CN212894767U

CN212894767U - Modularized automatic amplification culture equipment for personalized customized stem cells

Info

Publication number: CN212894767U
Application number: CN202020977445.6U
Authority: CN
Inventors: 不公告发明人
Original assignee: Individual
Current assignee: Shaanxi Mingyang Deshun Biotechnology Co ltd
Priority date: 2020-06-02
Filing date: 2020-06-02
Publication date: 2021-04-06
Anticipated expiration: 2030-06-02

Abstract

The utility model discloses an individualized and customized stem cell automatic amplification culture equipment based on modularization, which comprises a box body, wherein both sides of the bottom of the box body are provided with a taking and placing device, the top end of a slide block in the taking and placing device is fixedly connected with the bottom surfaces of both sides of a placing table, the upper end of the placing table is provided with a fixing device, the bottom of a leak hole placing groove in the fixing device is provided with a culture box, the individualized and customized stem cell automatic amplification culture equipment based on modularization has simple structure and complete functions, the culture temperature, the humidity and the carbon dioxide concentration can be regulated and controlled in real time, great convenience is brought to the use of a user, the rapid and stable adjustment of the stem cell culture equipment can be realized through the taking and placing device and the fixing device according to the use requirement, the efficient and appropriate adjustment of the stem cell culture environment can be realized through a temperature regulating device and a material adding and taking device, the culture quality of the stem cells is ensured, and the culture efficiency of the stem cells is improved.

Description

Modularized automatic amplification culture equipment for personalized customized stem cells

Technical Field

The utility model relates to a stem cell culture equipment technical field specifically is an automatic amplification culture equipment of individualized customization stem cell based on modularization.

Background

The stem cell is a pluripotent cell with self-replicating capacity, can be differentiated into various functional cells under specific conditions, is called as a universal cell in the medical field, is particularly important in treating hereditary diseases and malignant tumors and culturing tissues and organs, is often cultured in a specific environment by auxiliary equipment in the culture process of the stem cell, is excessively complex in structure and single in function, cannot regulate and control culture temperature, humidity and carbon dioxide concentration in real time, brings great troubles to the use of a user, cannot realize quick and stable taking and placing of stem cell culture equipment according to the use requirement, cannot realize efficient and appropriate regulation of the stem cell culture environment, invisibly increases the workload of personnel, cannot guarantee the culture quality of the stem cell, and causes the culture efficiency of the stem cell, therefore, the realization of the modularized personalized stem cell automatic amplification culture equipment capable of solving the problems is imperative.

SUMMERY OF THE UTILITY MODEL

The to-be-solved technical problem of the utility model is overcome current defect, the utility model provides an automatic expansion culture equipment of individualized customization stem cell based on modularization, moreover, the steam generator is simple in structure, it is multiple functional, to cultivateing the temperature, humidity and carbon dioxide concentration regulate and control in real time, very big facility has been brought to user's use, and can be according to the user demand, through getting fast and stable getting of putting device and fixing device realization to stem cell culture equipment and put, realize the high-efficient suitable regulation to the stem cell culture environment through temperature regulation apparatus and feeding device, personnel's work burden has been alleviateed, the stem cell culture quality has been guaranteed, stem cell's cultivation efficiency has been promoted, can effectively solve the problem in the background art.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides an automatic propagation culture apparatus of individualized customization stem cell based on modularization, includes the box, the bottom of the case both sides of box are equipped with gets puts the device, get the top of putting the device in the slider all with the both sides bottom surface fixed connection of putting the platform, the upper end of putting the platform is equipped with fixing device, the tank bottom that the groove was put to the small opening in fixing device is equipped with the incubator, the left side opening part of box is equipped with temperature regulation apparatus, the upper end right side of box is equipped with the feeding device, the upper end left side of box is equipped with the carbon dioxide case, and the gas outlet department of carbon dioxide case is equipped with the valve pipe fitting, and the end of valve pipe fitting passes the upper end through-hole of box and corresponds the setting with the upper end opening of incubator, the right side of box is equipped with the humidifier, and the exit of humidifier is equipped with the damp pipe, and the end of damp, Humidity transducer and carbon dioxide sensor, the front side opening one side of box articulates there is the shutter, the upper end front side of box is equipped with display, battery and PLC controller from the left hand right side in proper order, the output of battery is connected to the input electricity of PLC controller, the input of PLC controller is connected to temperature transducer, humidity transducer and carbon dioxide sensor's the equal electricity of output, the output of PLC controller is connected to the equal electricity of humidifier and the equal electricity of display input.

As a preferred technical scheme of the utility model, it includes the spout track to get the device, the orbital bottom surface of spout all with the both sides incasement bottom surface fixed connection of box, the orbital equal sliding connection in inslot of spout has the slider, the back wall of box is equipped with electronic flexible post, the top of electronic flexible post and the rear side fixed connection of putting the platform, the output of PLC controller is connected to electronic flexible post's input electricity.

As the utility model discloses a preferred technical scheme, fixing device includes that the leak puts the groove, the upper end middle part of putting the platform is equipped with the leak and puts the groove, and the leak all is equipped with the spring in putting the inslot wall mounting groove of groove, and the top of spring all is equipped with the fixed plate.

As a preferred technical scheme of the utility model, temperature regulation apparatus is including the installation cover, the left side opening part of box is equipped with the installation cover, and the bottom is equipped with the heating plate in the cover of installation cover, be equipped with the fan on the left side wall in the cover of installation cover, the lateral wall of box is the cavity, and the inside wall of box is covered with the air vent, the upper end opening part of installation cover is equipped with dustproof ventilative net, the output of PLC controller is connected to the equal electricity of input of heating plate and fan.

As a preferred technical scheme of the utility model, add the material device and include the injection chamber, fixed surface is connected on the bottom surface of injection chamber and the right side of box, the inside sliding connection of injection chamber has the push pedal, the upper end opening part of injection chamber is equipped with the filler tube, and the surface of filler tube is equipped with the valve, the discharge gate department of injection chamber is equipped with the inlet pipe, and the upper end through-hole that the box was passed to the bottom of inlet pipe corresponds the setting with the upper end opening of incubator, the upper end right side of box is equipped with the support, and the upper end of support is equipped with electric telescopic handle, electric telescopic handle's top and the side fixed connection of push pedal, the output of PLC controller is connected to electric telescopic handle's input.

Compared with the prior art, the beneficial effects of the utility model are that: the modularized automatic stem cell amplification culture equipment based on individuation customization is simple in structure, convenient control of the device is realized through regulation and control of a PLC, multiple functions are achieved, culture temperature, humidity and carbon dioxide concentration are regulated and controlled in real time, great convenience is brought to use of a user, the electric telescopic column can stretch out and draw back through the taking and placing device according to use requirements, due to sliding connection of a sliding block on a sliding groove rail, the fixing device is used for elastically supporting a fixing plate through a spring, so that side fixing of an incubator is realized, rapid and stable taking and placing of the stem cell culture equipment are achieved, the fan rotates through the temperature regulating device, circulation of gas inside and outside the incubator is accelerated through air holes, cooling inside the incubator is realized, gas is heated through a heating plate, temperature rise inside the incubator is improved, the telescopic rod is electrically stretched through the feeding device, because the sliding connection of push pedal in the injection chamber, and then add quantitative culture solution through the inlet pipe in to the incubator, reach the high-efficient suitable regulation to the stem cell culture environment, alleviateed personnel's work burden, guaranteed stem cell culture quality, promoted stem cell's cultivation efficiency.

Drawings

FIG. 1 is a front view of the structure of the present invention;

FIG. 2 is a schematic sectional view of the inside of the structural box body of the present invention;

FIG. 3 is a schematic view of the internal cross-section of the structure taking and placing device of the present invention;

FIG. 4 is a schematic cross-sectional view of the inside of the structure temperature adjusting device of the present invention;

fig. 5 is the internal cross-sectional view of the structure feeding device of the present invention.

In the figure: the device comprises a box body 1, a taking and placing device 2, a chute track 21, a slide block 22, an electric telescopic column 23, a placing table 3, a fixing device 4, a leak hole placing groove 41, a spring 42, a fixing plate 43, an incubator 5, a temperature adjusting device 6, a mounting cover 61, a heating plate 62, a fan 63, an air vent 64, a dustproof and breathable net 65, a material adding device 7, an injection cavity 71, a push plate 72, a filling pipe 73, a valve 74, a feeding pipe 75, a support 76, an electric telescopic rod 77, a carbon dioxide box 8, a valve pipe 9, a humidifier 10, a wet gas pipe 11, a temperature sensor 12, a humidity sensor 13, a carbon dioxide concentration sensor 14, a door 15, a display 16, a storage battery 17 and a PLC (programmable logic controller).

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-5, the present invention provides a technical solution: a modularized automatic amplification culture device for personalized stem cells comprises a box body 1, wherein the box body 1 is used for providing a supporting and placing place for internal devices and providing a natural barrier for stem cell culture, two sides of the bottom of the box body 1 are provided with a taking and placing device 2, the taking and placing device 2 comprises a chute rail 21, the chute rail 21 is used for providing sliding support for a sliding block 22, the bottom surface of the chute rail 21 is fixedly connected with the bottom surfaces of the box bodies 1 at two sides, the sliding block 22 is slidably connected with the inside of the chute rail 21, the rear wall of the box body 1 is provided with an electric telescopic column 23, the electric telescopic column 23 is telescopic, the sliding block 22 is slidably connected with the chute rail 21 so as to achieve the purpose of quickly taking and placing stem cell culture devices, the top end of the electric telescopic column 23 is fixedly connected with the rear side of a placing table 3, and the top end of the sliding block 22 in the taking and placing device 2 is, the upper end of the placing table 3 is provided with a fixing device 4, the fixing device 4 comprises a leak hole placing groove 41, the leak hole placing groove 41 realizes placing of the incubator 5, the upper middle part of the placing table 3 is provided with the leak hole placing groove 41, springs 42 are arranged in groove mounting grooves of the inner side wall of the leak hole placing groove 41, the top ends of the springs 42 are respectively provided with a fixing plate 43, under the action of the springs 42 elastically supporting the fixing plates 43, the side fixing of the incubator 5 is realized, the springs 42 elastically support the fixing plates 43, the side fixing of the incubator 5 is realized, the incubator 5 provides a storage place for culturing the stem cells, the bottom of the leak hole placing groove 41 in the fixing device 4 is provided with the incubator 5, the left side opening of the box body 1 is provided with a temperature adjusting device 6, the temperature adjusting device 6 comprises a mounting cover 61, the mounting cover 61 provides a placing space for the internal device, the left side opening of the box body 1, the heating sheet 62 is arranged at the inner bottom end of the cover of the mounting cover 61, the fan 63 is arranged on the left side wall in the cover of the mounting cover 61, the side wall of the box body 1 is a cavity, the inner side wall of the box body 1 is fully distributed with vent holes 64 to ensure rapid temperature rise and drop in the box body 1, the dustproof breathable net 65 is arranged at the upper end opening of the mounting cover 61, the dustproof breathable net 65 ensures the circulation of air inside and outside the box body 1, the fan 63 rotates to accelerate the circulation of air inside and outside the box body 1 through the vent holes 64, so that the temperature drop in the box body 1 is realized, the heating sheet 62 heats the air to improve the temperature rise in the box body 1, the right side of the upper end of the box body 1 is provided with the feeding device 7, the feeding device 7 comprises an injection cavity 71, the bottom surface of the injection cavity 71 is fixedly connected with the upper surface of the right side of the box body 1, the push plate 72 is, the surface of the filling pipe 73 is provided with a valve 74, a feed pipe 75 is arranged at a discharge port of the injection cavity 71, the feed pipe 75 realizes that materials such as culture solution and the like are injected into the incubator 5, the bottom end of the feed pipe 75 penetrates through a through hole at the upper end of the incubator 1 and is arranged corresponding to an opening at the upper end of the incubator 5, a bracket 76 is arranged at the right side of the upper end of the incubator 1 and provides support for an electric telescopic rod 77, the upper end of the bracket 76 is provided with the electric telescopic rod 77, the top end of the electric telescopic rod 77 is fixedly connected with the side surface of a push plate 72, the electric telescopic rod 77 extends, due to the sliding connection of the push plate 72 in the injection cavity 71, quantitative culture solution is added into the incubator 5 through the feed pipe 75, the left side of the upper end of the incubator 1 is provided with a carbon dioxide box 8, a valve pipe 9 is arranged at an air outlet, the upper end valve of the valve pipe fitting 9 is opened, carbon dioxide is injected into the box body 1 through the carbon dioxide box 8 until the standard is reached, the right side of the box body 1 is provided with a humidifier 10, the outlet of the humidifier 10 is provided with a wet air pipe 11, the humidifier 10 operates, the box body 1 is humidified through the wet air pipe 11, the end of the wet air pipe 11 penetrates through the inner wall of the right side box of the box body 1, the upper end of the placing table 3 is sequentially provided with a temperature sensor 12, a humidity sensor 13 and a carbon dioxide concentration sensor 14 from left to right, one side of the front side opening of the box body 1 is hinged with a stop door 15, the front side of the upper end of the box body 1 is sequentially provided with a display 16, a storage battery 17 and a PLC (programmable logic controller) 18 from left to right, the temperature sensor 12, the humidity sensor 13 and the, the input end of the PLC 18 is electrically connected with the output end of the storage battery 17 through the regulation and control of the PLC 18, the output ends of the temperature sensor 12, the humidity sensor 13 and the carbon dioxide concentration sensor 14 are electrically connected with the input end of the PLC 18, the input ends of the humidifier 10, the display 16, the electric telescopic column 23, the heating sheet 62, the fan 63 and the electric telescopic rod 77 are electrically connected with the output end of the PLC 18, the modes of the PLC 18 for controlling the humidifier 10, the display 16, the electric telescopic column 23, the heating sheet 62, the fan 63 and the electric telescopic rod 77 are all common methods in the prior art, humidifier 10, temperature sensor 12, humidity sensor 13, carbon dioxide concentration sensor 14, display 16, battery 17, electric telescopic column 23, heating plate 62, fan 63 and electric telescopic rod 77 are all the common elements of stem cell culture equipment in the prior art.

When in use: the baffle door 15 is opened, the culture box 5 with the prepared culture solution and raw materials is placed on the leak hole placing groove 41, the fixing plate 43 is fixed on the side surface of the culture box 5 under the elastic support of the spring 42 as the slide block 22 is in sliding connection on the chute track 21 to push out the placing table 3, the culture box 5 with the prepared culture solution and raw materials is placed on the leak hole placing groove 41, the electric telescopic column 23 contracts, the culture box 5 is taken in the box body 1, the baffle door 15 is closed, the temperature sensor 12, the humidity sensor 13 and the carbon dioxide concentration sensor 14 monitor the ambient temperature, the humidity and the carbon dioxide concentration of the culture box 5 in the box body 1 in real time, the storage battery 17 collects detection data, the temperature is regulated and controlled by the PLC 18, when the temperature needs to be regulated, the fan 63 rotates to accelerate the circulation of gas inside and outside the box body 1 through the air holes 64, the temperature reduction in the box body 1, improve temperature rise in the box 1, open the upper end valve of valve pipe fitting 9, inject carbon dioxide until the standard in with the box 1 through carbon dioxide case 8, humidifier 10 moves, through wet gas pipe 11 with box 1 humidification operation, through with quantitative culture solution through in the filler pipe 73 injects injection chamber 71, close valve 74, electric telescopic handle 77 extends, because the sliding connection of push pedal 72 in injection chamber 71, and then add quantitative culture solution in to incubator 5 through inlet pipe 75, the back of finishing cultivateing, take out incubator 5, each device disinfection washs and resumes the former state can.

The utility model has simple structure, the utility model realizes convenient control of the device by the regulation and control of the PLC 18, has complete functions, realizes real-time regulation and control of culture temperature, humidity and carbon dioxide concentration, brings great convenience for the use of users, and can realize the rapid and stable taking and placing of dry cell culture equipment by taking and placing the device 2 according to the use requirements, because the sliding connection of the slide block 22 on the chute track 21, the fixing device 4 is used for elastically supporting the fixing plate 43 by the spring 42, further realizing the side fixing of the incubator 5, the circulation of the gas inside and outside the box body 1 is accelerated by the air holes 64 by the rotation of the fan 63 in the temperature regulating device 6, the cooling in the box body 1 is realized, the heating sheets 62 heat the gas, the temperature rise in the box body 1 is improved, and the electric telescopic rod 77 is extended by the feeding device 7, because the sliding connection of push plate 72 in injection chamber 71, and then add quantitative culture solution through inlet pipe 75 in to incubator 5, reach the high-efficient suitable regulation to the stem cell culture environment, alleviateed personnel's work burden, guaranteed stem cell culture quality, promoted stem cell's cultivation efficiency.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A modularization-based automatic expansion culture equipment for personalized stem cells comprises a box body (1), and is characterized in that: the device is characterized in that the two sides of the bottom of the box body (1) are provided with a taking and placing device (2), the top ends of sliding blocks (22) in the taking and placing device (2) are fixedly connected with the bottom surfaces of the two sides of a placing table (3), the upper end of the placing table (3) is provided with a fixing device (4), the bottom of a leak hole placing groove (41) in the fixing device (4) is provided with an incubator (5), the left opening of the box body (1) is provided with a temperature regulating device (6), the right side of the upper end of the box body (1) is provided with a feeding device (7), the left side of the upper end of the box body (1) is provided with a carbon dioxide box (8), the air outlet of the carbon dioxide box (8) is provided with a valve pipe fitting (9), the end of the valve pipe fitting (9) penetrates through a through hole in the upper end of the box body (1) and is arranged corresponding to the upper opening of the, the end of wet pipe (11) runs through the right side incasement wall of box (1), the upper end of putting platform (3) is equipped with temperature sensor (12), humidity transducer (13) and carbon dioxide consistency transmitter (14) from the left hand right side in proper order, the front side opening one side of box (1) articulates there is shutter (15), the upper end front side of box (1) is equipped with display (16), battery (17) and PLC controller (18) from the left hand right side in proper order, the output of battery (17) is connected to the input electricity of PLC controller (18), the input of PLC controller (18) is connected to the equal electricity of output of temperature sensor (12), humidity transducer (13) and carbon dioxide consistency transmitter (14), the output of PLC controller (18) is connected to the equal electricity of input of humidifier (10) and display (16).

2. The device for automatically expanding and culturing the customized stem cells based on modularization as claimed in claim 1, wherein: get and put device (2) and include spout track (21), the bottom surface of spout track (21) all with the both sides incasement bottom surface fixed connection of box (1), the equal sliding connection in groove of spout track (21) has slider (22), the back wall of box (1) is equipped with electronic flexible post (23), the top of electronic flexible post (23) and the rear side fixed connection of putting platform (3), the output of PLC controller (18) is connected to the input electricity of electronic flexible post (23).

3. The device for automatically expanding and culturing the customized stem cells based on modularization as claimed in claim 1, wherein: the fixing device (4) comprises a leak hole placing groove (41), the upper end middle part of the placing table (3) is provided with the leak hole placing groove (41), springs (42) are arranged in groove inner side wall mounting grooves of the leak hole placing groove (41), and fixing plates (43) are arranged at the top ends of the springs (42).

4. The device for automatically expanding and culturing the customized stem cells based on modularization as claimed in claim 1, wherein: temperature regulation apparatus (6) are including installation cover (61), the left side opening part of box (1) is equipped with installation cover (61), and the bottom is equipped with heating plate (62) in the cover of installation cover (61), be equipped with fan (63) on the cover of installation cover (61) on the left side wall, the lateral wall of box (1) is the cavity, and the inside wall of box (1) is covered with air vent (64), the upper end opening part of installation cover (61) is equipped with dustproof ventilative net (65), the output of PLC controller (18) is all connected to the input electricity of heating plate (62) and fan (63).

5. The device for automatically expanding and culturing the customized stem cells based on modularization as claimed in claim 1, wherein: the feeding device (7) comprises an injection cavity (71), the bottom surface of the injection cavity (71) is fixedly connected with the upper surface of the right side of the box body (1), a push plate (72) is connected in the injection cavity (71) in a sliding manner, a filling pipe (73) is arranged at an opening at the upper end of the injection cavity (71), a valve (74) is arranged on the surface of the filling pipe (73), a feed pipe (75) is arranged at the discharge port of the injection cavity (71), the bottom end of the feed pipe (75) penetrates through the upper through hole of the box body (1) and is arranged corresponding to the upper opening of the incubator (5), a bracket (76) is arranged on the right side of the upper end of the box body (1), an electric telescopic rod (77) is arranged on the upper end of the bracket (76), the top end of the electric telescopic rod (77) is fixedly connected with the side surface of the push plate (72), the input end of the electric telescopic rod (77) is electrically connected with the output end of the PLC (18).