CN209412236U - Cell climbing sheet - Google Patents
Cell climbing sheet Download PDFInfo
- Publication number
- CN209412236U CN209412236U CN201822026648.2U CN201822026648U CN209412236U CN 209412236 U CN209412236 U CN 209412236U CN 201822026648 U CN201822026648 U CN 201822026648U CN 209412236 U CN209412236 U CN 209412236U
- Authority
- CN
- China
- Prior art keywords
- main body
- plate main
- creep plate
- support column
- climbing sheet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a kind of cell climbing sheet, which includes creep plate main body, and the front and back sides of creep plate main body are equipped at least three support columns, and the quantity of the support column in creep plate main body obverse and reverse is different.The application off the beaten trackly improves cell climbing sheet, and in the front and back sides of creep plate main body, the different support column of setting quantity, the quantity by differentiating support column can rapidly and accurately distinguish the front and back sides of cell climbing sheet, avoid confusion, it is ensured that experiment is gone on smoothly.The presence of support column makes between creep plate main body and culture dish or six orifice plate bottom surfaces that there are gaps, allow experimenter rapidly with tweezers clamping or movement, it avoids making creep plate main body be difficult to be tilted with tweezers due to fitting closely with culture dish or six orifice plate bottom surfaces by surface tension of liquid because of creep plate main body, damage of the tweezers to creep plate ontology and attached cell is not only reduced, but also is conducive to improve working efficiency.
Description
Technical field
The utility model belongs to experiment equipment field, and in particular to a kind of cell climbing sheet.
Background technique
Cell climbing sheet is placed in slide in culture solution containing cell, after certain time culture, cell adherent growth
It is formed on slide, in In vitro cell experiments such as histology, immunohistochemistry, frozen section, cell smear, in situ hybridizations
It usually needs to use cell climbing sheet in research, to being deeply of great significance for life medical research.
Traditional cell climbing sheet is disadvantageous in that: cell adherent growth in common culture dish or six orifice plates into
Row, this is resulted in: 1. cell climbing sheet can be usually in close contact with culture dish or six orifice plates, cause taking-up cell climbing sheet more tired
It is difficult；And in the prior art often there is the problems such as meticulous tip, tip band tooth in the ordinary tweezers for clamping cell climbing sheet, easily go out
Now clamping is unstable or clamping force is excessive causes creep plate fragmentation；2. when grown multiple cell climbing sheets in a culture dish,
Multiple cell climbing sheets can usually be superimposed simultaneously phase mutual friction in washing step, cause to the attached cell for being grown in cell climbing sheet surface
Damage, destroys the quality of cell climbing sheet.
To solve the above problems, being innovated in the prior art from two angles.
First, being exclusively used in clamping as the Chinese utility model patent of Patent No. CN201620448189.5 discloses one kind
The tweezers of cell climbing sheet, the intermediate plate which is oppositely arranged by two form, and the intermediate plate includes clamping part, shank and connection
Portion, for the retaining part in the front end of intermediate plate, the shank is located at the middle part of intermediate plate, the connecting portion in the tail end of intermediate plate,
Two intermediate plates are linked together by the interconnecting piece of tail end, wherein the clamping part length of an intermediate plate is longer than the clamping minister of another intermediate plate
Degree, the clamping part to extend out form a crotch towards place intermediate plate opposite side, and the crotch end forms the sharp of blade-like
Sharp edge edge, cell climbing sheet is levered up under the cell climbing sheet to protrude into culture dish bottom surface, and the opposite face of two intermediate plate clamping parts is constituted
The clamping face of tweezers, the clamping face of the non-bulging intermediate plate clamping part where it in the crotch end are normal so as not to influence tweezers
Clamp cell climbing sheet.
It is climbed second, such as disclosing a kind of cell application No. is the Chinese utility model patent application of CN200520079192.6
The dedicated culture of piece and washing ware, the culture and washing ware include culture dish, and the center of culture dish and edge are equipped with creep plate slot, creep plate
The slot bottom of slot is lower than culture dish bottom surface 2-4mm, and the slot bottom of creep plate slot is formed with for lifting the linear or dotted prominent of cell climbing sheet
It rises, high about 1-2mm.
As it can be seen that being usually all from improvement culture dish or to improve tweezers in the prior art in order to ensure the quality of cell climbing sheet
Come what is realized, however those skilled in the art are carried out for culture dish or tweezers it should be understood that the core of cell climbing sheet is slide
Improvement for improve conventional efficient produce little effect.Firstly, improving the at high cost of culture dish or tweezers, and improved culture
Ware or tweezers can only be exclusively used in cell climbing sheet, but no longer be applicable in other occasions；Secondly, with cover with the cell climbing sheet of cell into
When row cellular immunofluorescence is tested, because experimental procedure is more, the front and back sides of cell climbing sheet are easily mixed up in experimentation, are caused
Creep plate easy to damage of shuffling during dyeing, mounting and microscopy；And above-mentioned improved culture dish or tweezers are climbed for distinguishing cell
The front and back sides of piece have no benefit, cannot effectively improve the working efficiency and experiment success rate of experimenter.
Utility model content
Present invention purpose be to provide it is a kind of convenient for clamping and easily distinguishable front and back sides cell climbing sheet.
For achieving the above object, the technical solution of the application is as follows:
The front and back sides of a kind of cell climbing sheet, including creep plate main body, the creep plate main body are equipped at least three support columns,
It is different with the quantity of the support column in creep plate main body reverse side in creep plate main body front.
The application off the beaten trackly improves cell climbing sheet, the different branch of setting quantity in the front and back sides of creep plate main body
Dagger, the quantity by differentiating support column can rapidly and accurately distinguish the front and back sides of cell climbing sheet, avoid confusion, it is ensured that experiment
It goes on smoothly.
Also, the presence of support column makes between creep plate main body and culture dish or six orifice plate bottom surfaces there are gap, so that real
The personnel of testing can avoid making creep plate main body and training by surface tension of liquid because of creep plate main body rapidly with tweezers clamping or movement
It supports ware or six orifice plate bottom surfaces fits closely and are difficult to be tilted with tweezers, not only reduce tweezers to the damage of creep plate ontology and attached cell
Wound, and be conducive to improve working efficiency.
It needs creep plate main body face down being incubated for primary antibody, secondary antibody and mounting in immunofluorescence experiment operating process,
The presence of at least three support columns can not only provide stable support for creep plate main body, avoid damage to thin in creep plate main body
Born of the same parents；And the positive cell of creep plate main body can obtain sufficient, uniform antibody incubation, and the dosage of antibody is greatly reduced (existing
It is the antibody that 20 μ L or so are first dripped on sealed membrane when having progress antibody incubation in technology, it is then again that cell climbing sheet front is straight
It connects and is pressed on sealed membrane, carry out antibody incubation.Under this incubation mode, area that not only cell climbing sheet front is contacted with antibody compared with
It is small, and the contact condition of cell climbing sheet center and periphery and antibody is inconsistent；In order to make the positive central location of cell climbing sheet
Antibody can be come into full contact with, it is also necessary to increase the dosage of antibody, and this can be further aggravated cell climbing sheet center and periphery with
The inconsistent situation of the contact condition of antibody)；Be conducive to improve working efficiency, shorten experimental period, saves experimental cost.
In above-mentioned cell climbing sheet, there are three support column, institutes for the circumferential equably setting in the front of the creep plate main body
There are four support columns for the circumferential equably setting of the reverse side for the creep plate main body stated；In the positive support column of creep plate main body in climbing
The support column of piece main body reverse side, which misplaces, to be arranged.The presence of support column can reduce the cell growth area of creep plate main body, therefore be arranged
The premise of support column is to reduce to creep plate body table support column as much as possible while providing stable support for creep plate main body
The occupancy of face area.
By the support column dislocation arrangement of creep plate main body front and back sides convenient for stacking cell climbing sheet, it is empty to reduce cell climbing sheet occupancy
Between while, avoid cell climbing sheet when stacking because contact area is smaller occur displacement cause breakage.
In above-mentioned cell climbing sheet, the creep plate main body is rounded, and the support column is in creep plate main body
Edge.The edge that round creep plate main body is arranged in support column is used for carefully as much as possible also for by creep plate main body center portion
Born of the same parents' adherent growth.
In above-mentioned cell climbing sheet, set-up mode of the support column in creep plate main body may is that the support column is viscous
It is attached in creep plate main body；Or the support column and creep plate main body are integrally formed and are arranged.
In above-mentioned cell climbing sheet, the height of the support column is 1mm.The height of support column to overcome liquid enough
Surface tension avoids fitting closely between creep plate main body and culture dish or six orifice plate bottom surfaces, and excessively high support column is not easy to
The preservation of cell climbing sheet.
In above-mentioned cell climbing sheet, the support column in creep plate main body obverse and reverse has different color marks.
So that being more clear for the resolution of creep plate main body front and back sides.
In above-mentioned cell climbing sheet, the outer peripheral edge of the creep plate main body has wear-resisting bound edge, the wear-resisting bound edge
With U-shaped section, U-shaped wear-resisting bound edge extends to creep plate main body reverse side through creep plate outer peripheral edge from creep plate main body front.Work as use
When tweezers clamp creep plate main body, wear-resisting bound edge avoids tweezers from causing to wear to creep plate main body；It is wear-resisting and relative to creep plate main body
Bound edge is less suitable for cell adherent growth, therefore tweezers clamp wear-resisting bound edge and will not cause to damage to cell.
In above-mentioned cell climbing sheet, wear-resisting bound edge can be set to the entire outer peripheral edge for being wrapped in creep plate main body, but excellent
Selection of land, the wear-resisting bound edge wrap up the local location of the outer peripheral edge of the creep plate main body.It can so be stayed for cell adherent growth
Enough spaces out.
It is small in the thickness of the either side of the creep plate main body, the wear-resisting bound edge in above-mentioned cell climbing sheet
In the height of support column；Also, the inscribed circle that the forward position of the wear-resisting bound edge is surrounded not across support column.So that wear-resisting
Still there are gaps between bound edge and culture dish or six orifice plate bottom surfaces, clamp convenient for tweezers；Reduce wear-resisting packet as much as possible simultaneously
The cladding area on side reserves enough spaces for cell adherent growth.
Compared with prior art, the utility model has the following beneficial effects:
(1) the application off the beaten trackly improves cell climbing sheet, and in the front and back sides of creep plate main body, setting quantity is different
Support column, the quantity by differentiating support column can rapidly and accurately distinguish the front and back sides of cell climbing sheet, avoid confusion, it is ensured that
Experiment is gone on smoothly.
(2) presence of support column makes between creep plate main body and culture dish or six orifice plate bottom surfaces there are gap, so that experiment
Personnel can avoid making creep plate main body and culture by surface tension of liquid because of creep plate main body rapidly with tweezers clamping or movement
Ware or six orifice plate bottom surfaces fit closely and are difficult to be tilted with tweezers, not only reduce tweezers to the damage of creep plate ontology and attached cell
Wound, and be conducive to improve working efficiency.
(3) it needs creep plate face down being incubated for primary antibody, secondary antibody and mounting in immunofluorescence experiment operating process, until
The presence of few three support columns can provide stable support for creep plate main body, avoid damage to climb again while reducing antibody dosage
Cell in piece main body is conducive to improve working efficiency, shortens experimental period, saves experimental cost.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of cell climbing sheet of the utility model；
Fig. 2 is a kind of positive structural schematic diagram of cell climbing sheet of the utility model；
Fig. 3 is a kind of structural schematic diagram of cell climbing sheet reverse side of the utility model；
Fig. 4 is A-A cross-sectional view in Fig. 2；
Fig. 5 is a kind of usage state diagram of cell climbing sheet of the utility model.
Specific embodiment
The technical solution of the utility model is described in further details with reference to the accompanying drawings and detailed description.
Embodiment 1
As shown in Figure 1 and Figure 2 and Figure 3, a kind of cell climbing sheet of the present embodiment, including creep plate main body 1, the creep plate of the present embodiment
Main body 1 with a thickness of the transparent circular glass of 1.7mm by being made；The front and back sides of creep plate main body 1 are equipped at least three support columns 2,
With ensure no matter front or reverse side downward, support column 2 can to creep plate main body 1 formed stablize support；And for the ease of
1 front and back sides of creep plate main body are distinguished, the quantity of the support column 2 of front and back sides should be arranged to different；While in order to subtract
Few influence of the support column 2 to cell adherent growth area in creep plate main body 1, in the present embodiment, the front of creep plate main body 1 is circumferential equal
It is arranged evenly there are three support column 2, circumferentially equably there are four support columns 2 for setting for the reverse side of the creep plate main body 1；In climbing
The positive support column 2 of piece main body 1 misplaces with the support column 2 in 1 turn-over of creep plate main body to be arranged.Dislocation arrangement is convenient for climbing in cell
Piece stacks, reduce cell climbing sheet occupied space, avoid cell climbing sheet when stacking because contact area is smaller occurs shift cause
It is damaged.
As shown in Figures 2 and 3, support column 2 is the marginal position that creep plate main body 1 is arranged in, and the height of support column 2 is equal
For 1mm.
In order to ensure the connectivity robustness between support column 2 and creep plate main body 1, support column 2 and creep plate master in the present embodiment
Body 1 is to be integrally formed setting.Also, the support column 2 in 1 obverse and reverse of creep plate main body has different color marks,
So that being more clear for the resolution of 1 front and back sides of creep plate main body.The set-up mode multiplicity of color mark, such as can be by creep plate
The pigment of the end face of the positive support column 2 of main body 1 or periphery wall coating lemon yellow, by the support column 2 of 1 reverse side of creep plate main body
The pigment of end face or periphery wall coating green, pigment should select recognizable and will not influence the type that experiment carries out.
As shown in Figure 4, in conjunction with Fig. 1-3 as it can be seen that the outer peripheral edge of creep plate main body 1 has wear-resisting bound edge 3, which has
There is U-shaped section, U-shaped wear-resisting bound edge 3 extends to 1 reverse side of creep plate main body through creep plate outer peripheral edge from 1 front of creep plate main body；Work as use
When tweezers clamp creep plate main body 1, wear-resisting bound edge 3 avoids tweezers from causing to wear to creep plate main body 1；And relative to creep plate main body 1,
Wear-resisting bound edge 3 is less suitable for cell adherent growth, therefore tweezers clamp wear-resisting bound edge 3 and will not cause to damage to cell.
Also, the height of support column 2 is respectively less than in the thickness of the either side of creep plate main body 1, wear-resisting bound edge 3；So that
Still there are gaps between wear-resisting bound edge 3 and culture dish or six orifice plate bottom surfaces, clamp convenient for tweezers.
Wear-resisting bound edge 3 can be set to the entire outer peripheral edge for being wrapped in creep plate main body 1, but wear-resisting bound edge 3 in the present embodiment
The local location of the outer peripheral edge of the creep plate main body 1 is wrapped up, so can reserve enough spaces for cell adherent growth.Therefore,
The forward position 31 of wear-resisting bound edge 3 should not cross the inscribed circle 21 that support column 2 surrounds.
As shown in figure 5, then pressing before use, carried out disinfection by x ray irradiation x or autoclave sterilization method to cell climbing sheet
Cell climbing sheet is assigned into inside corresponding sterile sterilizing box according to same direction, when assigning, pays attention to for wear-resisting bound edge 3 being emerging in
Outside, for use.
In use, experimenter holds tweezers, it is clamped at wear-resisting bound edge 3, cell climbing sheet is clamped to tissue culture plate
In hole 4, by cell climbing sheet band there are three support column 2 and support column 2 in lemon yellow one side upward, as front, routinely
Operation carries out cell climbing sheet culture；Cultivate a period of time after, be clamped at wear-resisting bound edge 3 with tweezers, by cell climbing sheet just towards
On be transferred in new cultivation plate hole, carry out immunofluorescence experiment, the specific steps are as follows: PBS clean 5min × 3 time, 4% poly first
The fixed 15min of aldehyde prepares wet box simultaneously with 15cm Tissue Culture Dish；0.5%Triton X-100 penetrating 10min, PBS cleaning
5min × 3 time；After normal two antiserums closing 30min, in the primary antibody working solution for preparing 20 μ L in wet box, the cell that will have been closed
Creep plate takes out, and face down is covered in the wet box that drop has 20 μ L primary antibody working solutions, acts on 1h；Cell climbing sheet is taken out, face-up
It carries out PBS and cleans 5min × 3 time；Again by face down, covers and be incubated for 1h on fluorescence secondary antibody working solution；Cell climbing sheet is taken out, just
PBS is carried out up cleans 5min × 3 time；After DAPI redyes 5min, mounting is in glass slide.
As it can be seen that the cell climbing sheet of the present embodiment had both avoided regular growth creep plate in antibody incubation and mounting process because of cell
The excessively adherent injury that missing is scratched to cellular layer of coating, and the cell of entire level is made to obtain sufficient, uniform antibody incubation.
For experimenter, quickly accurately clamping cell climbing sheet provides clamping site (i.e. wear-resisting bound edge 3) for the use of the cell climbing sheet,
And the probability that can reduce cell specimen damage makes the cell on entire cell climbing sheet receive homogenization processing, saves experiment
Reagent guarantees that the offers such as quality of experiments are convenient.The utility model device is easily fabricated, low in cost, easy to operate, improves section
It grinds and the working efficiency of medical worker, saving experiment reagent guarantees quality of experiments.
Claims (10)
1. a kind of cell climbing sheet, including creep plate main body (1), which is characterized in that the front and back sides of the creep plate main body (1) are equipped with
At least three support columns (2), the quantity in creep plate main body (1) front and the support column (2) in creep plate main body (1) reverse side is not
Together.
2. cell climbing sheet as described in claim 1, which is characterized in that the front of the creep plate main body (1) is circumferential equably
There are three support column (2) for setting, and there are four support column (2) for the circumferential equably setting of the reverse side of the creep plate main body (1)；It is in
Creep plate main body (1) positive support column (2) misplaces with the support column (2) in creep plate main body (1) reverse side and arranges.
3. cell climbing sheet as described in claim 1, which is characterized in that rounded, the described support of the creep plate main body (1)
Column (2) is in the edge of creep plate main body (1).
4. cell climbing sheet as described in claim 1, which is characterized in that the support column (2) is adhered to creep plate main body (1)
On.
5. cell climbing sheet as described in claim 1, which is characterized in that the support column (2) and creep plate main body (1) one at
Type setting.
6. cell climbing sheet as described in claim 1, which is characterized in that the height of the support column (2) is 1mm.
7. the cell climbing sheet as described in any one of claim 1-6, which is characterized in that in creep plate main body (1) front and
The support column (2) of reverse side has different color marks.
8. cell climbing sheet as described in claim 1, which is characterized in that the outer peripheral edge of the creep plate main body (1) is with wear-resisting
Bound edge (3), the wear-resisting bound edge (3) have U-shaped section, and U-shaped wear-resisting bound edge (3) is from creep plate main body (1) front through creep plate
Main body (1) outer peripheral edge extends to creep plate main body (1) reverse side.
9. cell climbing sheet as claimed in claim 8, which is characterized in that the wear-resisting bound edge (3) wraps up the creep plate main body (1)
Outer peripheral edge local location.
10. cell climbing sheet as claimed in claim 8, which is characterized in that described in the either side of the creep plate main body (1)
The thickness of wear-resisting bound edge (3) be respectively less than the height of support column (2)；
Also, the inscribed circle (21) that the forward position (31) of the wear-resisting bound edge (3) is surrounded not across support column (2).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822026648.2U CN209412236U (en) | 2018-12-04 | 2018-12-04 | Cell climbing sheet |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822026648.2U CN209412236U (en) | 2018-12-04 | 2018-12-04 | Cell climbing sheet |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209412236U true CN209412236U (en) | 2019-09-20 |
Family
ID=67936487
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201822026648.2U Active CN209412236U (en) | 2018-12-04 | 2018-12-04 | Cell climbing sheet |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209412236U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111500454A (en) * | 2020-04-29 | 2020-08-07 | 盛小龙 | Cell slide, cell slide pickup device, cell culture plate and application method |
-
2018
- 2018-12-04 CN CN201822026648.2U patent/CN209412236U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111500454A (en) * | 2020-04-29 | 2020-08-07 | 盛小龙 | Cell slide, cell slide pickup device, cell culture plate and application method |
| CN111500454B (en) * | 2020-04-29 | 2023-07-21 | 湖南省肿瘤医院 | Cell climbing sheet, cell climbing sheet pickup device, cell culture plate and application method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN209412236U (en) | Cell climbing sheet | |
| CN110923141A (en) | Improved six-hole plate and cell immunofluorescence staining method based on same | |
| CN108893316A (en) | A kind of extrusion type quantitative anti-skid visible surface sampling culture dish | |
| CN108841676A (en) | One kind splitting formula leakproof quantitative anti-skid visible surface sampling culture dish out | |
| CN108865798A (en) | One kind splitting formula quantitative anti-skid visible surface sampling culture dish out | |
| US10988720B1 (en) | Peel plate assembly | |
| CN108865863A (en) | One kind splitting formula leakproof out and easily marks anti-skidding fan-shaped count table surface sample culture dish | |
| CN108893279A (en) | One kind splitting formula anti-slip visible surface sampling culture dish out | |
| CN108865844A (en) | One kind splitting the visual fan-shaped count table surface sample culture dish of formula quantitative anti-skid out | |
| CN108865864A (en) | One kind splitting the quantitative fan-shaped count table surface sample culture dish of easily label of formula leakproof out | |
| CN108841627A (en) | A kind of extrusion type leakproof anti-slip visible surface sampling culture dish | |
| CN108841611A (en) | A kind of extrusion type leakproof quantitative anti-skid surface sampling culture dish | |
| CN108841670A (en) | One kind splitting formula leakproof out and easily marks anti-skidding criss-cross method surface sampling culture dish | |
| CN108865808A (en) | One kind split out formula quantitative anti-skid visually easily press surface sampling culture dish | |
| CN108893322A (en) | A kind of extrusion type leakproof quantitative visual surface sampling culture dish | |
| CN108865799A (en) | One kind splitting formula leakproof anti-slip visible surface sampling culture dish out | |
| CN108893365A (en) | One kind splitting formula leakproof out and increases quantitative visual surface sampling culture dish | |
| CN108998360A (en) | A kind of extrusion type leakproof quantitative anti-skid visible surface sampling culture dish | |
| CN108865716A (en) | One kind splitting the quantitative easily label criss-cross method surface sampling culture dish of formula leakproof out | |
| CN108893311A (en) | One kind splitting formula anti-slip visible out and holds surface sampling culture dish | |
| CN108865713A (en) | One kind splitting the visual criss-cross method surface sampling culture dish of formula quantitative anti-skid out | |
| CN108841589A (en) | A kind of quantitative fan-shaped count table surface sample culture dish of extrusion type | |
| CN108865845A (en) | One kind splitting formula leakproof out and easily marks visual fan-shaped count table surface sample culture dish | |
| CN108893353A (en) | One kind splitting formula out and easily marks anti-skidding hand-held fan-shaped count table surface sample culture dish | |
| CN108865728A (en) | One kind splitting formula leakproof out and easily marks the easily fan-shaped count table surface sample culture dish of pressure |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |