CN109576148B - Coliform group detection device and detection method thereof - Google Patents
Coliform group detection device and detection method thereof Download PDFInfo
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- CN109576148B CN109576148B CN201811602734.1A CN201811602734A CN109576148B CN 109576148 B CN109576148 B CN 109576148B CN 201811602734 A CN201811602734 A CN 201811602734A CN 109576148 B CN109576148 B CN 109576148B
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- 238000001514 detection method Methods 0.000 title claims abstract description 182
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 159
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- 239000000843 powder Substances 0.000 claims abstract description 25
- 239000002609 medium Substances 0.000 claims abstract description 15
- 238000012258 culturing Methods 0.000 claims abstract description 12
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims abstract description 7
- 239000008101 lactose Substances 0.000 claims abstract description 7
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- 108010080698 Peptones Proteins 0.000 claims abstract description 6
- 235000019319 peptone Nutrition 0.000 claims abstract description 6
- 238000007789 sealing Methods 0.000 claims description 25
- 241000894006 Bacteria Species 0.000 claims description 22
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 22
- 239000000741 silica gel Substances 0.000 claims description 22
- 229910002027 silica gel Inorganic materials 0.000 claims description 22
- 238000000034 method Methods 0.000 claims description 21
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- 238000001125 extrusion Methods 0.000 claims description 6
- 238000004026 adhesive bonding Methods 0.000 claims description 3
- 230000002787 reinforcement Effects 0.000 claims description 3
- 241000588724 Escherichia coli Species 0.000 abstract description 2
- 244000005700 microbiome Species 0.000 abstract description 2
- 230000000052 comparative effect Effects 0.000 description 13
- 230000005486 microgravity Effects 0.000 description 7
- 230000001681 protective effect Effects 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
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- 239000000758 substrate Substances 0.000 description 2
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
- FOIXSVOLVBLSDH-UHFFFAOYSA-N Silver ion Chemical compound [Ag+] FOIXSVOLVBLSDH-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000002696 acid base indicator Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003593 chromogenic compound Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
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- 239000000203 mixture Substances 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
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- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- -1 silver ions Chemical class 0.000 description 1
- AKHNMLFCWUSKQB-UHFFFAOYSA-L sodium thiosulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=S AKHNMLFCWUSKQB-UHFFFAOYSA-L 0.000 description 1
- 235000019345 sodium thiosulphate Nutrition 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
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- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/10—Enterobacteria
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- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
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Abstract
The invention relates to the technical field of microorganism detection, in particular to an escherichia coli group detection device and a detection method thereof; the coliform detection device comprises a water taking bag, a water taking bag joint assembly and a detection bag, wherein the water taking bag joint assembly comprises a guide pipe, and further comprises a water taking bag joint and a Luer male joint which are respectively arranged at two ends of the guide pipe; pre-filling MMO-MUG dry powder culture medium, LECC dry powder culture medium or lactose peptone dry powder culture medium as detection medium, and culturing for 18-28 hr to accurately detect coliform.
Description
Technical Field
The invention belongs to the technical field of microorganism detection, and particularly relates to an escherichia coli group detection device and a detection method thereof.
Background
In a microgravity environment, pathogenicity of coliform is enhanced, and diseases are more likely to occur (Chin J Aerospace Med, june 2010, vol.21, no.2, p 144-147), so that detection and prevention of coliform in time are very important.
In 2016, NASA used a hand-held mini sequencer to detect coliform bacteria in a sample to be tested by collecting and culturing the sample for at least one week of test time, however, this method is complicated to operate and takes a long time.
The detection method commonly used for coliform is an enzyme substrate method and a multitube fermentation method, wherein the enzyme substrate method is characterized in that coliform grows in a chromogenic medium to generate enzyme, chromogenic substrates in the chromogenic medium are decomposed to release chromogenes so as to change the color of the medium, the detection method is convenient and quick, the false positive is low, a large number of samples are suitable for rapid detection, the commonly used chromogenic medium comprises LECC culture medium and commercial MMO-MUG culture medium, the detection process is simple and rapid, and the detection result is accurate; the multitube fermentation method is a detection method for enabling the acid-base indicator in the culture medium to change in color according to the characteristics of the coliform group bacteria capable of fermenting lactose to produce acid and gas; the detection methods can simply and rapidly detect the coliform group, however, in the microgravity unclean environment, the sample collection, transfer and culture in the detection process inevitably generate open operation, so that pollution is caused, the detection result is inaccurate, and therefore, a device suitable for the environment needs to be provided to rapidly and accurately detect the coliform group.
The prior art has little research on the coliform detection device under the microgravity unclean environment, and particularly the coliform detection device which prevents pollution in the detection process, is simple to operate and has accurate results is more difficult to find.
Disclosure of Invention
Therefore, the technical problem to be solved by the invention is to overcome the technical problems of inaccurate results caused by complex operation and easy pollution of coliform detection in a microgravity unclean state in the prior art, and provide the coliform detection device and the detection method for transferring samples to a detection bag in a fully closed state and mixing the samples with a culture medium for culture in the microgravity unclean state, so that the operation is simple and quick, and pollution is avoided, thereby obtaining accurate detection results.
The invention provides a coliform group detection device, which comprises the following components,
The detection bag comprises a detection bag body and a Luer female connector arranged at one end of the detection bag body, wherein a detection cavity suitable for storing detection media is formed in the detection bag body, and a detection bag one-way valve allowing a sample to flow into the detection bag is connected to the Luer female connector;
The water taking bag connector assembly comprises a guide pipe, and further comprises a water taking bag connector and a Luer male connector which are respectively arranged at two ends of the guide pipe;
The water taking bag comprises a water taking bag body and a water taking bag interface arranged on the water taking bag body, and a silica gel valve with elasticity is fixed at the water taking bag interface in a sealing way
When the coliform group detection device is assembled, the water taking bag connector is connected with the silica gel valve in a matched mode, and the Luer male connector is connected with the detection bag one-way valve in a matched mode.
Preferably, the detection medium is a chromogenic dry powder culture medium, the volume of the detection bag body is 100-500ml, and the volume ratio of the weight of the detection medium to the volume of the detection bag body is (2.3 g-2.8 g): 100ml.
Preferably, the chromogenic dry powder culture medium is selected from one of an MMO-MUG dry powder culture medium, a LECC dry powder culture medium or a lactose peptone dry powder culture medium, and is preloaded into a detection cavity of the detection bag body when the detection bag is prepared.
Preferably, the detection bag further comprises a Luer male cap plug connected to the Luer female connector through a protection rope, and the Luer male cap plug is connected with the detection bag one-way valve to seal and protect the detection bag before the coliform detection device is assembled.
Preferably, the water taking bag connector and the Luer male connector are connected with the inner diameters of the two ends of the guide pipe respectively through interference fit and auxiliary glue reinforcement, and the interference is more than or equal to 0.6mm.
Preferably, the water intake bag connector assembly further comprises a water intake bag connector protecting sleeve and a Luer female cap plug, the water intake bag connector protecting sleeve is connected with the water intake bag connector assembly through protecting ropes respectively, the water intake bag connector protecting sleeve seals and protects the water intake bag connector before the coliform bacteria group detection device is assembled, and the Luer female cap plug seals and protects the Luer male connector.
Preferably, the check valve of the check bag is in sealing connection with the male Luer connector, the check valve of the check bag is in sealing connection with the male Luer cap plug, the male Luer connector is in sealing connection with the female Luer cap plug, and the check valve of the check bag is in threaded positioning in a matching manner.
Preferably, the Luer female connector and the check valve of the detection bag adopt a double fixing mode of thread locking and glue bonding.
The invention also provides a method for detecting coliform by using the coliform detection device, which comprises the following steps,
S1, injecting a water sample to be detected into a water taking bag through a silica gel valve;
S2, unscrewing a Luer female cap plug on a Luer male connector of the water taking bag connector assembly, unscrewing a Luer male cap plug on a check valve of the detection bag, and connecting the check valve of the detection bag with the Luer male connector;
S3, taking the water taking bag connector protective sleeve, inserting the water taking bag connector into a water taking bag containing a water sample to be measured through a silica gel valve, transferring the water sample to be measured in the water taking bag into a detection bag through extrusion, after the water sample is transferred, pulling out the water taking bag connector, screwing off a Luer male connector, and sealing the opening by using respective cap plugs;
s4, shaking the detection bag containing the water sample to enable the detection medium in the detection bag to be completely dissolved, culturing the detection bag at 37 ℃ for 18-28h, and obtaining a detection result of coliform by observing the color change of the culture.
The technical scheme of the invention has the following beneficial effects:
1. in order to realize carrying out coliform detection to the water sample under microgravity environment, use water intaking bag joint assembly sealing connection water intaking bag and detect the bag, during the detection, water intaking bag joint of water intaking bag joint assembly one end cooperates sealing connection with the silica gel valve of water intaking bag interface department, the Luer male joint of water intaking bag joint assembly other end and the detection bag check valve sealing connection on the detection bag, shift the water sample from the water intaking bag to detect the bag under totally closed state and cultivate after detecting the medium and mix and carry out the detection of coliform, easy operation has avoided the pollution that probably takes place in the testing process, can obtain accurate testing result.
2. The water taking bag joint of water taking bag joint assembly one end adopts interference fit and assists glue to consolidate with the pipe junction, utilizes the elasticity of pipe to realize sealing connection, and the water taking bag joint is according to the silica gel valve size design of water taking bag interface department, inserts sealing connection in the silica gel valve with the water taking bag joint during the detection, is equipped with water taking bag joint protective sheath when not assembling, prevents to be polluted before the assembly, guarantees the accuracy of testing result.
3. The Luer male connector at the other end of the water taking bag connector assembly is in interference fit with the connecting end of the guide pipe and is reinforced by glue, separation is avoided, so that the separation risk of products is reduced, sealing is ensured to be reliable, the connecting position of the Luer male connector and the check valve of the detection bag during detection, and the connecting position of the Luer female cap plug and the Luer male connector, which are arranged before assembly, are in sealing connection through Luer taper, and screw thread positioning is matched, so that connection is sealed and stable.
4. The Luer female connector of the detection bag and one end connected with the detection bag body are in combined action through interference fit and glue adhesion, leakage is avoided, the joint of the other end and the check valve of the detection bag adopts a double fixing mode of locking with the glue by self threads, leak-free sealing connection is achieved, the connecting end of the check valve of the detection bag and the connector assembly of the water taking bag is further provided with a Luer male cap plug to seal before assembly, sealing protection is carried out on a detection medium pre-installed before detection and a culture system injected with a water sample, pollution is prevented, and therefore accurate detection results are obtained.
5. The protecting ropes are arranged on the Luer male cap plug, the Luer female cap plug and the water taking bag connector protecting sleeve, so that the parts cannot be scattered and lost in a microgravity environment.
6. The MMO-MUG dry powder culture medium, LECC dry powder culture medium and lactose peptone dry powder culture medium are ideal culture mediums for detecting coliforms, the culture medium is easy to obtain, the detection accuracy rate of the coliforms is high, the detection period is short, and the practicability is good.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present invention, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram showing the structure of a coliform detection device before assembly in the present embodiment;
FIG. 2 is a schematic diagram showing the assembled structure of the coliform detection device according to the present embodiment;
fig. 3 is a schematic view of the structure of the area a in fig. 1.
FIG. 4 is a schematic view of a Luer female connector according to the present embodiment
Reference numerals illustrate:
1. A detection bag; 11. detecting the bag body; 12. a Luer female connector; 121. a connection protrusion; 13. detecting a check valve of the bag; 14. luer male cap plug; 2. a water taking bag joint assembly; 21. a water intake bag joint; 22. a conduit; 23. a Luer male connector; 24. the water taking bag is connected with the protective sleeve; 25. luer female cap plugs; 3. a water taking bag; 31. a water taking bag body; 32. a water taking bag interface; 33. a silicone valve; 4. and (5) protecting the rope.
Detailed Description
The following description of the embodiments of the present invention will be made apparent and fully in view of the accompanying drawings, in which some, but not all embodiments of the invention are shown. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In addition, the technical features of the different embodiments of the present invention described below may be combined with each other as long as they do not collide with each other.
Example 1
As shown in fig. 1-4, the present embodiment provides an apparatus for detecting coliform bacteria, comprising,
The detection bag 1 comprises a detection bag body 11 and a Luer female connector 12 arranged at one end of the detection bag body 11, wherein the Luer female connector 12 is connected with a detection bag one-way valve 13 allowing a sample to flow into the detection bag 1, the detection bag body 11 is made of PVC composite material, has stronger chemical stability, elastic modulus and flexibility, the detection bag 1 is used as a detection cavity suitable for sealing detection media, mixing the detection media and the sample to be detected and a container for culturing and observing and storing culture, the edge of the detection bag body 11 adopts fusion sealing, the whole can ensure that the internal detection media cannot leak for more than two years, the volume is designed to be 100ml, a water sample to be detected is directly filled during use, and the measurement of the filling liquid amount is not needed, wherein the detection media is a chromogenic dry powder culture medium MMO-MUG;
the detection medium of the embodiment is arranged in the detection bag body 11 in advance, and the manufacturing process comprises the steps of after EO sterilization (ethylene oxide sterilization) of the detection bag, subpackaging an MMO-MUG dry powder culture medium into the sterile detection bag 1 under a clean environment with the relative humidity less than or equal to 50%, flattening the bag body, adhering the unthreaded end of the Luer female connector 12, connecting one end of the Luer female connector 12 connected with the detection bag body 11 through interference fit and the joint effect of glue, avoiding leakage, and arranging a connecting protrusion 121 for preventing falling at the connecting position, wherein the weight of the MMO-MUG dry powder culture medium is 2.7g.
The water taking bag connector assembly 2 comprises a guide pipe 22, and further comprises a water taking bag connector 21 and a Luer male connector 23 which are respectively arranged at two ends of the guide pipe 22, wherein the water taking bag connector assembly 2 is connected with the water taking bag 3 and the detection bag 1 to provide a totally-enclosed sample transferring channel;
the water taking bag 3 comprises a water taking bag body 31 and a water taking bag interface 32 arranged on the water taking bag body 31, a silica gel valve 33 with elasticity is fixed at the water taking bag interface 32 in a sealing way, the water sample to be detected is injected into the water taking bag 3 in the front direction during detection, a power device is not needed during detection, and the water sample to be detected can be extruded into the detection bag 1 in a fully-closed state through manual extrusion;
because the trace silver ions contained in the water sample to be detected can influence the detection rate of coliform groups, the water taking bag 3 is added with a silver ion neutralizer sodium thiosulfate;
When the coliform group detection device is assembled, the water taking bag connector 21 is matched and connected with the silica gel valve 33, and the Luer male connector 23 is matched and connected with the detection bag one-way valve 13.
The silica gel valve 33 has elasticity, so that the joint of the water intake bag joint 21 and the silica gel valve 33 is completely closed, and leakage and pollution are prevented; the check valve 13 of the test bag, when in place, provides a leak-free connection to the Luer female connector 12, preventing dry powder or liquid from escaping outwardly from the bag during storage and culture, while allowing the water sample to be tested to flow into the test bag 1.
The detection bag 1 further comprises a Luer male cap plug 14 connected to the Luer female connector 12 through a protection rope 4, and the Luer male cap plug is connected with the detection bag one-way valve 13 before the coliform detection device is assembled, so that the detection bag 1 is sealed and protected.
The conduit 22 has elasticity, the conduit 22 is made of high-elasticity transparent PVC material, has higher transparency and elasticity, and has the function of connecting the water taking bag connector 21 and the Luer male connector 23, wherein the water taking bag connector 21 and the Luer male connector 23 are respectively connected with the inner diameters of the two ends of the conduit 22 in an interference fit and assisted with glue reinforcement mode, the interference is 0.6mm, and the interference is not less than 0.6mm when other embodiments are carried out.
The Luer male connector 23 and the connecting end of the guide pipe 22 are matched in a taper manner, separation is avoided through right-angle closing, the water intake bag connector 21 is designed according to the size of a silica gel valve 33 at the water intake bag connector 32, the water intake bag connector assembly 2 further comprises a water intake bag connector protecting sleeve 24 and a Luer female cap plug 25, the water intake bag connector protecting sleeve 24 is connected with the water intake bag connector assembly 2 through protecting ropes 4 respectively, before the coliform bacteria detection device is assembled, the water intake bag connector 21 is sealed and protected by the water intake bag connector protecting sleeve 24, the risk of exogenous pollution caused by unnecessary contact is avoided, the Luer female cap plug 25 is connected with the Luer male connector 23 in a sealing manner, and pollution before the assembly is avoided after products are taken out from packaging bags.
The Luer male connector 23 is connected with the detection bag check valve 13, the Luer male connector 23 is connected with the Luer female cap plug 25, and the detection bag check valve 13 is connected with the Luer male cap plug 14 in a sealing manner through Luer taper and is matched with threaded positioning.
The Luer female connector 12 and the check valve 13 of the detection bag adopt a double fixing mode of thread locking and glue bonding.
Example 2
This example provides an apparatus for detecting coliform bacteria wherein the volume of the detection bag is 200ml, and the detection bag is preloaded with 5.6g of LECC dry powder medium, the remainder of this example being as described in example 1.
Example 3
This example provides an apparatus for detecting coliform bacteria wherein the volume of the detection bag is 500ml, and the detection bag is preloaded with 11.5g of lactose peptone dry powder medium, the remainder of this example being as described in example 1.
Example 4
The present embodiment provides a method for detecting coliform bacteria by using the coliform bacteria detection device of embodiment 1, which specifically comprises the following steps:
s1, injecting a water sample A to be detected into a water taking bag 3 through a silica gel valve 33, and shaking the water taking bag 3;
S2, unscrewing a Luer female cap plug 25 of the water taking bag connector assembly 2, unscrewing a Luer male cap plug 14 on the detection bag 1, and connecting the detection bag one-way valve 13 with a Luer male connector 23 of the water taking bag connector assembly 2;
s3, taking the water taking bag connector protective sleeve 24 out, inserting the water taking bag connector 21 into the water taking bag 3 containing the water sample to be measured through the silica gel valve 33, transferring the water sample to be measured in the water taking bag 3 into the detection bag 1 through extrusion, after the water sample is transferred, pulling out the water taking bag connector 21, screwing off the Luer male connector 23, and sealing the opening by using respective cap plugs;
S4, shaking the detection bag 1 containing the water sample, completely dissolving the MMO-MUG dry powder culture medium in the detection bag, culturing at 37 ℃, and recording the color change of the culture medium after culturing for 24 hours and 28 hours respectively.
Example 5
The present embodiment provides a method for detecting coliform bacteria by using the coliform bacteria detection device of embodiment 2, which specifically comprises the following steps:
S1, injecting a water sample B to be detected into a water taking bag 3 through a silica gel valve 33, and shaking the water taking bag 3;
S2, unscrewing a Luer female cap plug 25 of the water taking bag connector assembly 2, unscrewing a Luer male cap plug 14 on the detection bag 1, and connecting the detection bag one-way valve 13 with a Luer male connector 23 of the water taking bag connector assembly 2;
s3, taking the water taking bag connector protective sleeve 24 out, inserting the water taking bag connector 21 into the water taking bag 3 containing the water sample to be measured through the silica gel valve 33, transferring the water sample to be measured in the water taking bag 3 into the detection bag 1 through extrusion, after the water sample is transferred, pulling out the water taking bag connector 21, screwing off the Luer male connector 23, and sealing the opening by using respective cap plugs;
S4, shaking the detection bag 1 containing the water sample, dissolving the LECC dry powder culture medium in the detection bag completely, culturing the detection bag at 37 ℃, and recording the color change of the culture medium for 18h and 24h respectively.
Example 6
The present embodiment provides a method for detecting coliform bacteria by using the coliform bacteria detection device of embodiment 3, which specifically comprises the following steps:
s1, injecting a water sample C to be detected into a water taking bag 3 through a silica gel valve 33, and shaking the water taking bag 3;
S2, unscrewing a Luer female cap plug 25 of the water taking bag connector assembly 2, unscrewing a Luer male cap plug 14 on the detection bag 1, and connecting the detection bag one-way valve 13 with a Luer male connector 23 of the water taking bag connector assembly 2;
s3, taking the water taking bag connector protective sleeve 24 out, inserting the water taking bag connector 21 into the water taking bag 3 containing the water sample to be measured through the silica gel valve 33, transferring the water sample to be measured in the water taking bag 3 into the detection bag 1 through extrusion, after the water sample is transferred, pulling out the water taking bag connector 21, screwing off the Luer male connector 23, and sealing the opening by using respective cap plugs;
S4, shaking the detection bag 1 containing the water sample, dissolving all lactose peptone dry powder culture medium in the detection bag, culturing at 37 ℃, and recording the color change of the culture medium after culturing for 22 hours and 26 hours respectively.
Comparative example 1
The comparative example provides a method for detecting coliform bacteria by using the coliform bacteria detection device described in the embodiment 1, which is specifically as follows:
the same procedure as in example 4 was used for monitoring and observation using sterile pure water as the water sample D to be measured.
Comparative example 2
The comparative example provides a method for detecting coliform bacteria by using the coliform bacteria detection device described in the embodiment 2, which is specifically as follows:
the same procedure as in example 5 was used for monitoring and observation using sterile pure water as the water sample E to be measured.
Comparative example 3
The comparative example provides a method for detecting coliform bacteria by using the coliform bacteria detection device described in the embodiment 3, which is specifically as follows:
the same procedure as in example 6 was used for monitoring and observation using sterile pure water as the water sample F to be measured.
Detection result
The results of the culture of examples 4-6 and comparative examples 1-3 are shown in tables 1-3 below.
Table 1: example 4 and comparative example 1 culture results schematic Table
| Cultivation time (h) | Example 4 | Comparative example 1 |
| 24h | Yellow colour | Does not change color |
| 28h | Yellow colour | Does not change color |
Table 2: example 5 and comparative example 2 culture results schematic Table
| Cultivation time (h) | Example 5 | Comparative example 2 |
| 18h | Blue color | Does not change color |
| 24h | Blue color | Does not change color |
Table 3: table showing results of culturing in example 6 and comparative example 3
| Cultivation time (h) | Example 6 | Comparative example 3 |
| 22h | Yellow colour | Does not change color |
| 26h | Yellow colour | Does not change color |
As described above, the detection result can be obtained by using the coliform detection device and collecting the sample containing coliform for culturing for 18-28 hours, the detection period is short, the operation is convenient, and the detection method can effectively avoid pollution and has good accuracy.
It is apparent that the above examples are given by way of illustration only and are not limiting of the embodiments. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. While still being apparent from variations or modifications that may be made by those skilled in the art are within the scope of the invention.
Claims (1)
1. A method for detecting coliform using a coliform detection device, characterized in that the coliform detection device comprises the following components,
The detection bag (1), the detection bag (1) comprises a detection bag body (11) and a Luer female connector (12) arranged at one end of the detection bag body (11), a detection cavity suitable for storing detection media is arranged in the detection bag body (11), and a detection bag one-way valve (13) allowing samples to flow into the detection bag (1) is connected to the Luer female connector (12);
the water taking bag joint assembly (2) comprises a guide pipe (22), and further comprises a water taking bag joint (21) and a Luer male joint (23) which are respectively arranged at two ends of the guide pipe (22);
the water taking bag (3) comprises a water taking bag body (31) and a water taking bag interface (32) arranged on the water taking bag body (31), and an elastic silica gel valve (33) is fixed at the water taking bag interface (32) in a sealing way;
When the coliform detection device is assembled, the water taking bag connector (21) is connected with the silica gel valve (33) in a matched mode, and the Luer male connector (23) is connected with the detection bag one-way valve (13) in a matched mode;
The detection medium is a chromogenic dry powder culture medium, the volume of the detection bag body (11) is 100-500ml, and the volume ratio of the weight of the detection medium to the volume of the detection bag body (11) is (2.3 g-2.8 g): 100ml;
The color development dry powder culture medium is selected from one of an MMO-MUG dry powder culture medium, LECC dry powder culture medium or lactose peptone dry powder culture medium, and is preloaded into a detection cavity of the detection bag body (11) when the detection bag (1) is prepared;
The detection bag (1) further comprises a Luer male cap plug (14) connected to the Luer female connector (12) through a protection rope (4), and before the coliform detection device is assembled, the Luer male cap plug (14) is connected with the detection bag one-way valve (13) to carry out sealing protection on the detection bag (1);
the water taking bag connector (21) and the Luer male connector (23) are respectively connected with the inner diameters of the two ends of the guide pipe (22) in an interference fit and assisted by glue reinforcement mode, and the interference is more than or equal to 0.6mm;
The water taking bag joint assembly (2) further comprises a water taking bag joint protection sleeve (24) and a Luer female cap plug (25), the water taking bag joint protection sleeve (24) is connected with the water taking bag joint assembly (2) through a protection rope (4), before the coliform bacteria detection device is assembled, the water taking bag joint protection sleeve (24) seals and protects the water taking bag joint (21), and the Luer female cap plug (25) seals and protects the Luer male joint (23);
the detection bag one-way valve (13) is connected with the Luer male plug (23), the detection bag one-way valve (13) is connected with the Luer male cap plug (14), the Luer male plug (23) is connected with the Luer female cap plug (25) in a sealing way through Luer taper and is matched with screw thread positioning;
the Luer female connector (12) and the check valve (13) of the detection bag adopt a double fixing mode of thread locking and glue bonding;
The method for detecting coliform group bacteria comprises the following steps,
S1, injecting a water sample to be detected into a water taking bag (3) through a silica gel valve (33);
S2, unscrewing a Luer female cap plug (25) on a Luer male connector (23) of the water taking bag connector assembly (2), unscrewing a Luer male cap plug (14) on a check valve (13) of the check bag (1), and connecting the check valve (13) of the check bag with the Luer male connector (23);
S3, taking down the water taking bag joint protection sleeve (24), inserting the water taking bag joint (21) into the water taking bag (3) containing the water sample to be tested through the silica gel valve (33), transferring the water sample to be tested in the water taking bag (3) into the detection bag (1) through extrusion, after the water sample is transferred, pulling out the water taking bag joint (21), screwing off the Luer male joint (23), and closing the opening by using respective cap plugs;
S4, shaking the detection bag (1) containing the water sample, dissolving all detection media in the detection bag, culturing the detection bag for 18 to 28 hours at 37 ℃, and obtaining the detection result of coliform bacteria by observing the color change of the culture medium.
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