CN208537470U

CN208537470U - Binary channel glucose oxidase film electrode

Info

Publication number: CN208537470U
Application number: CN201721493498.5U
Authority: CN
Inventors: 刘峻
Original assignee: Shanghai Hileap Medical Technology Co Ltd
Current assignee: Shanghai Hileap Medical Technology Co Ltd
Priority date: 2017-11-10
Filing date: 2017-11-10
Publication date: 2019-02-22
Anticipated expiration: 2027-11-10

Abstract

The utility model provides a kind of binary channel glucose oxidase film electrode, die substrate, and the auxiliary signal contact formed on the die substrate, detect the-the first output contact of signal, detect the-the second output contact of signal, auxiliary electrode, detecting electrode the-the first auxiliary signal contact and the-the second auxiliary signal of detecting electrode contact, wherein, it further include a plurality of conducting wire, the auxiliary electrode, detecting electrode the-the first auxiliary signal contact and detecting electrode the-the second auxiliary signal contact by a plurality of conducting wire respectively with the auxiliary signal contact, detect the-the first output contact of signal and detection the-the second output contact of signal electrical connection.The double detection designs of binary channel provided by the utility model, more ensure that the accuracy of detection numerical value.

Description

Binary channel glucose oxidase film electrode

Technical field

The utility model relates to biological fields.

Background technique

With the promotion of bioscience, information science and material science development result, biosensor technology rapid development. But currently, the extensive use of biosensor is still faced with some difficulties, following period of time, biosensor are ground from now on Studying carefully work will be mainly around the biological sensing element that selection activity is strong, selectivity is high；Improve the service life of signal detector；It mentions The service life of high RST converter；The micromation of the stability of biological response and biosensor, it is portable the problems such as.It can be with , it is envisioned that following biosensor will have the characteristics that.

Following biosensor will be further to health care, medical diagnosis on disease, food inspection, environmental monitoring, fermentation The every field of industry.One of important content in biosensor research is exactly that research can replace biological vision, smell, taste The sensory biosensors such as feel, the sense of hearing and tactile, here it is biomimetic sensors, also referred to as using biosystem as model Biosensor.

It is biological component (enzyme, antigen, antibody, hormone, DNA) or organism itself (cell, organelle, tissue), it Can specifically identify various measured matters and react；The latter mainly has electrochemical electrode, ion sensing fet (ISFET), thermal resistor, photoelectric tube, optical fiber, piezo-electric crystal (PZ) etc., function are the bioid for perceiving sensing element It learns signal and is changed into measurable electric signal.

Biosensor presses the difference of molecular recognition elements used, can be divided into enzyme sensor, microbiological sensor, tissue and pass Sensor, cell organelle sensor, immunosensor etc.；By the difference of signal conversion element, electrochemica biological sensor, half can be divided into Conductor biosensor, heat-measuring type biosensor, survey light type biosensor, survey sound type biosensor etc.；By to output The different measurement methods of electric signal, and electric potential type biosensor, amperometric biosensor and volt-ampere type biology can be divided into and passed Sensor.Microbiological sensor is an important branch of biosensor.Divies in 1975 has been made first microorganism and has passed Thus sensor opens the another frontier of biosensor development.

When not damaging microorganism function, microorganism can be fixed on carrier and produce microbiological sensor.It is micro- For biosensor compared with enzyme sensor, it has following characteristics:

(1) the bacterial strain score of microorganism is much lower from the price of enzyme purification, thus manufactured sensor is convenient for popularizing；

(2) activity is not easy to reduce the enzyme in microbial cell under appropriate circumstances, therefore the service life of microbiological sensor is more It is long；

Even if (3) the catalytic activity of the intracorporal enzyme of microorganism has been lost, can also be allowed to regenerate because of the proliferation of cell；

(4), for needing the complicated successive reaction of confactor, then it is easier to complete with microorganism.

But biosensor of the existing technology has that Detection accuracy is low.

Utility model content

The utility model provides a kind of binary channel glucose oxidase film electrode, die substrate, and in the chip The auxiliary signal contact that is formed on bottom plate, detection the-the first output contact of signal, detection the-the second output contact of signal, auxiliary electricity Pole, detecting electrode the-the first auxiliary signal contact and the-the second auxiliary signal of detecting electrode contact, wherein further include a plurality of conductor wire Road, the auxiliary electrode, detecting electrode the-the first auxiliary signal contact and detecting electrode the-the second auxiliary signal contact pass through described A plurality of conducting wire is touched with the auxiliary signal contact, detection the-the first output contact of signal and the detection output of signal-the second respectively Point electrical connection.

Preferably, the auxiliary electrode and the-the first auxiliary signal of detecting electrode contact, the-the second auxiliary signal of detecting electrode Contact electrode top is covered with the glucose oxidase for having bioactivity with protection permeable membrane.

Preferably, the auxiliary signal contact, detection the-the first output contact of signal, detection the-the second output contact of signal with Auxiliary electrode, the-the first auxiliary signal of detecting electrode contact, the line surface among the-the second auxiliary signal of detecting electrode contact are equal Insulating protective film is covered, to prevent electric signal from losing or decaying.

Preferably, the auxiliary signal contact, detection the-the first output contact of signal, detection the-the second output contact of signal are Metal material contact.

Preferably, the die substrate is the substrate of ceramic material, and the die substrate is the substrate of ceramic material, is passed through Etching method, which is processed, to be formed the auxiliary signal contact, detection the-the first output contact of signal, detect the-the second output contact of signal, is auxiliary Help electrode, the-the first auxiliary signal of detecting electrode contact, detecting electrode the-the second auxiliary signal contact and a plurality of conducting wire.

The double detection designs of binary channel provided by the utility model, more ensure that the accuracy of detection numerical value.

Detailed description of the invention

The structural schematic diagram of Fig. 1 an embodiment of the present invention；

Appended drawing reference:

1, die substrate

2, auxiliary signal contact

3, the-the first output contact of signal is detected

4, the-the second output contact of signal is detected

5, auxiliary electrode

6, the-the first auxiliary signal of detecting electrode contact

7, the-the second auxiliary signal of detecting electrode contact

Specific embodiment

Further illustrate the technical solution of the utility model below with reference to the accompanying drawings and specific embodiments.It can manage Solution, specific embodiment described herein are only used for explaining the utility model, rather than the restriction to the utility model.In addition It should also be noted that, illustrating only part relevant to the utility model for ease of description, in attached drawing rather than full content.

In conjunction with Fig. 1, die substrate 1 is the substrate of ceramic material, processes die substrate 1, auxiliary using etching method on it Help signalling contact 2, detection the-the first output contact of signal 3, auxiliary electrode 5,6 component of the-the first auxiliary signal of detecting electrode contact with And connection line.Then in auxiliary electrode 5 and the-the first auxiliary signal of detecting electrode contact 6, the-the second auxiliary signal of detecting electrode 7 electrode upper of contact is covered with the glucose oxidase for having bioactivity with protection permeable membrane, auxiliary signal contact 2, inspection The-the first output contact of signal 3, detection the-the second output contact of signal 4 are surveyed as metal material contact, passes through the metal being in contact with it Electric signal obtained is transferred in the analysis circuit of instrument by component.Auxiliary signal contact 2, the detection output touching of signal-the first Point 3, detection the-the second output contact of signal 4 and auxiliary electrode 5, the-the first auxiliary signal of detecting electrode contact 6, detecting electrode-the Line surface among two auxiliary signal contact 7 covers insulating protective film, to prevent electric signal from losing or decaying.

When work, inspected blood sample or the sample droplets after diluting are assisted in auxiliary electrode 5, detecting electrode-the first What signalling contact 6,7 electrode upper of the-the second auxiliary signal of detecting electrode contact were covered has biological work with protection permeable membrane Property glucose oxidase at, contained glucose (C in examined samples₆H₁₂O₆) macromolecular is through permeable membrane, with internal Portugal Grape carbohydrate oxidase (GOD) reacts, and is generated gluconic acid (C after glucose oxidase (GOD) oxidation₆H₁₂O₇) and peroxide Change hydrogen (H₂O₂) two kinds of compositions, the variation of composition will affect its conductivity, and according to both compositions caused by reacting , auxiliary electrode 5 and detecting electrode-the first auxiliary signal contact 6 electrode different containing numerical quantity, auxiliary electrode 5 and detecting electrode- The electron number exchanged between second auxiliary signal contact, 7 electrode also will be different, will generate different electric signals.Detecting electrode- First auxiliary signal contact 6, the-the second auxiliary signal of detecting electrode contact 7 are led to detected different electrical signals by conducting wire Cross detection the-the first output contact of signal 3, detection 4 contact of the-the second output contact of signal is transferred to the analysis circuit of instrument internal In, after calculating, can be obtained the glucose in examined samples contains numerical quantity.

By the double detection designs of binary channel, the accuracy of detection numerical value more ensure that.

The above is only the preferred embodiments of the utility model, are not intended to limit the utility model, all in the utility model Spirit and principle within, any modification, equivalent replacement, improvement and so on should be included in the protection model of the utility model Within enclosing.

Claims

1. a kind of binary channel glucose oxidase film electrode, it is characterised in that: die substrate, and the shape on the die substrate At auxiliary signal contact, detection the-the first output contact of signal, detection the-the second output contact of signal, auxiliary electrode, detection electricity Pole the-the first auxiliary signal contact and the-the second auxiliary signal of detecting electrode contact, wherein it further include a plurality of conducting wire, it is described auxiliary Electrode, detecting electrode the-the first auxiliary signal contact and detecting electrode the-the second auxiliary signal contact is helped to pass through a plurality of conductor wire Road is electrically connected with the auxiliary signal contact, detection the-the first output contact of signal and detection the-the second output contact of signal respectively, The auxiliary electrode and the-the first auxiliary signal of detecting electrode contact, detecting electrode the-the second auxiliary signal contact electrode top cover There is the glucose oxidase for having bioactivity with protection permeable membrane.

2. binary channel glucose oxidase film electrode as described in claim 1, it is characterised in that: the auxiliary signal contact, Detect the-the first output contact of signal, detection the-the second output contact of signal and auxiliary electrode, the-the first auxiliary signal of detecting electrode is touched Line surface among point, the-the second auxiliary signal of detecting electrode contact covers insulating protective film, with prevent electric signal lose or Decaying.

3. binary channel glucose oxidase film electrode as described in claim 1, it is characterised in that: the auxiliary signal contact, Detect the-the first output contact of signal, detection the-the second output contact of signal is metal material contact.

4. binary channel glucose oxidase film electrode as described in claim 1, it is characterised in that: the die substrate is ceramics The substrate of material is processed to form the auxiliary signal contact, detection the-the first output contact of signal, detection signal-by etching method Second output contact, auxiliary electrode, the-the first auxiliary signal of detecting electrode contact, detecting electrode the-the second auxiliary signal contact and more Conducting wire.