CN108841589A - A kind of quantitative fan-shaped count table surface sample culture dish of extrusion type - Google Patents

A kind of quantitative fan-shaped count table surface sample culture dish of extrusion type Download PDF

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Publication number
CN108841589A
CN108841589A CN201810894758.2A CN201810894758A CN108841589A CN 108841589 A CN108841589 A CN 108841589A CN 201810894758 A CN201810894758 A CN 201810894758A CN 108841589 A CN108841589 A CN 108841589A
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China
Prior art keywords
wall
ware
ware downside
downside wall
buckle
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Withdrawn
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CN201810894758.2A
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Chinese (zh)
Inventor
韩涛
韩德礼
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Individual
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Individual
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Priority to CN201810894758.2A priority Critical patent/CN108841589A/en
Publication of CN108841589A publication Critical patent/CN108841589A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/10Petri dish
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/02Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention discloses a kind of quantitative fan-shaped count table surface sample culture dishes of extrusion type, the culture dish includes ware bottom and ware lid, ware bottom is equipped in the second buckle and the first buckle for stablizing embedded structure, embedded structure can unclamp when stressed, relatively move movable ware downside wall with the first ware downside wall, graduation mark can indicate the volume of pouring into of agar medium, and radioactive ray can simplify the counting process of bacterium colony.

Description

A kind of quantitative fan-shaped count table surface sample culture dish of extrusion type
Technical field
The present invention relates to a kind of experiment equipment, the quantitative fan-shaped count table surface sample culture dish of especially a kind of extrusion type.
Background technique
Culture dish is one of most widely used experiment equipment in biology, is usually used in the objects such as packaging material for food, tableware The culture of body surface microorganism is tested, and the sampling of body surface microorganism usually wipes determinand body surface with sterile cotton swab Cotton swab or filter paper are perhaps transferred in buffer with aseptic filter paper abutting object under test surface later and are eluted, to reach by face To the purpose of sampling.But cotton swab or filter paper can not discharge microorganism collected completely, be easy to cause Bacterial diversity Incomplete culture;In addition the sampling of body surface microorganism needs sterile working, and cotton swab or filter paper are carrying out microorganism Acquisition and elution during, be easy pollution by environmental microorganism, be extremely difficult to the requirement of sterile working;These disadvantages It will cause the deviation of body surface microorganism testing result.In addition, the operating process of the above method is cumbersome, inefficiency, hold Easily cause the waste of human resources.When the culture dish pours into agar medium, the volume of pouring into of culture medium is usually operator Depending on empirically, therefore the agar medium of different personnel pours into volume and may have larger difference, to influence relevant experiment As a result.When the colony counts for the target culture that the culture medium in culture dish is grown are huge, the counting process of bacterium colony then compares It is cumbersome.
Summary of the invention
For disadvantages mentioned above, the purpose of the present invention is to provide a kind of extrusion types that structure is simple and convenient to operate quantitatively to fan Shape count table surface sample culture dish.The implementation of the culture dish can simplify the sampling process of body surface microorganism, improve test As a result accuracy improves working efficiency.
Fan-shaped count table surface sample culture dish quantitative for extrusion type, to reach the purpose, the technical side that the present invention uses Case is:A kind of quantitative fan-shaped count table surface sample culture dish of extrusion type, including the ware lid that ware bottom and relaxation are covered on ware bottom, It is characterized in that:The ware lid includes tegmental wall and at least one surround the ware lid side wall holded up from tegmental wall, and the ware bottom includes Bottom wall surround the first ware downside wall holded up and at least one parallel sleeve in first ware bottom side from bottom wall at least one Movable ware downside wall on the outside of wall;The first ware downside wall be equipped at least one circle second buckle, second snap collar around Described first ware downside wall one week, the circle plane of second buckle was parallel with the plane of the bottom wall;The movable ware Downside wall is equipped at least one the first buckle of circle, and around described movable ware downside wall one week, described first buckled first snap collar Circle plane it is parallel with the plane of the bottom wall;First buckle and second buckle can be closely fitted into, with It maintains to be in the technical characteristic above the first ware downside wall without activity ware downside wall described under external force;When along certainly living Dynamic ware downside wall is to the direction of the first ware downside wall, when applying force to the movable ware downside wall, first buckle and The embedded structure of second buckle can unclamp, and movable ware downside wall can move in parallel along the direction of the first ware downside wall at this time It is dynamic;The diameter of the ware lid side wall is greater than the first ware downside wall;The diameter of the bottom wall is greater than the tegmental wall;The activity The sum of the height of the height of ware downside wall and the first ware downside wall, less than the height of the ware lid side wall;Described first Ware downside wall is additionally provided at least one graduation mark, and the graduation mark surround the first ware downside wall one week, the circle of the graduation mark Shape plane is parallel with the plane of the bottom wall;In the bottom wall, bottom wall side is extended to from bottom wall central point equipped at least two The bottom wall is divided into the fan-shaped region of at least two area equations by the radioactive ray of edge, the radioactive ray.
Compared with the prior art, the beneficial effects of the invention are as follows:The structure of the culture dish is simple and convenient to operate, can be with The process for simplifying sampling microorganisms from surface of object overcomes the problems, such as to be easy to be polluted by environmental microorganism in sampling process, mention simultaneously High test result accuracy and working efficiency, graduation mark can indicate the volume of pouring into of agar medium, and radioactive ray can simplify The counting process of bacterium colony.
Detailed description of the invention
Illustrate technical solution of the present invention in order to clearer, it below will be to attached drawing needed in embodiment description Make brief introduction, it should be apparent that, drawings in the following description are only some embodiments of the invention, general for this field For logical technical staff, without creative efforts, it can also be obtained according to these attached drawings other attached drawings.
Fig. 1 is the structural schematic diagram of the quantitative fan-shaped count table surface sample culture dish of extrusion type;Fig. 2 is provided at the bottom wall The schematic diagram of radioactive ray;In figure:
1 is ware lid, and 2 be ware bottom, and 3 be tegmental wall, and 4 be bottom wall, and 5 be ware lid side wall, and 7 be the first ware downside wall, and 71 be movable ware bottom Side wall, 72 be the first buckle, and 73 be the second buckle, and 22 be graduation mark, and 23 be radioactive ray.
Specific embodiment
It is right below in conjunction with the drawings and specific embodiments in order to be more clear the objectives, technical solutions, and advantages of the present invention The present invention is described in further detail.It should be appreciated that described embodiment is only used to explain the present invention, it is all to be based on Simplified or equivalent variations, all belongs to the scope of protection of the present invention made by technical solution of the present invention.
In the discussion of specific embodiment, the shape of the quantitative fan-shaped count table surface sample culture dish of the extrusion type is preferable Select it is round, still, those skilled in the art should be understood that it is suitable for such as ellipse, rectangle or its It is suitable for the shape of culture dish.
The invention discloses a kind of quantitative fan-shaped count table surface sample culture dishes of extrusion type, including ware bottom 2 and relaxation covering In the ware lid 1 on ware bottom 2, the ware lid 1 includes tegmental wall 3 and at least one surround the ware lid side wall 5 holded up, institute from tegmental wall 3 The ware bottom 2 stated includes bottom wall 4 and at least one surround the first ware downside wall 7 and at least one parallel sleeve holded up from bottom wall 4 Movable ware downside wall 71 in 7 outside of the first ware downside wall;The diameter of the ware lid side wall 5 is greater than the movable ware bottom side Wall 71;The bottom wall 4 is identical as the shape of the tegmental wall 3.The first ware downside wall 7 is equipped at least one the second buckle of circle 73, second buckle 73 was around described first ware downside wall 7 one weeks, the circle plane of second buckle 73 and the bottom wall 4 plane is parallel;The movable ware downside wall 71 is equipped at least one the first buckle 72 of circle, and first buckle 72 is around described The circle plane of movable ware downside wall 71 1 weeks, first buckle 72 is parallel with the plane of the bottom wall 4;First card Button 72 and second buckle 73 can be closely fitted into, to maintain to be in institute without activity ware downside wall 71 described under external force State the technical characteristic of 7 top of the first ware downside wall;When the direction along certainly movable 71 to the first ware downside wall 7 of ware downside wall, apply When adding active force to the movable ware downside wall 71, the embedded structure of first buckle 72 and second buckle 73 can pine It opens, movable ware downside wall 71 can be moved in parallel along the direction of the first ware downside wall 7 at this time.Features described above has determined the extrusion The basic structure of the quantitative fan-shaped count table surface sample culture dish of formula, wherein the first ware downside wall 7, movable ware downside wall 71 and bottom wall 4 spaces formed are solidified when cultivating inner cavity by the enough agar mediums of injection to the culture medium as culture inner cavity Afterwards, the movable ware downside wall 71 is applied force to, keeps movable ware downside wall 71 parallel along the direction of the first ware downside wall 7 It is mobile, until the plane of movable ware downside wall 71 is lower than the plane of the agar medium, by the agar culture when use Base plane is directly covered in object under test surface, that is, can reach the purpose of sampling microorganisms from surface of object, avoid using cotton swab Son or filter paper carry out the tedious steps eluted again after microorganism acquisition, greatly simplify the mistake of sampling microorganisms from surface of object Journey, and overcome and be easy the problem of being polluted by environmental microorganism in sampling process, at the same improve test result accuracy and Working efficiency.
In the embodiment of the quantitative fan-shaped count table surface sample culture dish of the extrusion type, the diameter of the bottom wall 4 is greater than The diameter of the tegmental wall 3, the setting of this feature can be limited when ware lid 1 and the fastening of ware bottom 2, and ware lid side wall 5 can only be supported in Bottom wall 4.In addition, the height of the activity ware downside wall 71 and the sum of the height of the first ware downside wall 7, are less than the ware lid The height of side wall 5, this feature can limit when ware lid 1 and the fastening of ware bottom 2 that there are one between activity ware downside wall 71 and tegmental wall 3 Fixed gap prevents the agar medium from contacting with tegmental wall 3.
The first ware downside wall 7 is additionally provided at least one graduation mark 22, and the graduation mark 22 is around the first ware bottom Side wall 7 one weeks, the circular flat of the graduation mark 22 was parallel with the plane of the bottom wall 4;The graduation mark 22 can indicate Agar medium pours into volume, to avoid pouring into the difference of volume because of personal experience's difference bring.
In the bottom wall 4, the radioactive ray 23 at 4 edge of bottom wall are extended to from 4 central point of bottom wall equipped at least two, it is described Radioactive ray 23 bottom wall 4 is divided into the fan-shaped regions of at least two area equations;When the culture medium institute in culture dish When the colony counts of the target culture of growth are huge, the bacterium colony count results in a fan-shaped region can be used, multiplied by corresponding Multiple(In embodiment shown in Fig. 2, bottom wall 4 is equally divided into the fan-shaped region of 8 area equations, so multiple is 8)With It indicates all clump counts of culture dish, therefore simplifies the counting process of bacterium colony.
Above by description of listed embodiment, the basic ideas and basic principles of the present invention are elaborated.Obviously, institute The embodiment of description is only a part of the embodiments of the present invention, and non-limiting exhaustion.Based on the embodiments of the present invention, originally Field those of ordinary skill all other embodiment obtained under the premise of not making the creative labor, belongs to this hair Bright protection scope.

Claims (1)

1. a kind of quantitative fan-shaped count table surface sample culture dish of extrusion type, including the ware lid that ware bottom and relaxation are covered on ware bottom, It is characterized in that:The ware lid includes tegmental wall and at least one surround the ware lid side wall holded up, the ware bottom packet from tegmental wall It includes bottom wall and surround the first ware downside wall holded up and at least one parallel sleeve at first ware bottom from bottom wall at least one The movable ware downside wall of side-wall outer side;The first ware downside wall is equipped at least one the second buckle of circle, second snap collar Around described first ware downside wall one week, the circle plane of second buckle was parallel with the plane of the bottom wall;The activity Ware downside wall is equipped at least one the first buckle of circle, and around described movable ware downside wall one week, described first blocked first snap collar The circle plane of button is parallel with the plane of the bottom wall;First buckle and second buckle can be closely fitted into, To maintain to be in the technical characteristic above the first ware downside wall without activity ware downside wall described under external force;When along certainly Movable ware downside wall is to the direction of the first ware downside wall, and when applying force to the movable ware downside wall, described first is buckled It can be unclamped with the embedded structure of second buckle, movable ware downside wall can be parallel along the direction of the first ware downside wall at this time It is mobile;The diameter of the ware lid side wall is greater than the first ware downside wall;The diameter of the bottom wall is greater than the tegmental wall;The work The height of dynamic ware downside wall and the sum of the height of the first ware downside wall, less than the height of the ware lid side wall;Described One ware downside wall is additionally provided at least one graduation mark, and the graduation mark is surround the first ware downside wall one week, the graduation mark Circular flat is parallel with the plane of the bottom wall;In the bottom wall, bottom wall is extended to from bottom wall central point equipped at least two The bottom wall is divided into the fan-shaped region of at least two area equations by the radioactive ray at edge, the radioactive ray.
CN201810894758.2A 2018-08-08 2018-08-08 A kind of quantitative fan-shaped count table surface sample culture dish of extrusion type Withdrawn CN108841589A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810894758.2A CN108841589A (en) 2018-08-08 2018-08-08 A kind of quantitative fan-shaped count table surface sample culture dish of extrusion type

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810894758.2A CN108841589A (en) 2018-08-08 2018-08-08 A kind of quantitative fan-shaped count table surface sample culture dish of extrusion type

Publications (1)

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CN108841589A true CN108841589A (en) 2018-11-20

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Application publication date: 20181120