CN209854151U - Culture apparatus suitable for on-spot fungus crowd detects in proruption public health incident - Google Patents
Culture apparatus suitable for on-spot fungus crowd detects in proruption public health incident Download PDFInfo
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- CN209854151U CN209854151U CN201920176564.9U CN201920176564U CN209854151U CN 209854151 U CN209854151 U CN 209854151U CN 201920176564 U CN201920176564 U CN 201920176564U CN 209854151 U CN209854151 U CN 209854151U
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Abstract
The disclosure relates to the technical field of flora culture devices, in particular to a culture device suitable for field flora detection in sudden public health events. In the prior art, the Duhan's tube is used as a culture device for detecting the number of coliform groups in a sample by an MPN method, the exhaust difficulty of the culture tube is higher, and the early-stage preparation workload is greatly increased. In addition, the currently used MPN method is only suitable for being carried out under laboratory conditions, and is not always applied to field detection under emergency situations. In view of the above, the present disclosure provides a ready-to-use culture apparatus and a constant temperature incubator adapted to the culture apparatus. The flexible culture tube and the constant temperature culture device in the disclosure have the advantages of small volume and easy carrying, and the constant temperature culture device with the power supply can detect samples on site under emergency conditions without sending the samples back to a laboratory for detection, thereby effectively shortening rescue time.
Description
Technical Field
The disclosure relates to the technical field of flora culture devices, in particular to a device which is suitable for culturing and detecting flora in field samples in time under the condition of emergent public health events.
Background
The information in this background section is only for enhancement of understanding of the general background of the disclosure and is not necessarily to be construed as an admission or any form of suggestion that this information forms the prior art that is already known to a person of ordinary skill in the art.
The multitube fermentation Most Probable Number (MPN) method is used as a common method for detecting coliform group, heat-resistant coliform group (faecal coliform group) and Escherichia coli in sanitary microorganism indexes of public products such as water, food, cosmetics and public places, and has the advantages of low technical difficulty, low reagent cost, wide detection range and the like. In the national GB/T5750.12-2006 Standard test method for domestic Drinking Water microbial indicators, the test of Total coliform, Heat-resistant coliform and Escherichia coli, the test of Escherichia coli count in GB4789.3-2016 food safety national Standard food microbiology, the test of Escherichia coli count in GB 4789.38-2012 food safety national Standard food microbiology, the test of Escherichia coli count in GB/T18204.10-2000 swimming pool Water microbial test method, and the test of Multi-tube fermentation Most Probable Number (MPN) methods, such as the test of Total coliform in Water and the test of fecal coliform in Water and wastewater monitoring and analysis method (fourth edition), are the first methods; in the inspection of faecal coliform in technical specifications for cosmetic safety (2015 edition), the inspection of faecal coliform population in GB 4789.39-2013 food safety national standard food microbiology, and the inspection of hygiene in GB/T18204.4-2013 public places fourth part: a multi-tube fermentation method of coliform group in public appliance microorganism, namely, a multi-tube fermentation Most Probable Number (MPN) method in an inspection method of fecal coliform group in sewage and sludge in medical institution, namely, appendix A (normative appendix) of GB-18466 and 2005 medical institution water pollutant discharge standard of medical institution, and the like are only methods.
In the multi-tube fermentation Most Probable Number (MPN) method detection process, samples are required to be configured into a series of samples with concentration, and after the samples are cultured in an incubator for a period of time, the approximate value of the number of bacteria in the samples is calculated by adopting a maximum probable number theory according to the lowest dilution without growth and the highest dilution with growth, so that the method can be well suitable for detection of microorganisms in soil, sewage, milk and food. Because the MPN method is convenient to apply, the number of bacteria in a sample can be obtained by comparing the minimum dilution and the maximum dilution with data in an attached table after the minimum dilution and the maximum dilution are obtained, and the MPN method has certain superiority when applied to emergency detection in certain accidents and also has certain limitation: in the detection process of the MPN method, a sample is added into a culture tube and then needs to be cultured in an incubator for about 24 hours, and 1-30 fermentation tubes need to be cultured simultaneously in one sample detection, so that the requirement on the volume of the incubator is large, and the detection process can only be carried out in a laboratory. In addition, the culture tube used in the MPN method detection at present is a Duhan culture tube, an inverted small tube is arranged in the culture tube, air in the inverted small tube needs to be evacuated before use, dozens of culture tubes need to be prepared in advance to complete one-time detection, and the progress of the test is slowed down.
In addition, the inventor also finds that in some emergency situations, such as emergency environments caused by unexpected factors such as earthquakes, floods, microbial sample leakage, improper hazardous waste treatment and the like or irresistible natural disasters and the like, detection personnel need to monitor the microbial content in samples such as soil, water quality, food, public places, public supplies and the like so as to prevent further expansion of disasters, the field environment of the emergency situations is often severe, electric power is difficult to guarantee, laboratories near the disaster field are difficult to normally operate, and in many emergency situations, traffic is easy to block, the detection personnel are not allowed to send samples back to the laboratories for detection, and rapid detection needs to be completed on the field.
SUMMERY OF THE UTILITY MODEL
Based on the current situation, the MPN method culture apparatus suitable for the on-site rapid detection of the emergency situation is provided, the sample detection time can be effectively shortened, and the rescue progress is accelerated.
In view of the above technical problems, the present disclosure provides a culture apparatus capable of detecting the content of macro-organisms in a sample based on the MPN method in a non-laboratory environment. The culture device comprises a ready-to-use culture tube and an adaptive constant temperature incubator, wherein the ready-to-use culture tube comprises a gas production collecting tube and a flexible main tube, and the gas production collecting tube is not arranged in a fermentation tube any more and is not limited by the built-in tube diameter and the inverted position any more. The flexible main pipe can be bent, so that the inner diameter and the inversion angle of the gas production collecting pipe can be increased in degree of freedom, the pipeline connection between the flexible main pipe for fermentation and the gas production collecting pipe is smoother, the gas can be discharged conveniently, and the bottleneck problem of a small pipe inversion and exhaust link in the prior art is solved. In addition, this disclosure still provides a constant temperature culture apparatus of this flexible culture tube of adaptation, and the device utilizes the peltier effect to realize temperature control, has effectually reduced the volume of constant temperature culture module to can effectively optimize power availability factor, make this culture apparatus can be convenient carry to field usage, accomplish constant temperature culture in 18-48 h.
In order to achieve the above technical effects, the present disclosure provides the following technical solutions:
in a first aspect of the present disclosure, a culture device suitable for field flora detection in an emergency public health event is provided, the culture device comprises a culture detection tube and a constant temperature incubator adapted to the culture detection tube, the culture detection tube comprises: the device comprises a main pipe, a gas production collecting pipe and a pipe cap for sealing the main pipe; the main pipe is of a flexible pipe structure and is connected with the gas production collecting pipe; the main pipe, the gas production collecting pipe and the pipe cover form a closed cavity; the main pipe is longer than the gas production collecting pipe, and the inner diameter of the main pipe is larger than the inner diameter of the gas production collecting pipe;
the culture detection tube is also provided with a culture medium;
preferably, the culture medium is one of lactose peptone culture medium, lactose cholate fermentation culture medium, brilliant green lactose cholate broth, lauryl sulfate tryptone broth, lactose broth, crypto broth, EC-MUG culture medium and LST-MUG;
further, when the liquid volume of the culture medium is 5-20 ml, selecting a main pipe with the inner diameter of 0.8-1.5cm and the length of 3-15cm and a gas production collecting pipe with the inner diameter of 0.5-1.2cm and the length of 2-5 cm;
further, when the amount of the culture medium liquid is 50-200ml, a main pipe with the inner diameter of 2.5-4cm and the length of 10-25cm, and a gas production collecting pipe with the inner diameter of 2-3.5cm and the length of 3-10cm are selected.
The constant-temperature incubator comprises a cover body, a display screen, a shell, a constant-temperature incubation module, a control module, a temperature regulation module and a power supply, wherein the cover body and the display screen are arranged at the top of the shell; the constant-temperature culture module, the control module, the temperature regulation module and the power supply are positioned on the inner side of the shell;
the constant-temperature culture module is positioned below the cover body, and the control module, the temperature regulation module and the power supply are positioned below the display screen; a temperature adjusting module is arranged below the control module, and a power supply is arranged below the temperature adjusting module;
the constant-temperature culture module comprises a metal block matched with the detection tube, a temperature sensor and a semiconductor refrigerating sheet, wherein the top end of the metal block is provided with a groove matched with the culture tube, so that the detection tube is placed in the groove and is bent at a certain angle; the side surface of the metal block is provided with a concave hole matched with the temperature sensor.
The temperature sensor is a contact temperature sensor and is provided with a contact part, and the contact part is arranged in the metal block and transmits a temperature signal of the metal block to the temperature adjusting module; the semiconductor refrigerating sheet is connected with the bottom of the metal block in a manner of clinging to the bottom;
the control module comprises a micro-control chip, a memory and a memory;
the temperature adjusting module comprises an AD chip and an AD sampling circuit;
the power supply is a storage battery and provides power for the operation of the constant-temperature incubator.
The incubator in the present disclosure uses a built-in storage battery to provide an energy source for the incubation device, so that the device can be normally used even in the outdoor or other conditions without a power supply. The user sets for cultivateing temperature and time through the control key, and control module cultivates the module to constant temperature with the signal conduction that the technical staff set for and realizes heating and refrigeration through the semiconductor refrigeration piece, and temperature sensor detects metal block temperature feedback to temperature regulation module, if overheated then cools down the metal block through the semiconductor refrigeration piece, if the temperature is low excessively, makes the semiconductor realize heating through current direction in the change semiconductor, and final invariable set temperature heats the culture tube.
Preferably, the display screen comprises a TFT liquid crystal screen and control keys.
Preferably, the cover body is a stainless steel cover with an insulating layer adhered to the inner side.
The lid is located the culture tube top, during the use, opens the lid, arranges the culture tube in the recess of aluminium block, and after the adjustment back position, the lid is covered and is cultivateed.
Preferably, the metal block is an aluminum block.
The aluminum block is used as a constant-temperature culture metal block, the material is light, the temperature rise speed is high, the response to temperature is sensitive, the consumption condition of electric energy in the device can be reduced, and the incubator can be suitable for the detection condition under longer time or severe weather.
Preferably, the temperature sensor is a contact temperature sensor Pt100 platinum resistor.
In general, in the culture process, the temperature error of a culture medium needs to be controlled within 0.1-0.3 ℃, the platinum resistor has good sensitivity when being applied to temperature detection of the device in the disclosure, and in addition, the platinum resistor also has good stability including good physical shock resistance, so that the platinum resistor is more suitable for detection in a sudden environment.
Preferably, the semiconductor refrigerating piece is an XLT2389 type semiconductor refrigerating piece produced by Marlow corporation.
Under the general condition, the culture temperature of the flora is about 37 ℃, and the semiconductor refrigeration sheet adopting the type has good response effect in a medium and low temperature area, and meets the culture temperature requirement of most microorganisms.
Preferably, the AD chip controls the sampling circuit to calculate the temperature value by adopting a linear interpolation method, and the four-wire system connection constant current source excitation method is used for measuring the temperature.
Preferably, the micro-control chip is a 32-bit AMR micro-control chip S3C 2410.
Preferably, the memory is 64M NANDFLASH of K9F1208 UDM.
Preferably, the memory is an extended memory, and 32-bit 64M is formed by adopting SDRAM with model No. HY57V561620 manufactured by MICRON.
Preferably, the shell is made of stainless steel materials pasted with heat insulation layers.
Advantageous effects of the disclosure
1. The culture device comprises a culture tube, a main tube and a gas production collecting tube, wherein the culture tube is provided with a flexible main tube, the angle between the main tube and the gas production collecting tube can be flexibly set, the whole gas production collecting tube and the main tube are completely vertically connected after a liquid sample is added, a bending angle is avoided, namely, a channel in the tube is completely vertically communicated, gas in the tube is easily upwards discharged, and the difficulty of gas discharge is greatly reduced. Aiming at the problem that the detection method in the prior art is difficult to be applied to field detection in emergency, the constant temperature incubator adaptive to the flexible culture tube is provided, the flexible culture tube is easy to carry, the constant temperature incubator has the characteristics of small volume, convenience in carrying, high detection precision and good stability, and can meet the detection of the content of microorganisms in samples such as water quality, soil, food, public places, public articles and the like in disaster fields in emergency.
Drawings
The accompanying drawings, which are included to provide a further understanding of the disclosure, illustrate embodiments of the disclosure and together with the description serve to explain the disclosure and are not to limit the disclosure.
FIG. 1 is a schematic view of a ready-to-use culture apparatus in example 5;
wherein, 1 is a flexible main pipe, 2 is a gas production collecting pipe, 3 is a culture medium, 4 is a pipe cap, and 5 is a sealing cap.
FIG. 2 is a schematic view of a ready-to-use culture apparatus in example 6;
wherein, 1 is a flexible main pipe, 2 is a gas production collecting pipe, 3 is a culture medium, 4 is a pipe cap, and 5 is a sealing cap.
FIG. 3 is a schematic view of a ready-to-use culture apparatus in example 8;
wherein, 1 is a flexible main pipe, 2 is a gas production collecting pipe, 3 is a culture medium, 4 is a pipe cap, and 5 is a sealing cap.
FIG. 4 shows a constant temperature incubator in example 9.
A1 is a cover body, A2 is a shell, A3 is a constant-temperature culture module, 301 is a metal block, 302 is a semiconductor refrigeration piece, 303 is a temperature sensor, 301a is a groove, A4 is a control module, 401 is a micro-control chip, 402 is a storage, 403 is an internal memory, A5 is a temperature regulation module, 501 is an AD chip, 502 is an AD sampling circuit, A6 is a power supply, and A7 is a display screen.
Detailed Description
It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present disclosure. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof, unless the context clearly indicates otherwise.
As introduced in the background art, in the prior art, a duhan's tube is used as a culture device for detecting the number of coliform groups in a sample by an MPN method, and before the culture tube is used, air in a built-in small inverted tube needs to be exhausted completely, so that the exhaust difficulty is high; moreover, dozens of culture tubes are needed for completing one-time sample detection by applying the MPN method, and the adoption of the Duhan culture tubes greatly increases the early-stage preparation workload. In addition, the currently used MPN method is only suitable for being carried out under laboratory conditions, and is not always applied to field detection under emergency situations.
In order to solve the above technical problems, the present disclosure proposes a ready-to-use culture apparatus and a constant temperature incubator using the same.
In order to make the technical solutions of the present disclosure more clearly understood by those skilled in the art, the technical solutions of the present disclosure will be described in detail below with reference to specific examples and comparative examples.
Example 1
A culture detection tube is shown in figure 1 and comprises a flexible main tube 1, a gas production collection tube 2 and a tube cap 4 for sealing the main tube; the flexible main pipe 1 is of a flexible pipe structure capable of being bent, the gas production collecting pipe 2 is of a tubular structure with one closed end, and the flexible main pipe 1 is connected with the gas production collecting pipe 2 in a nested manner; the flexible main pipe 1, the gas production collecting pipe 2 and the pipe cap 4 form a closed cavity.
The flexible main pipe 1 is: the silicone rubber straight tube with the inner diameter of 1.1cm and the length of 15cm and two open ends has the Shore hardness of 60 degrees and the elastic energy of more than 45 degrees.
The gas production collecting pipe 2 comprises: a transparent PP plastic straight pipe with the inner diameter of 0.8cm, the length of 3cm, an opening at one end and a sealed round bottom at one end.
The pipe cap 4 is: selecting a plastic test tube cap matched with a 1.1cm inner diameter tube.
Example 2
A culture detection tube, as shown in figure 2, comprises a flexible main tube 1, a gas production collecting tube 2 and a tube cap 4 for sealing the main tube; the flexible main pipe 1 is of a flexible pipe structure capable of being bent, the gas production collecting pipe 2 is of a tubular structure with one closed end, and the flexible main pipe 1 is connected with the gas production collecting pipe 2 in a nested manner; the flexible main pipe 1, the gas production collecting pipe 2 and the pipe cap 4 form a closed cavity.
The flexible main pipe 1 is: the transparent organic silica gel straight tube with the inner diameter of 1.2cm and the length of 25cm and two open ends has the Shore hardness of 60 degrees and the elastic energy of more than 45 degrees.
The gas production collecting pipe 2 comprises: a transparent PP plastic straight pipe with the inner diameter of 1.0cm, the length of 3cm, an opening at one end and a sealed round bottom at one end.
The pipe cap 4 is: selecting a breathable organic silica gel test tube cap matched with a 1.2cm inner diameter tube.
Example 3
A culture detection tube is shown in figure 1 and comprises a flexible main tube 1, a gas production collection tube 2 and a tube cap 4 for sealing the main tube; the flexible main pipe 1 is of a flexible pipe structure capable of being bent, the gas production collecting pipe 2 is of a tubular structure with one closed end, and the flexible main pipe 1 is connected with the gas production collecting pipe 2 in a nested manner; the flexible main pipe 1, the gas production collecting pipe 2 and the pipe cap 4 form a closed cavity.
The flexible main pipe 1 is: the silicone rubber straight pipe with the inner diameter of 3.2cm and the length of 20cm and two open ends has the Shore hardness of 60 degrees and the elastic energy of more than 45 degrees.
The gas production collecting pipe 2 comprises: a transparent PP plastic straight pipe with the inner diameter of 3.0cm, the length of 3cm, an opening at one end and a sealed round bottom at one end.
The pipe cap 4 is: selecting a breathable organic silica gel test tube cap matched with a 3.2cm inner diameter tube.
Example 4
A culture detection tube, as shown in figure 3, comprises a flexible main tube 1, a gas production collecting tube 2 and a tube cap 4 for closing the main tube; the flexible main pipe 1 is of a flexible pipe structure capable of being bent, the gas production collecting pipe 2 is of a tubular structure with one closed end, and the flexible main pipe 1 is connected with the gas production collecting pipe 2 in a nested manner; the flexible main pipe 1, the gas production collecting pipe 2 and the pipe cap 4 form a closed cavity.
The flexible main pipe 1 is: the transparent organic silica gel straight tube with the inner diameter of 1.5cm and the length of 20cm and two open ends has the Shore hardness of 60 degrees and the elastic energy of more than 45 degrees.
The gas production collecting pipe 2 comprises: a transparent PP plastic straight pipe with the inner diameter of 1.0cm, the length of 5cm, an opening at one end and a sealed round bottom at one end.
The pipe cap 4 is: selecting a breathable organic silica gel test tube cap matched with a 1.5cm inner diameter tube.
The gas production collecting pipe 2 comprises: one end is closed by a sealing cap 5.
Example 5
This embodiment provides a culture apparatus suitable for field flora detection in a public health emergency, as shown in fig. 1, the culture apparatus includes: a culture detector tube as described in example 1 and medium 3, said medium 3 being located in said culture detector tube.
The culture medium 3 is: lactose peptone medium, dry powder type was used.
The using method comprises the following steps: when detecting a sample, preparing the sample into liquid with appropriate dilution according to corresponding standards or methods, directly adding the liquid into the ready-to-use culture device for the most probable number detection in the embodiment, and uniformly mixing; then the flexible main pipe 1 is bent by a certain angle, the tail end of the interface of the flexible main pipe 1 and the gas production collecting pipe 2 and the tail end of the interface of the gas production collecting pipe 2 and the flexible main pipe 1 are both inclined upwards by a certain angle, the ready-to-use culture device is placed on a matched bracket, and the tail end of the flexible main pipe 1 connected with the gas production collecting pipe 2 is inclined upwards to keep the fermented liquid to fill the pipeline downwards, so that air is prevented from entering; and the gas generated in the culture can rise to the tail end of the gas production collecting pipe 2, and the culture box is placed in a preset culture condition for culture and then the acid and gas production conditions are observed.
Example 6
A ready-to-use culture device for the most probable detection, as shown in fig. 2, comprising: the culture detector tube and culture medium described in example 2, the culture medium being in the culture detector tube.
The culture medium is as follows: lauryl sulfate tryptone broth (LST).
The procedure was as in example 5.
Example 7
A ready-to-use culture device for the most probable detection, as shown in fig. 1, comprising: the culture detector tube and culture medium described in example 3, the culture medium being in the culture detector tube.
The culture medium is as follows: lactose peptone medium, dry powder type was used.
The procedure was as in example 4.
Example 8
A ready-to-use culture device for the most probable detection, as shown in fig. 3, comprising: the culture detector tube and culture medium described in example 4, the culture medium being in the culture detector tube.
The culture medium is as follows: lactose broth.
The procedure was as in example 5.
Example 9
The constant-temperature incubator comprises a cover body A1, a display screen A7, a shell A2, a constant-temperature incubation module A3, a control module A4, a temperature adjusting module A5 and a power supply A6, wherein the cover body A1 and the display screen A7 are arranged at the top of the shell A2; the constant-temperature culture module A3, the control module A4, the temperature regulation module A5 and the power supply A6 are positioned on the inner side of the shell A2;
the constant-temperature culture module A3 is positioned below the cover A1, and the control module A4, the temperature regulation module A5 and the power supply A6 are positioned below the display screen A7; a temperature adjusting module A5 is arranged below the control module A4, and a power supply A6 is arranged below the temperature adjusting module A5;
the constant-temperature culture module A3 comprises a metal block 301 matched with the detection tube, a temperature sensor 303 and a semiconductor refrigerating sheet 302, wherein the top end of the metal block 301 is provided with a groove 301a matched with the culture tube, so that the detection tube is placed in the groove 301a and is bent at a certain angle; the metal block 301 has a concave hole on its side surface for fitting the temperature sensor 303.
The temperature sensor 303 is a contact temperature sensor and has a contact part, the contact part is embedded in the metal block 301, and the temperature signal of the metal block 301 is transmitted to the temperature adjusting module 5; the semiconductor refrigerating sheet 302 is tightly attached to the bottom of the metal block 301;
the control module A4 comprises a micro control chip 401, a memory 402 and a memory 403;
the temperature adjusting module A5 comprises an AD chip 501 and an AD sampling circuit 502;
the power supply A6 is a storage battery.
The display screen A7 comprises a TFT liquid crystal screen and control keys.
The cover body A1 is a stainless steel cover with an insulating layer adhered to the inner side.
The metal block 301 is an aluminum block.
The temperature sensor 303 is a contact temperature sensor Pt100 platinum resistor.
The semiconductor refrigerating piece 302 is an XLT2389 type semiconductor refrigerating piece produced by Marlow corporation.
The AD chip 501 controls the sampling circuit to calculate the temperature value by adopting a linear interpolation method, and the temperature is measured by a four-wire system connection constant current source excitation method.
The micro-control chip 401 is a 32-bit AMR micro-control chip S3C 2410;
the memory 402 is 64M NANDFLASH of K9F1208UDM
The memory 403 is an extended memory, and 32-bit 64M is formed by adopting an SDRAM with the model number of HY57V561620 manufactured by MICRON.
The shell A2 is made of stainless steel material with an insulating layer.
Example 10
In the constant temperature incubator adapted to the culture tube in the above embodiment, the temperature sensor 303 is a contact type temperature sensor Pt100 platinum resistor.
The semiconductor refrigerating piece 302 is an XLT2389 type semiconductor refrigerating piece produced by Marlow corporation. The AD chip 501 controls the sampling circuit to calculate the temperature value by adopting a linear interpolation method, and the temperature is measured by a four-wire system connection constant current source excitation method.
The micro-control chip 401 is a 32-bit AMR micro-control chip S3C 2410;
the memory 402 is 64M NANDFLASH of K9F1208UDM
The memory 403 is an extended memory, and 32-bit 64M is formed by adopting an SDRAM with the model number of HY57V561620 manufactured by MICRON.
The shell A2 is made of stainless steel material with an insulating layer.
The rest of the setup was the same as in example 9.
The above description is only a preferred embodiment of the present disclosure and is not intended to limit the present disclosure, and various modifications and changes may be made to the present disclosure by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present disclosure should be included in the protection scope of the present disclosure.
Claims (10)
1. The culture device is suitable for field flora detection in sudden public health events and comprises a culture detection tube and a constant-temperature incubator matched with the culture detection tube, wherein the culture detection tube comprises a first culture tube body and a second culture tube body, and the constant-temperature incubator comprises a first culture tube body and a second culture tube body, wherein the first culture tube body is provided with a first culture tube, the second culture tube body is:
the culture detection tube comprises: the device comprises a main pipe, a gas production collecting pipe and a pipe cap for sealing the main pipe; the main pipe is of a flexible pipe structure which can be bent and is connected with the gas production collecting pipe; the main pipe, the gas production collecting pipe and the pipe cover form a closed cavity; the main pipe is longer than the gas production collecting pipe, and the inner diameter of the main pipe is larger than the inner diameter of the gas production collecting pipe;
the constant-temperature incubator comprises a cover body, a display screen, a shell, a constant-temperature incubation module, a control module, a temperature regulation module and a power supply, wherein the cover body and the display screen are arranged at the top of the shell; the constant-temperature culture module, the control module, the temperature regulation module and the power supply are positioned on the inner side of the shell;
the constant-temperature culture module is positioned below the cover body, and the control module, the temperature regulation module and the power supply are positioned below the display screen; a temperature adjusting module is arranged below the control module, and a power supply is arranged below the temperature adjusting module; the constant-temperature culture module comprises a metal block matched with the detection tube, a temperature sensor and a semiconductor refrigerating sheet, wherein a groove matched with the culture tube is formed in the top end of the metal block, so that the detection tube is placed in the groove and is bent at a certain angle; the side surface of the metal block is provided with a concave hole matched with the temperature sensor;
the temperature sensor is a contact temperature sensor and is provided with a contact part, and the contact part is arranged in the metal block and transmits a temperature signal of the metal block to the temperature adjusting module; the semiconductor refrigerating sheet is connected with the bottom of the metal block in a manner of clinging to the bottom;
the control module comprises a micro-control chip, a memory and a memory; the temperature adjusting module comprises an AD chip and an AD sampling circuit; the power supply is a storage battery.
2. The culture device suitable for the field flora detection in sudden public health events according to claim 1, wherein the culture detection tube further comprises a culture medium; preferably, the culture medium is one of lactose peptone culture medium, lactose cholate fermentation culture medium, brilliant green lactose cholate broth, lauryl sulfate tryptone broth, lactose broth, crypto broth, EC-MUG culture medium and LST-MUG;
further, when the liquid volume of the culture medium is 5-20 ml, selecting a main pipe with the inner diameter of 0.8-1.5cm and the length of 3-15cm and a gas production collecting pipe with the inner diameter of 0.5-1.2cm and the length of 2-5 cm;
further, when the amount of the culture medium liquid is 50-200ml, a main pipe with the inner diameter of 2.5-4cm and the length of 10-25cm, and a gas production collecting pipe with the inner diameter of 2-3.5cm and the length of 3-10cm are selected.
3. The culture device suitable for the detection of the flora on site in the emergencies of public health as recited in claim 1, wherein the display screen comprises a TFT liquid crystal screen and control keys.
4. The culture device suitable for the field flora detection in the sudden public health incident of claim 1, wherein the cover body is a stainless steel cover with an insulating layer adhered to the inner side.
5. The culture device suitable for the field flora detection in sudden public health events according to claim 1, wherein the shell is made of stainless steel material with an insulating layer.
6. The culture device suitable for in-situ flora detection in emergent public health events according to claim 1, wherein the temperature sensor is a contact temperature sensor Pt100 platinum resistor.
7. The culture device suitable for the field flora detection in emergencies of public health as claimed in claim 1, wherein the semiconductor chilling plate is an XLT2389 type semiconductor chilling plate manufactured by Marlow corporation.
8. The culture device suitable for the field flora detection in sudden public health events as claimed in claim 1, wherein the AD chip control sampling circuit calculates the temperature value by adopting a linear interpolation method, and the four-wire connection method constant current source excitation method is used for temperature measurement.
9. The culture device suitable for the field flora detection in emergent public health events according to claim 1, wherein the micro-control chip is a 32-bit AMR micro-control chip S3C 2410; or the memory is 64MNANDFLASH of K9F1208 UDM.
10. The culture device suitable for the field flora detection in emergent public health events as claimed in claim 3, wherein the memory is an extended memory, and 32-bit 64M is formed by SDRAM with model number HY57V561620 manufactured by MICRON.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112522088A (en) * | 2021-01-07 | 2021-03-19 | 南通大学附属医院 | Multifunctional intelligent virus detection specimen sampling and storing integrated system |
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2019
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112522088A (en) * | 2021-01-07 | 2021-03-19 | 南通大学附属医院 | Multifunctional intelligent virus detection specimen sampling and storing integrated system |
| CN112522088B (en) * | 2021-01-07 | 2021-06-25 | 南通大学附属医院 | Multifunctional intelligent virus detection specimen sampling and storing integrated system |
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Granted publication date: 20191227 Termination date: 20220131 |