CN208949311U - A kind of culture dish - Google Patents
A kind of culture dish Download PDFInfo
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- CN208949311U CN208949311U CN201821309653.8U CN201821309653U CN208949311U CN 208949311 U CN208949311 U CN 208949311U CN 201821309653 U CN201821309653 U CN 201821309653U CN 208949311 U CN208949311 U CN 208949311U
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- culture dish
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Abstract
The purpose of the utility model is to provide a kind of culture dishes.Culture dish includes bottom plate and the side plate from bottom plate extension, and the upper surface of bottom plate and the inner surface of side plate are enclosed the accommodation space for holding cell；Bottom plate has the first cross scale, the second cross scale, third cross scale, the 4th cross scale and the 5th cross scale；Second cross scale, third cross scale, the 4th cross scale and the 5th cross scale are configured to be located at four apexes of the same square on bottom plate, and the first cross scale is configured to positioned at the center of square.Since bottom plate has the first cross scale, second cross scale, third cross scale, the 4th cross scale and the 5th cross scale, so that carry out counting process to cell directly just can be carried out in culture dish without using cell counting board, therefore it is easy to count cell.
Description
Technical field
The utility model relates to a kind of culture dishes.
Background technique
Cell clone technology is also in cell culture, and for entire biotechnology, cell culture is one essential
Process, the extensive clone of cell culture inherently cell.Cell culture technology can be by a cell by a large amount of trainings
It forms as simple many cells that are unicellular or seldom breaking up, this is the essential link of clone technology, and cell culture
The inherently clone of cell.
Culture dish is a kind of labware for cell culture, bottom and a lid group by flat circle plate-like
At being generally made of glass or plastics.At present the common Tissue Culture Dish specification in laboratory be usually 35mm, 60mm and
100mm, these culture dishes can only meet the custom requirements such as cell culture amplification, and for cell count, observation specific cells point
It splits migration and single cell clone positioning etc. and generally requires external auxiliary tool.Such as cell count, it is generally the case that experimenter
Concentration of cell suspension can be measured with cell counting board in advance, then be inoculated in routine culture ware, but after cultivating a period of time, carefully
After born of the same parents divide amplification, cell number in culture dish at this time can not be accurately known, it is necessary to number is surveyed with tally again after digestion, this
Sample increases unnecessary experimental procedure, increases the risk of cell contamination.
Further, since cell is numerous in culture dish, number is often difficult to find a certain specific cells simultaneously with 100,000, Bai Wanji
Its division growth is observed continuously, and after this specific cells continuously divides for several times, is formed by monoclonal cell group
Also it is difficult to position, increases the difficulty of operation.
This field needs a kind of culture dish for being easy to count cell.
Utility model content
The purpose of this utility model is to provide a kind of culture dish, have the advantages that be easy to count cell.
For the culture dish for realizing the purpose, including bottom plate and the side plate extended from the bottom plate, the upper table of the bottom plate
The inner surface of face and the side plate is enclosed the accommodation space for holding cell；
The bottom plate has the first cross scale, the second cross scale, third cross scale, the 4th cross scale and the 5th
Cross scale；
The second cross scale, the third cross scale, the 4th cross scale and the 5th cross scale
It is configured to be located at four apexes of the same square on the bottom plate, the first cross scale is configured to
Positioned at the center of the square.
The culture dish, further feature are that the shape of the bottom plate is circle；The center of the square with
The center of the bottom plate is overlapped.
The culture dish, further feature be, the first cross scale, the second cross scale, described
The range of third cross scale, the 4th cross scale and the 5th cross scale is ﹣ 0.5mm to+0.5mm, scale division value
For 0.01mm.
The culture dish, further feature are that the bottom plate is distinguished on two diagonal lines of the square
With the first scale and the second scale；The range of first scale and second scale is ﹣ 10mm to+10mm, scale division value
For 0.5mm.
The culture dish, further feature are that the bottom plate is made of clear material；First scale, institute
The second scale, the first cross scale, the second cross scale, the third cross scale, the 4th cross is stated to carve
Degree and the 5th cross scale are embedded in the bottom plate.
The culture dish, further feature are that the bottom plate is made of clear material；First scale, institute
The second scale, the first cross scale, the second cross scale, the third cross scale, the 4th cross is stated to carve
Degree and the 5th cross scale are formed in the upper surface or lower surface of the bottom plate.
The culture dish, further feature are that the diameter of the bottom plate is 35mm, and the height of the side plate is
10mm。
The culture dish, further feature are that the transparent material is transparent glass material or transparent plastic
Material.
The culture dish, further feature are the upper surface of the bottom plate or lower surface recess or raised,
To form first scale, second scale, the first cross scale, the second cross scale, the described 30th
Word scale, the 4th cross scale and the 5th cross scale.
The culture dish, further feature be, the first cross scale, the second cross scale, described
Third cross scale, the 4th cross scale and the 5th cross scale all have orthogonal two articles of scales.
The positive effect of the utility model is: culture dish provided by the utility model, including bottom plate and from bottom plate
The side plate of extension, the upper surface of bottom plate and the inner surface of side plate are enclosed the accommodation space for holding cell；Bottom plate has
First cross scale, the second cross scale, third cross scale, the 4th cross scale and the 5th cross scale；Second cross is carved
Degree, third cross scale, the 4th cross scale and the 5th cross scale are configured to be located at the same pros on bottom plate
Four apexes of shape, the first cross scale are configured to positioned at the center of square.
Culture dish provided by the utility model, since bottom plate has the first cross scale, the second cross scale, third cross
Scale, the 4th cross scale and the 5th cross scale；And the second cross scale, third cross scale, the 4th cross scale and
5th cross scale is configured to be located at square four apex on bottom plate, and the first cross scale is set
At the center for being located at square, so that carrying out counting process without using cell counting board, directly in culture dish just to cell
It can be carried out, therefore be easy to count cell.
Detailed description of the invention
The above and other feature, property and advantage of the utility model will be by with reference to the accompanying drawings and examples
Description and become readily apparent from, in which:
Fig. 1 is the schematic diagram of culture dish in the utility model；
Fig. 2 is the top view of culture dish in the utility model；
Fig. 3 is the first cross scale schematic diagram in the utility model.
Specific embodiment
The utility model is described in further detail with attached drawing combined with specific embodiments below, elaborates in the following description
More details are in order to fully understand the utility model, but the utility model obviously can be different from what this was described with a variety of
Other way is implemented, and those skilled in the art can be without prejudice to the utility model connotation according to practical application feelings
Condition makees similar popularization, deduces, therefore should not limit the protection scope of the utility model with the content of this specific embodiment.
It should be noted that
Fig. 1 to Fig. 3, and should not be in this, as only as an example, it is drawn according to the condition of equal proportion
The protection scope of the utility model actual requirement is construed as limiting.
As shown in Figure 1, culture dish includes that bottom plate 1 and the side plate 2 from the extension of bottom plate 1, side plate 2 form a circle, cylinder is formed
The culture dish of shape.The upper surface of bottom plate 1 and the inner surface of side plate 2 are enclosed the accommodation space for holding cell.
In a comparative example, experimenter can measure concentration of cell suspension with cell counting board in advance, then be inoculated in often
Culture dish is advised, after cell cultivates a period of time in culture dish, since cell division expands, causes accurately know at this time
Cell number in culture dish, it is necessary to survey number with tally again after digestion, which adds unnecessary experimental procedures, increase
The risk of cell contamination.
To solve the above problems, cell technology is made to become to be more easier, disclosed in one embodiment of the utility model
A kind of culture dish, as shown in Figure 1, 2, the bottom plate 1 of culture dish have the first cross scale 11, the second cross scale the 12, the 30th
Word scale 13, the 4th cross scale 14 and the 5th cross scale 15；Second cross scale 12, third cross scale the 13, the 40th
Word scale 14 and the 5th cross scale 15 are configured to be located at four apexes of the same square 1a on bottom plate 1,
First cross scale 11 is configured to positioned at the center of square 1a.
Since bottom plate 1 has the first cross scale 11, the second cross scale 12, third cross scale 13, the 4th cross are carved
Degree 14 and the 5th cross scale 15；And the second cross scale 12, third cross scale 13, the 4th cross scale 14 and the 50th
Word scale 15 is configured to four apexes of be located on bottom plate 1 square 1a, and the first cross scale 11 is set
It is set to the center positioned at square 1a, so that the process counted to cell is directly being cultivated without using cell counting board
It just can be carried out in ware, therefore be easy to count cell.Using culture dish disclosed in the embodiments of the present invention to thin
The method that born of the same parents are counted will be described later.
Continue to refer to figure 1,2, the shape of bottom plate 1 is circle；The center of square 1a is overlapped with the center of bottom plate 1.
As shown in figure 3, the range of the first cross scale 11 is ﹣ 0.5mm extremely+0.5mm, scale division value 0.01mm.10th
The numerical value of tested amount representated by the minimum lattice of word scale 11 is called scale division value, i.e. minimum scale, and the minimum scale is identified
In Fig. 3.Second cross scale 12, third cross scale 13, the 4th cross scale 14 and the 5th cross scale 15 shape with
First cross scale 11 is identical, therefore can also refer to Fig. 3.
Continue to refer to figure 1,2, bottom plate 1 is respectively provided with the first scale 16 and the second quarter on two diagonal lines of square 1a
Degree 17；The range of first scale 16 and the second scale 17 is ﹣ 10mm extremely+10mm, scale division value 0.5mm.In Fig. 1, the first quarter
Graduation mark on degree 16 and the second scale 17 only depicts a part.First scale 16 and the effect of the second scale 17 are to monoclonal
Cell mass is positioned.
On the setting position of scale, in one embodiment, bottom plate 1 is made of clear material；First scale 16, second
Scale 17, the first cross scale 11, the second cross scale 12, third cross scale 13, the 4th cross scale 14 and the 5th cross
Scale 15 is embedded in bottom plate 1.
In another embodiment, bottom plate 1 is made of clear material；First scale 16, the second scale 17, the first cross are carved
The 11, second cross scale 12 of degree, third cross scale 13, the 4th cross scale 14 and the 5th cross scale 15 are formed in bottom plate 1
Upper surface or lower surface.More specifically, the upper surface of bottom plate 1 or lower surface recess or protrusion, to form for the first quarter
Spend the 16, second scale 17, the first cross scale 11, the second cross scale 12, third cross scale 13,14 and of the 4th cross scale
5th cross scale 15.
In one embodiment, artistic carving can be used to be formed in the upper surface of bottom plate 1 or lower surface in recess.Protrusion
Printing technology can be used to be formed in the upper surface of bottom plate 1 or lower surface.
In other are implemented, the diameter of bottom plate 1 is 35mm, and the height of side plate 2 is 10mm.Transparent material is transparent glass
Material or transparent plastic material.
As shown in figure 3, the first cross scale 11, the second cross scale 12, third cross scale 13, the 4th cross scale 14
Orthogonal two articles of scales are all had with the 5th cross scale 15.
The practical approach of the culture dish in the utility model embodiment is described below:
A. cell count: in culture dish after inoculating cell, standing 20min, according to cell size 40 × or 100 ×
Microscopically observation cell.The first cross scale 11, the second cross scale 12, third cross scale 13, the 4th cross is counted to carve
Cell in the region 0.5mm × 0.5mm of degree 14 and the 5th cross scale 15, is averaged x, then counts according to the following formula
Total number of cells y in culture dish.Wherein, culture dish inner cell sum
X is the first cross scale 11, the second cross scale 12, third cross scale 13, the 4th cross scale 14 and the 5th
The average of the interior cell for five counting regions that cross scale 15 is respectively provided with.For embodiment shown in Fig. 3, count
The area in region is 0.5mm × 0.5mm=0.25mm2.In above-mentioned formula, the diameter of bottom plate 1 is 35mm, the area of bottom plate 1
For 962mm2。
It, still can be according to total number of cells y in the method count plates after cell culture expands for a period of time.
More universally, above-mentioned formula can be summarized as
Wherein, x is the first cross scale 11, the second cross scale 12, third cross scale 13,14 and of the 4th cross scale
The average of the interior cell for five counting regions that 5th cross scale 15 is respectively provided with.S is the area of bottom plate 1, and A is meter
The area in number region.Y is the total number of cells in culture dish.
B. observation specific cells division migration: cell inoculation after this culture dish, it is preferential select the first cross scale 11,
Cell at second cross scale 12, third cross scale 13, the 4th cross scale 14 and the 5th cross scale 15 is used as and needs to see
The specific cells examined.After choosing specific cells, by above-mentioned five cross calibration records cell coordinate, so that next energy is quasi-
Really find same cell.It is selected at the time point being observed continuously, to take pictures under microscope according to cell strain type, compares various time points
The image clapped, analyzes the division migration of cell, and accurately calculates cell migration distance according to reference axis scale.
C. single cell clone positions: after cell transfecting, screening antibiotic is added, after a period of time, with the thin of resistant gene
Born of the same parents will form monoclonal cell group.It in this culture dish, finds certain and transfects successful cell, pass through above-mentioned first scale 16 and the
Two scales 17 record coordinate, are observed continuously until the monoclonal formed.
Although the utility model is disclosed as above with preferred embodiment, its be not for limiting the utility model, it is any
Those skilled in the art without departing from the spirit and scope of the utility model, can make possible variation and modification, all
It is the content without departing from technical solutions of the utility model, made by the above technical examples according to the technical essence of the present invention
What modification, equivalent variations and modification, each falls within the protection scope that the utility model claims are defined.
Claims (10)
1. a kind of culture dish, including bottom plate (1) and the side plate (2) extended from the bottom plate (1), the upper surface of the bottom plate (1)
The accommodation space for holding cell is enclosed with the inner surface of the side plate (2)；
It is characterized in that, the bottom plate (1) has the first cross scale (11), the second cross scale (12), third cross scale
(13), the 4th cross scale (14) and the 5th cross scale (15)；
The second cross scale (12), the third cross scale (13), the 4th cross scale (14) and the described 5th
Cross scale (15) is configured to be located at four apexes of same square (1a) on the bottom plate (1), described
First cross scale (11) is configured to be located at the center of square (1a).
2. culture dish as described in claim 1, which is characterized in that the shape of the bottom plate (1) is circle；The square
The center of (1a) is overlapped with the center of the bottom plate (1).
3. culture dish as described in claim 1, which is characterized in that the first cross scale (11), second cross are carved
Spend the range of (12), the third cross scale (13), the 4th cross scale (14) and the 5th cross scale (15)
For ﹣ 0.5mm to+0.5mm, scale division value 0.01mm.
4. culture dish as described in claim 1, which is characterized in that the bottom plate (1) is right at two of square (1a)
The first scale (16) and the second scale (17) are respectively provided on linea angulata；First scale (16) and second scale (17)
Range is ﹣ 10mm extremely+10mm, scale division value 0.5mm.
5. culture dish as claimed in claim 4, which is characterized in that the bottom plate (1) is made of clear material；First quarter
Spend (16), second scale (17), the first cross scale (11), the second cross scale (12), the described 30th
Word scale (13), the 4th cross scale (14) and the 5th cross scale (15) are embedded in the bottom plate (1).
6. culture dish as claimed in claim 4, which is characterized in that the bottom plate (1) is made of clear material；First quarter
Spend (16), second scale (17), the first cross scale (11), the second cross scale (12), the described 30th
Word scale (13), the 4th cross scale (14) and the 5th cross scale (15) are formed in the upper table of the bottom plate (1)
Face or lower surface.
7. culture dish as claimed in claim 2, which is characterized in that the diameter of the bottom plate (1) is 35mm, the side plate (2)
Height be 10mm.
8. such as culture dish described in claim 5 or 6, which is characterized in that the transparent material is for transparent glass material or thoroughly
Bright plastic material.
9. culture dish as claimed in claim 6, which is characterized in that the upper surface of the bottom plate (1) or lower surface recess or
Person's protrusion, to form first scale (16), second scale (17), the first cross scale (11), described second
Cross scale (12), the third cross scale (13), the 4th cross scale (14) and the 5th cross scale (15).
10. culture dish as described in claim 1, which is characterized in that the first cross scale (11), second cross are carved
Degree (12), the third cross scale (13), the 4th cross scale (14) and the 5th cross scale (15) all have
Orthogonal two scales.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821309653.8U CN208949311U (en) | 2018-08-14 | 2018-08-14 | A kind of culture dish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821309653.8U CN208949311U (en) | 2018-08-14 | 2018-08-14 | A kind of culture dish |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208949311U true CN208949311U (en) | 2019-06-07 |
Family
ID=66733656
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821309653.8U Active CN208949311U (en) | 2018-08-14 | 2018-08-14 | A kind of culture dish |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208949311U (en) |
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2018
- 2018-08-14 CN CN201821309653.8U patent/CN208949311U/en active Active
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Address after: 200032 Shanghai Xuhui District Xietu Road No. 2140 Patentee after: Shanghai Institute of biomedical technology Address before: 200032 Shanghai Xuhui District Xietu Road No. 2140 Patentee before: SHANGHAI INSTITUTE OF PLANNED PARENTHOOD RESEARCH |