CN109706064A - Multipurpose cell, the preparation of lipid vesicles suspension pipette liquid relief plastics pipette tips - Google Patents
Multipurpose cell, the preparation of lipid vesicles suspension pipette liquid relief plastics pipette tips Download PDFInfo
- Publication number
- CN109706064A CN109706064A CN201910177396.XA CN201910177396A CN109706064A CN 109706064 A CN109706064 A CN 109706064A CN 201910177396 A CN201910177396 A CN 201910177396A CN 109706064 A CN109706064 A CN 109706064A
- Authority
- CN
- China
- Prior art keywords
- pipette tips
- liquid relief
- cavity
- epicoele
- resorption
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000007788 liquid Substances 0.000 title claims abstract description 59
- 239000004033 plastic Substances 0.000 title claims abstract description 38
- 229920003023 plastic Polymers 0.000 title claims abstract description 38
- 239000000725 suspension Substances 0.000 title claims abstract description 22
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 150000002632 lipids Chemical class 0.000 title claims abstract description 17
- 239000000463 material Substances 0.000 claims description 11
- 238000012545 processing Methods 0.000 claims description 7
- 230000003014 reinforcing effect Effects 0.000 claims description 6
- -1 polypropylene Polymers 0.000 claims description 5
- 239000011248 coating agent Substances 0.000 claims description 4
- 238000000576 coating method Methods 0.000 claims description 4
- 239000004810 polytetrafluoroethylene Substances 0.000 claims description 4
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims description 4
- 239000004743 Polypropylene Substances 0.000 claims description 3
- 238000010521 absorption reaction Methods 0.000 claims description 3
- 230000002093 peripheral effect Effects 0.000 claims description 3
- 229920000573 polyethylene Polymers 0.000 claims description 3
- 229920001155 polypropylene Polymers 0.000 claims description 3
- 210000004027 cell Anatomy 0.000 abstract description 28
- 238000000034 method Methods 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 8
- 238000002474 experimental method Methods 0.000 abstract description 8
- 238000012546 transfer Methods 0.000 abstract description 8
- 230000033001 locomotion Effects 0.000 abstract description 6
- 210000001789 adipocyte Anatomy 0.000 abstract description 2
- 239000012530 fluid Substances 0.000 abstract description 2
- 238000000926 separation method Methods 0.000 abstract description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 238000010008 shearing Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 235000019441 ethanol Nutrition 0.000 description 2
- 239000012456 homogeneous solution Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000013011 mating Effects 0.000 description 2
- 238000004153 renaturation Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- 230000008719 thickening Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 239000005977 Ethylene Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000001447 alkali salts Chemical class 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000001616 monocyte Anatomy 0.000 description 1
- 238000002161 passivation Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 210000005239 tubule Anatomy 0.000 description 1
Abstract
The invention discloses multipurpose cell, the preparations of lipid vesicles suspension to pipette liquid relief plastics pipette tips, including pipette tips ontology and filter core；This liquid relief plastics pipette tips, in the operating process pipetted for various cells or the preparation of lipid vesicles (droplet) suspension, when avoiding in the past without this Special gun head pipetting, caused by exact repetition difference caused by sample damage situation, improve cell or the preparation of lipid vesicles (droplet) suspension pipette the stability of operation；This liquid relief plastics pipette tips are in each liquid separation, than original classical straight pipette tips, operational motion is more in line with fluid mechanics principle, operating process is more easy and convenient, it is particularly suitable for needing the experiment of the accurate continuous pipetting of various cells, fat drips suspension, avoid conventional liquid transfer gun head handle it is such it is special containing cell or fat drips suspension when caused by sample loss, ensure that activity and integrality of the operation sample such as cell fat drips etc., it is ensured that validity, accuracy and the repeatability of experimental result.
Description
Technical field
The present invention relates to biology and medicine technology field, especially multipurpose cell, the preparations of lipid vesicles suspension to pipette
Liquid relief plastics pipette tips.
Background technique
Pipetting is one of mode of operation most basic in biomedical activity, and current operation mainly passes through various
The mating pipettor plastics pipette tips of pipettor (rifle), carry out various types of related liquids and pipette, by historical factor and traditional habit
It is limited, the various liquid relief plastics pipette tips of current routine are all the various biomolecule to be substantially soluble in the homogeneous solutions such as water, ethyl alcohol
Or non-biological material is main process object, such as various aqueous solutions or the various DNA, RNA, albumen, various acid for being substantially soluble in water
Alkali salt solution etc., the conventional liquid relief plastics pipette tips selected here, and do not have to consider too much, to be only the need to ensure that these are sufficiently dissolved
Homogeneous solution pipette the accurate, timely of movement, sample nondestructive, reproducible etc. can meet liquid relief requirement, therefore these
Often shape is more tapering for pipette tips, and tip does not need specially treated, and outlet is than relatively sharp.
But needing the solution for carrying out liquid relief processing in more and more laboratories at present is not that routine as used above is filled
The solution of point dissolution, especially various cell suspensions such as pipette, blow and beat, being resuspended at the operation, and there are also more and more laboratories need to
The preparation for carrying out various lipid vesicles (droplet) carries out the rouge of DNA in various cell transfectings, RNA molecule such as liposome method
Matter vesicle coating, digital pcr, in unicellular sequencing unicellular drop preparation pipette in it is various containing unicellular lipid droplet actuator etc.
Deng the regular growth in the production of, these scientific research clinics or contain the pipetting of lipid vesicles (droplet), at present all without special
The matched liquid transfer gun head of door is operated, and has to appoint the shifting of homogeneous solution in conventional sufficiently dissolution water or in ethyl alcohol on borrowing
Liquid pipette tips carry out relevant operation, and this operation brings many problems in actual operation, such as because of conventional liquid relief pipette tips
It is more tapering, tip outlet than the considerable damage for causing cell material in pipetting processes or lipid vesicles (droplet) such as relatively sharp or
Loss, is not able to satisfy the needs of practical operation in these scientific research clinic production processes, because the mismatch of mating liquid transfer gun head, makes
Accuracy decline, poor repeatability are pipetted at the preparation of these special liquid samples, it cannot be guaranteed that the precise and high efficiency of experimental result, especially
It is in the case where high precision manipulation, as wished each in droplet type digital pcr chip processing, the preparation of unicellular sequencing monocyte sample
There is an activity unicellular in droplet, this meets relevant preparation cell and lipid vesicles (droplet) suspension and the shifting of specification
More stringent requirements are proposed for liquid operation, and existing liquid transfer gun head has been far from satisfying the needs of technology development, how to solve
Certainly this urgent need urgently solves the problems, such as, provides more suitable multipurpose cell, the preparation of lipid vesicles (droplet) suspension pipettes
Liquid relief plastics pipette tips are wherein mostly important a part.
Summary of the invention
1. the technical issues that need to address
The technical problem to be solved in the invention is, provides multipurpose cell, the preparation of lipid vesicles suspension pipettes liquid relief
Plastics pipette tips, shape material of this special liquid transfer gun head etc. have all carried out specially treated for cell or fat drips operation, have kept away
Exempted from conventional liquid transfer gun head handle it is such it is special containing cell or fat drips sample when caused by sample loss, ensure that operation sample
Activity and integrality etc., it is ensured that validity, accuracy and the repeatability of experimental result.
2. technical solution
To achieve the goals above, the technical scheme is that multipurpose cell, the preparation of lipid vesicles suspension pipette
Liquid plastics pipette tips, including pipette tips ontology and filter core；The pipette tips ontology is half-headed cavity body structure；The pipette tips ontology is by upper
Chamber and cavity of resorption composition, and the two is structure as a whole；The epicoele is cylinder-like structure, and the cavity of resorption is cone tube shape structure,
And the two axis is overlapped；The epicoele wall thickness H 1 is 1mm, and the cavity of resorption wall thickness gradually becomes smaller from top to bottom, and cavity of resorption bottom surface
Wall thickness H 2 is 0.4mm；The filter core is PE filter core, and the filter core is arranged described intracavitary；The filter core top surface and institute
Stating the distance between epicoele top surface l is 17mm.
Above-mentioned liquid relief plastics pipette tips, wherein cavity of resorption top surface wall thickness is identical as the epicoele wall thickness H 1, the cavity of resorption
Wall thickness linearly becomes smaller from top to bottom.
Above-mentioned liquid relief plastics pipette tips, wherein the pipette tips body top surface outer diameter D is 7mm, in pipette tips ontology bottom surface
Diameter d is 2mm.
Above-mentioned liquid relief plastics pipette tips, wherein the upper cavity length L1 is 24mm, and the lower cavity length L2 is 26mm.
Above-mentioned liquid relief plastics pipette tips, wherein the pipette tips ontology uses polypropylene material, polythene material or polytetrafluoro
One of ethylene is made.
Above-mentioned liquid relief plastics pipette tips, wherein the epicoele top peripheral wall surface is circumferentially arranged several anti-slip parts,
And the anti-slip part does frosted processing；Epicoele top surface internal perisporium is axially disposed several reinforcing rings.
Above-mentioned liquid relief plastics pipette tips, wherein the thickening part bottom surface is 6mm away from pipette tips body top surface distance h.
Above-mentioned liquid relief plastics pipette tips, wherein the upper cavity wall bottom is passivated processing.
Above-mentioned liquid relief plastics pipette tips, wherein the pipette tips body top surface is printed with label.
Above-mentioned liquid relief plastics pipette tips, wherein the pipette tips inner body wall makees deproteinized absorption surface coating treatment.
3. beneficial effect
In conclusion the beneficial effects of the present invention are:
(1) liquid relief plastics pipette tips of the invention, what is pipetted for various cells or the preparation of lipid vesicles (droplet) suspension
In operating process, when avoiding in the past without this Special gun head pipetting, caused by accuracy weight caused by sample damage
The situation of renaturation difference, improves this kind of more and more cells or the preparation of lipid vesicles (droplet) suspension pipettes the stabilization of operation
Property, high efficiency, the experiment under especially precious sample and high-precision requirement ensure that the result of this kind of experiment is reliable；
(2) for liquid relief plastics pipette tips of the invention in each liquid separation, than original classical straight pipette tips, operational motion is more
Meet fluid mechanics principle, operating process is more easy and convenient, is particularly suitable for needing various cells, fat drips suspension accurately continuous
The experiment of pipetting, avoid conventional liquid transfer gun head handle it is such it is special containing cell or fat drips suspension when caused by sample damage
Lose, ensure that the activity and integrality etc. of the operation sample such as cell fat drips, it is ensured that the validity of experimental result, accuracy with again
Renaturation.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of liquid relief plastics pipette tips of the present invention.
Fig. 2 is a kind of side view of liquid relief plastics pipette tips of the present invention.
Fig. 3 is the partial structural diagram at a kind of liquid relief plastics pipette tips A of the present invention.
In figure: 1- pipette tips ontology, 100- epicoele, 200- cavity of resorption, 101- anti-slip part, 102- reinforcing ring, 2- filter core, 3- mark
Label.
Specific embodiment
It please refers to shown in attached drawing 1 to attached drawing 3, a kind of liquid relief plastics pipette tips, including pipette tips ontology 1 and filter core 2；The pipette tips
Ontology 1 is half-headed cavity body structure；The pipette tips ontology 1 is made of epicoele 100 and cavity of resorption 200, and the two is integrated knot
Structure；The epicoele 100 is cylinder-like structure, and the cavity of resorption 200 is cone tube shape structure, and the two axis is overlapped；On described
100 wall thickness H 1 of chamber is 1mm, and 200 wall thickness of cavity of resorption gradually becomes smaller from top to bottom, and 200 bottom surface wall thickness H2 of cavity of resorption is 0.4mm；
The filter core 2 is PE filter core, and the filter core 2 is arranged in the epicoele 100；2 top surface of filter core and the epicoele 100
The distance between top surface l is 17mm, and the filter core 2 guarantees in liquid relief use process, because pipettor piston motion produces
When raw air-flow passes through the pipette tips, the attached air-flow of bacterium for preventing pipettor part from adhering to brings cell when operation by the pipette tips
It can guarantee that preparation pipettes the sterile working during drop in fat drips suspension.
The 200 top surface wall thickness of cavity of resorption is identical as 100 wall thickness H 1 of epicoele, 200 wall thickness of cavity of resorption line from top to bottom
Property become smaller, the 1 top surface outer diameter D of pipette tips ontology is 7mm, and the 1 bottom surface internal diameter d of pipette tips ontology is 2mm, and the epicoele 100 is long
Degree L1 is 24mm, and the 200 length L2 of cavity of resorption is 26mm.Using above-mentioned structural parameters, guarantee the accurate of point sample movement, it is this
Improve directly contribute pipetting sample flow speed slow down, advection a possibility that greatly enhance, prevent in the past operate in
Various turbulent flows occur, and have been greatly reduced the shearing force generated when liquid flows in lumen, it is therefore prevented that the shearing generated by turbulent flow
Power causes cell or fat drips to damage, and ensure that the integrality and activity of experimental cell or fat drips sample, improves the effective of experiment
Property, accuracy, repeatability, it is ensured that stability, the reliability of experimental result.
The pipette tips ontology 1 is made of one of polypropylene material, polythene material or polytetrafluoroethylene (PTFE), and above three
Kind of material is the high molecular material of common Medical Devices, with chemical stabilization, it is transparent and convenient for the excellent of operator's observation
Point.
The 100 top peripheral wall surface of epicoele is circumferentially arranged several anti-slip parts 101, and the anti-slip part 101 is done
Frosted processing；The 100 top surface internal perisporium of epicoele is axially disposed several reinforcing rings 102, wherein 101 bottom of anti-slip part
1 top surface distance h of identity distance pipette tips ontology is 6mm, the reinforcing ring 102 on 100 inner wall of epicoele for prevent in operating process because
Gas leakage leakage pressure caused by pipette tips are not close in conjunction with pipettor tip, guarantees the accuracy and repeatability when sample operation, outer wall
On thickening part 101 it is convenient under special emergency, pipette tips are installed into pipettor tip or are taken from pipettor tip by hand
Under manual operation.
200 bottom section of cavity of resorption makees Passivation Treatment, and sharp keen section is become mellow and full, guarantees to pass through in cell or fat drips
When pipette tips tip, because sample operation damage reduces caused by 1 section of pipette tips ontology, guarantee the result of experiment.
1 top surface of pipette tips ontology is printed with label 3, when facilitating pipetting, the observation of operator.
1 inner wall of pipette tips ontology makees deproteinized absorption surface coating treatment, guarantees described in pass through in cell or fat drips
When pipette tips 1 inner wall of ontology, cell membrane or fat drips membrane damage are reduced caused by adsorbing because of conventional pipette tips intine-held protein, and sample is caused to be grasped
Making damage reduces, and guarantees the result of experiment.
When use: firstly, the reinforcing ring 102 is sleeved on liquid-transfering gun, and being added by the lower end of liquid-transfering gun insertion band
In the epicoele 100 of strong ring 102, the fixed installation of the pipette tips ontology 1 is completed, then, starting pipettor generates negative pressure, then sucks
Cell or fat drips suspension enter the cavity of resorption 200, and plane on suspension is kept to move described in pipettor drive below the filter core 2
Pipette tips reach at suspension release, then generate positive pressure by pipettor, discharge cell or fat drips suspension in cavity of resorption 200, wherein only
There is the pressure air of flowing by the filter core 2, realizes sterile liquid relief.Practice have shown that using above-mentioned liquid relief plastics pipette tips, energy
Enough guarantee the accurate of liquid relief point sample movement, so that a possibility that sample flow rate slows down, advection greatly enhances, is grasped before preventing
The turbulent flow occurred in work has been greatly reduced the shearing force generated when liquid flows in lumen, it is therefore prevented that because what turbulent flow generated cuts
Shear force causes cell or fat drips to damage, and ensure that the integrality and activity of experimental cell or fat drips sample, improve experiment has
Effect property, accuracy, repeatability, it is ensured that stability, the reliability of experimental result.
The above description is only a preferred embodiment of the present invention, is not intended to limit the scope of the invention, all utilizations
Equivalent structure transformation made by present specification, directly or indirectly with the technology neck for being attached to other Related products
Domain, liquid transfer gun head, pipette, suction pipe, tubule including various specifications size etc. similarly include in patent protection of the invention
In range.
Claims (10)
1. the preparation of multipurpose cell, lipid vesicles suspension pipettes liquid relief plastics pipette tips, it is characterised in that: including pipette tips ontology (1)
With filter core (2)；The pipette tips ontology (1) is half-headed cavity body structure；The pipette tips ontology (1) is by epicoele (100) and cavity of resorption
(200) it forms, and the two is structure as a whole；The epicoele (100) is cylinder-like structure, and the cavity of resorption (200) is taper cone barrel
Shape structure, and the two axis is overlapped；Epicoele (100) wall thickness H 1 be 1mm, cavity of resorption (200) wall thickness from top to bottom by
Gradual change is small, and cavity of resorption (200) bottom surface wall thickness H (2) is 0.4mm；The filter core (2) is PE filter core, and the filter core (2) is arranged
In the epicoele (100)；The distance between filter core (2) top surface and the epicoele (100) top surface l are 17mm.
2. liquid relief plastics pipette tips according to claim 1, it is characterised in that: cavity of resorption (200) the top surface wall thickness with it is described
Epicoele (100) wall thickness H 1 is identical, and cavity of resorption (200) wall thickness linearly becomes smaller from top to bottom.
3. liquid relief plastics pipette tips according to claim 1, it is characterised in that: pipette tips ontology (1) top surface outer diameter D is
7mm, pipette tips ontology (1) the bottom surface internal diameter d are 2mm.
4. liquid relief plastics pipette tips according to claim 1, it is characterised in that: epicoele (100) the length L1 is 24mm, institute
Stating cavity of resorption (200) length L2 is 26mm.
5. liquid relief plastics pipette tips according to claim 1, it is characterised in that: the pipette tips ontology (1) uses polypropylene material
One of material, polythene material or polytetrafluoroethylene (PTFE) are made.
6. liquid relief plastics pipette tips according to claim 1, it is characterised in that: epicoele (100) the top peripheral wall surface
It is circumferentially arranged to have several anti-slip parts (101), and the anti-slip part (101) does frosted processing；Epicoele (100) top surface inner circumferential
Wall is axially disposed several reinforcing rings (102).
7. liquid relief plastics pipette tips according to claim 6, it is characterised in that: anti-slip part (101) bottom surface is away from pipette tips sheet
Body (1) top surface distance is 6mm.
8. liquid relief plastics pipette tips according to claim 1, it is characterised in that: cavity of resorption (100) bottom section is passivated
Processing.
9. liquid relief plastics pipette tips according to claim 1, it is characterised in that: pipette tips ontology (1) top surface is printed with mark
It signs (3).
10. liquid relief plastics pipette tips according to claim 1, it is characterised in that: pipette tips ontology (1) inner wall makees deproteinized
Absorption surface coating treatment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910177396.XA CN109706064A (en) | 2019-03-09 | 2019-03-09 | Multipurpose cell, the preparation of lipid vesicles suspension pipette liquid relief plastics pipette tips |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910177396.XA CN109706064A (en) | 2019-03-09 | 2019-03-09 | Multipurpose cell, the preparation of lipid vesicles suspension pipette liquid relief plastics pipette tips |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109706064A true CN109706064A (en) | 2019-05-03 |
Family
ID=66265843
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910177396.XA Pending CN109706064A (en) | 2019-03-09 | 2019-03-09 | Multipurpose cell, the preparation of lipid vesicles suspension pipette liquid relief plastics pipette tips |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109706064A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113801360A (en) * | 2021-11-18 | 2021-12-17 | 赛宁(苏州)生物科技有限公司 | Surface treatment method for realizing low-adsorption plastic liquid-transfer suction head |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6066297A (en) * | 1997-01-03 | 2000-05-23 | Matrix Technologies Corporation | Small sample volume displacement pipette tips |
| US6117394A (en) * | 1996-04-10 | 2000-09-12 | Smith; James C. | Membrane filtered pipette tip |
| US20120301960A1 (en) * | 2011-05-24 | 2012-11-29 | Brigham Young University | Lance Device and Associated Methods for Delivering a Biological Material Into a Cell |
| CN203112833U (en) * | 2013-01-24 | 2013-08-07 | 中南林业科技大学 | Round-head liquid transfer suction pipe capable of preventing cells from being damaged |
| CN207641491U (en) * | 2017-11-23 | 2018-07-24 | 上海阿趣生物科技有限公司 | A kind of novel liquid-transfering gun suction nozzle |
| CN208583358U (en) * | 2018-08-06 | 2019-03-08 | 刘晓 | Elongated drop prepares liquid relief plastics pipette tips |
| CN210103940U (en) * | 2019-03-09 | 2020-02-21 | 刘晓 | Multipurpose plastic pipette tip for preparing and transferring cell and lipid vesicle suspension |
-
2019
- 2019-03-09 CN CN201910177396.XA patent/CN109706064A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6117394A (en) * | 1996-04-10 | 2000-09-12 | Smith; James C. | Membrane filtered pipette tip |
| US6066297A (en) * | 1997-01-03 | 2000-05-23 | Matrix Technologies Corporation | Small sample volume displacement pipette tips |
| US20120301960A1 (en) * | 2011-05-24 | 2012-11-29 | Brigham Young University | Lance Device and Associated Methods for Delivering a Biological Material Into a Cell |
| CN203112833U (en) * | 2013-01-24 | 2013-08-07 | 中南林业科技大学 | Round-head liquid transfer suction pipe capable of preventing cells from being damaged |
| CN207641491U (en) * | 2017-11-23 | 2018-07-24 | 上海阿趣生物科技有限公司 | A kind of novel liquid-transfering gun suction nozzle |
| CN208583358U (en) * | 2018-08-06 | 2019-03-08 | 刘晓 | Elongated drop prepares liquid relief plastics pipette tips |
| CN210103940U (en) * | 2019-03-09 | 2020-02-21 | 刘晓 | Multipurpose plastic pipette tip for preparing and transferring cell and lipid vesicle suspension |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113801360A (en) * | 2021-11-18 | 2021-12-17 | 赛宁(苏州)生物科技有限公司 | Surface treatment method for realizing low-adsorption plastic liquid-transfer suction head |
| CN113801360B (en) * | 2021-11-18 | 2022-03-04 | 赛宁(苏州)生物科技有限公司 | Surface treatment method for realizing low-adsorption plastic liquid-transfer suction head |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO2016078340A1 (en) | Apparatus, system, and method for dispensing/mixing a small quantity of liquid | |
| US20220023854A1 (en) | Pipette tip extension | |
| He et al. | Technologies and applications in micro-volume liquid handling | |
| Mao et al. | High-precision digital droplet pipetting enabled by a plug-and-play microfluidic pipetting chip | |
| CN210103940U (en) | Multipurpose plastic pipette tip for preparing and transferring cell and lipid vesicle suspension | |
| CN107206291A (en) | The method and system separated for buoyancy | |
| CN109706064A (en) | Multipurpose cell, the preparation of lipid vesicles suspension pipette liquid relief plastics pipette tips | |
| CN106669556A (en) | Method for preparing millimeter-scale granules by changing microfluidic channels | |
| CN216224463U (en) | Device for generating digital PCR (polymerase chain reaction) liquid drops or single-cell liquid drops and liquid drop generating tube | |
| WO2021037218A1 (en) | Sample adding needle for preparing microdroplets and microdroplet preparation method | |
| CN101713002A (en) | Fluorescent quantitative PCR detection method of pathogen nucleic acid by one-step method | |
| JP6556220B2 (en) | Handling of three-phase fluid | |
| CN207913815U (en) | Pipettor gun head | |
| US11491479B2 (en) | Laboratory glassware and associated methods for using the same | |
| CN209564999U (en) | A kind of bent angle liquid relief plastics pipette tips | |
| US20210114024A1 (en) | Device and Method for Preparing Sample Material | |
| CN217973236U (en) | Dropper for embryo culture | |
| CN109550528B (en) | Multi-angle nondestructive observation method and device for internal channel of micro-fluidic chip | |
| CN205020105U (en) | Pasteur pipet | |
| CN210474031U (en) | Aseptic dropper for biological medicine | |
| CN204661699U (en) | A kind of quantitative graduated tube pipetting solution for trace | |
| CN211159828U (en) | Multi-channel bottle mouth liquid separator | |
| CN109499637A (en) | A kind of bent angle liquid relief plastics pipette tips | |
| CN204276007U (en) | A kind of suction nozzle type pipette of improvement | |
| CN218250337U (en) | Temporary straw storage device for laboratory |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |