CN209741187U - Cell culture device - Google Patents

Abstract

The utility model provides a cell culture device, includes the household utensils of disc the interior bottom of household utensils is equipped with first culture zone, first culture zone is equipped with the protruding ring that is used for fixed cell culture liquid and is located the inside a plurality of first micropores of protruding ring. The plurality of first micropores comprise an upper inverted circular truncated cone shape and a lower conical shape, wherein the inclination of the side surface of the inverted circular truncated cone shape to the vertical direction is 2-8 degrees, and the angle of the side surface of the conical shape to the horizontal direction is 4-10 degrees. A plurality of first micropore is the matrix and arranges, marks the number in the left side of range matrix, marks letter number in the upside of range matrix, letter number and number form two-dimensional location to every first micropore, inject the cell when every first micropore is used for cultivateing. The culture dish can be fitted with a time-delay camera, combining the advantages of culturing multiple cells together, while keeping them in place for individual monitoring and photographing.

Description

Cell culture device

Technical Field

The utility model belongs to the technical field of cell culture, especially, relate to and be a cell culture device.

Background

Embryo in vitro culture is a key step in the field of assisted reproduction, and high-quality embryos directly influence subsequent nidation or pregnancy. Embryologists wish to regularly view embryos for accurate assessment while minimizing disturbance and avoiding stress on them, which has been difficult for a long time. The time delay technique solves this problem by observing it continuously in an undisturbed culture environment. Once the shot is completed, the acquired images can be played back in a movie format, which can be remotely analyzed in a laboratory.

In conventional culture procedures, embryos must be removed from the incubator for evaluation, requiring exposure to unnecessary pressure and fluctuations. Most of the embryo culture dishes clinically used at present are ordinary flat-bottom culture dishes, and the embryos must be taken out from an incubator for observation, so that the embryo development is influenced.

SUMMERY OF THE UTILITY MODEL

To above problem, the utility model provides a device is shot in cell culture, this culture dish can cooperate the time delay, has combined the advantage of cultivateing a plurality of cells together, remains them in appropriate position so that carry out independent monitoring and shoot simultaneously, observes and assesses the cell in real time under the condition that does not disturb the knot cell.

The utility model discloses a realize through following technical scheme.

This technical scheme designs a cell culture device, household utensils including the disc the interior bottom of household utensils is equipped with first cultivation district, first cultivation district is equipped with the protruding ring that is used for fixed cell culture liquid and is located the inside a plurality of first micropores of protruding ring. The cell culture device in the technical scheme is used for culturing human embryonic cells and can also be used for culturing other cells.

Compared with the prior art, the technical scheme has the technical effects that the culture dish is internally provided with the raised circular ring, and the culture solution is added into the raised circular ring, so that the liquid drops of the culture solution are relatively fixed and are not easy to move to cause cell loss or liquid drop connection; be equipped with first micropore in the protruding ring in middle of in the culture dish, make the cell position inject in first micropore in the culture process, can cooperate shooting equipment to carry out whole realization observation or control, select the cell of optimum quality under the condition that does not disturb cell culture.

In one example of the technical solution, the plurality of first micro-holes comprise an upper inverted circular truncated cone shape and a lower conical shape, wherein the side surface of the inverted circular truncated cone shape has an inclination of 2-8 degrees with the vertical direction, and the angle of the side surface of the conical shape with the horizontal direction is 4-10 degrees. The structure of the first micropore enables the middle of the bottom of the first micropore to slightly sink, so that cells are always positioned in the middle of the first micropore in the first micropore, and real-time observation and shooting of the cells are facilitated.

In an example of this technical scheme, further, a plurality of first micropore is arranged as the matrix, marks the number in the left side of matrix, marks the letter number in the upside of matrix, letter number and number form two-dimentional location to every first micropore, and every first micropore is used for injecing the cell when cultivateing. The upper side and the left side of the micropore matrix are provided with marks for marking the position of each cell, and corresponding cells can be quickly found.

In an example of this technical solution, further, the distance between the first micropores is 0.22-0.28mm, and the matrix is located at the center of the convex circular ring.

In an example of the technical solution, further, the diameter of the upper circle of the inverted truncated cone shape of the first micro-hole is 0.15-0.45mm, the diameter of the lower circle is 0.25-0.31mm, and the depth of the first micro-hole is 0.13-0.19 mm.

In one example of this solution, the disc-shaped vessel is provided with a wave-shaped hand grip at its periphery. The culture dish periphery middle part position is equipped with the handheld portion of round wave, is favorable to the culture dish to hand, is difficult to the landing.

In one example of the technical scheme, further, the inner diameter of the convex circular ring is 5-9mm, the outer diameter is 6-10mm, and the height of the convex is 0.8-1.2 mm.

In an example of the technical solution, further, a second culture zone is further provided at the inner bottom of the vessel, a second micropore is provided in the second culture zone, the second micropore is a pointed hole, and the inner side wall of the second micropore is an outwardly concave arc surface.

In an example of the technical solution, further, a third culture zone is further arranged at the inner bottom of the vessel, third micro-holes are arranged in the third culture zone, the bottom of each third micro-hole is flat, the inner diameter of the mouth part is larger than that of the bottom part, and the inner side wall of each third micro-hole is an outwardly concave arc surface.

In one example of this embodiment, the number of the first culture region, the second culture region or the third culture region is one or two or more.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required to be used in the description of the embodiments of the present invention will be briefly introduced below, and the drawings in the following description are only directed to some embodiments, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without inventive labor.

Fig. 1 is a perspective view of a culture dish according to the first embodiment of the present invention.

Fig. 2 shows a front view of a culture dish according to the first embodiment of the present application.

Fig. 3 shows a cross-sectional view of the culture dish in fig. 2 in the direction a-a.

FIG. 4 shows an enlarged view of a first culture zone in the first embodiment of the present application.

Fig. 5 shows a cross-sectional view of a first micro-cell according to a first embodiment of the present application.

Fig. 6 shows a front view of a culture dish according to example two of the present application.

fig. 7 shows a cross-sectional view of a second micro-cell of example two of the present application.

Fig. 8 shows a cross-sectional view of a third micro-cell of example two of the present application.

Detailed Description

Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary and intended to be used for explaining the present invention, and should not be construed as limiting the present invention.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.

In the present invention, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

In the present disclosure, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may comprise direct contact between the first and second features, or may comprise contact between the first and second features not directly. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.

In addition, the technical solutions in the embodiments may be combined with each other, but it must be based on the realization of those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should be considered to be absent, and is not within the scope of the present disclosure.

In the following description, suffixes such as "module", "component", "assembly", or "unit" are used only for the convenience of description of the present invention, and do not have a specific meaning per se. And thus may be used mixedly.

The present invention will be described in further detail with reference to the accompanying drawings.

Example one

Referring to fig. 1 to 5, the present embodiment is a human embryo in vitro culture dish for assisting the culture of embryo in reproductive laboratory, comprising a disc-shaped dish 10, wherein the inner bottom of the dish is provided with a first culture region 11 and a cleaning region 12, the first culture region is provided with a raised circular ring 13, the raised circular ring is used for fixing the culture solution of embryo, wherein the raised circular ring is provided with 25 first micropores 111, the first micropores are arranged in a 5 x 5 matrix, the embryo is placed in the first micropores, and the first micropores are used for fixing the embryo during culture. The washing zone is also surrounded by a raised ring, and if necessary, the culture dish may also comprise an upper cover which cooperates with the dish-shaped vessel.

The disc-shaped vessel has an inner diameter of 35mm and a height of 10mm, and is provided at the outer periphery thereof with a wavy hand-held portion 14. The inner diameter of the convex circular ring is 7mm, the outer diameter of the convex circular ring is 8mm, and the height of the convex circular ring is 1 mm.

The matrix formed by the first micropores is positioned in the center of the convex ring, the distance between the adjacent first micropores is 0.25mm, the left side of the matrix is provided with a number 112 for each row of the matrix, and the number is '1, 2, 3, 4, 5'; the letter number 113 is arranged on each column of the matrix on the upper side of the matrix, and the letter numbers are 'A, B, C, D and E'; based on the number and letter numbers, each first microwell can be positioned in two dimensions, e.g., the first microwell in the upper left corner can be positioned with a1 and the first microwell in the lower right corner can be positioned with D5.

The first micro-hole is of an inner concave hole structure and mainly comprises an upper inverted circular truncated cone shape 1111 and a lower conical shape 1112, wherein the inclination of the side surface of the inverted circular truncated cone shape to the vertical direction is 5 degrees, and the angle of the side surface of the conical shape to the horizontal direction is 7 degrees. The diameter of the upper circle of the first micropore is 0.3mm, the diameter of the lower circle of the first micropore is 0.28mm, and the depth of the first micropore is 0.16 mm.

Example two

Referring to fig. 6 to 8, this embodiment is a human embryo in vitro culture dish, which is used for assisting the culture of a reproductive laboratory embryo, and is different from the first embodiment in that:

In addition to the first culture zone 11, a second culture zone 15, a third culture zone 16 and two spare zones are provided at the inner bottom of the vessel, wherein the two spare zones can be used as a washing zone 12 for washing embryos or a temporary placement zone 17 for temporarily placing embryos.

And a second micropore 151 is arranged in the second culture area, is a pointed hole, and has an inner side wall which is an outwardly concave cambered surface. The first micro-hole is an inverted sharp bottom and is a roughly conical hole which is similar to a sharp leaf shape.

And a third micropore 161 is arranged in the third culture area, the bottom of the third micropore is flat, the inner diameter of the opening part is larger than that of the bottom, and the inner side wall of the third micropore is an outwardly concave cambered surface.

In the description of the specification, reference to the terms "one embodiment," "some embodiments," "one example," "a specific example," or "some examples" or the like means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

Claims (10)

1. The utility model provides a cell culture device, its characterized in that, includes the household utensils of disc the interior bottom of household utensils is equipped with first culture zone, first culture zone is equipped with the protruding ring that is used for fixed cell culture liquid and is located the inside a plurality of first micropores of protruding ring.

2. The cell culture apparatus of claim 1, wherein the plurality of first micro-wells comprise an upper reverse truncated cone shape and a lower conical shape, wherein the side of the reverse truncated cone shape has an inclination of 2 to 8 degrees with respect to the vertical direction, and the side of the conical shape has an angle of 4 to 10 degrees with respect to the horizontal direction.

3. The cell culture apparatus of claim 2, wherein the first plurality of wells are arranged in a matrix, and wherein the matrix is provided with a number on the left or upper side of the matrix, and wherein the matrix is provided with a letter number on the upper or left side of the matrix, wherein the letter number and the number are positioned in two dimensions for each first well, and wherein each first well defines a cell during culture.

4. The cell culture apparatus of claim 3, wherein the distance between the first micro-wells is 0.22-0.28mm, and the matrix is located at the center of the convex circle.

5. The cell culture apparatus of claim 3, wherein the first micro-holes have an upper circle of the inverted truncated cone shape having a diameter of 0.15-0.45mm, a lower circle of the inverted cone shape having a diameter of 0.25-0.31mm, and a depth of 0.13-0.19 mm.

6. The cell culture apparatus of any one of claims 1 to 5, wherein the disc-shaped vessel is provided with a wave-shaped hand grip at its outer periphery.

7. The cell culture apparatus according to claim 6, wherein the inner diameter of the convex circular ring is 5 to 9mm, the outer diameter is 6 to 10mm, and the height of the protrusion is 0.8 to 1.2 mm.

8. The cell culture device according to claim 6, wherein a second culture zone is further provided at the inner bottom of the vessel, second micro-holes are provided in the second culture zone, the second micro-holes are tip-like holes, and inner side walls thereof are outwardly concave cambered surfaces.

9. The cell culture device according to claim 8, wherein a third culture zone is further provided at the inner bottom of the vessel, third micro-holes are provided in the third culture zone, the bottom of the third micro-holes is flat, the inner diameter of the mouth part is larger than that of the bottom part, and the inner side wall of the third micro-holes is an outwardly concave arc surface.

10. The cell culture apparatus of claim 9, wherein the number of the first, second or third culture zones is one or more than two.