CN209193952U - A kind of plant hybridization experiment PCR reaction unit convenient for adding catalyzing enzyme - Google Patents

A kind of plant hybridization experiment PCR reaction unit convenient for adding catalyzing enzyme Download PDF

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Publication number
CN209193952U
CN209193952U CN201821936951.XU CN201821936951U CN209193952U CN 209193952 U CN209193952 U CN 209193952U CN 201821936951 U CN201821936951 U CN 201821936951U CN 209193952 U CN209193952 U CN 209193952U
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CN
China
Prior art keywords
sample cell
main body
instrument main
thermovent
pcr reaction
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Expired - Fee Related
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CN201821936951.XU
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Chinese (zh)
Inventor
陈全战
张边江
唐宁
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Nanjing Xiaozhuang University
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Nanjing Xiaozhuang University
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Priority to CN201821936951.XU priority Critical patent/CN209193952U/en
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Abstract

The utility model discloses a kind of plant hybridization experiment PCR reaction units convenient for adding catalyzing enzyme, including PCR instrument main body and sample cell, operation panel is provided in front of the PCR instrument main body, the sample cell is located at the top of PCR instrument main body, and test tube hole is provided on the inside of sample cell, cover board is provided with above the sample cell, and fixation hole is provided on the inside of cover board, seal plug is provided on the inside of the fixation hole, it is provided with thermovent on the outside of the radiating fin, active insulation plate is provided on the outside of the thermovent.This is provided with seal plug with PCR reaction unit convenient for the plant hybridization experiment of addition catalyzing enzyme, better seal effect can be played in the use process of device, it prevents that test tube is poorly sealed and PCR reaction mixture reagent is caused to evaporate, catalyzing enzyme can be added using needle tubing by the groove of seal plug simultaneously, and seal plug can keep the sealing function to test tube.

Description

A kind of plant hybridization experiment PCR reaction unit convenient for adding catalyzing enzyme
Technical field
The utility model relates to field of biomedicine technology, specially a kind of plant hybridization experiment convenient for adding catalyzing enzyme With PCR reaction unit.
Background technique
PCR, that is, polymerase chain reaction, that is, refer to and carry out DNA replication dna in vitro, a small amount of DNA is cultivated Amplification needs the temperature was then adjusted to suitable condition during PCR reaction, carries out quick PCR by archaeal dna polymerase Reaction, existing PCR reaction experiment be usually operated by the way that sample to be put into test tube by PCR instrument, but such one Secondary property test tube causes poorly sealed it is easy to appear uneven thickness in use, and experiment reagent is caused to evaporate, and fixed Closing structure is not easy to the addition of catalyzing enzyme, while PCR instrument is existing by the process of heating and cooling in use The open type heat release hole of device will lead to device heating it is slower, enclosed structure will affect the radiating and cooling of device again, inconvenience It is quickly reused in device.
In view of the above-mentioned problems, carrying out innovative design on the basis of original PCR reaction unit.
Utility model content
The plant hybridization experiment that the purpose of this utility model is to provide a kind of convenient for adding catalyzing enzyme is reacted with PCR to be filled It sets, is not easy to the addition of catalyzing enzyme to test tube seal approach to solve to propose that PCR reaction unit lacks in above-mentioned background technique, It radiates after use relatively slow, is not easy to the problem of quickly using.
To achieve the above object, the utility model provides the following technical solutions: a kind of plant convenient for adding catalyzing enzyme is miscellaneous Experiment PCR reaction unit, including PCR instrument main body and sample cell are handed over, is provided with operation panel in front of the PCR instrument main body, And the side of operation panel is provided with display screen, the sample cell is located at the top of PCR instrument main body, and the inside setting of sample cell There is test tube hole, cover board is provided with above the sample cell, and be provided with fixation hole on the inside of cover board, the inside of the fixation hole It is provided with seal plug, and the lower section setting of seal plug is fluted, and tongue is provided with above seal plug, the sample It is provided with heating plate below product pond, and is provided with radiating fin on the outside of heating plate, is provided on the outside of the radiating fin Thermovent, and Air Filter is provided on the inside of thermovent, active insulation plate, and active insulation are provided on the outside of the thermovent Shifting block is provided on the outside of plate, and the side of active insulation plate is provided with loose slot.
Preferably, it is evenly distributed with test tube hole on the inside of the sample cell, and the central axes of test tube hole and PCR instrument main body Perpendicular bisector is parallel to each other.
Preferably, the rear side of the cover board is that hinge connects, and fixation is evenly distributed on the inside of cover board with PCR instrument main body Hole, and the position of fixation hole and test tube hole is corresponding.
Preferably, the seal plug, groove and tongue are integrated, and pass through between seal plug and fixation hole Tongue composition is connected together.
Preferably, the thermovent is located at the left and right sides of PCR instrument main body, and is provided between thermovent and sample cell scattered Hot fin.
Preferably, the structure of the active insulation plate and thermovent is coincide, and the outside of active insulation plate and PCR instrument main body Slidable structure is constituted by loose slot.
Compared with prior art, the utility model has the beneficial effects that should be convenient for the plant hybridization experiment of addition catalyzing enzyme With PCR reaction unit,
1, it is provided with seal plug corresponding with test tube, cooperates cover board to use in the use process of device, is had certain The seal plug of thickness can be bonded in cover plate closing with sample tube, play better seal effect by the fitting of groove Fruit prevents test tube poorly sealed and PCR reaction mixture reagent is caused to evaporate, while can be made by the groove of seal plug Catalyzing enzyme is added with needle tubing, and seal plug can keep the sealing function to test tube, improve the practicability of device;
2, it is provided with active insulation plate, shifting block and loose slot is cooperated to use in the use process of device, it can be in order to right Active insulation plate carries out activity adjustment, good heat insulating effect can be played during device is heated, simultaneously Active insulation plate can be removed after device use, cooperated radiating fin and thermovent to carry out fast cooling heat dissipation, improved The convenience that device uses.
Detailed description of the invention
Fig. 1 is the utility model side structure schematic diagram;
Fig. 2 is enlarged structure schematic diagram at a in the utility model Fig. 1;
Fig. 3 is the utility model positive structure schematic;
Fig. 4 is the utility model overlooking structure diagram.
In figure: 1, PCR instrument main body;2, operation panel;3, display screen;4, sample cell;5, test tube hole;6, cover board;7, fixed Hole;8, seal plug;9, groove;10, tongue;11, heating plate;12, radiating fin;13, thermovent;14, Air Filter;15, living Dynamic thermal insulation board;16, shifting block;17, loose slot.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work Every other embodiment obtained, fall within the protection scope of the utility model.
Please refer to Fig. 1-4, the utility model provides a kind of technical solution: a kind of plant hybridization convenient for adding catalyzing enzyme is real It tests with PCR reaction unit, including PCR instrument main body 1, operation panel 2, display screen 3, sample cell 4, test tube hole 5, cover board 6, fixation hole 7, seal plug 8, groove 9, tongue 10, heating plate 11, radiating fin 12, thermovent 13, Air Filter 14, active insulation plate 15, Shifting block 16 and loose slot 17, the front of PCR instrument main body 1 is provided with operation panel 2, and the side of operation panel 2 is provided with display Screen 3, sample cell 4 is located at the top of PCR instrument main body 1, and the inside of sample cell 4 is provided with test tube hole 5, and the top of sample cell 4 is set It is equipped with cover board 6, and the inside of cover board 6 is provided with fixation hole 7, the inside of fixation hole 7 is provided with seal plug 8, and seal plug 8 Lower section setting fluted 9, and the top of seal plug 8 is provided with tongue 10, and the lower section of sample cell 4 is provided with heating plate 11, and the outside of heating plate 11 is provided with radiating fin 12, the outside of radiating fin 12 is provided with thermovent 13, and thermovent 13 Inside be provided with Air Filter 14, the outside of thermovent 13 is provided with active insulation plate 15, and the outside of active insulation plate 15 is set It is equipped with shifting block 16, and the side of active insulation plate 15 is provided with loose slot 17.
The inside of the sample cell 4 of this example is evenly distributed with test tube hole 5, and the central axes of test tube hole 5 and PCR instrument main body 1 Perpendicular bisector is parallel to each other, convenient for carrying out placement fixation to test tube by test tube hole 5.
The rear side of cover board 6 is connected with PCR instrument main body 1 for hinge, and the inside of cover board 6 is evenly distributed with fixation hole 7, and The position of fixation hole 7 and test tube hole 5 is corresponding, convenient for passing through the test tube sample in the closed pair seal plug 8 and test tube hole 5 of cover board 6 Product are fixed.
Seal plug 8, groove 9 and tongue 10 are integrated, and pass through tongue between seal plug 8 and fixation hole 7 10 constitute and are connected together, convenient for further being sealed to sample tube by dismountable seal plug 8, while can be must When wanting, by needle tubing pass through seal plug 8 add catalyzing enzyme, and the seal plug 8 of medical grade rubber material can remain compared with Good sealing effect.
Thermovent 13 is located at the left and right sides of PCR instrument main body 1, and is provided with radiating fin between thermovent 13 and sample cell 4 Piece 12, convenient for carrying out rapid cooling to device after device use, convenient for the taking-up of test tube and the quick use of device.
The structure of active insulation plate 15 and thermovent 13 is coincide, and the outside of active insulation plate 15 and PCR instrument main body 1 passes through Loose slot 17 constitutes slidable structure, movable in the heating process of device convenient for carrying out mobile adjusting to active insulation plate 15 Thermal insulation board 15 can play good function of heat insulation, and active insulation plate 15 can also be removed to progress after device use Rapid cooling.
Working principle:, first will dress when using PCR reaction unit using this convenient for the plant hybridization experiment of addition catalyzing enzyme The power supply and power grid set are connected with each other, and according to FIG. 1 and FIG. 3, can be rotated cover board 6 first in the use process of device It opens, then sample tube is placed into the test tube hole 5 of sample cell 4, closes cover board 6 later, so that the seal plug under cover board 6 First 8, by covering on sample tube at groove 9, are sealed sample tube, keep active insulation plate 15 to be located at heat dissipation later 13 outside of mouth can start to carry out device heating progress PCR reaction.
When carrying out PCR reaction, according to Fig. 1-4, it is heat-insulated that active insulation plate 15 can play closing to thermovent 13 Effect, convenient for device control temperature raising can also be if necessary directly by using needle in the use process of device Tongue 10 of the pipe on seal plug 8 adds suitable catalyzing enzyme into sample tube, can be with after device use Directly seal plug 8 is taken out from fixation hole 7, convenient for keeping carrying out the sealing state of test tube after device use When cooling, shifting block 16 can be stirred, so that active insulation plate 15 is moved down along loose slot 17, leaks out the thermovent 13 at rear, Convenient for carrying out rapid cooling cooling to device by thermovent 13 and radiating fin 12, it is not described in detail in this specification interior Appearance belongs to the prior art well known to professional and technical personnel in the field.
While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art, It is understood that these embodiments can be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaired Change, replacement and variant, the scope of the utility model is defined by the appended claims and the equivalents thereof.

Claims (6)

1. a kind of plant hybridization experiment PCR reaction unit, including PCR instrument main body (1) and sample cell convenient for adding catalyzing enzyme (4), it is characterised in that: be provided with operation panel (2) in front of the PCR instrument main body (1), and the side of operation panel (2) is set It is equipped with display screen (3), the sample cell (4) is located at the top of PCR instrument main body (1), and is provided with test tube on the inside of sample cell (4) Hole (5) is provided with cover board (6) above the sample cell (4), and is provided with fixation hole (7) on the inside of cover board (6), described solid Determine to be provided on the inside of hole (7) seal plug (8), and fluted (9) are arranged in the lower section of seal plug (8), and seal plug (8) it is provided with above tongue (10), is provided with heating plate (11) below the sample cell (4), and heating plate (11) is outer Side is provided with radiating fin (12), is provided with thermovent (13) on the outside of the radiating fin (12), and thermovent (13) is interior Side is provided with Air Filter (14), is provided with active insulation plate (15) on the outside of the thermovent (13), and active insulation plate (15) Outside be provided with shifting block (16), and the side of active insulation plate (15) is provided with loose slot (17).
2. a kind of plant hybridization experiment PCR reaction unit convenient for adding catalyzing enzyme according to claim 1, feature It is: is evenly distributed with test tube hole (5) on the inside of the sample cell (4), and the central axes of test tube hole (5) and PCR instrument main body (1) Perpendicular bisector be parallel to each other.
3. a kind of plant hybridization experiment PCR reaction unit convenient for adding catalyzing enzyme according to claim 1, feature Be: the rear side of the cover board (6) is that hinge connects, and is evenly distributed with fixation on the inside of cover board (6) with PCR instrument main body (1) Hole (7), and fixation hole (7) is corresponding with the position of test tube hole (5).
4. a kind of plant hybridization experiment PCR reaction unit convenient for adding catalyzing enzyme according to claim 1, feature Be: the seal plug (8), groove (9) and tongue (10) are integrated, and seal plug (8) and fixation hole (7) it Between by tongue (10) constitute be connected together.
5. a kind of plant hybridization experiment PCR reaction unit convenient for adding catalyzing enzyme according to claim 1, feature Be: the thermovent (13) is located at the left and right sides of PCR instrument main body (1), and is arranged between thermovent (13) and sample cell (4) There are radiating fin (12).
6. a kind of plant hybridization experiment PCR reaction unit convenient for adding catalyzing enzyme according to claim 1, feature Be: the structure of the active insulation plate (15) and thermovent (13) is coincide, and active insulation plate (15) and PCR instrument main body (1) Outside by loose slot (17) constitute slidable structure.
CN201821936951.XU 2018-11-23 2018-11-23 A kind of plant hybridization experiment PCR reaction unit convenient for adding catalyzing enzyme Expired - Fee Related CN209193952U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201821936951.XU CN209193952U (en) 2018-11-23 2018-11-23 A kind of plant hybridization experiment PCR reaction unit convenient for adding catalyzing enzyme

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201821936951.XU CN209193952U (en) 2018-11-23 2018-11-23 A kind of plant hybridization experiment PCR reaction unit convenient for adding catalyzing enzyme

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CN209193952U true CN209193952U (en) 2019-08-02

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110408535A (en) * 2019-08-15 2019-11-05 淮阴师范学院 A kind of breeding apparatus can be improved activity of beta-glucosidase

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110408535A (en) * 2019-08-15 2019-11-05 淮阴师范学院 A kind of breeding apparatus can be improved activity of beta-glucosidase

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Granted publication date: 20190802

Termination date: 20201123