CN209803155U - Synchronous rapid detection multielement lean meat essence detection card - Google Patents
Synchronous rapid detection multielement lean meat essence detection card Download PDFInfo
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- CN209803155U CN209803155U CN201920241960.5U CN201920241960U CN209803155U CN 209803155 U CN209803155 U CN 209803155U CN 201920241960 U CN201920241960 U CN 201920241960U CN 209803155 U CN209803155 U CN 209803155U
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Abstract
The utility model discloses a synchronous short-term test many units lean meat essence detects card has set gradually along sample liquid flow direction and has added appearance district, reaction membrane setting district and water absorption pad setting district, be equipped with the sample pad in adding the appearance district, be equipped with the reaction membrane in the reaction membrane setting district, it is equipped with the water absorption pad in the setting district to absorb water pad, the sample pad with it has the combination pad and fills up with higher speed to have designed in proper order between the reaction membrane, the sample stacks up and is equipped with the sample hole, be equipped with the T line that the parcel has lean meat essence antigen and the C line that the parcel has the carrier protein antibody along sample liquid flow direction on the reaction membrane in proper order. The utility model discloses disposable synchronous detection multicomponent compound has higher social using value and economic benefits, can play the great effect in food safety inspection, is applicable to on-the-spot short-term test, is the powerful auxiliary means of national standard method.
Description
Technical Field
The utility model relates to a compound detects technical field, in particular to synchronous short-term test many first lean meat essence detects card.
Background
Clenbuterol is a general term for the medicine application of a class of animals, substances capable of promoting the growth of the clenbuterol and inhibiting the growth of animal fat can be called as 'clenbuterol', and the clenbuterol is a very cheap medicine and has a very good effect on reducing fat and increasing the clenbuterol (Lean Mass). In China, the general term clenbuterol refers to clenbuterol, ractopamine and salbutamol.
Clenbuterol (Clenbuterol), ractopamine (ractopamine) and Salbutamol (Salbutamol) belong to phenethyl alcohol amine beta-agonists, are mainly used for dilating bronchus and increasing lung flux in clinic, and can treat symptoms such as bronchial asthma, obstructive pulmonary disease, smooth muscle spasm and shock; the medicine can also be used as growth promoter for cattle, sheep, pigs, poultry and the like, and once in all, the medicine is widely applied to the production of beasts and birds. In recent years, clenbuterol and salbutamol as feed additives are increasingly used illegally in animal husbandry. Although clenbuterol can improve the unit economic value of pigs, clenbuterol, ractopamine, salbutamol and preparations thereof are definitely forbidden in China to be used in the feeding process of food animals because clenbuterol is easy to accumulate and remain in animal livers and can enter human bodies through food chains, and the health of the human bodies is seriously damaged. It is therefore necessary to enhance the detection of stimulant residues in animal food.
One of the existing methods for detecting clenbuterol, ractopamine and salbutamol is a chromatographic analysis method, mainly including Thin Layer Chromatography (TLC), Gas Chromatography (GC), High Performance Liquid Chromatography (HPLC), gas-mass chromatography (GC/MS), liquid-mass chromatography (HPLC/MS) and the like, and is not suitable for on-site monitoring and sample screening due to complex instrument and equipment and complicated process. The other is immunological method, such as enzyme linked immunosorbent assay (ELISA), which has the disadvantages of long detection time, high cost and difficult popularization and application in basic units. Moreover, both methods require the operation of a skilled person.
SUMMERY OF THE UTILITY MODEL
The utility model provides a synchronous short-term test many units lean meat essence detects card solves the unsuitable on-the-spot monitoring of current lean meat essence detection and sample examination and the longer, the higher problem of expense of check-out time.
In order to solve the technical problem, the utility model adopts the technical scheme that:
The utility model provides a synchronous short-term test many units lean meat essence detects card has set gradually along sample liquid flow direction and has added appearance district, reaction membrane setting district and water absorption pad setting district, be equipped with the sample pad in adding the appearance district, be equipped with the reaction membrane in the reaction membrane setting district, it is equipped with the water absorption pad in the setting district to absorb water pad, the sample pad with it has the combination pad and fills up with higher speed to have designed in proper order between the reaction membrane, the sample stacks up and is equipped with the sample hole, be equipped with the T line that the parcel has lean meat essence antigen and the C line that the parcel has the carrier protein antibody along sample liquid flow direction on the reaction membrane in proper order.
Wherein, preferably, the T line is parallel to the C line and perpendicular to the flow direction of the sample liquid.
Preferably, three parallel C lines and T lines for detecting clenbuterol, ractopamine and salbutamol are arranged on the reaction membrane.
Wherein, preferably, the water absorption pad is covered with a sealing cover.
wherein, preferably, the length of the sample pad is 16mm, the length of the combination pad and the length of the acceleration pad are both 4mm, the length of the reaction membrane is 25mm, and the length of the absorbent pad is 16 mm.
Wherein, preferably, the sample pad and the conjugate pad are provided with an overlapping area of 2mm, the conjugate pad and the acceleration pad are provided with an overlapping area of 2mm, and the acceleration pad and the reaction membrane are provided with an overlapping area of 2 mm.
Wherein, preferably, the T line is 6-8mm away from the accelerating pad, and the C line is 4-6mm away from the water absorption pad.
wherein, preferably, the reaction membrane, the sample pad and the absorbent pad are fixed on the bottom plate through double-sided adhesive tapes.
Wherein, preferably, an isolating membrane is arranged between the reaction membrane and the bottom plate.
The utility model has the advantages that:
(1) And synchronously detecting the multi-component compound at one time.
(2) Simple and quick operation. In the detection process, other reagents are not needed, the detection card is leveled to the water discharge position, and the result can be judged within 3-5 minutes.
(3) The pretreatment of the sample is quick and simple, and the samples can be simultaneously treated in batch.
(4) The judgment result is simple, and the detection cost is low.
(5) The detection card is operable by all people, has higher social application value and economic benefit, can play an important role in food safety detection, is suitable for on-site rapid detection, and is a powerful auxiliary means of the national standard method.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
Fig. 1 is a schematic structural view of the multi-element lean meat detecting card for synchronous and rapid detection of the present invention;
Fig. 2 is a view along direction a of fig. 1.
In the figure, 1, a bottom plate, 2, a reaction membrane, 3, a sample pad, 4, a bonding pad, 5, an acceleration pad, 6, a water absorption pad, 7, a sealing cover, 8, a T line, 9, a C line, and 10, a sample hole
Detailed Description
The technical solution of the present invention will be described clearly and completely below with reference to specific embodiments of the present invention, and it should be understood that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
As shown in fig. 1, this embodiment provides the utility model provides a synchronous short-term test many units lean meat essence detects card, sample solution flow direction has set gradually with the appearance district, reaction membrane 2 sets up district and water absorption pad 6 sets up the district, be equipped with sample pad 3 in the sample district, be equipped with reaction membrane 2 in the reaction membrane 2 sets up the district, it is equipped with water absorption pad 6 in the district to absorb water pad 6 sets up, it has conjugate pad 4 and acceleration pad 5 to design in proper order between sample pad 3 and the reaction membrane 2, be equipped with sample hole 10 on the sample pad 3, be equipped with T line 8 that the parcel was had lean meat essence antigen and the C line 9 that the parcel had carrier body protein antibody along sample solution flow direction on the reaction membrane 2 in proper order. The specific antibody of the compound with the clenbuterol is a monoclonal antibody, and the carrier protein antibody is a Bovine Serum Albumin (BSA) polyclonal antibody.
Wherein the T line 8 is parallel to the C line 9 and perpendicular to the flow direction of the sample liquid. The T-line 8 is adjacent to the acceleration pad 5 and the C-line 9 may be adjacent to the absorbent pad 6.
Wherein, three groups of parallel C lines 9 and T lines 8 for detecting clenbuterol, ractopamine and salbutamol are arranged on the reaction film 2.
Wherein, the absorbent pad 6 is covered with a cover 7. The closing cap 7 is waterproof material for each subassembly of fixed detection card avoids the material in sample reagent and the external environment to react, influences the detection effect of detection card.
Wherein, the length of the sample pad 3 is 16mm, the lengths of the combination pad 4 and the acceleration pad 5 are both 4mm, the length of the reaction membrane 2 is 25mm, and the length of the water absorption pad 6 is 16 mm.
In order to ensure the continuity of the flow of the sample liquid, the sample pad 3 and the conjugate pad 4 are provided with an overlapping area of 2mm, the conjugate pad 4 and the acceleration pad 5 are provided with an overlapping area of 2mm, and the acceleration pad 5 and the reaction membrane 2 are provided with an overlapping area of 2 mm.
Wherein, the distance between the T line 8 and the acceleration pad is 56-8mm, and the distance between the C line 9 and the water absorption pad is 64-6 mm.
Wherein, the reaction film 2, the sample pad 3 and the water absorption pad 6 are fixed on the bottom plate 1 through double-sided adhesive tape.
Wherein, an isolating film is arranged between the reaction film 2 and the bottom plate 1. The isolating membrane is made of high molecular materials and is used for preventing organic solvents in the adhesive from damaging the coating protein and the coating antibody.
The utility model discloses a detection principle: when the sample solution contains clenbuterol (clenbuterol, ractopamine and salbutamol), in the diffusion process, the clenbuterol (clenbuterol, ractopamine and salbutamol) in the sample inhibits the specific antibody marked by the colloidal gold from being combined with the antigen with the clenbuterol (clenbuterol, ractopamine and salbutamol) on the T line 8 respectively through competitive combination, the T line 8 cannot develop color, the carrier protein antibody of the C line 9 can be combined with the specific antibody marked by the colloidal gold, and the C line 9 shows red print. When the sample does not contain clenbuterol (clenbuterol, ractopamine and salbutamol) or the content of the clenbuterol is lower than the detection limit of the detection card, the specific antibody marked by the colloidal gold is respectively combined with the antigen with the clenbuterol (clenbuterol, ractopamine and salbutamol) on the T line 8, the T line 8 shows a red print, when the liquid permeates into the C line 9, the anti-carrier protein antibody on the C line 9 can be combined with the specific antibody marked by the colloidal gold, and the C line 9 shows a red color.
the above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (9)
1. The utility model provides a synchronous short-term test many units lean meat essence detects card which characterized in that: have set gradually along sample liquid flow direction and have added appearance district, reaction membrane setting district and water absorption pad setting area, be equipped with the sample pad in the appearance district with adding, be equipped with the reaction membrane in the reaction membrane setting district, it is equipped with the water absorption pad in the setting district to absorb water, the sample pad with it has the combination pad and fills up with higher speed to have designed in proper order between the reaction membrane, the sample stacks up and is equipped with the sample hole, be equipped with the T line that the parcel has clenbuterol antigen and the C line that the parcel has the carrier protein antibody along sample liquid flow direction on the reaction membrane in proper order.
2. The card of claim 1, wherein the card comprises: the T line is parallel to the C line and perpendicular to the flow direction of the sample liquid.
3. The card of claim 2, wherein the card comprises: and three groups of parallel C lines and T lines for detecting clenbuterol, ractopamine and salbutamol are arranged on the reaction membrane.
4. The card of claim 1, wherein the card comprises: the water absorption pad is covered with a sealing cover.
5. The card of claim 1, wherein the card comprises: the length of sample pad is 16mm, the length of combination pad with the acceleration pad is 4mm, the length of reaction film is 25mm, the length of absorbent pad is 16 mm.
6. The card of claim 5, wherein the card comprises: the sample pad with the combination pad is equipped with 2 mm's overlap area, the combination pad with the pad is equipped with 2 mm's overlap area with accelerating, the pad with the reaction membrane is equipped with 2 mm's overlap area with accelerating.
7. The card of claim 1, wherein the card comprises: the distance between the T line and the acceleration pad is 6-8mm, and the distance between the C line and the water absorption pad is 4-6 mm.
8. The card of claim 1, wherein the card comprises: the reaction membrane, the sample pad and the water absorption pad are fixed on the bottom plate through double-sided adhesive tapes.
9. The card of claim 8, wherein the card comprises: and an isolating membrane is arranged between the reaction membrane and the bottom plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920241960.5U CN209803155U (en) | 2019-02-26 | 2019-02-26 | Synchronous rapid detection multielement lean meat essence detection card |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920241960.5U CN209803155U (en) | 2019-02-26 | 2019-02-26 | Synchronous rapid detection multielement lean meat essence detection card |
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| Publication Number | Publication Date |
|---|---|
| CN209803155U true CN209803155U (en) | 2019-12-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920241960.5U Expired - Fee Related CN209803155U (en) | 2019-02-26 | 2019-02-26 | Synchronous rapid detection multielement lean meat essence detection card |
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| CN (1) | CN209803155U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111929442A (en) * | 2020-08-13 | 2020-11-13 | 北京测尔康生物技术有限公司 | Novel coronavirus antigen and antibody detection kit |
| CN111983223A (en) * | 2020-08-19 | 2020-11-24 | 北京测尔康生物技术有限公司 | Kit for detecting drug residues of sedaxane in agricultural products |
-
2019
- 2019-02-26 CN CN201920241960.5U patent/CN209803155U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111929442A (en) * | 2020-08-13 | 2020-11-13 | 北京测尔康生物技术有限公司 | Novel coronavirus antigen and antibody detection kit |
| CN111983223A (en) * | 2020-08-19 | 2020-11-24 | 北京测尔康生物技术有限公司 | Kit for detecting drug residues of sedaxane in agricultural products |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20191217 |
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| CF01 | Termination of patent right due to non-payment of annual fee |