CN209803154U

CN209803154U - Synchronous rapid detection card for detecting multiple mycotoxins in feed

Info

Publication number: CN209803154U
Application number: CN201920241958.8U
Authority: CN
Inventors: 吴小平
Original assignee: Beijing Cheerkang Biotechnology Co Ltd
Current assignee: Beijing Cheerkang Biotechnology Co Ltd
Priority date: 2019-02-26
Filing date: 2019-02-26
Publication date: 2019-12-17
Anticipated expiration: 2029-02-26

Abstract

The utility model discloses a synchronous short-term test fodder in many mycotoxin detect card has set gradually along sample liquid flow direction and has added appearance district, reaction film setting district and the pad setting district that absorbs water, it is equipped with the sample pad in the appearance district to add, be equipped with the reaction film in the reaction film setting district, it is equipped with the pad that absorbs water in the pad setting district to absorb water, the sample pad with be equipped with the combination pad in proper order between the reaction film and fill up with higher speed, be equipped with the sample hole on the sample pad, be equipped with the T line that the parcel has mycotoxin antigen and the C line that the parcel has the carrier protein antibody along sample liquid flow direction on the reaction film in proper order, T line and the total three groups of C line are used for detecting aflatoxin B1, vomitoxin, zearalenone respectively. The utility model has the advantages that the sample pretreatment is rapid and simple, and the samples can be simultaneously treated in batches; and can synchronously detect the multi-component compound at one time.

Description

Synchronous rapid detection card for detecting multiple mycotoxins in feed

Technical Field

The utility model relates to a compound detects technical field, in particular to synchronous short-term test fodder in many mycotoxin detect card.

Background

Aflatoxin B1(AFB1), vomitoxin (DON) and Zearalenone (ZEA) mainly pollute cereal crops and food products such as wheat, barley and corn, and people and animals can generate wide toxic effects after eating the cereal crops and food products polluted by the aflatoxin, so that acute toxic symptoms such as anorexia, vomiting, diarrhea, fever, instable standing and slow reaction can be caused, and the hematopoietic system is damaged and the death is caused in severe cases.

The chemical detection methods of aflatoxin B1, vomitoxin and zearalenone comprise Thin Layer Chromatography (TLC), Gas Chromatography (GC), gas spectrum-mass spectrum online (GC/MS), High Pressure Liquid Chromatography (HPLC), high pressure liquid chromatography-mass spectrum online (HPLC/MS) and the like. The pretreatment of the methods utilizes liquid-liquid distribution, and the conventional SPE column purification and separation have the defects of complicated treatment process, poor purification effect, much waste of organic solvent, long required time, requirement of professional operation and the like in different degrees, thereby seriously hindering the popularization of the detection method and further failing to realize field detection. The mycotoxin (aflatoxin B1, vomitoxin and zearalenone) residue detection card provided by the invention is simple to operate, can be operated by people, can judge whether the result is positive or negative, and has high application value and great economic and social benefits.

SUMMERY OF THE UTILITY MODEL

The utility model provides a synchronous short-term test fodder in many mycotoxin detect card, the processing procedure is loaded down with trivial details, purifying effect is poor, organic solvent is extravagant many, required time is long and need professional operation scheduling problem when solving current mycotoxin and detecting.

In order to solve the technical problem, the utility model adopts the technical scheme that:

The utility model provides a synchronous short-term test fodder in many mycotoxin detect card has set gradually along sample liquid flow direction and has added appearance district, reaction film setting district and the pad setting district that absorbs water, it is equipped with the sample pad in the appearance district to add, be equipped with the reaction film in the reaction film setting district, it is equipped with the pad that absorbs water in the pad setting district to absorb water, the sample pad with be equipped with the combination pad in proper order between the reaction film and fill up with higher speed, be equipped with the sample hole on the sample pad, be equipped with the T line that the parcel has mycotoxin antigen and the C line that the parcel has the carrier protein antibody along sample liquid flow direction on the reaction film in proper order, T line and the total three groups of C line are used for detecting aflatoxin B1, vomitoxin, zearalenone respectively.

Wherein, preferably, the T line is parallel to the C line and perpendicular to the flow direction of the sample liquid.

Wherein, preferably, the water absorption pad is covered with a sealing cover.

Wherein, preferably, the length of the sample pad is 16mm, the length of the combination pad and the length of the acceleration pad are both 4mm, the length of the reaction membrane is 25mm, and the length of the absorbent pad is 16 mm.

wherein, preferably, the T line is 6-8mm away from the accelerating pad, and the C line is 4-6mm away from the water absorption pad.

Wherein, preferably, the reaction membrane, the sample pad and the absorbent pad are fixed on the bottom plate through double-sided adhesive tapes.

The utility model has the advantages that:

(1) And synchronously detecting the multi-component compound at one time.

(2) The operation is rapid, other reagents are not needed in the detection process, and the result can be judged within 5-8 minutes.

(3) The pretreatment of the sample is quick and simple, and the samples can be simultaneously treated in batch.

(4) The result judgment is simple and clear. The test card uses a red test blot visible to the naked eye as a criterion. One C-line blot indicates that the test sample contains mycotoxin, and three groups of C, T lines and two blots indicate that the test sample does not contain mycotoxin.

(5) the detection cost is low, the application value is high, and the method can be operated by people; the method has higher social application value and economic benefit, can play an important role in food safety detection, is suitable for on-site rapid detection, and is a powerful auxiliary means of the national standard method.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

Fig. 1 is a schematic structural view of the multi-element lean meat detecting card for synchronous and rapid detection of the present invention;

Fig. 2 is a view along direction a of fig. 1.

In the figure, 1, a bottom plate, 2, a reaction membrane, 3, a sample pad, 4, a bonding pad, 5, an acceleration pad, 6, a water absorption pad, 7, a sealing cover, 8, a T line, 9, a C line, and 10, a sample hole

Detailed Description

The technical solution of the present invention will be described clearly and completely below with reference to specific embodiments of the present invention, and it should be understood that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

As shown in fig. 1, this embodiment provides a card for detecting multiple mycotoxins in feed synchronously and rapidly, a sample adding region, a reaction membrane setting region and a water absorption pad setting region are sequentially arranged along a sample liquid flowing direction, a sample pad 3 is arranged in the sample adding region, a reaction membrane 2 is arranged in the reaction membrane setting region, a water absorption pad 6 is arranged in the water absorption pad setting region, a combination pad 4 and an acceleration pad 5 are sequentially arranged between the sample pad 3 and the reaction membrane 2, a sample hole 10 is arranged on the sample pad 3, a T-line 8 coated with a mycotoxin antigen and a C-line 9 coated with a carrier protein antibody are sequentially arranged on the reaction membrane 2 along the sample liquid flowing direction, and the T-line 8 and the C-line 9 have three groups in total and are respectively used for detecting aflatoxin B1, vomitoxin and zearalenone. And the antibody specific for the compound having mycotoxin is a monoclonal antibody. The carrier protein antibody is a goat anti-mouse polyclonal antibody.

Wherein the T line 8 is parallel to the C line 9 and perpendicular to the flow direction of the sample liquid. The T-line 8 is adjacent to the acceleration pad 5 and the C-line 9 may be adjacent to the absorbent pad 6.

Wherein, the absorbent pad 6 is covered with a cover 7. The closing cap 7 is waterproof material for each subassembly of fixed detection card avoids the material in sample reagent and the external environment to react, influences the detection effect of detection card.

Wherein, the length of the sample pad 3 is 16mm, the lengths of the combination pad 4 and the acceleration pad 5 are both 4mm, the length of the reaction membrane 2 is 25mm, and the length of the water absorption pad 6 is 16 mm.

Wherein, the T line 8 is 7mm away from the accelerating pad, and the C line 9 is 5mm away from the water absorption pad.

Wherein, the reaction film 2, the sample pad 3 and the water absorption pad 6 are fixed on the bottom plate 1 through double-sided adhesive tape.

The utility model discloses a detection principle:

when the sample solution contains mycotoxin, in the diffusion process, the mycotoxin in the sample inhibits the specific antibody marked by the colloidal gold from being respectively combined with the mycotoxin antigen on the T line through competitive combination, the T line cannot be developed, the carrier protein antibody of the C line can be respectively combined with the specific antibody marked by the colloidal gold, and the C line shows red blot. When the sample does not contain mycotoxin or the content of the mycotoxin is lower than the detection limit of the detection card, the specific antibody marked by the colloidal gold is respectively combined with the mycotoxin antigen on the T line, the T line shows red blots, when liquid permeates into the C line, the anti-carrier protein antibody on the C line can be combined with the specific antibody marked by the colloidal gold, and the C line shows red.

The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The utility model provides a synchronous short-term test fodder in many mycotoxin detect card which characterized in that: set gradually along sample liquid flow direction and add appearance district, reaction membrane setting area and absorb water and fill up the setting area, be equipped with the sample pad in the appearance district with adding, be equipped with the reaction membrane in the reaction membrane setting area, it is equipped with the pad that absorbs water in the setting area to absorb water, the sample pad with be equipped with the combination pad in proper order between the reaction membrane and fill up with higher speed, the sample is filled up and is equipped with the sample hole, be equipped with the T line that the parcel has the mycotoxin antigen and the C line that the parcel has the carrier protein antibody along sample liquid flow direction on the reaction membrane in proper order, the total three groups of T line and C line are used for detecting aflatoxin B1, vomitoxin, zearalenone respectively.

2. The detection card for synchronously and rapidly detecting the multiple mycotoxins in the feed according to claim 1, is characterized in that: the T line is parallel to the C line and perpendicular to the flow direction of the sample liquid.

3. The detection card for synchronously and rapidly detecting the multiple mycotoxins in the feed according to claim 1, is characterized in that: the water absorption pad is covered with a sealing cover.

4. The detection card for synchronously and rapidly detecting the multiple mycotoxins in the feed according to claim 1, is characterized in that: the length of sample pad is 16mm, the length of combination pad with the acceleration pad is 4mm, the length of reaction film is 25mm, the length of absorbent pad is 16 mm.

5. the detection card for synchronously and rapidly detecting the multiple mycotoxins in the feed according to claim 1, is characterized in that: the distance between the T line and the acceleration pad is 6-8mm, and the distance between the C line and the water absorption pad is 4-6 mm.

6. The detection card for synchronously and rapidly detecting the multiple mycotoxins in the feed according to claim 1, is characterized in that: the reaction membrane, the sample pad and the water absorption pad are fixed on the bottom plate through double-sided adhesive tapes.