CN210103940U - Multipurpose plastic pipette tip for preparing and transferring cell and lipid vesicle suspension - Google Patents
Multipurpose plastic pipette tip for preparing and transferring cell and lipid vesicle suspension Download PDFInfo
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- CN210103940U CN210103940U CN201920296151.4U CN201920296151U CN210103940U CN 210103940 U CN210103940 U CN 210103940U CN 201920296151 U CN201920296151 U CN 201920296151U CN 210103940 U CN210103940 U CN 210103940U
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Abstract
The utility model discloses a multipurpose transfer pipette plastic gun head for preparing suspension of cells and lipid vesicles, which comprises a gun head body and a filter element; the pipette plastic tip avoids the situation of poor accuracy and repeatability caused by sample damage when the pipette tip is not used for pipetting in the prior art in the operation process of preparing and transferring various cell or lipid vesicle (microdroplet) suspensions, and improves the stability of the operation of preparing and transferring the cell or lipid vesicle (microdroplet) suspensions; this liquid-transfering plastic rifle head is when dividing liquid at every turn, and than original classic straight shape rifle head, the operation action accords with the hydromechanics principle more, and the operation process is lighter convenient, is particularly suitable for the experiment that needs various cells, the accurate continuous liquid-transfering operation of fat drop suspension, has avoided conventional liquid-transfering rifle head to contain the sample loss that cell or fat drop suspension caused when handling this type of special, has guaranteed activity and integrality etc. of operation sample such as cell fat drop, has ensured validity, accuracy and the repeatability of experimental result.
Description
Technical Field
The utility model relates to the technical field of biology and medicine, especially multipurpose cell, preparation of lipid vesicle suspension are moved and are got liquid-transfering plastic rifle head.
Background
The liquid transfer operation is one of the most basic operation modes in biomedical activities, the current operation is mainly to transfer various types of related liquids through various liquid transfer devices (guns) matched with liquid transfer device plastic tips, limited by historical factors and traditional habits, the current conventional various liquid transfer plastic tips take various biomolecules or non-biological materials fully dissolved in homogeneous solutions such as water, ethanol and the like as main processing objects, such as various aqueous solutions or various DNA, RNA, protein, various acid and alkali salt solutions, etc. which are fully dissolved in water, wherein the conventional pipetting plastic tip is selected, does not need to consider too much, can meet the pipetting requirements only by ensuring the accuracy and timeliness of the pipetting action of the fully dissolved homogeneous solution, no damage to the sample, good repeatability and the like, these tips therefore tend to be relatively sharp in shape, the tips do not require special treatment and the outlet cross-section is relatively sharp.
However, the solutions to be pipetted in more and more laboratories are not the conventional solutions to be fully dissolved as described above, especially the operations of pipetting, resuspension and the like of various cell suspensions, and more laboratories need to prepare various lipid vesicles (droplets), such as lipid vesicle coating of DNA and RNA molecules in various cell transfections by liposome method, digital PCR, various single-cell lipid-containing droplet operations in single-cell droplet preparation and pipetting in single-cell sequencing, etc., the conventional operations of pipetting cells or lipid-containing vesicles (droplets) in these clinical research and production do not have any specially-matched pipette tips, and only the pipette tips described above for conventional solutions to be fully dissolved in water or ethanol are used to perform the relevant operations, which brings about many problems in practical work, for example, because the conventional pipette tip is relatively sharp and the tip outlet is relatively sharp, a great deal of damage or loss of cell materials or lipid vesicles (droplets) in the pipetting process cannot meet the requirements of actual operation in the scientific research and clinical production processes, because the preparation and pipetting precision of special liquid samples is reduced and the repeatability is poor due to mismatching of the pipette tip, the accuracy and the high efficiency of experimental results cannot be ensured, especially under the condition of high-precision operation, such as treatment of a micro-drop digital PCR chip and preparation of single sample by single cell sequencing, each droplet is expected to have an active single cell, which provides higher requirements for the suspension and operation of the relevant cell and lipid vesicles (droplets) with the preparation meeting the specifications, the existing pipette tip cannot meet the technical development requirements far, and how to solve the problem which needs to be solved urgently, the provision of a more suitable multi-purpose suspension of cells, lipid vesicles (microdroplets) to prepare the pipetting plastic tips is among the most important.
SUMMERY OF THE UTILITY MODEL
1. Technical problem to be solved
The utility model discloses the technical problem that needs to solve lies in, provides multipurpose cell, the preparation of lipid vesicle suspension and moves and get liquid-transfering plastic rifle head, and the shape material of this kind of special liquid-transfering rifle head etc. all carries out special treatment to cell or fat drop operation, has avoided conventional liquid-transfering rifle head to handle the sample loss that this type of special contains cell or fat drop sample time to cause, has guaranteed operation sample's activity and integrality etc. has ensured validity, accuracy and the repeatability of experimental result.
2. Technical scheme
In order to realize the purpose, the technical scheme of the utility model is that: the transfer liquid plastic gun head for preparing the multipurpose cell and lipid vesicle suspension comprises a gun head body and a filter element; the gun head body is of a cavity structure without a top cover; the gun head body consists of an upper cavity and a lower cavity which are integrated; the upper cavity is of a cylindrical structure, the lower cavity is of a conical cylindrical structure, and the axes of the upper cavity and the lower cavity are superposed; the wall thickness H1 of the upper cavity is 1mm, the wall thickness of the lower cavity is gradually reduced from top to bottom, and the wall thickness H2 of the bottom surface of the lower cavity is 0.4 mm; the filter element is a PE filter element and is arranged in the upper cavity; the distance l between the top surface of the filter element and the top surface of the upper cavity is 17 mm.
The pipette tip comprises a lower cavity, an upper cavity, a lower cavity and a pipette tip, wherein the wall thickness of the top surface of the lower cavity is the same as the wall thickness H1 of the upper cavity, and the wall thickness of the lower cavity is linearly reduced from top to bottom.
According to the pipette plastic tip, the outer diameter D of the top surface of the tip body is 7mm, and the inner diameter D of the bottom surface of the tip body is 2 mm.
The length of the upper chamber L1 is 24mm, and the length of the lower chamber L2 is 26 mm.
The pipette tip body is made of one of polypropylene materials, polyethylene materials and polytetrafluoroethylene.
According to the pipette plastic tip, the surface of the outer peripheral wall of the top of the upper cavity is circumferentially provided with the plurality of anti-skid parts, and the anti-skid parts are subjected to frosting treatment; the inner peripheral wall of the top surface of the upper cavity is provided with a plurality of reinforcing rings along the axial direction.
In the pipette plastic tip, the distance h from the bottom surface of the thickened part to the top surface of the tip body is 6 mm.
According to the pipette tip, passivation is performed on the bottom of the inner wall of the upper cavity.
According to the pipette plastic tip, the label is printed on the top surface of the tip body.
According to the pipette plastic tip, the inner wall of the tip body is subjected to protein adsorption removing surface coating treatment.
3. Advantageous effects
To sum up, the beneficial effects of the utility model reside in that:
(1) the utility model discloses a move liquid plastic gun head, in the operation process that moves to various cell or lipid vesicle (droplet) suspension preparation, avoided in the past when not having this kind of special gun head move liquid operation, the sample damage that causes when the accuracy repeatability is poor, improved stability, the high efficiency that this kind of more and more cell or lipid vesicle (droplet) suspension preparation moved and got the operation, especially precious sample and the experiment under the high accuracy requirement, guaranteed that the result of this kind of experiment is reliable;
(2) the utility model discloses a move liquid plastic gun head when dividing liquid at every turn, than original classic straight shape gun head, the operation action accords with the hydrodynamics principle more, operation process is light more convenient, be particularly suitable for the experiment that needs various cells, the accurate continuous liquid operation that moves of fat drop suspension, the sample loss that the conventional liquid gun head caused when handling this type of special cell-containing or fat drop suspension has been avoided, the activity and the integrality etc. of operating sample such as cell fat drop have been guaranteed, the validity, the accuracy and the repeatability of experimental result have been ensured.
Drawings
Fig. 1 is a schematic structural view of a pipette plastic tip of the present invention.
Fig. 2 is a side view of the pipette plastic tip of the present invention.
Fig. 3 is a schematic view of a local structure of a pipette plastic tip a of the present invention.
In the figure: 1-gun head body, 100-upper chamber, 200-lower chamber, 101-antiskid part, 102-reinforcing ring, 2-filter element and 3-label.
Detailed Description
Referring to fig. 1 to 3, a pipette plastic tip comprises a tip body 1 and a filter element 2; the gun head body 1 is of a cavity structure without a top cover; the lance head body 1 consists of an upper cavity 100 and a lower cavity 200 which are integrated; the upper cavity 100 is of a cylindrical structure, the lower cavity 200 is of a conical cylindrical structure, and the axes of the upper cavity and the lower cavity are overlapped; the wall thickness H1 of the upper cavity 100 is 1mm, the wall thickness of the lower cavity 200 is gradually reduced from top to bottom, and the wall thickness H2 of the bottom surface of the lower cavity 200 is 0.4 mm; the filter element 2 is a PE filter element, and the filter element 2 is disposed in the upper chamber 100; the distance l between the top surface of the filter element 2 and the top surface of the upper cavity 100 is 17mm, and the filter element 2 can ensure that when air flow generated by movement of a piston of the liquid transfer device passes through the gun head in the liquid transfer use process, the attached bacteria attached to the part of the liquid transfer device is prevented from being brought into cell lipid droplet suspension in the operation process through the gun head, and the aseptic operation in the liquid droplet preparation and removal process is ensured.
The wall thickness of the top surface of the lower cavity 200 is the same as the wall thickness H1 of the upper cavity 100, the wall thickness of the lower cavity 200 is linearly reduced from top to bottom, the outer diameter D of the top surface of the gun head body 1 is 7mm, the inner diameter D of the bottom surface of the gun head body 1 is 2mm, the length L1 of the upper cavity 100 is 24mm, and the length L2 of the lower cavity 200 is 26 mm. By adopting the structural parameters, the accuracy of sample application action is ensured, the improvement directly causes the possibility of slowing down the flow speed and advection of samples in the liquid transfer operation, prevents various turbulences in the previous operation from occurring, greatly reduces the shearing force generated when liquid flows in the tube cavity, prevents the damage of cells or lipid droplets caused by the shearing force generated by the turbulences, ensures the integrity and the activity of experimental cells or lipid droplet samples, improves the effectiveness, the accuracy and the repeatability of the experiment, and ensures the stability and the reliability of experimental results.
The gun head body 1 is made of one of polypropylene material, polyethylene material or polytetrafluoroethylene, and the three materials are common high polymer materials of medical equipment, so that the gun head has the advantages of being stable in chemistry, transparent and convenient for operators to observe.
A plurality of anti-skid parts 101 are arranged on the surface of the outer peripheral wall of the top of the upper cavity 100 along the circumferential direction, and the anti-skid parts 101 are subjected to frosting treatment; the inner peripheral wall of the top surface of the upper cavity 100 is axially provided with a plurality of reinforcing rings 102, wherein the distance h between the bottom surface of the anti-skid part 101 and the top surface of the gun head body 1 is 6mm, the reinforcing rings 102 on the inner wall of the upper cavity 100 are used for preventing air leakage and pressure leakage caused by untight combination of the gun head and the tip of the pipette in the operation process, the accuracy and the repeatability in the sample operation are ensured, and the thickened part 101 on the outer wall is convenient for manual operation of manually installing the gun head on the tip of the pipette or taking the gun head off the tip of the pipette under a special emergency condition.
The cross section of the bottom of the lower cavity 200 is passivated, so that a sharp cross section is rounded, the sample operation damage caused by the cross section of the gun head body 1 is reduced when cells or lipid drops pass through the tip of the gun head, and the experimental result is ensured.
The top surface of the gun head body 1 is printed with a label 3, so that an operator can conveniently observe the liquid transfer operation.
The inner wall of the gun head body 1 is subjected to deproteinization adsorption surface coating treatment, so that cell membrane or lipid droplet membrane damage caused by conventional gun head inner wall protein adsorption is reduced when cells or lipid droplets pass through the inner wall of the gun head body 1, sample operation damage is reduced, and an experimental result is ensured.
When in use: firstly, the reinforcing ring 102 is sleeved on a pipette gun, the pipette gun is inserted into the upper cavity 100 with the reinforcing ring 102 through the lower end of the pipette gun to complete the fixed installation of the gun head body 1, then, a pipettor is started to generate negative pressure, then cell or lipid droplet suspension is sucked into the lower cavity 200, the upper plane of the suspension is kept below the filter element 2, the pipettor is moved to drive the gun head to reach a suspension release position, then, positive pressure is generated through the pipettor, the cell or lipid droplet suspension in the lower cavity 200 is released, and only flowing pressure air passes through the filter element 2 to realize aseptic pipetting. Practice shows that the pipetting plastic gun head can ensure the accuracy of pipetting spotting action, greatly improve the possibility of slowing and advecting the flow rate of a sample, prevent turbulence generated in the previous operation, greatly reduce the shearing force generated when liquid flows in a tube cavity, prevent the damage of cells or lipid droplets caused by the shearing force generated by the turbulence, ensure the integrity and activity of an experimental cell or lipid droplet sample, improve the effectiveness, accuracy and repeatability of an experiment, and ensure the stability and reliability of an experimental result.
The above only is the preferred embodiment of the present invention, not limiting the patent scope of the present invention, all the equivalent structure changes made by the content of the specification of the present invention, or directly or indirectly applying the technical field attached to other related products, including pipette tips, pipettes, small tubes, etc. of various specifications and sizes, are included in the patent protection scope of the present invention.
Claims (10)
1. Multipurpose preparation of cell, lipid vesicle suspension is moved and is got liquid-transfering plastic rifle head, its characterized in that: comprises a gun head body (1) and a filter element (2); the gun head body (1) is of a cavity structure without a top cover; the lance head body (1) consists of an upper cavity (100) and a lower cavity (200), and the upper cavity and the lower cavity are of an integrated structure; the upper cavity (100) is of a cylindrical structure, the lower cavity (200) is of a conical cylindrical structure, and the axes of the upper cavity and the lower cavity are overlapped; the wall thickness H1 of the upper cavity (100) is 1mm, the wall thickness of the lower cavity (200) is gradually reduced from top to bottom, and the wall thickness H of the bottom surface of the lower cavity (200) is 0.4 mm; the filter element (2) is a PE filter element, and the filter element (2) is arranged in the upper cavity (100); the distance l between the top surface of the filter element (2) and the top surface of the upper cavity (100) is 17 mm.
2. A pipette plastic tip according to claim 1, characterised in that: the wall thickness of the top surface of the lower cavity (200) is the same as that of the wall thickness H1 of the upper cavity (100), and the wall thickness of the lower cavity (200) is linearly reduced from top to bottom.
3. A pipette plastic tip according to claim 1, characterised in that: the external diameter D of the top surface of the gun head body (1) is 7mm, and the internal diameter D of the bottom surface of the gun head body (1) is 2 mm.
4. A pipette plastic tip according to claim 1, characterised in that: the length L1 of the upper cavity (100) is 24mm, and the length L2 of the lower cavity (200) is 26 mm.
5. A pipette plastic tip according to claim 1, characterised in that: the gun head body (1) is made of one of polypropylene material, polyethylene material or polytetrafluoroethylene.
6. A pipette plastic tip according to claim 1, characterised in that: a plurality of anti-skid parts (101) are arranged on the surface of the outer peripheral wall of the top of the upper cavity (100) along the circumferential direction, and the anti-skid parts (101) are subjected to frosting treatment; the inner peripheral wall of the top surface of the upper cavity (100) is provided with a plurality of reinforcing rings (102) along the axial direction.
7. A pipette tip according to claim 6, characterised in that: the distance between the bottom surface of the antiskid part (101) and the top surface of the gun head body (1) is 6 mm.
8. A pipette plastic tip according to claim 1, characterised in that: and passivating the bottom section of the lower cavity (200).
9. A pipette plastic tip according to claim 1, characterised in that: and a label (3) is printed on the top surface of the gun head body (1).
10. A pipette plastic tip according to claim 1, characterised in that: the inner wall of the gun head body (1) is subjected to protein adsorption removal surface coating treatment.
Priority Applications (1)
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CN201920296151.4U CN210103940U (en) | 2019-03-09 | 2019-03-09 | Multipurpose plastic pipette tip for preparing and transferring cell and lipid vesicle suspension |
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CN201920296151.4U CN210103940U (en) | 2019-03-09 | 2019-03-09 | Multipurpose plastic pipette tip for preparing and transferring cell and lipid vesicle suspension |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109706064A (en) * | 2019-03-09 | 2019-05-03 | 刘晓 | Multipurpose cell, the preparation of lipid vesicles suspension pipette liquid relief plastics pipette tips |
CN112827523A (en) * | 2021-01-08 | 2021-05-25 | 上海金鑫生物科技有限公司 | Ultrahigh molecular polyethylene filter containing activated carbon |
-
2019
- 2019-03-09 CN CN201920296151.4U patent/CN210103940U/en active Active
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109706064A (en) * | 2019-03-09 | 2019-05-03 | 刘晓 | Multipurpose cell, the preparation of lipid vesicles suspension pipette liquid relief plastics pipette tips |
CN112827523A (en) * | 2021-01-08 | 2021-05-25 | 上海金鑫生物科技有限公司 | Ultrahigh molecular polyethylene filter containing activated carbon |
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