CN110673323B

CN110673323B - Microscope assembly

Info

Publication number: CN110673323B
Application number: CN201910982247.0A
Authority: CN
Inventors: 高亮
Original assignee: Fuhai Bioscience Instrument Shanghai Co ltd
Current assignee: NUOHAI LIFE SCIENCE (SHANGHAI) Co.,Ltd.
Priority date: 2019-10-16
Filing date: 2019-10-16
Publication date: 2021-11-23
Anticipated expiration: 2039-10-16
Also published as: CN110673323A

Abstract

Embodiments of the present disclosure provide a microscope assembly including an imaging chamber and a base. The imaging chamber is used for containing an imaging buffer solution, and a first positioning piece comprising a plurality of first absorption points is arranged at the bottom of the imaging chamber. The base is configured to bear the weight of the formation of image room and provide the waste liquid pond, be equipped with the second setting element that can actuation first setting element on the base, the second setting element has a plurality of second actuation points with a plurality of first actuation point one-to-ones, when being close to the base as the formation of image room, first actuation point can rather than corresponding second actuation point automatic actuation, realize that operating personnel can remove the formation of image room fast through simple operation, and can install the formation of image room when the base in needs, make the formation of image room install to the assigned position through first setting element and the automatic actuation of second setting element, need not the mounted position of the manual alignment formation of image room of operating personnel, when guaranteeing the accuracy of formation of image room recurrence location, can also avoid manual operation's error effectively.

Description

Microscope assembly

Technical Field

The present disclosure relates to the field of microscopic optical imaging technology, and more particularly, to a microscope assembly.

Background

Microscopes are widely used in life sciences and medicine. The microscope is an optical instrument formed by one lens or a combination of a plurality of lenses, and is widely applied to the fields of medical health, biological detection, metallographic detection, integrated circuit detection and the like. The specimen is typically placed on a stage and the specimen is viewed by movement of the specimen holder, e.g., in the X-direction and Y-direction.

However, the imaging chamber of the microscope in the prior art mostly adopts a non-detachable installation mode, which is not beneficial to replacing the imaging buffer solution in the imaging chamber, and a small number of the imaging chambers adopt a detachable installation mode, so that the imaging chamber is installed on the base in a mode that an operator needs to manually reset after detaching the imaging chamber, and the imaging buffer solution in the imaging chamber needs to be frequently replaced, so that the operation difficulty of the operator is increased, the manual reset accuracy is poor, and the operator cannot accurately position the imaging chamber.

Disclosure of Invention

In view of the above-mentioned problems in the prior art, the present disclosure provides a microscope assembly that allows for rapid removal and repositioning of an imaging chamber of the microscope assembly to facilitate rapid replacement of a biological sample to be imaged and an imaging buffer within the imaging chamber.

Embodiments of the present disclosure provide a microscope assembly comprising:

the imaging chamber is used for containing an imaging buffer solution, and a first positioning piece comprising a plurality of first absorption points is arranged at the bottom of the imaging chamber;

the base, its configuration is for bearing the weight of the formation of image room just provides the waste liquid pond, be equipped with on the base and inhale the second setting element of first setting element, the second setting element have with a plurality of second actuation points of first actuation point one-to-one, in order to work as the formation of image room is close to during the base, first actuation point can be rather than corresponding second actuation point automatic actuation.

In some embodiments, the microscope assembly further comprises a translation stage and a sample holder, one end of the sample holder is detachably connected to the translation stage, and the other end of the sample holder is provided with a sample carrier for extending into the imaging chamber, and the sample carrier is used for carrying a sample.

In some embodiments, two clamping members are oppositely arranged on the sample carrier, a clamping cavity for clamping the sample is formed between the two clamping members, and a liquid immersion groove opposite to the clamping cavity is formed on the sample carrier.

In some embodiments, at least one first magnetic member is disposed on the sample carrier.

In some embodiments, the at least one first magnetic member is configured to be attracted to a second magnetic member at the bottom of a custom piece configured to fit the geometry of a sample and to carry the sample.

In some embodiments, the at least one first magnetic element is configured to carry the sample and is pricked through the sample by a retaining needle to prick the sample.

In some embodiments, a groove is formed in the bottom of the imaging chamber, the first positioning member is disposed in the groove, and a protrusion portion for being embedded in the groove is disposed on the base, so that when the imaging chamber is disposed on the base, the upper surface of the second positioning member is attached to the lower surface of the first positioning member.

In some embodiments, at least one of the grips is slidably disposed on the sample carrier to adjust the size of the grip cavity.

In some embodiments, the clip is a sheet having some elasticity.

In some embodiments, the plurality of first engaging points comprises a first set of engaging members and a second set of engaging members, the first set of engaging members being in a triangular arrangement and wherein each engaging member is a partial surface of a steel ball, the second set of engaging members comprising at least two flat engaging members;

the plurality of second attraction points comprise a third group of attraction parts and a fourth group of attraction parts, the third group of attraction parts are constructed to be attracted by the first group of attraction parts, each attraction part is an arc-shaped concave part tightly attached to the partial surface of the steel ball, and the fourth group of attraction parts are constructed to be attracted by the second group of attraction parts.

In some embodiments, one end of the sample rack is attracted to the translation stage by at least two third magnetic members.

Compared with the prior art, the beneficial effects of the embodiment of the present disclosure are that: the invention realizes that when an operator can quickly remove the imaging chamber through simple operation and can install the imaging chamber on the base when the operator needs to install the imaging chamber on the base, the imaging chamber is arranged at the designated position by the automatic attraction of the first positioning piece and the second positioning piece without the need of manually aligning the installation position of the imaging chamber by an operator, can effectively avoid errors of manual operation while ensuring the reproduction positioning accuracy of the imaging room, and the first positioning piece and the second positioning piece can be automatically attracted, so that the operation and the use of personnel are convenient, in addition, the rapid removal and repositioning of the imaging chamber of the microscope assembly also enables the ready rapid exchange of the biological sample to be imaged and the imaging buffer within the imaging chamber.

Drawings

In the drawings, which are not necessarily drawn to scale, like reference numerals may describe similar components in different views. Like reference numerals having letter suffixes or different letter suffixes may represent different instances of similar components. The drawings illustrate various embodiments generally by way of example and not by way of limitation, and together with the description and claims serve to explain the disclosed embodiments. The same reference numbers will be used throughout the drawings to refer to the same or like parts, where appropriate. Such embodiments are illustrative, and are not intended to be exhaustive or exclusive embodiments of the present apparatus or method.

FIG. 1 is a schematic view of a partial structure of a microscope assembly according to an embodiment of the disclosure;

FIG. 2 is a partial exploded view of a microscope assembly according to an embodiment of the disclosure;

FIG. 3 is a schematic diagram of an imaging chamber of a microscope assembly according to an embodiment of the disclosure;

FIG. 4 is a schematic view of a first configuration of a sample holder of a microscope assembly according to an embodiment of the disclosure;

FIG. 5 is a second schematic view of a sample holder of a microscope assembly according to an embodiment of the disclosure;

FIG. 6 is a third schematic view of a sample holder of a microscope assembly according to an embodiment of the disclosure;

fig. 7 is a fourth structural schematic diagram of a sample holder of a microscope assembly according to an embodiment of the disclosure.

The members denoted by reference numerals in the drawings:

1-an imaging chamber; 11-a first positioning member; 12-a first absorption point; 13-a groove; 14-a first set of engaging members; 15-a second set of engaging members; 2-a base; 21-a second positioning element; 22-a second attraction point; 23-a boss; 24-a third set of engaging members; 25-a fourth set of engaging members; 3-a translation stage; 4-a sample rack; 41-a sample carrier; 42-a clamp; 43-immersion liquid groove; 5-a first magnetic member; 6-customizing a workpiece; 7-a limit needle; 8-a third magnetic element.

Detailed Description

For a better understanding of the technical aspects of the present disclosure, reference is made to the following detailed description taken in conjunction with the accompanying drawings. Embodiments of the present disclosure are described in further detail below with reference to the figures and the detailed description, but the present disclosure is not limited thereto.

The use of "first," "second," and similar terms in this disclosure is not intended to indicate any order, quantity, or importance, but rather are used to distinguish one element from another. The word "comprising" or "comprises", and the like, means that the element preceding the word covers the element listed after the word, and does not exclude the possibility that other elements are also covered. "upper", "lower", "left", "right", and the like are used merely to indicate relative positional relationships, and when the absolute position of the object being described is changed, the relative positional relationships may also be changed accordingly.

In the present disclosure, when a specific device is described as being located between a first device and a second device, there may or may not be intervening devices between the specific device and the first device or the second device. When a particular device is described as being coupled to other devices, that particular device may be directly coupled to the other devices without intervening devices or may be directly coupled to the other devices with intervening devices.

All terms (including technical or scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs unless specifically defined otherwise. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the relevant art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.

Techniques, methods, and apparatus known to those of ordinary skill in the relevant art may not be discussed in detail but are intended to be part of the specification where appropriate.

The disclosed embodiments provide a microscope assembly, as shown in fig. 1-3, comprising an imaging chamber 1 and a base 2. The imaging chamber 1 is used for containing imaging buffer solutions, different imaging buffer solutions can be contained in different imaging chambers 1, and can also be contained in the same imaging chamber 1, the disclosure is not specifically limited, but when the imaging buffer solution in the imaging chamber 1 is replaced by another imaging buffer solution, the imaging chamber 1 should be cleaned and dried comprehensively to ensure the accuracy of the detection result. The bottom of the imaging chamber 1 is provided with a first positioning member 11 comprising a plurality of first suction points 12. The base 2 is configured to bear the imaging chamber 1 and provide a waste liquid pool, the waste liquid pool is used for containing imaging buffer solution overflowing from the imaging chamber 1, a liquid leakage port can be formed in the side wall of the imaging chamber 1, redundant imaging buffer solution can be discharged from the liquid leakage port and flows to the waste liquid pool, a second positioning part 21 capable of attracting the first positioning part 11 is arranged on the base 2, the second positioning part 21 is provided with a plurality of second attraction points 22 corresponding to the first attraction points 12 one by one, when the imaging chamber 1 is close to the base 2, the first attraction points 12 can be automatically attracted with the corresponding second attraction points 22, the shape formed by sequentially connecting the first attraction points 12 is unlimited, the imaging chamber 1 can be stably installed on the base 2, and the first attraction points 12 and the second attraction points 22 can be formed by magnetic materials or other modes capable of generating a magnetic field.

This is disclosed through the first setting part 11 that contains a plurality of first attraction points 12 in the bottom setting of formation of image room 1, and set up the second setting part 21 that can actuation first setting part 11 on base 2, realize that can remove formation of image room 1 through simple operation fast at operating personnel, and can install formation of image room 1 in base 2 when needs, make formation of image room 1 install to the assigned position through first setting part 11 and the automatic actuation of second setting part 21, need not the manually aligned installation position of formation of image room 1 of operating personnel, when guaranteeing the precision that formation of image room 1 reappears the location, can also avoid manual operation's error effectively, and actuation can take place voluntarily for first setting part 11 and second setting part 21, the personnel of being convenient for operate and use.

In some embodiments, the microscope assembly further includes a translation stage 3 and a sample rack 4 (as shown in fig. 4), the translation stage 3 drives a sample on the sample rack 4 to move in the imaging chamber 1, so as to meet the requirement of an operator for observing the sample comprehensively, one end of the sample rack 4 is detachably connected to the translation stage 3, so that when the operator needs to replace the sample on the sample rack 4, the sample rack 4 can be detached from the translation stage 3 for operation, the other end of the sample rack 4 is provided with a sample carrier 41 for extending into the imaging chamber 1, the sample carrier 41 is used for carrying the sample, and the sample carrier 41 should reduce the occupied space as much as possible to increase the movable distance of the sample in the imaging chamber 1 on the premise of ensuring the sample is placed stably. Above-mentioned detachable sample frame 4 is convenient for operating personnel to place the sample on sample frame 4, and sample frame 4 can be connected in translation platform 3 through the mode of magnetic part or joint, can set up on sample frame 4 in addition and subtract heavy groove, when reducing the dead weight of sample frame 4, can also weaken the influence of inertia to sample frame 4 motion.

In some embodiments, two opposite clamping members 42 (as shown in fig. 4 and 5) are disposed on the sample carrier 41, a clamping cavity for clamping the sample is formed between the two clamping members 42, an operator can place the sample in the clamping cavity, the two clamping members 42 respectively abut against and fix the clamped sample, the material of the clamping members 42 is not limited, and the sample can be clamped after repeatedly loading and unloading the sample, the sample carrier 41 is provided with an immersion liquid groove 43 disposed opposite to the clamping cavity, the imaging buffer in the imaging chamber 1 can be immersed from the immersion liquid groove 43, so as to completely immerse the sample, thereby effectively preventing one side of the clamped sample close to the sample carrier 41 from being immersed by the imaging buffer, the shape of the immersion liquid groove 43 is not limited, and the flow of the imaging buffer can be achieved, which is not specifically limited herein. By the clamping action of the clamp 42, the operator is able to achieve rapid loading and unloading of the sample.

In some embodiments, at least one first magnetic member 5 is disposed on the sample carrier 41, and the sample is fixed on the sample carrier 41 by the magnetic attraction of the first magnetic member 5, so as to effectively avoid damaging the shape and structure of the sample itself, the number of the first magnetic members 5 is not limited, and the sample can be stably fixed, preferably, two first magnetic members 5 are disposed, and the space between the two first magnetic members 5 can accommodate the sample.

In some embodiments, as shown in fig. 6, at least one first magnetic member 5 is configured as a second magnetic member (not shown in the drawings) that is attached to the bottom of the customizing member 6, the customizing member 6 is configured to fit the geometric shape of the sample and is used for bearing the sample, in order to adapt to the specificity of the shape and structure of different samples, by manufacturing the customizing member 6 that is adapted to the shape of the specific sample to accommodate the sample, and the bottom of the customizing member 6 is provided with the second magnetic member that can be attached to the first magnetic member 5, while the customizing member 6 can be accurately positioned on the sample carrier 41, the adaptability of the product can be increased, the fixing requirement of samples with different shapes can be met without damaging the shape and structure of the sample, and the customizing member 6 can be formed by laser cutting or injection molding, which is not specifically limited in this disclosure.

In some embodiments, at least one first magnetic member 5 is configured to carry a sample, and is inserted through the sample by a retaining pin 7 to secure the sample (as shown in fig. 7), the retaining pin 7 can interact with the first magnetic member 5 to stably secure the sample on the first magnetic member 5, and prevent the sample from being detached from the sample carrier 41 after being immersed in an imaging buffer solution, the above solution is suitable for securing a relatively flat sample, the number of the retaining pins 7 is not limited, and the sample can be stably secured without destroying the structure of the sample.

In some embodiments, a groove 13 (as shown in fig. 2) is formed at the bottom of the imaging chamber 1, the first positioning member 11 is disposed in the groove 13, and a protrusion 23 for being embedded in the groove 13 is disposed on the base 2, so that when the imaging chamber 1 is disposed on the base 2, the upper surface of the second positioning member 21 is attached to the lower surface of the first positioning member 11, and the protrusion 23 is protruded in the waste liquid pool, so as to prevent the waste liquid in the waste liquid pool from soaking in the waste liquid pool for a long time to contaminate the second positioning member 21, thereby causing the second positioning member 21 to fail. The grooves 13 may extend through two opposite sides of the imaging chamber 1, or may be closed grooves 13, and preferably, the grooves 13 extend through two opposite sides of the imaging chamber 1, which is convenient for processing and can reduce material cost. In addition, the matching of the groove 13 and the bulge 23 can beautify the appearance of the product.

In some embodiments, at least one grip 42 is slidably disposed on the sample carrier 41 to adjust the size of the grip cavity for increased product flexibility, such that the grip 42 can grip samples of different sizes. The slidable connection mode is not particularly limited in the disclosure, for example, the slidable connection mode is connected by adopting a sliding rail sliding block or a sliding shaft sleeve, so that the cost is saved, and meanwhile, the operation of an operator is facilitated.

In some embodiments, the grip 42 is a sheet with a certain elasticity to clamp the sample by elastic deformation of the grip 42, and the material of the sheet is preferably a material that is non-contaminating and has good resilience, such as spring steel, stainless steel, and the like.

In some embodiments, with reference to fig. 2 and 3, the plurality of first engaging points 12 includes a first engaging member 14 and a second engaging member 15, the first engaging member 14 is disposed in a triangular shape and each engaging member is a partial surface of a steel ball, the triangular three-point positioning can position the imaging chamber 1 at a specific position more precisely, and the steel ball engages with the second engaging point 22 in a point-to-point manner, only when the top surface of the steel ball engages with the second engaging point 22, the imaging chamber 1 can be stably disposed on the base 2, and further a fixed position of the imaging chamber 1 mounted on the base 2 is positioned, the second engaging member 15 includes at least two flat engaging members, since the contact area between the steel ball and the second engaging point 22 is small, which easily causes the mounting relationship between the imaging chamber 1 and the base 2 to be unstable, the second engaging member 15 is provided, the first attraction point 12 and the second attraction point 22 are attracted in a face-to-face mode, so that the contact area is increased, the attraction force generated between the imaging chamber 1 and the base 2 is increased, and the installation stability of the imaging chamber 1 is improved; the plurality of second suction points 22 includes a third suction member 24 and a fourth suction member 25, the third suction member 24 is configured to be sucked with the first suction member 14, and each of the suction members is an arc-shaped recess closely attached to a part of a surface of the steel ball so that the steel ball can be partially inserted into the arc-shaped recess, thereby further increasing the installation stability of the imaging chamber 1, the fourth suction member 25 is configured to be sucked with the second suction member 15, and the fourth suction member 25 preferably adopts the same structure as the flat structure of the second suction member 15 to increase the suction force between the fourth suction member 25 and the second suction member 15.

In some embodiments, with reference to fig. 5, one end of the sample rack 4 is attracted to the translation stage 3 through at least two third magnetic members 8, and the sample rack 4 is connected to the translation stage 3 through two-point positioning, so that the mounting stability of the sample rack 4 is improved, and the third magnetic members 8 are convenient to mount and low in cost.

Moreover, although exemplary embodiments have been described herein, the scope thereof includes any and all embodiments based on the disclosure with equivalent elements, modifications, omissions, combinations (e.g., of various embodiments across), adaptations or alterations. The elements of the claims are to be interpreted broadly based on the language employed in the claims and not limited to examples described in the present specification or during the prosecution of the application, which examples are to be construed as non-exclusive. It is intended, therefore, that the specification and examples be considered as exemplary only, with a true scope and spirit being indicated by the following claims and their full scope of equivalents.

The above description is intended to be illustrative and not restrictive. For example, the above-described examples (or one or more versions thereof) may be used in combination with each other. For example, other embodiments may be used by those of ordinary skill in the art upon reading the above description. In addition, in the foregoing detailed description, various features may be grouped together to streamline the disclosure. This should not be interpreted as an intention that a disclosed feature not claimed is essential to any claim. Rather, the subject matter of the present disclosure may lie in less than all features of a particular disclosed embodiment. Thus, the following claims are hereby incorporated into the detailed description as examples or embodiments, with each claim standing on its own as a separate embodiment, and it is contemplated that these embodiments may be combined with each other in various combinations or permutations. The scope of the disclosure should be determined with reference to the appended claims, along with the full scope of equivalents to which such claims are entitled.

The above embodiments are merely exemplary embodiments of the present disclosure, which is not intended to limit the present disclosure, and the scope of the present disclosure is defined by the claims. Various modifications and equivalents of the disclosure may occur to those skilled in the art within the spirit and scope of the disclosure, and such modifications and equivalents are considered to be within the scope of the disclosure.

Claims

1. A microscope assembly, comprising:

the base is configured to bear the imaging chamber and provide a waste liquid pool, a second positioning part capable of attracting the first positioning part is arranged on the base, the second positioning part is provided with a plurality of second attraction points in one-to-one correspondence with the first attraction points, and when the imaging chamber is close to the base, the first attraction points can be automatically attracted with the corresponding second attraction points;

the plurality of first suction points comprise a first group of suction parts and a second group of suction parts, the first group of suction parts are arranged in a triangular shape, each suction part is part of the surface of a steel ball, and the second group of suction parts comprise at least two flat suction parts;

a plurality of the second actuation point includes third group's suction member and fourth group's suction member, the third group suction member be constructed as with the first group suction member actuation, and wherein each suction member be with the arc concave part of the part surface of steel ball closely laminating, the fourth group suction member be constructed as with the second group suction member actuation.

2. The microscope assembly of claim 1, further comprising a translation stage and a sample holder removably connected at one end to the translation stage and provided at its other end with a sample carrier for extending into the imaging chamber, the sample carrier for carrying a sample.

3. The microscope assembly of claim 2, wherein the sample carrier has two opposing clamping members, a clamping chamber is formed between the two clamping members for clamping the sample, and the sample carrier has a liquid immersion groove disposed opposite to the clamping chamber.

4. The microscope assembly of claim 2, wherein the sample carrier has at least one first magnetic member disposed thereon.

5. The microscope assembly of claim 4, wherein the at least one first magnetic member is configured as a second magnetic member that snaps onto a bottom portion of a custom piece configured to fit a sample geometry and to carry the sample.

6. The microscope assembly of claim 4, wherein the at least one first magnetic member is configured to carry the sample and is penetrated by a retaining pin through the sample to secure the sample.

7. The microscope assembly of claim 1, wherein the imaging chamber has a recess in a bottom thereof, the first positioning member is disposed in the recess, and the base has a protrusion for nesting in the recess, such that an upper surface of the second positioning member is engaged with a lower surface of the first positioning member when the imaging chamber is disposed on the base.

8. A microscope assembly according to claim 3, wherein at least one of the grips is slidably arranged on the sample carrier to adjust the size of the grip cavity.

9. A microscope assembly according to claim 3, wherein the clip is a somewhat resilient tab.

10. The microscope assembly of claim 2, wherein one end of the sample holder is attracted to the translation stage by at least two third magnetic members.