CN208594224U

CN208594224U - A kind of device extracting stevioside from STEVIA REBAUDIANA

Info

Publication number: CN208594224U
Application number: CN201821075672.9U
Authority: CN
Inventors: 于发东
Original assignee: SHANDONG DUANXINTANG DAYU PHARMACEUTICAL CO Ltd
Current assignee: SHANDONG DUANXINTANG DAYU PHARMACEUTICAL CO Ltd
Priority date: 2018-07-09
Filing date: 2018-07-09
Publication date: 2019-03-12
Anticipated expiration: 2028-07-09

Abstract

The utility model discloses a kind of from STEVIA REBAUDIANA extracts the device of stevioside, including feed pipe, the side of the feed pipe is equipped with the first feeding device, first feeding device is equipped with crush box, and the crush box is equipped with grinding device, and the lower end side of the crush box, which is run through, is equipped with the second conveying pipeline, second conveying pipeline is equipped with filter device, the filter device is equipped with shell, and mixing arrangement is equipped in the shell, and the side of the mixing arrangement is equipped with primary heater；Using the above structure, it realizes and STEVIA REBAUDIANA is effectively cleaned, reduce the impurity residual in STEVIA REBAUDIANA, and STEVIA REBAUDIANA can be subjected to efficient pulverizing, can have and efficiently be screened, convenient for preferably participating in late phase reaction, improve the quality of reaction, recovery rate is improved, can effectively guarantee the activity of stevioside, improves product quality.

Description

A kind of device extracting stevioside from STEVIA REBAUDIANA

Technical field

A kind of extracting the present invention relates to steviol glycoside extractive technique field more particularly to from STEVIA REBAUDIANA stevioside Device.

Background technique

STEVIA REBAUDIANA is the evergreen dwarf shrub originated between South America Paraguay plateau south latitude 25-26 degree, each by the world Ground is introduced a fine variety, and is a kind of tetracylic diterpene derivatives, mainly by stevioside, sweet tea containing a kind of stevioside substance in blade 8 kinds of glucosides compositions such as chrysanthemum glycosides A, stevioside B and stevioside C, sugariness is about 300 times of sucrose.Studies have shown that stevia Glycosides have the characteristics that high sugariness, it is low in calories, without obvious toxic-side effects, it can inhibit hyperglycemia and hypertension, and have anti-inflammatory, anti-swollen Tumor, antidiarrheal diuresis and the immunoregulatory effect of assistance.Currently, South America, Japan and China are using stevioside as one kind Natural sweetener is widely used in food and beverage.

About extracting, stevioside report is more, and the patent of CN105801639A discloses a kind of sweetleaf in the prior art The extracting method of steviol glycoside in chrysanthemum, by only need to then be eluted leaching liquor loading to splitter and retain target stream Liquid out is concentrated and dried later, and step is less, and integrated artistic is simpler, is conducive to apply, still, inventor's research It was found that this method not can be carried out effective crushing, so that recovery rate is lower, increase cost.

The patent of CN106632539A discloses a kind of extracting method of stevioside, is returned by using ultrasonic wave added heat Stream extracts, and carries out rotary evaporation, and cost is relatively low so as to making, and extracts that gained Glycosides Contents are high, and extraction time is short, sugar Glycosides extraction comparison is thorough, and still, inventor, which studies, finds that operating condition is harsher, and STEVIA REBAUDIANA can be destroyed by needing to reach higher temperature The activity of glucosides, influences end product quality.

The patent of CN105294790A discloses a kind of method that high-purity stevioside is extracted from STEVIA REBAUDIANA, passes through The method that enzyme immersion, heating extraction, flocculation purification combine improves stevioside leaching content, while making in STEVIA REBAUDIANA Impurity the amount of dissolution minimize, facilitate subsequent purification, at low cost, strong operability, be suitble to plant produced, still, inventor research It was found that being located at STEVIA REBAUDIANA carries out thorough cleaning, it is easy that the remaining impurity of sweetleaf is made to enter production procedure, is not easy to clear up, causes to produce Contain impurity in product, influences product quality.

To sum up, the prior art is when extracting stevioside, it may appear that STEVIA REBAUDIANA utilization rate is low, causes recovery rate It is low, cause cost of material to increase；Stevioside activity is destroyed, product quality is influenced；It cannot be cleared up, be easy to appear very well The problems such as impurity remains, and influences product quality, how while effectively improving recovery rate, guarantees that product quality is this field skill The key points and difficulties problem of art personnel research.

The device and its extracting method of stevioside are extracted for this purpose, we have proposed a kind of from STEVIA REBAUDIANA, to solve The above problem.

Summary of the invention

The purpose of the present invention is to solve disadvantages existing in the prior art, and the one kind proposed is extracted from STEVIA REBAUDIANA The device and its extracting method of stevioside.

To achieve the goals above, present invention employs following technical solutions:

A kind of device extracting stevioside from STEVIA REBAUDIANA, including feed pipe, the side of the feed pipe are equipped with the One feeding device, first feeding device are equipped with crush box, and the crush box is equipped with grinding device, the crush box Lower end side, which is run through, is equipped with the second conveying pipeline, and second conveying pipeline is equipped with filter device, and the filter device is equipped with shell Body, is equipped with mixing arrangement in the shell, and the side of the mixing arrangement is equipped with primary heater, and the one of the primary heater Side is connected with ultrasonic generator, and the side of the ultrasonic generator is connected with the first holding vessel and the second holding vessel, and described second The side of holding vessel is connected with third conveying pipeline, and the side of the third conveying pipeline is equipped with water bath heating device, and the water-bath adds The side of thermal is connected with centrifuge, and the side of the centrifuge is connected with the first blending tank, and the one of first blending tank Side is equipped with fixing pipe, and the side of fixing pipe is connected to the side of the first holding vessel, and first blending tank is connected with absorption Device, the side of the absorber are connected with elution tank, and the side of the elution tank is connected with silica gel column chromatography device, the layer of silica gel The side of analysis column device is connected with the second blending tank, and the side of second blending tank is connected with Centrifugal concentrators, and described second is mixed The other side for closing tank is connected with NaOH solution tank NaOH, and the side of the Centrifugal concentrators is connected with drier.

Preferably, first feeding device includes the cleaning case that feed pipe lower end is arranged in, and the lower end of feed pipe is passed through It wears and extends in cleaning case, the side of the cleaning case is equipped with water pump, and the side of water pump is connected with the first water inlet pipe, and the The lower end of one water inlet pipe, which is run through and used, to be extended in cleaning case, and the other side of the cleaning case, which is run through, is equipped with the first conveying pipeline, and For first water inlet pipe between feed pipe and the first conveying pipeline, the upper end of first conveying pipeline is fixed with the first driving motor, The output shaft of first driving motor is through the first conveying pipeline and extends in the first conveying pipeline, the output of the first driving motor Shaft end is fixed with the first spiral conveying bar, and the lower end of the first spiral conveying bar is successively worn the first conveying pipeline, cleaning case and turned The upper end side that crush box is arranged in, institute are run through in the dynamic bottom being connected in cleaning case, the upper end side of first conveying pipeline The lower end for stating cleaning case is equipped with drainpipe.

Preferably, the grinding device includes the second driving motor for being fixed on crush box upper end, the second driving electricity The output shaft of machine is through crush box and extends in crush box, and the output shaft end of second driving motor is fixed with the second spiral shell Revolve conveying bar, and the lower end of the second spiral conveying bar bottom and extends to the lower end of crush box in the crush box, described second The lower end of spiral conveying bar is fixed with first gear, rotating bar that there are two the lower rotation sockets in the crush box, and second Spiral conveying bar is located between two rotating bars, and the lower end of two rotating bars is respectively and fixedly provided with second gear, and two second gears are equal It is meshed with first gear, the upper end of two rotating bars is equidistantly fixed with multiple crushing knifes, and the other side of the water pump connects It is connected to the second water inlet pipe, the lower end of second water inlet pipe is run through the upper end of crush box and extended in crush box, described second The lower end of water inlet pipe is fixed with connecting tube, and the upper end of connecting tube is fixed on crush box inner top, the lower end etc. of the connecting tube Spacing is equipped with multiple spray heads, and second driving motor is between the first conveying pipeline and the second water inlet pipe.

Preferably, the filter device includes the 5th driving motor, is all provided in the opposing sidewalls in second conveying pipeline There is gap, link block is mounted in the gap, be fixed with filter plate between two link blocks jointly, under the link block End side is rotatablely connected there are two the polished rod being parallel to each other, and the lower end of the polished rod is run through equipped with casing, on the polished rod It is arranged with spring, and spring is located in casing, the upper end of the spring is fixed on the top in casing, and the lower end of the spring is solid It is scheduled on the lower end of polished rod, the 5th driving motor is mounted on the side of the second conveying pipeline, the output of the 5th driving motor Axis is through the second conveying pipeline and extends in the second conveying pipeline, and the output shaft end bias of the 5th driving motor, which is fixed with, to be turned Movable plate, and the upper end of rotor plate and the lower end of filter plate are inconsistent, the lower end of second conveying pipeline, which is run through, is arranged in shell Upper end side.

Preferably, the mixing arrangement includes the third driving motor for being mounted on shell side, third driving motor it is defeated Shaft runs through the side of shell and extends in shell, and the output shaft end of the third driving motor is fixed with the first stirring Axis, one end of first agitating shaft are rotatably connected on the intracorporal one end side wall of shell, equidistantly set on first agitating shaft There are multiple first paddles, the upper end other side of the shell is fixed with cellulase storage bin, the cellulase storage bin Lower end run through and extend in shell.

Preferably, the water bath heating device includes through the heating tank that third conveying pipeline one end is arranged in, the heating It is equipped with heating chamber in tank, is equipped with mixing chamber in the heating tank, and mixing chamber is located in heating chamber, the lower end of the heating tank is set There is secondary heater, the lower end quadrangle of the heating tank is respectively and fixedly provided with second support, and secondary heater is located at four second Between frame, the upper end of the heating tank is fixed with the 4th driving motor, and the output shaft end of the 4th driving motor, which runs through, to be added Hot tank simultaneously extends in mixing chamber, and the output shaft end of the 4th driving motor is fixed with the second agitating shaft, and the second stirring The lower end of axis is rotatably connected on the bottom in stir chamber, is equidistantly equipped with multiple second paddles, institute on second agitating shaft The upper end side for stating heating tank is fixed with ether holding vessel, and the lower end of the ether holding vessel through heating tank and extends to mixing It is intracavitary.

Preferably, described sleeve pipe is cylinder closed at one end, and described sleeve pipe is closed end close to one end of link block.

Preferably, the side of the cleaning case is fixed on the lower end side of crush box, under the cleaning case and crush box End is fixed with first support jointly, and shell is located in first support.

The invention also provides a kind of from STEVIA REBAUDIANA extracts the extracting method of stevioside, comprising the following steps:

S1 picks STEVIA REBAUDIANA, and STEVIA REBAUDIANA is sent by feed pipe to cleaning case, and water pump passes through the first water inlet pipe to clear Tank-washer inner water-supply cleans the STEVIA REBAUDIANA in cleaning case, and waste water is drained by drainpipe；

S2, the first driving motor drive the rotation of the first spiral conveying bar, the first spiral conveying bar rotation, by the sweet tea after cleaning Ye Jucong cleaning case is sent by the first conveying pipeline to crush box, and multiple through-holes, side are equidistantly equipped on the first spiral conveying bar Just water is exhausted；

S3, the second driving motor drive the second spiral conveying bar rotation, can effectively by the STEVIA REBAUDIANA of bottom in crush box from Bottom is sent to upper end in crush box, and the rotation of the second spiral conveying bar drives first gear rotation, and first gear rotation drives the The rotation of two gears, to make two rotating bar rotations, rotating bar rotation drives crushing knife rotation, by the sweetleaf of bottom in crush box Chrysanthemum carries out cutting and grinding, while the second spiral conveying bar takes up the STEVIA REBAUDIANA of bottom, can be effectively to the STEVIA REBAUDIANA in crush box It is crushed, water pump is convenient for crushing by water spray in the second water inlet pipe, spray head phase crush box；

S4, juice, powder after cutting are driven by water flows into shell, while the 5th driving motor band turn by filter plate Movable plate rotation, rotor plate eccentric rotary goes up and down filter plate, while being vibrated under the action of spring, casing and polished rod, just Shell is entered by filter plate in the STEVIA REBAUDIANA lower than 100 mesh；

S5 launches cellulase into shell by cellulase storage bin, and cellulase is the 0.1 of the water volume being added ~0.3w/v%, cellulase are mixed under the action of the first paddle with smashed STEVIA REBAUDIANA and water, third driving electricity Machine drive the first agitating shaft rotation, power is provided, mixing quality is effectively improved, thus improve wait until STEVIA REBAUDIANA enzymolysis liquid Quality；S6, obtained STEVIA REBAUDIANA enzymolysis liquid enter primary heater and carry out microwave heating, be delivered to after being heated to 35 ° -40 ° Ultrasonic Pulverization is carried out in ultrasonic generator, is filtered after 40-50 minute ultrasonic, and filter residue is into the second holding vessel, and filtrate is into the One holding vessel；S7, filter residue is delivered to the mixing chamber in heating tank by third conveying pipeline from the second holding vessel, while ether stores Injection ether of the tank into mixing chamber, makes the mass ratio 1:3-5 of filter residue and ether, secondary heater to the water in heating chamber into Row heating, the 4th driving motor drive the second agitating shaft to rotate the second paddle, are stirred, are allowed to uniformly be heated, It is delivered in centrifuge and is separated after heating, the rate of centrifugation is 2500-3500r/ minutes, and the time of centrifugation is 80-100 points Clock collects supernatant liquor after standing 20-30 minute, and by supernatant liquor be delivered in the first blending tank in the first holding vessel Filtrate is mixed；S8, mixed liquid are delivered to absorber and carry out decolorization adsorption, then are delivered to elution tank and are washed with eluent De-, eluent uses mass fraction for the ethyl alcohol of 65-75%, is delivered in silica gel column chromatography device and is separated after elution, separates The solution that liquid afterwards enters in the second blending tank and NaOH solution tank NaOH is delivered to Centrifugal concentrators and carries out centrifugal concentrating after being mixed, Solution in NaOH solution tank NaOH uses the calcium hydroxide that mass fraction is 20-30% for the liquor ferri trichloridi of 20-30%, mass fraction The mixed liquor of solution is uniformly mixed with the liquid in the second blending tank using the volume ratio of 3-10:50-80, mixing liquid into from It after heart inspissator is filtered, is centrifuged, being concentrated, then is delivered to drier and is dried, obtain stevioside.

Compared with prior art, the beneficial effects of the present invention are:

It 1, can be effectively in cleaning case by the cooperation of the first driving motor, the first spiral conveying bar and the first water inlet pipe STEVIA REBAUDIANA clear up and cleaned STEVIA REBAUDIANA is sent in crush box, it is inconvenient to solve prior art chinese raw materials The problem of being cleared up, being easy to appear impurity residual, has achieved the purpose that effectively to clean raw material, reduces on raw material The residual of impurity, to improve the quality of final products；

2, by the cooperation of the second driving motor, the second spiral conveying bar and rotating bar, enable crushing knife quick rotation simultaneously The STEVIA REBAUDIANA of bottom can be moved up, solve the problems, such as to take rate lower in the prior art, reached the mesh for effectively improving recovery rate , effectively STEVIA REBAUDIANA can be crushed, thus facilitate it is subsequent handled, improve processing quality；

3, by the cooperation of the 5th driving motor, rotor plate, spring, casing and polished rod, so that filter plate is stablized and shake It is dynamic, it solves the problems, such as that raw material utilization rate is low in the prior art, has achieved the purpose that efficient using raw material, improve the use of raw material Rate, so as to effectively improve the recovery rate of stevioside；

It 4, can water in heating chamber by the cooperation of secondary heater, heating tank, the 4th driving motor and the second agitating shaft It is heated, and rotates the second paddle, to be evenly heated to the liquid in energy mixing chamber, solve the prior art Middle excessive temperature destroys the problem of the activity of stevioside, has reached the active purpose of protection stevioside, so as to Improve product quality；

In conclusion the present invention can effectively clean raw material, impurity residual is reduced, convenient for carrying out STEVIA REBAUDIANA It crushes, and can be carried out screening, reacted convenient for subsequent, improve recovery rate, and guarantee the activity of stevioside, improve product Quality.

Detailed description of the invention

Fig. 1 is a kind of crush box structural representation of device that stevioside is extracted from STEVIA REBAUDIANA proposed by the present invention Figure；

Fig. 2 is a kind of heating tank structural representation of device that stevioside is extracted from STEVIA REBAUDIANA proposed by the present invention Figure；

Fig. 3 is that a kind of the second conveying pipeline structure of device that stevioside is extracted from STEVIA REBAUDIANA proposed by the present invention is shown It is intended to；

Fig. 4 is a kind of cannula structure schematic diagram of device that stevioside is extracted from STEVIA REBAUDIANA proposed by the present invention；

Fig. 5 is a kind of filter plate structural representation of device that stevioside is extracted from STEVIA REBAUDIANA proposed by the present invention Figure；Fig. 6 is a kind of step block diagram of extracting method that stevioside is extracted from STEVIA REBAUDIANA proposed by the present invention.

In figure: 1 feed pipe, 2 cleaning cases, 3 drainpipes, 4 brackets, 5 first conveying pipelines, 6 first spiral conveying bars, 7 first Water inlet pipe, 8 first driving motors, 9 second driving motors, 10 second water inlet pipes, 11 connecting tubes, 12 spray heads, 13 second spiral transferrings Expect bar, 14 crush boxes, 15 crushing knifes, 16 rotating bars, 17 first gears, 18 second gears, 19 cellulase storage bins, 20 shells Body, 21 first agitating shafts, 22 first paddles, 23 third driving motors, 24 gap, 25 link blocks, 26 filter plates, 27 rotations Plate, 28 second conveying pipelines, 29 primary heaters, 30 ultrasonic generators, 31 first holding vessels, 32 second holding vessels, the storage of 33 ether Deposit tank, 34 the 4th driving motors, 35 third conveying pipelines, 36 impellers, 37 second supports, 38 secondary heaters, 39 second paddles, 40 second agitating shafts, 41 centrifuges, 42 first blending tanks, 43 polished rods, 44 casings, 45 springs, 46 the 5th driving motors, 47 water Pump, 48 absorbers, 49 elution tanks, 50 silica gel column chromatography devices, 51 second blending tanks, 52 Centrifugal concentrators, 53 NaOH solution tank NaOHs, 54 dryings Device, 55 heating tanks.

Specific embodiment

Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Implement Example 1:

Referring to Fig.1-6, a kind of extracting method for extracting stevioside from STEVIA REBAUDIANA, comprising the following steps:

S1 picks STEVIA REBAUDIANA, and STEVIA REBAUDIANA is sent by feed pipe 1 to cleaning case 2, and water pump 47 passes through the first water inlet pipe 7, to 2 inner water-supply of cleaning case, clean the STEVIA REBAUDIANA in cleaning case 2, and waste water is drained by drainpipe 3, waste water and miscellaneous pass through Cleaning case 2 is discharged in drainpipe 3；

S2, the first driving motor 8 drive the rotation of the first spiral conveying bar 6, and the first driving motor 8 uses HVP280M model, Output is stablized, and the first spiral conveying bar 6 rotation, the first driving motor 8 drives the rotation of the first spiral conveying bar 6, after cleaning STEVIA REBAUDIANA is sent by the first conveying pipeline 5 to crush box 14 from cleaning case 2, is equidistantly equipped on the first spiral conveying bar 6 multiple Through-hole facilitates and exhausts water, can effectively reduce into the impurity in crush box 14；

S3, the second driving motor 9 drive the rotation of the second spiral conveying bar 13, and the second driving motor 9 uses HVP280M model The STEVIA REBAUDIANA of bottom in crush box 14 effectively can be sent to upper end in crush box 14,9 band of the second driving motor from bottom by model Dynamic second spiral conveying bar 13 rotation, it is other so as to which the upper end of crush box 14 will be moved in the STEVIA REBAUDIANA of bottom in crush box 14 The mobile filling of the STEVIA REBAUDIANA on side, makes STEVIA REBAUDIANA form circulation in crush box 14, and the rotation of the second spiral conveying bar 13 drives first Gear 17 rotates, and the rotation of first gear 17 drives second gear 18 to rotate, so that two rotating bars 16 are rotated, 16 turns of rotating bar It is dynamic that crushing knife 15 is driven to rotate, the STEVIA REBAUDIANA of bottom in crush box 14 is subjected to cutting and grinding, while the second spiral conveying bar 13 The STEVIA REBAUDIANA of bottom is taken up, effectively the STEVIA REBAUDIANA in crush box 14 can be crushed, STEVIA REBAUDIANA is formed in crush box 14 Circulation, so as to make crushing knife 15 carry out effective cutting and grinding to STEVIA REBAUDIANA, water pump 47 passes through the second water inlet pipe 10,12 phase of spray head Water spray, is convenient for crushing in crush box 14, and water pump 47 is by the second water inlet pipe 10 and spray head 12 to spraying in crush box 14 Water while convenient for crushing, flows the STEVIA REBAUDIANA juice after being crushed and powder downstream；

S4, juice, powder after cutting are driven by water flows into shell 20, while the 5th driving motor 46 by filter plate 26 Rotor plate 27 is driven to rotate, the 5th driving motor 46 uses HVP280M type, and 27 eccentric rotary of rotor plate goes up and down filter plate 26, It being vibrated under the action of spring 45, casing 44 and polished rod 43 simultaneously, filter plate 26 rises under the action of rotor plate 27, from So that polished rod 43 presses to spring 45, shrink spring 45, spring 45 generates the power restored, and rotor plate 27 continues to rotate, spring 45 pull filter plate 26 to reset, to make filter plate 26 shake, convenient for screening to the STEVIA REBAUDIANA of cutting and grinding, convenient for being lower than The STEVIA REBAUDIANA of 100 mesh enters shell 20 by filter plate 26, facilitates and is mixed；

S5 launches cellulase into shell 20 by cellulase storage bin 19, and cellulase is the water volume being added 0.2w/v%, cellulase mixed under the action of the first paddle 22 with smashed STEVIA REBAUDIANA and water, third driving Motor 23 drives the rotation of the first agitating shaft 21, and third driving motor 23 uses HVP280M type, provides power, and effective raising is mixed Quality is closed, to improve the quality for obtaining STEVIA REBAUDIANA enzymolysis liquid waited until；S6, obtained STEVIA REBAUDIANA enzymolysis liquid enter the first heating Device 29 carries out microwave heating, is delivered in ultrasonic generator 30 after being heated to 38 ° and carries out Ultrasonic Pulverization, and ultrasound carries out after 48 minutes Filtering, filter residue enter the second holding vessel 32, and filtrate is into the first holding vessel 31；S7, filter residue are defeated by third from the second holding vessel 32 Expects pipe 35 is delivered to the mixing chamber in heating tank 55, while injection ether of the ether holding vessel 33 into mixing chamber, make filter residue and The mass ratio of ether is 1:4, and secondary heater 38 heats the water in heating chamber, and the 4th driving motor 34 drives second to stir Mixing axis 40 rotates the second paddle 39, is stirred, is allowed to uniformly be heated, and is delivered in centrifuge 41 and carries out after heating Separation, the rate of centrifugation are 3000r/ minutes, and the time of centrifugation is 90 minutes, supernatant liquor are collected after standing 28 minutes, and will The filtrate that supernatant liquor is delivered in the first blending tank 42 and in the first holding vessel 31 is mixed；S8, mixed liquid are defeated Send to absorber 48 carry out decolorization adsorption, then be delivered to elution the elution of tank 49, eluent use mass fraction for 70% ethyl alcohol is delivered in silica gel column chromatography device 50 after elution and is separated, and the liquid after separation enters the second blending tank 51 It is delivered to Centrifugal concentrators 52 after being mixed with the solution in NaOH solution tank NaOH 53 and carries out centrifugal concentrating, the solution in NaOH solution tank NaOH 53 is adopted The mixed liquor for the calcium hydroxide solution that the liquor ferri trichloridi for being 25% with mass fraction, mass fraction are 25%, it is mixed with second The liquid closed in tank 51 is uniformly mixed using the volume ratio of 8:68, and mixing liquid is filtered into Centrifugal concentrators 52, is centrifuged, is dense It after contracting, then is delivered to drier 54 and is dried, obtain stevioside.A kind of dress extracting stevioside from STEVIA REBAUDIANA It sets, including feed pipe 1, the side of feed pipe 1 is equipped with the first feeding device, and the first feeding device is equipped with crush box 14, and first Feeding device can be by feedstock transportation to crush box 14, and crush box 14 is equipped with grinding device, raw material is crushed, crush box 14 lower end side run through be equipped with the second conveying pipeline 28, the second conveying pipeline 28 be equipped with filter device, by smashed raw material into Row filtering selects qualified raw material in next step, can more preferably be reacted；Filter device is equipped with shell 20, in shell 20 It equipped with mixing arrangement, can effectively be mixed, the side of mixing arrangement is equipped with primary heater 29, facilitates and is heated, and improves Mixing efficiency, the side of primary heater 29 are connected with ultrasonic generator 30, and the side of ultrasonic generator 30 is connected with the first storage Tank 31 and the second holding vessel 32 are deposited, classified storage is facilitated, the side of the second holding vessel 32 is connected with third conveying pipeline 35, and third is defeated The side of expects pipe 35 be equipped with water bath heating device, carry out heating water bath, guarantee activity, the side of water bath heating device be connected with from Scheming 41, the side of centrifuge 41 are connected with the first blending tank 42, and the side of the first blending tank 42 is equipped with fixing pipe, and fixing pipe Side be connected to the side of the first holding vessel 31, effectively mixed, after material is carried out reaction treatment, facilitate and carry out down The processing of one step, can improve reaction mass, to improve product quality；First blending tank 42 is connected with absorber 48, uses suction Adnexa 48 carries out adsorption bleaching, and the side of absorber 48 is connected with elution tank 49, and the side of elution tank 49 is connected with silica gel column chromatography Column device 50, the side of silica gel column chromatography device 50 are connected with the second blending tank 51, and it is dense that the side of the second blending tank 51 is connected with centrifugation Contracting device 52, the other side of the second blending tank 51 are connected with NaOH solution tank NaOH 53, and the side of Centrifugal concentrators 52 is connected with drier 54, warp Absorber carries out decolorization adsorption, and silica gel column chromatography post separation, elution are dried after centrifugal concentrating, to obtain sweetleaf Synanthrin glycosides guarantees product quality.In the present invention, the first feeding device includes the cleaning case 2 that 1 lower end of feed pipe is arranged in, and into The lower end of expects pipe 1 is run through and is extended in cleaning case 2, and raw material is entered in cleaning case 2 by feed pipe 1, and the side of cleaning case 2 is solid It is scheduled on the lower end side of crush box 14, the lower end of cleaning case 2 and crush box 14 is fixed with first support 4, and shell 20 jointly In in first support 4, the side of cleaning case 2 is equipped with water pump 47, and the side of water pump 47 is connected with the first water inlet pipe 7, and first The lower end of water inlet pipe 7, which is run through and used, to be extended in cleaning case 2, and water pump 47 is filled the water by the first water inlet pipe 7 into cleaning case 2, right Raw material is effectively cleaned, and is guaranteed the cleaning of raw material, is conducive to subsequent reactions: the other side of cleaning case 2 runs through defeated equipped with first Expects pipe 5, and the first water inlet pipe 7 is located between feed pipe 1 and the first conveying pipeline 5, the upper end of the first conveying pipeline 5 is fixed with the first drive Dynamic motor 8 stablizes output, facilitates and controlled, and the output shaft of the first driving motor 8 is through the first conveying pipeline 5 and extends to the In one conveying pipeline 5, the output shaft end of the first driving motor 8 is fixed with the first spiral conveying bar 6, and the first spiral conveying bar 6 Lower end successively wear the first conveying pipeline 5, cleaning case 2 and the bottom being rotatably connected in cleaning case 2, the first driving motor 8 drives The rotation of first spiral conveying bar 6 facilitates and is delivered to raw material in crush box 14 by the first conveying pipeline 5 from cleaning case 2, and first The upper end side that crush box 14 is arranged in is run through in the upper end side of conveying pipeline 5, and the lower end of cleaning case 2 is equipped with drainpipe 3, can will give up Water is quickly discharged, and guarantees cleaning effect, to be conducive to subsequent reactions, guarantees the quality of production product.In the present invention, dress is crushed The second driving motor 9 including being fixed on 14 upper end of crush box is set, the output shaft of the second driving motor 9 through crush box 14 and prolongs It extends in crush box 14, the output shaft end of the second driving motor 9 is fixed with the second spiral conveying bar 13, and the second spiral conveying The lower end of bar 13 bottom and extends to the lower end of crush box 14 in the crush box 14, and the second driving motor 9 can drive the second spiral shell It revolves conveying bar 13 to rotate, raw material can be sent to the top in crush box 14 from the bottom in crush box 14, so that raw material be made to exist Circulation is formed in crush box 14, is improved and is crushed quality；The lower end of second spiral conveying bar 13 is fixed with first gear 17, crush box There are two rotating bars 16 for lower rotation socket in 14, and the second spiral conveying bar 13 is located between two rotating bars 16, and two The lower end of rotating bar 16 is respectively and fixedly provided with second gear 18, and two second gears 18 are meshed with first gear 17, the second spiral The rotation of conveying bar 13 can drive first gear 17 to rotate, and the rotation of first gear 17 will drive two second gears 18 and rotate, and two The upper end of rotating bar 16 is equidistantly fixed with multiple crushing knifes 15, and the rotation of second gear 18 can be driven by rotating bar 16 and be crushed Knife 15 rotates, and crushes to raw material, the other side of water pump 47 is connected with the second water inlet pipe 10, and the lower end of the second water inlet pipe 10 is passed through It wears the upper end of crush box 14 and extends in crush box 14, the lower end of the second water inlet pipe 10 is fixed with connecting tube 11, and connecting tube 11 upper end is fixed on 14 inner top of crush box, and the lower end of connecting tube 11 is equidistantly equipped with multiple spray heads 12, and water pump 47 can pass through Second water inlet pipe 10 is sprayed water into crush box 14, and smashed raw material is facilitated to flow movement downstream, and the second driving motor 9 is located at first Between conveying pipeline 5 and the second water inlet pipe 10, guarantee to crush quality, so as to improve the quality of subsequent reactions.In the present invention, filtering Device includes the 5th driving motor 46, is equipped with gap 24 in the opposing sidewalls in the second conveying pipeline 28, is respectively mounted in gap 24 There is link block 25, be fixed with filter plate 26 between two link blocks 25 jointly, link block 25 to filter plate 26 is steadily mobile, connection The lower end side of block 25 is rotatablely connected there are two the polished rod 43 being parallel to each other, and the lower end of polished rod 43 is run through equipped with casing 44, Casing 44 is cylinder closed at one end, and casing 44 is closed end close to one end of link block 25, facilitates the movement of polished rod 43, polished rod Spring 45 is arranged on 43, and spring 45 is located in casing 44, the upper end of spring 45 is fixed on the top in casing 44, spring 45 Lower end be fixed on the lower end of polished rod 43, the mobile polished rod 43 that will push of link block 25 is mobile, to make spring 45, deformation occurs, bullet Spring 45 generates the power restored, and shakes convenient for filter plate 26, to conveniently be filtered；It is defeated that 5th driving motor 46 is mounted on second The output shaft of the side of expects pipe 28, the 5th driving motor 46 through the second conveying pipeline 28 and extends in the second conveying pipeline 28, the The output shaft end bias of five driving motors 46 is fixed with rotor plate 27, and the lower end phase of the upper end of rotor plate 27 and filter plate 26 It contradicts, the 5th driving motor 46 drives rotor plate 27 to rotate, to shake for filter plate 26, to conveniently be filtered, second The upper end side that shell 20 is arranged in is run through in the lower end of conveying pipeline 28, facilitates conveying.In the present invention, mixing arrangement includes being mounted on The third driving motor 23 of 20 side of shell stablizes output, is convenient for controlling, and the output shaft of third driving motor 23 runs through shell The side of body 20 simultaneously extends in shell 20, and the output shaft end of third driving motor 23 is fixed with the first agitating shaft 21, third Driving motor 23 drives the rotation of the first agitating shaft 21, and one end of the first agitating shaft 21 is rotatably connected on one end side wall in shell 20 On, multiple first paddles 22 are equidistantly equipped on the first agitating shaft 21, the rotation of the first agitating shaft 21 drives the first paddle 22 Rotation, is stirred, the upper end other side of shell 20 is fixed with cellulase storage bin 19, the lower end of cellulase storage bin 19 Run through and extend in shell 20, makes to be uniformly mixed between material, be conducive to subsequent reactions.In the present invention, water bath heating device Including being equipped with heating chamber in the heating tank 55 that 35 one end of third conveying pipeline is arranged in, heating tank 55, it is equipped in heating tank 55 Mixing chamber, and mixing chamber is located in heating chamber, it is convenient that the material in mixing chamber is heated by heating chamber, heating tank 55 Lower end is equipped with secondary heater 38, and the lower end quadrangle of heating tank 55 is respectively and fixedly provided with second support 37, and secondary heater 38 is located at Between four second supports 37, stablize support, the upper end of heating tank 55 is fixed with the 4th driving motor 34, the 4th driving motor 34 Output shaft end through heating tank 55 and extending in mixing chamber, the output shaft end of the 4th driving motor 34 is fixed with second Agitating shaft 40, and the lower end of the second agitating shaft 40 is rotatably connected on the bottom in stir chamber, the 4th driving motor 34 drives second Agitating shaft 40 rotates, and multiple second paddles 39 are equidistantly equipped on the second agitating shaft 40, and the second agitating shaft 40 drives second to stir The rotation of leaf 39 is mixed, is stirred, is heated properly material, the upper end side of heating tank 55 is fixed with ether holding vessel 33, second The lower end of ether holding vessel 33 is through heating tank 55 and extends in mixing chamber, conveniently knocks down material, is evenly heated, advantageously ensures that Product Activity improves product quality.

Embodiment 2:

S5 launches cellulase into shell 20 by cellulase storage bin 19, and cellulase is the water volume being added 0.25w/v%, cellulase mixed under the action of the first paddle 22 with smashed STEVIA REBAUDIANA and water, and third is driven Dynamic motor 23 drives the rotation of the first agitating shaft 21, and third driving motor 23 uses HVP280M type, provides power, effective to improve Mixing quality, to improve the quality for obtaining STEVIA REBAUDIANA enzymolysis liquid waited until；S6, obtained STEVIA REBAUDIANA enzymolysis liquid enter the first heating Device 29 carries out microwave heating, is delivered in ultrasonic generator 30 after being heated to 32 ° and carries out Ultrasonic Pulverization, and ultrasound carries out after 45 minutes Filtering, filter residue enter the second holding vessel 32, and filtrate is into the first holding vessel 31；S7, filter residue are defeated by third from the second holding vessel 32 Expects pipe 35 is delivered to the mixing chamber in heating tank 55, while injection ether of the ether holding vessel 33 into mixing chamber, make filter residue and The mass ratio of ether is 1:3.5, and secondary heater 38 heats the water in heating chamber, and the 4th driving motor 34 drives second Agitating shaft 40 rotates the second paddle 39, is stirred, is allowed to uniformly be heated, be delivered to after heating in centrifuge 41 into Row separation, the rate of centrifugation are 2800r/ minutes, and the time of centrifugation is 85 minutes, collect supernatant liquor after standing 25 minutes, and The filtrate that supernatant liquor is delivered in the first blending tank 42 and in the first holding vessel 31 is mixed；S8, mixed liquid Be delivered to absorber 48 carry out decolorization adsorption, then be delivered to elution the elution of tank 49, eluent use mass fraction for 68% ethyl alcohol is delivered in silica gel column chromatography device 50 after elution and is separated, and the liquid after separation enters the second blending tank 51 It is delivered to Centrifugal concentrators 52 after being mixed with the solution in NaOH solution tank NaOH 53 and carries out centrifugal concentrating, the solution in NaOH solution tank NaOH 53 is adopted The mixed liquor for the calcium hydroxide solution that the liquor ferri trichloridi for being 23% with mass fraction, mass fraction are 23%, it is mixed with second The liquid closed in tank 51 is uniformly mixed using the volume ratio of 5:55, and mixing liquid is filtered into Centrifugal concentrators 52, is centrifuged, is dense It after contracting, then is delivered to drier 54 and is dried, obtain stevioside.

Embodiment 3:

S5 launches cellulase into shell 20 by cellulase storage bin 19, and cellulase is the water volume being added 0.15w/v%, cellulase mixed under the action of the first paddle 22 with smashed STEVIA REBAUDIANA and water, and third is driven Dynamic motor 23 drives the rotation of the first agitating shaft 21, and third driving motor 23 uses HVP280M type, provides power, effective to improve Mixing quality, to improve the quality for obtaining STEVIA REBAUDIANA enzymolysis liquid waited until；S6, obtained STEVIA REBAUDIANA enzymolysis liquid enter the first heating Device 29 carries out microwave heating, is delivered in ultrasonic generator 30 after being heated to 36 ° and carries out Ultrasonic Pulverization, and ultrasound carries out after 43 minutes Filtering, filter residue enter the second holding vessel 32, and filtrate is into the first holding vessel 31；S7, filter residue are defeated by third from the second holding vessel 32 Expects pipe 35 is delivered to the mixing chamber in heating tank 55, while injection ether of the ether holding vessel 33 into mixing chamber, make filter residue and The mass ratio of ether is 1:3.2, and secondary heater 38 heats the water in heating chamber, and the 4th driving motor 34 drives second Agitating shaft 40 rotates the second paddle 39, is stirred, is allowed to uniformly be heated, be delivered to after heating in centrifuge 41 into Row separation, the rate of centrifugation are 2600r/ minutes, and the time of centrifugation is 82 minutes, collect supernatant liquor after standing 22 minutes, and The filtrate that supernatant liquor is delivered in the first blending tank 42 and in the first holding vessel 31 is mixed；S8, mixed liquid Be delivered to absorber 48 carry out decolorization adsorption, then be delivered to elution the elution of tank 49, eluent use mass fraction for 66% ethyl alcohol is delivered in silica gel column chromatography device 50 after elution and is separated, and the liquid after separation enters the second blending tank 51 It is delivered to Centrifugal concentrators 52 after being mixed with the solution in NaOH solution tank NaOH 53 and carries out centrifugal concentrating, the solution in NaOH solution tank NaOH 53 is adopted The mixed liquor for the calcium hydroxide solution that the liquor ferri trichloridi for being 22% with mass fraction, mass fraction are 22%, it is mixed with second The liquid closed in tank 51 is uniformly mixed using the volume ratio of 3:50, and mixing liquid is filtered into Centrifugal concentrators 52, is centrifuged, is dense It after contracting, then is delivered to drier 54 and is dried, obtain stevioside.

Embodiment 4:

S5 launches cellulase into shell 20 by cellulase storage bin 19, and cellulase is the water volume being added 0.28w/v%, cellulase mixed under the action of the first paddle 22 with smashed STEVIA REBAUDIANA and water, and third is driven Dynamic motor 23 drives the rotation of the first agitating shaft 21, and third driving motor 23 uses HVP280M type, provides power, effective to improve Mixing quality, to improve the quality for obtaining STEVIA REBAUDIANA enzymolysis liquid waited until；S6, obtained STEVIA REBAUDIANA enzymolysis liquid enter the first heating Device 29 carries out microwave heating, is delivered in ultrasonic generator 30 after being heated to 39 ° and carries out Ultrasonic Pulverization, and ultrasound carries out after 49 minutes Filtering, filter residue enter the second holding vessel 32, and filtrate is into the first holding vessel 31；S7, filter residue are defeated by third from the second holding vessel 32 Expects pipe 35 is delivered to the mixing chamber in heating tank 55, while injection ether of the ether holding vessel 33 into mixing chamber, make filter residue and The mass ratio of ether is 1:4.8, and secondary heater 38 heats the water in heating chamber, and the 4th driving motor 34 drives second Agitating shaft 40 rotates the second paddle 39, is stirred, is allowed to uniformly be heated, be delivered to after heating in centrifuge 41 into Row separation, the rate of centrifugation are 3400r/ minutes, and the time of centrifugation is 98 minutes, collect supernatant liquor after standing 28 minutes, and The filtrate that supernatant liquor is delivered in the first blending tank 42 and in the first holding vessel 31 is mixed；S8, mixed liquid Be delivered to absorber 48 carry out decolorization adsorption, then be delivered to elution the elution of tank 49, eluent use mass fraction for 73% ethyl alcohol is delivered in silica gel column chromatography device 50 after elution and is separated, and the liquid after separation enters the second blending tank 51 It is delivered to Centrifugal concentrators 52 after being mixed with the solution in NaOH solution tank NaOH 53 and carries out centrifugal concentrating, the solution in NaOH solution tank NaOH 53 is adopted The mixed liquor for the calcium hydroxide solution that the liquor ferri trichloridi for being 28% with mass fraction, mass fraction are 28%, it is mixed with second The liquid closed in tank 51 is uniformly mixed using the volume ratio of 9:75, and mixing liquid is filtered into Centrifugal concentrators 52, is centrifuged, is dense It after contracting, then is delivered to drier 54 and is dried, obtain stevioside.

Embodiment 4:

S5 launches cellulase into shell 20 by cellulase storage bin 19, and cellulase is the water volume being added 0.12w/v%, cellulase mixed under the action of the first paddle 22 with smashed STEVIA REBAUDIANA and water, and third is driven Dynamic motor 23 drives the rotation of the first agitating shaft 21, and third driving motor 23 uses HVP280M type, provides power, effective to improve Mixing quality, to improve the quality for obtaining STEVIA REBAUDIANA enzymolysis liquid waited until；S6, obtained STEVIA REBAUDIANA enzymolysis liquid enter the first heating Device 29 carries out microwave heating, is delivered in ultrasonic generator 30 after being heated to 36 ° and carries out Ultrasonic Pulverization, and ultrasound carries out after 42 minutes Filtering, filter residue enter the second holding vessel 32, and filtrate is into the first holding vessel 31；S7, filter residue are defeated by third from the second holding vessel 32 Expects pipe 35 is delivered to the mixing chamber in heating tank 55, while injection ether of the ether holding vessel 33 into mixing chamber, make filter residue and The mass ratio of ether is 1:3.2, and secondary heater 38 heats the water in heating chamber, and the 4th driving motor 34 drives second Agitating shaft 40 rotates the second paddle 39, is stirred, is allowed to uniformly be heated, be delivered to after heating in centrifuge 41 into Row separation, the rate of centrifugation are 2600r/ minutes, and the time of centrifugation is 82 minutes, collect supernatant liquor after standing 22 minutes, and The filtrate that supernatant liquor is delivered in the first blending tank 42 and in the first holding vessel 31 is mixed；S8, mixed liquid Be delivered to absorber 48 carry out decolorization adsorption, then be delivered to elution the elution of tank 49, eluent use mass fraction for 66% ethyl alcohol is delivered in silica gel column chromatography device 50 after elution and is separated, and the liquid after separation enters the second blending tank 51 It is delivered to Centrifugal concentrators 52 after being mixed with the solution in NaOH solution tank NaOH 53 and carries out centrifugal concentrating, the solution in NaOH solution tank NaOH 53 is adopted The mixed liquor for the calcium hydroxide solution that the liquor ferri trichloridi for being 21% with mass fraction, mass fraction are 21%, it is mixed with second The liquid closed in tank 51 is uniformly mixed using the volume ratio of 3:52, and mixing liquid is filtered into Centrifugal concentrators 52, is centrifuged, is dense It after contracting, then is delivered to drier 54 and is dried, obtain stevioside.

Embodiment 5:

S5 launches cellulase into shell 20 by cellulase storage bin 19, and cellulase is the water volume being added 0.28w/v%, cellulase mixed under the action of the first paddle 22 with smashed STEVIA REBAUDIANA and water, and third is driven Dynamic motor 23 drives the rotation of the first agitating shaft 21, and third driving motor 23 uses HVP280M type, provides power, effective to improve Mixing quality, to improve the quality for obtaining STEVIA REBAUDIANA enzymolysis liquid waited until；S6, obtained STEVIA REBAUDIANA enzymolysis liquid enter the first heating Device 29 carries out microwave heating, is delivered in ultrasonic generator 30 after being heated to 39 ° and carries out Ultrasonic Pulverization, and ultrasound carries out after 49 minutes Filtering, filter residue enter the second holding vessel 32, and filtrate is into the first holding vessel 31；S7, filter residue are defeated by third from the second holding vessel 32 Expects pipe 35 is delivered to the mixing chamber in heating tank 55, while injection ether of the ether holding vessel 33 into mixing chamber, make filter residue and The mass ratio of ether is 1:4.8, and secondary heater 38 heats the water in heating chamber, and the 4th driving motor 34 drives second Agitating shaft 40 rotates the second paddle 39, is stirred, is allowed to uniformly be heated, be delivered to after heating in centrifuge 41 into Row separation, the rate of centrifugation are 3400r/ minutes, and the time of centrifugation is 98 minutes, collect supernatant liquor after standing 28 minutes, and The filtrate that supernatant liquor is delivered in the first blending tank 42 and in the first holding vessel 31 is mixed；S8, mixed liquid Be delivered to absorber 48 carry out decolorization adsorption, then be delivered to elution the elution of tank 49, eluent use mass fraction for 74% ethyl alcohol is delivered in silica gel column chromatography device 50 after elution and is separated, and the liquid after separation enters the second blending tank 51 It is delivered to Centrifugal concentrators 52 after being mixed with the solution in NaOH solution tank NaOH 53 and carries out centrifugal concentrating, the solution in NaOH solution tank NaOH 53 is adopted The mixed liquor for the calcium hydroxide solution that the liquor ferri trichloridi for being 28% with mass fraction, mass fraction are 28%, it is mixed with second The liquid closed in tank 51 is uniformly mixed using the volume ratio of 9:78, and mixing liquid is filtered into Centrifugal concentrators 52, is centrifuged, is dense It after contracting, then is delivered to drier 54 and is dried, obtain stevioside.Comparative example 1:

The stevioside that middle embodiment 1 is extracted in patent referring to CN105801639A.

Comparative example 2:

The stevioside that embodiment 1 is extracted in the patent of reference CN106632539A.

Comparative example 3:

The stevioside that embodiment 1 is extracted in the patent of CN105294790A.Comparative example 4:

A kind of extracting method for extracting stevioside from STEVIA REBAUDIANA, comprising the following steps:

S5 launches cellulase into shell 20 by cellulase storage bin 19, and cellulase is the water volume being added 0.13w/v%, cellulase mixed under the action of the first paddle 22 with smashed STEVIA REBAUDIANA and water, and third is driven Dynamic motor 23 drives the rotation of the first agitating shaft 21, and third driving motor 23 uses HVP280M type, provides power, effective to improve Mixing quality, to improve the quality for obtaining STEVIA REBAUDIANA enzymolysis liquid waited until；S6, obtained STEVIA REBAUDIANA enzymolysis liquid enter the first heating Device 29 carries out microwave heating, is delivered in ultrasonic generator 30 after being heated to 42 ° and carries out Ultrasonic Pulverization, and ultrasound carries out after 43 minutes Filtering, filter residue enter the second holding vessel 32, and filtrate is into the first holding vessel 31；S7, filter residue are defeated by third from the second holding vessel 32 Expects pipe 35 is delivered to the mixing chamber in heating tank 55, while injection ether of the ether holding vessel 33 into mixing chamber, make filter residue and The mass ratio of ether is 1:3.3, and secondary heater 38 heats the water in heating chamber, and the 4th driving motor 34 drives second Agitating shaft 40 rotates the second paddle 39, is stirred, is allowed to uniformly be heated, be delivered to after heating in centrifuge 41 into Row separation, the rate of centrifugation are 2700r/ minutes, and the time of centrifugation is 83 minutes, collect supernatant liquor after standing 24 minutes, and The filtrate that supernatant liquor is delivered in the first blending tank 42 and in the first holding vessel 31 is mixed；S8, mixed liquid Be delivered to absorber 48 carry out decolorization adsorption, then be delivered to elution the elution of tank 49, eluent use mass fraction for 67% ethyl alcohol is delivered in silica gel column chromatography device 50 after elution and is separated, and the liquid after separation enters the second blending tank 51 It is delivered to Centrifugal concentrators 52 after being mixed with the solution in NaOH solution tank NaOH 53 and carries out centrifugal concentrating, the solution in NaOH solution tank NaOH 53 is adopted The mixed liquor for the calcium hydroxide solution that the liquor ferri trichloridi for being 27% with mass fraction, mass fraction are 27%, it is mixed with second The liquid closed in tank 51 is uniformly mixed using the volume ratio of 4:60, and mixing liquid is filtered into Centrifugal concentrators 52, is centrifuged, is dense It after contracting, then is delivered to drier 54 and is dried, obtain stevioside.

Comparative example 5:

S5 launches cellulase into shell 20 by cellulase storage bin 19, and cellulase is the water volume being added 0.11w/v%, cellulase mixed under the action of the first paddle 22 with smashed STEVIA REBAUDIANA and water, and third is driven Dynamic motor 23 drives the rotation of the first agitating shaft 21, and third driving motor 23 uses HVP280M type, provides power, effective to improve Mixing quality, to improve the quality for obtaining STEVIA REBAUDIANA enzymolysis liquid waited until；S6, obtained STEVIA REBAUDIANA enzymolysis liquid enter the first heating Device 29 carries out microwave heating, is delivered in ultrasonic generator 30 after being heated to 31 ° and carries out Ultrasonic Pulverization, and ultrasound carries out after 41 minutes Filtering, filter residue enter the second holding vessel 32, and filtrate is into the first holding vessel 31；S7, filter residue are defeated by third from the second holding vessel 32 Expects pipe 35 is delivered to the mixing chamber in heating tank 55, while injection ether of the ether holding vessel 33 into mixing chamber, make filter residue and The mass ratio of ether is 1:3.1, and secondary heater 38 heats the water in heating chamber, and the 4th driving motor 34 drives second Agitating shaft 40 rotates the second paddle 39, is stirred, is allowed to uniformly be heated, be delivered to after heating in centrifuge 41 into Row separation, the rate of centrifugation are 2600r/ minutes, and the time of centrifugation is 81 minutes, collect supernatant liquor after standing 21 minutes, and The filtrate that supernatant liquor is delivered in the first blending tank 42 and in the first holding vessel 31 is mixed；S8, mixed liquid Be delivered to absorber 48 carry out decolorization adsorption, then be delivered to elution the elution of tank 49, eluent use mass fraction for 66% ethyl alcohol is delivered in silica gel column chromatography device 50 after elution and is separated, and the liquid after separation enters the second blending tank 51 It is delivered to Centrifugal concentrators 52 after being mixed with the solution in NaOH solution tank NaOH 53 and carries out centrifugal concentrating, the solution in NaOH solution tank NaOH 53 is adopted The mixed liquor for the calcium hydroxide solution that the liquor ferri trichloridi for being 21% with mass fraction, mass fraction are 21%, it is mixed with second The liquid closed in tank 51 is uniformly mixed using the volume ratio of 3:51, and mixing liquid is filtered into Centrifugal concentrators 52, is centrifuged, is dense It after contracting, then is delivered to drier 54 and is dried, obtain stevioside.Purity test: purity detecting: 1690FJ model is used High performance liquid chromatograph carry out rapid survey.Specific purity test the results are shown in Table 1:

Table 1, stevioside purity test result:

As can be seen from the above table, it is higher that stevioside purity is extracted in the embodiment of the present invention, it is effective without carrying out in comparative example 1 It crushes, so that purity is minimum, temperature is higher in extraction process in comparative example 2, to influence Product Activity, influences extraction Purity, so that impurity be made to enter extraction process, influences DNA purity not cleared up effectively in comparative example 3.Recovery rate is surveyed Examination: it recovery rate detection: is quickly tested by weighing.Specific recovery rate the results are shown in Table 2:

Table 2, stevioside recovery rate test result:

As can be seen from the above table, stevioside recovery rate is higher in the embodiment of the present invention, without carrying out effective powder in comparative example 1 Broken, so that recovery rate is minimum, temperature is higher in extraction process in comparative example 2, to influence Product Activity, influences product and mentions Rate is taken, is not cleared up effectively in comparative example 3, so that impurity be made to enter extraction process, influences product recovery rate.The present invention In, in use, STEVIA REBAUDIANA is knocked down in cleaning case 2 by feed pipe 1, water pump 47 is infused by the first water inlet pipe 7 into cleaning case 2 Water is rinsed, and by the discharge cleaning case 2 of drainpipe 3, the first driving motor 8 drives the first spiral conveying bar 6 for waste water and impurity Rotation, so as to which the STEVIA REBAUDIANA after rinsing is delivered to crush box 14, the second driving motor 9 passes through 13 band of the second spiral conveying bar Dynamic first gear 17 rotates, and first gear 17 rotates two second gears 18, so that crushing knife 15 be made to rotate, to STEVIA REBAUDIANA into Row crush, the second spiral conveying bar 13 rotation, the STEVIA REBAUDIANA of bottom can be moved up, so as to make the STEVIA REBAUDIANA in crush box 14 into Row circulation, to effectively improve crush efficiency, the 5th driving motor 46 drives rotor plate 27 to rotate, so that rotor plate 27 be made to push Filter plate 26 is gone up and down, and filter plate 26 is mobile to shrink spring 45 by polished rod 43, and spring 45 generates restoring force, convenient for driving filtering Plate 26 resets, and so as to make filter plate 26 generate vibration, is convenient for filtering screening, and shell 20 is entered after screening and is mixed, Microwave heating is carried out through primary heater 29 after mixing and 30 Ultrasonic Pulverization of ultrasonic generator filters, and filter residue enters the second holding vessel 32, filtrate enters the first holding vessel 31, and filter residue enters mixing chamber and ether is mixed, and centrifuge 41 is carried out after heating water bath Centrifugal screening, into the filtrate mixing in the first blending tank 42 and the first holding vessel 31, after through absorber carry out decolorization adsorption, silicon Glue-line analyses post separation, elution, is dried after centrifugal concentrating, to obtain stevioside.

More than, it is merely preferred embodiments of the present invention, but scope of protection of the present invention is not limited thereto, it is any Those familiar with the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its invents Design is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.

Claims

1. a kind of device for extracting stevioside from STEVIA REBAUDIANA, including feed pipe (1), it is characterised in that: the feed pipe (1) side is equipped with the first feeding device, and first feeding device is equipped with crush box (14), sets on the crush box (14) There is grinding device, the lower end side of the crush box (14), which is run through, is equipped with the second conveying pipeline (28), second conveying pipeline (28) It is equipped with filter device, the filter device is equipped with shell (20), and mixing arrangement, the mixing are equipped in the shell (20) The side of device is equipped with primary heater (29), and the side of the primary heater (29) is connected with ultrasonic generator (30), institute The side for stating ultrasonic generator (30) is connected with the first holding vessel (31) and the second holding vessel (32), second holding vessel (32) Side be connected with third conveying pipeline (35), the side of the third conveying pipeline (35) is equipped with water bath heating device, the water-bath The side of heating device is connected with centrifuge (41), and the side of the centrifuge (41) is connected with the first blending tank (42), described The side of first blending tank (42) is equipped with fixing pipe, and the side of fixing pipe is connected to the side of the first holding vessel (31), described First blending tank (42) is connected with absorber (48), and the side of the absorber (48) is connected with elution tank (49), described to wash The side of de- tank (49) is connected with silica gel column chromatography device (50), and the side of the silica gel column chromatography device (50) is connected with the second mixing The side of tank (51), second blending tank (51) is connected with Centrifugal concentrators (52), second blending tank (51) it is another Side is connected with NaOH solution tank NaOH (53), and the side of the Centrifugal concentrators (52) is connected with drier (54).

2. a kind of device for extracting stevioside from STEVIA REBAUDIANA according to claim 1, which is characterized in that described the One feeding device includes cleaning case (2) of the setting in feed pipe (1) lower end, and the lower end of feed pipe (1) is run through and extended to clear In tank-washer (2), the side of the cleaning case (2) is equipped with water pump (47), and the side of water pump (47) is connected with the first water inlet pipe (7), and the lower end of the first water inlet pipe (7) is run through and is used and extends in cleaning case (2), and the other side of the cleaning case (2) is run through Equipped with the first conveying pipeline (5), and the first water inlet pipe (7) is located between feed pipe (1) and the first conveying pipeline (5), and described first is defeated The upper end of expects pipe (5) is fixed with the first driving motor (8), and the output shaft of first driving motor (8) runs through the first conveying pipeline (5) it and extends in the first conveying pipeline (5), the output shaft end of the first driving motor (8) is fixed with the first spiral conveying bar (6), and the lower end of the first spiral conveying bar (6) successively wears the first conveying pipeline (5), cleaning case (2) and is rotatably connected on cleaning case (2) bottom in, the upper end side of first conveying pipeline (5) through setting in the upper end side of crush box (14), it is described clear The lower end of tank-washer (2) is equipped with drainpipe (3).

3. a kind of device for extracting stevioside from STEVIA REBAUDIANA according to claim 2, which is characterized in that the powder Crushing device includes the second driving motor (9) for being fixed on crush box (14) upper end, and the output shaft of second driving motor (9) passes through It wears crush box (14) and extends in crush box (14), the output shaft end of second driving motor (9) is fixed with the second spiral shell It revolves conveying bar (13), and the lower end of the second spiral conveying bar (13) through crush box (14) interior bottom and extends to crush box (14) Lower end, the lower end of the second spiral conveying bar (13) is fixed with first gear (17), the bottom in the crush box (14) Rotation is socketed with two rotating bars (16), and the second spiral conveying bar (13) is located between two rotating bars (16), two rotations The lower end of bar (16) is respectively and fixedly provided with second gear (18), and two second gears (18) are meshed with first gear (17), and two The upper end of rotating bar (16) is equidistantly fixed with multiple crushing knifes (15), the other side of the water pump (47) be connected with second into The lower end of water pipe (10), second water inlet pipe (10) is run through the upper end of crush box (14) and is extended in crush box (14), institute The lower end for stating the second water inlet pipe (10) is fixed with connecting tube (11), and the upper end of connecting tube (11) is fixed on top in crush box (14) The lower end in portion, the connecting tube (11) is equidistantly equipped with multiple spray heads (12), and second driving motor (9) is located at the first conveying It manages between (5) and the second water inlet pipe (10).

4. a kind of device for extracting stevioside from STEVIA REBAUDIANA according to claim 1, which is characterized in that the mistake Filtering device includes the 5th driving motor (46), is equipped with gap (24), institute in the opposing sidewalls in second conveying pipeline (28) It states and is mounted on link block (25) in gap (24), be fixed with filter plate (26), the company jointly between two link blocks (25) The lower end side for connecing block (25) is rotatablely connected there are two the polished rod (43) being parallel to each other, and the lower end of the polished rod (43) is run through It equipped with casing (44), is arranged with spring (45) on the polished rod (43), and spring (45) is located in casing (44), the spring (45) upper end is fixed on the top in casing (44), and the lower end of the spring (45) is fixed on the lower end of polished rod (43), described 5th driving motor (46) is mounted on the side of the second conveying pipeline (28), and the output shaft of the 5th driving motor (46) is through the Two conveying pipelines (28) simultaneously extend in the second conveying pipeline (28), and the output shaft end bias of the 5th driving motor (46) is fixed Have rotor plate (27), and the upper end of rotor plate (27) and the lower end of filter plate (26) are inconsistent, second conveying pipeline (28) Lower end is through setting in the upper end side of shell (20).

5. a kind of device for extracting stevioside from STEVIA REBAUDIANA according to claim 1, which is characterized in that described mixed It attaches together and sets the third driving motor (23) including being mounted on shell (20) side, the output shaft of third driving motor (23) runs through shell The side of body (20) simultaneously extends in shell (20), and the output shaft end of the third driving motor (23) is fixed with the first stirring Axis (21), one end of first agitating shaft (21) are rotatably connected on one end side wall in shell (20), first stirring Multiple first paddles (22) are equidistantly equipped on axis (21), the upper end other side of the shell (20) is fixed with cellulase storage It deposits case (19), the lower end of the cellulase storage bin (19) is run through and extended in shell (20).

6. a kind of device for extracting stevioside from STEVIA REBAUDIANA according to claim 1, which is characterized in that the water Bathing heating device includes the heating tank (55) through setting in third conveying pipeline (35) one end, is equipped with and adds in the heating tank (55) Hot chamber, the heating tank (55) is interior to be equipped with mixing chamber, and mixing chamber is located in heating chamber, and the lower end of the heating tank (55) is equipped with The lower end quadrangle of secondary heater (38), the heating tank (55) is respectively and fixedly provided with second support (37), and secondary heater (38) Between four second supports (37), the upper end of the heating tank (55) is fixed with the 4th driving motor (34), and the described 4th The output shaft end of driving motor (34) is through heating tank (55) and extends in mixing chamber, the 4th driving motor (34) Output shaft end is fixed with the second agitating shaft (40), and the lower end of the second agitating shaft (40) is rotatably connected on the bottom in stir chamber Portion is equidistantly equipped with multiple second paddles (39), the upper end side of the heating tank (55) on second agitating shaft (40) It is fixed with ether holding vessel (33), the lower end of the ether holding vessel (33) through heating tank (55) and extends in mixing chamber.

7. a kind of device for extracting stevioside from STEVIA REBAUDIANA according to claim 4, which is characterized in that the set Managing (44) is cylinder closed at one end, and described sleeve pipe (44) is closed end close to the one end of link block (25).

8. a kind of device for extracting stevioside from STEVIA REBAUDIANA according to claim 2, which is characterized in that described clear The side of tank-washer (2) is fixed on the lower end side of crush box (14), and the lower end of the cleaning case (2) and crush box (14) is jointly solid Surely have first support (4), and shell (20) is located in first support (4).