CN208917207U - A kind of biology 3 D-printing environment real time monitoring and active maintenance device - Google Patents
A kind of biology 3 D-printing environment real time monitoring and active maintenance device Download PDFInfo
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- CN208917207U CN208917207U CN201821269314.1U CN201821269314U CN208917207U CN 208917207 U CN208917207 U CN 208917207U CN 201821269314 U CN201821269314 U CN 201821269314U CN 208917207 U CN208917207 U CN 208917207U
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- 238000007639 printing Methods 0.000 title claims abstract description 43
- 238000012544 monitoring process Methods 0.000 title claims abstract description 22
- 238000012423 maintenance Methods 0.000 title claims abstract description 16
- 239000007788 liquid Substances 0.000 claims abstract description 86
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 70
- 238000000465 moulding Methods 0.000 claims abstract description 56
- 238000012258 culturing Methods 0.000 claims abstract description 54
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- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 36
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 27
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 26
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 2
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Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a kind of biological 3 D-printing environment real time monitoring and active maintenance device, chamber is maintained including the double-deck maintaining liquid supply chamber and molding, the bilayer maintaining liquid supply chamber includes the heating room positioned at the culturing room on upper layer and positioned at lower layer, maintaining liquid is equipped in the culturing room, heating is indoor to be equipped with heat transfer medium, the external carbon dioxide bottle of the culturing room and oxygen cylinder, the culturing room and molding maintain chamber side to be equipped with two-way peristaltic pump, and two-way peristaltic pump, culturing room maintain chamber to be successively connected to form maintaining liquid circulation path from beginning to end with molding.During biological 3 D-printing and in biological tissue's incubation in printing later period, the environmental condition of maintaining liquid monitors automatically by control system and controls realization, regulate and control to keep the stabilization of culture solution and its ambient enviroment by micro controller unit, contaminated possibility is also reduced simultaneously, repetitive operation is higher, and experimental result is relatively reliable.
Description
Technical field
The utility model relates to biometric print technical field, especially a kind of biological 3 D-printing environment monitors in real time and lives
Property maintain device.
Background technique
Organizational project is application project, the principle of life science and method to prepare biologically active artificial substituting
Object, to maintain, restore or improve part or all of function of tissue, organ.Organizational project can be applied to include bone
With cartilage tissue engineered, skin tissue engineering, the various aspects such as the visceral organ tissues such as the heart, liver, kidney.
Biological 3D printing be using 3D printing it is discrete/basic principle and method of accumulation molding, to biomaterial (including day
Right biomaterial and synthetic polymer) or cell suspending liquid carry out controlled printing, being formed required has bioactivity
Implantation material, cell three-dimensional structure or artificial organ organ etc..Biological 3D printing is handed over as life science and Modern Manufacturing Science
The emerging technology of fork is conducive to construct various three-dimensional bionic structures needed for organizational project.
But according to the biological 3 D-printing device of existing biological three-dimensional printing technology research and development, there are deficiencies below
Place:
1, biological 3 D-printing device does not account for maintaining the printing environment of chamber in molding in print procedure, is usually out
It puts formula to print, there are harmful components, various viruses and anaphylactogen in air, the biology of biological 3 D-printing product will be directly affected
Activity.Also there is part super-clean bench to can control temperature, but be unable to control carbon dioxide and oxygen content.Also it can not replace in real time
Nutrient solution needed for tissue growth.
2, it after printing shaping, needs to carry out histiocytic culture, needs to keep stable environmental condition at this time, such as maintain
Temperature, pH value and partial pressure of oxygen of liquid etc..In the incubation of the prior art, it need to repeat to open to maintain chamber, at regular time and quantity more
It changes cell maintenance medium and monitors the variation of its ambient enviroment, the finished product of biological 3 D-printing is essentially due to potential pollution and not
Stable temperature loses bioactivity in undesirable growing environment.
Utility model content
Purpose of utility model: technical problem to be solved in the utility model is in view of the deficiencies of the prior art, to provide one
The biological 3 D-printing environment real time monitoring of kind and activity maintenance device.
In order to solve the above-mentioned technical problem, the utility model provides a kind of biological 3 D-printing environment real time monitoring and living
Property maintain device, maintain chamber including the double-deck maintaining liquid supply chamber and the molding that is placed in super-clean bench, the bilayer maintaining liquid supplies
It include being located at the culturing room on upper layer and being equipped with maintaining liquid in the heating room of lower layer, the culturing room to room, heating interior is set
There is a heat transfer medium, the external carbon dioxide bottle of the culturing room and oxygen cylinder, it is double that the culturing room and molding maintain chamber side to be equipped with
To peristaltic pump, two-way peristaltic pump, culturing room maintain chamber to be successively connected to form maintaining liquid circulation path from beginning to end with molding.By two-way compacted
The maintaining liquid circulation path that dynamic pump is guided and controlled, realizes the function of printing environment activity maintenance.
In the utility model, temperature sensor is equipped in the culturing room, temperature sensor is electrically connected to microcontroller.It is micro-
Controller connects and controls temperature sensor and carbon dioxide sensor, realizes molding and chamber maintaining liquid ingredient is maintained to supervise in real time
The function of control.
In the utility model, it is equipped with heater in the heat transfer medium of the heating room, heater includes overheating protection circuit.
In the utility model, the carbon dioxide bottle is connected to CO2 flow controller, and oxygen cylinder is communicated to oxygen flux control device,
CO2 flow controller and oxygen flux control device are communicated to high efficiency particle air filter, high efficiency particle air filter connection jointly
To culturing room；The CO2 flow controller and oxygen flux control device are each coupled electrically to microcontroller.
In the utility model, the two-way peristaltic pump arrival end connection connection maintains liquid container and media Containers, two-way
Peristaltic pump outlet end is respectively communicated with culturing room and molding maintains chamber.
In the utility model, the culturing room is sealed set.
In the utility model, the media Containers include maintaining liquid container, maintain to be equipped with maintaining liquid in liquid container, two-way compacted
Dynamic pump will maintain the maintaining liquid in liquid container to be transported in culturing room, and maintaining liquid is transported to molding and maintains chamber, molding in culturing room
Intracavitary maintaining liquid is maintained to flow back into two-way peristaltic pump.Maintaining liquid, which is transported to molding and maintains chamber, in culturing room is made by air pressure
Maintaining liquid is pressed into molding and maintains chamber in culturing room.Two-way peristaltic pump controls the work of entire maintaining liquid circulation path, does not need
Artificial replacement maintaining liquid, maintaining liquid enter from the bottom of culturing room, culturing room are passed through after oven heats, realizes maintenance
The heating of liquid.
In the utility model, PH sensor is equipped between the two-way peristaltic pump and culturing room, the culturing room is equipped with
Tracheae, escape pipe are equipped with the second high efficiency particle air filter and carbon dioxide sensor.
In the utility model, it includes container molding and tissue pallet that the molding, which maintains chamber, and tissue pallet is located at molding
The middle part of container, container molding are respectively communicated with culturing room and two-way peristaltic pump.
In the utility model, it includes the bottom of the inlet duct on top, the pallet at middle part and lower part that the molding, which maintains chamber,
Seat, the pallet at middle part and the inlet duct on top are structure as a whole, and the sky for containing maintaining liquid is equipped among the pedestal of lower part
Chamber, the inlet duct on top and the pedestal of lower part removably connect, and inlet duct is communicated to high efficiency particle air filter, pedestal point
It is not communicated to culturing room and two-way peristaltic pump, when inlet duct is closely connect with pedestal, pallet is located at cavity.
The utility model has the advantages that during biological 3 D-printing and in biological tissue's incubation in printing later period, maintaining liquid
Environmental condition monitor and control realizations automatically by controlling microcontroller, device of the operator without repeating opening culture solution
It can get the parameters of culture solution and ambient enviroment, while regulating and controlling to keep culture solution and ring around it by micro controller unit
The stabilization in border, while contaminated possibility is also reduced, repetitive operation is higher, and experimental result is relatively reliable.
Detailed description of the invention
The utility model is done with reference to the accompanying drawings and detailed description and is further illustrated, the utility model
Above-mentioned or otherwise advantage will become apparent.
Fig. 1 is the structural schematic diagram of a kind of biological 3 D-printing environment real time monitoring and activity maintenance device；
Fig. 2 is the double-deck maintaining liquid supply chamber structural schematic diagram；
Fig. 3 is that molding maintains intracavitary portion's schematic diagram；
Fig. 4 is that molding maintains chamber schematic diagram of internal structure.
Specific embodiment
It elaborates below in conjunction with attached drawing to the utility model.
Embodiment 1:
Such as Fig. 1-Fig. 2, a kind of biological 3 D-printing environment real time monitoring provided in this embodiment and active maintenance device, packet
Include PH sensor 1, temperature sensor 2, carbon dioxide gas pressure sensor 3, oxygen flux control device 4, carbon dioxide flow control
Device 5, HEPA filter 6, two-way peristaltic pump 7, display screen 8, heating unit 9, micro controller unit 10, the closed double-deck maintenance
Liquid supply chamber 11, molding maintain chamber 12.
The double-deck maintaining liquid supply chamber 11 includes the heating room 11b positioned at the culturing room 11a on upper layer and positioned at lower layer, the training
It supports and is equipped with maintaining liquid in the 11a of room, heat transfer medium, the external carbon dioxide bottle of the culturing room and oxygen cylinder are equipped in heating room 11b,
The culturing room 11a and molding maintain 12 side of chamber to be equipped with two-way peristaltic pump 7, two-way peristaltic pump 7, culturing room 11a and molding dimension
Holding chamber 12, successively head and the tail connection forms maintaining liquid circulation path.
Temperature sensor 2 is equipped in culturing room, temperature sensor is electrically connected to microcontroller 10.
It heats and is equipped with heater 9 in the heat transfer medium of room, heater includes overheating protection circuit.
Carbon dioxide bottle is connected to CO2 flow controller 5, and oxygen cylinder is communicated to oxygen flux control device 4, titanium dioxide
Carbon flow amount controller 5 and oxygen flux control device 4 are communicated to high efficiency particle air filter 6 jointly, and high efficiency particle air filter 6 is connected to
To culturing room；The CO2 flow controller 5 and oxygen flux control device 4 are each coupled electrically to microcontroller 10.
Two-way 7 arrival end of peristaltic pump is connected to more than one media Containers, and two-way peristaltic pump outlet end is respectively communicated with training
It supports room and molding maintains chamber 12.
Culturing room 11a is sealed set.
Media Containers include maintaining liquid container, maintain to be equipped with maintaining liquid in liquid container, two-way peristaltic pump will maintain liquid container
Interior maintaining liquid is transported in culturing room, and maintaining liquid is transported to molding and maintains chamber in culturing room, and molding maintains intracavitary maintaining liquid
Flow back into two-way peristaltic pump.
PH sensor 1 is equipped between two-way peristaltic pump 7 and culturing room.Culturing room is equipped with escape pipe, and escape pipe is equipped with the
Two high efficiency particle air filter 6b and carbon dioxide sensor 3.
Carbon dioxide sensor 3 is mounted on the top of culturing room.
Temperature sensor 2 is arranged in maintaining liquid.
In the present embodiment, PH sensor 1 is used using SIN-PH4.0, the temperature sensor 2 of sinomeasure company
PT100WZP, the carbon dioxide gas pressure sensor 3 of MEACON company use the 485 type carbon dioxide 400- for building company, benevolence section
5000ppm, oxygen flux control device 4 use AIR using PK503-M, the CO2 flow controller 5 of AIR TAC company
PK503-M, HEPA filter 6 of TAC company is using Thermo Products, two-way peristaltic pump 7 using Lei Fu company
LF785, heating unit 9 use the single head heating tube of model M3.
Molding maintains the connection between the units such as chamber and each sensor, controller, heater, display screen as shown in Figure 1.
It is the place for printing that molding, which maintains chamber 12,；It is also to be used to carry out cell culture to printout after printing
Place；The activity of culture cell and maintenance cell needs stable ambient enviroment and nutrition supply, the double-deck maintaining liquid supply chamber
Stable environment and nutritional need are provided for cell.The double-deck maintaining liquid guarantees the stabilization of maintaining liquid by two-way peristaltic pump, passes through
Each sensor guarantees the stabilization of environment.
Such as Fig. 3, it is the ultra-clean cell culture workbench being arranged on print platform that molding, which maintains chamber 12, and molding maintains chamber
12 include container molding 16 and tissue pallet 17, and molding, which maintains chamber 12 whole, to be arranged in super-clean bench, and tissue is printed upon group
It knits on pallet, then tissue pallet is placed into container molding, container molding is provided with maintaining liquid, and is communicated with inlet tube
And outlet tube, it is respectively communicated with culturing room 11a and two-way peristaltic pump 7, when printing such as liver needs the tissue of blood supply, printing
It needs to maintain the blood supply of the inside after molding, therefore forms and chamber is maintained also to be connected to blood supply pipe.
Further, it is maintained on chamber 12 in molding, carbon dioxide bottle and oxygen cylinder is directly communicated to by tracheae.
The utility model for after biological 3 D-printing process and printing cell culture and development provide closing but
It can be automatically controled the environment with user's autonomous configuration.
In the utility model, high efficiency particle air filter is HEPA filter, delivery outlet and carbon dioxide content sensor peace
Mounted in the top of upper layer maintaining liquid supply chamber, temperature sensor is directly protruding into maintaining liquid.PH sensor is then mounted on maintaining liquid
With the centre for maintaining liquid medium, the present embodiment needs timing to supplement calf serum, growth for carrying out Smooth Muscle Cell
To maintain the growth of cell, the transfer tube of medium enters the media such as the factor, glucose, amino acid from heating room bottom, pass through plus
Hot cell enters maintaining liquid supply chamber.Heater 9 uses silicon rubber heater, it is indoor to be mounted on heating, while further including a mistake
Thermal protection circuit prevents temperature excessively high for closing heater in the case where temperature sensor fault.
In the utility model, it is organic that HEPA filter is mainly used for filtering out bacterium and virus in oxygen and carbon dioxide etc.
Body, therefore, it is possible to prevent and control the organisms such as bacterium and virus.
In the utility model, maintaining liquid supply chamber is also communicated with maintaining liquid output channel in addition to maintaining liquid input channel 14
15, output channel maintains chamber to provide maintaining liquid and keeps maintaining liquid recycling replacing for molding, and molding maintains chamber to be equipped with recovery approach
It maintains the maintaining liquid of chamber to recycle molding, is recycled after the monitoring of microcontroller control, guarantees to print with this
The optimum growh environment of cell culture after process and printing.
Maintaining liquid is DMEM culture medium in the present embodiment, it can provide the major part that cell has needed for the growth and breeding stage
Nutrition.Other nutrition or antibiotic are supplemented by other maintenance liquid mediums.
The two-way peristaltic pump type that the present embodiment uses manages the interior positive/negative-pressure formed, liquid is therewith for distribution type peristaltic pump
Forward and reverse movement.The peristaltic pump of the type also has communication, assignment of traffic, resorption, flow indication in addition to basic control function
And the function of correction.
Two-way peristaltic pump is opened, peristaltic pump is electrically connected with microcontroller chip, by current pH value, is controlled two-way peristaltic pump and is inhaled
Take the type and flow velocity for maintaining liquid medium.
The working principle and work of the maintenance Installation practice of this biology 3 D-printing environment real time monitoring and control system
Process is as follows:
1, before installing the maintenance device of biological 3 D-printing environment real time monitoring and control system, by all connections
Pipe and each connector, which are put into steam sterilizer sterilization bag, to carry out disinfection.All components are taken out from sterilization bag, according to
Fig. 1 connects each unit with connecting tube, fixes each sensor and heater.
2, temperature sensor is located inside culturing room, and silicon rubber heater is located at heating room.According to the indoor temperature of culture
The real time temperature that sensor monitors, the opening and closing of heater meeting automatic immediate, to keep temperature needed for maintaining liquid.
Heating interior further includes an overheating protection circuit, for closing heater in the case where temperature sensor fault.This implementation
Example, as temperature set-point controller, is used cooperatively K-type thermocouple using AD597.The setting of temperature is raw according to different cells
It is long to require to set.The circuit of the present embodiment design can use under conditions of environment temperature is -20~+260 degrees Celsius.
3, gas mixing system produces oxygen and titanium dioxide containing predetermined percentage using two gas flow controllers
Carbon mix.Gas concentration lwevel is continuously monitored using carbon dioxide sensor, utilizes the flow control of oxygen and carbon dioxide
Device effectively regulates and controls oxygen content and air pressure.The mixed gas of oxygen and carbon dioxide is passed through training after HEPA filter
Support room 11a.The cell of different plant species is to CO2The tolerance of concentration be all it is different, the concentration of general carbon dioxide is no more than
15%, otherwise there will be inhibiting effect to the growth of cell.The concentration of the carbon dioxide of the present embodiment need to be maintained at 5%.Mixing
Gas, through recycling, comes out in maintaining liquid supply chamber from venthole, into carbon dioxide content sensor after be discharged.
4, the pH value of maintaining liquid is measured by pH sensor, and pH sensor is mounted on culturing room and is situated between to dress maintaining liquid
Among the container of matter.The pH value of record is used to calibrate the input quantity of each medium.By using two-way compacted with adjustable flow velocity
Dynamic pump, so that maintaining liquid maintains to be recycled between chamber in two-way peristaltic pump, supply chamber and molding.Maintaining liquid passes through double
To peristaltic pump, by PH sensor, into maintaining liquid supply chamber, then from maintaining liquid supply chamber flow into molding maintain chamber, again from
Molding maintains chamber outflow, and circulation is supplied to two-way peristaltic pump.
5, the data that all the sensors receive, including temperature, pH value, the state of carbon dioxide control system can all be shown
Show on a display screen, and serial ports can be used and be transferred to PC.
6, after biological 3 D-printing process, the molding of Fig. 1 can directly be maintained to the connection of chamber and culture solution supply chamber
Pipe disconnects, and can also remove it from print platform.The process and principle of above-mentioned 1 to 5 step are repeated, after the completion of realizing to printing
The real-time monitoring of the cell culture environment of molded part and control.
Embodiment 2:
Such as Fig. 4, but when needing to print culture such as cartilaginous tissue, need to apply mechanical stimulation to it after shaping, to improve
The mechanical property of tissue.It is in place of the present embodiment and the difference of embodiment 1, molding maintains chamber that then Fig. 3 is changed to shown in Fig. 4
Structure, other parts are identical, the pallet 19 of inlet duct 18, middle part including top and the pedestal 20 of lower part.Top into
Device of air 18 is used to be passed through gas, and gas flows out after closed culture cavity circulation.The pallet 19 at middle part is used to place printing
Good model, maintaining liquid and gas exchanges needed for the pedestal 20 of lower part is used to supply cell growth and breeding.The pallet at middle part
19 are integrated with the inlet duct on top, can be embedded in or leave lower part by stretching and compressing top.Need place or
When taking out model, need to close maintaining liquid and air intake valve.The pedestal middle cavity of lower part be used for be equipped with maintaining liquid, top into
Device of air and the pedestal of lower part being capable of pin 21 and the closely connections of hole 22.When inlet duct is closely connect with pedestal, 19, pallet
At cavity 23.
When the present embodiment is in use, printed cartilaginous tissue is placed on pallet, by the inlet duct on top under
The pedestal in portion is closely connected by pin hole, is then ventilated and is passed through maintaining liquid, pressurizes and promote mechanical stimulation, to improve
The mechanical property of tissue.
The utility model provides a kind of biological 3 D-printing environment real time monitoring and active maintenance device, and specific implementation should
There are many method and approach of technical solution, and the above is only the preferred embodiment of the utility model, it is noted that for this
For the those of ordinary skill of technical field, without departing from the principle of this utility model, several improvement can also be made
And retouching, these improvements and modifications also should be regarded as the protection scope of the utility model.Each composition portion being not known in the present embodiment
The available prior art is divided to be realized.
Claims (10)
1. a kind of biology 3 D-printing environment real time monitoring maintains device with activity, which is characterized in that supplied including the double-deck maintaining liquid
Chamber (12) are maintained to room (11) and the molding being placed in super-clean bench, the bilayer maintaining liquid supply chamber (11) includes being located at upper layer
Culturing room (11a) and heating room (11b) positioned at lower layer, be equipped with maintaining liquid in the culturing room (11a), heat room (11b)
It is interior to be equipped with heat transfer medium, the external carbon dioxide bottle of the culturing room and oxygen cylinder, the culturing room (11a) and molding maintenance chamber
(12) side is equipped with two-way peristaltic pump (7), and two-way peristaltic pump (7), culturing room (11a) and molding maintain chamber (12) successively to connect from beginning to end
It is logical to form maintaining liquid circulation path.
2. a kind of biological 3 D-printing environment real time monitoring according to claim 1 maintains device with activity, feature exists
In equipped with temperature sensor (2) in the culturing room, temperature sensor is electrically connected to microcontroller (10).
3. a kind of biological 3 D-printing environment real time monitoring according to claim 2 maintains device with activity, feature exists
In equipped with heater (9) in the heat transfer medium of the heating room, heater includes overheating protection circuit.
4. a kind of biological 3 D-printing environment real time monitoring according to claim 3 maintains device with activity, feature exists
In the carbon dioxide bottle is connected to CO2 flow controller (5), and oxygen cylinder is communicated to oxygen flux control device (4), dioxy
Change carbon flow amount controller (5) and oxygen flux control device (4) is communicated to high efficiency particle air filter (6a), high-efficiency air filtering jointly
Device (6a) is communicated to culturing room；The CO2 flow controller (5) and oxygen flux control device (4) are each coupled electrically to micro-control
Device (10) processed.
5. a kind of biological 3 D-printing environment real time monitoring according to claim 4 maintains device with activity, feature exists
In two-way peristaltic pump (7) the arrival end connection maintains liquid container and media Containers, two-way peristaltic pump outlet end to be respectively communicated with
Chamber (12) are maintained to culturing room and molding.
6. a kind of biological 3 D-printing environment real time monitoring according to claim 5 maintains device with activity, feature exists
In the culturing room (11a) is sealed set.
7. a kind of biological 3 D-printing environment real time monitoring according to claim 6 maintains device with activity, feature exists
In the media Containers include maintaining liquid container, maintain to be equipped with maintaining liquid in liquid container, two-way peristaltic pump will maintain in liquid container
Maintaining liquid be transported in culturing room, maintaining liquid is transported to molding and maintains chamber in culturing room, and molding maintains intracavitary maintaining liquid to return
Flow to two-way peristaltic pump.
8. a kind of biological 3 D-printing environment real time monitoring according to claim 7 maintains device with activity, feature exists
In being equipped with PH sensor (1) between the two-way peristaltic pump (7) and culturing room, the culturing room is equipped with escape pipe, on escape pipe
Equipped with the second high efficiency particle air filter (6b) and carbon dioxide sensor (3).
9. a kind of biological 3 D-printing environment real time monitoring according to claim 8 maintains device with activity, feature exists
In it includes container molding (16) and tissue pallet (17) that the molding, which maintains chamber (12), and tissue pallet (17) is located at molding and holds
The middle part of device (16), container molding (16) are respectively communicated with culturing room (11a) and two-way peristaltic pump (7).
10. a kind of biological 3 D-printing environment real time monitoring according to claim 8 maintains device with activity, feature exists
In, it includes the pedestal (20) of the inlet duct (18) on top, the pallet (19) at middle part and lower part that the molding, which maintains chamber (12),
The pallet (19) at middle part and the inlet duct on top are structure as a whole, and the sky for containing maintaining liquid is equipped among the pedestal of lower part
Chamber (23), the inlet duct (18) on top are removably connect with the pedestal (20) of lower part, and inlet duct (18) is communicated to efficiently
Air filter (6a), pedestal (20) is respectively communicated with culturing room (11a) and two-way peristaltic pump (7), when inlet duct and pedestal
When close connection, pallet (19) is located at cavity (23).
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| CN201821269314.1U CN208917207U (en) | 2018-08-08 | 2018-08-08 | A kind of biology 3 D-printing environment real time monitoring and active maintenance device |
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