CN2811989Y - Fluorescent detection pool for micro-liquid phase flow analysis system - Google Patents
Fluorescent detection pool for micro-liquid phase flow analysis system Download PDFInfo
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- CN2811989Y CN2811989Y CN 200520091719 CN200520091719U CN2811989Y CN 2811989 Y CN2811989 Y CN 2811989Y CN 200520091719 CN200520091719 CN 200520091719 CN 200520091719 U CN200520091719 U CN 200520091719U CN 2811989 Y CN2811989 Y CN 2811989Y
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- light path
- liquid phase
- phase flow
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The utility model relates to a fluorescence detecting device, particularly a fluorescent detecting pool for a micro-liquid phase flow analyzing system. An exciting light path, a liquid flow path and a fluorescence collecting light path are arranged on a pool body, wherein the exciting light path and the liquid flow path are positioned in a same plane, and the fluorescence collecting light path is perpendicular to the plane. The central lines of the exciting light path, the liquid flow path and the fluorescence collecting light path are perpendicular to each other and are in conjugated cross; the liquid flow path forms a through-hole structure; the pool body is made of metallic materials or made of nonmetallic materials, such as polyether ether ketone or teflon, etc.; the fluorescent detecting pool for a micro-liquid phase flow analyzing system is coupled with optical parts through optical fibers. The fluorescent detecting pool for a micro-liquid phase flow analyzing system of the utility model is coupled with other optical parts through the optical fibers, therefore the utility model can realize the autocollimation of a light path without optical calibration, and has the advantages of low optical background and small extra-column effect.
Description
Technical field
The utility model relates to fluorescence detection device, particularly a kind of little liquid phase flow analytic system fluorescence detection cell.
Background technology
Fluoroscopic examination has high sensitivity and strong selectivity as a kind of photoluminescence type detection technique, uses very extensive in trace samplings detects.As the vitals of fluorescence detecting system, the quality of detection cell performance has directly determined the quality of overall performance.The characteristics that good detection cell should have are: parasitic light is little, the optics background is low, lasing efficiency and phosphor collection efficient height and miscellaneous part are easy to connect.
Chromatographic theory shows that extra column effect is to influence the key factor that post is imitated, and column internal diameter is more little, requires strict more to extra column effect.For reducing the detection cell band spread, Frank Yang etc. has introduced on the post and has detected.This pattern has reduced the post external diffusion to greatest extent, makes also simple relatively.In little liquid phase flow analytic system, use very general.But because factors such as the cross-sectional area of kapillary column type and light path weak points, therefore the shortcoming of bringing is that optics background height, detection sensitivity are low.In addition, the capillary detection pond of micron order size has also increased optical correction's difficulty.For reducing parasitic light and calibration difficulties, Gerald L.Klein etc. has designed a kind of windowless detection cell, promptly in two holes of making a call to of kapillary radial position symmetry, two optical fiber is placed on two holes, with glue optical fiber and kapillary are sticked together, optical fiber directly contacts with moving phase.Although this design can address the above problem, the not a duck soup that punches on tens microns the capillary wall is only arranged at diameter.
The utility model content
The purpose of this utility model is to provide a kind of little liquid phase flow analytic system fluorescence detection cell of realizing light path automatically collimating.This detection cell is applicable to little liquid phase flow analytic systems such as Capillary Electrophoresis, capillary liquid chromatography and flow injections.
The purpose of this utility model is achieved through the following technical solutions:
Pond of the present utility model body is provided with excitation light path, liquid flow path and phosphor collection light path, and wherein excitation light path and liquid flow path are in the same plane, and the phosphor collection light path is vertical with this plane.
Wherein: described excitation light path, liquid flow path and phosphor collection light path three's center line is vertically gripped intersection mutually altogether; Described liquid flow path is a through-hole structure; Described pond body is processed by non-metallic materials such as metal material or polyetheretherketone or Teflons; Described detection cell is by optical fiber and optics coupling.
Advantage of the present utility model and good effect are:
The utility model with detection cell and other optics couplings, can be realized the autocollimation of light path by optical fiber, need not optical correction, and the optics background is low, and extra column effect is little.
Description of drawings
Fig. 1 is a structural representation of the present utility model;
Fig. 2 is the distribution schematic diagram of the utility model stream and light path;
Fig. 3 is used for the analysis of spectra of Flow Injection Analysis system for the utility model;
Fig. 4 is used for the chromatogram of micro column liquid chromatography system for the utility model.
Embodiment
The utility model is described in further detail below in conjunction with accompanying drawing.
As shown in Figure 1 and Figure 2, pond of the present utility model body 4 is provided with excitation light path 1, liquid flow path 2 and phosphor collection light path 3, wherein excitation light path 1 is in the same plane with liquid flow path 2, and excitation light path 1, liquid flow path 2 and phosphor collection light path 3 threes' center line is vertically gripped intersection mutually altogether.Liquid flow path 2 is a through-hole structure, avoids light signal to be subjected to fluid stream deformation disturbance.Pond body 4 is processed by metal material or polyetheretherketone (PEEK) or Teflon non-metallic materials such as (Teflon).Detection cell is by optical fiber and other optics couplings.
During use, optical fiber is inserted by excitation light path 1, phosphor collection light path 3, and at the identical concentric plastic casing of the termination point casing top external diameter of optical fiber, and be fixed on the detection cell with nut and cutting ferrule.The exciting light scioptics focus on the optical fiber (excitation fiber) in the excitation light path 1, the sample in the liquid flow path 2 be stimulated the fluorescence that produces be transferred to by the vertical optical fiber of placing (collection optical fiber) in the phosphor collection light path 3 that electrooptical device changes into can be for the electric signal that detects.
As shown in Figure 3, detection cell is used for the test condition of Flow Injection Analysis system and is: carrier fluid: water; Connecting pipe: quartz capillary (0.32mm i.d.); Sample: fluorescein; Sample size: 10 μ L.
As shown in Figure 4, detection cell is used for the test condition of micro column liquid chromatography system and is: separating column is 1 * 150mm C
18, 5 μ, (ZORBAX, HP); Moving phase: water/methyl alcohol (70/30) moving phase: water; Sample: VB
2
The utility model utilizes the orthogonal fixture-detection cell of through hole center line and uses the identical concentric plastic casing of external diameter to be enclosed within on the optical fiber, realizes the autocollimation of light path.
Claims (5)
1. little liquid phase flow analytic system fluorescence detection cell, it is characterized in that: pond body (4) is provided with excitation light path (1), liquid flow path (2) and phosphor collection light path (3), wherein excitation light path (1) is in the same plane with liquid flow path (2), and phosphor collection light path (3) is vertical with this plane.
2. by the described a kind of little liquid phase flow analytic system fluorescence detection cell of claim 1, it is characterized in that: described excitation light path (1), liquid flow path (2) and phosphor collection light path (3) three's center line is vertically gripped intersection mutually altogether.
3. by claim 1 or 2 described a kind of little liquid phase flow analytic system fluorescence detection cells, it is characterized in that: described liquid flow path (2) is a through-hole structure.
4. by the described a kind of little liquid phase flow analytic system fluorescence detection cell of claim 1, it is characterized in that: described pond body (4) is processed by non-metallic materials such as metal material or polyetheretherketone or Teflons.
5. by the described a kind of little liquid phase flow analytic system fluorescence detection cell of claim 1, it is characterized in that: described detection cell is by optical fiber and optics coupling.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520091719 CN2811989Y (en) | 2005-07-08 | 2005-07-08 | Fluorescent detection pool for micro-liquid phase flow analysis system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200520091719 CN2811989Y (en) | 2005-07-08 | 2005-07-08 | Fluorescent detection pool for micro-liquid phase flow analysis system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2811989Y true CN2811989Y (en) | 2006-08-30 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200520091719 Expired - Fee Related CN2811989Y (en) | 2005-07-08 | 2005-07-08 | Fluorescent detection pool for micro-liquid phase flow analysis system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2811989Y (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101614652B (en) * | 2008-06-25 | 2011-04-20 | 中国科学院大连化学物理研究所 | Self-aligned optical-fiber fluorescence detection cell and array fluorescence detection cell |
| CN103389269A (en) * | 2013-07-25 | 2013-11-13 | 浙江清华长三角研究院 | Multifunctional optical detection flow cell device |
| CN104749140A (en) * | 2013-12-25 | 2015-07-01 | 中国科学院大连化学物理研究所 | Flow-through fluorescence detection cell |
| CN109870411A (en) * | 2017-12-04 | 2019-06-11 | 中国科学院大连化学物理研究所 | A kind of adjustable calibrating --- refractive power compensates fluorescence detection cell on column |
-
2005
- 2005-07-08 CN CN 200520091719 patent/CN2811989Y/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101614652B (en) * | 2008-06-25 | 2011-04-20 | 中国科学院大连化学物理研究所 | Self-aligned optical-fiber fluorescence detection cell and array fluorescence detection cell |
| CN103389269A (en) * | 2013-07-25 | 2013-11-13 | 浙江清华长三角研究院 | Multifunctional optical detection flow cell device |
| CN104749140A (en) * | 2013-12-25 | 2015-07-01 | 中国科学院大连化学物理研究所 | Flow-through fluorescence detection cell |
| CN104749140B (en) * | 2013-12-25 | 2017-06-16 | 中国科学院大连化学物理研究所 | A kind of flow type fluorescence detection cell |
| CN109870411A (en) * | 2017-12-04 | 2019-06-11 | 中国科学院大连化学物理研究所 | A kind of adjustable calibrating --- refractive power compensates fluorescence detection cell on column |
| CN109870411B (en) * | 2017-12-04 | 2021-07-06 | 中国科学院大连化学物理研究所 | Adjustable calibration refraction compensation column fluorescence detection pool |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20060830 |