CN209722181U - Minim DNA sample blending device for gel electrophoresis - Google Patents
Minim DNA sample blending device for gel electrophoresis Download PDFInfo
- Publication number
- CN209722181U CN209722181U CN201920425403.9U CN201920425403U CN209722181U CN 209722181 U CN209722181 U CN 209722181U CN 201920425403 U CN201920425403 U CN 201920425403U CN 209722181 U CN209722181 U CN 209722181U
- Authority
- CN
- China
- Prior art keywords
- conical
- fixed
- rotating body
- gel electrophoresis
- fixed block
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model belongs to technical field of biological, more particularly to a kind of minim DNA sample blending device for gel electrophoresis, including pedestal, susceptor surface is vertically and fixedly provided with vertical support rod, support rod top, which is respectively perpendicular, is fixed with first crossbar, second crossbar, first crossbar end is fixed with cylindrical fixed block, second crossbar end is fixed with conical vessel, fixed block is equipped with longitudinal through its internal centre bore, vertical bar is equipped in centre bore, rotation knob is equipped at the top of vertical bar, it is fixed at vertical bar bottom and the center of conical rotating body upper surface, conical rotating body is located inside conical vessel, and there is stirring gap between conical rotating body outer surface and conical vessel inner surface.Two kinds of liquid of the utility model stir in the stirring gap between conical rotating body outer surface and conical vessel inner surface, and be suitble to a small amount of liquid stirs and evenly mixs operation.
Description
Technical field
The utility model belongs to technical field of biological, and in particular to a kind of minim DNA sample for gel electrophoresis
Evenly mixing device.
Background technique
In molecule experiments the agarose gel electrophoresis of Plasmid DNA " separate ", need to by Plasmid DNA test sample and
Loading Buffer (sample-loading buffer) is added in the well of gel again after mixing well.In laboratory, it is usually prepared for
Gelatin band is pasted on laboratory table, is respectively moved to two kinds of liquid on adhesive tape with liquid-transfering gun, is leaned on liquid-transfering gun " inspiration blowout "
Process repeatedly makes two kinds of liquid blendings, this lack of standardization and adhesive tape of operation, which is adhered on laboratory table, easily causes pollution, simultaneously as
The dosage of two kinds of liquid is less when experiment, is unsuitable for stirring and evenly mixing in existing mixing plant.
Utility model content
Against the above technical problems, the utility model aim is to provide a kind of minim DNA sample for gel electrophoresis
Evenly mixing device.
Minim DNA sample blending device described in the utility model for gel electrophoresis, including pedestal, susceptor surface are hung down
It is directly fixed with vertical support rod, support rod top, which is respectively perpendicular, is fixed with first crossbar, second crossbar, and first crossbar end is solid
Surely there is cylindrical fixed block, second crossbar end is fixed with conical vessel, and fixed block is equipped with longitudinal through its internal center
Hole, centre bore is interior to be equipped with vertical bar, is fixed with the first limit film, the vertical bar of fixed block lower surface on the vertical bar at fixed block upper surface
On be fixed with the second limit film, the first limit film is close to fixed block upper surface, and the second limit film is close to fixed block lower surface, vertical bar
Top is equipped with rotation knob, fixes at vertical bar bottom and the center of conical rotating body upper surface, conical rotating body is located at conical vessel
Inside, and there is stirring gap between conical rotating body outer surface and conical vessel inner surface, conical rotating body outer surface is set
If there is dry elasticity burr, conical vessel bottom is connect with grass tube, and conical vessel and grass tube junction are living equipped with glass
Plug.
Conical rotating body upper surface raises upward to form tapered slope, is conducive to pour into the liquid on conical rotating body
It is slid into stirring gap under the effect of gravity.
Elastic burr is rubber billet.
Elastic burr is mixed and disorderly or be uniformly distributed in conical rotating body outer surface.
Conical vessel is glass material.
Conical vessel inner surface coats one layer of polytetrafluorethylecoatings coatings.
The utility model compared with prior art, has the advantages that.
(1) the Plasmid DNA test sample and Loading Buffer (sample-loading buffer) stirring that the utility model solves are mixed
Even technical problem；
(2) stirring gap of the two kinds of liquid of the utility model between conical rotating body outer surface and conical vessel inner surface
Interior stirring, be suitble to a small amount of liquid stirs and evenly mixs operation.
Detailed description of the invention
Fig. 1, an embodiment of the present invention structural schematic diagram；
It Fig. 2, is conical rotating body perspective view in Fig. 1；
It Fig. 3, is partial enlarged view in Fig. 1 centre circle.
In figure: 1, pedestal 2, grass tube 3, glass piston 4, elastic burr 5, conical vessel 6, conical rotating body
7, tapered slope 8, vertical bar 9, the second limit film 10, fixed block 11, the first limit film 12, rotation knob 13, support rod
14, first crossbar 15, second crossbar 16, centre bore.
Specific embodiment
The utility model is described further below with reference to embodiment and Figure of description.
As shown in Figure 1-3, the minim DNA sample blending device described in the utility model for gel electrophoresis, including bottom
Seat 1,1 surface of pedestal are vertically and fixedly provided with vertical support rod 13, and 13 top of support rod, which is respectively perpendicular, is fixed with first crossbar 14, the
Two cross bars 15,14 end of first crossbar are fixed with cylindrical fixed block 10, and 15 end of second crossbar is fixed with conical vessel 5,
Fixed block 10 is equipped with longitudinal centre bore 16 through its inside, and vertical bar 8 is equipped in centre bore 16, perpendicular at 10 upper surface of fixed block
It is fixed with the first limit film 11 on bar 8, the second limit film 9, the first limit film 11 are fixed on the vertical bar 8 of 10 lower surface of fixed block
It is close to 10 upper surface of fixed block, the second limit film 9 is close to 10 lower surface of fixed block, rotation knob 12, vertical bar 8 are equipped at the top of vertical bar 8
It is fixed at bottom and the center of 6 upper surface of conical rotating body, conical rotating body 6 is located inside conical vessel 5, and taper rotates
There is stirring gap, if 6 outer surface of conical rotating body is equipped with dry elasticity burr between 5 inner surface of 6 outer surface of body and conical vessel
4,5 bottom of conical vessel is connect with grass tube 2, and conical vessel 5 and 2 junction of grass tube are equipped with glass piston 3.
6 upper surface of conical rotating body raises upward to form tapered slope 7.
Elastic burr 4 is rubber billet.
Elastic burr 4 is distributed in a jumble in 6 outer surface of conical rotating body.
Conical vessel 5 is glass material.
5 inner surface of conical vessel coats one layer of polytetrafluorethylecoatings coatings.
The utility model is before use, first close glass piston 3, by two kinds of liquid importing 6 outer surfaces of conical rotating body
Between 5 inner surface of conical vessel in interior stirring gap, rotation knob 12 is back and forth twisted using thumb and index finger, drives taper rotation
Quickly rotation, elastic burr 4 fast ram, two kinds of liquid of 6 outer surface of conical rotating body mix well two kinds of liquid to swivel 6,
After mixing, to open glass piston 3 and release liquid, the utility model is suitable for the operation that a small amount of liquid agitation mixes,
It is simple and convenient.
Claims (6)
1. a kind of minim DNA sample blending device for gel electrophoresis, including pedestal (1), pedestal (1) surface are vertically and fixedly provided with
Vertical support rod (13), it is characterised in that: support rod (13) top, which is respectively perpendicular, is fixed with first crossbar (14), second crossbar
(15), first crossbar (14) end is fixed with cylindrical fixed block (10), and second crossbar (15) end is fixed with conical vessel
(5), fixed block (10) is equipped with longitudinal through its internal centre bore (16), and centre bore (16) is interior to be equipped with vertical bar (8), fixed block
(10) it is fixed with the first limit film (11) on the vertical bar at upper surface (8), is fixed on the vertical bar (8) of fixed block (10) lower surface
Second limit film (9), the first limit film (11) are close to fixed block (10) upper surface, and the second limit film (9) is close to fixed block (10)
Lower surface, vertical bar (8) top are equipped with rotation knob (12), consolidate at vertical bar (8) bottom and the center of conical rotating body (6) upper surface
Fixed, conical rotating body (6) is located at conical vessel (5) inside, and conical rotating body (6) outer surface and conical vessel (5) interior table
There is stirring gap, conical rotating body (6) is if outer surface is equipped with dry elasticity burr (4), conical vessel (5) bottom and glass between face
Glass conduit (2) connection, conical vessel (5) and grass tube (2) junction are equipped with glass piston (3).
2. the minim DNA sample blending device according to claim 1 for gel electrophoresis, it is characterised in that: taper rotation
Swivel (6) upper surface raises upward to form tapered slope (7).
3. the minim DNA sample blending device according to claim 1 for gel electrophoresis, it is characterised in that: elastic hair
Piercing (4) is rubber billet.
4. the minim DNA sample blending device according to claim 1 or 3 for gel electrophoresis, it is characterised in that: elasticity
Burr (4) is mixed and disorderly or be uniformly distributed in conical rotating body (6) outer surface.
5. the minim DNA sample blending device according to claim 1 for gel electrophoresis, it is characterised in that: taper is held
Device (5) is glass material.
6. the minim DNA sample blending device according to claim 1 for gel electrophoresis, it is characterised in that: taper is held
Device (5) inner surface coats one layer of polytetrafluorethylecoatings coatings.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920425403.9U CN209722181U (en) | 2019-03-29 | 2019-03-29 | Minim DNA sample blending device for gel electrophoresis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920425403.9U CN209722181U (en) | 2019-03-29 | 2019-03-29 | Minim DNA sample blending device for gel electrophoresis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209722181U true CN209722181U (en) | 2019-12-03 |
Family
ID=68689584
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920425403.9U Expired - Fee Related CN209722181U (en) | 2019-03-29 | 2019-03-29 | Minim DNA sample blending device for gel electrophoresis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209722181U (en) |
-
2019
- 2019-03-29 CN CN201920425403.9U patent/CN209722181U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20191203 Termination date: 20210329 |