CN208636327U - Small size automatic enzyme exempts from constant temperature analyzer - Google Patents
Small size automatic enzyme exempts from constant temperature analyzer Download PDFInfo
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- CN208636327U CN208636327U CN201820795226.9U CN201820795226U CN208636327U CN 208636327 U CN208636327 U CN 208636327U CN 201820795226 U CN201820795226 U CN 201820795226U CN 208636327 U CN208636327 U CN 208636327U
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- suction nozzle
- suction
- heating
- constant temperature
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Abstract
The utility model discloses a kind of small size automatic enzymes to exempt from constant temperature analyzer, suction mechanism, pipetting device, transverse-moving mechanism, six part of tray drive mechanism, constant temperature system and photoelectric detection mechanism.Suction mechanism mainly completes the suction of liquid and taking for suction nozzle moves back function；Pipetting device, transverse-moving mechanism and tray drive mechanism are to realize for the reagent for having been drawn into suction nozzle to be moved to and postpone carry out drain to reaction warehouse storage space, pipetting device is responsible for the movement of entire suction mechanism or more, transverse-moving mechanism is responsible for the movement of entire suction mechanism or so, pallet driving changes corresponding storage position, to realize that suction nozzle protrudes into and stretch out reaction warehouse；Constant temperature system in order to realize to sample storehouse heating and suction nozzle heating and heat preservation；Detection part is in order to realize the detection to reagent reaction substrate.
Description
Technical field
It is efficient and convenient using one kind of enzyme immune system design based on STM32 microprocessor that the utility model relates to a kind of
Biochemistry detection equipment can especially be automatically performed and take desorption head.
Background technique
Most domestic product is not really full-automatic at present, it usually needs is located in advance before experiment to reagent
Then reason completes subsequent analysis using analyzer and works.External a small number of products can complete a whole set of enzyme and exempt from analysis process, still
Its price is costly.
Summary of the invention
In order to overcome different reagents experimental situation, reagent quantitative, reaction sequence difference and cause detection device can not
Realize that automatically the utility model provides a kind of detection device, which can not only realize that full automatic enzyme exempts to analyze,
And it may be implemented to take desorption head automatically.
The utility model tester movement mechanism is divided into 5 modules, respectively suction mechanism, pipetting device and traversing
Mechanism, tray drive mechanism, constant temperature system, photoelectric detection mechanism.
Suction mechanism mainly completes the suction of liquid and taking for suction nozzle moves back function.Wherein power plant module be sampling system from
The realization of dynamicization function, due to this system have both take desorption greatest service can stroke in the vertical direction it is larger, the straight line of big stroke
Motor be easy to cause waste spatially, so realizing the movement of vertical direction in such a way that stepper motor drives lead screw pair.
Wherein substrate realizes the moving left and right to realize multichannel of liquor-transferring system as expanding plate and can increase horizontal slide rail on substrate
Detection.Optoelectronic switch is housed on substrate, the detectable contact stretched out from lifting plate is to detect the stretching of sample introduction needle piston rod
Length, the working condition and initialized location run with this calibration system.It is prevented in substrate the upper side and lower side equipped with limit switch
The fortuitous events such as motor overshoot or control failure.Biochemical Analyzer needs to carry out multiple hybrid reaction in the detection process, in order to
The extraction raffinate pollution reaction for avoiding residual cavity inner wall, carries out suction liquid relief often through pipetting tip and replaces after the completion of reaction
Pipetting tip guarantees the reliability of lower secondary response, need to take the structure of suction nozzle and desorption head in the installation of sample injector head thus, and use O
The sealing of type sealing ring.Suction nozzle mounting post and suction nozzle are installed by static friction, and desorption head top cap can trigger limit when suction nozzle is plugged
Bit switch feedback switch amount shows that suction nozzle has taken to single-chip microcontroller.Pass through control Step Motor movable plate when desorption head
Suction nozzle is decorporated to push desorption head top cap.Compressed spring is laid between desorption head top cap and movable plate prevents desorption head
Top cover contacts stuck with movable plate inner wall.
Pipetting device be in order to realize for the reagent for having been drawn into suction nozzle to be moved to and postpone carry out drain to reaction warehouse storage space,
The mobile mutual cooperation with pallet is needed among these, and pallet driving changes corresponding storage position, and pipetting device is then responsible for whole
Movement above and below a suction mechanism, to realize that suction nozzle protrudes into and stretch out reaction warehouse.The suspension of suction mechanism is installed on carrying backboard
Line slide rail on, drive lead screw pair to realize the oscilaltion of entire suction mechanism by stepper motor, the support frame in two sides
Corresponding position is equipped with slot type optoelectronic switch, when pipetting device drives suction mechanism to move up and down to designated position, carries backboard
On optoelectronic switch baffle will shelter from the infrared detection line of slot type optoelectronic switch, sensor issues response signal to master at this time
Unit is controlled, to stop whole movement, then begins to carry out suction movement.
Transverse-moving mechanism is mainly that the angular displacement of rotation is changed into straight line position by the screw rod stepper motor passed through on carrying backboard
It moves, realizes the transverse shifting of suction mechanism on the slide rail, equipped with baffle to trigger a left side on the nut of lead screw stepper motor
The slot type optoelectronic switch of right two extreme positions, it is two different channels on reagent tray that the two positions are corresponding,
For channel switch, the test experience in a usual channel be it is identical, can thus under same primary experimental implementation
Complete two groups of different classes of test experiences.
Tray drive mechanism is different from the driving method of pipetting device and transverse-moving mechanism, becomes rack-and-pinion by lead screw pair
Pair reduces design space, and running track is equally line slide rail, and rack gear is mounted on reagent tray side, and gear pair is mounted on
On stepper motor, drive rack-and-pinion that angular displacement is converted into straight-line displacement by stepper motor.Equally in crucial storage position
Slot type optoelectronic switch is needed to be positioned.
Constant temperature system is always the key points and difficulties that automatic enzyme exempts from analytical equipment, because of the work of marker enzyme and sample to be detected
Property experimental result exempted from enzyme influence very big, therefore, for the activity for keeping marker enzyme and sample to be detected, the room temperature of instrument answered
It is maintained at 37 ± 0.2 DEG C, and carries out 100 DEG C again in the pretreatment stage of sample and be evenly heated, for this same environment
The equipment that lower heating temperature requires needs to carry out control from many aspects such as mechanism and control to reach the heating of regional temperature
It is required that.Wherein at 100 DEG C of sample storehouse area requirement, reagent suction nozzle region needs to carry out quick 37 DEG C due to the reason of coating medium
Heating and thermal insulation.
There are many modes in constant temperature system, be broadly divided into: air dry bath, water bath with thermostatic control, solid type dry bath, Peltier.It is permanent
Although tepidarium is in control in temperature uniformity, it is not suitable for heating inside equipment.So for 100 DEG C of heating
Heating with suction nozzle takes solid type dry bath and Peltier heating respectively, and wherein heat affected zone uses the good aluminium of heat-conducting effect
Alloy is processed, ensure that temperature be heated evenly and thermal insulation property.Because under a heating system, with one in mechanism
A good connection frame of heat dissipation is coupled, and avoids influencing suction nozzle region in 100 DEG C of heating process.Wherein sample is located in advance
The heating of reason uses heating rod and is heated, and the heat transfer of heating rod to sample is stored in a warehouse by rising sample heating zone
It goes, realizes heating process.The heating method of suction nozzle is similar, but since temperature requirements are not high and the factors such as heating uniformity, uses
The heating method of flexible heater piece.For the variation of real-time monitoring and control temperature, the critical positions in heating zone are provided with
Temperature sensor, to feed back the inner case of heating zone.
Photoelectric detection mechanism is the core of biochemical analyzer, and to the execution machine that reaction substrate is detected
Structure.The photoelectric detection system of traditional analyzer is developed on the basis of spectrophotometer, small-sized in order to reach instrument
The purpose of change, the utility model is based on spectroanalysis instrument and devises Novel light path detection system, by light source, light path system and spectrum
Analyzer composition, central principle are that composite light source projects holographic concave grating and is divided, by detector to be divided into
Row detection.The advantages of detector is optocoupler components, the element is each pixel very little, and the center between each pixel away from
It is very small, therefore detection accuracy, sensitivity are very high.By micro spectral analyzer, the conversion of optical signal and electric signal is realized.
This detection system is light splitting afterwards technology, is to be put into grating beam splitting process after light source enters reaction tank to carry out, therefore, pole
The complexity of big reducing mechanism realizes that detection process with the separation of conversion zone, avoids light sensitive component from being constantly in moist ring
Border and damage, be conducive in this way improve instrument precision and the service life.
ELISA Auto Analyze System is the shade according to reaction substrate, to judge extent.By lambert Bill
For law it is found that under single light source irradiation, the absorbance of solution is directly proportional to the product of solution concentration and solution layer thickness, passes through
The concentration for calculating available substance, its calculation formula is:
Wherein, A is absorbance, I0For incident intensity, I is transmitted intensity, and ε is molar absorption coefficient, and b is that solution is dense
Degree, C is sample path length.It is beaten by the incident light that light source emits some strength in transparent substrate reactions storehouse, incident light is the bottom of by
Transmitted light is formed after object solution, transmitted light is shining into optical detection device and is AD converted to obtain digital signal, then the letter that will be obtained
It number is sent in processor and to carry out rational judgment, obtain final result.
Photodetector unit can only once detect the reaction substrate situation of a storage, use translation to save space
Photodetector unit is moved to specified storage position by mechanism, then carries out illumination experiment.Wherein entire light checking device is mounted on
On the perforative optical axis guide rail of ball screw assembly, both ends slot type optoelectronic switch setting limit position, ball-screw has stepper motor
Belt pulley is driven to provide power source.Rotational angle by calculating practical stepper motor realizes the quantitative movement of Photoelectric Detection.
The utility model has the beneficial effects that can be completed while being automatically finished multiple groups EIA enzyme immunoassay
Automatically desorption head is taken.
Detailed description of the invention
The present invention will be further described with reference to the accompanying drawings and examples.
Fig. 1 sampling system suction structural schematic diagram
Fig. 2 sample injector head mechanism schematic diagram
Fig. 3 sampling system pipetting device schematic diagram
Fig. 4 sampling system transverse-moving mechanism schematic diagram
Fig. 5 pallet driving structure schematic diagram
Fig. 6 heating structure schematic diagram
Fig. 7 light path schematic diagram
Fig. 8 photoelectric detection mechanism
Wherein 1. sample injector cavity, 2.v slot pressing plate, 3. plunger rods, 4. lifting fixed plates, 5. limit switches, 6. is synchronous
It takes turns, 7. synchronous belts, 8. suction stepper motors, 9. linear bearings, 10. optical axises, 11. suction mechanism bases, on 12. sealing plates, 13.
Under sealing plate, 14. movable plates, 15. suction nozzle mounting posts, 16. desorption skulls, 17. suction nozzles, 18. desorption head inner covers, 19. limit open
It closes, 20.O type sealing ring, 21. sample injector attachment bases, 22. sealing rings, 23. liquid relief stepper motors, 24. synchronizing wheels, 25. tensionings
Wheel, 26. lamina tectis, 27. side skeletons, 28. optoelectronic switches, 29. optoelectronic switch baffles, 30. ball-screw nuts, 31. ball wires
Thick stick, 32. traversing screw rod stepper motors, 33. carrying backboards, 34. linear guides, 35. position optoelectronic switches, 36. position photoelectricity are opened
It closes, 37. sample storehouses, 38. reagent strips, 39. reagent trays, 40. secondary gears, 41. stepper motor fixed frames, the driving of 42. pallets
Stepper motor, 43. side skeletons, 44. guide rail connection frames, 45. baffles, 46. linear guides, 47. slot type optoelectronic switches, 48. samples
Storehouse heating zone, 49. synchronous belts, 50. heating rods, 51. heating connecting plates, 52. ball-screw nuts, 53. heating zones, 54. is flexible
Heating sheet, 55. synchronizing wheels, 56. screw pair fixed frames.57. light source, 58. incident lights, 59. substrate reactions storehouses, 60. transmitted lights,
61. light-sensitive element.62. light examines circuit mounting plate, 63. light examine mounting rack, and 64. initial optoelectronic switches, 65. terminate optoelectronic switch,
66. pedestal, 67. mobile platforms, 68. optical axis guide rails, 69. ball-screws.
Specific embodiment
Small size automatic Enzyme Immunoassay Analyzer be divided into suction mechanism such as Fig. 1, pipetting device such as Fig. 3, transverse-moving mechanism such as Fig. 4,
Tray drive mechanism such as Fig. 5, constant temperature system such as Fig. 6 and photoelectric detection mechanism such as Fig. 8.
V slot pressing plate 2 is fixed on the base 11 by sample injector cavity 1 in suction mechanism, and suction stepper motor 8 passes through synchronization
Belt wheel 6 and synchronous belt 7 and screw pair drive lifting fixed plate 4, and the plunger rod 3 being fixed thereon is made to move up and down, and realize
Suction function.Suction nozzle 17 is connect with suction nozzle mounting post 15 by static friction, and desorption skull 16 can be triggered when suction nozzle 17 is plugged
19 feedback switch amount of limit switch shows that suction nozzle 17 has taken to single-chip microcontroller.Pass through control Step Motor when desorption head
Movable plate 14 decorporates suction nozzle 17 to push desorption head inner cover 18.
Liquid relief stepper motor 23 and ball-screw 31 are fixed in top cover version 26 and are supported by side skeleton 27 in pipetting device,
The suspension of suction mechanism is installed on the line slide rail 34 of carrying backboard 33, screw pair is driven by liquid relief stepper motor 23
Realize the oscilaltion of entire suction mechanism.
Transverse-moving mechanism is to realize entire discharging machine with moving base 11 by traversing lead screw stepper motor 32 on carrying backboard 33
Structure carries out transverse shifting in linear guide 34.
Pallet driving stepper motor 42 by secondary gear 40 and is fixed on reagent tray 39 in tray drive mechanism
Rack gear realizes that reagent tray 39 drives reagent strip 38 to move along linear guide 46.
Constant temperature system carries out 100 DEG C of heating to sample storehouse heating zone 48 by heating rod 50, right by flexible heater piece 54
Suction nozzle carries out 37 DEG C of heating and thermal insulations, and the good heating connecting plate 51 of two parts heat dissipation connects.
Light source 57 issues incident light 58 in photoelectric detection mechanism, transmitted light is formed behind substrate reactions storehouse 59, thoroughly
It penetrates light 60 and is shining into light-sensitive element 61.Photoelectric detection mechanism can only once detect the reaction substrate situation of a storage, utilize lead screw
Pair of nut makes entire photoelectric detection mechanism be moved to the position for needing to detect on optical axis guide rail 68.
Claims (2)
1. small size automatic enzyme exempts from constant temperature analyzer, including suction mechanism, pipetting device, transverse-moving mechanism, tray drive mechanism, perseverance
Warm system and photoelectric detection mechanism, it is characterised in that: suction nozzle (17) is connect with suction nozzle mounting post (15) by static friction, desorption head
Lid (16) can trigger limit switch (19) feedback switch amount when suction nozzle (17) is plugged to single-chip microcontroller, show suction nozzle (17)
It takes, is moved back suction nozzle (17) to push desorption head inner cover (18) by control Step Motor movable plate (14) when desorption head
It goes, completes to take desorption head automatically.
2. small size automatic enzyme according to claim 1 exempts from constant temperature analyzer, which is characterized in that right by heating rod (50)
Sample storehouse heating zone (48) carries out 100 DEG C of heating, carries out 37 DEG C of heating and thermal insulations, two parts to suction nozzle by flexible heater piece (54)
With good heating connecting plate (51) connection of radiating.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820795226.9U CN208636327U (en) | 2018-05-20 | 2018-05-20 | Small size automatic enzyme exempts from constant temperature analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820795226.9U CN208636327U (en) | 2018-05-20 | 2018-05-20 | Small size automatic enzyme exempts from constant temperature analyzer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208636327U true CN208636327U (en) | 2019-03-22 |
Family
ID=65732763
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820795226.9U Expired - Fee Related CN208636327U (en) | 2018-05-20 | 2018-05-20 | Small size automatic enzyme exempts from constant temperature analyzer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208636327U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110068695A (en) * | 2019-04-19 | 2019-07-30 | 成都恩普生医疗科技有限公司 | A kind of handheld portable urine automabic analyzer device device |
| CN112730859A (en) * | 2019-10-28 | 2021-04-30 | 东方伊诺(苏州)医疗科技有限公司 | Automatic enzyme-linked immunoassay analyzer |
-
2018
- 2018-05-20 CN CN201820795226.9U patent/CN208636327U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110068695A (en) * | 2019-04-19 | 2019-07-30 | 成都恩普生医疗科技有限公司 | A kind of handheld portable urine automabic analyzer device device |
| CN110068695B (en) * | 2019-04-19 | 2024-02-06 | 成都恩普生医疗科技有限公司 | Handheld portable automatic urine analysis instrument device |
| CN112730859A (en) * | 2019-10-28 | 2021-04-30 | 东方伊诺(苏州)医疗科技有限公司 | Automatic enzyme-linked immunoassay analyzer |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190322 Termination date: 20200520 |