CN209327359U - Newcastle disease quick detection kit based on ELISA - Google Patents

Abstract

The newcastle disease quick detection kit based on ELISA that the utility model proposes a kind of, including pre-coated plate, bracket and drain picker, the pre-coated plate includes backboard and upper cover, the upper lid is equipped with 96 loading slots, and the upper cover is fastened on backboard, and the backboard two sides are detachably equipped with bracket, supporting table is equipped with above the bracket, groove is equipped in the middle part of the supporting table, the both sides of the edge of the drain picker are inserted into groove, and the liquid outlet of the drain picker connects syringe.When needing to clean or change liquid, it is only necessary to backboard two sides be installed bracket, and drain picker is mounted in supporting table, entire drain picker is realized by syringe under manual control, and washing lotion and drain facilitate portable, it occupies little space, can be manually adjusted at any time, it is simple and practical.

Description

Newcastle disease quick detection kit based on ELISA

Technical field

The utility model relates to live vaccine antibody test technical field, in particular to a kind of newcastle disease based on ELISA Quick detection kit.

Background technique

Newcastle disease virus is a kind of acute, hot, septic of one kind and highly contagious disease that can cause fowl.It is main It wants lesion to show as having a fever, has difficulty in breathing, polarity dysentery, neurological disorders, the features such as mucous membrane and serous coat bleeding.Generally passed in birds It broadcasts, velogen strain can make this population extinction, and low virulent strain can cause fowl upper infection and egg production decline, but can be quickly Restore, while the mankind can be infected and cause conjunctivitis or adenolymphitis, but just rehabilitation quickly.

Newcastle disease C2 vaccine Inoculation antienzyme linked immunosorbent assay (Enzyme-Linked ImmunoSorbent Assay, ELISA) detection kit, be using based on live Newcastle disease vaccine, using ELISA detection technique, preparation it is quick Detection kit.The inspection testing cassete can be to the birds after 15 days live Newcastle disease vaccines of inoculation or inactivated vaccine, by acquiring fowl The blood or serum of class, excreta etc. detect internal vaccine inoculation situation, if generate newcastle epidemic disease antibody, and carry out semidefinite Measure examination.

Existing ELISA is integrated large scale equipment, inconvenient to carry although high degree of automation, simultaneously because It when ELISA method is detected, needs repeatedly to wash and dilute, repeatedly check and correction is needed using the equipment, it is cumbersome.

Utility model content

The purpose of this utility model aims to solve at least one of the technological deficiency.

To achieve the goals above, it is fast to provide a kind of newcastle disease based on ELISA for the embodiment of the utility model one side Fast detection kit, including pre-coated plate, bracket and drain picker, the pre-coated plate include backboard and upper cover, it is described on It covers and is equipped with 96 loading slots, the upper cover is fastened on backboard, and the backboard two sides are detachably equipped with bracket, on the bracket Side is equipped with supporting table, and groove is equipped in the middle part of the supporting table, and the both sides of the edge of the drain picker are inserted into groove, the row The liquid outlet of liquid picker connects syringe.

Preferably, the bracket is equipped with scale.

In any of the above-described scheme preferably, the supporting table is fixed by snap on bracket.

In any of the above-described scheme preferably, the drain picker includes that plate and be fixedly connected with bottom plate 96 take Fluid column, each outlet bottom plate for taking fluid column, the bottom plate is hollow structure, and the bottom plate side is equipped with liquid outlet.

In any of the above-described scheme preferably, the backboard is equipped with boss, and the boss is for fixing loading slot.

In any of the above-described scheme preferably, the supporting table is integrally fixed on bracket, and the bracket is telescopic rod.

In any of the above-described scheme preferably, the bracket is equipped with sliding rail, and the supporting table is along sliding rail Sliding, the sliding rail are equipped with brake block.

In any of the above-described scheme preferably, multiple threaded holes are equipped in the middle part of the sliding rail, in the threaded hole Equipped with threaded post, brake block is fixed in the threaded post, the brake block is rotated around threaded post.

According to a kind of newcastle disease quick detection kit based on ELISA provided by the embodiment of the utility model, compared to Currently available vaccines, existing vaccines antibody assay kit has at least the following advantages: when being detected due to ELISA method, need repeatedly wash and Dilution, therefore by the way that bracket is removably arranged on backboard, when needing to clean or change liquid, it is only necessary to which backboard two sides are installed Good bracket, and drain picker is mounted in supporting table, entire drain picker is realized by syringe under manual control, is washed Liquid and drain, it is convenient, it occupies little space, while easy to operate, when for cleaning or change liquid, required height is asynchronous, can It is simple and practical to be manually adjusted at any time.

The additional aspect of the utility model and advantage will be set forth in part in the description, partially will be from following description In become obvious, or recognized by the practice of the utility model.

Detailed description of the invention

The above-mentioned and/or additional aspect and advantage of the utility model from the description of the embodiment in conjunction with the following figures will Become obvious and be readily appreciated that, in which:

Fig. 1 is a kind of vertical view of the newcastle disease quick detection kit based on ELISA provided by the embodiment of the utility model Schematic diagram；

Fig. 2 is that a kind of newcastle disease quick detection kit test based on ELISA provided by the embodiment of the utility model is shown It is intended to

Fig. 3 is a kind of drain of the newcastle disease quick detection kit based on ELISA provided by the embodiment of the utility model Picker schematic diagram；

Fig. 4 be a kind of newcastle disease quick detection kit bracket based on ELISA provided by the embodiment of the utility model and The connected mode schematic diagram of supporting table；

In figure:

1, pre-coated plate；2, bracket；3, drain picker；101, backboard；102, upper cover；4, supporting table；401, groove； 301, liquid outlet；5, syringe；201, brake block；202, sliding rail；203, threaded hole；

Specific embodiment

The embodiments of the present invention are described below in detail, examples of the embodiments are shown in the accompanying drawings, wherein from beginning Same or similar element or element with the same or similar functions are indicated to same or similar label eventually.Below by ginseng The embodiment for examining attached drawing description is exemplary, it is intended to for explaining the utility model, and should not be understood as to the utility model Limitation.

As shown in Figs. 1-2, a kind of newcastle disease quick detection kit based on ELISA of the utility model embodiment, packet Pre-coated plate 1, bracket 2 and drain picker 3 are included, the pre-coated plate 1 includes backboard 101 and upper cover 102, the upper cover 102 96 loading slots are equipped with, the upper cover 102 is fastened on backboard 101, and 101 two sides of backboard are detachably equipped with bracket 2, institute It states and is equipped with supporting table 4 above bracket 2, groove 401 is equipped in the middle part of the supporting table 4, the both sides of the edge of the drain picker 3 are inserted Enter in groove 401, the liquid outlet 301 of the drain picker 3 connects syringe 5.The backboard is equipped with boss, the boss For fixing loading slot.

As shown in figure 3, the bracket 2 is equipped with scale.The supporting table 4 is fixed by snap on bracket 2.The row Liquid picker 3 includes that plate and be fixedly connected with bottom plate 96 take fluid column, each outlet bottom plate for taking fluid column, described Bottom plate is hollow structure, and the bottom plate side is equipped with liquid outlet 301, and liquid outlet connects syringe, manual withdrawal or moves back pushing Syringe realizes imbibition and drain.

Preferably, the supporting table 4 is integrally fixed on bracket 2, and the bracket 2 is telescopic rod.In the present embodiment, will Bracket 2 is set as telescopic rod, and telescopic rod can use common multi-joint telescoping structure, by adjusting the length of telescopic rod, realizes The adjusting of supporting table height.

As shown in figure 4, the bracket 2 is equipped with sliding rail, the supporting table 4 is slided along sliding rail, the bracket 2 It is equipped with sliding rail 202, the supporting table 4 is slided along sliding rail 202, and specially supporting table is equipped with fluting, edge of slotting Sliding rail sliding, the sliding rail 202 be equipped with brake block 201.Further, it is equipped in the middle part of the sliding rail 202 Multiple threaded holes 203 are equipped with threaded post in the threaded hole 203, are fixed with brake block 201, the system in the threaded post Motion block 201 is rotated around threaded post.The present embodiment is the fixed brake block in one end using threaded post, and the other end screws in threaded hole, When supporting table is slided along sliding rail, behind the position to be fixed of determination, threaded post is screwed in the threaded hole of the position, with back spin Transformation of ownership motion block, supporting table is fixed in the position.It should be noted that brake block width and sliding rail width always, The length of brake block is greater than the width of bracket, guarantees barrier support platform after brake block transverse rotation.

When in use, newcastle disease vaccine lyophilized protein is diluted to 5ug or so with PH9.6 carbonate buffer solution, uses 96 The pre-coated plate (upper cover) in hole, the newcastle disease vaccine lyophilized protein in upper cover after each Kong Zhongjia 0.1ml dilution, upper cover and backboard It fastens, 4 DEG C overnight, later mounting bracket and supporting table, and the groove among supporting table installs drain picker, at this time drain Picker is emptying state, by extracting syringe, discards the solution in the pre-coated plate in 96 holes, then drain picker enters liquid Mouth is placed in cleaning solution, is cleaned 3 times using syringe plus cleaning solution, 3 minutes every time, is completed cleaning.

After the completion of cleaning, drain picker 3 is removed, sample is added to the coated good hole of 96 orifice plates one by one In, then drain picker 3 is installed again, 37 DEG C incubation 1-2 hours, it is rear by extracting syringe, discard the pre-coated plate in 96 holes In solution, then the liquid inlet of drain picker is placed in cleaning solution, and syringe plus cleaning solution is utilized to clean 3 times, every time 3 points Clock completes cleaning.Using deionization ultrapure water as blank control, and positive reference substance is diluted to different concentration and is added Into coating hole, using different concentration, standard detection line is established.

Then, by extracting syringe, it is about 100ul that enzyme labelled antibody is added in each well, and 37 DEG C of incubations 1 are small When, then the liquid inlet of drain picker is placed in cleaning solution, is cleaned 3 times using syringe plus cleaning solution, 3 minutes every time, complete At cleaning.

Display substrate TMB chromogenic substrate 100ul is added in every hole using drain picker, 37 DEG C are incubated for 30 minutes.

Every hole is added 1mol sulfuric acid solution and carries out termination reaction.

96 orifice plates are placed on ELISA detecting instrument, are detected using OD450mm, are zeroing with blank control.It is logical Positive control is crossed, standard curve is set up.By the detected value reference standard curve of sample, antibody content in sample is calculated.By This can be to birds after being inoculated with newcastle disease vaccine, and the antibody generated to vaccine is assessed, and can effectively detect the effective of vaccine Birds are prevented newcastle disease from playing the role of monitoring by property and effect of inoculation.

In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show The description of example " or " some examples " etc. means specific features, structure, material or spy described in conjunction with this embodiment or example Point is contained at least one embodiment or example of the utility model.In the present specification, to the schematic table of above-mentioned term Stating may not refer to the same embodiment or example.Moreover, particular features, structures, materials, or characteristics described can be It can be combined in any suitable manner in any one or more embodiment or examples.

Although the embodiments of the present invention have been shown and described above, it is to be understood that above-described embodiment is Illustratively, it should not be understood as limiting the present invention, those skilled in the art are not departing from the utility model Principle and objective in the case where above-described embodiment can be changed in the scope of the utility model, modify, replace and Modification.The scope of the utility model is by appended claims and its equivalent limits.

Claims (8)

1. a kind of newcastle disease quick detection kit based on ELISA, including pre-coated plate, bracket and drain picker, special Sign is that the pre-coated plate includes backboard and upper cover, and the upper lid is equipped with 96 loading slots, and the upper cover is fastened on backboard On, the backboard two sides are detachably equipped with bracket, and it is equipped with supporting table above the bracket, is equipped with groove in the middle part of the supporting table, In the both sides of the edge insertion groove of the drain picker, the liquid outlet of the drain picker connects syringe.

2. the newcastle disease quick detection kit according to claim 1 based on ELISA, which is characterized in that the bracket It is equipped with scale.

3. the newcastle disease quick detection kit according to claim 2 based on ELISA, which is characterized in that the support Platform is fixed by snap on bracket.

4. the newcastle disease quick detection kit according to claim 1 based on ELISA, which is characterized in that the drain Picker includes that plate and be fixedly connected with bottom plate 96 take fluid column, each outlet bottom plate for taking fluid column, the bottom Plate is hollow structure, and the bottom plate side is equipped with liquid outlet.

5. the newcastle disease quick detection kit according to claim 1 based on ELISA, which is characterized in that the backboard It is equipped with boss, the boss is for fixing loading slot.

6. the newcastle disease quick detection kit according to claim 2 based on ELISA, which is characterized in that the support Platform is integrally fixed on bracket, and the bracket is telescopic rod.

7. the newcastle disease quick detection kit according to claim 2 based on ELISA, which is characterized in that the bracket It is equipped with sliding rail, the supporting table is slided along sliding rail, and the sliding rail is equipped with brake block.

8. the newcastle disease quick detection kit according to claim 7 based on ELISA, which is characterized in that the sliding It is equipped with multiple threaded holes in the middle part of track, threaded post is equipped in the threaded hole, is fixed with brake block in the threaded post, it is described Brake block is rotated around threaded post.