CN209803157U - Test paper card applied to rapid detection of multi-element small molecular compound - Google Patents

Abstract

The utility model discloses a be applied to test paper card of many first small molecule compound short-term test sets gradually the gold mark micropore, the sample pad that contain the gold mark antibody, the reaction membrane sets up the district and absorbs water and fills up the setting zone along sample liquid flow direction, the reaction membrane sets up the district and is equipped with the reaction membrane, it is equipped with the pad that absorbs water to absorb water to fill up the setting zone, be equipped with the T1 line that the package has beta-lactam class antibiotic antigen, the T2 line that the package has the tetracycline class antigen, the T3 line that the package has the cephalexin antigen and the C line that the package has sheep anti mouse immunoglobulin along sample liquid flow direction on the reaction membrane in proper order. The utility model discloses can once only synchronous detection multicomponent compound, have higher social using value and economic benefits, can play important role in food safety inspection, be applicable to on-the-spot short-term test, be the powerful auxiliary means of national standard method.

Description

test paper card applied to rapid detection of multi-element small molecular compound

Technical Field

The utility model relates to a compound detects technical field, in particular to be applied to test paper card of many first micromolecular compounds short-term test.

Background

Antibiotics mainly refer to a class of chemical substances which are generated by microorganisms and can inhibit or kill other microorganisms, beta-lactam antibiotics, tetracyclines, cefalexin and other antibiotic medicaments are commonly used in the dairy industry to act on the treatment of diseases such as mastitis, metritis and the like of livestock, and antibiotics are also added into feed by producers to improve the feed conversion rate of the livestock. The long-term intake of food containing antibiotics can cause drug accumulation, and when the food reaches a certain concentration, a series of negative effects can be brought to the health of human bodies, such as anaphylactic reaction, damage to gastrointestinal flora balance, enhancement of bacterial drug resistance and the like, so that the health of consumers is harmed, and the export trade of milk and dairy products is also influenced. The beta-lactam antibiotics, tetracyclines, cefalexin and other antibiotics in raw milk of China are seriously remained, and the development of monitoring and detection on the beta-lactam antibiotics, the tetracyclines, the cefalexin and other antibiotics is a very important concern in the development of dairy industry of China.

At present, the detection methods of the antibiotic residues such as beta-lactam antibiotics, tetracyclines, cefalexin and the like in milk and fresh milk can be roughly divided into three categories according to different detection principles. Microbial resistance detection, physicochemical detection methods, and immunological analysis methods. The microbial obstruction detection method mainly comprises a bacteriostatic zone test, a turbidity test and a color-changing detection gold-labeled test strip method, and the microbial detection method has low detection cost, low sensitivity, long time consumption and easy error detection caused by the existence of drug-resistant bacteria: a physicochemical detection method is a main method for detecting antibiotics such as beta-lactam antibiotics, tetracycline, cefalexin and the like at present, and comprises a liquid chromatography, a combined technology of a chromopan and a mass spectrum, a gas chromatography and the like, the physicochemical detection method has high accuracy, but has the defects of high detection cost, complex detection equipment, higher requirement on detection personnel, long detection time and the like, and the currently commonly used immunoassay method mainly has higher detection cost for an enzyme-linked immunosorbent assay and a radioimmunoassay and does not meet the detection requirement of low cost of enterprises.

At present, the field of immunoassay lacks of technologies capable of simultaneously detecting three antibiotics, namely beta-lactam antibiotics, tetracyclines and cefalexin and application thereof. Therefore, in practice, it is necessary to establish a method for detecting a large number of drugs with high sensitivity, simple operation, low cost and wide variety of drugs.

SUMMERY OF THE UTILITY MODEL

The utility model provides a be applied to test paper card of many first micromolecular compounds short-term test can detect these three types of antibiotics of beta-lactam antibiotic, tetracycline, cephalexin simultaneously.

In order to achieve the technical purpose, the technical proposal of the utility model is that:

The utility model provides a be applied to test paper card of many first small molecule compound short-term test sets gradually the gold mark micropore, the sample pad that contain the gold mark antibody along sample liquid flow direction, reaction membrane setting area and absorb water and fill up the setting area, reaction membrane setting area is equipped with the reaction membrane, it is equipped with the pad that absorbs water to fill up the setting area, it has beta-lactam class antibiotic antigen's T1 line, the T2 line that has the tetracycline class antigen to wrap along sample liquid flow direction in proper order to be equipped with on the reaction membrane, the T3 line that has the cephalexin antigen to wrap and the C line that has sheep anti mouse immunoglobulin to wrap; the sample pad with the handing-over department of reaction membrane is equipped with 2 mm's overlap area, the pad that absorbs water with the handing-over department of reaction membrane is equipped with 2 mm's overlap area.

Wherein, preferably, the T1 line, the T2 line, the T3 line and the C line are parallel to each other and perpendicular to the flow direction of the sample liquid.

Wherein, preferably, the gold-labeled micropore depth can be 10-15mm, the sample pad length is 10-15mm, the reaction membrane length is 20-30mm, and the absorbent pad length is 40-45 mm.

Wherein, preferably, the reaction membrane, the sample pad and the absorbent pad are fixed on the bottom plate through double-sided adhesive tapes.

Wherein, preferably, the bottom plate is made of a non-water-absorbing tough material.

Wherein, preferably, the T1 line is 3-6mm from the sample pad, the T2 line is 7-9mm from the sample pad, the T3 line is 12-14mm from the sample pad, and the C line is 17-19mm from the sample pad.

Wherein, preferably, the reaction membrane is a nitrocellulose membrane, and the sample pad is a sample pad made of glass fiber cotton.

The utility model has the advantages that:

(1) And synchronously detecting the multi-component compound at one time.

(2) simple and quick operation. No other reagent is needed in the detection process, and the result can be judged within 3-5 minutes.

(3) The pretreatment of the sample is quick and simple, and the samples can be simultaneously treated in batch.

(4) The result judgment is simple and clear. The macroscopic red detection blot was used as the criterion. One C line blot indicates that the test sample contains beta-lactam antibiotics, tetracyclines and cephalexin, and C, T lines (T1, T2 and T3) four blots indicate that the test sample does not contain beta-lactam antibiotics, tetracyclines and cephalexin.

(5) The detection cost is low.

(6) The method has high application value, can be operated by people, has higher social application value and economic benefit, can play an important role in food safety detection, is suitable for on-site rapid detection, and is a powerful auxiliary means of the national standard method.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

Fig. 1 is a schematic structural diagram of a test paper card for rapid detection of small molecule compounds in the present invention.

In the figure, 1 is a gold-labeled micropore, 2 is a bottom plate, 3 is a sample pad, 4 is a reaction membrane, 5 is a water absorption pad, 6 is a T1 line, 7 is a T2 line, 8 is a T3 line, and 9 is a C line

Detailed Description

The technical solution of the present invention will be described clearly and completely below with reference to specific embodiments of the present invention, and it should be understood that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

As shown in fig. 1, this embodiment provides a be applied to test paper card of many first micromolecule compound short-term test, gold mark micropore 1 that contains the gold mark antibody has set gradually along sample liquid flow direction, sample pad 3, reaction film setting area and absorption pad setting area, reaction film setting area is equipped with reaction film 4, absorption pad setting area is equipped with absorption pad 5, reaction film 4 is last to be equipped with the T1 line 6 that is wrapped with beta-lactam class antibiotic antigen along the sample liquid flow direction in proper order, T2 line 7 that is wrapped with tetracycline class antigen, T3 line 8 that is wrapped with cephalexin antigen and the C line 9 that is wrapped with goat anti mouse immunoglobulin. The beta-lactam antibiotic specific antibody is a beta-lactam antibiotic monoclonal antibody; the tetracycline specific antibody is a tetracycline monoclonal antibody; the cephalexin specific antibody is a cephalexin monoclonal antibody.

Wherein the T1 line 6, the T2 line 7, the T3 line 8 and the C line 9 are parallel to each other and perpendicular to the flow direction of the sample liquid.

Wherein, the depth of the gold-labeled micropore 1 can be 10-15mm, the length of the sample pad 3 is 10-15mm, the length of the reaction membrane 4 is 20-30mm, and the length of the water absorption pad 5 is 40-45 mm.

Wherein, the joint of the sample pad 3 and the reaction membrane 4 is provided with an overlapping area of 2mm, and the joint of the water absorption pad 5 and the reaction membrane 4 is provided with an overlapping area of 2 mm.

Wherein, the reaction film 4, the sample pad 3 and the water absorption pad 5 are fixed on the bottom plate 2 through double-sided adhesive tape.

Wherein, the bottom plate 2 is made of a non-absorbent tough material such as rigid plastic and non-absorbent hard paper, and can be a PVC plate.

Where T1 line 6 was 6mm from the sample pad, T2 line 7 was 8mm from the sample pad, T3 line 8 was 13mm from the sample pad, and C line 9 was 18mm from the sample pad.

Wherein the reaction membrane 4 is a nitrocellulose membrane, and the sample pad 3 is a sample pad 3 made of glass fiber cotton.

The utility model discloses a detection principle: when the sample solution contains melamine, chloramphenicol and aflatoxin M1, in the diffusion process, the melamine, chloramphenicol and aflatoxin M1 in the sample respectively inhibit the three specific antibodies marked by the colloidal gold from being respectively combined with the three antigens on the T line through competitive combination, the T line cannot develop color, the goat anti-mouse immunoglobulin on the C line 9 can be combined with the mixture of the three specific antibodies marked by the colloidal gold, and the C line 9 shows red print. When the sample does not contain the three substances (melamine, chloramphenicol and aflatoxin M1) or the content of the three substances is lower than the detection limit of the kit, the three specific antibodies marked by the colloidal gold can be respectively combined with the three antigens on the T line, the T line shows red blots, when the liquid permeates into the C line 9, the goat anti-mouse immunoglobulin on the C line 9 can be combined with the mixture of the three specific antibodies marked by the colloidal gold, and the C line 9 shows red blots.

The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (7)

1. A test paper card applied to rapid detection of multi-element small molecule compounds is characterized in that: the kit comprises a sample liquid, and is characterized in that a gold-labeled micropore containing a gold-labeled antibody, a sample pad, a reaction membrane setting area and a water absorption pad setting area are sequentially arranged along the flow direction of the sample liquid, the reaction membrane setting area is provided with a reaction membrane, the water absorption pad setting area is provided with a water absorption pad, and the reaction membrane is sequentially provided with a T1 line coated with a beta-lactam antibiotic antigen, a T2 line coated with a tetracycline antigen, a T3 line coated with a cephalexin antigen and a C line coated with goat anti-mouse immunoglobulin along the flow direction of the sample liquid; the sample pad with the handing-over department of reaction membrane is equipped with 2 mm's overlap area, the pad that absorbs water with the handing-over department of reaction membrane is equipped with 2 mm's overlap area.

2. The test paper card applied to the rapid detection of the multielement small molecule compound according to claim 1, characterized in that: the T1 line, the T2 line, the T3 line, and the C line are parallel to each other and perpendicular to the flow direction of the sample liquid.

3. The test paper card applied to the rapid detection of the multielement small molecule compound according to claim 1, characterized in that: the gold-labeled micro-pores may have a depth of 10-15mm, the sample pad may have a length of 10-15mm, the reaction membrane may have a length of 20-30mm, and the absorbent pad may have a length of 40-45 mm.

4. The test paper card applied to the rapid detection of the multielement small molecule compound according to claim 1, characterized in that: the reaction membrane, the sample pad and the water absorption pad are fixed on the bottom plate through double-sided adhesive tapes.

5. The test paper card applied to the rapid detection of the multielement small molecule compound according to claim 4, characterized in that: the bottom plate is made of a non-water-absorbing tough material.

6. The test paper card applied to the rapid detection of the multielement small molecule compound according to claim 1, characterized in that: the T1 line is 3-6mm from the sample pad, the T2 line is 7-9mm from the sample pad, the T3 line is 12-14mm from the sample pad, and the C line is 17-19mm from the sample pad.

7. The test paper card applied to the rapid detection of the multielement small molecule compound according to claim 1, characterized in that: the reaction membrane is a nitrocellulose membrane, and the sample pad is made of glass fiber cotton.