CN209069692U - Hospital's urine excretion body monofilm enrichment system - Google Patents
Hospital's urine excretion body monofilm enrichment system Download PDFInfo
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- CN209069692U CN209069692U CN201821324206.XU CN201821324206U CN209069692U CN 209069692 U CN209069692 U CN 209069692U CN 201821324206 U CN201821324206 U CN 201821324206U CN 209069692 U CN209069692 U CN 209069692U
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- excretion body
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Abstract
The utility model is suitable for the field of medical instrument technology, provides a kind of hospital's urine excretion body monofilm enrichment system, comprising: the urine is carried out low-speed centrifugal processing and obtains supernatant by low speed centrifuge;Filtration membrane filtration system is enriched with the excretion body, with the low speed centrifuge split settings for filtering the impurity secreted except as noted other than body in the supernatant.Whereby, the hospital of the utility model is low in cost with urine excretion body monofilm enrichment system.
Description
Technical field
It is enriched with the utility model relates to the field of medical instrument technology more particularly to a kind of hospital with urine excretion body monofilm and is
System.
Background technique
Exosome, Chinese name excretion body are a kind of small film bubbles that can be secreted by most cells, have double-layer of lipoid
Membrane structure, diameter about 40~100nm.Although excretion body is initially just found in nineteen eighty-three, people think that always it is
A kind of waste of cell.However recent years, it has been found that the albumen containing cell-specific in this small film bubble, lipid and
Nucleic acid can pass to other cells as signaling molecule to change the function of other cells.People couple have been lighted in these discoveries
The interest of cell secretion film bubble.Nearest research discovery excretion body plays an important role on many physiological and pathologicals, such as immune
Middle antigen presentation, the growth of tumour and migration, the reparation of tissue damage etc..The excretion body of different cell secretions has unused group
At ingredient and function, the biomarker of medical diagnosis on disease can be used as.Excretion body has double-layer of lipoid membrane structure, can be well protected
Its coated substance, and specific cells or tissue can be targeted, therefore be a kind of fine targeting drug delivery system.Excretion body is as one
Novel research hotspot has become medical diagnosis on disease, treatment due to the convenience of its existing popularity and acquisition in vivo
Potential effective means, have bright prospect on accurate medical development.
Source of the urine as a kind of noninvasive detection sample, increasingly by everybody concern.Excretion body in urine
The molecular marked compound for carrying kidney structure and function damage, facilitates the diagnosis of kidney trouble;Urine excretion body protein group
Research facilitate the research of related disease pathophysiological mechanism, help to find potential new disease treatment target spot.
The main method of the excretion body of report separation both at home and abroad is supercentrifugation at present.
Supercentrifugation need ultracentrifuge only have prominent university, part scientific research institution, part developed regions three
First hospital possesses, and is limited so that this method uses;Secondly, this method single treatment sample size is limited.
In conclusion it is early for diabetic nephropathy with the enrichment of urine excretion body still to lack a kind of low-cost hospital at present
The device of phase diagnosis.
Utility model content
For above-mentioned defect, the purpose of this utility model is to provide a kind of hospital urine excretion body monofilms to be enriched with system
System, it is low in cost.
To achieve the goals above, the utility model provides a kind of hospital's urine excretion body monofilm enrichment system, packet
It includes:
The urine is carried out low-speed centrifugal processing and obtains supernatant by low speed centrifuge;
Filtration membrane filtration system is enriched with described outer for filtering the impurity secreted except as noted other than body in the supernatant
Body is secreted, with the low speed centrifuge split settings.
According to hospital urine excretion body monofilm enrichment system, the filtration membrane filtration system includes:
Sample cell, upper part are holding tank, and lower part is to be connected to the tubular conduit for accommodating trench bottom, the holding tank
Position corresponding to the tubular conduit offers opening, and the tubular conduit is connected to by the opening with the holding tank;
Filtration membrane, in a tubular form, the upper end are socketed on the outer wall of the tubular conduit, and lower end is folded with film folder, by described
Film folder is closed its lower end.
According to hospital urine excretion body monofilm enrichment system, the filtration membrane is that molecular cut off is
The filtration membrane of 1000KDa, for retaining the excretion body;
The length of the filtration membrane is 30 centimetres.
According to hospital urine excretion body monofilm enrichment system, the depth of the holding tank is greater than 20 centimetres.
According to hospital urine excretion body monofilm enrichment system, the filtration membrane filtration system further includes that waste liquid is received
Collect container, there is the waste collection chamber for holding waste liquid, when carrying out waste collection, the waste liquid collection vessel is socketed on the sample
The outside in product pond.
According to hospital urine excretion body monofilm enrichment system, the bottom of the waste liquid collection vessel is connected with bottom
Seat.
According to hospital urine excretion body monofilm enrichment system, the waste liquid collection vessel is in cylindrical;
The outer wall of the waste liquid collection vessel is made of transparent material.
According to hospital urine excretion body monofilm enrichment system, the hospital is enriched with urine excretion body monofilm is
System further includes that excretion body collects centrifuge tube, seperated with the filtration membrane filtration system to set for holding the excretion body of the enrichment
It sets, the capacity that the excretion body collects centrifuge tube is more than or equal to 5 milliliters.
According to hospital urine excretion body monofilm enrichment system, the hospital is enriched with urine excretion body monofilm is
System further includes graduated cylinder, and with the filtration membrane filtration system split settings, the graduated cylinder is made of transparent material, and the graduated cylinder
On be provided with graduation mark, the capacity of the graduated cylinder is 2000 milliliters.
According to hospital urine excretion body monofilm enrichment system, the hospital is enriched with urine excretion body monofilm is
System further includes sample bottle, and filtration membrane filtration system split settings, for holding described in the low speed centrifuge generates
Supernatant.
The hospital of the utility model urine excretion body monofilm enrichment system, is set as including low speed centrifuge and filtration
Film filtration system, low speed centrifuge are a kind of common centrifuges, do not need additional commercialization instrument and equipment, save cost,
Therefore, most of hospital can complete, and the urine is carried out low-speed centrifugal processing and obtains supernatant;Filtration membrane filtration system is used
External impurity is secreted except as noted in filtering in the supernatant, with the low speed centrifuge split settings.According to the excretion
The own physical property of body, is filtered by filtration membrane filtration system, and high degree ensure that the excretion body of enrichment
Purity and physicochemical property.Whereby, the hospital of the utility model is low in cost with urine excretion body monofilm enrichment system.
Detailed description of the invention
Fig. 1 is the modular structure signal of hospital provided by the embodiment of the utility model urine excretion body monofilm enrichment system
Figure;
Fig. 2 is that hospital provided by the embodiment of the utility model is shown with the sample pool structure of urine excretion body monofilm enrichment system
It is intended to;
Fig. 3 is the filtration membrane filtration system of hospital provided by the embodiment of the utility model urine excretion body monofilm enrichment system
The use state diagram of system;
Fig. 4 is waste liquid collection vessel of the hospital provided by the embodiment of the utility model with urine excretion body monofilm enrichment system
Structural schematic diagram;
Fig. 5 be hospital provided by the embodiment of the utility model with the excretion body of urine excretion body monofilm enrichment system collect from
The structural schematic diagram of heart pipe;
Fig. 6 is that hospital provided by the embodiment of the utility model is shown with the structure of the graduated cylinder of urine excretion body monofilm enrichment system
It is intended to;
Fig. 7 is structure of the hospital provided by the embodiment of the utility model with the sample bottle of urine excretion body monofilm enrichment system
Schematic diagram.
Specific embodiment
In order to make the purpose of the utility model, technical solutions and advantages more clearly understood, below in conjunction with attached drawing and implementation
Example, the present invention will be further described in detail.It should be appreciated that specific embodiment described herein is only used to explain
The utility model is not used to limit the utility model.
Referring to Fig. 1, a kind of hospital's urine excretion body monofilm enrichment is provided in one embodiment of the utility model
System 100, comprising:
The urine is carried out low-speed centrifugal processing and obtains supernatant by low speed centrifuge 10;
Filtration membrane filtration system 20, for filtering the impurity secreted except as noted other than body in the supernatant, described in enrichment
Excretion body, with 10 split settings of low speed centrifuge.
In this embodiment, hospital urine excretion body monofilm enrichment system 100, it is rich for being enriched with the excretion body
The excretion body collected can be used for diabetogenous nephrosis disease early diagnosis.The hospital includes with urine excretion body monofilm enrichment system 100
Low speed centrifuge 10 and filtration membrane filtration system 20, in the enrichment process of the urine excretion body, firstly, using low speed centrifuge
To remove the impurity such as cell fragment and bacterium in the urine, low speed centrifuge 10 is a kind of for 10 progress low-speed centrifugal processing
Common centrifuge, centrifugal force of the low speed centrifuge 10 by the sample for the urine being collected into 2000g carry out at room temperature
Low-speed centrifugal handles 30 minutes and generates the supernatant, is then collected the supernatant of generation;Referring to Fig. 7, preferably
, the supernatant of generation is contained in sample bottle 90, sample bottle 90 and 20 split settings of filtration membrane filtration system.Secondly,
According to the own physical property of the excretion body, the supernatant is put into filtration membrane filtration system 20 and carries out further mistake
Filter, filters and secretes external impurity in the supernatant except as noted, high degree ensure that the purity of the excretion body of enrichment.By
This, hospital urine excretion body monofilm enrichment system 100 does not need expensive laboratory apparatus (such as ultracentrifuge), preliminary to locate
Reason only needs common low speed centrifuge 10, and most of hospital can complete;It is big to handle sample size span, from 15ml~2000ml,
It can satisfy the needs of different sample size processing;Additional commercialization instrument and equipment is not needed, cost is saved;Using hydraulic
Dialysis principle, during the entire process of the hospital is enriched with urine excretion body with urine excretion body monofilm enrichment system 100, well
Maintain the physics and chemical characteristic of the excretion body itself.
Referring to fig. 2~Fig. 3, in one embodiment of the utility model, filtration membrane filtration system 20 includes:
Sample cell 30, upper part are holding tank 31, and lower part is the tubular conduit 32 for being connected to 31 bottom of holding tank, holding tank
31 positions for corresponding to tubular conduit 32 offer opening 33, and tubular conduit 32 is connected to by opening 33 with holding tank 31;
Filtration membrane 40, in a tubular form, the upper end are socketed on the outer wall of tubular conduit 32, and lower end is folded with film folder 50, passes through film
Folder 50 is closed its lower end.
In this embodiment, the supernatant is conveniently entered by sample cell 30 and is filtered in filtration membrane 40, the supernatant
Liquid pours into holding tank 31, and the supernatant enters tubular conduit 32 by opening 33, then naturally enters and is socketed on tubulose
In the filtration membrane 40 of the outer wall in channel 32, the lower end of filtration membrane 40 is closed by 50 folder of film folder, the preferably universal film folder of film folder 50
(Universal Closures).Filtration membrane 40 can be fastened by sealed membrane, and the sealed membrane specification is 2cmX6cm,
It is preferable to use sealed membranes described in two panels;The supernatant secretes external impurity by the filtering of filtration membrane 40 except as noted.
In one embodiment of the utility model, filtration membrane 40 is that molecular cut off is 1000KDa (kilodalton)
Filtration membrane, for retaining the excretion body;
The length of filtration membrane 40 is 30 centimetres.
In this embodiment, according to the own physical property of the excretion body, filtration membrane 40 is for molecular cut off
The nanometer film of 1000KDa (kilodalton) is filtered the supernatant by the filtration membrane that molecular cut off is 1000KDa, institute
Stating excretion body can be retained in filtration membrane 40, and when filtering to liquid remaining in filtration membrane 40 is 5 milliliters, this is remaining
Liquid is the outer liquid being enriched with, and is collected the enrichment process for completing outer liquid.Referring to Fig. 5, it is preferred that the hospital uses
Urine excretion body monofilm enrichment system 100 further includes that excretion body collects centrifuge tube 70, for holding the excretion body of the enrichment, with
20 split settings of filtration membrane filtration system, the capacity that excretion body collects centrifuge tube 70 are more than or equal to 5 milliliters, and excretion body is collected
Centrifuge tube 70 is preferably the sterile centrifuge tube for removing enzyme.Particularly, the length of filtration membrane 40 is 30 centimetres, and adapting to filtering needs
It asks.
In addition, the depth of holding tank 31 is greater than 20 centimetres, to meet the capacity requirement for the sample being filtered every time.
Referring to Fig. 3~Fig. 4, in one embodiment of the utility model, filtration membrane filtration system 20 further includes that waste liquid is received
Collect container 60, there is the waste collection chamber for holding waste liquid, when carrying out waste collection, waste liquid collection vessel 60 is socketed on sample cell
30 outside.
In this embodiment, the excretion body is enriched with by filtration membrane 40, and waste liquid then passes through filtration membrane 40 and oozes out, therefore in sample
The outside socket waste liquid collection vessel 60 in product pond 30 holds waste liquid, carries out waste collection.
Referring to Fig. 3~Fig. 4, in addition, the bottom of waste liquid collection vessel 60 is connected with pedestal 61, so that waste liquid collection vessel 60
Placement can be stablized.
In addition, waste liquid collection vessel 60 is in cylindrical, naturally it is also possible to be the other shapes such as cube shape;
The outer wall of waste liquid collection vessel 60 is made of transparent material, convenient for observing the collection situation of waste liquid, waste liquid is timely
Cleaning.
Referring to Fig. 6, in one embodiment of the utility model, hospital's urine excretion body monofilm enrichment system 100 is also
Including graduated cylinder 80, for measuring the liquid for needing predetermined amount to be used in the excretion body enrichment process, system is filtered with filtration membrane
It unites 20 split settings, graduated cylinder 80 is made of transparent material, and graduation mark 81 is provided on graduated cylinder 80, and the capacity of graduated cylinder 80 is
2000 milliliters.
Hospital's urine excretion body monofilm enrichment system 100, which is provided, using the utility model is enriched with the specific of urine excretion body
Method is as follows:
Embodiment 1:
(1) low-speed centrifugal: (hour) urine specimen for 24 hours of 1500~2000ml healthy volunteer is taken, is studied, low speed
Centrifuge 10 (AllegraTMX-22Centrifuge, Beckman Coulter), relative centrifugal force (RCF) 2000g, in room temperature
Under, 30 minutes, the impurity such as cell fragment, bacterium are removed, collect supernatant;
(2) it is filtered using the filtration membrane that molecular cut off is 1000KDa: collecting low-speed centrifugal treated supernatant, entirely
Portion is less than by being provided with the filtering of filtration membrane filtration system 20 for the filtration membrane that molecular cut off is 1000KDa with removing molecular weight
The urinary fractions of the excretion body, when liquid filtration is at 40 3~5cm of bottom of filtration membrane, the liquid is the outer of enrichment
Secrete body.
The filtration membrane filtration system 20 that uses when wherein filtration membrane 40 is dialysed, user assembles collocation various components when use,
Sample cell 30 1 are specifically included, 40 length of filtration membrane is 30cm, and universal film presss from both sides (Universal Closures) one, envelope
Membrana oralis (2cmX6cm) two panels.
Filtration membrane 40 purchased from bodyguard must pure (Shanghai) trade Co., Ltd Spectrum Labs Inc, filtration membrane 40 retention point
Son amount is 1000KDa, the excretion body of molecular cut off 1000KDa or more.
In conclusion the hospital of the utility model urine excretion body monofilm enrichment system, is set as including low-speed centrifugal
Machine and filtration membrane filtration system, low speed centrifuge are a kind of common centrifuges, do not need additional commercialization instrument and equipment,
Cost is saved, therefore, most of hospital can complete, and the urine is carried out low-speed centrifugal processing and obtains supernatant;Filtration membrane
Filtration system is used to filter except the impurity that excretion is external in the supernatant, with the low speed centrifuge split settings.According to outer
The own physical property for secreting body, is filtered by filtration membrane filtration system, and high degree ensure that the excretion body of enrichment
Purity and physicochemical property.Whereby, the hospital of the utility model is low in cost with urine excretion body monofilm enrichment system.
Certainly, the utility model can also have other various embodiments, without departing substantially from the spirit of the present invention and its essence
In the case of, those skilled in the art work as can make various corresponding changes and modifications, but these according to the utility model
Corresponding changes and modifications all should belong to the protection scope of the utility model the attached claims.
Claims (10)
1. a kind of hospital urine excretion body monofilm enrichment system characterized by comprising
The urine is carried out low-speed centrifugal processing and obtains supernatant by low speed centrifuge;
Filtration membrane filtration system is enriched with the excretion body for filtering the impurity secreted except as noted other than body in the supernatant,
With the low speed centrifuge split settings.
2. hospital according to claim 1 urine excretion body monofilm enrichment system, which is characterized in that the filtration membrane filtration
The system of mistake includes:
Sample cell, upper part are holding tank, and lower part is to be connected to the tubular conduit for accommodating trench bottom, and the holding tank is corresponding
Opening is offered in the position of the tubular conduit, the tubular conduit is connected to by the opening with the holding tank;
Filtration membrane, in a tubular form, the upper end are socketed on the outer wall of the tubular conduit, and lower end is folded with film folder, is pressed from both sides by the film
It is closed its lower end.
3. hospital according to claim 2 urine excretion body monofilm enrichment system, which is characterized in that the filtration membrane is
Molecular cut off is the filtration membrane of 1000KDa, for retaining the excretion body;
The length of the filtration membrane is 30 centimetres.
4. hospital according to claim 2 urine excretion body monofilm enrichment system, which is characterized in that the holding tank
Depth is greater than 20 centimetres.
5. hospital according to claim 2 urine excretion body monofilm enrichment system, which is characterized in that the filtration membrane filtration
The system of mistake further includes waste liquid collection vessel, has the waste collection chamber for holding waste liquid, and when carrying out waste collection, the waste liquid is received
Collection container is socketed on the outside of the sample cell.
6. hospital according to claim 5 urine excretion body monofilm enrichment system, which is characterized in that the waste collection
The bottom of container is connected with pedestal.
7. hospital according to claim 5 urine excretion body monofilm enrichment system, which is characterized in that the waste collection
Container is in cylindrical;
The outer wall of the waste liquid collection vessel is made of transparent material.
8. hospital according to claim 1 urine excretion body monofilm enrichment system, which is characterized in that hospital's urine
Liquid excretion body monofilm enrichment system further includes that excretion body collects centrifuge tube, for holding the excretion body of the enrichment, with the filter
Film filtration system split settings are crossed, the capacity that the excretion body collects centrifuge tube is more than or equal to 5 milliliters.
9. hospital according to claim 1 urine excretion body monofilm enrichment system, which is characterized in that hospital's urine
Liquid excretion body monofilm enrichment system further includes graduated cylinder, and with the filtration membrane filtration system split settings, the graduated cylinder is by transparent material
Matter is made, and graduation mark is provided on the graduated cylinder, and the capacity of the graduated cylinder is 2000 milliliters.
10. hospital according to claim 1 urine excretion body monofilm enrichment system, which is characterized in that the hospital uses
Urine excretion body monofilm enrichment system further includes sample bottle, described for holding with the filtration membrane filtration system split settings
The supernatant that low speed centrifuge generates.
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CN201821324206.XU CN209069692U (en) | 2018-08-16 | 2018-08-16 | Hospital's urine excretion body monofilm enrichment system |
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