CN209093397U - A kind of fluid interconnection adapter and microfluidic system for micro-fluidic chip - Google Patents
A kind of fluid interconnection adapter and microfluidic system for micro-fluidic chip Download PDFInfo
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- CN209093397U CN209093397U CN201821617278.3U CN201821617278U CN209093397U CN 209093397 U CN209093397 U CN 209093397U CN 201821617278 U CN201821617278 U CN 201821617278U CN 209093397 U CN209093397 U CN 209093397U
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Abstract
The embodiments of the present invention disclose a kind of fluid interconnection adapter for micro-fluidic chip, including microfluidic device and fluid separation systems with multiple swimming lanes, the microfluidic device includes top plate, middle plate, bottom plate and the micro-fluidic chip being arranged between top plate and middle plate, the top plate is connect with fluid separation systems, the micro-fluidic chip includes bottom plate and the cover board with bottom plate sealing cooperation, and middle layer is equipped between bottom plate and cover board, which is equipped with one or more main channel.Fluid interconnection adapter provided by the utility model for micro-fluidic chip has the advantages that structure is simple, separative efficiency is high, and flow direction is allowed to invert, and can allow for reagent mixing and/or fluid line cleaning, brings great convenience for user's use.
Description
Technical field
The embodiments of the present invention are related to microfluidic chip analysis technical field, and in particular to one kind is used for micro-fluidic core
The fluid interconnection adapter and microfluidic system of piece.
Background technique
ELISA (ELISA) as conventional analytical technology be widely used in clinical diagnosis, food safety detection,
The fields such as environment measuring.It is generally necessary to use microfluidic device during ELISA, but existing microfluid
Device low separation efficiency, structure is complicated makes troubles to people's use.
Utility model content
The purpose of this utility model is to provide a kind of fluid interconnection adapters and miniflow system for micro-fluidic chip
System, to solve the problems, such as that existing microfluidic device low separation efficiency, structure is complicated makes troubles to people's use.
To achieve the above object, the technical solution that the embodiments of the present invention provide are as follows: one kind is used for micro-fluidic chip
Fluid interconnection adapter, including microfluidic device and fluid separation systems with multiple swimming lanes, the microfluidic device
Including top plate, middle plate, bottom plate and the micro-fluidic chip being arranged between top plate and middle plate, the upper layer
Plate is connect with fluid separation systems, the micro-fluidic chip include bottom plate and with bottom plate sealing cooperation cover board, and bottom plate with
Middle layer is equipped between cover board, which is equipped with one or more main channel.
Among some embodiments, the both ends of the bottom plate are symmetrically arranged with two fixture blocks, and the cover board is equipped with and bottom
The card slot that fixture block on plate is used cooperatively, and the middle layer is equipped with the through-hole worn for the fixture block on bottom plate.
Among some embodiments, the middle layer is equipped with two main channels.
Among some more specific embodiments, one end of two main channels is arranged in parallel, and the other end collects
At a channel.
Among some embodiments, the both ends of two main channels are arranged in parallel respectively, and in two main channels
Portion intersects connection.
Among some embodiments, the both ends of two main channels are arranged in parallel respectively, the middle part of two main channels
Intersect connection, and two main channels form inwardly close extension far from one end of infall, the end of the extension is mutual
In parallel.
Among some embodiments, the bottom plate and cover board of the micro-fluidic chip are equipped with elastomer connector.
In addition, the utility model additionally provides a kind of microfluidic system, including any one it is above-mentioned be used for micro-fluidic chip
Fluid interconnection adapter.
Among some embodiments, the microfluidic system includes kit, above-mentioned for micro-fluidic chip
Adapter, output selector valve, micro-fluid pump and waste reservoir are fluidly interconnected,
When the reagent in the microfluidic system needs to flow through external micro-fluidic chip, the kit be adapted to
The input terminal of the input terminal connection of micro-fluidic chip in device, the output end of the micro-fluidic chip and external micro-fluidic chip connects
It connects, the output end of the external micro-fluidic chip and the input terminal of micro-fluid pump connect, the output end of the micro-fluid pump and institute
The input terminal connection of micro-fluidic chip in adapter is stated, and the output end of the micro-fluidic chip is also connected with micro-fluid pump；
When the reagent in the microfluidic system needs not flow through external micro-fluidic chip, the kit and suitable
The input terminal of micro-fluidic chip connects in orchestration, the input of the output end of micro-fluidic chip and output selector valve in the adapter
End connection, it is described output selector valve output end and micro-fluid pump input terminal connect, the output end of the micro-fluid pump respectively with
Waste reservoir is connected with the input terminal of micro-fluidic chip in adapter.
Compared with prior art, the embodiments of the present invention have the advantages that the embodiments of the present invention provide
A kind of fluid interconnection adapter and microfluidic system for micro-fluidic chip, the adapter structure is simple, separative efficiency is high,
And flow direction is allowed to invert, it can allow for reagent mixing and/or fluid line cleaning, bring great convenience for user's use.
Detailed description of the invention
Fig. 1 is in one exemplary embodiments of the utility model for whole after the fluid interconnection adapter assembling of micro-fluidic chip
Stereogram；
Fig. 2 is the exploded view in one exemplary embodiments of the utility model for the fluid interconnection adapter of micro-fluidic chip；
Fig. 3 is that the fluid interconnection adapter removal in one exemplary embodiments of the utility model for micro-fluidic chip is micro-fluidic
Structural schematic diagram after chip；
Fig. 4 is the structural schematic diagram of micro-fluidic chip in one exemplary embodiments of the utility model；
Fig. 5 a is one of the structural schematic diagram of middle layer of micro-fluidic chip in Fig. 4；
Fig. 5 b is the second structural representation of the middle layer of micro-fluidic chip in Fig. 4；
Fig. 5 c is the third structural representation of the middle layer of micro-fluidic chip in Fig. 4；
Fig. 6 is the structural schematic diagram of microfluidic system in one exemplary embodiments of the utility model；
Description of symbols: 1-microfluidic device；101-top plates；102-middle plates；103-bottom plates；2-streams
Body separation system；3-micro-fluidic chips；301-bottom plates；302-cover boards；303-middle layers；304-fixture blocks；305-cards
Slot；306-through-holes；4-main channels；5-adapters；6-kits；7-output selector valves；8-micro-fluid pumps；9-wastes
Pond；10-elastomer connectors.
Specific embodiment
The following examples illustrate the utility model, but is not intended to limit the scope of the present invention.
The utility model is described in detail in the following with reference to the drawings and specific embodiments, as shown in Fig. 1 and Fig. 2, this
The embodiment of utility model discloses a kind of fluid interconnection adapter for micro-fluidic chip, including with the micro- of multiple swimming lanes
Fluid means 1 and fluid separation systems 2, the microfluidic device 1 include top plate 101, middle plate 102, bottom plate 103,
And the micro-fluidic chip 3 between top plate 101 and middle plate 102, the top plate 101 and fluid separation systems are set
2 connections, as shown in figure 4, the micro-fluidic chip 3 includes bottom plate 301 and seals the cover board 302 of cooperation with bottom plate 301, and bottom
Middle layer 303 is equipped between plate 301 and cover board 302, which is equipped with one or more main channel.
Further, the both ends of the bottom plate 301 are symmetrically arranged with two fixture blocks 304, and the cover board 302 is equipped with and bottom
The card slot 305 that fixture block 304 on plate 301 is used cooperatively, and the middle layer 303 is equipped with for the fixture block on bottom plate 301
304 through-holes 306 worn.
Further, as shown in Figure 5 a, the middle layer 303 is equipped with two main channels 4.Two main channels 4
One end be arranged in parallel, the other end accumulates a channel；
Alternatively, as shown in Figure 5 b, the both ends of two main channels 4 are arranged in parallel respectively, and the middle part of two main channels 4
Intersect connection；
Alternatively, as shown in Figure 5 c, the both ends of two main channels 4 are arranged in parallel respectively, the middle part of two main channels 4 is handed over
Fork connection, and two main channels 4 form inwardly close extension far from one end of infall, the end of the extension is mutually flat
Row.
Further, as shown in figure 3, the bottom plate 301 of the micro-fluidic chip 3 and cover board 302 are equipped with elastomer connector
10。
In addition, the utility model additionally provides a kind of microfluidic system, including any one it is above-mentioned be used for micro-fluidic chip
Fluid interconnection adapter 5.
Further, the microfluidic system includes kit 6, above-mentioned 5, output selector valve 7,8 and of micro-fluid pump
Waste reservoir 9,
When the reagent in the microfluidic system flows through external micro-fluidic chip, the kit 6 and adapter 5
The input terminal of middle micro-fluidic chip 3 connects, and the input terminal of the output end of the micro-fluidic chip 3 and external micro-fluidic chip connects
Connect, the output end of the external micro-fluidic chip is connect with the input terminal of micro-fluid pump 8, the output end of the micro-fluid pump 8 and
The input terminal connection of micro-fluidic chip 3 in the adapter 5, and the output end of the micro-fluidic chip 3 is also connected with microfluid
Pump 8；
When the reagent in the microfluidic system needs not flow through external micro-fluidic chip, the kit 6 with
The input terminal of micro-fluidic chip 3 connects in adapter 5, the output end of micro-fluidic chip 3 and output selector valve in the adapter 5
7 input terminal connection, it is described output selector valve 7 output end connect with the input terminal of micro-fluid pump 8, the micro-fluid pump 8 it is defeated
Outlet is connect with the input terminal of micro-fluidic chip 3 in waste reservoir 9 and adapter 5 respectively.
The technical solution of the utility model is further described below in conjunction with an exemplary embodiments.
A kind of microfluidic system includes kit 6, has reagent container several in box, sip device pipe and reagent selector valve discrimination
Pipe.Selected reagent is entered in the micro-fluidic chip 3 of adapter 5 by the main delivery port of reagent.Under routinely manipulation, output selection
Valve 7 is in X position, thus selected reagent will flow through external micro-fluidic chip from the main delivery outlet of micro-fluidic chip 3, then by micro-
The effect of fluid pump 8, enters back into the micro-fluidic chip 3 of adapter 5 and exports after master and pump out.The above is essentially all logical
Hole, thus the micro-fluidic chip 3 of adapter 5 is without interfering reagent path.After micro-fluid pump 8 reaches its maximum capacity, reagent
Waste tank 9 will be pumped to.Contain a triple valve in pump, to ensure that liquid stream flows in the manner described above.When reagent needs directly
By the micro-fluidic chip 3 of adapter 5 rather than when external micro-fluidic chip flowing, output selector valve 7 will be switched to Y location.
Reagent will be entered micro-fluidic chip 3 by input hole, flow through it is micro-fluidic gush, pass through auxiliary after output be discharged and by output selection
Valve 7 reaches micro-fluid pump 8.At this point, the reagent being stored in micro-fluid pump 8 can not only be pumped to waste reservoir 9 but also can be injected again
In circulation.The above operation also can be used for system clean, when if you need to clean pipeline, to avoid external micro-fluidic chip by cleaning operation
Influence can start aforesaid operations.
As previously mentioned, the fluid figure of microfluid adapter as described herein can be used for but unlimited make in nucleic acid sequencing system
System or method.Optics or non-optical detection means can be used in these systems or method.When using optical detection means, adopt
With the sequencing approach in synthesis, nucleotide is incorporated into nucleic acid-templated, is arranged in clone's group of " cluster ", by using polymerase chain
Formula reaction method expands a primary template chain to generate cloned copies.Above two method can all be detected with fluorescence probe, such as
Using the terminator of dye marker, each base (A, C, T or G) is marked with different dyes.These dyestuffs issue different wave length
Fluorescence, with the use of filter plate thus to detect different nucleotide bases.Different bases containing fluorescent dye can simultaneously at
Picture or independent imaging.It needs to detect dyestuff using filter bank if being imaged simultaneously；Above-mentioned filtering is not necessarily to if independent imaging
System, without adding different dyes on nucleotide base.However the conveying of different nucleotide bases needs in different time points
It carries out, to meet detection demand.The advantage of the method is it is not necessary that dyestuff is added in nucleotide base.
Other sequencing means also can be used, such as when the dyestuff of different number is attached on nucleotide base.It illustrates,
In some embodiments, encoding mechanism can be used when need to only use two kinds of dyestuffs, two of them nucleotide base receives two kinds not
Same dyestuff, third nucleotide base receive the mixture of the two kinds of dyestuffs in front；4th nucleotide does not receive any dyestuff then.
By using the above method, 2 kinds of colors only need to be read on flow cell, reduce the complexity of detection system.Above-mentioned detection system
System, which can be integrated using two kinds of detection devices, detects this two kinds of dyestuffs, as CCD camera is used cooperatively and a kind of dye emission wavelength
Matched filter, or combined using a kind of detection device of optional filter, the filter wheel of filter such as with there are two,
To meet the needs of two kinds of dye emission wavelength.However this detection method is based on certain realization factor: first, third core
Thuja acid base should evenly receive two kinds of dyestuffs, to avoid another nucleotide base is mistaken for；Second, because of the 4th core
Thuja acid base does not receive any dyestuff, does not issue any light that can be detected, thus sequencing start before should execute calibration
Program, to detect cluster point position.Therefore, detection device controller/processor will be using the cluster o'clock that can not be detected as the 4th core
The processing of thuja acid base.Thus, controller/processor need to predict all cluster points position on flow cell, if upper in fixed position
It detects any dye emission light, then determines this for tetranucleotide base.
Said detecting system can be formed due to optical element of different nature, including but be not limited to following elements: different
Filter plate, tube lens, relay lens and the camera of wavelength.Camera can scan for CCD or CMOS and with sector scanning, line
Or TDI scanning function.Entire detection system includes 1-3 kind or the above camera, 1-3 kind or the above filter plate or both the above
The composition element of element.In experiment, flow cell be placed in can X and Y-direction movement example platform on, and camera, filter plate and thoroughly
Mirror remain stationary state.To ensure smoothly to capture image, the movement of platform keeps synchronous with the shooting of camera, then with specific
Image processing software handles captured image.The software can position the position of each nucleic acid cluster, by receiving by every
Fluorescence after a filter plate determines the nucleic acid base after synthesis.Later, the base after measurement and its accurate statistical value (Q
Value) it will be recorded in the form of Standard File Format.
Another and above-mentioned microfluid adapter detection method is parallel optical detection hand using the means for doing detection of nucleic acids
The excitation system of section.It includes 1,2,3 or more light source, such as laser or LED.Above-mentioned light source can provide suitable on different wave length
When illumination, with meet nucleic acid sequencing detection dyestuff excitation wavelength demand.Any component of above-mentioned optical element can be used.
Microfluid adapter as described herein uses in many cases system, method and embodiment although also needing to repair.
In addition to this microfluid adapter can be used in the instrument of future exploitation, system or method, but not limited to this.
Although above having made detailed description to the utility model with generality explanation and specific embodiment,
On the basis of the utility model, it can be made some modifications or improvements, this is aobvious and easy to those skilled in the art
See.Therefore, these modifications or improvements on the basis of without departing from the spirit of the present invention, belong to the utility model
Claimed range.
Claims (9)
1. a kind of fluid interconnection adapter for micro-fluidic chip, it is characterised in that: including the microfluid with multiple swimming lanes
Device and fluid separation systems, the microfluidic device include top plate, middle plate, bottom plate and are arranged in top plate
Micro-fluidic chip between middle plate, the top plate are connect with fluid separation systems, and the micro-fluidic chip includes
Bottom plate and the cover board cooperated with bottom plate sealing, and middle layer is equipped between bottom plate and cover board, which is equipped with one or more
Main channel.
2. the fluid interconnection adapter according to claim 1 for micro-fluidic chip, it is characterised in that: the bottom plate
Both ends are symmetrically arranged with two fixture blocks, and the cover board is equipped with the card slot being used cooperatively with the fixture block on bottom plate, and the centre
Layer is equipped with the through-hole worn for the fixture block on bottom plate.
3. the fluid interconnection adapter according to claim 1 or 2 for micro-fluidic chip, it is characterised in that: described
Middle layer is equipped with two main channels.
4. the fluid interconnection adapter according to claim 3 for micro-fluidic chip, it is characterised in that: two masters
The one end in channel is arranged in parallel, and the other end accumulates a channel.
5. the fluid interconnection adapter according to claim 3 for micro-fluidic chip, it is characterised in that: two masters
The both ends in channel are arranged in parallel respectively, and the middle part of two main channels intersects connection.
6. the fluid interconnection adapter according to claim 3 for micro-fluidic chip, it is characterised in that: two masters
The both ends in channel are arranged in parallel respectively, and the middle part of two main channels intersects connection, and the one end of two main channels far from infall
Inwardly close extension is formed, the end of the extension is parallel to each other.
7. the fluid interconnection adapter according to claim 1 for micro-fluidic chip, it is characterised in that: described micro-fluidic
The bottom plate and cover board of chip are equipped with elastomer connector.
8. a kind of microfluidic system, it is characterised in that: including being used for micro-fluidic chip described in any one of claim 1-7
Fluid interconnection adapter.
9. microfluidic system according to claim 8, it is characterised in that: including any in kit, claim 1-7
Described in for micro-fluidic chip fluid interconnection adapter, export selector valve, micro-fluid pump and waste reservoir,
When the reagent in the microfluidic system flows through external micro-fluidic chip, miniflow in the kit and adapter
The input terminal connection of chip is controlled, the output end of the micro-fluidic chip is connect with the input terminal of external micro-fluidic chip, described outer
The output end of portion's micro-fluidic chip and the input terminal of micro-fluid pump connect, in the output end of the micro-fluid pump and the adapter
The input terminal of micro-fluidic chip connects, and the output end of the micro-fluidic chip is also connected with micro-fluid pump；
When the reagent in the microfluidic system is without external micro-fluidic chip, miniflow in the kit and adapter
The input terminal connection of chip is controlled, the output end of micro-fluidic chip is connect with the input terminal of output selector valve in the adapter, institute
State output selector valve output end and micro-fluid pump input terminal connect, the output end of the micro-fluid pump respectively with waste reservoir and fit
The input terminal connection of micro-fluidic chip in orchestration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821617278.3U CN209093397U (en) | 2018-09-30 | 2018-09-30 | A kind of fluid interconnection adapter and microfluidic system for micro-fluidic chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821617278.3U CN209093397U (en) | 2018-09-30 | 2018-09-30 | A kind of fluid interconnection adapter and microfluidic system for micro-fluidic chip |
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| Publication Number | Publication Date |
|---|---|
| CN209093397U true CN209093397U (en) | 2019-07-12 |
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| CN201821617278.3U Active CN209093397U (en) | 2018-09-30 | 2018-09-30 | A kind of fluid interconnection adapter and microfluidic system for micro-fluidic chip |
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- 2018-09-30 CN CN201821617278.3U patent/CN209093397U/en active Active
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