cell assembly |
| GB2097548A (en) * | 1981-04-27 | 1982-11-03 | Accuspec Ltd | Flow-through cells for spectroscopy |
| US5054919A (en) * | 1989-02-07 | 1991-10-08 | Linear Instruments Corporation | Seal for high pressure and small volume sample cells |
| CN2689227Y (en) * | 2004-04-08 | 2005-03-30 | 上海伍丰科学仪器有限公司 | Detector flowing pool of liquid-phase chromatograph |
-
2010
- 2010-07-21 CN CN201010235405.5A patent/CN102338787B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4192614A (en) * | 1975-02-06 | 1980-03-11 | The Perkin-Elmer Corporation | L/C detector cell assembly |
| GB2097548A (en) * | 1981-04-27 | 1982-11-03 | Accuspec Ltd | Flow-through cells for spectroscopy |
| US5054919A (en) * | 1989-02-07 | 1991-10-08 | Linear Instruments Corporation | Seal for high pressure and small volume sample cells |
| CN2689227Y (en) * | 2004-04-08 | 2005-03-30 | 上海伍丰科学仪器有限公司 | Detector flowing pool of liquid-phase chromatograph |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102338787A (en) | 2012-02-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102338787B (en) | UV-visible detector flow-through cell | |
| CN100359318C (en) | Ultraviolet-ray visible absorbing/fluorescent dual-purpose flow cell | |
| CN100371710C (en) | Portable liquid-phase chromatograph | |
| CN100504350C (en) | Sandwiched liquid core waveguide structure detection pond | |
| CN103998917A (en) | Flow cell and liquid analyzer | |
| CN104048916B (en) | Flow cell | |
| JP2013507615A (en) | Multi-stream spectrophotometer module | |
| US3619068A (en) | Refractometer | |
| CN102072880A (en) | Large-capacity preparation flow cell with optical fiber interface | |
| CN103760107B (en) | A kind of liquid core waveguide flow cell for ultraviolet spectrometry detector | |
| CN110531013A (en) | A kind of detection cell being axially totally reflected using capillary wall | |
| CN101173883A (en) | On-line weighing type density sensor | |
| US4844611A (en) | Split stream flow cell | |
| CN103207254A (en) | Reflecting ultraviolet-visible absorption and fluorescence integrated flow cell | |
| CN205120679U (en) | Liquid chromatogram ultraviolet and visible light detector total reflection overlength optical distance detects pond | |
| CN101571484B (en) | Nano-material surface-catalyzed luminescent micro-sensor | |
| CN203929616U (en) | A kind of flow cell with optical fiber interface | |
| CN202049109U (en) | Linear-combination gas concentration detecting cell device | |
| CN208654101U (en) | A kind of gas sample injection device measuring stationary source total organic carbon | |
| CN206410998U (en) | The circulation pond body for strengthening sealing property installs structure-improved | |
| CN209927790U (en) | Detection pool for low-flow liquid chromatograph detector | |
| JPH0360386B2 (en) | ||
| CN216847458U (en) | Ultraviolet light UV flow-through cell subassembly | |
| CN201138322Y (en) | Density sensor in on-line weighing type | |
| CN218938216U (en) | Adjustable optical path online flow cell |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |