CN209412216U - A kind of coliform detection device - Google Patents
A kind of coliform detection device Download PDFInfo
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- CN209412216U CN209412216U CN201822213214.3U CN201822213214U CN209412216U CN 209412216 U CN209412216 U CN 209412216U CN 201822213214 U CN201822213214 U CN 201822213214U CN 209412216 U CN209412216 U CN 209412216U
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- bag
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- luer
- water intaking
- coliform
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Abstract
The utility model relates to technical field of microbial detection, more particularly to a kind of coliform detection device, coliform detection device includes water intaking bag, water intaking bag joint assembly and detection bag, bag joint assembly of fetching water includes conduit, it further include the water intaking bag connector and Luer pin end for being respectively arranged on conduit both ends, when detection, silica gel valve at the water intaking bag connector and bag interface of fetching water, which cooperates, to be tightly connected, detection bag check valve on the Luer pin end and detection bag is tightly connected, by water sample, culture carries out the detection of coliform after being transferred in detection bag and detecting medium mixing in water intaking bag under full closeding state, it is easy to operate, good airproof performance, avoid the pollution that may occur in detection process, obtain accurate detection result.
Description
Technical field
The utility model belongs to technical field of microbial detection, and in particular to a kind of coliform detection device.
Background technique
Under microgravity environment, the pathogenic enhancing of coliform, it is easier to lead to generation (the Chin J of disease
Aerospace Med, June 2010, Vol.21, No.2, p144-147), therefore coliform is detected and prevented in time
It controls extremely important.
2016, NASA underwent an at least week using a kind of acquisition and culture for holding mini sequenator and passing through sample
Test period, coliform detection is carried out to sample to be tested, however this method is complicated for operation, and time-consuming.
The common detection method of coliform has zymolyte method and MTF method, and zymolyte method testing principle is coliform
Group's growth in chromogenic culture medium generates enzyme, and the chromogen substrate in decomposition chromogenic culture medium releases chromogen and makes culture medium face
Color changes, and detection method is convenient and efficient, and false positive is low, is applicable in a large amount of samples and quickly detects, common chromogenic culture medium has
The MMO-MUG culture medium of LECC culture medium and commercialization, detection process is simple and quick, and testing result is accurate；MTF method is
The feature that acid produces gas is produced according to coliform energy lactose fermenters, so that the detection of color change occurs for acid-base indicator in culture medium
Method；These detection methods can quickly and easily detect coliform, however under the non-clean environment of microgravity, detection process
Middle sample collection, transfer, culture, inevitably occur open-sky technique, cause to pollute, so that testing result is inaccurate, because
This needs to provide the device being adapted with environment, rapidly and accurately to detect coliform.
The coliform detection device research that the prior art is directed under the non-clean environment of microgravity is very few, especially prevents from examining
Survey process contamination, easy to operate, the accurate coliform detection device of result is even more to be difficult to find that.
Utility model content
Therefore, the technical problem to be solved by the present invention is to overcome the prior art under the non-clean state of microgravity
Coliform detects complicated for operation, vulnerable to pollution and leads to the technical problem of result inaccuracy, provides a kind of non-in microgravity
The coliform detection that sample is transferred in detection bag under full closeding state under clean environment and is cultivated after culture medium mixing
Device, the device is easy to operate, is avoided that pollution to obtain accurate detection result.
The utility model provides a kind of coliform detection device, including with lower component,
Bag is detected, the detection bag includes detecting bag body and the Luer female connector set on detection bag body one end, the detection
It is the test chamber suitable for storage detection medium in bag body, is connected with the inspection for allowing sample to flow into detection bag on the Luer female connector
Survey bag check valve；
Water intaking bag joint assembly, including conduit, further include the water intaking bag connector and Luer public access for being respectively arranged on conduit both ends
Head；
Bag of fetching water seals at the water intaking bag interface including bag body and the water intaking bag interface in water intaking bag body of fetching water
It is fixed with flexible silica gel valve
When the coliform detection device is assembled, the water intaking bag connector and silica gel valve are cooperatively connected, and the Luer is public
Connector and the detection bag check valve are cooperatively connected.
Preferably, the detection medium is colour developing dehydrated medium, and the volume of the detection bag body is 100-500ml, institute
The volume fraction of the weight and the detection bag body of stating detection medium is (2.3g~2.8g): 100ml.
Preferably, the colour developing dehydrated medium is selected from MMO-MUG dehydrated medium, LECC dehydrated medium and lactose egg
One of white peptone dehydrated medium is pre-installed in the test chamber of detection bag body when preparation detects bag.
Preferably, the detection bag further includes the Luer public affairs cap stopper by protecting rope to be connected on the Luer female connector,
Before the assembly of coliform detection device, the Luer public affairs cap stopper is connect with the detection bag check valve, is carried out to the detection bag
Seal protection.
Preferably, the water intaking bag connector and the Luer pin end pass through the side for being interference fitted and being aided with glue reinforcing
Formula is connected with the internal diameter at conduit both ends respectively, magnitude of interference >=0.6mm.
Preferably, the water intaking bag joint assembly further includes water intaking bag joint protective sleeve and mother's Luer cap stopper, is passed through respectively
Protection rope is connect with water intaking bag joint assembly, before coliform detection device assembly, the water intaking bag joint protective sleeve
Protection is sealed to water intaking bag connector, mother's Luer cap stopper is sealed protection to the Luer pin end.
Preferably, the detection bag check valve and the Luer pin end, the detection bag check valve and the Luer are public
Cap stopper, the Luer pin end and mother's Luer cap stopper, its connection type are to be connected and sealed simultaneously by Luer taper
Matching thread positioning.
Preferably, the Luer female connector is bonded using screw thread locking with glue dual solid with the detection bag check valve
Determine mode.
The operating method of the utility model the following steps are included:
S1 injects water sample to be measured into water intaking bag by silica gel valve；
S2 screws off mother's Luer cap stopper on water intaking bag joint assembly Luer pin end, then screws off on detection bag check valve
Luer public affairs cap stopper will test bag check valve and connect with Luer pin end；
S3 removes water intaking bag joint protective sleeve, and water intaking bag connector is passed through silica gel valve and is inserted into containing water sample to be measured water intaking bag
In, water sample to be measured in water intaking bag is transferred in detection bag by squeezing, after water sample has shifted, water intaking bag connector is extracted, screws off
Luer pin end, using respective cap stopper by closure of openings；
S4 shakes the detection bag containing water sample, dissolves detection medium in it all, after 37 DEG C of culture 18-28h, passes through sight
The color change for examining culture obtains the testing result of coliform.
The technical solution of the utility model has the advantages that
1, coliform detection is carried out to water sample under microgravity environment to realize, is connected using water intaking bag joint assembly sealing
Water bag and detection bag are picked up, silica gel valve when detection, at the water intaking bag connector of water intaking bag joint assembly one end and bag interface of fetching water
Cooperation is tightly connected, and the detection bag check valve on the Luer pin end and detection bag of the bag joint assembly other end of fetching water, which seals, to be connected
It connects, by water sample, culture carries out coliform after being transferred in detection bag and detecting medium mixing in water intaking bag under full closeding state
The detection of group, it is easy to operate, the pollution that may occur in detection process is avoided, accurate detection result can be obtained.
2, the water intaking bag connector and conduit connection of water intaking bag joint assembly one end, which use to be interference fitted and be aided with glue, adds
It is tightly connected Gu being realized using the elasticity of conduit, water intaking bag connector is according to the silica gel valve size design at water intaking bag interface, detection
When will water intaking bag connector insertion silica gel valve in be tightly connected, when unassembled be equipped with water intaking bag joint protective sleeve, prevent from assembling preceding quilt
Pollution, guarantees the accuracy of testing result.
3, the Luer pin end of the water intaking bag joint assembly other end and conduit connecting pin using interference fit and are aided with glue
Reinforce, prevent from coming off, to reduce the disengaging risk of product and ensure sealed reliable, when detection Luer pin end with to detect bag unidirectional
Valve junction, and in mother's Luer cap stopper for being equipped with before assembly and Luer pin end junction, except being carried out by Luer taper
Sealed connection is outer to go back matching thread positioning, so that connection is more sealed and consolidated.
4, one end of the Luer female connector of detection bag and detection bag body connection is made jointly by the way that interference fit and glue are Nian Jie
With avoiding leaking, the other end locks the dual fixation side Nian Jie with glue using included screw thread with detection bag check valve junction
Formula realizes the sealed connection of No leakage, and before assembly, detection bag check valve and the connecting pin for bag joint assembly of fetching water are additionally provided with
Luer public affairs cap stopper is sealed, and is sealed guarantor to the cultivating system after the detection medium and injection water sample pre-installed before detection
Shield, prevents pollution, to obtain accurate detection result.
5, the male cap stopper of Luer, mother's Luer cap stopper, water intaking bag joint protective sleeve are equipped with protection rope, so that these parts are micro-
Loss will not be drifted under gravity environment.
Detailed description of the invention
It, below will be right in order to illustrate more clearly of specific embodiment of the present invention or technical solution in the prior art
Specific embodiment or attached drawing needed to be used in the description of the prior art are briefly described, it should be apparent that, it is described below
In attached drawing be that some embodiments of the utility model are not paying creativeness for those of ordinary skill in the art
Under the premise of labour, it is also possible to obtain other drawings based on these drawings.
Fig. 1 is that the present embodiment coliform detection device assembles pre-structure schematic diagram；
Fig. 2 is structural schematic diagram after the assembly of the present embodiment coliform detection device；
Fig. 3 is the region Fig. 1 A structural schematic diagram.
Fig. 4 is the present embodiment Luer female connector structural schematic diagram
Description of symbols:
1, bag is detected；11, bag body is detected；12, Luer female connector；121, protrusion is connected；13, bag check valve is detected；14,
Luer public affairs cap stopper；2, water intaking bag joint assembly；21, water intaking bag connector；22, conduit；23, Luer pin end；24, water intaking bag connector
Protective case；25, mother's Luer cap stopper；3, water intaking bag；31, water intaking bag body；32, water intaking bag interface；33, silica gel valve；4, protection rope.
Specific embodiment
The technical solution of the utility model is clearly and completely described below in conjunction with attached drawing, it is clear that described
Embodiment is the utility model a part of the embodiment, instead of all the embodiments.Based on the embodiments of the present invention, originally
Field those of ordinary skill every other embodiment obtained without making creative work belongs to practical
Novel protected range.
In addition, as long as technical characteristic involved in the utility model different embodiments disclosed below is each other
Not constituting conflict can be combined with each other.
Embodiment 1
As shown in Figs 1-4, a kind of coliform detection device is present embodiments provided, including,
Bag 1 is detected, the detection bag 1 includes detecting bag body 11 and the Luer female connector 12 set on detection 11 one end of bag body,
The detection bag check valve 13 for allowing sample to flow into detection bag 1 is connected on the Luer female connector 12, the detection bag body 11 is adopted
With PVC composite material, there is stronger chemical-resistant stability and elasticity modulus, flexibility, detection bag 1 is suitable for sealing up for safekeeping as one
The container for detecting medium, the test chamber of detection medium and sample to be tested mixing and culture observation storage culture, detects bag
11 edge of body integrally can ensure that internal detection medium 2 years or more and not reveal, volume is designed as 100ml, makes using sealing
Used time directly filling water sample to be measured, without carrying out the measurement of filling amount of liquid, wherein the detection medium is colour developing dry powder culture
Base MMO-MUG；
The present embodiment detection medium is loaded in advance in detection bag body (11), and manufacturing process is that will test a bag EO sterilizing (ring
Oxidative ethane sterilizing) after, MMO-MUG dehydrated medium is distributed into sterile inspection under relative humidity≤50%R.H. clean environment
It surveys in bag 1, then flattens bag body, be stained with the non-threaded end of Luer female connector 12, Luer female connector 12 and detection 11 phase of bag body
One end even is connected by interference fit and the bonding collective effect of glue with detection bag body 11, avoids revealing, junction is equipped with
Prevent the connection protrusion 121 to fall off, the weight 2.7g of the MMO-MUG dehydrated medium.
Water intaking bag joint assembly 2, including conduit 22, further include 21 He of water intaking bag connector for being respectively arranged on 22 both ends of conduit
Luer pin end 23, the water intaking bag joint assembly 2 connection water intaking bag 3 and detection bag 1 provide totally enclosed transfer sample
Channel；
Water intaking bag 3, the water intaking bag interface 32 including water intaking bag body 31 and in water intaking bag body 31, the water intaking bag interface
Sealing is fixed with flexible silica gel valve 33 at 32, injects water sample to be measured to water intaking bag 3 before detection, and when detection does not need power
Water sample to be measured can be squeezed into detection bag 1 in the state of totally-enclosed by manual squeezing by device；
Will affect the recall rate of coliform due to containing micro silver ion in water sample to be measured, water intaking bag 3 be added silver from
Sub- neutralizer sodium thiosulfate；
When the coliform detection device is assembled, the water intaking bag connector 21 is cooperatively connected with silica gel valve 33, described
Luer pin end 23 is connected with the detection bag check valve 13 cooperation.
Silica gel valve 33 has elasticity, so that the junction of water intaking bag connector 21 and silica gel valve 33 is completely enclosed, prevents from revealing
And pollution；Detection bag check valve 13 is realized after being installed in place and the leak-free connection of Luer female connector 12, is saving and is cultivating
Period avoids dry powder or liquid in bag from overflowing outward, while allowing to be detected water sample and flowing into detection bag 1.
The detection bag 1 further includes the Luer public affairs cap stopper 14 being connected on Luer female connector 12 by protection rope 4, described
It is connect before the assembly of coliform detection device with the detection bag check valve 13, protection is sealed to the detection bag 1.
The conduit 22 has elasticity, and the conduit 22 uses high-elastic transparent PVC material, transparency with higher and
Elasticity, function are connection water intaking bag connector 21 and Luer pin end 23, the water intaking bag connector 21 and the Luer pin end
23 are connected with the internal diameter at 22 both ends of conduit respectively by way of being interference fitted and being aided with glue and reinforce, magnitude of interference 0.6mm,
Carry out no less than 0.6mm when other embodiments.
The Luer pin end 23 and 22 connecting pin of conduit use taper fit and avoid disengaging, institute by right angle closing in
It states water intaking bag connector 21 to be designed according to 33 size of silica gel valve at water intaking bag interface 32, the water intaking bag joint assembly 2 is also
Including water intaking bag joint protective sleeve 24 and mother's Luer cap stopper 25, it is connect respectively by protection rope 4 with water intaking bag joint assembly 2,
Before the coliform detection device assembly, 24 pairs of water intaking bag connectors 21 of the water intaking bag joint protective sleeve are sealed protection,
The risk of unnecessary contact bring exogenous pollution is avoided, mother's Luer cap stopper 25 carries out the Luer pin end 23 close
Envelope connection is avoided in product after packaging bag taking-up, contaminated before assembling.
The Luer pin end 23 and the detection bag check valve 13, the Luer pin end 23 and mother's Luer cap stopper
25, the detection bag check valve 13 and described 14 its connection type of Luer public affairs cap stopper are to be connected and sealed by Luer taper
And matching thread positions.
The Luer female connector 12 is with the detection bag check valve 13 using the dual fixation of screw thread locking and glue bonding
Mode.
Using the present embodiment carry out coliform detection operating method the following steps are included:
S1 injects water sample to be measured into water intaking bag by silica gel valve；
S2 screws off mother's Luer cap stopper on water intaking bag joint assembly Luer pin end, then screws off on detection bag check valve
Luer public affairs cap stopper will test bag check valve and connect with Luer pin end；
S3 removes water intaking bag joint protective sleeve, and water intaking bag connector is passed through silica gel valve and is inserted into containing water sample to be measured water intaking bag
In, water sample to be measured in water intaking bag is transferred in detection bag by squeezing, after water sample has shifted, water intaking bag connector is extracted, screws off
Luer pin end, using respective cap stopper by closure of openings；
S4 shakes the detection bag containing water sample, dissolves detection medium in it all, after 37 DEG C of culture 18-28h, passes through sight
The color change for examining culture obtains the testing result of coliform.
Embodiment 2
A kind of coliform detection device is present embodiments provided, wherein detection bag volume is 200ml, detects and is pre-installed in bag
The LECC dehydrated medium of 5.6g, the present embodiment rest part are as described in Example 1.
Embodiment 3
A kind of coliform detection device is present embodiments provided, wherein detection bag volume is 500ml, detects and is pre-installed in bag
The lactose protein peptone dehydrated medium of 11.5g, the present embodiment rest part are as described in Example 1.
Obviously, the above embodiments are merely examples for clarifying the description, and does not limit the embodiments.It is right
For those of ordinary skill in the art, can also make on the basis of the above description it is other it is various forms of variation or
It changes.There is no necessity and possibility to exhaust all the enbodiments.And it is extended from this it is obvious variation or
It changes among the protection scope created still in the utility model.
Claims (8)
1. a kind of coliform detection device, which is characterized in that including with lower component,
It detects bag (1), detection bag (1) includes detection bag body (11) and the Luer female connector for being set to detection bag body (11) one end
(12), it is the test chamber suitable for storage detection medium in detection bag body (11), is connected on the Luer female connector (12)
Sample is allowed to flow into the detection bag check valve (13) of detection bag (1)；
It fetches water bag joint assembly (2), including conduit (22), further includes the water intaking bag connector (21) for being respectively arranged on conduit (22) both ends
With Luer pin end (23)；
It fetches water bag (3), including water intaking bag body (31) and the water intaking bag interface (32) being set in water intaking bag body (31), the water intaking bag
Sealing is fixed with flexible silica gel valve (33) at interface (32)；
When the coliform detection device is assembled, the water intaking bag connector (21) and silica gel valve (33) are cooperatively connected, described
Luer pin end (23) and the detection bag check valve (13) are cooperatively connected.
2. coliform detection device according to claim 1, which is characterized in that the detection medium is colour developing dry powder training
Base is supported, the volume of detection bag body (11) is 100-500ml, weight and detection bag body (11) of the detection medium
Volume fraction is (2.2g~2.8g): 100ml.
3. coliform detection device according to claim 2, which is characterized in that the colour developing dehydrated medium is selected from
One of MMO-MUG dehydrated medium, LECC dehydrated medium or lactose protein peptone dehydrated medium detect bag in preparation
(1) it is pre-installed on when in the test chamber of detection bag body (11).
4. coliform detection device according to claim 1, which is characterized in that the detection bag (1) further includes passing through
Protection rope (4) is connected to the Luer public affairs cap stopper (14) on the Luer female connector (12), before the assembly of coliform detection device, institute
It states Luer public affairs cap stopper (14) to connect with the detection bag check valve (13), protection is sealed to the detection bag (1).
5. coliform detection device according to claim 1, which is characterized in that the water intaking bag connector (21) and described
Luer pin end (23) is connected with the internal diameter at conduit (22) both ends by way of being interference fitted and being aided with glue and reinforce respectively,
Magnitude of interference >=0.6mm.
6. coliform detection device according to claim 1, which is characterized in that the water intaking bag joint assembly (2) is also
Including water intaking bag joint protective sleeve (24) and mother's Luer cap stopper (25), pass through protection rope (4) and water intaking bag joint assembly (2) respectively
Connection, the coliform detection device assembly before, the water intaking bag joint protective sleeve (24) to fetch water bag connector (21) into
Row seal protection, mother's Luer cap stopper (25) are sealed protection to the Luer pin end (23).
7. described in any item coliform detection devices according to claim 1~6, which is characterized in that the detection bag is unidirectional
Valve (13) and the Luer pin end (23), the detection bag check valve (13) and the Luer public affairs cap stopper (14), the Luer
Pin end (23) and mother's Luer cap stopper (25), its connection type are to be connected and sealed by Luer taper and cooperate spiral shell
Line positioning.
8. described in any item coliform detection devices according to claim 1~6, which is characterized in that the Luer female connector
(12) with detection bag check valve (13) using the dual fixed form of screw thread locking and glue bonding.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822213214.3U CN209412216U (en) | 2018-12-26 | 2018-12-26 | A kind of coliform detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822213214.3U CN209412216U (en) | 2018-12-26 | 2018-12-26 | A kind of coliform detection device |
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| Publication Number | Publication Date |
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| CN209412216U true CN209412216U (en) | 2019-09-20 |
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ID=67939320
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| Application Number | Title | Priority Date | Filing Date |
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| CN201822213214.3U Active CN209412216U (en) | 2018-12-26 | 2018-12-26 | A kind of coliform detection device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109576148A (en) * | 2018-12-26 | 2019-04-05 | 中国航天员科研训练中心 | A kind of coliform detection device and its detection method |
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2018
- 2018-12-26 CN CN201822213214.3U patent/CN209412216U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109576148A (en) * | 2018-12-26 | 2019-04-05 | 中国航天员科研训练中心 | A kind of coliform detection device and its detection method |
| CN109576148B (en) * | 2018-12-26 | 2024-05-14 | 中国航天员科研训练中心 | Coliform group detection device and detection method thereof |
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