CN114878818A - Test strip for detecting pathogenic bacteria and pH value in drinking water and preparation method thereof - Google Patents
Test strip for detecting pathogenic bacteria and pH value in drinking water and preparation method thereof Download PDFInfo
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- CN114878818A CN114878818A CN202210661822.9A CN202210661822A CN114878818A CN 114878818 A CN114878818 A CN 114878818A CN 202210661822 A CN202210661822 A CN 202210661822A CN 114878818 A CN114878818 A CN 114878818A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54386—Analytical elements
- G01N33/54387—Immunochromatographic test strips
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
- G01N33/56916—Enterobacteria, e.g. shigella, salmonella, klebsiella, serratia
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- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/21—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Pseudomonadaceae (F)
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Abstract
The invention discloses a test strip for detecting pathogenic bacteria and pH value in drinking water, which comprises a first shell, a fifth slot and a test strip main body, wherein a second shell is clamped on the first shell, a pH colorimetric card is adhered on the second shell, a circular groove is formed in the second shell, a first slot is formed in the right side of the circular groove, a second slot is formed in the right side of the first slot, a third slot is formed in the right side of the second slot, and a fourth slot is formed in the right side of the third slot. According to the test strip for detecting pathogenic bacteria and pH value in drinking water and the preparation method thereof, three common aquatic microorganism pathogenic bacteria can be detected simultaneously, the water absorption filter paper of the conventional colloidal gold test strip is replaced by the pH test strip, the pH value of the drinking water is synchronously tested, the device is more economical and practical in practical application due to the mode, the operation is simple, the identification is quick, and unnecessary resource waste and time and labor are reduced.
Description
Technical Field
The invention relates to the technical field of colloidal gold detection, in particular to a test strip for detecting pathogenic bacteria and pH value in drinking water and a preparation method thereof.
Background
The microorganisms are the primary problem threatening the safety of drinking water, pathogenic bacteria such as Escherichia coli, pseudomonas aeruginosa, clostridium perfringens and the like are often easily detected in the drinking water of people, the water quality safety of the drinking water is directly related to the body health of residents, and once the drinking water is polluted, the water quality safety of the drinking water can greatly threaten the life safety of human beings. The detection and identification of microorganisms by traditional microbial culture and biochemical methods are common methods in daily detection, and have the disadvantages of complicated operation and long time. The development and application of the water environment microorganism rapid detection technology are guarantees for promoting the rapid detection of microorganisms in drinking water sources and the development of water quality safety early warning technology. With the new requirements for rapid detection and accurate early warning of water quality microbial pollution, online detection and early warning technologies for microbes in water environments are increasingly developed and applied. The quick microbial detection method is to be popularized and applied in the water quality detection industry as soon as possible, and the immune colloidal gold test paper detection method is to detect the escherichia coli in food by using an antigen-antibody immune labeling technology according to the actual condition of water quality detection. The carrier of the immune colloidal gold is nitrocellulose, a sample is dripped into the micropore, liquid dripped at one end of the membrane slowly moves to the other end under the capillary action, and the immune colloidal gold is combined by an antigen and an antibody and is used for presenting a color reaction to judge whether the sample is polluted by escherichia coli.
However, the detection test paper for drinking water detection on the market at present cannot meet the requirements of people due to relatively imperfect technology, the prior art can only realize the colloidal gold detection of single flora escherichia coli in food, and the mode ensures that the corresponding detection test paper is needed when other microorganisms and pH value detection are carried out, thereby greatly improving the detection cost and simultaneously causing unnecessary resource waste. Therefore, a test strip for simultaneously detecting three common pathogenic bacteria and pH value in drinking water and a preparation method thereof are provided, so as to solve the problems provided in the above.
Disclosure of Invention
The invention aims to provide a test strip for detecting pathogenic bacteria and pH value in drinking water and a preparation method thereof, and aims to solve the problems that the prior test strip for detecting drinking water in the prior art cannot meet the requirements of people due to relatively imperfect technology, the prior art can only realize the colloidal gold detection of escherichia coli in a single flora in food, and the mode needs to adopt corresponding test strips when other microorganisms and pH value are detected, so that the detection cost is greatly improved, and unnecessary resource waste is caused.
In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a detect test paper strip of pathogenic bacterium and pH value in drinking water, includes first casing, fifth fluting and test paper strip main part, the block has the second casing on the first casing, and pastes on the second casing and have pH colour comparison card, circular recess has been seted up on the second casing, and the right side of circular recess is provided with first fluting, the second fluting has been seted up on first grooved right side, and the grooved right side of second is provided with the third fluting, the fourth fluting has been seted up on the grooved right side of third, fourth fluting right side is provided with the fifth fluting, install the test paper strip main part between first casing and the second casing.
Preferably, the test strip main body comprises a PVC bottom plate, a nitrocellulose membrane, a first score, a second score, a third score, a fourth score, a colloidal gold pad, a sample pad and a pH test strip.
Preferably, a nitrocellulose membrane is attached to the PVC base plate, a first scribing line is arranged on the nitrocellulose membrane, the position of the first scribing line corresponds to the position of the first groove, a second scribing line is arranged on the nitrocellulose membrane, the position of the second scribing line corresponds to the position of the second groove, a third scribing line is arranged on the nitrocellulose membrane, the position of the third scribing line corresponds to the position of the third groove, a fourth scribing line is arranged on the nitrocellulose membrane, the position of the fourth scribing line corresponds to the position of the fourth groove, a colloidal gold pad is attached to the nitrocellulose membrane, a sample pad is attached to the PVC base plate and the colloidal gold pad, a pH test paper is attached to the PVC base plate and the nitrocellulose membrane, and a fifth groove is arranged right above the pH test paper.
Preferably, the first slot, the second slot, the third slot, the fourth slot and the fifth slot have the same opening size, and the first slot, the second slot, the third slot, the fourth slot and the fifth slot are distributed in an equidistant array.
Preferably, the length of the test strip main body is 10cm, and the width of the test strip main body is 0.5 cm.
A preparation method of a test strip for detecting pathogenic bacteria and pH value in drinking water sequentially comprises the following steps:
s1 preparation and treatment steps of colloidal gold: firstly, adding 50ml of purified water and 2ml of chloroauric acid into a clean beaker, then heating a mixture of the purified water and the chloroauric acid, heating to boil, adding 250ml of trisodium citrate solution at one time, turning off a heating device after the trisodium citrate solution is added and boiled for 10min, adding deionized water into the material after the heated material is cooled until the total mass of the material is 50g, and uniformly stirring the material and the deionized water for later use;
s2 preparation of colloidal gold label: taking 1.5ml of the material prepared in the step S1, adding 9ul of potassium carbonate solution into the material, simultaneously carrying out rapid homogenization treatment, then sequentially adding 15ug of escherichia coli antibody, 15ug of pseudomonas aeruginosa antibody and 15ug of clostridium perfringens antibody, mixing uniformly, standing for 10min, adding 15ul of confining liquid, mixing uniformly, standing for 10min, carrying out centrifugal treatment by a centrifuge, removing clear liquid, re-dissolving by using colloidal gold re-dissolving solution to 0.5ml, and then fully homogenizing the solution by using ultrasonic waves to obtain a colloidal gold marker;
s3 preparation of the colloidal gold pad: cutting the glass cellulose membrane, then placing the colloidal gold on the cut glass cellulose membrane, uniformly drying by adopting drying equipment, and cutting the formed colloidal gold pad into the width size of 0.5cm after drying is finished;
antibody coating step on S4 nitrocellulose membrane: sucking 10 mu l of escherichia coli antibody by a pipette to be matched with a ruler to perform scribing on a first scribing position on the nitrocellulose membrane, sucking 10 mu l of pseudomonas aeruginosa antibody by the pipette to be matched with the ruler to perform scribing on a second scribing position on the nitrocellulose membrane, sucking 10 mu l of clostridium perfringens antibody by the pipette to be matched with the ruler to perform scribing on a third scribing position on the nitrocellulose membrane, and sucking 10 mu l of goat anti-rabbit secondary antibody by the pipette to be matched with the ruler to perform scribing on a fourth scribing position on the nitrocellulose membrane;
s5 test paper assembly preparation: the nitrocellulose membrane with the scribed lines in the S4 is attached to a PVC base plate, then the right end of a colloidal gold pad is attached to the left end of the nitrocellulose membrane, a sample pad is attached to the colloidal gold pad, pH test paper is attached to the right end of the nitrocellulose membrane, the assembled test paper is cut by a cutting device to be 10cm in length, the width of the test paper is 0.5cm, the test paper is placed inside a first shell and a second shell, and the pH colorimetric card is attached to the second shell to complete the whole assembly.
Preferably, the rotation speed of the centrifuge in step S2 is 9000rpm, and the centrifugation time is controlled at 7 min.
Preferably, the length and width dimensions of the glass cellulose film after being slit in step S3 are both 5 cm.
Compared with the prior art, the test strip for detecting pathogenic bacteria and pH value in drinking water and the preparation method thereof have the advantages that:
through immune colloidal gold test paper detection method to fuse the detection of three kinds of common aquatic microorganism pathogenic bacteria into same test paper simultaneously, make this technical scheme can detect three kinds of common aquatic microorganism pathogenic bacteria simultaneously: pathogenic bacteria such as escherichia coli, pseudomonas aeruginosa, clostridium perfringens and the like, and the water-absorbing filter paper of the previous colloidal gold test strip is replaced by the pH test strip, so that the pH value of the drinking water is synchronously tested, the device is more economical and practical in practical application in the mode, the operation is simple, the identification is quick, and unnecessary resource waste and time and labor are reduced.
Drawings
FIG. 1 is a schematic diagram of the main structure of a test strip for detecting pathogenic bacteria and pH value in drinking water according to the present invention;
FIG. 2 is a schematic diagram of the main body explosion resolution structure of the test strip for detecting pathogenic bacteria and pH value in drinking water according to the present invention;
FIG. 3 is a schematic diagram of a top view of a test strip for detecting pathogenic bacteria and pH in drinking water according to the present invention;
FIG. 4 is a schematic structural diagram of a test strip for detecting pathogenic bacteria and pH in drinking water according to the present invention.
In the figure: 1. a first housing; 2. a second housing; 3. a pH colorimetric card; 4. a circular groove; 5. a first slot; 6. a second slot; 7. a third slot is formed; 8. a fourth slot is formed; 9. fifthly, slotting; 10. a test strip body; 1001. a PVC base plate; 1002. a nitrocellulose membrane; 1003. a first scribe line; 1004. a second scribe line; 1005. a third scribing line; 1006. a fourth scribe line; 1007. a colloidal gold pad; 1008. a sample pad; 1009. and (4) pH test paper.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-4, the present invention provides a technical solution: the utility model provides a detect test paper strip of pathogenic bacterium in drinking water and pH value, including first casing 1, second casing 2, pH colorimetric card 3, circular recess 4, first fluting 5, second fluting 6, third fluting 7, fourth fluting 8, fifth fluting 9 and test paper strip main part 10, the block has second casing 2 on the first casing 1, and it has pH colorimetric card 3 to paste on the second casing 2, circular recess 4 has been seted up on the second casing 2, and the right side of circular recess 4 is provided with first fluting 5, second fluting 6 has been seted up on the right side of first fluting 5, and the right side of second fluting 6 is provided with third fluting 7, fourth fluting 8 has been seted up on the right side of third fluting 7, fourth fluting 8 right side is provided with fifth fluting 9, install test paper strip main part 10 between first casing 1 and the second casing 2.
The test strip body 10 comprises a PVC bottom plate 1001, a nitrocellulose membrane 1002, a first scribing line 1003, a second scribing line 1004, a third scribing line 1005, a fourth scribing line 1006, a colloidal gold pad 1007, a sample pad 1008 and a pH test paper 1009.
A nitrocellulose membrane 1002 is attached to the PVC base plate 1001, a first scribe line 1003 is arranged on the nitrocellulose membrane 1002, the position of the first scribe line 1003 corresponds to the position of the first slot 5, a second scribe line 1004 is arranged on the nitrocellulose membrane 1002, the position of the second scribe line 1004 corresponds to the position of the second slot 6, a third scribe line 1005 is arranged on the nitrocellulose membrane 1002, the position of the third scribe line 1005 corresponds to the position of the third slot 7, a fourth scribe line 1006 is arranged on the nitrocellulose membrane 1002, the position of the fourth scribe line 1006 corresponds to the position of the fourth slot 8, a colloidal gold pad 1007 is attached to the nitrocellulose membrane 1002, a sample pad 1008 is attached to the PVC base plate 1001 and the colloidal gold pad 1007, a pH test paper 1009 is attached to the PVC base plate 1001 and the nitrocellulose membrane 1002, and a fifth slot 9 is arranged right above the pH test paper.
The first open slot 5, the second open slot 6, the third open slot 7, the fourth open slot 8 and the fifth open slot 9 are all the same in opening size, and the first open slot 5, the second open slot 6, the third open slot 7, the fourth open slot 8 and the fifth open slot 9 are distributed in an equidistant array.
The test strip main body 10 has a length dimension of 10cm, and the test strip main body 10 has a width dimension of 0.5 cm.
A preparation method of a test strip for detecting pathogenic bacteria and pH value in drinking water sequentially comprises the following steps:
s1 preparation and treatment steps of colloidal gold: firstly, adding 50ml of purified water and 2ml of chloroauric acid into a clean beaker, then heating a mixture of the purified water and the chloroauric acid, heating to boil, adding 250ml of trisodium citrate solution at one time, turning off a heating device after the trisodium citrate solution is added and boiled for 10min, adding deionized water into the material after the heated material is cooled until the total mass of the material is 50g, and uniformly stirring the material and the deionized water for later use;
s2 preparation of colloidal gold label: taking 1.5ml of the material prepared in the step S1, adding 9ul of potassium carbonate solution into the material, simultaneously carrying out rapid homogenization treatment, then sequentially adding 15ug of escherichia coli antibody, 15ug of pseudomonas aeruginosa antibody and 15ug of clostridium perfringens antibody, mixing uniformly, standing for 10min, adding 15ul of confining liquid, mixing uniformly, standing for 10min, then carrying out centrifugal treatment by a centrifugal machine, removing clear liquid, re-dissolving by using colloidal gold re-dissolving solution to 0.5ml, then fully homogenizing the solution by using ultrasonic waves to obtain a colloidal gold marker, wherein the rotating speed of the centrifugal machine is 9000rpm, the centrifugal time is controlled to 7min, and the length and width of the cut glass cellulose membrane are both 5 cm;
the preparation step of the S3 colloidal gold pad 1007 is as follows: cutting the glass cellulose membrane, then placing the colloidal gold on the cut glass cellulose membrane, uniformly drying the glass cellulose membrane by adopting drying equipment, and cutting the formed colloidal gold pad 1007 into the width size of 0.5cm after drying is finished;
antibody coating step on S4 nitrocellulose membrane 1002: drawing 10 mu l of escherichia coli antibody by a pipette to match with a ruler to perform scribing at a first scribing line 1003 position on the nitrocellulose membrane 1002, then drawing 10 mu l of pseudomonas aeruginosa antibody by the pipette to match with the ruler to perform scribing at a second scribing line 1004 position on the nitrocellulose membrane 1002, then drawing 10 mu l of clostridium perfringens antibody by the pipette to match with the ruler to perform scribing at a third scribing line 1005 position on the nitrocellulose membrane 1002, and similarly drawing 10 mu l of goat anti-rabbit secondary antibody by the pipette to match with a fourth scribing line 1006 position on the nitrocellulose membrane 1002;
s5 test paper assembly preparation: the nitrocellulose membrane 1002 with the scribed lines in the S4 is attached to a PVC bottom plate 1001, the right end of a colloidal gold pad 1007 is attached to the left end of the nitrocellulose membrane 1002, a sample pad 1008 is attached to the colloidal gold pad 1007, a pH test paper 1009 is attached to the right end of the nitrocellulose membrane 1002, the assembled test paper is cut by a cutting device to be 10cm in length, the width of the test paper is 0.5cm, the test paper is placed into a first shell 1 and a second shell 2, and a pH colorimetric card 3 is attached to the second shell 2 to complete the whole assembly.
When the test strip for detecting pathogenic bacteria and pH value in drinking water is used, firstly, an assembled finished product is horizontally placed on a platform, a sample to be detected is dripped on the circular groove 4, after standing for fifteen to twenty minutes at room temperature, the color development conditions at the first open groove 5, the second open groove 6, the third open groove 7, the fourth open groove 8 and the fifth open groove 9 are observed, and the pH value of a water sample is read by comparing the pH colorimetric card 3. And those not described in detail in this specification are well within the skill of those in the art.
Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described in the foregoing embodiments, or equivalents may be substituted for elements thereof.
Claims (8)
1. The utility model provides a test paper strip of pathogenic bacterium and pH value in detection drinking water, includes first casing (1), fifth fluting (9) and test paper strip main part (10), its characterized in that: the test paper strip is characterized in that a second shell (2) is clamped on the first shell (1), a pH colorimetric card (3) is pasted on the second shell (2), a circular groove (4) is formed in the second shell (2), a first groove (5) is formed in the right side of the circular groove (4), a second groove (6) is formed in the right side of the first groove (5), a third groove (7) is formed in the right side of the second groove (6), a fourth groove (8) is formed in the right side of the third groove (7), a fifth groove (9) is formed in the right side of the fourth groove (8), and a test paper strip main body (10) is installed between the first shell (1) and the second shell (2).
2. The test strip for detecting pathogenic bacteria and pH value in drinking water according to claim 1, wherein: the test strip main body (10) comprises a PVC bottom plate (1001), a nitrocellulose membrane (1002), a first scribing line (1003), a second scribing line (1004), a third scribing line (1005), a fourth scribing line (1006), a colloidal gold pad (1007), a sample pad (1008) and a pH test paper (1009).
3. The test strip for detecting pathogenic bacteria and pH value in drinking water according to claim 2, wherein: a nitrocellulose membrane (1002) is attached to the PVC base plate (1001), a first scribing line (1003) is arranged on the nitrocellulose membrane (1002), the position of the first scribing line (1003) corresponds to the position of the first slot (5), a second scribing line (1004) is arranged on the nitrocellulose membrane (1002), the position of the second scribing line (1004) corresponds to the position of the second slot (6), a third scribing line (1005) is arranged on the nitrocellulose membrane (1002), the position of the third scribing line (1005) corresponds to the position of the third slot (7), a fourth scribing line (1006) is arranged on the nitrocellulose membrane (1002), the position of the fourth scribing line (1006) corresponds to the position of the fourth slot (8), a colloidal gold pad (1007) is attached to the nitrocellulose membrane (1002), and a sample pad (1008) is attached to the PVC base plate (1001) and the colloidal gold pad (1007), pH test paper (1009) is attached to PVC bottom plate (1001) and nitrocellulose membrane (1002), and a fifth fluting (9) has been seted up directly over pH test paper (1009).
4. The test strip for detecting pathogenic bacteria and pH value in drinking water according to claim 1, wherein: the first open groove (5), the second open groove (6), the third open groove (7), the fourth open groove (8) and the fifth open groove (9) are identical in opening size, and the first open groove (5), the second open groove (6), the third open groove (7), the fourth open groove (8) and the fifth open groove (9) are distributed in an equidistant array.
5. The test strip for detecting pathogenic bacteria and pH value in drinking water according to claim 1, wherein: the length of the test strip main body (10) is 10cm, and the width of the test strip main body (10) is 0.5 cm.
6. A preparation method of a test strip for detecting pathogenic bacteria and pH value in drinking water is characterized by sequentially comprising the following steps:
s1 preparation and treatment steps of colloidal gold: firstly, adding 50ml of purified water and 2ml of chloroauric acid into a clean beaker, then heating a mixture of the purified water and the chloroauric acid, heating to boil, adding 250ml of trisodium citrate solution at one time, turning off a heating device after the trisodium citrate solution is added and boiled for 10min, adding deionized water into the material after the heated material is cooled until the total mass of the material is 50g, and uniformly stirring the material and the deionized water for later use;
s2 preparation of colloidal gold label: taking 1.5ml of the material prepared in the step S1, adding 9ul of potassium carbonate solution into the material, simultaneously carrying out rapid homogenization treatment, then sequentially adding 15ug of escherichia coli antibody, 15ug of pseudomonas aeruginosa antibody and 15ug of clostridium perfringens antibody, mixing uniformly, standing for 10min, adding 15ul of confining liquid, mixing uniformly, standing for 10min, carrying out centrifugal treatment by a centrifuge, removing clear liquid, re-dissolving by using colloidal gold re-dissolving solution to 0.5ml, and then fully homogenizing the solution by using ultrasonic waves to obtain a colloidal gold marker;
s3 preparation of colloidal gold pad (1007): cutting the glass cellulose membrane, then placing the colloidal gold on the cut glass cellulose membrane, uniformly drying the colloidal gold by adopting drying equipment, and cutting a formed colloidal gold pad (1007) into a width size of 0.5cm after drying is finished;
antibody coating on S4 nitrocellulose membrane (1002): drawing 10 mu l of escherichia coli antibody by a pipette to match with a ruler to draw a line on a first marking (1003) position on a nitrocellulose membrane (1002), then drawing 10 mu l of pseudomonas aeruginosa antibody by the pipette to match with the ruler to draw a line on a second marking (1004) position on the nitrocellulose membrane (1002), then drawing 10 mu l of clostridium perfringens antibody by the pipette to match with the ruler to draw a line on a third marking (1005) position on the nitrocellulose membrane (1002), and drawing 10 mu l of goat anti-rabbit secondary antibody by the pipette to match with the ruler to draw a line on a fourth marking (1006) position on the nitrocellulose membrane (1002);
s5 test paper assembly preparation: the nitrocellulose membrane (1002) which is scribed in the S4 is attached to a PVC bottom plate (1001), the right end of a colloidal gold pad (1007) is attached to the left end of the nitrocellulose membrane (1002), a sample pad (1008) is attached to the colloidal gold pad (1007), pH test paper (1009) is attached to the right end of the nitrocellulose membrane (1002), the assembled test paper is cut to be 10cm in length by cutting equipment, the width of the test paper is 0.5cm, the test paper is placed into a first shell (1) and a second shell (2), and a pH colorimetric card (3) is pasted on the second shell (2) to complete the whole assembly.
7. The method for preparing the test strip for detecting pathogenic bacteria and pH value in drinking water according to claim 6, wherein the test strip comprises: the rotation speed of the centrifuge in step S2 was 9000rpm, and the centrifugation time was controlled at 7 min.
8. The method for preparing the test strip for detecting pathogenic bacteria and pH value in drinking water according to claim 6, wherein the test strip comprises: the length and width dimensions of the cellophane film after cutting in step S3 were both 5 cm.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115389760A (en) * | 2022-10-27 | 2022-11-25 | 艾康生物技术(杭州)有限公司 | Detection reagent for immunoassay test strip |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115389760A (en) * | 2022-10-27 | 2022-11-25 | 艾康生物技术(杭州)有限公司 | Detection reagent for immunoassay test strip |
| CN115389760B (en) * | 2022-10-27 | 2023-04-18 | 艾康生物技术(杭州)有限公司 | Detection reagent for immunoassay test strip |
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