CN208649292U - A kind of egg mother cell fluorescent staining experiment device for being protected from light cleaning cell - Google Patents
A kind of egg mother cell fluorescent staining experiment device for being protected from light cleaning cell Download PDFInfo
- Publication number
- CN208649292U CN208649292U CN201821188924.9U CN201821188924U CN208649292U CN 208649292 U CN208649292 U CN 208649292U CN 201821188924 U CN201821188924 U CN 201821188924U CN 208649292 U CN208649292 U CN 208649292U
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- protected
- light
- egg mother
- mother cell
- cylinder
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Abstract
A kind of egg mother cell fluorescent staining experiment device for being protected from light cleaning cell, is related to field of biotechnology.When the utility model solves existing progress egg mother cell fluorescent staining experiment, the problem of process of egg mother cell easily leads to fluorescent quenching is cleaned.It include be protected from light lid, be vertically arranged be protected from light cylinder, be packed in the strainer for being protected from light cylinder body bottom and be coaxially fixed in the support for being protected from light cylinder bottom end, the sieve pore of strainer is less than egg mother cell, it is protected from light lid and is located at the top for being protected from light cylinder with closing lid, in a ring, and the internal diameter of holder top is identical as the internal diameter for being protected from light cylinder for the cross section of support.
Description
Technical field
The utility model relates to a kind of egg mother cell fluorescent staining experiment devices for being protected from light cleaning cell, are related to biological skill
Art field.
Background technique
Egg mother cell is frequently necessary to be washed repeatedly with different solution when carrying out fluorescent staining experiment, these are different molten
Liquid is located in different containers, and when by egg mother cell by being transferred in another solution in a solution, need section
Grind personnel and put egg mother cell into another solution again by being sucked out in a solution with mouth suction pipe, draw ovum process must
Microscope must be used, using the position of microscopical strong light identification egg mother cell accurately to draw, but is easy to lead during this
Being quenched for fluorescence is caused, to influence the accuracy of experimental result.A variety of different solution for cleaning are placed on more simultaneously
In a container, area occupied is big, and cleaning efficiency is low.
Utility model content
When the utility model is to solve existing progress egg mother cell fluorescent staining experiment, the mistake of egg mother cell is cleaned
Journey easily leads to the problem of fluorescent quenching, and then provides the egg mother cell fluorescent staining experiment device for being protected from light cleaning cell.
Used technical solution is the utility model to solve above-mentioned technical problem:
A kind of egg mother cell fluorescent staining experiment device for being protected from light cleaning cell, it includes being protected from light lid, being vertically arranged
It is protected from light cylinder, be packed in the strainer for being protected from light cylinder body bottom and is coaxially fixed in the support for being protected from light cylinder bottom end, the sieve pore of strainer
Less than egg mother cell, lid being protected from light with closing lid and is located at the top for being protected from light cylinder, in a ring, and holder top is interior for the cross section of support
Diameter is identical as the internal diameter for being protected from light cylinder.
Further, a kind of egg mother cell fluorescent staining experiment with the device for being protected from light cleaning cell further includes pedestal and several
Absorbent filter offers the groove of opening upwards on pedestal, and several absorbent filters are stacked in groove, and support rides upon bottom
The top of seat, and face is arranged up and down for strainer and absorbent filter.
Further, absorbent filter is circular filter paper, and the diameter of absorbent filter is identical as the internal diameter for being protected from light cylinder.
Further, the outer diameter of holder top is less than the outer diameter of bottom end.
Further, the altitude range for being protected from light cylinder is 0.8cm~1.2cm.
The utility model has the effect that compared with prior art
The process of cleaning egg mother cell is to be protected from light state, and by the way of, replacement solution motionless using ovum, do not need
It sees whether to be drawn to egg mother cell in microscope, therefore also avoids the illumination under microscope and cause egg mother cell fluorescence
Be quenched, can be effectively protected from light with existing method ratio, reduce interference (being quenched) to fluorescent reagent used in experiment, cleaning efficiency is more
The accuracy of height, experimental result is more preferable, and the application structure is simple, and area occupied is smaller.
Detailed description of the invention
Fig. 1 is the main cross-sectional schematic diagram of the utility model；
Fig. 2 is the schematic top plan view (being protected from light lid to be not shown) of Fig. 1；
Fig. 3 is the cooperation schematic diagram that cylinder and centrifuge tube are protected from light when collecting egg mother cell.
Specific embodiment
Specific embodiment 1: illustrate present embodiment in conjunction with Fig. 1~3, a kind of egg mother cell fluorescent staining experiment is with keeping away
Light clean cell device, it include be protected from light lid 1, be vertically arranged be protected from light cylinder 2, be packed in the strainer 3 for being protected from light 2 bottom of cylinder
And it is coaxially fixed in the support 4 for being protected from light 2 bottom end of cylinder, the sieve pore of strainer 3 is less than egg mother cell, is protected from light lid 1 and is located at closing lid
It is protected from light the top of cylinder 2, in a ring, and the internal diameter on 4 top of support is identical as the internal diameter for being protected from light cylinder 2 for the cross section of support 4.It keeps away
Light lid 1, the washing container for being protected from light cylinder 2 and the composition egg mother cell of strainer 3, egg mother cell and cleaning are both placed in solution and are protected from light
On strainer 3 in cylinder 2, because of the tension force effect of solution itself, when cleaning egg mother cell solution can't permeable screens 3, more
Renew and be sucked out with the solution that absorbent filter 6 is adjacent to strainer 3 will be protected from light in cylinder 2 before solution, remove absorbent filter 6, to being protected from light
The new solution of injection in cylinder 2, circulate operation, is completed until egg mother cell cleans according to this.The whole process of cleaning is to be protected from light state,
And by the way of, replacement solution motionless using ovum, do not need to see whether to be drawn to egg mother cell in microscope, therefore
It avoids the illumination under microscope and causes being quenched for egg mother cell fluorescence, cleaning efficiency is higher, and accuracy is more preferable, and this Shen
Please structure it is simple, area occupied is smaller.The top of support 4 is close to the one end for being protected from light cylinder 2, and the bottom end of support 4 is separate keeps away
One end of light cylinder 2.
The sieve pore density of strainer guarantees that egg mother cell is not passed through but does not interfere solution to pass through.
Egg mother cell is collected in brown centrifuge tube 7 after fluorescent staining, carries out simple centrifugally operated.It keeps away
The outer diameter of light cylinder 2 is identical as 7 internal diameter of centrifuge tube.The outer diameter of 4 bottom end of support is identical as the outer diameter of centrifuge tube 7.
A kind of egg mother cell fluorescent staining experiment further includes pedestal 5 and several absorbent filters with the device for being protected from light cleaning cell
6, the groove 51 of opening upwards is offered on pedestal 5, several absorbent filters 6 are stacked in groove 51, and support 4 rides upon bottom
The top of seat 5, and strainer 3 and about 6 face of absorbent filter are arranged.When needing replacing the solution being protected from light in cylinder 2, will have
Support 4 and the cylinder 2 that is protected from light for being protected from light lid 1 ride upon on pedestal 5, and guarantee that the absorbent filter 6 of 3 lower section of strainer and strainer 3 are tight
Contiguity touching will be with support 4 and be protected from light lid 1 be protected from light cylinder after the solution being protected from light in cylinder 2 is absorbed by absorbent filter 6
Body 2 is removed from pedestal 5, and opening is protected from light lid 1 and injects new solution, according to this circulate operation, is completed until egg mother cell cleans.It is logical
Crossing the application can make working environment cleaner and tidier, and be not take up extra space.
The outer diameter on 4 top of support is less than the outer diameter of bottom end.Make to be protected from light cylinder 2 on being placed into work top or pedestal 5
When it is more firm.
Absorbent filter 6 is circular filter paper, and the diameter of absorbent filter 6 is identical as the internal diameter for being protected from light cylinder 2.Guarantee water suction filter
Paper 6 and strainer 3 are in close contact, and guarantee that strainer 3 is capable of contacting with absorbent filter 6 for maximum area, so as to be protected from light in cylinder 2
Solution be sucked out completely.
The altitude range for being protected from light cylinder 2 is 0.8cm~1.2cm, preferably 1.0cm.
Claims (5)
1. a kind of egg mother cell fluorescent staining experiment device for being protected from light cleaning cell, it is characterised in that: it includes being protected from light lid
(1), what is be vertically arranged is protected from light cylinder (2), is packed in the strainer (3) for being protected from light cylinder (2) bottom and is coaxially fixed in and is protected from light cylinder
The support (4) of body (2) bottom end, the sieve pore of strainer (3) are less than egg mother cell, are protected from light lid (1) and match closing lid and be located at and are protected from light cylinder (2)
Top, in a ring, and the internal diameter on support (4) top is identical as the internal diameter for being protected from light cylinder (2) for the cross section of support (4).
2. a kind of egg mother cell fluorescent staining experiment device for being protected from light cleaning cell according to claim 1, feature
Be: it further includes pedestal (5) and several absorbent filters (6), and the groove (51) of opening upwards is offered on pedestal (5), several
Absorbent filter (6) is stacked in groove (51), and support (4) rides upon the top of pedestal (5), and strainer (3) and water suction are filtered
Face is arranged paper (6) up and down.
3. a kind of egg mother cell fluorescent staining experiment device for being protected from light cleaning cell according to claim 1 or 2, special
Sign is: absorbent filter (6) is circular filter paper, and the diameter of absorbent filter (6) is identical as the internal diameter for being protected from light cylinder (2).
4. a kind of egg mother cell fluorescent staining experiment device for being protected from light cleaning cell according to claim 3, feature
Be: the outer diameter on support (4) top is less than the outer diameter of bottom end.
5. a kind of egg mother cell fluorescent staining experiment device for being protected from light cleaning cell according to claim 3, feature
Be: the altitude range for being protected from light cylinder (2) is 0.8cm~1.2cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821188924.9U CN208649292U (en) | 2018-07-25 | 2018-07-25 | A kind of egg mother cell fluorescent staining experiment device for being protected from light cleaning cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821188924.9U CN208649292U (en) | 2018-07-25 | 2018-07-25 | A kind of egg mother cell fluorescent staining experiment device for being protected from light cleaning cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208649292U true CN208649292U (en) | 2019-03-26 |
Family
ID=65786312
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821188924.9U Expired - Fee Related CN208649292U (en) | 2018-07-25 | 2018-07-25 | A kind of egg mother cell fluorescent staining experiment device for being protected from light cleaning cell |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208649292U (en) |
-
2018
- 2018-07-25 CN CN201821188924.9U patent/CN208649292U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190326 Termination date: 20190725 |