CN209260092U - The automatic training station of parallel cell - Google Patents

Abstract

The utility model belongs to biological culture apparatus field, it is related to a kind of automatic training station of parallel cell, including working station frame, the lower plate upper surface of working station frame is set there are three region, respectively parallel cell cultivation region, vessel rest area and culture solution holding vessel rest area, working station frame upper end are equipped with the liquid relief sampler with these three region cooperatings；A number of parallel cell culture apparatus is equipped in parallel cell cultivation region.The structure design of the utility model is reasonable, can complete big throughput cell culture, and cell culture operations are convenient, and device structure modularization, and structure is relatively clear, mount and dismount more convenient.

Description

The automatic training station of parallel cell

Technical field

The utility model relates to a kind of automatic training stations of parallel cell, belong to biological culture apparatus field.

Background technique

Parallel cell culture systems are one, and that microorganism, human archeocyte, animal and plant cells are carried out under controlled condition is parallel The system of culture, set of system have several groups fermentation unit, and volume of culture range is 200-1000mL, support more set system connection With to have bigger culture flux.Existing cell cultivation equipment structure and the various complexity of components, installation and removal are not It is convenient.

Utility model content

The purpose of this utility model is that proposing a kind of automatic training station of parallel cell, which cultivates automatically Work station can complete big throughput cell culture, and cell culture operations are convenient, and device structure modularization, structure are more clear It is clear, it mounts and dismounts more convenient.

The automatic training station of parallel cell described in the utility model, including working station frame, working station frame Lower plate upper surface set there are three region, respectively parallel cell cultivation region, vessel rest area and culture solution holding vessel are placed Area, working station frame upper end are equipped with the liquid relief sampler with these three region cooperatings；Parallel cell is set in cultivation region There is a number of parallel cell culture apparatus.

The lower plate upper surface of working station frame is from left to right successively arranged culture solution holding vessel rest area, parallel cell Cultivation region and vessel rest area, are placed with multiple culture solution holding vessels in culture solution holding vessel rest area, in culture solution holding vessel Various reagents needed for cell cultivation process are stored, are mentioned for several parallel cell culture apparatus in parallel cell cultivation region For culture solution；Vessel needed for liquid relief sampler obtains work out of vessel rest area as needed then move to corresponding Work is completed in region, liquid relief sampler is controlled by control system, can be precisely accomplished the work in corresponding region.

Preferably, the liquid relief sampler includes X-axis moving assembly and Y-axis moving assembly, Y-axis moving assembly and X The sliding block of axis moving assembly is fixedly connected, and the sliding block of Y-axis moving assembly is fixedly connected with support transverse slat, and support transverse slat bottom surface is fixed Supporting vertical plate is connected, supporting vertical plate surface is provided with pipette tips moving assembly, gripper moving assembly and sucker moving assembly.X-axis is moved Dynamic component includes horizontally disposed X-axis motor driver and X-axis linear guide, and Y-axis moving assembly includes Y-axis support frame and Y Axis motor driver, the sliding block of X-axis motor driver and the sliding block of X-axis linear guide connect Y-axis support frame, Y-axis support simultaneously Vertical X axis linear guide, Y-axis support frame lower section are equipped with Y-axis motor driver, the cunning of Y-axis motor driver to frame in the horizontal direction Block is fixedly connected with support transverse slat.The movement of X-axis motor driver, drives Y-axis support frame to be moved to specific bit along X-axis linear guide It sets；The movement of Y-axis motor driver drives support transverse slat to be moved to designated position.

Preferably, the gripper moving assembly includes gripper lead screw stepper motor and sliding rail one, gripper lead screw stepping electricity The screw rod of machine is arranged in parallel with sliding rail one, and the sliding block one in the nut and sliding rail one of gripper lead screw stepper motor connects electronic simultaneously Gripper fixed plate, electrical clip grasp fixed board bottom end and are provided with electronic gripper；Gripper lead screw stepper motor rotates forward, and nut drives electronic Gripper fixed plate is moved down along sliding rail one, and electrical clip grasps fixed board and electronic gripper is driven to be moved downward to required crawl position, Then component needed for electronic gripper motion capture, gripper lead screw stepper motor inverts after completing work, and electronic gripper moves up It resets；

Pipette tips moving assembly includes pipette tips lead screw stepper motor and sliding rail two, the screw rod and sliding rail of pipette tips lead screw stepper motor Two are arranged in parallel, and the sliding block two in the nut and sliding rail two of pipette tips lead screw stepper motor connects pipette tips sleeve fixed plate, rifle simultaneously Headgear cylinder is located in pipette tips sleeve fixed plate bottom end by pipette tips sleeve briquetting compression, and pipette tips sleeve top is equipped with threaded sleeve, Threaded sleeve connects 5ml syringe pump, and pipette tips lead screw stepper motor rotates forward, and nut drives pipette tips sleeve fixed plate downward along sliding rail two Mobile, pipette tips sleeve fixed plate drives pipette tips sleeve to be moved to vessel rest area, and pipette tips sleeve obtains shifting out of vessel rest area Liquid rifle, then X-axis moving assembly and the movement of Y-axis moving assembly drive liquid-transfering gun to be moved to sample position, complete sampling work；

Sucker moving assembly includes sucker lead screw stepper motor and sliding rail three, the screw rod and sliding rail of sucker lead screw stepper motor Three are arranged in parallel, the sliding block San Tong method connecting sucker fixed plate in the nut and sliding rail three of sucker lead screw stepper motor, and sucker is solid Fixed board side is equipped with sucker and founds side plate, and sucker founds side plate bottom connecting sucker vacuum box, and sucker vacuum box bottom surface connects vacuum and inhales Disk, sucker vacuum box upper surface connector are connected to the vacuum port of minipump, the exhaust outlet of minipump by corrugated hose Joint noise reduction device；Sucker lead screw stepper motor rotates forward, and nut drives sucker fixed plate to move down along sliding rail San Tong method, and sucker is solid Fixed board drives sucker to found side plate, sucker vacuum box and vacuum chuck and moves down, spiral during sucker vacuum box moves down Hose is stretched, and when vacuum chuck touches the corresponding component in vessel rest area, minipump movement, exhaust outlet leads to It crosses muffler to be vented outwards, vacuum port air-breathing generates vacuum, connects the corrugated hose and sucker vacuum box and true of vacuum port Suction disk is at vacuum, so that corresponding component is sucked vacuum chuck, X-axis moving assembly and the movement of Y-axis moving assembly, cooperation will Vacuum chuck is sucked corresponding component and removes.

Pipette tips lead screw stepper motor, gripper lead screw stepper motor, electronic gripper, sucker lead screw stepper motor, micro vacuum Pump is all connected with control system.Supporting vertical plate bottom is equipped with rotating stepper motor, and rotating stepper motor output shaft bottom end is connected and fixed Motor overturning plate, motor overturning plate upper surface are equipped with fixed sponge, motor overturning plate be located at supporting vertical plate lower section and not with branch Support vertical plate directly contacts, and when rotating stepper motor rotates forward, motor overturning plate is overturn below the liquid-transfering gun carried to pipette tips sleeve, When X-axis motor driver, Y-axis motor driver are mobile, if there is liquid-transfering gun dropping liquid, the drop of drippage can fall in liquid-transfering gun On the sponge of the motor overturning plate upper surface of underface, prevent drips in other regions, when rotating stepper motor reversion, Motor overturning plate is overturn again to supporting vertical plate, is completely offset from immediately below liquid-transfering gun, will not be generated to the decline of liquid-transfering gun Interference effect does not impact the movement of other devices.

Preferably, vessel platform is equipped in the vessel rest area, vessel platform top is equipped with 24 orifice plate areas, pipette tips store area With centrifugation area under control, 24 orifice plate area sides are equipped with 24 orifice plate plastic lid mounting tables, and pipette tips storage area side is moved back equipped with pipette tips unloads component. It is equipped with 24 hole grillage everywhere in 24 orifice plate areas, 24 orifice plates are equipped in 24 hole grillages, is stamped 24 orifice plate plastic lids above 24 orifice plates, 24 Orifice plate area side is equipped with 24 orifice plate plastic lid mounting tables；Pipette tips, which are stored, is equipped with pipette tips support frame in area, place in pipette tips support frame There are several liquid-transfering guns, pipette tips storage area side is moved back equipped with pipette tips unloads component；It is centrifuged in area under control and is equipped with centrifuge tube shelf, in centrifuge tube shelf It is placed with several centrifuge tubes.

Preferably, the parallel cell culture apparatus includes supporting frame, is provided with culture tank above supporting frame and stirs Driving device and parallel bioreactor are mixed, culture tank mixer drive includes driving box body, drives both ends difference in box body Equipped with motor side connecting shaft and tank body side connecting shaft, motor side connecting shaft and tank body side connecting shaft are separately connected stirring motor output Axis and the agitating paddle in parallel cell culture tank.Supporting frame includes Left-side support plate and Right side support plate, Left-side support Plate and Right side support plate are fixedly mounted by positioning pin and main equipment, to culture tank mixer drive and parallel cell culture Tank plays a supportive role.

Preferably, the driving box body includes upper box cover plate and lower casing plate, and upper box cover plate and lower casing board group are at drive Dynamic box case drives the both ends of tray interior to be respectively provided with a synchronous pulley, is connected between two synchronous pulleys by synchronous belt It connects, two synchronous pulley tops are embedded in upper box cover plate top surface, and bearing one is equipped between synchronous pulley and upper box cover plate；Its In be fixed with motor side connecting shaft in the middle part of synchronous pulley, motor side connecting shaft bottom passes through lower casing board bottom plate, passes through The output axis connection of shaft coupling and stirring motor, another synchronous pulley middle part are fixed with tank body side connecting shaft, and tank body side connects Spindle bottom passes through lower casing board bottom plate, connect with agitating paddle in culture tank；Motor side connecting shaft and tank body side connecting shaft Bearing two is equipped between lower casing plate respectively.

When installation, first motor is fixed on supporting frame, parallel cell culture tank is placed on supporting frame, then again Installation driving box body, stirs the motor side connecting shaft for driving box body and tank body side connecting shaft with electric machine main shaft and culture tank respectively Paddle is aligned simultaneously, Fast Installation, completes connection.During driving, electric machine main shaft rotation drives motor side connecting shaft and consolidates with it The synchronous pulley of fixed synchronous pulley rotation, this side drives the synchronous pulley of the other side and tank body side connecting shaft to turn by synchronous belt Dynamic, connecting shaft rotation in tank body side then drives agitating paddle rotation in culture tank, and agitating paddle rotation is completed in culture tank The stirring of material.Motor side connecting shaft and tank body side connecting shaft pass through holding screw respectively and are fixedly connected with synchronous pulley.

Synchronous belt tension device is added in synchronous belt side, and synchronous belt tension device includes fixed block, fixed block and lower casing Plate is fixed, between fixed block and synchronous belt be equipped with tensioning block, tensioning block connection tensioning ball bearing inner race, tensioning ball bearing outer ring with Synchronous belt is close to；Spring is equipped between tensioning block and fixed block, spring middle position is equipped with spring shaft, spring shaft one end and tensioning Block is fixedly connected, and the spring shaft other end runs through fixed block, and is screwed with nut through spring shaft one end of fixed block.It is tensioned ball axis It honours synchronous belt to be tensioned, when synchronous belt movement, tensioning ball bearing outer ring is rotated with the movement of synchronous belt.With spring Compression, tensioning block, tensioning ball bearing, spring shaft can be moved forward and backward, and compressed specific location passes through solid on spring shaft The nut for determining block rear end is limited.Spring is initially in certain compressive state, under the action of the spring, tensioning block, tensioning Ball bearing and spring shaft are ejected by the elastic force of spring, and end nut after rotating clockwise on spring shaft, spring shaft moves backward, Tight block and spring axis connection, tensioning block and its bearing fixed move backward, and the distance between tensioning block and fixed block reduce, Spring between fixed block and tensioning block is compressed.Similarly, on torsional spring axis counterclockwise after end nut, tensioning block and solid Determine the increasing of the distance between block, amount of spring compression reduces.By the adjusting of nut, kept centainly between tensioning block and fixed block Distance, so that tensioning ball bearing has certain pressing force to synchronous, after running for a period of time, if synchronous belt has skidded Absent-mindedness, adjustable nut reduce the decrement of spring, spring elongation, and tensioning block and bearing stretch out under the left and right of spring force, Continue to keep the pressuring action to synchronous belt.

Preferably, the parallel cell culture tank includes tank body, and tank body top is equipped with cover, and tank body and cover pass through pressure Tight mode is fixed, and agitating paddle is equipped in tank body, and agitating paddle spindle top runs through cover；Feed supplement connector and air inlet are equipped with outside tank body Connector, feed supplement connector pass through cover by feed supplement branch pipe and are connected to tank interior, and inlet suction port is communicated in by external pipeline In cover or the intracorporal respective line of tank；Cover is equipped with sample tap, is placed with sampling plug engaged therewith in sample tap；Cover Equipped with PH electrode interface；O-ring seal is equipped between tank body and cover, tank end is inserted in O-ring seal, then by cover from Top of the tank indentation, under the action of O-ring seal, cover and tank body realize that ready-package is tightly connected, and the two disassembly is more square Just.

When parallel cell culture, tank body and cover are fixed by way of compressing O-ring seal, and agitating paddle spindle top connects Cans picking side connecting shaft.Feed supplement connector includes four tubular structures, is respectively worn by a feed supplement branch pipe at the top of four tubular structures It crosses cover and is connected to tank interior, four bottom part of tubular structure are correspondingly connected with other connectors, and four feed supplement branch pipes are respectively to tank body Interior supplement acid solution, lye, culture medium, defoaming agent.In parallel cell incubation, when needing to sample, plug will be sampled from sampling Mouth takes out, and completes again to put back to sampling plug after sampling and blocks sample tap, samples plug periphery and be equipped with circumferential seal circle, can By sample tap better seal.PH electrode interface surveys the pH value in culture solution in incubation for installing PH electrode Amount and control, require according to culture process, are passed through the amount of carbon dioxide in tank body by control to adjust the pH value of culture solution.Into Gas connector includes three tubular structures, and three tubular structures have been respectively communicated with surface layer air induction hose, tail gas condensing hose and bottom The other end of one end of air induction hose, surface layer air induction hose, tail gas condensing hose and bottom air induction hose is respectively communicated with set on tank Surface layer air inlet pipe in lid, the coiled coil hose in cover and the gas-distributing pipe for being connected to tank body bottom.Surface layer air induction hose The surface layer air inlet pipe being communicated in cover realizes the air inlet on surface layer；Tail gas condensing hose is communicated in the coiled coil in cover Hose according to the technological requirements, can be passed through air, oxygen or carbon dioxide in parallel cell incubation frequently in tank body, At this point, pressure can increase in tank body, in order to keep the stabilization of pressure inside the tank, exhaust pipe, i.e. tail gas condensing pipe are configured, meanwhile, it is It prevents from taking away moisture when exhaust, it is by cooling to cover, cover inside spin Coiled hose is cooling, so that hose Interior condensate moisture, comes back in tank body；Bottom air induction hose is connected to the gas-distributing pipe of tank body bottom, air, oxygen, titanium dioxide Carbon can be distributed to tank base by gas-distributing pipe, for microorganism or the culture medium of zooblast.

Tank interior bottom surface is equipped with locating shaft, and locating shaft is embedded in the positioning hole of agitating paddle main shaft bottom end, guarantees agitating paddle It runs more steady；And the main shaft lower part of agitating paddle is equipped with double-layer paddle, increases the uniformity of stirring.Tank body is in be arrived from above Under gradually smaller cone-shaped, and tank interior is circumferentially evenly equipped with baffle plate.

The medium position of bottom is equipped with annular groove in cover, is equipped with umbrella in umbrella-shaped structure around main shaft at the top of agitating paddle Shape ring, umbrella ring is in annular groove.Umbrella ring makes agitating paddle realize positioning, to guarantee to stir in annular groove The stable working state of paddle；And annular groove may further be used to realize the winding of coiled coil hose, guarantee coiled coil hose Position is more firm.

Preferably, the stirring motor is set in motor fixing seat, and motor fixing seat is fixedly connected with supporting frame, electricity Peltier cooler is equipped in machine fixing seat simultaneously, condensation fixed plate is equipped at the top of peltier cooler, condensation fixed plate is under The bottom face contact of lid plate bottom plate；The cover top surface of culture tank is close to setting with lower casing plate bottom surface simultaneously；Semiconductor cooling But device includes radiator fan, cold sink is equipped with above radiator fan, compressing between cold sink and condensation fixed plate has Cooling semiconductor, cooling semiconductor exterior circumferential is coated with heat insulating mattress, and lower casing plate is aluminum.

Aluminum products are more easier to absorb heat, radiate；Peltier cooler uses the anhydrous type of cooling, uses letter Single, easy to maintain, peltier cooler cools to lower casing plate, and temperature is lower and the lower casing plate that contacts with cover top surface Cover is cooling, and cover inside spin Coiled hose is cooled to cool down, and the moisture condensation in such hose flows back in tank body again Portion prevents the loss of moisture in tank in parallel cell incubation, and for small size parallel cell culture, this point is very It is important.

Tank body, cover, agitating paddle, feed supplement connector, inlet suction port and sampling plug are that PC material is made.Using PC material Material, is molded, and cost is lower, has disposable characteristic, eliminates before being not thorough and used due to sterilizing again The risk of pollution, and gas-distributing pipe etc. is that PC material is made.

Preferably, gas-liquid block is set above the supporting frame, is washed inside gas-liquid block equipped with lower feed supplement channel and row logical Gas-liquid block is run through in road, lower feed supplement channel, and row washes upper part of channel and is connected to by horizontal channel with lower feed supplement upper part of channel, and row washes channel Bottom end outlet is set to gas-liquid block bottom surface；Feed supplement connector is placed on gas-liquid block top, is equipped with upper feed supplement channel, upper feed supplement in feed supplement connector Channel upper end connects feed supplement branch pipe, and upper feed supplement channel lower end connects feed supplement tube head, and feed supplement tube head outer wall is equipped with long sealing ring, feed supplement Tube head carries long sealing ring and is inserted into lower feed supplement channel.

Feed supplement plug is added, feed supplement plug outer wall and feed supplement connector outer wall are equipped with semi-circular buckle, pass through when not in use Semi-circular buckle is sandwiched in inlet suction port periphery；Feed supplement plug bottom surface is equipped with block head, and block head periphery is enclosed with short sealing Circle, short sealing ring, which is not enough to arrange, washes channel enclosed, the closing of feed supplement plug upper surface；In incubation, it is pressed on gas-liquid block Feed supplement connector, feed supplement tube head carry long sealing ring and are inserted into lower feed supplement channel, since long sealing ring end is longer, row can be washed Channel enclosed, meanwhile, feed supplement pipe centriciput is hollow cylinder pore structure, required reagent in incubation, including acid, alkali, Culture solution, defoaming agent enter tank interior by lower feed supplement channel and upper feed supplement channel；After parallel cell culture, it will mend Material connector is removed from gas-liquid block, is stuck in inlet suction port periphery, later, the block head of feed supplement plug is respectively pressed on gas-liquid block Four lower feed supplement channels in, short sealing ring on block head seals lower feed supplement channel upper end, and feed supplement head fixing chuck plate will Feed supplement plug is fixed on gas-liquid block, at this point, gas-liquid block bottom pipe fitting is connected lower feed supplement channel enclosed by feed supplement plug Pipeline ejected wash water is switched to by solenoid valve by acid solution, lye, culture solution, then ejected wash water is along pipeline to pipe fitting, lower feed supplement Channel, horizontal channel are finally washed channel from row and are drained, and the cleaning process of pipeline is completed, can also be by gas-liquid block bottom after cleaning The pipeline that portion's pipe fitting is connected switches to high-temperature steam, and high-temperature steam equally along above-mentioned channel, carries out entire pipe-line system Sterilization treatment.It is fixed between gas-liquid block and feed supplement connector or by feed supplement head fixing chuck plate between gas-liquid block and feed supplement plug.

Preferably, the supporting frame below the tank body is equipped with stop sleeve, and stop sleeve includes outer sleeve and inner sleeve Cylinder, inner sleeve are set to inside outer sleeve, and cavity, cavity between outer sleeve and inner sleeve are equipped between inner sleeve and outer sleeve Partition is set at one, and partition two sides are respectively provided with a pagoda connector；Two pagoda connector one end are respectively communicated with the sky of partition two sides Chamber, the other end are separately connected corresponding pipeline, and outer sleeve is connected with the top of inner sleeve, and outer sleeve is fixedly connected with supporting frame. Outer sleeve is connected with inner sleeve by welding manner, and inner sleeve plays a supportive role to tank body, is formed between inner sleeve and outer sleeve Collet cavity, two pagodas connect first as import, another as outlet, can be passed through among collet cavity refrigerant or Heating agent, can the temperature to culture solution in incubation accurately controlled, may be implemented heat up and cool down double control, have The advantages that heating and cooling are rapid, and temperature uniformity is good.

Tank body lower part is placed in inner sleeve, and inner sleeve is cone-shaped cavity structure, outside inner sleeve cavity size and tank body Wall geomery matches, and inner sleeve upper surface is equipped with cone boss, and cone boss is stuck in the cone cell that tank body bottom surface is equipped with In hollow protrusion.Cone boss and the hollow protrusion of cone cell cooperate, and inner sleeve plays positioning action to tank body, guarantees tank body Positional accuracy, tank body position it is accurate, it is ensured that micro- lambda sensor patch and sensor at tank base arc groove Position is corresponding, accurate.

Bottom surface is equipped with arc groove outside tank body, and stop sleeve position corresponding with arc groove is equipped with micro- lambda sensor and connects Head, for fixing micro- lambda sensor probe.

Possessed beneficial effect is the utility model compared with prior art:

(1) include a number of parallel cell culture apparatus, big throughput cell culture can be completed；

(2) agitating paddle of each parallel cell culture apparatus is laterally driven by synchronous belt, the length dimension contracting of agitating shaft Small, whipping process is more stable, and package unit realizes that modularization, driving box body and motor and culture tank agitating paddle realize quickly peace Dress, the setting of synchronous belt tension device enable synchronous belt long-term stable operation；And peltier cooler is to being coiled in cover On coiled coil hose in steam condensed, prevent water loss in tank body；Using compression between cover and tank body Ready-package is connected and fixed, and cover components itself are less, after culture, culture solution topple over transfer it is more convenient；And tank Feed supplement connector and inlet suction port are connected in body and cover, feed operation is simpler efficiently；

(3) tank body, cover etc. are made of the molding of PC material molds, and cost is lower, have disposable characteristic, eliminate Since sterilizing is not thorough and using the preceding risk polluted again；

(4) gas-liquid block has binary channels, can either complete the delivery of therapeutic agents into tank body, and can be after culture to pipeline Carry out cleaning and sterilization treatment；And inner sleeve plays a supportive role to tank body, and collet cavity is formed between inner sleeve and outer sleeve, Two pagodas connect first as import, another, can be to being passed through refrigerant or heating agent among collet cavity as outlet, can be with The temperature of culture solution in incubation is accurately controlled, the double control that heats up and cool down may be implemented, have heating and cooling fast The advantages that speed, temperature uniformity is good

(5) liquid relief sampler replaces manual operation, makes entirely to cultivate region and is among clean environment, may be implemented pair The automatic liquid relief sampling of reagent and auxiliary material fill operation in culture tank body, and have high-precision and good repeatability, certainly Without manual operation in dynamic pipetting processes, the working environment of more safety and hommization has been built.

Detailed description of the invention

The automatic training station structure schematic diagram (main view one) of Fig. 1, parallel cell；

The automatic training station structure schematic diagram (top view) of Fig. 2, parallel cell；

The automatic training station structure schematic diagram (main view two) of Fig. 3, parallel cell；

The automatic training station structure schematic diagram (left view) of Fig. 4, parallel cell；

Fig. 5, A partial enlarged view (supporting vertical plate main view)；

Fig. 6, supporting vertical plate rearview；

Fig. 7, parallel cell culture apparatus structural schematic diagram (perspective view)；

Fig. 8, parallel cell culture apparatus structural schematic diagram (top view)；

Fig. 9, B-B cross-sectional view；

Figure 10, parallel cell culture tank structural schematic diagram (main view)；

Figure 11, parallel cell culture tank structural schematic diagram (top view)；

Figure 12, C-C cross-sectional view；

Structural schematic diagram in Figure 13, lower casing plate；

Figure 14, pagoda joint position structure schematic diagram；

Partial enlarged view at Figure 15, driving box body；

Figure 16, gas-liquid block and feed supplement plug fit structure schematic diagram；

Figure 17, pipette tips, which are moved back, unloads component structure diagram；

Figure 18, sleeve covering plate structure schematic diagram.

In figure: 1, working station frame；2, vessel rest area；3, culture solution holding vessel rest area；4, parallel cell culture Device；5, liquid relief sampler；6,24 orifice plate plastic lid mounting table；7, pipette tips store area；8, pipette tips, which are moved back, unloads component；9, centrifuge tube Area；10,24 orifice plate area；11, supporting vertical plate；12, X-axis linear guide；13, X-axis motor driver；14, transverse slat is supported；15, Y-axis Motor driver；16, Y-axis support frame；17, gripper lead screw stepper motor；18, sliding rail one；19, the silk of gripper lead screw stepper motor Bar；20, electrical clip grasps fixed board；21, electronic gripper；22, liquid-transfering gun；23, pipette tips sleeve briquetting；24, pipette tips sleeve；25, spiral shell Line casing；26, pipette tips sleeve fixed plate；27, the screw rod of pipette tips lead screw stepper motor；28, sliding rail two；29, pipette tips lead screw stepping Motor；30, sucker lead screw stepper motor；31, the screw rod of sucker lead screw stepper motor；32, sliding rail three；33, sucker fixed plate； 34, sucker founds side plate；35, sucker vacuum box；36, vacuum chuck；37, motor overturning plate；38, rotating stepper motor；39, spiral Hose；40, muffler；41, minipump；42, supporting frame；43, inlet suction port；44, tank body；45, motor fixing seat； 46, box body is driven；47, feed supplement plug；48, feed supplement connector；49, feed supplement head fixing chuck plate；50, gas-liquid block；51, PH electrode connects Mouthful；52, plug is sampled；53, upper box cover plate；54, synchronous pulley；55, bearing one；56, motor side connecting shaft；57, bearing two； 58, lower casing plate；59, cold sink；60, radiator fan；61, stirring motor；62, outer sleeve；63, inner sleeve；64, baffling Plate；65, the hollow protrusion of cone cell；66, cone boss；67, micro- lambda sensor connector；68, gas-distributing pipe；69, partition；70, pagoda connects Head；71, row washes channel；72, lower feed supplement channel；73, horizontal channel；74, upper feed supplement channel；75, agitating paddle；76, feed supplement branch pipe； 77, umbrella ring；78, annular groove；79, cover；80, heat insulating mattress；81, tank body side connecting shaft；82, cooling semiconductor；83, it condenses Fixed plate；84, long sealing ring；85, surface layer air induction hose；86, tail gas condensing hose；87, bottom air induction hose；88, O-shaped sealing Circle；89, locating shaft；90, fixed block；91, spring；92, spring shaft；93, tensioning block；94, synchronous belt；95, it is tensioned ball bearing； 96, nut；97, semi-circular buckles；98, sleeve cover board；99, withdrawal sleeve cylinder；100, circular hole；101, square hole；102, block head.

Specific embodiment

The utility model is described further with reference to the accompanying drawing:

Such as Fig. 1-Figure 18, the automatic training station of parallel cell described in the utility model, including working station frame, work The lower plate upper surface for making station body frame is set there are three region, respectively parallel cell cultivation region, vessel rest area 2 and culture solution Holding vessel rest area 3, working station frame upper end are equipped with the liquid relief sampler 5 with these three region cooperatings；It is parallel thin A number of parallel cell culture apparatus 4 is equipped in born of the same parents cultivation region.

In the present embodiment:

Liquid relief sampler 5 includes X-axis moving assembly and Y-axis moving assembly, Y-axis moving assembly and X-axis moving assembly Sliding block is fixedly connected, and the sliding block of Y-axis moving assembly is fixedly connected with support transverse slat 14, and support 14 bottom surface of transverse slat is fixedly connected with support Vertical plate 11,11 surface of supporting vertical plate are provided with pipette tips moving assembly, gripper moving assembly and sucker moving assembly.X-axis moves group Part includes horizontally disposed X-axis motor driver 13 and X-axis linear guide 12, and Y-axis moving assembly includes 16 He of Y-axis support frame Y-axis motor driver 15, the sliding block of X-axis motor driver 13 and the sliding block of X-axis linear guide 12 connect Y-axis support frame simultaneously 16, the vertical X axis linear guide 12 in the horizontal direction of Y-axis support frame 16 is equipped with Y-axis motor driver 15 below Y-axis support frame 16, The sliding block of Y-axis motor driver 15 is fixedly connected with support transverse slat 14.X-axis motor driver 13 acts, and drives 16 edge of Y-axis support frame X-axis linear guide 12 is moved to designated position；Y-axis motor driver 15 acts, and support transverse slat 14 is driven to be moved to designated position.

Gripper moving assembly includes gripper lead screw stepper motor 17 and sliding rail 1, the screw rod 19 of gripper lead screw stepper motor It is arranged in parallel with sliding rail 1, the sliding block one in the nut and sliding rail 1 of gripper lead screw stepper motor 17 connects electrical clip simultaneously Fixed board 20 is grasped, electrical clip grasps 20 bottom end of fixed board and is provided with electronic gripper 21；Gripper lead screw stepper motor 17 rotates forward, nut band Dynamic electrical clip grasps fixed board 20 and moves down along sliding rail 1, and electrical clip grasps fixed board 20 and electronic gripper 21 is driven to be moved downward to Required crawl position, then component needed for electronic 21 motion capture of gripper, completes the reversion of gripper lead screw stepper motor 17 after work, Electronic gripper 21 resets；

Pipette tips moving assembly includes pipette tips lead screw stepper motor 29 and sliding rail 2 28, the screw rod 27 of pipette tips lead screw stepper motor It is arranged in parallel with sliding rail 2 28, the sliding block two in the nut and sliding rail 2 28 of pipette tips lead screw stepper motor 29 connects pipette tips set simultaneously Cylinder fixed plate 26, pipette tips sleeve 24 are located in 26 bottom end of pipette tips sleeve fixed plate, pipette tips set by the compression of pipette tips sleeve briquetting 23 24 tops of cylinder are equipped with threaded sleeve 25, and threaded sleeve 25 connects 5ml syringe pump, 5ml syringe pump by syringe pump step motor control, Syringe pump stepper motor is controlled by control system, and pipette tips lead screw stepper motor 29 rotates forward, and nut drives pipette tips sleeve fixed plate 26 It is moved down along sliding rail 2 28, pipette tips sleeve fixed plate 26 drives pipette tips sleeve 24 to be moved to vessel rest area 2, pipette tips sleeve 24 Liquid-transfering gun 22 is obtained out of vessel rest area 2, then X-axis moving assembly and the movement of Y-axis moving assembly drive liquid-transfering gun 22 mobile To sample position, sampling work is completed；

Sucker moving assembly includes sucker lead screw stepper motor 30 and sliding rail 3 32, the screw rod 31 of sucker lead screw stepper motor It is arranged in parallel with sliding rail 3 32, the sliding block San Tong method connecting sucker in the nut and sliding rail 3 32 of sucker lead screw stepper motor 30 is solid Fixed board 33,33 side of sucker fixed plate are equipped with sucker and found side plate 34, and sucker founds 34 bottom end connecting sucker vacuum box 35 of side plate, sucker 35 bottom surface of vacuum box connects vacuum chuck 36, and 35 upper surface connector of sucker vacuum box is connected to minipump by corrugated hose 39 41 vacuum port, the exhaust outlet joint noise reduction device 40 of minipump 41；Sucker lead screw stepper motor 30 rotates forward, and nut, which drives, to be inhaled Disk fixed plate 33 moves down simultaneously along sliding rail 3 32, and sucker fixed plate 33 drives sucker to found side plate 34,35 and of sucker vacuum box Vacuum chuck 36 moves down, and corrugated hose 39 is stretched during sucker vacuum box 35 moves down, when vacuum chuck 36 connects When contacting the corresponding component in vessel rest area 2, minipump 41 is acted, and exhaust outlet is vented outwards by muffler 40, Its vacuum port air-breathing generates vacuum, and the corrugated hose 39 and sucker vacuum box 35 and vacuum chuck 36 for connecting vacuum port come true Sky, so that corresponding component is sucked vacuum chuck 36, X-axis moving assembly and the movement of Y-axis moving assembly cooperate vacuum chuck 36 Corresponding component is sucked to remove.

Pipette tips lead screw stepper motor 29, gripper lead screw stepper motor 17, electronic gripper 21, sucker lead screw stepper motor 30, Minipump 41 is all connected with control system.11 bottom of supporting vertical plate is equipped with rotating stepper motor 38, and rotating stepper motor 38 is defeated Shaft bottom end is connected and fixed motor overturning plate 37, and 37 upper surface of motor overturning plate is equipped with fixed sponge, and motor overturning plate 37 is located at It is not contacted below supporting vertical plate 11 and directly with supporting vertical plate 11, when rotating stepper motor 38 rotates forward, motor overturning plate 37 22 lower section of liquid-transfering gun carried to pipette tips sleeve 24 is overturn, when X-axis motor driver 13, Y-axis motor driver 15 are mobile, such as There is 22 dropping liquid of liquid-transfering gun in fruit, and the drop of drippage can fall in the sponge of 37 upper surface of motor overturning plate immediately below liquid-transfering gun 22 On, prevent drips in other regions, when rotating stepper motor 38 inverts, motor overturning plate 37 overturns vertical to supporting again 11 lower section of plate, completely offsets from immediately below liquid-transfering gun 22, interference effect will not be generated to the decline of liquid-transfering gun 22, not to other devices Movement impact.

Vessel platform is equipped in vessel rest area 2, vessel platform top is equipped with 24 orifice plate areas 10, pipette tips storage area 7 and centrifuge tube 9,24 orifice plate area, area, 10 side is equipped with 24 orifice plate plastic lid mounting tables 6, and pipette tips storage 7 side of area is moved back equipped with pipette tips unloads component 8.24 It is equipped with 24 hole grillage everywhere in orifice plate area 10,24 orifice plates are equipped in 24 hole grillages, is stamped 24 orifice plate plastic lids above 24 orifice plates, 24 10 side of orifice plate area is equipped with 24 orifice plate plastic lid mounting tables 6；Pipette tips, which are stored, is equipped with pipette tips support frame in area 7, in pipette tips support frame Several liquid-transfering guns 22 are placed with, pipette tips storage 7 side of area is moved back equipped with pipette tips unloads component 8；It is centrifuged in area under control 9 and is equipped with centrifuge tube shelf, Several centrifuge tubes are placed in centrifuge tube shelf.

Parallel cell culture apparatus 4 includes supporting frame 42, and culture tank stirring driving dress is provided with above supporting frame 42 It sets and includes driving box body 46 with parallel bioreactor, culture tank mixer drive, both ends in box body 46 is driven to be respectively equipped with Motor side connecting shaft 56 and tank body side connecting shaft 81, motor side connecting shaft 56 and tank body side connecting shaft 81 are separately connected stirring motor 61 output shafts and the agitating paddle 75 in parallel cell culture tank.Supporting frame 42 includes Left-side support plate and Right side support Plate, Left-side support plate and Right side support plate are fixedly mounted by positioning pin and main equipment, to culture tank mixer drive and Parallel cell culture tank plays a supportive role.

Driving box body 46 includes upper box cover plate 53 and lower casing plate 58, and upper box cover plate 53 and the composition of lower casing plate 58 drive box 46 shell of body drives the both ends inside box body 46 to be respectively provided with a synchronous pulley 54, passes through synchronization between two synchronous pulleys 54 Band 94 connects, and two 54 tops of synchronous pulley be embedded ins 53 top surface of upper box cover plate, and synchronous pulley 54 and upper box cover plate 53 it Between be equipped with bearing 1；Motor side connecting shaft 56,56 bottom of motor side connecting shaft are fixed in the middle part of one of synchronous pulley 54 Portion passes through 58 bottom plate of lower casing plate, passes through the output axis connection of shaft coupling and stirring motor 61, another 54 middle part of synchronous pulley It is fixed with tank body side connecting shaft 81, connecting shaft 81 bottom in tank body side passes through 58 bottom plate of lower casing plate, in culture tank 44 Agitating paddle 75 connects；Motor side connecting shaft 56 and tank body side connecting shaft 81 are equipped with bearing 2 57 between lower casing plate 58 respectively.

When installation, first stirring motor 61 is fixed on supporting frame 42, parallel cell culture tank is placed on supporting frame On 42, then install again driving box body 46, make drive box body 46 motor side connecting shaft 56 and tank body side connecting shaft 81 respectively with Electric machine main shaft and culture tank agitating paddle 75 are aligned simultaneously, Fast Installation, complete connection.During driving, electric machine main shaft rotates band Dynamic motor side connecting shaft 56 and the synchronous pulley 54 being secured to rotate, and the synchronous pulley 54 of this side is driven separately by synchronous belt 94 The synchronous pulley 54 and tank body side connecting shaft 81 of side rotate, and the rotation of tank body side connecting shaft 81 then drives in culture tank 44 and stirs The rotation of paddle 75 is mixed, the stirring to material in culture tank 44 is completed in the rotation of agitating paddle 75.Motor side connecting shaft 56 and tank body side Connecting shaft 81 is fixedly connected by holding screw with synchronous pulley 54 respectively.

94 tensioning apparatus of synchronous belt is added in 94 side of synchronous belt, and 94 tensioning apparatus of synchronous belt includes fixed block 90, fixed block 90 is fixed with lower casing plate 58, and tensioning block 93, the connection tensioning ball bearing of tensioning block 93 are equipped between fixed block 90 and synchronous belt 94 95 inner rings, tensioning 95 outer ring of ball bearing are close to synchronous belt 94；Spring 91, spring 91 are equipped between tensioning block 93 and fixed block 90 Middle position is equipped with spring shaft 92, and 92 one end of spring shaft is fixedly connected with tensioning block 93, and 92 other end of spring shaft runs through fixed block 90, and nut 96 is screwed with through 92 one end of spring shaft of fixed block 90.Tensioning ball bearing 95 is tensioned synchronous belt 94, when When synchronous belt 94 moves, tensioning 95 outer ring of ball bearing is rotated with the movement of synchronous belt 94.With the compression of spring 91, tensioning block 93, tensioning ball bearing 95, spring shaft 92 can be moved forward and backward, and compressed specific location passes through fixed block 90 on spring shaft 92 The nut 96 of rear end is limited.Spring 91 is initially in certain compressive state, under the action of spring 91, tensioning block 93, Tensioning ball bearing 95 and spring shaft 92 are ejected by the elastic force of spring 91, end nut 96 after rotating clockwise on spring shaft 92, bullet Spring axis 92 moves backward, and tensioning block 93 is connect with spring shaft 92, and tensioning block 93 and its bearing fixed move backward, tensioning block The distance between 93 and fixed block 90 reduce, and the spring 91 between fixed block 90 and tensioning block 93 is compressed.Similarly, the inverse time End nut 96 after on needle torsional spring axis 92, the distance between tensioning block 93 and fixed block 90 increase, and 91 decrement of spring reduces. It by the adjusting of nut 96, is maintained a certain distance between tensioning block 93 and fixed block 90, so that tensioning 95 pairs of synchronizations of ball bearing There is certain pressing force with 94, after running for a period of time, if synchronous belt 94 has skidded absent-mindedness, adjustable nut 96, The decrement of spring 91 is reduced, spring 91 extends, and tensioning block 93 and bearing stretch out under the left and right of 91 power of spring, continues holding pair The pressuring action of synchronous belt 94.

Parallel cell culture tank includes tank body 44, and 44 top of tank body is equipped with cover 79, and tank body 44 and cover 79 pass through compression Mode is fixed, and agitating paddle 75 is equipped in tank body 44, and 75 spindle top of agitating paddle runs through cover 79；44 outside of tank body connects equipped with feed supplement First 48 and inlet suction port 43, feed supplement connector 48 passes through cover 79 by feed supplement branch pipe 76 and is connected to inside tank body 44, inlet suction port 43 are communicated in the respective line in cover 79 or in tank body 44 by external pipeline；Cover 79 is equipped with sample tap, in sample tap It is placed with sampling plug 52 engaged therewith；Cover 79 is equipped with PH electrode interface 51；It is equipped between tank body 44 and cover 79 O-shaped close O-ring seal 88 is inserted in seal 88,44 end of tank body, is then pressed into cover 79 at the top of tank body 44, in O-ring seal 88 Under effect, cover 79 and tank body 44 realize that ready-package is tightly connected, and the two disassembly is more convenient.

When parallel cell culture, tank body 44 is fixed by way of compressing O-ring seal 88 with cover 79, and agitating paddle 75 is main Tank body side connecting shaft 81 is connected at the top of axis.Feed supplement connector 48 includes four tubular structures, respectively passes through one at the top of four tubular structures Root feed supplement branch pipe 76 passes through cover 79 and is connected to inside tank body 44, and four bottom part of tubular structure are correspondingly connected with other connectors, and four Feed supplement branch pipe 76 supplements acid solution, lye, culture medium, defoaming agent into tank body 44 respectively.In parallel cell incubation, need to take When sample, sampling plug 52 is taken out from sample tap, completes again to put back to sampling plug 52 after sampling and block sample tap, sampling is stifled First 52 periphery is equipped with circumferential seal circle, can be by sample tap better seal.PH electrode interface 51 is for installing PH electrode, to training PH value during supporting in culture solution is measured and is controlled, and is required according to culture process, is passed through two in tank body 44 by control The amount of carbonoxide adjusts the pH value of culture solution.Inlet suction port 43 includes three tubular structures, and three tubular structures are respectively communicated with There are one end of surface layer air induction hose 85, tail gas condensing hose 86 and bottom air induction hose 87, surface layer air induction hose 85, tail gas condensing The other end of hose 86 and bottom air induction hose 87 is respectively communicated with surface layer air inlet pipe in cover 79, in cover 79 Coiled coil hose and the gas-distributing pipe 68 for being connected to 44 bottom of tank body.Surface layer air induction hose 85 be communicated in the surface layer in cover 79 into The air inlet on tracheae realization surface layer；Tail gas condensing hose 86 is communicated in the coiled coil hose in cover 79, trains in parallel cell During supporting, according to the technological requirements, air, oxygen or carbon dioxide can be passed through in tank body 44 frequently, at this point, 44 internal pressure of tank body Power can increase, and in order to keep the stabilization of pressure inside the tank, configure exhaust pipe, i.e. tail gas condensing pipe 86, meanwhile, when being vented in order to prevent Moisture is taken away, it is by cooling to cover 79,79 inside spin Coiled hose of cover is cooling, so that the moisture in hose Condensation, comes back in tank body 44；Bottom air induction hose 87 is connected to the gas-distributing pipe 68 of 44 bottom of tank body, air, oxygen, titanium dioxide Carbon can be distributed to 44 bottom of tank body by gas-distributing pipe 68, for microorganism or the culture medium of zooblast.

44 inner bottom surface of tank body is equipped with locating shaft 89, and locating shaft 89 is embedded in the positioning hole of 75 main shaft bottom end of agitating paddle, protects It is more steady to demonstrate,prove the operation of agitating paddle 75；And the main shaft lower part of agitating paddle 75 is equipped with double-layer paddle, increases the uniformity of stirring. Tank body 44 is in gradually smaller cone-shaped from top to bottom, and 44 internal circumferential of tank body is evenly equipped with baffle plate 64.

The medium position of bottom is equipped with annular groove in cover 79, sets around 75 top main shaft of agitating paddle in umbrella-shaped structure There is umbrella ring 77, umbrella ring 77 is in annular groove 78.Umbrella ring 77 realizes agitating paddle 75 fixed in annular groove 78 Position, to guarantee the stable working state of agitating paddle 75；And annular groove 78 may further be used to realize twining for coiled coil hose Around guarantee coiled coil hose position is more firm.

Stirring motor 61 is set in motor fixing seat 45, and motor fixing seat 45 is fixedly connected with supporting frame 42, and motor is solid Peltier cooler is equipped in reservation 45 simultaneously, is equipped with condensation fixed plate 83 at the top of peltier cooler, condensation fixed plate 83 with The bottom face contact of 58 bottom plate of lower casing plate；79 top surface of cover of culture tank is close to setting with 58 bottom surface of lower casing plate simultaneously； Peltier cooler includes radiator fan 60, and cold sink 59, cold sink 59 and condensation are equipped with above radiator fan 60 Compressing between fixed plate 83 has cooling semiconductor 82, and cooling 82 exterior circumferential of semiconductor is coated with heat insulating mattress 80, and lower casing Plate 58 is aluminum.

Aluminum products are more easier to absorb heat, radiate；Peltier cooler uses the anhydrous type of cooling, uses letter Single, easy to maintain, peltier cooler cools to lower casing plate 58, and temperature is lower and the lower box that contacts with 79 top surface of cover Cover board 58 is cooling by cover 79, the cooled cooling of 79 inside spin Coiled hose of cover, and the moisture condensation in such hose flows again It returns inside tank body 44, prevents the loss of moisture in tank in parallel cell incubation, for small size parallel cell culture, This point is particularly significant.

Tank body 44, cover 79, agitating paddle 75, feed supplement connector 48, inlet suction port 43 and sampling plug 52 are PC material system At.Using PC material, be molded, cost is lower, have disposable characteristic, eliminate due to sterilizing be not thorough and Using the preceding risk polluted again, and gas-distributing pipe 68 etc. is that PC material is made.

Gas-liquid block 50 is set above supporting frame 42, is equipped with lower feed supplement channel 72 inside gas-liquid block 50 and row washes channel 71, Gas-liquid block 50 is run through in lower feed supplement channel 72, and row washes 71 top of channel and is connected to by horizontal channel 73 with 72 top of lower feed supplement channel, Row washes 71 bottom end outlet of channel set on 50 bottom surface of gas-liquid block；Feed supplement connector 48 is placed on 50 top of gas-liquid block, is set in feed supplement connector 48 There is upper feed supplement channel 74, upper 74 upper end of feed supplement channel connects feed supplement branch pipe 76, and upper 74 lower end of feed supplement channel connects feed supplement tube head, mends Expects pipe head outer wall is equipped with long sealing ring 84, and feed supplement tube head carries long sealing ring 84 and is inserted into lower feed supplement channel 72.

Feed supplement plug 47 is added, 47 outer wall of feed supplement plug and 48 outer wall of feed supplement connector are equipped with semi-circular buckle, do not use When by semi-circular buckle be sandwiched in inlet suction port periphery；47 bottom surface of feed supplement plug is equipped with block head 102,102 periphery of block head It is enclosed with short sealing ring, short sealing ring, which is not enough to arrange, to be washed channel 71 and close, the closing of 47 upper surface of feed supplement plug；It cultivated Cheng Zhong is pressed into feed supplement connector 48 on gas-liquid block 50, and feed supplement tube head carries long sealing ring 84 and is inserted into lower feed supplement channel 72, due to length 84 end of sealing ring is longer, and row can be washed to channel 71 and closed, meanwhile, feed supplement pipe centriciput is hollow cylinder pore structure, culture Required reagent in the process, including acid, alkali, culture solution, defoaming agent are entered by lower feed supplement channel 72 and upper feed supplement channel 74 Tank interior；After parallel cell culture, feed supplement connector 48 is removed from gas-liquid block 50, is stuck in inlet suction port periphery, Later, in four lower feed supplement channels 72 block head 102 of feed supplement plug 47 being respectively pressed on gas-liquid block 50, block head 102 On short sealing ring lower 72 upper end of feed supplement channel is sealed, feed supplement plug 47 is fixed on gas-liquid block by feed supplement head fixing chuck plate 49 On 50, at this point, feed supplement plug 47 closes lower feed supplement channel 72, the pipeline that 50 bottom pipe fitting of gas-liquid block is connected is by acid Liquid, lye, culture solution switch to ejected wash water by solenoid valve, then ejected wash water is along pipeline to pipe fitting, lower feed supplement channel 72, level Channel 73 is finally washed channel 71 from row and is drained, completes the cleaning process of pipeline, can also be by 50 bottom of gas-liquid block after cleaning The pipeline that pipe fitting is connected switches to high-temperature steam, and high-temperature steam equally along above-mentioned channel, goes out to entire pipe-line system Bacterium processing.Pass through feed supplement head fixing chuck plate 49 between gas-liquid block 50 and feed supplement connector 48 or between gas-liquid block 50 and feed supplement plug 47 It is fixed.

The supporting frame 42 of the lower section of tank body 44 is equipped with stop sleeve, and stop sleeve includes outer sleeve 62 and inner sleeve 63, interior Sleeve 63 is set to inside outer sleeve 62, and cavity, outer sleeve 62 and inner sleeve 63 are equipped between inner sleeve 63 and outer sleeve 62 Between partition 69 is set at cavity one, 69 two sides of partition are respectively provided with a pagoda connector 70；Two 70 one end of pagoda connector difference It is connected to the cavity of 69 two sides of partition, the other end is separately connected corresponding pipeline, and outer sleeve 62 is connected with the top of inner sleeve 63, housing Cylinder 62 is fixedly connected with supporting frame 42.Outer sleeve 62 is connected with inner sleeve 63 by welding manner, and inner sleeve 63 is to tank body 44 It plays a supportive role, forms collet cavity between inner sleeve 63 and outer sleeve 62, two pagoda connector 70 1 are used as import, another It is a as outlet, can to being passed through refrigerant or heating agent among collet cavity, can the temperature to culture solution in incubation carry out Accurate control may be implemented the double control that heats up and cool down, have many advantages, such as that heating and cooling are rapid, temperature uniformity is good.

44 lower part of tank body is placed in inner sleeve 63, inner sleeve 63 be cone-shaped cavity structure, inner sleeve cavity size with 44 outer wall geomery of tank body matches, and 63 upper surface of inner sleeve is equipped with cone boss 66, and cone boss 66 is stuck in tank body In the cone cell that 44 bottom surfaces are equipped with hollow raised 65.Cone boss 66 and cone cell hollow raised 65 cooperate, and inner sleeve 63 is to tank Body 44 plays positioning action, guarantees the positional accuracy of tank body 44,44 position of tank body it is accurate, it is ensured that 44 bottom arc of tank body Micro- lambda sensor patch is corresponding with the position of sensor, accurate at connected in star.

44 outside bottom surface of tank body is equipped with arc groove, and stop sleeve position corresponding with arc groove is equipped with micro- lambda sensor Connector 67, for fixing micro- lambda sensor probe.

Before parallel cell culture, feed supplement plug 47 is pressed into gas-liquid block 50, feed supplement plug 47 has short sealing ring, short Sealing ring length is shorter, washes channel 71 to row and washes the culture of 71 pairs of channel by row without closing, ejected wash water or high-temperature steam Pipeline in the process carries out cleaning and sterilization treatment, will be by the parallel cell of irradiation sterilization after the completion of cleaning and sterilization treatment Culture tank is taken out out of sealed plastic bag, then places tank body 44 in inner sleeve 63, by feed supplement plug 47 from gas-liquid block 50 It removes, the feed supplement tube head for being equipped with long sealing ring 84 is pressed into gas-liquid block 50, and the two is solid by feed supplement head fixing chuck plate 49 Level pressure is tight, and inlet suction port 43 is nested with the intake interface at 50 top of gas-liquid block and is docked, and parallel cell culture tank is installed, Then stirring motor 61 is docked by driving box body 46 with agitating paddle 75；Each difference fills the culture solution storage of different reagents Tank is correspondingly connected with feed supplement connector 48 and inlet suction port 43 by different pipelines respectively, and solenoid valve is equipped at tube outlet, electricity Magnet valve is electrically connected connected control system, and the culture medium in culture solution holding vessel is in tank under the action of aseptic compressed air, by electromagnetism Valve enters in tank, and stirring motor 61 works, and 75 work of agitating paddle in parallel cell culture tank is then driven by driving box body 46 To make, agitating paddle 75 stirs the culture medium in tank body 44, and parallel cell culture starts, in parallel cell incubation, acid solution, alkali Liquid and defoaming agent are under the control of existing program, in quantitative filling to tank body 44, three kinds of air, oxygen, carbon dioxide gaseous states Medium is passed into tank body 44 by inlet suction port 43, gas-distributing pipe 68.Training in PH electrode and micro- lambda sensor real time monitoring tank body 44 Relevant parameter in nutrient solution, and PLC is fed back to, the addition time and dosage of the above liquid of process control and gaseous medium；It is training Support during, when such as needing to improve culture-liquid temp, then by a pagoda connector 70 be passed through into collet cavity high-temperature water into Row heating, by heat transfer, the tank body 44 and culture solution being placed in stop sleeve are heated up, equally, when needs are to training When nutrient solution is cooled down, then water at low temperature is passed through into collet cavity by a pagoda connector 70 and realizes cooling.

Reagent needed for culture solution holding vessel is provided by the pump housing, pipeline feed supplement connector and inlet suction port into culture tank.Just When beginning state, the sliding block of X-axis motor driver 13 and the sliding block of Y-axis motor driver 15 are respectively positioned on the zero point position of program setting It sets, in addition, each displacement point of complete equipment is labelled with absolute coordinate, i.e., each displacement point is carried out by abscissa and ordinate Location position；Displacement point includes each liquid storage hole in 24 orifice plates on vessel platform, every centrifuge tube, each liquid-transfering gun 22,24 Orifice plate plastic lid mounting table 6, pipette tips move back the position for unloading 4 inner tank body 44 of component 8 and each parallel cell culture apparatus；In addition, Relative position between pipette tips sleeve 24, electronic gripper 21 and vacuum chuck 36 is also labeled.

When control system, which issues signal, to be sampled operation in the tank body 44 to some parallel cell culture apparatus 4, Under acting on while X-axis motor driver 13 and Y-axis motor driver 15, pipette tips sleeve 24 is moved to some liquid relief of setting Right above rifle 22, pipette tips lead screw stepper motor 29 is rotated forward, and nut drives pipette tips sleeve fixed plate 26 to move down along sliding rail 2 28, Liquid-transfering gun 22 is inserted in 24 bottom end of pipette tips sleeve, and then pipette tips lead screw stepper motor 29 inverts, and pipette tips sleeve 24 carries liquid-transfering gun 22 It moves up, subsequent X-axis motor driver 13 and the cooperation movement of Y-axis motor driver 15, so that electronic gripper 21 is moved to and takes Right above sample plug 52, the grappling fixture that control system controls electronic gripper 21 is opened, and then gripper lead screw stepper motor 17 rotates forward, spiral shell Master tape moves electrical clip and grasps fixed board 20 and move down along sliding rail 1, and electrical clip grasps fixed board 20 and drives electronic gripper 21 to moving down It moves to sampling plug 52, the closure that control system controls the grappling fixture of electronic gripper 21 just clamps sampling plug 52, then Gripper lead screw stepper motor 17 inverts, and electronic gripper 21 moves up, and is detached from so that sampling plug 52 with cover 79, then X-axis Motor driver 13 and Y-axis motor driver 15 cooperate movement again, so that liquid-transfering gun 22 is moved to right above sample tap, then Pipette tips lead screw stepper motor 29 rotates forward again, and pipette tips sleeve 24 drives liquid-transfering gun 22 to move down, when the sampled mouth of liquid-transfering gun 22 When being deep into tank body 44,24 top of pipette tips sleeve is mobile by the piston rod for the 5ml syringe pump that threaded sleeve 25 connects, and carries out Liquid extracting, culture solution enter in liquid-transfering gun 22, and extraction amount minimum can achieve 0.3ml, and (culture solution of extraction will not enter 5ml syringe pump, therefore 5ml syringe pump does not need to change) then 29 reversion of pipette tips lead screw stepper motor, drive liquid-transfering gun 22 to move up, It is removed out of tank body 44；Then rotating stepper motor 38 acts, make motor overturning plate 37 turn to immediately below liquid-transfering gun 22 (if There is 22 dropping liquid of liquid-transfering gun, the drop of drippage can be fallen on the sponge of 37 upper surface of motor overturning plate immediately below liquid-transfering gun 22, Prevent drips in other regions), then X-axis motor driver 13 and Y-axis motor driver 15 act, and move liquid-transfering gun 22 It moves to certain centrifuge tube, then 38 counteragent of rotating stepper motor, motor overturning plate 37 deviates liquid-transfering gun 22, subsequent rifle Captacula thick stick stepper motor 29 rotates forward, and liquid-transfering gun 22 is moved in centrifuge tube, and the piston rod reverse movement of control 5ml syringe pump is moved Culture solution in liquid rifle 22 is injected into centrifuge tube, and then pipette tips lead screw stepper motor 29 inverts, and liquid-transfering gun 22 is from centrifuge tube Middle removal；X-axis motor driver 13 and Y-axis motor driver 15 act, and so that liquid-transfering gun 22 is moved to pipette tips and is moved back and unload on component 8 Side completes the removal of liquid-transfering gun 22.It includes withdrawal sleeve cylinder 99 that pipette tips, which move back and unload component 8, is equipped with sleeve cover board above withdrawal sleeve cylinder 99 98, sleeve cover board 98, which is equipped with, is connected to the slotted hole formed with square hole 101 by circular hole 100, and the width of square hole 101 is less than circular hole 100 diameter, while the width of square hole 101 is less than the outer diameter of liquid-transfering gun 22, the width of square hole 101 is greater than liquid-transfering gun 22 and pipette tips The link position of sleeve 24.When 22 removal of liquid-transfering gun, the region between pipette tips sleeve 24 and liquid-transfering gun 22, which is caught in pipette tips, moves back and unloads group In 101 slot of square hole in part 8, pipette tips lead screw stepper motor 29 is inverted, and pipette tips sleeve 24 moves up, and the top of liquid-transfering gun 22 exists Below 101 slot of square hole, it can not be removed out of slot, liquid-transfering gun 22 is unloaded from pipette tips sleeve 24.

When control system, which is issued, carries out pipetting to some tank body 44, X-axis motor driver 13 and Y-axis electric drive Device 15 acts, so that vacuum chuck 36 is moved to right above 24 orifice plates, then sucker lead screw stepper motor 30 is rotated forward, and nut drives Sucker fixed plate 33 moves down simultaneously along sliding rail 3 32, when vacuum chuck 36 touches 24 orifice plate plastic lid, micro vacuum 41 movement of pump, so that 24 orifice plate plastic lids are sucked vacuum chuck 36, sucker lead screw stepper motor 30 is inverted, and vacuum chuck 36 is taken 24 orifice plate plastic lid of band moves up, and subsequent X-axis motor driver 13 and Y-axis motor driver 15 act, and makes 24 orifice plate plastics Lid is moved to 24 orifice plate plastic lid mounting table, 6 top, and then sucker lead screw stepper motor 30 rotates forward, and vacuum chuck 36 carries 24 holes Plate plastic lid moves down, and subsequent 41 stopping of minipump movement, vacuum is eliminated, and 24 orifice plate plastic lids are placed in the modeling of 24 orifice plates Expect on lid mounting table 6；Then under acting on while X-axis motor driver 13 and Y-axis motor driver 15, pipette tips sleeve 24 is moved It moves to some liquid-transfering gun 22 of setting, pipette tips lead screw stepper motor 29 rotates forward, and nut drives pipette tips sleeve fixed plate 26 It is moved down along sliding rail 2 28, liquid-transfering gun 22 is inserted in 24 bottom end of pipette tips sleeve, and then pipette tips lead screw stepper motor 29 inverts, pipette tips Sleeve 24 carries liquid-transfering gun 22 and moves up, and then X-axis motor driver 13 and Y-axis motor driver 15 act, and makes liquid-transfering gun 22 are moved in 24 orifice plates right above certain liquid storage hole, and then pipette tips lead screw stepper motor 29 rotates forward again, and pipette tips sleeve 24 drives Liquid-transfering gun 22 moves down, and liquid-transfering gun 22 stretches in 24 orifice plates in certain liquid storage hole, after extracting a certain amount of culture solution, pipette tips lead screw Stepper motor 29 inverts, and pipette tips sleeve 24 drives liquid-transfering gun 22 to move up, and then 38 interoperation of rotating stepper motor, prevents The extra culture solution in 22 bottom end of liquid-transfering gun arbitrarily drips；Then X-axis motor driver 13 and Y-axis motor driver 15 act, and match Co-clip grabs lead screw stepper motor 17 and electronic gripper 21 acts, and the sampling plug 52 of corresponding 44 top of tank body is taken out, then X-axis Motor driver 13 and Y-axis motor driver 15 act again, and cooperate rotating stepper motor 38 and pipette tips lead screw stepping electricity Culture solution in liquid-transfering gun 22 is injected into tank body 44 by the movement of machine 29, completion, is then controlled liquid-transfering gun 22 and is moved up, cooperates X The movement of axis motor driver 13 and Y-axis motor driver 15 completes this liquid-transfering gun 22 and moves back the disassembly unloaded in component 8 in pipette tips, Plug 52 will then be sampled in X-axis motor driver 13, Y-axis motor driver 15, gripper lead screw stepper motor 17 and electrical clip It grabs and is reset at sample tap again under 21 interoperation.

Claims (10)

1. a kind of automatic training station of parallel cell, it is characterised in that: including working station frame (1), working station frame (1) lower plate upper surface is set there are three region, and respectively parallel cell cultivation region, vessel rest area (2) and culture solution stores Tank rest area (3), working station frame (1) upper end are equipped with the liquid relief sampler (5) with these three region cooperatings；In parallel A number of parallel cell culture apparatus (4) is equipped in cell culture area.

2. the automatic training station of parallel cell according to claim 1, it is characterised in that: liquid relief sampler (5) packet X-axis moving assembly and Y-axis moving assembly are included, Y-axis moving assembly is fixedly connected with the sliding block of X-axis moving assembly, Y-axis moving assembly Sliding block be fixedly connected support transverse slat (14), support transverse slat (14) bottom surface be fixedly connected with supporting vertical plate (11), supporting vertical plate (11) surface is provided with pipette tips moving assembly, gripper moving assembly and sucker moving assembly.

3. the automatic training station of parallel cell according to claim 2, it is characterised in that: gripper moving assembly includes folder Lead screw stepper motor (17) and sliding rail one (18) are grabbed, the screw rod (19) of gripper lead screw stepper motor is parallel with sliding rail one (18) to be set It sets, connect electrical clip grasps fixed board to the sliding block one in the nut and sliding rail one (18) of gripper lead screw stepper motor (17) simultaneously (20), electrical clip grasps fixed board (20) bottom end and is provided with electronic gripper (21)；

Pipette tips moving assembly includes pipette tips lead screw stepper motor (29) and sliding rail two (28), the screw rod of pipette tips lead screw stepper motor (27) it is arranged in parallel with sliding rail two (28), the sliding block two in the nut and sliding rail two (28) of pipette tips lead screw stepper motor (29) is simultaneously It connects pipette tips sleeve fixed plate (26), pipette tips sleeve (24) is located in pipette tips sleeve by pipette tips sleeve briquetting (23) compression and fixes Plate (26) bottom end, pipette tips sleeve (24) top are equipped with threaded sleeve (25)；

Sucker moving assembly includes sucker lead screw stepper motor (30) and sliding rail three (32), the screw rod of sucker lead screw stepper motor (31) it is arranged in parallel with sliding rail three (32), the sliding block San Tong method in the nut and sliding rail three (32) of sucker lead screw stepper motor (30) Connecting sucker fixed plate (33), sucker fixed plate (33) side are equipped with sucker and stand side plate (34), and sucker founds side plate (34) bottom end company It connects sucker vacuum box (35), sucker vacuum box (35) bottom surface connects vacuum chuck (36), sucker vacuum box (35) upper surface connector Pass through the vacuum port of corrugated hose (39) connection minipump (41), the exhaust outlet joint noise reduction device of minipump (41) (40)。

4. the automatic training station of parallel cell according to claim 1, it is characterised in that: set in vessel rest area (2) There is vessel platform, vessel platform top is equipped with 24 orifice plate areas (10), pipette tips storage area (7) and centrifugation area under control (9), 24 orifice plate areas (10) one Side is equipped with 24 orifice plate plastic lid mounting tables (6), and pipette tips storage area (7) side is moved back equipped with pipette tips unloads component (8).

5. the automatic training station of parallel cell according to claim 1, it is characterised in that: parallel cell culture apparatus (4) include supporting frame (42), be provided with culture tank mixer drive and parallel bioreactor above supporting frame (42), Culture tank mixer drive includes driving box body (46), and driving box body (46) interior both ends are respectively equipped with motor side connecting shaft (56) With tank body side connecting shaft (81), motor side connecting shaft (56) and tank body side connecting shaft (81) are separately connected stirring motor (61) output Axis and the agitating paddle (75) in parallel cell culture tank.

6. the automatic training station of parallel cell according to claim 5, it is characterised in that: driving box body (46) includes upper Lid plate (53) and lower casing plate (58), upper box cover plate (53) and lower casing plate (58) composition driving box body (46) shell, driving The internal both ends of box body (46) are respectively provided with a synchronous pulley (54), are connected between two synchronous pulleys (54) by synchronous belt (94) It connects, two synchronous pulley (54) tops are embedded in upper box cover plate (53) top surface, and synchronous pulley (54) and upper box cover plate (53) Between be equipped with bearing one (55)；It is fixed with motor side connecting shaft (56) in the middle part of one of synchronous pulley (54), motor side connects Spindle (56) bottom passes through lower casing plate (58) bottom plate, by the output axis connection of shaft coupling and stirring motor (61), another Tank body side connecting shaft (81) is fixed in the middle part of synchronous pulley (54), tank body side connecting shaft (81) bottom passes through lower casing plate (58) Bottom plate is connect with agitating paddle (75) in culture tank；Motor side connecting shaft (56) and tank body side connecting shaft (81) are respectively under Bearing two (57) is equipped between lid plate (58).

7. the automatic training station of parallel cell according to claim 5, it is characterised in that: parallel cell culture tank includes Tank body (44), tank body (44) top are equipped with cover (79), and tank body (44) is fixed with cover (79) by compression mode, tank body (44) Interior to be equipped with agitating paddle (75), agitating paddle (75) spindle top runs through cover (79)；Feed supplement connector (48) are equipped with outside tank body (44) With inlet suction port (43), feed supplement connector (48) passes through cover (79) by feed supplement branch pipe (76) and is connected to tank body (44) inside, into Gas connector (43) is communicated in the respective line in cover (79) or in tank body (44) by external pipeline；Cover (79) is equipped with and takes Sample mouth is placed with sampling plug (52) engaged therewith in sample tap；Cover (79) is equipped with PH electrode interface (51)；Tank body (44) O-ring seal (88) are equipped between cover (79).

8. the automatic training station of parallel cell according to claim 5, it is characterised in that: stirring motor (61) is set to electricity In machine fixing seat (45), motor fixing seat (45) is fixedly connected with supporting frame (42), is equipped with simultaneously in motor fixing seat (45) Peltier cooler, peltier cooler top are equipped with condensation fixed plate (83), condense fixed plate (83) and lower casing plate (58) The bottom face contact of bottom plate；Cover (79) top surface of culture tank is close to setting with lower casing plate (58) bottom surface simultaneously；Semiconductor Cooler includes radiator fan (60), is equipped with cold sink (59) above radiator fan (60), cold sink (59) with it is cold Compressing between solidification fixed board (83) has cooling semiconductor (82), and cooling semiconductor (82) exterior circumferential is coated with heat insulating mattress (80), And lower casing plate (58) is aluminum.

9. the automatic training station of parallel cell according to claim 7, it is characterised in that: set above supporting frame (42) It sets gas-liquid block (50), lower feed supplement channel (72) channel He Paixi (71) is equipped with inside gas-liquid block (50), gas is run through in lower feed supplement channel Liquid block (50), row wash channel (71) top and are connected to by horizontal channel (73) with lower feed supplement channel (72) top, and row washes channel (71) bottom end outlet is set to gas-liquid block (50) bottom surface；Feed supplement connector (48) are placed on gas-liquid block (50) top, in feed supplement connector (48) Equipped with upper feed supplement channel (74), upper feed supplement channel (74) upper end connects feed supplement branch pipe (76), upper feed supplement channel (74) lower end connection Feed supplement tube head, feed supplement tube head outer wall are equipped with long sealing ring (84), and feed supplement tube head carries long sealing ring (84) and is inserted into lower feed supplement channel (72) in.

10. the automatic training station of parallel cell according to claim 7, it is characterised in that: the branch below tank body (44) Support body (42) is equipped with stop sleeve, and stop sleeve includes outer sleeve (62) and inner sleeve (63), and inner sleeve (63) is set to housing Cylinder (62) is internal, and cavity is equipped between inner sleeve (63) and outer sleeve (62), between outer sleeve (62) and inner sleeve (63) Partition (69) are set at cavity one, partition (69) two sides are respectively provided with a pagoda connector (70)；Two pagoda connector (70) one end It is respectively communicated with the cavity of partition (69) two sides, the other end is separately connected corresponding pipeline, the top of outer sleeve (62) and inner sleeve (63) End connection, outer sleeve (62) are fixedly connected with supporting frame (42).