CN208949290U - A kind of slow virus cell strain cryovial - Google Patents
A kind of slow virus cell strain cryovial Download PDFInfo
- Publication number
- CN208949290U CN208949290U CN201821279096.XU CN201821279096U CN208949290U CN 208949290 U CN208949290 U CN 208949290U CN 201821279096 U CN201821279096 U CN 201821279096U CN 208949290 U CN208949290 U CN 208949290U
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- Prior art keywords
- screw thread
- lid
- wall
- elastomeric pad
- annular groove
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A kind of slow virus cell strain cryovial, including tube body and Guan Gai, tube body and Guan Gai are connected through a screw thread, hollow out band is equipped in the middle part of the tube body, hollow out band inner wall and tube wall surround Sheng sap cavity, are equipped with the first internal screw thread at body open, tube wall is circumferentially arranged with anti-skid bulge;The pipe lid includes lid, elastomeric pad and fixes sleeve, the lid bottom is provided with annular groove, cell wall is equipped with the first external screw thread on the inside of annular groove, annular groove bottom is equipped with seal washer, and lid outer wall is equipped with the second external screw thread, and elastomeric pad is placed at the top of lid, annular groove a is provided at the top of the elastomeric pad, fixes sleeve inner wall is equipped with the second internal screw thread, downward annular protrusion is equipped at the top of fixes sleeve, annular protrusion is in the annular groove a of elastomeric pad.The cryovial can be realized quick-thawing and bring back to life cell in pipe, pipe cover can draught head inside and outside balance pipe, the anti-skid bulge of tube wall is convenient for tightening and unscrew pipe lid.
Description
Technical field
The utility model relates to biological Cryopreservation Technology fields, and in particular to a kind of slow virus cell strain cryovial.
Background technique
Cell cryopreservation is one of the main method that cell saves.Cell is placed in using Cryopreservation Technology low in -196 DEG C of liquid nitrogen
Temperature saves, and cell can be made to be temporarily disengaged from growth conditions and save its cell characteristics, multiple again when needed in this way
Soviet Union's cell is for testing.Cell cryopreservation and the basic principle of recovery are that slow freeze is melted fastly, experiments have shown that in this way can be maximum
Save cell viability.
If thawing time is too long in resuscitation process, it will lead to low temperature and cell damaged, it is serious to may cause
Recovery of thawing fails, and the cell of preservation is elaborated in loss, and existing cryovial is mostly cylindric, when thawing close to tube wall
Cell is first recovered and the cell at center does not thaw also, if the cell that overlong time will lead to close tube wall loses activity, patent
CN205071975U discloses a kind of cryopreservation tube of quick-thawing, by the extrusion that connects on sealing cover by cryocoagulation state
Cell mixture from cryopreservation tube pull out be put into quick-thawing in 37 ° of solution with culture medium, this patent is asked there are following
Topic: the cell mixture of curdled appearance is difficult to pull out from pipe, even if will also result in during being put into culture medium after pulling out
Cell is contaminated.Patent CN206866484U discloses a kind of Human Active's immunocyte cryopreservation tube, at bottom center
It is cecum at the top of extension with the extension extended straight up, bottom is opening, which is freezing and thawing
Freezing liquid and thawing solution cannot flow into extension in journey, cannot play quick-thawing resurrection well.And cryovial is freezing
Internal rising-heat contracting-cold during depositing and thawing, air pressure, which can change, may cause freezing tracheal rupture.Although existing cryovial
It is all covered in pipe and is equipped with the striped for increasing friction, but tube body is mostly smooth outer wall, is not easy to experimenter and tightens and unscrew pipe
Lid.
Utility model content
The utility model to solve the above problems, devises a kind of slow virus cell strain cryovial, the freezing
Pipe can be realized quick-thawing and bring back to life cell in pipe, can draught head inside and outside balance pipe and convenient for tightening and unscrewing pipe lid,
The anti-skid bulge of tube wall is convenient for tightening and unscrewing pipe lid.
In order to achieve the above technical purposes, reach above-mentioned technical effect, the utility model is real by the following technical programs
Existing:
A kind of slow virus cell strain cryovial, including tube body and Guan Gai, tube body and Guan Gai are connected through a screw thread, the tube body
Middle part is equipped with hollow out band, and hollow out band inner wall and tube wall surround Sheng sap cavity, be equipped with the first internal screw thread at body open, bottom is opened
There is circular groove, tube wall is circumferentially arranged with anti-skid bulge;
The pipe lid includes lid, elastomeric pad and fixes sleeve, and the lid is cylindrical shape, and lid bottom is provided with annular
Groove, annular groove inside cell wall are equipped with the first external screw thread being adapted to the first internal screw thread, and annular groove bottom is equipped with gasket
Circle, lid outer wall are equipped with the second external screw thread, and elastomeric pad is placed at the top of lid, are provided with annular groove a at the top of the elastomeric pad,
Fixes sleeve inner wall is equipped with the second internal screw thread being adapted to the second external screw thread, is equipped with downward annular protrusion at the top of fixes sleeve,
Annular protrusion is in the annular groove a of elastomeric pad.
Further, the tube wall is equipped with containing mark line.
Further, the circular groove bottom is pasted with two dimensional code.
Further, the fixes sleeve outer wall is handled using cancellous screw thread.
Further, the elastomeric pad uses silica gel material.
Further, the long side of the rectangular through-hole is axial parallel with tube body.
The beneficial effects of the utility model are: being equipped with hollow out band in the middle part of the cryovial, it can be realized quick-thawing and bring back to life pipe
Interior cell, pipe cover end elastomeric pad can draught head inside and outside balance pipe, the anti-skid bulge of tube wall is convenient for tightening
With unscrew pipe lid.
Detailed description of the invention
In order to illustrate more clearly of the technical solution of the utility model embodiment, make required for being described below to embodiment
Attached drawing is briefly described, it should be apparent that, the drawings in the following description are merely some embodiments of the present invention,
For those of ordinary skill in the art, without creative efforts, it can also be obtained according to these attached drawings
Other attached drawings.
Fig. 1 is a kind of external structure schematic diagram of slow virus cell strain cryovial;
Fig. 2 is the cross-sectional view of pipe lid structure;
Fig. 3 is the cross-sectional view of pipe structure;
Fig. 4 is the top view of elastomeric pad.
In attached drawing, parts list represented by the reference numerals are as follows:
1- tube body, 11- hollow out band, 12- anti-skid bulge, the first internal screw thread of 13-, 14- circular groove, 15- two dimensional code, 16-
Containing mark line, 2- fixes sleeve, the second internal screw thread of 21-, 22- annular protrusion, 3- lid, the second external screw thread of 31-, 32- sealing
Washer, the first external screw thread of 33-, 4- elastomeric pad, 41- annular groove a.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without creative efforts
All other embodiment obtained, fall within the protection scope of the utility model.
Refering to fig. 1 shown in-Fig. 4, a kind of slow virus cell strain cryovial, including tube body 1 and Guan Gai, tube body 1 and pipe lid are logical
Threaded connection is crossed, hollow out band 11 is equipped in the middle part of the tube body 1,11 inner wall of hollow out band and tube wall surround Sheng sap cavity, bring back to life thawing
When cell in cryovial, thawing solution can pass through hollow out band 11, keep pipe inside and outside cell heated simultaneously, be convenient for pipe inner cell fast
Speed, which is thawed, brings back to life, and 1 opening of tube body is equipped with the first internal screw thread 13, and 1 bottom of tube body is provided with circular groove 14,14 bottom of circular groove
It is pasted with two dimensional code 15, two dimensional code has small in size, and information memory capacity is big, and code reader editor can be used and read information, use
Convenient, 1 outer wall of tube body cooperates the antiskid thread of 2 outer wall of pipe lid fixes sleeve circumferentially arranged with anti-skid bulge 12, anti-skid bulge 12,
Personnel easy to use tighten and unscrew pipe lid;
The pipe lid includes lid 3, elastomeric pad 4 and fixes sleeve 2, and the lid 3 is cylindrical shape, and 3 bottom of lid is opened
There is annular groove, cell wall is equipped with the first external screw thread 33 being adapted to the first internal screw thread 13, annular groove bottom on the inside of annular groove
Equipped with seal washer 32,3 outer wall of lid is equipped with the second external screw thread 31, and elastomeric pad 4 is placed in 3 top of lid, the elastomeric pad 4
Top is provided with annular groove a41, and 2 inner wall of fixes sleeve is equipped with the second internal screw thread 21 being adapted to the second external screw thread 31, fixing sleeve
Cylinder 2 is fixed on outside lid 3 by the second internal screw thread 21 and the second external screw thread 31, and downward annular is equipped at the top of fixes sleeve 2
Protrusion 22, annular protrusion 22 are tightly secured in 3 top of lid in the annular groove a41 of elastomeric pad 4, by elastomeric pad 4,
When cryovial expands with heat and contract with cold, and air pressure inside changes, elastomeric pad 4 can be recessed and heave with the air pressure inside and outside equilibrium freezing pipe
Difference.
Therein, 1 outer wall of tube body is equipped with containing mark line 16, convenient for observing the capacity of cryovial inner cell.
Therein, 2 outer wall of fixes sleeve is handled using cancellous screw thread, increases the roughness of 2 outer wall of fixes sleeve.
Therein, the elastomeric pad 4 uses silica gel material, and silica gel material has good elasticity and has with tissue
Excellent compatibility.
The concrete application of the utility model are as follows: use mobile phone or the two dimensional code 15 of two dimensional code code reader scanning freezing bottom of the tube
Information editing is carried out, lower tube cap is then twisted, the slow virus cell strain for needing freezen protective and protection reagent are put into togerther freezing
In pipe, the antiskid thread in the anti-skid bulge 12 and fixes sleeve 2 of 1 outer wall of tube body, will be cold convenient for pipe lid to be tightened on tube body 1
Frozen pipe carries out freezen protective, and in freezing, intraductal atmospheric pressure is reduced, and the recess of elastomeric pad 4 refrigerates the inside and outside draught head of pipe to balance,
It when needing to carry out cell defrosting resurrection, takes out cryovial and is immediately placed in 37 ° of warm water, the hollow out band 11 at 1 middle part of tube body can fill
Full warm water, brings back to life convenient for pipe inner cell quick-thawing, and intraductal atmospheric pressure increases in course of defrosting, and elastomeric pad 4 can be heaved with flat
Pressure difference inside and outside weighing apparatus tube body 1.The cryovial can quick-thawing bring back to life pipe inner cell, pressure difference inside and outside equilibrium freezing pipe, just
In tightening and unscrew pipe lid.
In the description of this specification, the description of reference term " one embodiment ", " example ", " specific example " etc. means
Particular features, structures, materials, or characteristics described in conjunction with this embodiment or example are contained at least one of the utility model
In embodiment or example.In the present specification, schematic expression of the above terms be not necessarily referring to identical embodiment or
Example.Moreover, particular features, structures, materials, or characteristics described can be in any one or more embodiment or examples
In can be combined in any suitable manner.
The preferred embodiment in the utility model disclosed above is only intended to help to illustrate the utility model.Preferred embodiment is simultaneously
There is no the details that detailed descriptionthe is all, also not limiting the utility model is only the specific embodiment.Obviously, according to this theory
The content of bright book can make many modifications and variations.These embodiments are chosen and specifically described to this specification, is in order to preferably
The principles of the present invention and practical application are explained, so that skilled artisan be enable to better understand and utilize this
Utility model.The utility model is limited only by the claims and their full scope and equivalents.
Claims (5)
1. a kind of slow virus cell strain cryovial, including tube body and Guan Gai, tube body and Guan Gai are connected through a screw thread, feature exists
In:
Hollow out band is equipped in the middle part of the tube body, hollow out band inner wall and tube wall surround Sheng sap cavity, be equipped with spiral shell in first at body open
Line, bottom are provided with circular groove, and tube wall is circumferentially arranged with anti-skid bulge;
The pipe lid includes lid, elastomeric pad and fixes sleeve, and the lid is cylindrical shape, and it is recessed that lid bottom is provided with annular
Slot, annular groove inside cell wall are equipped with the first external screw thread being adapted to the first internal screw thread, and annular groove bottom is equipped with seal washer,
Lid outer wall is equipped with the second external screw thread, and elastomeric pad is placed at the top of lid, is provided with annular groove a at the top of the elastomeric pad, Gu
Determine sleeve lining and be equipped with the second internal screw thread being adapted to the second external screw thread, is equipped with downward annular protrusion, ring at the top of fixes sleeve
Shape protrusion is in the annular groove a of elastomeric pad.
2. a kind of slow virus cell strain cryovial according to claim 1, it is characterised in that: the tube wall, which is equipped with, to be held
Measure graduation mark.
3. a kind of slow virus cell strain cryovial according to claim 1, it is characterised in that: the circular groove bottom is viscous
Post two dimensional code.
4. a kind of slow virus cell strain cryovial according to claim 1, it is characterised in that: the fixes sleeve outer wall is adopted
It is handled with cancellous screw thread.
5. a kind of slow virus cell strain cryovial according to claim 1, it is characterised in that: the elastomeric pad uses silicon
Glue material.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201821279096.XU CN208949290U (en) | 2018-08-09 | 2018-08-09 | A kind of slow virus cell strain cryovial |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201821279096.XU CN208949290U (en) | 2018-08-09 | 2018-08-09 | A kind of slow virus cell strain cryovial |
Publications (1)
Publication Number | Publication Date |
---|---|
CN208949290U true CN208949290U (en) | 2019-06-07 |
Family
ID=66733366
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201821279096.XU Expired - Fee Related CN208949290U (en) | 2018-08-09 | 2018-08-09 | A kind of slow virus cell strain cryovial |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN208949290U (en) |
-
2018
- 2018-08-09 CN CN201821279096.XU patent/CN208949290U/en not_active Expired - Fee Related
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Date | Code | Title | Description |
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GR01 | Patent grant | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190607 Termination date: 20200809 |
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CF01 | Termination of patent right due to non-payment of annual fee |