CN202049158U - Immunofluorescence test paper strip - Google Patents
Immunofluorescence test paper strip Download PDFInfo
- Publication number
- CN202049158U CN202049158U CN 201020650783 CN201020650783U CN202049158U CN 202049158 U CN202049158 U CN 202049158U CN 201020650783 CN201020650783 CN 201020650783 CN 201020650783 U CN201020650783 U CN 201020650783U CN 202049158 U CN202049158 U CN 202049158U
- Authority
- CN
- China
- Prior art keywords
- fixed
- antibody
- test strips
- antigen
- strip
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Images
Abstract
The utility model discloses an immunofluorescence test paper strip, which consists of a sample pad, a reaction membrane and an absorbent pad, wherein the sample pad, the reaction membrane and the absorbent pad are mutually sequentially overlapped on a casing. An internal quality control strip, at least one detecting strip and an over-dose antigen detecting strip are fixed on the reaction membrane, quantitative antigen or antibody substance serving as internal reference is fixed on the internal quality control strip, antibodies in specific binding with substance to be detected is fixed on the detecting strips, and a secondary antibody is fixed on the over-dose antigen detecting strip. The immunofluorescence test paper strip can be used for ration detection of various clinical diagnostic biomarkers, specific biological molecules, pesticide residues, microorganism, water pollutants and the like, has the advantages of convenience, speediness, portability, safety and nontoxicity of reagent and few environment pollutions, and is particularly suitable for the field of external speedy diagnose and detection.
Description
Technical field
The utility model relates generally to the fluorescence immune chromatography detection range, belongs to a kind of of dry chemical detection method, is specifically related to a kind of immunofluorescence test strips.
Background technology
The fluoroimmunoassay technology is a kind of analytical technology based on antigen-antibody reaction that grows up gradually in immuno-labelling technique.Its birth early stage, some scholars attempt antibody molecule is combined with some probe materials, utilize antigen-antibody reaction to organize or the location of intracellular antigen material.Finally by Coons etc., first fluorescein is used for the mark of antibody and succeeds in nineteen forty-one.This technology of carrying out Antigen Location with the fluorescent material labelled antibody is called fluorescent antibody technics (fluorescent antibody technique).
Use the fluoroimmunoassay technology can be used for detecting antigen, also can be used to detect antibody.The method that wherein is used to detect antigen is comparatively commonly used, is commonly referred to fluorescence anti-body method.Antibody is carried out being used to check that methods such as cell or tissue endoantigen or haptens material are called immunofluorescence cell (or tissue) chemical technology behind the fluorescence labeling.
Different with traditional enzyme linked immunological absorption (ELISA) method, when application fluoroimmunoassay technology detected, its signal specificity was strong, susceptibility is high, speed is very fast, is particularly suitable for external quick diagnosis field.Though also there is the deficiency of aspects such as unspecific staining.But along with the preparation success of high-quality antibody, the particularly appearance of the genetic engineering antibody that specificity is higher, single-chain antibody etc., its not enough aspect has obtained good improvement.And reagent safety is nontoxic, environment and operator is damaged little etc., therefore, is subjected to more and more clinical examination workers' favor.
The dry analysis method be meant with traditional liquor analysis comparatively speaking, be about to required analytical reagent and all be solidificated on the multilayer complex films, only a small amount of sample to be checked (a few microlitre) need be added on the film during analysis and can carry out qualitative or quantitative test.
Immunochromatography technique is said in a broad sense, also belongs to a kind of of dry chemical.Use nitrocellulose filters etc. utilize the porous effect of film to react as the carrier that reactant supports.This technology can be divided into two kinds of longitudinal diffusion and transverse dispersion.What be born in early days is the spot gold hybrid method of longitudinal diffusion, promotes the use of but fail to become main flow reagent because of its complicated operation eventually.Along with the development of membrane technology, lateral flow transverse dispersion test strips demonstrates good advantage, and is promoted greatly.Most widely used collaurum fast diagnose test paper is used for the detection of very early pregnancy (HCG) in the lateral flow immunochromatography technique.Because it is convenient, fast, specificity is high, is particularly suitable for care diagnostic, therefore, its application is very extensive, becomes the mainstream product in the lateral flow immunochromatography technique.Yet, this series products often only can be used for the primary dcreening operation of sample, and its major defect is that detection sensitivity is lower, can only be used for observational measurement or sxemiquantitative and measure, though also can carry out interpretation by certain instrument, but still can't carry out quantitative measurment to the depth of colour developing band.And the result who detects is difficult for record and preserves.
The utility model content
In order to overcome deficiency of the prior art, the purpose of this utility model is to provide a kind of immunofluorescence test strips, use the utility model can realize, and can realize the joint-detection of unitem or a plurality of projects biomacromolecule or micromolecular detection by quantitative such as various blood serum designated objects, microbial antigen, virion, illegal drugs.Another purpose of the present utility model is to provide a kind of quantitative detecting method of immunofluorescence test strips.
In order to solve the problems of the technologies described above, realize above-mentioned purpose, the utility model is achieved through the following technical solutions:
A kind of immunofluorescence test strips, sample pad, reaction film and the absorption pad of overlap joint are formed mutually on the shell by being successively set on, be fixed with an inner quality control band on the described reaction film, at least one detection band and an excessive Detection of antigen band, wherein inner quality control band is fixed with quantitative antigen or antibody materials, detect band and be fixed with the antibody that can combine with the test substance specificity, excessive Detection of antigen band is fixed with two and resists.
Preferably, the material of described shell can be any one among ABS, PS, PE, PVC or the PC.
Preferably, the material of described sample pad can be any one in cellulose, glass fibre or the textile polymer.
Preferably, the material of described reaction film can be any one in nitrocellulose filter, cellulose acetate membrane, nylon membrane or the poly tetrafluoroethylene.
Preferably, absorption pad can be any one in the plain mixture of cellulose, glass fibre element or cellulose glass fibre.
A kind of immunofluorescence test strips quantitative detecting method may further comprise the steps:
The liquid that step 1. will contain the target detection thing joins in the fluorescence reaction reagent, and reaction 1min;
Step 2. is got quantitative reaction reagent and is dripped on the sample pad (2) of immunofluorescence test strips of the present utility model, carries out immune response, and the described quantitative value of reaction reagent is enough participated in immune response and got final product;
Behind the step 3. reaction 3min, be placed on the fluorescence analysis detecting device, fluorescence signal intensity is read.
Further, the inside of described fluorescence reaction reagent comprises two or more and is used for quantitative labelled antibody, and the fluorescent material of labelled antibody can produce the material of fluorescence in directly fluorescent dye, latex particle, quantum dot or the magnetic nanoparticle any one.
The utility model can use laser, halogen tungsten lamp+monochromator or high-capacity LED as light source when test strip, excites the fluorescent material on the test strips to send fluorescence.
The reading device of fluorescence signal can use photodiode, CCD or photomultiplier that signal is read in the utility model.
The utility model carries out determined antigen when quantitative, uses the Processing Algorithm based on fluorescence signal intensity on the reaction film.Mainly being based on the maximal value of fluorescence signal or the area of fluorescence signal calculates.Fluorescence signal mainly contains following several situation:
(1) no determined antigen
(2) determined antigen concentration is no more than sensing range
(3) determined antigen concentration is higher than maximum detectability
Compared with prior art, the utility model is by the power analysis to fluorescence signal on the test strips, and the data processing algorithm of combination optimization, can realize the accurate quantification of target detection thing is detected.Immunofluorescence test strips of the present utility model can be used for the detection by quantitative of various clinical diagnosis biomarkers, specific biological molecule, pesticide residue, microorganism, water pollutant etc.Have convenient and swift, be easy to carry about with one, reagent safety is nontoxic, environmental pollution is little characteristics, be particularly suitable for external quick diagnosis and fast detecting field.
Above-mentioned explanation only is the general introduction of technical solutions of the utility model, for can clearer understanding technological means of the present utility model, and can be implemented according to the content of instructions, below with preferred embodiment of the present utility model and conjunction with figs. describe in detail as after.Embodiment of the present utility model is provided in detail by following examples and accompanying drawing thereof.
Description of drawings
Below in conjunction with drawings and embodiments the utility model is described in further detail.
Fig. 1 is the structural representation of immunofluorescence test strips of the present utility model.
Number in the figure explanation: 1, shell, 2, sample pad, 3, inner quality control band, 4, detect band, 5, excessive Detection of antigen band, 6, reaction film, 7, absorption pad.
Embodiment
Referring to shown in Figure 1, a kind of immunofluorescence test strips, sample pad 2, reaction film 6 and the absorption pad 7 of overlap joint are formed mutually on the shell 1 by being successively set on, be fixed with an inner quality control band 3 on the described reaction film 6,4 and excessive Detection of antigen bands 5 are with at least one detection, wherein inner quality control band 3 is fixed with quantitative antigen or antibody materials, and detection is fixed with the antibody that can combine with the test substance specificity with 4, and excessive Detection of antigen band 5 is fixed with two and resists.
Embodiment 1: the detection of cardiac muscle troponin I (cTnI)
(1) preparation of test strips:
1) preparation of reaction film
Choose Millipore HF180 tape backing film as reaction film.With film to be cut into 30 * 1.8cm size standby.Goat anti-rabbit igg is used 0.01M, PH be 7.2 PBS to regulate its concentration be 0.5mg/mL, add 2% or 1% Tween-20, use and draw a film instrument it is sprayed on NC film surface as inner nature controlling line, drawing a film amount is every centimetre 0.5 μ L.No. 1 cTnI antibody-solutions is used 0.01M, PH be 7.2 PBS to regulate its concentration be 0.5mg/mL, add 2% or 1% Tween-20, use and draw a film instrument it is sprayed on NC film surface, drawing a film amount is every centimetre 0.5 μ L, as detection line.With sheep anti mouse I gG antibody-solutions use 0.01M, PH be 7.2 PBS to regulate its concentration be 0.5mg/mL, add 2% or 1% Tween-20, use and draw a film instrument it is sprayed on NC film surface, drawing a film amount is every centimetre 0.5 μ L, as excessive Detection of antigen line.Be spaced apart 4mm between per two lines.Draw and put into 37 degree baking oven dried overnight after film finishes immediately, the drying at room temperature condition is preserved standby.
2) assembling of test strips
Choose homemade high-quality glass fibre membrane BT100 as the sample pad material, it is standby that it is cut into 30 * 2cm size.Choosing homemade high-quality thieving paper CH37K, that it is cut into 30 * 1.8cm size is standby.
Be at first to paste reaction film on the PVC base plate of 30 * 6cm in specification, the method that sample pad and thieving paper are taked to overlap sticks on the base plate then, and the length of overlap joint is 0.5mm, so that the diffusion of reactant liquor.After assembling, adopt special slitting knife, test strips is cut into the wide test strips of 4mm, and in encapsulation and the plastic casing, be put in the middle of the aluminium foil, 4 ℃ of sealings are preserved.
3) preparation of fluorescence reaction liquid
Use PBS to regulate its concentration No. 2 cTnI antibody-solutions and be 2mg/mL, PH is between the 7.5-8.0, get the 1mL antibody-solutions, slowly adding concentration is the cy5-se dyestuff 100 μ L of 1mg/mL, 4 ℃ of slow stirring reaction 3h, normal temperature continue reaction 1h down, after question response is complete, using PH is that 7.4 PBS dialyses, and removes unreacted cy5-se.During beginning, change dislysate one time, change that every 12h changes dislysate one time after 2 times, after the dialysis fully, reclaim the good fluorescence antibody of coupling every 4h, measure coupling efficiency and antibody concentration after, keep in Dark Place.
The rabbit igg use is prepared identical method with cardiac troponin albumen fluorescence antibody, prepare the rabbit igg that is marked with cy5, it is standby to keep in Dark Place.
The cTnI fluorescence antibody 1mL that gets 1mg/mL mixes with the 1mg/mL rabbit igg fluorescence antibody of 0.1mL, and using 0.01M, PH is 10 times of 7.4 PBS dilutions, and adds 1% Tween-20,0.1%BSA, 0.1%NaN
3Mix mutually, preparation feedback liquid, the per 200 μ L of reactant liquor are a pipe, carry out packing, 4 ℃ keep in Dark Place standby.
(2) testing procedure
1) with test strips and reactant liquor by taking out in the packaging bag, and be put in more than the equilibrium at room temperature 10min;
2) get fresh whole blood 20 μ L (or serum 10 μ L), join and divide in the fluorescence reaction liquid (200 μ L/ pipe) that installs, reaction 1min; The centre shakes up gently;
3) the reactant liquor potpourri is added on the sample pad, make reaction mixture on test strips, carry out the lateral chromatography immune response; Time is 3min;
4) after reaction finishes, test strips is put in special fluorescence signal reads in the instrument, read the size of fluorescence signal, carry out quantitative measurement.
The detection of embodiment 2:C-reactive protein (CRP)
(1) preparation of test strips:
1) preparation of reaction film
Choose Millipore HF180 tape backing film as reaction film.With film to be cut into 30 * 1.8cm size standby.With goat-anti rabbit I gG use 0.01M, PH be 7.2 PBS to regulate its concentration be 0.5mg/mL, add 2% or 1% Tween-20, use and draw a film instrument it is sprayed on NC film surface as inner nature controlling line, drawing a film amount is every centimetre 0.5 μ L.No. 1 CRP antibody-solutions is used 0.01M, PH be 7.2 PBS to regulate its concentration be 0.5mg/mL, add 2% or 1% Tween-20, use and draw a film instrument it is sprayed on NC film surface, drawing a film amount is every centimetre 0.5 μ L, as detection line.With sheep anti mouse I gG antibody-solutions use 0.01M, PH be 7.2 PBS to regulate its concentration be 0.5mg/mL, add 2% or 1% Tween-20, use and draw a film instrument it is sprayed on NC film surface, drawing a film amount is every centimetre 0.5 μ L, as excessive Detection of antigen line.Be spaced apart 4mm between per two lines.Draw and put into 37 degree baking oven dried overnight after film finishes immediately, the drying at room temperature condition is preserved standby.
2) assembling of test strips
Choose homemade high-quality glass fibre membrane BT100 as the sample pad material, it is standby that it is cut into 30 * 2cm size.Choosing homemade high-quality thieving paper CH37K, that it is cut into 30 * 1.8cm size is standby.
Be at first to paste reaction film on the PVC base plate of 30 * 6cm in specification, the method that sample pad and thieving paper are taked to overlap sticks on the base plate then, and the length of overlap joint is 0.5mm, so that the diffusion of reactant liquor.After assembling, adopt special slitting knife, test strips is cut into the wide test strips of 4mm, and in encapsulation and the plastic casing, be put in the middle of the aluminium foil, 4 ℃ of sealings are preserved.
3) preparation of fluorescence reaction liquid
Use PBS to regulate its concentration No. 2 CRP antibody-solutions and be 3mg/mL, PH is between the 7.5-8.0, get the 1mL antibody-solutions, slowly adding concentration is the cy5-se dyestuff 100 μ L of 1mg/mL, 4 ℃ of slow stirring reaction 3h, normal temperature continue reaction 1h down, after question response is complete, using PH is that 7.4 PBS dialyses, and removes unreacted cy5-se.During beginning, change dislysate one time, change that every 12h changes dislysate one time after 2 times, after the dialysis fully, reclaim the good fluorescence antibody of coupling every 4h, measure coupling efficiency and antibody concentration after, keep in Dark Place.
Rabbit igg uses and prepares identical method with cardiac troponin albumen fluorescence antibody, prepares the rabbit igg that is marked with cy5, and it is standby to keep in Dark Place.
The cTnI fluorescence antibody 1mL that gets 1mg/mL mixes with the 1mg/mL rabbit igg fluorescence antibody of 0.1mL, use 0.01M, PH is 10 times of 7.4 PBS dilutions, and add 1% Tween-20, and 0.1%BSA, 0.1%NaN3 mixes mutually, preparation feedback liquid, the per 200 μ L of reactant liquor are a pipe, carry out packing, and 4 ℃ keep in Dark Place standby.
(2) testing procedure
1) with test strips and reactant liquor by taking out in the packaging bag, and be put in more than the equilibrium at room temperature 10min;
2) get fresh whole blood 20 μ L (or serum 10 μ L), join and divide in the fluorescence reaction liquid (200 μ L/ pipe) that installs, reaction 1min; The centre shakes up gently;
3) the reactant liquor potpourri is added on the sample pad, make reaction mixture on test strips, carry out the lateral chromatography immune response; Time is 3min;
4) after reaction finishes, test strips is put in special fluorescence signal reads in the instrument, read the size of fluorescence signal, carry out quantitative measurement.
The foregoing description just is to allow the one of ordinary skilled in the art can understand content of the present utility model and enforcement according to this for technical conceive of the present utility model and characteristics being described, its objective is, can not limit protection domain of the present utility model with this.The variation or the modification of every equivalence of having done according to the essence of the utility model content all should be encompassed in the protection domain of the present utility model.
Claims (5)
1. immunofluorescence test strips, upward sample pad (2), reaction film (6) and the absorption pad (7) of overlap joint are formed mutually by being successively set on shell (1), it is characterized in that: be fixed with an inner quality control band (3) on the described reaction film (6), a detection band (4) of at least one and an excessive Detection of antigen band (5), wherein inner quality control band (3) is fixed with quantitative antigen or antibody materials, detect band (4) and be fixed with the antibody that can combine with the test substance specificity, excessive Detection of antigen band (5) is fixed with two and resists.
2. immunofluorescence test strips according to claim 1 is characterized in that: the material of described shell (1) can be any one among ABS, PS, PE, PVC or the PC.
3. immunofluorescence test strips according to claim 1 is characterized in that: the material of described sample pad (2) can be any one in cellulose, glass fibre or the textile polymer.
4. immunofluorescence test strips according to claim 1 is characterized in that: the material of described reaction film (6) can be any one in nitrocellulose filter, cellulose acetate membrane, nylon membrane or the poly tetrafluoroethylene.
5. immunofluorescence test strips according to claim 1 is characterized in that: absorption pad (7) can be any one in cellulose or the glass fibre element.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201020650783 CN202049158U (en) | 2010-12-09 | 2010-12-09 | Immunofluorescence test paper strip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201020650783 CN202049158U (en) | 2010-12-09 | 2010-12-09 | Immunofluorescence test paper strip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202049158U true CN202049158U (en) | 2011-11-23 |
Family
ID=44989463
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201020650783 Expired - Lifetime CN202049158U (en) | 2010-12-09 | 2010-12-09 | Immunofluorescence test paper strip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202049158U (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102520193A (en) * | 2011-12-29 | 2012-06-27 | 深圳康美生物科技股份有限公司 | Fluorescence immunochromatographic assay and kit for quantitative detection of human cardiac troponin I (cTnI) |
| CN102539751A (en) * | 2010-12-09 | 2012-07-04 | 苏州生物医学工程技术研究所 | Immunofluorescence test paper strip and quantitative detection method thereof |
| CN102680703A (en) * | 2012-04-28 | 2012-09-19 | 广州鸿琪光学仪器科技有限公司 | Immunofluorescence test strip component for quickly and quantitatively testing myoglobin, test card component using immunofluorescence test strip component, and preparation method for immunofluorescence test strip component |
| CN102680702A (en) * | 2012-04-28 | 2012-09-19 | 广州鸿琪光学仪器科技有限公司 | Immune-fluorescence test strip component for rapidly detecting C-reactive protein quantitatively, detection card component produced by same and method for preparing same |
| CN102680704A (en) * | 2012-04-28 | 2012-09-19 | 广州鸿琪光学仪器科技有限公司 | immunofluorescence test strip module for rapidly and quantitatively testing microalbumin, test card module made of same and preparation method thereof |
| CN103558378A (en) * | 2013-11-07 | 2014-02-05 | 李鑫 | Rapid detection kit for tomato ringspot viruses |
| CN103954753A (en) * | 2014-05-12 | 2014-07-30 | 中国科学院苏州生物医学工程技术研究所 | Quantitative determination method of immune chromatography test strip |
-
2010
- 2010-12-09 CN CN 201020650783 patent/CN202049158U/en not_active Expired - Lifetime
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102539751A (en) * | 2010-12-09 | 2012-07-04 | 苏州生物医学工程技术研究所 | Immunofluorescence test paper strip and quantitative detection method thereof |
| CN102520193A (en) * | 2011-12-29 | 2012-06-27 | 深圳康美生物科技股份有限公司 | Fluorescence immunochromatographic assay and kit for quantitative detection of human cardiac troponin I (cTnI) |
| CN102520193B (en) * | 2011-12-29 | 2014-08-13 | 深圳康美生物科技股份有限公司 | Fluorescence immunochromatographic assay and kit for quantitative detection of human cardiac troponin I (cTnI) |
| CN102680703A (en) * | 2012-04-28 | 2012-09-19 | 广州鸿琪光学仪器科技有限公司 | Immunofluorescence test strip component for quickly and quantitatively testing myoglobin, test card component using immunofluorescence test strip component, and preparation method for immunofluorescence test strip component |
| CN102680702A (en) * | 2012-04-28 | 2012-09-19 | 广州鸿琪光学仪器科技有限公司 | Immune-fluorescence test strip component for rapidly detecting C-reactive protein quantitatively, detection card component produced by same and method for preparing same |
| CN102680704A (en) * | 2012-04-28 | 2012-09-19 | 广州鸿琪光学仪器科技有限公司 | immunofluorescence test strip module for rapidly and quantitatively testing microalbumin, test card module made of same and preparation method thereof |
| CN102680703B (en) * | 2012-04-28 | 2014-12-17 | 广州鸿琪光学仪器科技有限公司 | Immunofluorescence test strip component for quickly and quantitatively testing myoglobin, test card component using immunofluorescence test strip component, and preparation method for immunofluorescence test strip component |
| CN102680702B (en) * | 2012-04-28 | 2014-12-17 | 广州鸿琪光学仪器科技有限公司 | Immune-fluorescence test strip component for rapidly detecting C-reactive protein quantitatively, detection card component produced by same and method for preparing same |
| CN102680704B (en) * | 2012-04-28 | 2015-02-11 | 广州鸿琪光学仪器科技有限公司 | immunofluorescence test strip module for rapidly and quantitatively testing microalbumin, test card module made of same and preparation method thereof |
| CN103558378A (en) * | 2013-11-07 | 2014-02-05 | 李鑫 | Rapid detection kit for tomato ringspot viruses |
| CN103954753A (en) * | 2014-05-12 | 2014-07-30 | 中国科学院苏州生物医学工程技术研究所 | Quantitative determination method of immune chromatography test strip |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN202049158U (en) | Immunofluorescence test paper strip | |
| CN102539751A (en) | Immunofluorescence test paper strip and quantitative detection method thereof | |
| CN106872420B (en) | Kit and method for time-resolved fluorescence quantitative detection of microalbuminuria | |
| CN102680692B (en) | Immunochromatographic quantitative test reagent based on near-infrared fluorescent marker | |
| Wang et al. | Novel fabrication of immunochromatographic assay based on up conversion phosphors for sensitive detection of clenbuterol | |
| CN105823880B (en) | A kind of utilization hook effect expands the biochip and its detection method of detection range | |
| CN101339196A (en) | Rapid checking method for bladder cancer by quantum dot mark immunity-chromatography test paper | |
| CN104090248A (en) | Reagent for quantitative detection of Beta-receptor stimulant through Europium chelate latex time-resolved immunochromatographic assay | |
| CN103197074A (en) | Immunochromatography quantitative determination reagent based on near infrared fluorescence nanoparticle markers | |
| JPH0464062A (en) | Method of detecting presence of antigen and device used for said method | |
| CN202676699U (en) | Special fluorescent-labeled immunity quantitative detection test strip | |
| CN207248894U (en) | Bladder chalone C time resolution detection card and kit | |
| CN103940798A (en) | Solid fluorescent nanometer microsphere as well as preparation method and application thereof | |
| CN108535485A (en) | A kind of time-resolved fluoroimmunoassay chromatograph test strip and preparation method quantitatively detecting CA153 in blood | |
| KR101718485B1 (en) | Device for Detecting Colored Reaction or Fluorescence Reaction of Immunochromatography | |
| CN101566631A (en) | Magnetic immunochromatographic test strip for combined detection of HIV-1+2 antibody and P24 antigen and preparation method thereof | |
| CN101762700A (en) | Magnetic immuno-chromatographic test paper strip for quantitatively detecting carcinoembryonic antigen in blood and preparation method thereof | |
| CN101566636A (en) | Magnetic immunochromatographic test strip for quantitatively detecting alpha-fetoprotein in blood and preparation method thereof | |
| CN101762699A (en) | Magnetic immuno-chromatographic test paper strip for quantitatively detecting tumor associated antigen 125 in blood and preparation method thereof | |
| TW494238B (en) | Chromatographic test pieces and chromatographic assay | |
| CN104215757B (en) | Biological fluid sample quantitative test device and its detection method | |
| WO2022042320A1 (en) | Ultra-sensitive digital rapid chromatographic detection system and method for analyte | |
| CN208367017U (en) | Serum amyloid A protein immunochromatographiassay assay quantitative detection test paper | |
| CN106645043A (en) | Kit and method for fast quantitatively detecting small molecule compound | |
| CN106526166A (en) | Rapid detection of lean meat powder in pork |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20111123 |