CN109632435B - Dyeing pen for dyeing biological tissue slices - Google Patents
Dyeing pen for dyeing biological tissue slices Download PDFInfo
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- CN109632435B CN109632435B CN201910059112.7A CN201910059112A CN109632435B CN 109632435 B CN109632435 B CN 109632435B CN 201910059112 A CN201910059112 A CN 201910059112A CN 109632435 B CN109632435 B CN 109632435B
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- liquid storage
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- 238000004043 dyeing Methods 0.000 title claims abstract description 69
- 239000007788 liquid Substances 0.000 claims abstract description 177
- 238000010186 staining Methods 0.000 claims abstract description 28
- 239000012192 staining solution Substances 0.000 claims abstract description 3
- 230000007797 corrosion Effects 0.000 claims description 3
- 238000005260 corrosion Methods 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 2
- 239000011550 stock solution Substances 0.000 claims 3
- 230000000694 effects Effects 0.000 abstract description 3
- 239000002699 waste material Substances 0.000 description 7
- 239000003086 colorant Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 2
- 239000013049 sediment Substances 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002550 fecal effect Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000002485 urinary effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Pens And Brushes (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention belongs to the field of biological experiment devices, and particularly relates to a staining pen for staining biological tissue slices. The dyeing pen consists of a pen holder, a pen point, a liquid storage tube, a pen cover and a pressing part. The pen holder and the pen point are integrally formed, the liquid storage tube is embedded in the pen holder, and the pen cover is screwed; after the button is pressed, the staining solution flows out from the liquid storage tube to the pen point, and the biological tissue slice can be stained. The pressing time of the button is controlled to control the amount of the dyeing liquid, so that the problem of poor dyeing effect caused by uneven dyeing is avoided. The dyeing pen provided by the invention has the advantages of convenience and rapidness in operation, safety in use, uniformity in dyeing, good dyeing effect and the like.
Description
Technical Field
The invention belongs to the field of biological experiment devices, and particularly relates to a staining pen for staining biological tissue slices.
Background
In the fields of life science and medicine, animal and plant tissue morphological structures are observed, histopathological examination is carried out, and blood smear, urinary sediment and fecal sediment detection are not separated from tissue staining. At present, the dyeing reagent purchased in the market mainly adopts a bottle, an experimenter utilizes a dropper, a pipette or a pipetting gun to adsorb during dyeing, the dyeing liquid is added into a tissue slice, but in the process, the consumption of the dyeing liquid is difficult to control, the dyeing liquid can be inclined and spilled due to carelessness, the dye color is deeper, the waste is caused, the experiment cost is increased, and the dyeing liquid is difficult to clean once being stained, so that the damage to a human body is large. If the liquid-transfering gun with small measuring range is used for quantitatively sucking, repeated operation is time-consuming and labor-consuming, the same batch of chips can be caused, the dyeing time is inconsistent, and the ideal dyeing effect is difficult to achieve.
Aiming at the technical problems, the invention discloses a dyeing pen for dyeing biological tissue slices, which can effectively control the dosage of dyeing liquid and the dyeing uniformity, and effectively solve the technical problems.
Disclosure of Invention
The invention aims to provide a staining pen for staining biological tissue slices, and discloses a using method of the staining pen in detail. The specific technical scheme is as follows:
The dyeing pen for dyeing the biological tissue slices comprises a pen holder 1, a pen head 2, a liquid storage tube 3 and a pen cover 4, wherein the pen head 2 and the pen cover 4 are respectively arranged at two ends of the pen holder 1, the liquid storage tube 3 is arranged in the pen holder 1, one end, close to the pen head 2, of the pen holder 1 is provided with a pressing hole 103 for fixing a button 501, one end, close to the pen head 2, of the liquid storage tube 3 is provided with a liquid outlet 303, the dyeing pen for dyeing the biological tissue slices further comprises a pressing part 5, the pressing part 5 comprises a button 501, a baffle 502, a push rod 503 and an elastic component 504, the button 501 is fixed on the pressing hole 103, and the baffle 502 is connected to the liquid outlet 303 through an elastic component 504 to completely cover the liquid outlet 303; the push rod 503 is Y-shaped, the front end is connected with the baffle 502, the rear end is connected with the button 501, when the button 501 is pressed, the push rod 503 is pressed to transversely push the baffle 502, the liquid outlet 303 is exposed, when the button 501 is released after dyeing is finished, the elastic component 504 can enable the baffle 502 to recover to the original position, the liquid outlet 303 is covered again, and the dyeing liquid is sealed in the liquid storage tube.
The pen holder 1 is further provided with a pen holder body 101 and clamping plates 102, the pen holder body 101 is hollow and cylindrical, the clamping plates 102 are arranged on the inner wall surface of the pen holder body 101, the number of the clamping plates 102 is more than or equal to 1, and the clamping plates are used for fixing the liquid storage tube 3 and preventing the liquid storage tube 3 from shaking left and right to influence use.
The pen point 2 is funnel-shaped, is arranged at one end of the pen holder 1, is integrally formed with the pen holder 1, and consists of a liquid collecting tank 201 and a liquid outlet 202, wherein the liquid collecting tank 201 is funnel-shaped, so that dyeing liquid flowing out of the liquid outlet 303 is conveniently collected, and then flows out along the liquid outlet 202, the liquid outlet 202 is hollow cylindrical, and the liquid outlet 202 is arranged at the tail end of the liquid collecting tank 201 and is integrally formed with the liquid collecting tank 201.
The liquid storage tube 3 comprises a liquid storage tube body 301, a liquid outlet tube 302 and a liquid outlet 303, wherein the liquid storage tube body 301 is hollow cylindrical, one end of the liquid storage tube body 301 is connected with the liquid outlet tube 302, the liquid outlet 303 is arranged at the tail end of the liquid outlet tube 302, the diameter of the liquid outlet tube 302 is smaller than that of the liquid storage tube body 301, the outflow speed of dyeing liquid is controlled, and the liquid storage tube body 301, the liquid outlet tube 302 and the liquid outlet 303 are integrally formed.
The rear part of the liquid storage tube body 301 is provided with a valve, external air enters the liquid storage tube 3 through the valve, the atmospheric pressure inside and outside the liquid storage tube 3 is balanced, but the dyeing liquid cannot flow out, and the arrangement of the valve effectively prevents the dyeing liquid from flowing out from the rear end of the liquid storage tube 3 to cause pollution or waste.
The pen cap 4 is arranged at one end of the pen holder body 101 far away from the pen head 2, and is composed of a pen cap body 401, a vent hole 402 and a limiting groove 404, wherein the limiting groove 404 is arranged on the pen cap body 401, the limiting groove 404 is in a ring shape, the limiting groove 404 is buckled at the tail end of the pen holder body 101, the pen cap body 401 is fixed on the pen holder body 101 through the limiting groove 404, the vent hole 402 is arranged at the center of the pen cap body 401 and penetrates through the pen cap body 401, the vent hole 402 can balance the atmospheric pressure inside and outside the liquid storage tube 3, but liquid cannot flow out of the liquid storage tube, so that pollution and waste are caused.
The liquid outlet 303 is an inclined section of the liquid outlet pipe 302, is elliptical, has an inclined included angle of 30-60 degrees, the elastic component 504 is arranged on the upper part of the inclined section, and the push rod 503 is arranged at two ends of the baffle 502 perpendicular to the liquid outlet pipe 302, so that pollution to the push rod 503 after the dyeing liquid flows out is effectively prevented.
Preferably, the pen cap 4 is further provided with a limit groove 403, the limit groove 403 is arranged on an inner ring of the limit groove 404, the outer diameter of the limit groove 403 is smaller than the inner diameter of the limit groove 404, the limit groove 403 is tightly sleeved with the bottom end of the liquid storage tube body 301, and the dye liquid is prevented from being poured due to the inclination of the dye pen, so that pollution and waste are caused.
Preferably, the dyeing pen is further provided with a pen cap 6, the color of the pen cap 6 is the same as that of the dyeing liquid contained in the liquid storage tube 3, damage to the pen head 2 and pollution of the dyeing liquid can be prevented, different colors represent different types of dyeing pens, and an experimenter can conveniently distinguish different dyeing liquids in operation.
Preferably, the liquid storage tube 3 is made of corrosion-resistant materials, so that the corrosion of the liquid storage tube caused by the dyeing liquid is prevented, and the service life of the dyeing pen is prolonged.
The beneficial effects of the invention are as follows: ① The liquid storage tube of the dyeing pen has large capacity, and compared with a conventional liquid-transfering gun, the liquid-transfering gun reduces the times of suction, saves time and labor; ② The experimenter can determine the time for pressing the button according to the using amount of the dyeing liquid, and can dye at one time, so that the uneven dyeing caused by inconsistent operation time is reduced; ③ Compared with the conventional dropper, can effectively prevent the staining solution from toppling over, the waste of the dyeing liquid is reduced, and the pollution of the dyeing liquid is reduced; ④ The pen cap and the pen cap of the dyeing pen can be made into different colors according to the colors of the dyeing liquid, so that the experimenters can conveniently distinguish different dyeing liquids during operation; ⑤ The structural design of the dyeing pen accords with the human body mechanics law, and the operation is very laborsaving.
Drawings
FIG. 1 is a block diagram of a staining pen for staining biological tissue sections
1, A penholder; 101, a pen holder body; 102, clamping plates; 103, pressing the hole; 2, a pen point; 201, a liquid collecting tank; 202, a liquid outlet; 3, a liquid storage tube; 301, a liquid storage tube body; 302, a liquid outlet pipe; 303, a liquid outlet; 4, a pen cap; 401 a pen cap body; 402, vent holes; 403, a limit groove; 404, a limit groove; 5, an operation part; 501, operating a button; 502, a baffle; 503, pushing rod; 504, an elastic component; 6, a pen cap.
Detailed Description
The following embodiments are further described in detail to illustrate the present invention, but the scope of the present invention is not limited to the following embodiments. Example 1A staining pen for staining biological tissue sections
The dyeing pen for dyeing biological tissue slices comprises a pen holder 1, a pen head 2, a liquid storage tube 3 and a pen cover 4, wherein the pen head 2 and the pen cover 4 are respectively arranged at two ends of the pen holder 1, the liquid storage tube 3 is arranged in the pen holder 1, one end of the pen holder 1, which is close to the pen head 2, is provided with a pressing hole 103 for fixing a button 501, one end of the liquid storage tube 3, which is close to the pen head 2, is provided with a liquid outlet 303, the dyeing pen for dyeing biological tissue slices further comprises a pressing component 5, the pressing component 5 comprises a button 501, a baffle 502, a push rod 503 and an elastic component 504, the button 501 is fixed on the pressing hole 103, the baffle 502 is connected to the liquid outlet 303 through an elastic component 504 to completely cover the liquid outlet 303; the push rod 503 be "Y" type, the front end is connected with baffle 502, the rear end is connected with button 501, when pressing the button 501, presses the push rod 503 and transversely promotes baffle 502, makes liquid outlet 303 expose, and the dyeing liquid flows from liquid outlet 303, and when dyeing end unclamp button 501, elastic component 504 can make baffle 502 resume the normal position, cover liquid outlet 303 again, seal the dyeing liquid in the liquid storage tube. The pen holder 1 is further provided with a pen holder body 101 and clamping plates 102, the pen holder body 101 is hollow and cylindrical, the clamping plates 102 are arranged on the inner wall surface of the pen holder body 101, the number of the clamping plates 102 is more than or equal to 1, and the clamping plates are used for fixing the liquid storage tube 3 and preventing the liquid storage tube 3 from shaking left and right to influence use. The pen point 2 is funnel-shaped, is arranged at one end of the pen holder 1, is integrally formed with the pen holder 1, and consists of a liquid collecting tank 201 and a liquid outlet 202, wherein the liquid collecting tank 201 is funnel-shaped, so that dyeing liquid flowing out of the liquid outlet 303 is conveniently collected, and then flows out along the liquid outlet 202, the liquid outlet 202 is hollow cylindrical, and the liquid outlet 202 is arranged at the tail end of the liquid collecting tank 201 and is integrally formed with the liquid collecting tank 201. The liquid storage tube 3 comprises a liquid storage tube body 301, a liquid outlet tube 302 and a liquid outlet 303, wherein the liquid storage tube body 301 is hollow cylindrical, one end of the liquid storage tube body 301 is connected with the liquid outlet tube 302, the liquid outlet 303 is arranged at the tail end of the liquid outlet tube 302, the diameter of the liquid outlet tube 302 is smaller than that of the liquid storage tube body 301, the outflow speed of dyeing liquid is controlled, and the liquid storage tube body 301, the liquid outlet tube 302 and the liquid outlet 303 are integrally formed. The rear part of the liquid storage tube body 301 is provided with a valve, external air enters the liquid storage tube 3 through the valve, the atmospheric pressure inside and outside the liquid storage tube 3 is balanced, but the dyeing liquid cannot flow out, and the arrangement of the valve effectively prevents the dyeing liquid from flowing out from the rear end of the liquid storage tube 3 to cause pollution or waste. The pen cap 4 is arranged at one end of the pen holder body 101 far away from the pen head 2, and is composed of a pen cap body 401, a vent hole 402 and a limiting groove 404, wherein the limiting groove 404 is arranged on the pen cap body 401, the limiting groove 404 is in a ring shape, the limiting groove 404 is buckled at the tail end of the pen holder body 101, the pen cap body 401 is fixed on the pen holder body 101 through the limiting groove 404, the vent hole 402 is arranged at the center of the pen cap body 401 and penetrates through the pen cap body 401, the vent hole 402 can balance the atmospheric pressure inside and outside the liquid storage tube 3, but liquid cannot flow out of the liquid storage tube, so that pollution and waste are caused. The liquid outlet 303 is an inclined section of the liquid outlet pipe 302, is elliptical, has an inclined included angle of 30-60 degrees, the elastic component 504 is arranged on the upper part of the inclined section, and the push rod 503 is arranged at two ends of the baffle 502 perpendicular to the liquid outlet pipe 302, so that pollution to the push rod 503 after the dyeing liquid flows out is effectively prevented.
Example 2 use of a staining pen for staining biological tissue sections
Before a biological tissue slice staining experiment, a pen cover 4 of a staining pen is opened, staining liquid for staining is filled into an inner liquid storage tube 3, the pen cover 4 is tightly covered, when a button 501 is pressed, a push rod 503 is pressed to transversely push a baffle 502, a liquid outlet 303 is exposed, the staining liquid flows out of the liquid outlet 303, when the button 501 is released after staining is finished, an elastic component 504 enables the baffle 502 to restore to the original position, the liquid outlet 303 is covered again, the staining liquid is sealed in the liquid storage tube, staining is stopped, a pen cap 6 is covered, a pen head 2 is protected, and staining liquid pollution is prevented. The pen cap 6 can be made into different colors according to the color of the dyeing liquid, so that an experimenter can quickly distinguish the dyeing liquid during dyeing.
Claims (3)
1. A dye pen for biological tissue section dyeing, dye pen constitute by pen-holder (1), nib (2), stock solution pipe (3) and pen lid (4), nib (2) and pen lid (4) set up respectively in the both ends of pen-holder (1), stock solution pipe (3) set up inside pen-holder (1), pen-holder (1) be close to the one end of nib (2) and be provided with press hole (103), the one end that stock solution pipe (3) are close to nib (2) is provided with second liquid outlet (303), its characterized in that:
the dyeing pen for dyeing the biological tissue slice further comprises a pressing component (5), wherein the pressing component (5) consists of a button (501), a baffle plate (502), a push rod (503) and an elastic component (504), the button (501) is fixed on the pressing hole (103), and the baffle plate (502) is connected to the second liquid outlet (303) through the elastic component (504) to completely cover the second liquid outlet (303); the push rod (503) is Y-shaped, the front end of the push rod is connected with the baffle (502), and the rear end of the push rod is connected with the button (501);
The pen holder (1) is further provided with a pen holder body (101) and clamping plates (102), the pen holder body (101) is hollow and cylindrical, the clamping plates (102) are arranged on the inner wall surface of the pen holder body (101), and the number of the clamping plates (102) is more than or equal to 1;
The pen point (2) is funnel-shaped, is arranged at one end of the pen holder (1), is integrally formed with the pen holder (1), and consists of a liquid collecting tank (201) and a first liquid outlet (202), wherein the liquid collecting tank (201) is funnel-shaped, the first liquid outlet (202) is hollow cylindrical, and the first liquid outlet (202) is arranged at the tail end of the liquid collecting tank (201) and is integrally formed with the liquid collecting tank (201);
The liquid storage pipe (3) consists of a liquid storage pipe body (301), a liquid outlet pipe (302) and a second liquid outlet (303), wherein the liquid storage pipe body (301) is hollow and cylindrical, one end of the liquid storage pipe body is connected with the liquid outlet pipe (302), the second liquid outlet (303) is arranged at the tail end of the liquid outlet pipe (302), the diameter of the liquid outlet pipe (302) is smaller than that of the liquid storage pipe body (301), and the liquid storage pipe body (301), the liquid outlet pipe (302) and the second liquid outlet (303) are integrally formed;
The rear part of the liquid storage pipe body (301) is provided with a valve, external air enters the liquid storage pipe (3) through the valve, and the atmospheric pressure inside and outside the liquid storage pipe (3) is balanced, but the dyeing liquid cannot flow out;
The pen cover (4) is arranged at one end, far away from the pen point (2), of the pen cover body (101) and consists of a pen cover body (401), an air vent (402) and a second limit groove (404), the second limit groove (404) is arranged on the pen cover body (401), the second limit groove (404) is in a ring shape, the second limit groove (404) is buckled at the tail end of the pen cover body (101), the pen cover body (401) is fixed on the pen cover body (101) through the second limit groove (404), the air vent (402) is arranged at the center of the pen cover body (401) and penetrates through the pen cover body (401), and the air vent (402) can balance the atmospheric pressure inside and outside the liquid storage tube (3) but liquid cannot flow out of the air vent;
The second liquid outlet (303) is an inclined section of the liquid outlet pipe (302), is elliptical, has an inclined plane included angle of 30-60 degrees, the elastic component (504) is arranged at the upper part of the inclined section, and the push rod (503) is arranged at two ends of the baffle (502) perpendicular to the liquid outlet pipe (302);
The pen cap (4) is further provided with a first limit groove (403), the first limit groove (403) is arranged on the inner ring of the second limit groove (404), the outer diameter of the first limit groove (403) is smaller than the inner diameter of the second limit groove (404), and the first limit groove (403) is tightly sleeved with the bottom end of the liquid storage tube body (301).
2. A staining pen for use in staining biological tissue sections according to claim 1 wherein the staining pen is further provided with a cap (6), the cap (6) being of the same colour as the staining solution contained in the reservoir (3).
3. A staining pen for staining biological tissue sections according to claim 1 wherein the reservoir (3) is made of a corrosion resistant material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910059112.7A CN109632435B (en) | 2019-01-22 | 2019-01-22 | Dyeing pen for dyeing biological tissue slices |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910059112.7A CN109632435B (en) | 2019-01-22 | 2019-01-22 | Dyeing pen for dyeing biological tissue slices |
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| Publication Number | Publication Date |
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| CN109632435A CN109632435A (en) | 2019-04-16 |
| CN109632435B true CN109632435B (en) | 2024-06-18 |
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| CN201910059112.7A Active CN109632435B (en) | 2019-01-22 | 2019-01-22 | Dyeing pen for dyeing biological tissue slices |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110286022A (en) * | 2019-05-27 | 2019-09-27 | 郑州中普医疗器械有限公司 | Fast constant temperature high definition dyes structural unit and high definition colouring method |
| CN110376046A (en) * | 2019-08-07 | 2019-10-25 | 北京大学人民医院(北京大学第二临床医学院) | Mouse Nerve retrograde tracing marks component |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN210071456U (en) * | 2019-01-22 | 2020-02-14 | 新乡医学院 | A dyeing pen for biological tissue section dyeing |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| TW580376B (en) * | 2002-10-24 | 2004-03-21 | Oncoprobe Biotech Inc | Test pen |
| GB2472330B (en) * | 2005-07-11 | 2011-06-15 | Longhand Data Ltd | Apparatus and methods relating to voting systems |
| KR20110104312A (en) * | 2010-03-16 | 2011-09-22 | 장치식 | Syringe device with pen for drug injection |
| CN103909010B (en) * | 2014-04-15 | 2016-05-04 | 洛阳惠尔纳米科技有限公司 | A kind of mechanical type Magnetic Isolation pen |
| WO2017109201A1 (en) * | 2015-12-24 | 2017-06-29 | Koninklijke Philips N.V. | Device for staining 3d biopsy tissue |
| CN206832814U (en) * | 2017-07-04 | 2018-01-02 | 昆明医科大学第二附属医院 | A kind of novel immune group is drawn a circle pen |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN210071456U (en) * | 2019-01-22 | 2020-02-14 | 新乡医学院 | A dyeing pen for biological tissue section dyeing |
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