CN209167676U - A kind of microscope example fixed frame - Google Patents
A kind of microscope example fixed frame Download PDFInfo
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- CN209167676U CN209167676U CN201822219995.7U CN201822219995U CN209167676U CN 209167676 U CN209167676 U CN 209167676U CN 201822219995 U CN201822219995 U CN 201822219995U CN 209167676 U CN209167676 U CN 209167676U
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Abstract
A kind of microscope example fixed frame comprising bracket and rack-mount fixing piece, the bracket include support board, and the fixing piece and support board are by magnetic attraction mode secure fit, and the support board is iron plate, then fixing piece is magnetic nail；The support board is equipped with the first groove, the first magnet is equipped in first groove, then fixing piece is iron nail or magnetic nail or iron plate, the utility model improves the fixed stability of sample in the way of magnetic attraction, and sample image quality is not influenced, which is simple and convenient to operate, is reliable and practical.
Description
Technical field
The utility model relates to a kind of microscope example technique for fixing more particularly to a kind of microscope example fixed frames.
Background technique
Due to the intrinsic three-dimensional character of biological tissue so that the research and discovery of life science, such as to brain neuroblastoma projection,
The research such as vascular distribution and tumor microenvironment need to be furtherd investigate based on biological tissue's three-dimensional spatial information.Traditional
The demand of researcher is had been unable to meet to the research compared with thin-sliced samples or a small amount of cell, corresponding conventional imaging method is such as total
Focusing microscope has also no longer been applicable in sample three-dimensional imaging.
In recent years, for large scale biological sample (Mouse Whole Brain and spinal cord etc.) processing method and three-dimensional microscopy side
Method is gradually risen.Researcher uses CUBIC, CLARITY, BABB, a variety of transparence sides such as uDISCO/iDISCO, PEGASOS
Method carries out transparency process to different types of large scale biological sample, then with novel microscopes such as mating plate illumination microscopes
It is quickly obtained the 3-D image of not sliced complete large scale biological sample.
Sample is fixed on microscopical objective table and is imaged after transparency process, in imaging process, needs to guarantee
The stability of sample.Section sample is fixed between glass slide and coverslip before traditional co-focusing imaging, ensure that sample
Stability.However, the slide fixing means cannot achieve for large scale biological tissue samples.Currently, raw for large scale
The fixing means existing research of object sample, if Zeiss (Zeiss) Z1 microscope uses Agarose embedding sample, embedded samples
Agarose sucks imaging in glass tube, and this method operation is sufficiently complex, and in imaging process, and light passes through glass tube and agarose
Sample can be reached, the take up an official post medium of what different refractivity of optical path exists and can all influence to be ultimately imaged effect.German LaVision
Two generation mating plates illumination microscope (UltraMicroscope II) supports sample by the way of four fulcrums, and this method is very simple
Single but extremely unstable, when sample stage is in decline, no pressure above sample, sample easily floats in imaging liquid, especially right
The sample of CUBIC processing is very unfavorable, because the sample of water-soluble transparence method processing can be very soft, it is more difficult to rely on four
Fulcrum support sample is unfavorable for sample imaging.
Utility model content
The purpose of the utility model is to provide a kind of microscope example fixed frame, ensure that different shape, size it is transparent
Changing large sample can be stably fixed on microscope carrier, and the sample holder structure is simple, and fixing means is easy to operate, can
It is widely used in microscope imaging field.
The utility model technical solution used for the above purpose is:
A kind of microscope example fixed frame, including bracket and rack-mount fixing piece, the bracket includes loading
Plate, the fixing piece and the support board are by magnetic attraction mode secure fit.
Preferably, the bracket further includes transverse slat and riser, the riser both ends respectively with the transverse slat and the loading
Plate connection, the transverse slat are connect with three-dimensional mobile platform, and the support board is placed in imaging storehouse.
Preferably, several first grooves are offered on the support board, and the first magnet, institute are fixed in first groove
The number, shape and position distribution for stating the first magnet are identical as the first groove, the fixing piece be iron nail or magnetic nail or
Iron plate.
Preferably, the support board is iron plate, and the fixing piece is magnetic nail.
Preferably, the transverse slat lower plane offers several second grooves, is fixed with the second magnet in second groove,
The transverse slat is connect with the three-dimensional mobile platform by magnetic attraction mode.
The utility model has the beneficial effects that
1. the structure of the utility model microscope example fixed frame is simple, reliable and practical and easy to operate, can satisfy micro-
Mirror to transparence large sample imaging requirements, meanwhile, it is poor to transparence large sample stability maintenance in microscope easily to solve
The problem of, and the sample holder can ensure that light directly reaches on the sample of transparency process, further ensure image quality；
2. the utility model, by iron and magnetic, the magnetic attraction mode between magnetic and magnetic realizes that sample is fixed, this method spirit
Living, magnetic action is strong, can fully ensure that the stability of sample in imaging process.
3. fixing piece is using iron nail or iron plate or magnetic nail in the utility model, wherein iron nail or magnetic follow closely puncture sample,
It is easy to operate；Meanwhile the quality of iron is softer, be easy to be fabricated to the matched clamping shape of sample topography, to adapt to different samples
It is fixed, and fixing piece can first use, be also used multiple times, meanwhile, cost is relatively low for iron plate.
Detailed description of the invention
It, below will be right in order to illustrate more clearly of specific embodiment of the present invention or technical solution in the prior art
Specific embodiment or attached drawing needed to be used in the description of the prior art are briefly described, it should be apparent that, it is described below
In attached drawing be that some embodiments of the utility model are not paying creativeness for those of ordinary skill in the art
Under the premise of labour, it is also possible to obtain other drawings based on these drawings.
Fig. 1 is the structural schematic diagram of the fixed Samples EXAMPLE 1 of the utility model microscope example fixed frame；
Fig. 2 is the structural schematic diagram of the fixed Samples EXAMPLE 2 of the utility model microscope example fixed frame；
Fig. 3 is the structural schematic diagram of the fixed Samples EXAMPLE 3 of the utility model microscope example fixed frame；
Fig. 4 is the structural schematic diagram of the fixed Samples EXAMPLE 4 of the utility model microscope example fixed frame；
Fig. 5 is the structural schematic diagram of the fixed Samples EXAMPLE 5 of the utility model microscope example fixed frame；
Fig. 6 is the structural schematic diagram of the fixed Samples EXAMPLE 6 of the utility model microscope example fixed frame；
Structural schematic diagram below the utility model microscope example fixed frame transverse slat of the position Fig. 7；
In figure: 1- bracket；101- transverse slat；102- riser；103- support board；2- fixing piece；The first groove of 3-；301- first
Magnet；The second groove of 4-；The second magnet of 401-；.
Specific embodiment
It is described in detail below in conjunction with embodiment of the attached drawing to technical solutions of the utility model.Following embodiment is only
For clearly illustrating the technical solution of the utility model, therefore it is only used as example, and it is originally practical to cannot be used as a limitation limitation
Novel protection scope.
It should be noted that unless otherwise indicated, technical term or scientific term used in this application should be this reality
The ordinary meaning understood with novel one of ordinary skill in the art.
In the description of the present application, it is to be understood that term " center ", " longitudinal direction ", " transverse direction ", " length ", " width ",
" thickness ", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom" "inner", "outside", " up time
The orientation or positional relationship of the instructions such as needle ", " counterclockwise ", " axial direction ", " radial direction ", " circumferential direction " be orientation based on the figure or
Positional relationship is merely for convenience of describing the present invention and simplifying the description, rather than the device or member of indication or suggestion meaning
Part must have a particular orientation, be constructed and operated in a specific orientation, therefore should not be understood as limiting the present invention.
In addition, term " first ", " second " etc. are used for description purposes only, it is not understood to indicate or imply relatively important
Property or implicitly indicate the quantity of indicated technical characteristic.The meaning of " plurality " is two in the description of the present invention,
More than, unless otherwise specifically defined.
In this application unless specifically defined or limited otherwise, term " installation ", " connected ", " connection ", " fixation " etc.
Term shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integral；It can be mechanical connect
It connects, is also possible to be electrically connected；It can be directly connected, can also can be in two elements indirectly connected through an intermediary
The interaction relationship of the connection in portion or two elements.It for the ordinary skill in the art, can be according to specific feelings
Condition understands the concrete meaning of above-mentioned term in the present invention.
Refering to fig. 1-6, the utility model provides a kind of microscope example fixed frame, and structure is simple, easy to operate, this is aobvious
Micro mirror sample holder includes the fixing piece 2 of bracket 1 and installation on the bracket, and the bracket 1 includes support board 103, institute
It states fixing piece 2 and the support board 103 is by magnetic attraction mode secure fit.
Such as Fig. 1-2, the bracket further includes transverse slat 101 and riser 102,102 both ends of riser respectively with the transverse slat
101 and support board 103 connect, form integrally connected, constitute " Z " type structure, the transverse slat 101 connects with three-dimensional mobile platform
It connects, the support board 103 is placed in imaging storehouse.
Such as Fig. 1 and Fig. 3-6, several first grooves 3 are offered on the support board 103, are fixed in first groove 3
First magnet 301, number, shape and the position distribution of first magnet 301 are identical as the first groove 3, and the sample is solid
Determining part 2 is iron nail or magnetic nail or iron plate.Fixing piece 2 is realized using the magnetic attraction mode between iron and magnetic or magnetic and magnetic
Secure fit between support board 103.
Such as Fig. 2, the support board 103 is iron plate, and the fixing piece 2 is magnetic nail.Using the magnetic attraction between magnetic and iron
Mode realizes the secure fit between sample fixing piece 2 and support board 103.
Such as Fig. 7, the lower plane of the transverse slat 101 offers several second grooves 4, and is fixed in second groove 4
Two magnet 401, the transverse slat 101 and three-dimensional mobile platform are connected by magnetic attraction mode.
Wherein, in embodiment 1 and embodiment 3-6, support board 103 is using iron or stainless steel or plastic or other material system
At opening up several first grooves 3 on support board 103, the first groove 3 is arbitrary shape, position and quantity, in first groove 3
It is fixed with corresponding first magnet, the fixing means of magnet herein uses routine techniques in the art, it is only necessary to so that magnet
It is stably fixed at 3 in the first groove；Sample is clamped or is fixed on first using iron nail or magnetic nail or iron plate by fixing piece 2
On fixing piece 2, then the fixing piece for clamping or being fixed with sample is moved on on support board 103, so that fixing piece 2 and support board
The first magnet 301 in 103 first grooves 3 guarantees sample in microscope example by magnetic attraction mode secure fit
Fixed stability on fixed frame.
In embodiment 2, support board 103 is iron plate, and fixing piece 2 is followed closely using magnetic, after magnetic follows closely puncture sample, will be punctured with magnetic nail
Sample on support board 103, by magnetic nail and iron plate magnetic attraction secure fit, to guarantee that sample is firmly secured at
On support board.
Such as Fig. 7,101 lower plane of transverse slat offers several second grooves 4, is fixed with second in second groove 4
Magnet 401, the transverse slat 101 are connect with the three-dimensional mobile platform by magnetic attraction mode.The fixation side of magnet herein
Method uses routine techniques in the art, it is only necessary to so that the second magnet is stably fixed at 3 in the second groove, the second groove
Shape, position and quantity be it is any, the second magnet 401 is matched.
In addition, the sample holder structure in the utility model is not limited to " Z " type structure, " L " type etc. can also be used and be applicable in
In the supporting structure that other microscope examples are fixed.
The microscope example fixed frame stability fixed to sample is improved in the way of magnetic attraction, and do not influence sample at
Image quality amount, specific fixing means include: insertion fixation, adhesion fixation and grip method；
Examples 1 and 2, the insertion fixation is: selecting iron nail or magnetic to follow closely for the fixing piece 2, the thorn of fixing piece 2
It is in vertical after wearing sample, the fixing piece 2 is fixed with first magnet 301 by magnetic attraction, i.e. embodiment 1；Or
Selecting magnetic nail is the fixing piece 2, is in vertical after the fixing piece 2 pierces through sample, the fixing piece 2 and the support board
103 are directly fixed by magnetic attraction, and support board 103 is made at this time of ferrous material, i.e. embodiment 2.
Specifically, the cuspidated tiny iron nail of the tool such as cone or diamond shape or needle are inserted perpendicularly into sample to be imaged,
Insertion position can be any, avoids crucial imaging region in principle, when such as research object being cerebral hippocampus area, is embedded in iron nail or magnetic nail
When need to avoid hippocampus.Sample after puncture is in vertical, is carefully moved on support board, iron nail or magnetic nail on sample
Magnetic force is passed through by the support board of magnetic nail and irony in magnetic attraction secure fit or sample with the magnet on support board
It is fixed cooperation, and then guarantees that sample is stably fixed on microscope example fixed frame, guarantees the quality of imaging.
Preferably to fix sample, more insertion iron nails or magnetic can follow closely according to the actual situation, it is ensured that the fixed stability of sample.
It has tested simultaneously, under ferromagnetism effect, generally can be completely fixed sample using two thinner iron nails.
Insertion fixation is suitable for CUBIC, softer sample after the water solubility transparence method processing such as CLARITY.
Embodiment 3, the adhesion fixation is: selecting iron plate for the fixing piece 2, by its surface coating glue, sample
It directly sticks on the fixing piece 2, the fixing piece 2 is fixed with first magnet 301 by magnetic attraction；
Specifically, glue is coated on iron plate first, glue generally uses rapid-curing cutback glue, such as 502 glue, and iron plate is any
Shape need to ensure that the contact surface of iron plate and sample can support sample, then adhere to sample to be imaged on iron plate 2, to sample
It after adhesion is secured, is moved on support board, the first magnet 301 in iron plate lower plane and support board 103 passes through magnetic attraction
Secure fit.
Adhesion fixation is suitable for the sample of all transparence methods processing, especially BABB, uDISCO/iDISCO,
Harder sample after the processing of the organic solvents transparence method such as PEGASOS.
Embodiment 4-6, the method that grips is: selecting iron plate for the fixing piece 2, is made into and sample topography
The clamping shape of cooperation, clamps sample, and the fixing piece 2 is fixed with the first magnet 301 by magnetic attraction.
Specifically, iron plate is fabricated to and the matched clamping of sample topography to be imaged by the modes such as being bent, shearing first
Shape, in embodiment 4, two L-shaped structures are made in iron plate, clamp sample from the both sides of sample, in embodiment 5, iron plate production
The clamp structure of support is equipped at bottom plate quadrangle, from the bottom of sample and quadrangle support sample, embodiment 6, iron plate is made instead
To U-typed structure, sample is clamped above sample；Wherein, the specific structure of fixing piece 2 depends on specific to be imaged
The shape of sample secondly, the fixing piece made from iron plate with sample topography cooperation clamps sample, then will be clamped with consolidating for sample
Determine part and move to support board, the fixing piece of irony and the first magnet 301 of loading class pass through magnetic attraction secure fit.
Method is gripped suitable for BABB, after the processing of the organic solvents transparence method such as uDISCO/iDISCO, PEGASOS
Harder sample, or the sample with specific shape, such as the femur of mouse.
Finally, it should be noted that the above various embodiments is only to illustrate the technical solution of the utility model, rather than it is limited
System；Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should
Understand: it is still possible to modify the technical solutions described in the foregoing embodiments, or to some or all of
Technical characteristic is equivalently replaced；And these are modified or replaceed, it does not separate the essence of the corresponding technical solution, and this is practical new
The range of each embodiment technical solution of type.
Claims (5)
1. a kind of microscope example fixed frame, including bracket and rack-mount fixing piece, which is characterized in that the bracket
Including support board, the fixing piece and the support board are by magnetic attraction mode secure fit.
2. microscope example fixed frame according to claim 1, which is characterized in that the bracket further includes transverse slat and erects
Plate, the riser both ends are connect with the transverse slat and the support board respectively, and the transverse slat is connect with three-dimensional mobile platform, described
Support board is placed in imaging storehouse.
3. microscope example fixed frame according to claim 1 or 2, which is characterized in that if being offered on the support board
Dry first groove, is fixed with the first magnet in first groove, number, shape and the position distribution of first magnet with
First groove is identical, and the fixing piece is iron nail or magnetic nail or iron plate.
4. microscope example fixed frame according to claim 1 or 2, which is characterized in that the support board is iron plate, described
Fixing piece is magnetic nail.
5. microscope example fixed frame according to claim 2, which is characterized in that the transverse slat lower plane offers several
Second groove, the second magnet is fixed in second groove, and the transverse slat and the three-dimensional mobile platform pass through magnetic attraction
Mode connects.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822219995.7U CN209167676U (en) | 2018-12-27 | 2018-12-27 | A kind of microscope example fixed frame |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822219995.7U CN209167676U (en) | 2018-12-27 | 2018-12-27 | A kind of microscope example fixed frame |
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| Publication Number | Publication Date |
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| CN209167676U true CN209167676U (en) | 2019-07-26 |
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| CN201822219995.7U Active CN209167676U (en) | 2018-12-27 | 2018-12-27 | A kind of microscope example fixed frame |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110568605A (en) * | 2019-08-30 | 2019-12-13 | 北京临近空间飞行器系统工程研究所 | Positioning assembly and microscope |
| CN110673323A (en) * | 2019-10-16 | 2020-01-10 | 西湖大学 | Microscope assembly |
| CN110687670A (en) * | 2019-10-16 | 2020-01-14 | 西湖大学 | Flat light sheet microscope and using method thereof |
-
2018
- 2018-12-27 CN CN201822219995.7U patent/CN209167676U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110568605A (en) * | 2019-08-30 | 2019-12-13 | 北京临近空间飞行器系统工程研究所 | Positioning assembly and microscope |
| CN110673323A (en) * | 2019-10-16 | 2020-01-10 | 西湖大学 | Microscope assembly |
| CN110687670A (en) * | 2019-10-16 | 2020-01-14 | 西湖大学 | Flat light sheet microscope and using method thereof |
| CN110673323B (en) * | 2019-10-16 | 2021-11-23 | 锘海生物科学仪器(上海)有限公司 | Microscope assembly |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address |
Address after: Room 102 and 202, building 1, No. 650, Shunqing Road, Jiuting Town, Songjiang District, Shanghai 201600 Patentee after: Fuhai bioscience instrument (Shanghai) Co.,Ltd. Address before: Room 1203, No. 650, Xinzhuan Road, Songjiang District, Shanghai 201600 Patentee before: NUOHAI LIFE SCIENCE (SHANGHAI) Co.,Ltd. |
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| CP03 | Change of name, title or address | ||
| CP02 | Change in the address of a patent holder |
Address after: Room 201 and 202, No. 17, Lane 158, Yanzhan Road, Jiuting Town, Songjiang District, Shanghai, 201600 Patentee after: Fuhai bioscience instrument (Shanghai) Co.,Ltd. Address before: Room 102 and 202, building 1, No. 650, Shunqing Road, Jiuting Town, Songjiang District, Shanghai 201600 Patentee before: Fuhai bioscience instrument (Shanghai) Co.,Ltd. |
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| CP02 | Change in the address of a patent holder |