CN209546606U - A kind of seawater medaka zygotes hatching apparatus - Google Patents

A kind of seawater medaka zygotes hatching apparatus Download PDF

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Publication number
CN209546606U
CN209546606U CN201920112728.1U CN201920112728U CN209546606U CN 209546606 U CN209546606 U CN 209546606U CN 201920112728 U CN201920112728 U CN 201920112728U CN 209546606 U CN209546606 U CN 209546606U
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hatching
inside casing
outer cylinder
larva
juvenile
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CN201920112728.1U
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Chinese (zh)
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宋静静
于道德
盖珊珊
赵文溪
于超勇
刘莹
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Shandong Marine Biology Institute
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Shandong Marine Biology Institute
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The utility model relates to a kind of seawater medaka zygotes hatching apparatus, belong to fish hatching apparatus field, and described device includes hatching outer cylinder, hatching inside casing, hatching stratum reticulare, larva and juvenile travelling outlet, aeration aperture and hook;Multiple hatching stratum reticulares are set inside the hatching inside casing, and every layer is equipped with a larva and juvenile travelling outlet, and for hatching inside casing through hook sleeve in hatching outer cylinder, hatching outer cylinder bottom has aeration aperture, is connected with inflator pump.The utility model is simple, and operation is easy, can exempt during hatch fish roe and change water, damage and interference of the manual operation to fertilized eggs is effectively reduced, and can be good at inhibiting the growth of the microalgaes such as chlorella and diatom, guarantee fertilized eggs surface cleaning in hatching process, improves final hatching rate.

Description

A kind of seawater medaka zygotes hatching apparatus
Technical field
The invention belongs to fish hatching apparatus fields, and in particular to a kind of dress of free-changing water hatching seawater medaka zygotes It sets.
Background technique
Seawater medaka (Oryzias melastigma) is under the jurisdiction of Chordata, spoke fin net-rope, Beloniformes, strange jaw Medaka section, green Medaka category.Its Type of Spawning, which belongs to, typically sticks together demersal egg, with tuna fisheries greenling, flounder flounder class, catfish, Needlefish etc. is similar.Since it has the biological properties such as individual is small, the generation cycle is short, spawning rate is high, the transparent easy observation of ovum, mesh It is preceding to be proposed as a kind of ideal ocean model fingerling, it is widely used in biological development and ecological toxicology research.
However, although seawater medaka has numerous biological superiorities, since its fertilized eggs surface is with a large amount of long and short Different filiform, ovum grain can mutually stick cluster, and thyrsiform is presented around root long ovum silk aggregation and is hung on female reproduction Hole does not disperse falling off, this will lead to fertilized eggs easy hypoxia death in hatching process.At the same time, seawater medaka Incubating oosperm required temperature is higher, and between 26~28 DEG C, 10~14d can just pip.When higher temperature and hatching Length will lead to a large amount of microalgaes (based on chlorella, diatom) and protozoan is attached to fertilized eggs surface, and fertilized eggs is caused to incubate Change the death or difficult dead in hatching later period larva and juvenile broken shell that blacks early period.If dissecting needle is taken to remove or replace daily fresh Filtering sea needs to spend a large amount of manpowers and time, and fertilized eggs subject to damage in stripping process, hatching rate is caused to reduce.Cause How efficiently this, save hatching seawater medaka zygotes as experiment bottleneck urgent need to resolve.
Currently, rarely seen " a kind of recirculated water linked network piece hatching seawater blueness Medaka of patent in relation to the hatching of seawater medaka zygotes The method of fertilized eggs " (notification number: 106172131A).This method mainly describes the fertilization ovum collecting of seawater medaka, circulation Water hatching, Lentic hatching and seed harvest process.Although solving fertilized eggs during recirculated water hatching to be sucked, melt by circulating pump The problems such as hypoxgia, but there are still certain complexity and inefficiencies for experimental implementation.Such as: (1) entire hatching process must protect Card fertilized eggs attach in mesh sheet, need to be through multiple repeated flushing, and the fertilized eggs not sticked would be unavailable for testing, and cause fertilized eggs Waste, and human interference is excessive;(2) hatching process early period (10~12d) need to carry out in recirculated water, the later period (12~14d) It need to go in hydrostatic and carry out.The method requires fish-egg fertilization time excessively high, it is necessary to be same batch, if fish-egg fertilization time It differs greatly, the larva and juvenile first hatched is easy to be washed away by circulating water flow;(3) during recirculated water hatching, to water velocity and Degree of inflation is required to strictly, and otherwise fertilized eggs, which easily fall off in mesh sheet or bring filtration system by recirculated water, leads to death.
Summary of the invention
The utility model is multiple in order to solve existing medaka class method for incubating oosperm and the cumbersome of device, structure The problems such as miscellaneous provide a kind of experimental provision that is efficient, saving hatching seawater medaka zygotes.
The utility model is achieved by the following technical solution:
A kind of seawater medaka zygotes hatching apparatus, corresponding device include hatching outer cylinder, hatching inside casing, hatching stratum reticulare, Larva and juvenile travelling outlet, hook and aeration aperture;Multiple hatching stratum reticulares are set inside the hatching inside casing, and every layer young equipped with a son Fish swimming outlet, hatching inside casing is through hook sleeve in hatching outer cylinder, and hatching outer cylinder bottom has aeration aperture, with inflator pump phase Even.
Further, the hatching outer cylinder is opaque waterproof.Further, the hatching outer cylinder is by polythene material system At.
Further, hatching inside casing is made of wire, impermeable ovum but permeable.
The application method of above-mentioned apparatus is to be fixed on the hatching stratum reticulare of apparatus of the present invention from level to level respectively in hatching inside casing Seawater medaka zygotes are moved about outlet single berth on hatching stratum reticulare by larva and juvenile, then pass through hatching inside casing by portion It hangs in hatching outer cylinder.
The utility model has the following beneficial effects:
1. the utility model setting is simple, operation is easy, can exempt during hatch fish roe and change water, is effectively reduced artificial The damage and interference to fertilized eggs are operated, final hatching rate is improved.
2. utility model device is to the acquisition time of fertilized eggs, spawning number, inflated size, water body flow speed without spy Provisioning request saves manpower and time, in a disguised form simplifies experimental implementation.
3. utility model device can be good at inhibiting the growth of the microalgaes such as chlorella and diatom, guarantee in hatching process Fertilized eggs surface cleaning is conducive to the final broken shell hatching of larva and juvenile.
Detailed description of the invention
Fig. 1 is the overall structural main section view of the utility model: 1, hatching outer cylinder, 2, hatching inside casing 3, hatching stratum reticulare, 4, Larva and juvenile travelling outlet, 5, hook, 6, aeration aperture.
Specific embodiment
The technical solution of the utility model is further explained below by embodiment combination attached drawing, but this is practical new The protection scope of type is not limited in any form by embodiment.
Embodiment 1
A kind of seawater medaka zygotes hatching apparatus, corresponding device include hatching outer cylinder 1, hatching inside casing 2, hatching stratum reticulare 3, larva and juvenile travelling outlet 4, hook 5 and aeration aperture 6;Multiple hatching stratum reticulares are set inside the hatching inside casing, and every layer is equipped with one A larva and juvenile travelling outlet, for hatching inside casing by 5 sets of hook in hatching outer cylinder, hatching 1 bottom of outer cylinder has aeration aperture 6, with Inflator pump is connected.
The hatching outer cylinder be made of polythene material it is opaque waterproof.
Hatching inside casing is made of wire, impermeable ovum but permeable.
The hatching outer cylinder 1 is having a size of length 27cm, the 1500mL polythene drum of diameter 9cm, on 1/2 length direction Bucket wall has light shield layer.
The hatching inside casing 2 is length 20cm, the metal wire cylinder frame of diameter 7cm, permeable but impermeable ovum.
The hatching stratum reticulare 3 is multiple stratum reticulares being arranged parallel to each other, wherein every two layers of stratum reticulare is a closed cell, height For 2cm, stratum reticulare material can be bolting silk or sieve, and mesh size is 20~30 mesh.
Larva and juvenile travelling outlet 4, is every two layers of stratum reticulare central exit, long × wide: 2 × 2cm.The hook 5, is used for Hatching inside casing 2 is hung on into hatching outer cylinder 1.
The aeration aperture 6 is inflated for connecting inflator pump to hatching apparatus.
By the hatching stratum reticulare of the device of that embodiment of the invention respectively be fixed on from level to level hatching inside casing inside, seawater medaka by Smart ovum moves about outlet single berth on hatching stratum reticulare by larva and juvenile with suction pipe, then by hatching inside casing by hanging over hatching In outer cylinder.The not shown obvious green of 10-11d water body or brown, sediments microscope inspection as the result is shown chlorella and frustule quantity < 100cells/mL.Fertilized eggs surface cleanness is high in hatching barrel, and body surface pigment is clear, hardly seen algae attachment.In fertilized eggs Obvious red is presented in medaka heart, and heartbeat and blood flow are high-visible, and fertilization survival rate of ovum is up to 78 ± 6%, most Whole hatching rate is 36 ± 7%.Provided with the Lentic hatching experimental group that bottom is non-aerating while carrying out the embodiment of the present invention, 10-11d water body is presented green or grows a large amount of brown patches in beaker bottom, and microscopic examination result shows that chlorella or diatom quantity can Up to 104~106cells/mL.Lentic hatching fertilized eggs are adhered to by a large amount of microalgaes to be wrapped up, whole to be presented dark brown, body surface pigment and Organ is almost invisible in ovum, and survival rate is only 40 ± 7%, and final hatching rate is only 17 ± 4%, and there are larva and juvenile tail portion brills Out, but the head broken shell difficulty phenomena of mortality.

Claims (4)

1. a kind of seawater medaka zygotes hatching apparatus, it is characterised in that described device includes hatching outer cylinder (1), hatching inside casing (2), hatch stratum reticulare (3), larva and juvenile travelling outlet (4), hook (5) and aeration aperture (6);Setting is multiple inside the hatching inside casing Hatch stratum reticulare, every layer is equipped with a larva and juvenile travelling outlet, and hatching inside casing in hatching outer cylinder, hatches outer cylinder bottom by hook sleeve Portion has aeration aperture, is connected with inflator pump.
2. a kind of seawater medaka zygotes hatching apparatus according to claim 1, it is characterised in that outside the hatching Cylinder is opaque waterproof.
3. a kind of seawater medaka zygotes hatching apparatus according to claim 1, it is characterised in that outside the hatching Cylinder is made of polythene material.
4. a kind of seawater medaka zygotes hatching apparatus according to claim 1, it is characterised in that hatching inside casing is gold Belong to silk to be made, impermeable ovum but permeable.
CN201920112728.1U 2019-01-23 2019-01-23 A kind of seawater medaka zygotes hatching apparatus Active CN209546606U (en)

Priority Applications (1)

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CN201920112728.1U CN209546606U (en) 2019-01-23 2019-01-23 A kind of seawater medaka zygotes hatching apparatus

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Application Number Priority Date Filing Date Title
CN201920112728.1U CN209546606U (en) 2019-01-23 2019-01-23 A kind of seawater medaka zygotes hatching apparatus

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114128652A (en) * 2021-12-02 2022-03-04 大连海洋大学 Black spot medaka embryo hatching device and method
CN114916479A (en) * 2022-06-02 2022-08-19 山东大学 Hatching method for fertilized eggs of ocean medaka

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114128652A (en) * 2021-12-02 2022-03-04 大连海洋大学 Black spot medaka embryo hatching device and method
CN114128652B (en) * 2021-12-02 2023-01-24 大连海洋大学 Black spot medaka embryo hatching device and method
CN114916479A (en) * 2022-06-02 2022-08-19 山东大学 Hatching method for fertilized eggs of ocean medaka

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