CN209443009U - A kind of controllable temperature and the cell recovery instrument with rotatable tray in use - Google Patents
A kind of controllable temperature and the cell recovery instrument with rotatable tray in use Download PDFInfo
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- CN209443009U CN209443009U CN201822125085.2U CN201822125085U CN209443009U CN 209443009 U CN209443009 U CN 209443009U CN 201822125085 U CN201822125085 U CN 201822125085U CN 209443009 U CN209443009 U CN 209443009U
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- China
- Prior art keywords
- cell recovery
- cell
- cryopreservation tube
- rotatable tray
- rotation axis
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Abstract
A kind of controllable temperature and the cell recovery instrument with rotatable tray in use, it is characterised in that: including ontology, cell recovery cavity, cryopreservation tube pallet, the installation of cell recovery cavity bottom has the rotation axis of motor, and rotation axis top is equipped with several connection buckles;Cryopreservation tube pallet inner hollow, hollow space are equipped with several connection rings;Cryopreservation tube pallet, which is equipped with several inner walls and horizontal plane, has the fixation hole of angle;Cell recovery cavity inner wall is equipped with temperature inductor, and cell recovery chamber outer wall is equipped with temperature regulator, and cell recovery cavity bottom is equipped with heater, and temperature regulator is connected with temperature inductor and heater respectively, for stablizing quickly heating to the water in cavity.The utility model can realize recovery efficient to cells frozen storing liquid, quick and stable.
Description
Technical field
The utility model belongs to medical experiment equipment technical field more particularly to a kind of electronic cell resuscitator.
Background technique
Cell recovery is the process opposite with cell cryopreservation, is the process of cell restoration ecosystem, will be frozen in -170
Cell in DEG C liquid nitrogen container below is cultivated or is fed back again after thawing.When being restored to normal temperature state, the form knot of cell
Structure keeps normal, and biochemical reaction can restore.Different from cell cryopreservation, the heating of cell recovery process is fast, prevents from thawing
Moisture enters cell in journey, forms ice crystal, influences cell survival.
When cell recovery, optimum operation is cell freezing pipe to be rapidly heated to 37 DEG C, is allowed to run through cell most easy
Impaired -5 DEG C~0 DEG C recrystallizes to cell damage to avoid ice crystal, leads to cell death.It at the same time will also be thin
Born of the same parents' cryopreservation tube shakes frequently in a water bath, is heated evenly it.And in actual operation, cell recovery is mostly artificial clamping cell
Cryopreservation tube is placed in the artificial water-bath recovery of water-bath, and there are operation amounts for conventional method very little, water temperature is not permanent, it is firmly uneven to rock
The disadvantages of leading to cell uneven heating.
Currently, being mostly two kinds of cell resuscitator in market, the first is the recovery instrument with temperature-controlling system, is made thin
Born of the same parents' cryovial stabilization is rapidly heated;Second for vibration or rotation function cell recovery instrument, make cell freezing pipe by
It is hot uniform.Lacking on the market can guarantee that cell freezing pipe stablizes the cell recovery instrument being rapidly heated with thermally equivalent simultaneously.
Summary of the invention
The technical problem to be solved by the present invention is to fast in order to overcome the prior art that cannot realize that cell freezing pipe is stablized simultaneously
The defect of speed heating and thermally equivalent, provides a kind of controllable temperature and the cell recovery instrument with rotatable tray in use.
The present invention is to solve above-mentioned technical problem by following technical proposals: a kind of controllable temperature and with rotatable tray in use
Cell recovery instrument, comprising: ontology (1), cell recovery cavity (2), cryopreservation tube pallet (8), cell recovery cavity (2) bottom
(5) installation has the rotation axis (3) of motor (12), and rotation axis (3) top is equipped at least two connection buckles;It is described to freeze
Conduit saddle disk (8) inner hollow, hollow space are equipped at least two connection rings;The cryopreservation tube pallet (8) is equipped in several
Wall and horizontal plane have the fixation hole (9) of angle;Cell recovery cavity (2) inner wall is equipped with temperature inductor (7), described
Cell recovery cavity (2) outer wall is equipped with temperature regulator (6), and cell recovery cavity (2) bottom (5) is equipped with heater (4).
The motor (12) is deluge proof motor.
At least two connection buckles and at least two connection rings are respectively on the rotation axis (3) top and the cryopreservation tube pallet
(8) hollow portion is centrosymmetric.
The clamping of the connection buckle (10) and the connection ring (11) realizes that the rotation axis (3) freeze conduit saddle with described
Disk (8) is connected.
The heater (4) is heating wire, is wound on cell recovery cavity (2) bottom (5).
The fixation hole inner wall (13) and horizontal plane keep 10 ° of -60 ° of angles.
The temperature regulator (6) is connected with the temperature inductor (7) and the heater (4) respectively.
The instrument of the utility model can control temperature by temperature regulator, and cell cryopreservation tube stabilization is made to be rapidly heated,
It improves efficiency;This instrument is heated evenly cell cryopreservation tube by the effect of rotation axis, avoids putting the temperature around cryopreservation tube
Sharply decline causes temperature not high enough, and cell freezes again, thus solve the problems, such as cell thaw slowly, cell freezes again;
Fixation hole inner wall and horizontal plane keep 10 ° of -60 ° of angles, can make cells frozen storing liquid and freeze the increase of tube wall contact area, thus more
Quickly reach cell recovery.
Detailed description of the invention
Fig. 1 is the main view of a kind of controllable temperature and the cell recovery instrument with rotatable tray in use of the utility model;
Fig. 2 is the cross-sectional view of a kind of controllable temperature of the utility model and the cell recovery instrument with rotatable tray in use;
Fig. 3 is the top view of a kind of controllable temperature of the utility model and the cell recovery instrument with rotatable tray in use
In figure, 1. ontologies, 2. cell recovery cavitys, 3. rotation axis, 4. heaters, 5. cell recovery instrument bottom surfaces, 6. temperature
Modulator, 7. temperature inductors, 8. cell cryopreservation tube pallets, 9. fixation holes, 10. connection buckles, 11. connection rings, 12. motor,
13. fixation hole inner wall.
Specific embodiment
Cell recovery instrument as shown in the figure, comprising: ontology 1, cell recovery cavity 2, cryopreservation tube pallet 8, the cell are multiple
2 bottom 5 of cavity installation of reviving has the rotation axis 3 of motor 12, and 3 top of rotation axis is set there are two connection buckle 10;The jelly
8 inner hollow of conduit saddle disk is deposited, is set on hollow space there are two connection ring 11;The cryopreservation tube pallet 8 be equipped with several inner walls with
Horizontal plane has the fixation hole 9 of angle;2 inner wall of cell recovery cavity is equipped with temperature inductor 7, the cell recovery chamber
2 outer wall of body is equipped with temperature regulator 6, and 2 bottom 5 of cell recovery cavity is equipped with heater 4, and the temperature regulator 6 is distinguished
It is connected with the temperature inductor 7 and the heater 4.
Entire cell recovery instrument is cylindrical body, and cell recovery cavity 2 is surrounded by ontology 1 and 2 bottom 5 of cell recovery cavity
Space.The rotation axis 3 is located in the cell recovery cavity 2, and 3 bottom of rotation axis connects 2 bottom of cell recovery cavity
Face 5, middle and lower part are equipped with motor 12, and top portion is set there are two connection buckle 10,8 inner hollow of cryopreservation tube pallet, in
Connection ring 11 there are two setting on empty part, two connection buckles 10 and two connection rings 11 are respectively in 3 top of rotation axis and institute
8 hollow portion of cryopreservation tube pallet is stated, is centrosymmetric.By the clamping of the connection buckle 10 and the connection ring 11, described in realization
Rotation axis 3 is connected with the cryopreservation tube pallet 8;The motor 12 is deluge proof motor, it is ensured that in cell recovery cavity 2
Water will not enter inside the motor 12.
The cell cryopreservation tube pallet 8 is by the connection buckle 10 and the clamping of the connection ring 11 and separates, realize from
3 over-assemble of rotation axis and disassembly, to replace cell cryopreservation tube pallet 8;The cell cryopreservation tube pallet 8 is equipped with several
Fixation hole 9, the fixation hole inner wall 13 keep 60 ° of angles with horizontal plane, realize that cells frozen storing liquid heating surface area expands, and guarantee thin
Born of the same parents' recovery fast and stable;The Assurance of Size of the fixation hole 9 can block cell cryopreservation tube pipe lid, it is ensured that the rotation axis 3 rotates
When fix cell cryopreservation tube.
2 bottom 5 of cell recovery cavity is equipped with heater 4, is used for water temperature heating;2 inner wall of cell recovery cavity
Equipped with temperature inductor 7, water temperature is incuded in soaking water;The external wall of the cell recovery chamber 2 is equipped with temperature regulator 6, the temperature
Degree 6 one end of modulator is connected with the temperature inductor 7, and the other end is connected with the heater 4, described for adjusting and heating
Water temperature in cell recovery cavity 2 guarantees the recovery of cells frozen storing liquid fast and stable.
The course of work of the electronic cell recovery instrument of the utility model are as follows: when needing to carry out cell recovery, be filled to cell
In recovery cavity 2, water temperature heating to 37 DEG C, the cell cryopreservation tube of taking-up is put into solid by connect in advance external power supply, heater 4
Determine in hole 9, fixation hole 9 just blocks cryopreservation tube lid at this time, and being obliquely installed for fixation hole inner wall 13 makes cell cryopreservation tube incline
Tiltedly.Then cryopreservation tube pallet 8 is realized from the rotation axis 3 by the clamping of the connection buckle 10 and the connection ring 11
Assembling, is immersed in cell cryopreservation tube lower part in water.After motor 12 is powered, rotation axis 3 drives cryopreservation tube pallet 8 to start to revolve
Turn, so that the cell cryopreservation tube in fixation hole 9 be driven to rotate in water, rotation 1min or so, cell recovery is completed.It is multiple to cell
Su Hou separates the connection buckle 10 and the connection ring 11, and the taking-up of cell cryopreservation tube pallet 8 can be carried out subsequent experimental operation.
If fruit temperature inductor 7 senses that water temperature drop, temperature regulator 6 can continue to control the heating of heater 4, make water temperature all-the-time stable
At 37 DEG C.
Claims (7)
1. a kind of controllable temperature and the cell recovery instrument with rotatable tray in use, it is characterised in that: including ontology (1), cell recovery chamber
Body (2), cryopreservation tube pallet (8), cell recovery cavity (2) bottom (5) installation have the rotation axis (3) of motor (12),
Rotation axis (3) top is equipped at least two connection buckles (10);Cryopreservation tube pallet (8) inner hollow, on hollow space
Equipped at least two connection rings (11);The cryopreservation tube pallet (8), which is equipped with several inner walls and horizontal plane, has the fixation of angle
Hole (9);Cell recovery cavity (2) inner wall is equipped with temperature inductor (7), and cell recovery cavity (2) outer wall is equipped with
Temperature regulator (6), cell recovery cavity (2) bottom (5) are equipped with heater (4).
2. a kind of controllable temperature according to claim 1 and the cell recovery instrument with rotatable tray in use, it is characterised in that: described
Motor (12) is deluge proof motor.
3. a kind of controllable temperature according to claim 1 and the cell recovery instrument with rotatable tray in use, it is characterised in that: connection
Button, respectively on the rotation axis (3) top and the cryopreservation tube pallet (8) hollow portion, is centrosymmetric with connection ring.
4. a kind of controllable temperature according to claim 1 and the cell recovery instrument with rotatable tray in use, it is characterised in that: pass through
The clamping of the connection buckle (10) and the connection ring (11) realizes that the rotation axis (3) is connected with the cryopreservation tube pallet (8)
It connects.
5. a kind of controllable temperature according to claim 1 and the cell recovery instrument with rotatable tray in use, it is characterised in that: described
Heater (4) is heating wire, is wound on cell recovery cavity (2) bottom (5).
6. a kind of controllable temperature according to claim 1 and the cell recovery instrument with rotatable tray in use, it is characterised in that: described
Fixation hole inner wall (13) and horizontal plane keep 10 ° of -60 ° of angles.
7. a kind of controllable temperature according to claim 1 and the cell recovery instrument with rotatable tray in use, it is characterised in that: described
Temperature regulator (6) is connected with the temperature inductor (7) and the heater (4) respectively.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201822125085.2U CN209443009U (en) | 2018-12-18 | 2018-12-18 | A kind of controllable temperature and the cell recovery instrument with rotatable tray in use |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201822125085.2U CN209443009U (en) | 2018-12-18 | 2018-12-18 | A kind of controllable temperature and the cell recovery instrument with rotatable tray in use |
Publications (1)
Publication Number | Publication Date |
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CN209443009U true CN209443009U (en) | 2019-09-27 |
Family
ID=68014437
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN201822125085.2U Active CN209443009U (en) | 2018-12-18 | 2018-12-18 | A kind of controllable temperature and the cell recovery instrument with rotatable tray in use |
Country Status (1)
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CN (1) | CN209443009U (en) |
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2018
- 2018-12-18 CN CN201822125085.2U patent/CN209443009U/en active Active
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GR01 | Patent grant | ||
CP03 | Change of name, title or address |
Address after: 610000 No. 1, 19 / F, building 7, No. 1333, Tianlong Avenue, Jinniu high tech Industrial Park, Chengdu, Sichuan Patentee after: Saidete biopharmaceutical Co.,Ltd. Address before: 650000 No. 1299, 16 tower, West Tower, Xicheng Plaza, Kunming, Yunnan, China. Patentee before: Sidt Biotechnology Development Co.,Ltd. |
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CP03 | Change of name, title or address |