CN204241478U - For detecting the immuno-chromatographic test paper strip whether being mixed with certain animal flesh in meat - Google Patents
For detecting the immuno-chromatographic test paper strip whether being mixed with certain animal flesh in meat Download PDFInfo
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- CN204241478U CN204241478U CN201420784407.3U CN201420784407U CN204241478U CN 204241478 U CN204241478 U CN 204241478U CN 201420784407 U CN201420784407 U CN 201420784407U CN 204241478 U CN204241478 U CN 204241478U
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Abstract
The utility model provides that a kind of it comprises hard floor for detecting the immuno-chromatographic test paper strip whether being mixed with certain animal flesh in meat, and its upper surface is folded is provided with glue-line, glue-line is pasted successively sample pad, pad, porous nitrocellulose film and absorbent filter; Sample pad is glass fibre membrane or polyester film; Pad is the fritted glass tunica fibrosa of absorption labelled antibody, and labelled antibody is the antibody of the specificity albumen with label; Cellulose nitrate membrane aperture 2-10 μm, on it, vertical liquid chromatographic flow direction is provided with banded surveyed area and control zone, and surveyed area internal fixtion has the antibody of described specificity albumen, and control zone internal fixtion has two of labelled antibody to resist; Sample pad downstream end is overlapped on the upstream extremity of pad, and pad downstream end is overlapped on the upstream extremity of nitrocellulose filter, and the upstream extremity of absorbent filter is overlapped on the downstream end of nitrocellulose filter.Whether the utility model can be used in detection meat adulterated.
Description
Technical field
The utility model relates to a kind of for detecting in meat whether be mixed with certain animal flesh (namely, whether adulteratedly detect in meat) immuno-chromatographic test paper strip, the meat such as other animal (including but not limited to pig, duck, chicken, horse) of mixing in the meat such as animal (including but not limited to ox, sheep) can be detected, belong to the method for quick of field of food safety.
Background technology
Due to interests temptation, order about some lawless persons ox, sheep, etc. the raw material of adulterate in high price meat products meat or the non-edible animal at a low price such as pig, duck, cat, fox, otter, with essence process, these pretend to be high price meat to sell after meat at a low price.These adulterated behaviors not only can cause damage to the economic interests of consumer, even may cause actual bodily harm for some to the consumer of certain animal allergy, and What is more can relate to ethnic minority issue and cause severe social influence.Along with the intervention of national interested regulatory authorities and the raising of consumer's consciousness, create strong demand to whether Meat ingredients in consumption of meat is adulterated.
People to have carried out for many years to the research of meat adulteration, current technology is mainly analyzed based on to specific proteins, DNA, fatty acid equimolecular structure, sequence or composition in meat, and the technological means related to comprises enzyme linked immunological, PCR, electrophoresis, chromatogram, bio-sensing analysis etc.Wherein, DNA detection method and euzymelinked immunosorbent assay (ELISA) are at present for the maturation method of meat detection.DNA molecular has the advantage such as high specificity, higher thermal stability between conservative type highly, kind, and PCR, can independently for analyzing the composition whether containing certain class animal in sample by detecting special DNA fragmentation; But DNA technique needs expensive equipment, the operating personnel of specialty, testing process is loaded down with trivial details and time-consuming many, and the high sensitivity of DNA technique causes it easily contaminated, this has higher requirement to the collection of sample on the one hand, also have higher requirement to the environment detected on the other hand, these shortcomings cause DNA meat adulteration detection technique can only apply on a small quantity in developed regions, and are not suitable for large-scale promotion and application.Euzymelinked immunosorbent assay (ELISA) belongs to immunoassay technology, and in meat adulteration, the main specific proteins detecting animal in meat, has higher susceptibility.Euzymelinked immunosorbent assay (ELISA) belongs to very ripe detection method, but it has following shortcoming: need configuration detection equipment, need professional to operate, detecting step is complicated and time-consuming, be applicable to the laboratory of certain technology and financial strength.Other are the method such as electrophoresis, chromatogram such as, due to a variety of causes, is not developed.
Immunochromatography technique belongs to a kind of scene immunologic detection method fast, and modal immunochromatography technique is colloidal gold immunochromatographimethod, which utilizes the specificity of antigen-antibody reaction and the naked eyes observability of collaurum.Immune colloidal gold technique (Immune colloidal gold technique) is a kind of novel immunolabelling technique being applied to antigen-antibody using collaurum as tracer label thing, and english abbreviation is: GICA.Collaurum is by gold chloride (HAuCl
4) at reductive agent as under the effect such as white phosphorus, ascorbic acid, sodium citrate, tannic acid, polymerization becomes the gold grain of specific size, and becomes a kind of stable colloidal state due to electrostatic interaction, is called collaurum.Collaurum is electronegative under mild alkaline conditions, can be formed firmly be combined, because this combination is electrostatical binding, so do not affect the biological nature of protein with the positive charge group of protein molecule.Collaurum except with protein bound except, can also be combined, as SPA, PHA, ConA etc. with other biomacromolecules many.According to some physical behaviors of collaurum, as high electron density, grain size, shape and color reaction, add immunity and the biological characteristics of bond, thus make collaurum be widely used in the detection in the fields such as clinical diagnosis, family's self-inspection, food security, farming animals animal husbandry.Colloidal gold immunochromatographimethod technology tool has the following advantages: fast easy to use, and be convenient to basic unit and use and onsite application, institute responds and can complete in 15 minutes; Cost is low, does not need special instrument and equipment; Applied range, can adapt to multiple testing conditions; Can carry out multinomial detection, if the more difficult acquisition of positive sample, multinomial detection can save sample, reduces costs; Label is stablized, and mark sample stores more than 2 year year at 4 DEG C, no signal relaxation phenomenon; Collaurum, originally as redness, does not need to add chromogenic agents, eliminates the step of enzyme target carcinogenicity substrate and stop buffer, to human non-toxic's evil.
The structure of traditional colloidal gold immuno-chromatography test paper strip, normally on end liner, sequentially arrange sample pad, the pad being provided with colloid gold particle label, nitrocellulose filter and adsorptive pads, nitrocellulose filter is provided with detection line and control line.For the colloidal gold immuno-chromatography test paper strip of concrete test item, the basis of traditional test strips structure can be changed to some extent to agents useful for same material and concrete structure.Such as:
CN 202814977 U discloses a kind of bladder chalone C colloidal gold immunochromatographydetection detection test paper bar, which employs biotin-avidin micro-signal amplification system, in detection sample in bladder chalone C process, expand the signal of bladder chalone C, increase detection sensitivity, avoid occurring false the moon or undetected because signal is too weak.
CN 202548130 U discloses a kind of immuno-chromatographic test paper strip of improvement, a RFID radio-frequency card has wherein been installed on adsorptive pads, the data that this RFID radio-frequency card produces are read instrument by RFID UPT turned in illumination instrument and are read, and detection precision is improved.
CN 202735347 U discloses a kind of novel clinical and detects immuno-chromatographic test paper strip, this test strips comprises base plate, base plate is overlapped with strip sample pad, circular sample pad, pad, nitrocellulose filter and thieving paper successively mutually, red blood cell filtration film is provided with below strip sample pad and circular sample pad, nitrocellulose filter is coated with detection line and nature controlling line, the prior art is by changing sample pad shape, realize red blood cell to be separated with the effective of serum when whole blood sample detects, improve the accuracy of testing result.
CN 203396776 U discloses a kind of HIT antibody test immunofluorescence chromatograph test strip, this test strips is made up of the sample pad mutually overlapped on liner plate successively, reaction film and absorption pad, sample pad two ends are respectively arranged with first and add sampling point and second and add sampling point, reaction film is fixed with an inner quality control band and at least one antibody test band, this HIT antibody test immunofluorescence chromatograph test strip, sxemiquantitative can be realized or quantitatively detect, for the diagnosis of heparin-induced thrombocytopenia and related complication provides comparatively reliable and practical method.
CN 202676702 U discloses a kind of colloidal gold immunochromatographimethod quantitative testing test paper, comprise reagent draw-in groove, liner plate, sample pad, colloidal gold pad, nitrocellulose filter, thieving paper, reagent draw-in groove comprises upper shell and lower house, upper shell is connected with described lower house buckle, upper shell is provided with the first hole and the second hole, sample pad is placed in below the first hole, nitrocellulose filter is placed in below the second hole, liner plate is fixed in reagent draw-in groove, sample pad, colloidal gold pad, nitrocellulose filter and thieving paper are arranged in order and are connected to liner plate upper surface, nitrocellulose filter is provided with at least three detection lines, antigen or the antibody of known quantity is coated with in detection line, by quantitative combination and the detection of many detection lines and detected sample, realize the quantitative detection to detected sample.
But the test strips of these prior aries above-mentioned is all for specifically designing, and whether these test strips can not be used for detecting meat adulterated, cannot adapt to the quick detection demand in meat product safety field.Have no the report about meat adulteration Fast Detection Technique at present.
Utility model content
Whether adulterated fundamental purpose of the present utility model be to provide a kind of for detecting in meat immuno-chromatographic test paper strip, apply this test strips and can detect in certain animal (including but not limited to ox, sheep etc.) meat whether be mixed with other animal (including but not limited to pig, duck, chicken, horse etc.) meat, to meet the field quick detection demand of every aspect fast, easily.
For achieving the above object, the utility model uses the sero-abluminous specific antibody of different animals to mark, and other specific antibody carries out NC film bag quilt, makes the immuno-chromatographic test paper strip for detection of adulterations in meat.
Specifically, the utility model provides a kind of for detecting the immuno-chromatographic test paper strip whether being mixed with certain animal flesh in meat, this immuno-chromatographic test paper strip comprises hard floor, one deck glue-line is provided with in the upper surface of hard floor is folded, according to liquid chromatography(LC) flowing upstream and downstream direction, glue-line is pasted with successively sample pad, pad, porous nitrocellulose film and absorbent filter; Wherein:
Described sample pad is glass fibre membrane or polyester film;
Described pad is the fritted glass tunica fibrosa of absorption labelled antibody, and described labelled antibody is the antibody of the specificity albumen with certain animal described in label;
Described porous nitrocellulose film is the porous nitrocellulose film of aperture 2-10 μm, on porous nitrocellulose film, vertical liquid chromatographic flow direction is provided with banded surveyed area and control zone, surveyed area internal fixtion has the antibody of described specificity albumen, control zone internal fixtion has two of labelled antibody to resist, such as sheep anti-mouse igg;
Sample pad downstream end is overlapped on the upstream extremity of pad, and pad downstream end is overlapped on the upstream extremity of porous nitrocellulose film, and the upstream extremity of absorbent filter is overlapped on the downstream end of porous nitrocellulose film.
According to specific embodiments of the present utility model, of the present utility modelly also can comprise getting stuck for detecting the immuno-chromatographic test paper strip whether being mixed with certain animal flesh in meat, get stuck interior accommodating hard floor, sample pad, pad, porous nitrocellulose film and absorbent filter, and application of sample window is offered in the position of the upper surface counter sample pad that gets stuck, watch window is offered in the corresponding surveyed area of porous nitrocellulose film and the position of control zone.
Whether, according to specific embodiments of the present utility model, to be of the present utility modelly mixed with in the immuno-chromatographic test paper strip of certain animal flesh for detecting in meat, described sample pad is fritted glass tunica fibrosa or the porous polyester film of aperture 2-8 μm; Described pad is the fritted glass tunica fibrosa of aperture 2-8 μm.
Whether, according to specific embodiments of the present utility model, to be of the present utility modelly mixed with in the immuno-chromatographic test paper strip of certain animal flesh for detecting in meat, certain animal described is pig, duck, chicken, fox, otter or horse.
Whether, according to specific embodiments of the present utility model, to be of the present utility modelly mixed with in the immuno-chromatographic test paper strip of certain animal flesh for detecting in meat, described specificity albumen is immunoglobulin (Ig) or seralbumin or troponin.
According to specific embodiments of the present utility model, of the present utility modelly whether to be mixed with in the immuno-chromatographic test paper strip of certain animal flesh for detecting in meat, the antibody of the specific proteins with label of pad absorption and the antibody of the specificity albumen of surveyed area internal fixtion identical or different.
According to specific embodiments of the present utility model, of the present utility modelly whether to be mixed with in the immuno-chromatographic test paper strip of certain animal flesh for detecting in meat, described label is colloidal gold particle, fluorescent microsphere, magnetic microsphere or latex particle, and label particle diameter is 0.02-0.8 μm.
According to specific embodiments of the present utility model, of the present utility modelly whether to be mixed with in the immuno-chromatographic test paper strip of certain animal flesh for detecting in meat, described base plate is the stilt as nitrocellulose filter, thieving paper, sample pad, pad, it can be the PVC hard plate being coated with glue, and wherein the color of PVC base plate comprises the different color such as white and black.
Described by the utility model is immuno-chromatographic test paper strip for detection of adulterations in various meat.In described meat, the sample of detection of adulterations refers to high price meat such as including but not limited to beef or mutton, and the animal category of adulterated meat includes but not limited to pig, duck, chicken, horse, fox, otter etc.
According to a concrete case study on implementation of the present utility model, the specific monoclonal antibody 1 of porcine hemoglobin is for marking collaurum, colloidal gold antibody compound after mark, specking is on glass fibre membrane, then 37 DEG C of dryings 24 hours in air dry oven, then the rectangular of 30cm*0.5cm is cut into, as pad.Another specific monoclonal antibody of porcine hemoglobin is coated on nitrocellulose filter as T line (surveyed area), what C line (control zone) wrapped quilt is sheep anti-mouse igg, wrap by after nitrocellulose filter 37 DEG C of dryings 2 hours in air dry oven.Dried nitrocellulose filter is affixed on PVC base plate, and paste pad, sample pad and thieving paper, the large plate of composition test card, using cutting cutter, that this large plate is cut into 0.4cm is wide, loads getting stuck for the adulterated rapid detection card of pork content in meat of test card.Test card of the present utility model in the detection, meat to be detected is needed to rub meat mud, then include but not limited to that PBS carries out 10-50 and doubly dilutes with damping fluid, 50-150ul is in the sample well of test card in sampling, observes the band shade of detection line and control line after 5-10 minute.T line close to or be deeper than in C line display meat sample and be mixed with pork, T line is obviously shallower than in C line display meat sample does not have pork.
According to another concrete case study on implementation of the present utility model, the specific monoclonal antibody 1 of porcine hemoglobin is for mark fluorescent microballoon, the excitation wavelength of this fluorescent microsphere is 480nm-560nm, fluorescent microsphere antibody complex after mark, specking is on glass fibre membrane, then 37 DEG C of dryings 24 hours in air dry oven, then cut into the rectangular of 30cm*0.5cm, as pad.Another specific monoclonal antibody of porcine hemoglobin is coated on as T line on nitrocellulose filter, C line bag quilt be sheep anti-mouse igg, wrap by after nitrocellulose filter 37 DEG C of dryings 2 hours in air dry oven.Dried nitrocellulose filter is affixed on PVC base plate, and paste pad, sample pad and thieving paper, the large plate of composition test card, using cutting cutter, that this large plate is cut into 0.4cm is wide, loads getting stuck for the adulterated rapid detection card of pork content in meat of test card.Test card of the present utility model in the detection, meat to be detected is needed to rub meat mud, then include but not limited to that PBS carries out 5-100 and doubly dilutes with damping fluid, 50-150ul is in the sample well of test card in sampling, uses fluorescence detector to detect the fluorescence signal of T/C line after 5-10 minute.T line signal is better than in C line display meat sample and is mixed with pork, and T line signal does not have pork lower than in C line display meat sample.
Compared with prior art, the beneficial effects of the utility model are:
1, a kind of field fast detection method of the meat adulteration for different meat specific proteins of the high specific based on antigen-antibody reaction can be provided, the whether adulterated qualification of meat can be completed in 15 minutes, improve the detection efficiency of meat adulteration;
2, the requirement of detection of the present utility model to operation is very low, and layman can realize through simple training;
3, detection of the present utility model does not have substantially for testing laboratory's equipment and facility requirements, greatly improves the possibility that it is promoted in basic unit;
4, testing cost of the present utility model has compared to DNA detection method and reduces greatly;
5, the cost of detection of the present utility model and other requirements, reduce greatly compared to DNA detection method.
In sum, the utility model provides a kind of efficient, cheap, simple meat adulteration detection method, is conducive to the cost that reduction meat adulteration detects, can be used for the unlawful practice hitting meat adulteration effectively, ensure the security of the lives and property of the people.
Accompanying drawing explanation
Fig. 1 is the structural representation of the immunochromatographydetecting detecting test strip of the utility model one specific embodiment.
Fig. 2 is the structural representation of the immunochromatographydetecting detecting test strip that band of the present utility model gets stuck.
Embodiment
Below in conjunction with specific embodiment, the utility model is further described.Described embodiment is intended to specifically illustrate the utility model by way of example, and not forming can the restriction of practical range to the utility model.
The colloidal gold immunochromatographydetection detection test paper bar preparation and property that in embodiment 1 beef, pork is adulterated
Shown in Figure 1, the utility model provides a kind of for detecting the immuno-chromatographic test paper strip whether being mixed with pork in beef, this immuno-chromatographic test paper strip comprises hard floor 101 (PVC hard plate), one deck glue-line 102 is provided with in the upper surface of hard floor 101 is folded, according to liquid chromatography(LC) flowing upstream and downstream direction (in figure from left to right direction), glue-line 102 is pasted with successively sample pad 103, pad 104, porous nitrocellulose film 105 and absorbent filter 106; Wherein:
Described sample pad 103 is fritted glass tunica fibrosa; Its aperture 2-8 μm;
Described pad 104 is the fritted glass tunica fibrosa of absorption labelled antibody, its aperture 2-8 μm, and described labelled antibody is the antibody of the pig specificity albumen with label;
Described porous nitrocellulose film 105 is the porous nitrocellulose film of aperture 2-10 μm, on porous nitrocellulose film 105, vertical liquid chromatographic flow direction is provided with banded surveyed area 501 and control zone 502, surveyed area 501 internal fixtion has the antibody of described pig specificity albumen, and control zone 502 internal fixtion has two of labelled antibody to resist;
Sample pad 103 downstream end is overlapped on the upstream extremity of pad 104, and pad 104 downstream end is overlapped on the upstream extremity of porous nitrocellulose film 105, and the upstream extremity of absorbent filter 106 is overlapped on the downstream end of porous nitrocellulose film 105.
As shown in Figure 2, the immuno-chromatographic test paper strip of the present embodiment also comprises and gets stuck 107, get stuck accommodating hard floor 101, sample pad 103, pad 104, porous nitrocellulose film 105 and absorbent filter 106 in 107, and application of sample window 701 is offered in the position of the 107 upper surface counter sample pads 103 that get stuck, watch window 702 is offered in the corresponding surveyed area of porous nitrocellulose film 105 and the position of control zone.
The test strips of the present embodiment can prepare in accordance with the following methods:
Fire the collaurum that particle diameter is 40nm: 100ml 0.01% chlorauric acid solution boil after add 1% citric acid 1ml, continue to boil to chlorauric acid solution blackening, and then continue to boil 10 minutes, add water supplement fire after collaurum volume to 100ml.Use NaOH that the pH of collaurum is adjusted to 8.3, add the monoclonal antibody A of the anti-porcine hemoglobin of appropriate amount, react 30 minutes; Add the BSA of 5%, react 20 minutes; 16000 turns 30 minutes centrifugal, removing supernatant, redissolves colloid gold label thing with the PB of 0.01M.Use the PBS solution 100 times dilution colloid gold label thing of the 0.01M containing 0.5% sucrose, and with ZX1010 specking platform by colloid gold label thing specking on glass fibre membrane, drying 24 hours in air dry oven; Dried colloid gold label thing is cut into the shape of 30cm*0.5cm, stand-by.
At CN 95 nitrocellulose filter of Sai Duolisi, surveyed area and control zone are set, surveyed area (detection line) bag is by the monoclonal antibody of the anti-porcine hemoglobin of 2mg/ml, control zone (control line) is wrapped by the sheep anti-mouse igg of 1mg/ml, drying 2 hours in air dry oven; Dried nitrocellulose filter is affixed on PVC base plate; Again the colloid gold label thing after cutting is affixed on nitrocellulose filter one end near application of sample window (well), as pad; The glass fibre membrane of 30cm*1.5cm is sticked, as sample pad at the other end of pad; The absorbent filter of 30cm*2cm is sticked, as adsorptive pads at the other end of nitrocellulose filter.Use cutting machine that the large plate of test card after stickup is cut into the wide test strips of 0.4cm, load in getting stuck.The effect supporting whole test strip is played in the bottom of wherein getting stuck, the top of getting stuck, inner in test card for fixing test strips together with bottom; Arrange application of sample window (well) and watch window above getting stuck, application of sample window is used for dripping sample, and by this duct, sample flows on glass fibre membrane, and completes chromatography further; Watch window is for observing the band color of detection line and control line in test card.
The test strips of the present embodiment, when for detecting, can be carried out according to following operation:
Will containing different proportion (pork: beef=P/B, 0%, 1%, 5%, 20%, 50%, 100%) 1g smashed to pieces by the beef of pork, add the PBS 10ml altogether of 0.01M, repeatedly shake mixing, getting supernatant 100ul joins in the well of the adulterated colloidal gold immunochromatographimethod test card of pork, the color depth of detection line and control line is observed after 5 minutes, with the color of detection line close to or be deeper than control line judgement sample for there being pork adulterated, the color of detection line is obviously shallower than control line judgement sample for adulterated without pork.When the pork content content in beef sample is 0% and 1%, the color of detection line is deeper than control line; When the pork content content in beef sample reaches 5%, 20%, 50%, 100%, detection line is equally dark with control line, and namely the sensitivity of the colloidal gold immunochromatographimethod test card that pork is adulterated can reach 5%.
Detection shows, by the colloidal gold immunochromatographimethod test card that pork is adulterated, can detect the adulterated composition of pork in beef by the porcine hemoglobin detected in sample fast high-sensitive degree.
The fluorescence immune chromatography test strip preparation and property that in embodiment 2 beef, pork is adulterated
According to classical way, the monoclonal antibody A of anti-porcine hemoglobin is marked the carboxyl modified fluorescent microsphere (diameter is 0.4um, and excitation/emission wavelength is 470nm and 510nm) of duke: the concentration of dissolving monoclonal antibody A in the MES solution of 50mM is 5mg/ml; The fluorescent microsphere of 2% of 1ml is added, incubated at room temperature 15 minutes in the antibody-solutions of 1ml; Add the EDC of 8mg in this reactant liquor, concussion mixing; Use NaOH to regulate pH to be 6, shake reaction under room temperature 2 hours; Add glycocoll (concentration adjustment is 100mM) cessation reaction 30 minutes; Centrifuging fluorescent microsphere and unreacted antibody.Use containing 0.5% sucrose 0.01M PBS solution 1000 times dilution Fluorescent microsphere marker, and with ZX1010 specking platform by Fluorescent microsphere marker specking on glass fibre membrane, in air dry oven drying 2 hours; Dried Fluorescent microsphere marker is cut into the shape of 30cm*0.5cm, stand-by.
CN 95 nitrocellulose filter of Sai Duolisi arranges surveyed area and the control zone of linear, surveyed area (detection line) bag is by the monoclonal antibody of the anti-porcine hemoglobin of 2mg/ml, control zone (control line) is wrapped by the sheep anti-mouse igg of 1mg/ml, drying 2 hours in air dry oven; Dried nitrocellulose filter is affixed on PVC base plate, then the fluorescence antibody label after cutting is affixed on nitrocellulose filter one end near application of sample window (well), as pad; The glass fibre membrane of 30cm*1.5cm is sticked, as sample pad at the other end of pad; The absorbent filter of 30cm*2cm is sticked, as adsorptive pads at the other end of nitrocellulose filter.Use cutting machine that the large plate of test card after stickup is cut into the wide test strips of 0.4cm, load in getting stuck.The effect supporting whole test strip is played in the bottom of wherein getting stuck, and the top of getting stuck is inner in test card for fixing test strips together with bottom; Arrange application of sample window (well) and watch window above getting stuck, application of sample window is used for dripping sample, and by this duct, sample flows on glass fibre membrane, and completes chromatography further; The fluorescence signal of watch window detection line and control line on induced with laser detector Scanning Detction card.
Will containing different proportion (pork: beef=P/B, 0%, 0.5%, 1%, 5%, 100%) 1g smashed to pieces by the beef of pork, add the PBS 10ml altogether of 0.01M, repeatedly shake mixing, getting supernatant 100ul joins in the sample well of the adulterated fluorescence immune chromatography test card of pork, induced with laser detector is used to scan the fluorescence signal of detection line and control line on nitrocellulose filter film after 10 minutes, with the signal of detection line higher than control line judgement sample for there being pork adulterated, the signal of detection line is adulterated without pork lower than the signal of control line.In the present embodiment, when the pork content content in beef sample reaches 0.5%, the signal of detection line is higher than the signal of control line, and namely the sensitivity of the fluorescence immune chromatography test card that pork is adulterated can reach 0.5%.
Detection shows, by the fluorescence immune chromatography test card that pork is adulterated, can detect the adulterated one-tenth of pork in beef by the porcine hemoglobin detected in sample fast high-sensitive degree.
Claims (9)
1. one kind for detecting the immuno-chromatographic test paper strip whether being mixed with certain animal flesh in meat, it is characterized in that, this immuno-chromatographic test paper strip comprises hard floor, one deck glue-line is provided with in the upper surface of hard floor is folded, according to liquid chromatography(LC) flowing upstream and downstream direction, glue-line is pasted with successively sample pad, pad, porous nitrocellulose film and absorbent filter; Wherein:
Described sample pad is glass fibre membrane or polyester film;
Described pad is the fritted glass tunica fibrosa of absorption labelled antibody, and described labelled antibody is the antibody of the specificity albumen with certain animal described in label;
Described porous nitrocellulose film is the porous nitrocellulose film of aperture 2-10 μm, on porous nitrocellulose film, vertical liquid chromatographic flow direction is provided with banded surveyed area and control zone, surveyed area internal fixtion has the antibody of described specificity albumen, and control zone internal fixtion has two of labelled antibody to resist;
Sample pad downstream end is overlapped on the upstream extremity of pad, and pad downstream end is overlapped on the upstream extremity of porous nitrocellulose film, and the upstream extremity of absorbent filter is overlapped on the downstream end of porous nitrocellulose film.
2. according to claim 1 for detecting the immuno-chromatographic test paper strip whether being mixed with certain animal flesh in meat, it is characterized in that, this immuno-chromatographic test paper strip also comprises and getting stuck, get stuck interior accommodating hard floor, sample pad, pad, porous nitrocellulose film and absorbent filter, and application of sample window is offered in the position of the upper surface counter sample pad that gets stuck, watch window is offered in the corresponding surveyed area of porous nitrocellulose film and the position of control zone.
3. according to claim 1ly it is characterized in that for detecting the immuno-chromatographic test paper strip whether being mixed with certain animal flesh in meat, described sample pad is fritted glass tunica fibrosa or the porous polyester film of aperture 2-8 μm; Described pad is the fritted glass tunica fibrosa of aperture 2-8 μm.
4. according to claim 1ly it is characterized in that for detecting the immuno-chromatographic test paper strip whether being mixed with certain animal flesh in meat, certain animal described is pig, duck, chicken, fox, otter or horse.
5. according to claim 1 or 4 for detecting the immuno-chromatographic test paper strip whether being mixed with certain animal flesh in meat, it is characterized in that, described specificity albumen is immunoglobulin (Ig) or seralbumin or troponin.
6. according to claim 1 for detecting the immuno-chromatographic test paper strip whether being mixed with certain animal flesh in meat, it is characterized in that, the antibody of the antibody of the specific proteins with label that pad adsorbs and the specificity albumen of surveyed area internal fixtion is identical or different.
7. according to claim 1ly it is characterized in that for detecting the immuno-chromatographic test paper strip whether being mixed with certain animal flesh in meat, described label is colloidal gold particle, fluorescent microsphere, magnetic microsphere or latex particle, and label particle diameter is 0.02-0.8 μm.
8. according to claim 1ly it is characterized in that for detecting the immuno-chromatographic test paper strip whether being mixed with certain animal flesh in meat, two of described labelled antibody resists for sheep anti-mouse igg.
9. according to claim 1ly it is characterized in that for detecting the immuno-chromatographic test paper strip whether being mixed with certain animal flesh in meat, described hard floor is PVC hard plate.
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| CN104880563A (en) * | 2015-04-08 | 2015-09-02 | 上海盛复源生物医药有限公司 | NGAL rapid detection reagent and preparation method thereof |
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| CN106596955A (en) * | 2016-12-13 | 2017-04-26 | 辽宁省药品检验检测院 | Rapid detection kit for pork, beef and mutton and preparation method of kit |
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| CN108427006A (en) * | 2018-05-16 | 2018-08-21 | 吉林吉和迅生物技术有限公司 | A kind of colloidal gold fast detecting test paper card |
| CN111537723A (en) * | 2020-05-12 | 2020-08-14 | 北京勤邦生物技术有限公司 | Test strip and method for detecting chicken doped in beef and mutton |
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| CN104914250A (en) * | 2015-06-10 | 2015-09-16 | 长沙安迪生物科技有限公司 | Rapid mutton detection card and detection method thereof |
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| CN108051589A (en) * | 2017-12-06 | 2018-05-18 | 扬州大学 | A kind of rapid detection card for differentiating pilose antler Blood piece |
| CN108427006A (en) * | 2018-05-16 | 2018-08-21 | 吉林吉和迅生物技术有限公司 | A kind of colloidal gold fast detecting test paper card |
| CN112740039A (en) * | 2018-09-27 | 2021-04-30 | 积水医疗株式会社 | Immunochromatographic test piece |
| CN111537723A (en) * | 2020-05-12 | 2020-08-14 | 北京勤邦生物技术有限公司 | Test strip and method for detecting chicken doped in beef and mutton |
| CN112946252A (en) * | 2021-02-01 | 2021-06-11 | 福州海关技术中心 | Fluorescent test strip for identifying duck meat doped in beef and mutton and preparation method and application thereof |
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