CN208500958U - A kind of sperm preferred embodiment - Google Patents
A kind of sperm preferred embodiment Download PDFInfo
- Publication number
- CN208500958U CN208500958U CN201820480664.6U CN201820480664U CN208500958U CN 208500958 U CN208500958 U CN 208500958U CN 201820480664 U CN201820480664 U CN 201820480664U CN 208500958 U CN208500958 U CN 208500958U
- Authority
- CN
- China
- Prior art keywords
- helical duct
- sperm
- tube body
- centrifuge tube
- pipe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 230000032798 delamination Effects 0.000 claims abstract description 12
- 239000012530 fluid Substances 0.000 claims abstract description 12
- 238000005119 centrifugation Methods 0.000 claims description 19
- 238000000034 method Methods 0.000 abstract description 15
- 238000000432 density-gradient centrifugation Methods 0.000 abstract description 5
- 230000005012 migration Effects 0.000 abstract description 3
- 238000013508 migration Methods 0.000 abstract description 3
- 239000007788 liquid Substances 0.000 description 10
- 239000008188 pellet Substances 0.000 description 5
- 239000006228 supernatant Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 239000012634 fragment Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 230000005659 seminal clot liquefaction Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000010902 straw Substances 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000009027 insemination Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 229910000077 silane Inorganic materials 0.000 description 1
- 230000019100 sperm motility Effects 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
Abstract
The utility model relates to a kind of sperm preferred embodiments, including centrifuge tube lid, it is centrifuged tube body and is mounted on the intracorporal helical duct of centrifuge tube, being centrifuged tube body is made of tube body and bottom pyramidal section, bottom of the pipe is connected through a screw thread with bottom pyramidal section, helical duct is divided into helical duct and lower helical duct, it is spaced 5-15mm between upper helical duct bottom end and lower helical duct upper end and forms centrifuge tube delamination area, upper helical duct and lower helical duct are connected to centrifuge tube delamination area respectively, lower helical duct is connected to by vertical fluid stopping pipe with bottom pyramidal section, the diameter of vertical fluid stopping pipe is 3-6mm, length is 10-20mm.The characteristics of the utility model extends the path of sperm migration in density gradient using the principle of helix tube, while utilization motile sperm actively moves about, the efficiency that discontinuous density gradient centrifugation method separates high-quality sperm can be improved；The utility model can also shorten experimental period, conducive to the standardization of operation.
Description
Technical field
The utility model relates to a kind of sperm preferred embodiments.
Background technique
Need to separate sperm from refining in many cases, for sperm function detection, it is therapeutic insemination and
Assisted reproductive technology (ART) etc..Common spermatozoa isolation optimization technique has method immediately upstream and discontinuous density gradient method.
Discontinuous density gradient centrifugation method can isolate good sperm, divide especially from other cell components and fragment
Separate out good sperm.It is easier to standardize than method immediately upstream, as a result also more stable.The sperm of this method preparation and recycling
It is usually used in IVF and ICSI.
Discontinuous density gradient centrifugation method is that sperm is placed on to the density being made of the colloid preparation that surface is coated with silane
In gradient media, sperm is then obtained by centrifuge separation according to the of different sizes of cell density.In addition, motile sperm can be worn
It crosses density material and swims over to test tube bottom.It is density gradient that simple two different density mediums, which form discontinuous gradient preparation method,
The most common method of method, classical way are placed on upper layer using 40% density medium, and 80% is placed on bottom.Discontinuous density gradient from
Heart method can obtain the sperm of higher vigor, and without fragment, leucocyte, bacterium.
But in practical operation, since the factors such as sperm is sticky, Necrospermia ratio is more or laboratory technician are with suction pipe
A large amount of supernatant misoperations of Sperm pellets group top after being centrifuged are drawn, the sperm motility of part semen sample final separation is not
It is that very well, can also be mingled with a little spermatin particle or some Necrospermias once in a while.
Summary of the invention
Technical problem to be solved by the utility model is: providing a kind of sperm preferred embodiment, the utility model uses spiral
The characteristics of principle of pipe extends the path of sperm migration in density gradient, while utilization motile sperm actively moves about,
The efficiency that discontinuous density gradient centrifugation method separates high-quality sperm can be improved, it is preferred in addition to carry out sperm using the utility model
Operation can shorten experimental period, while be conducive to the standardization of operation.
Solve above-mentioned technical problem technical solution be: a kind of sperm preferred embodiment, including centrifuge tube lid, centrifugation tube body and
Be mounted on the intracorporal helical duct of centrifuge tube, the centrifugation tube body is made of tube body and bottom pyramidal section, bottom of the pipe with
Bottom pyramidal section is connected through a screw thread, and the helical duct is divided into helical duct and lower helical duct, upper helical duct bottom end
Be spaced 5-15mm between lower helical duct upper end and form centrifuge tube delamination area, upper helical duct and lower helical duct respectively with
The connection of centrifuge tube delamination area, lower helical duct are connected to by vertical fluid stopping pipe with bottom pyramidal section, the diameter of vertical fluid stopping pipe
For 3-6mm, length 10-20mm.
The centrifugation tube body subscript of the centrifuge tube delamination area is marked with layering midpoint graduation mark.
The centrifuge tube lid is connected through a screw thread with centrifugation tube body upper end.
Due to the adoption of the above technical scheme, the utility model has the following beneficial effects:
1, the utility model extends the path of sperm migration in density gradient using the principle of helix tube, simultaneously
The characteristics of actively being moved about using motile sperm, under the effect of external centrifugal force, so that the good sperm of vigor is easier toward centrifuge tube
Lower part is walked, and the efficiency that discontinuous density gradient centrifugation method separates high-quality sperm can be improved.
2, the utility model bottom of the pipe and bottom pyramidal section are connected through a screw thread, after centrifugation, then by bottom pyramidal
Section, which removes, separates it with tube body.For Sperm pellets cumularsharolith in bottom pyramidal section, Sperm pellets group top is only a small amount of after centrifugation
Supernatant within the allowable range, laboratory technician is not required to draw the supernatant of Sperm pellets group top with suction pipe, to avoid drawing
The case where being mixed into spermatin particle or some Necrospermias is easy when supernatant misoperation, benefit while shortening experimental period
In the standardization of operation.
In the following, making in conjunction with the accompanying drawings and embodiments to a kind of technical characteristic of sperm preferred embodiment of the utility model further
Explanation.
Detailed description of the invention
Fig. 1: the sperm preferred embodiment structural schematic diagram (office is cutd open) of the utility model.
Fig. 2: the sperm preferred embodiment axonometric drawing of the utility model.
Fig. 3: the sperm preferred embodiment usage state diagram (before centrifugally operated) of the utility model.
Fig. 4: the sperm preferred embodiment usage state diagram (after centrifugally operated) of the utility model.
In figure: 1- centrifuge tube lid, 2- are centrifuged tube body, 21- tube body, 22- bottom pyramidal section, 3- helical duct, the upper spiral of 31-
Channel, helical duct under 32-, 4- centrifuge tube delamination area, 41- are layered midpoint graduation mark, the vertical fluid stopping pipe of 5-.
P1 indicates that upper layer separating liquid ISolate 50%, P2 indicate that lower layer's separating liquid ISolate 90%, P3 indicate that sperm is heavy
Shallow lake group.
Specific embodiment
Embodiment 1: a kind of sperm preferred embodiment, as Figure 1-Figure 2, including centrifuge tube lid 1, centrifugation tube body 2 and installation
In the intracorporal helical duct 3 of centrifuge tube, the centrifugation tube body is made of tube body 21 and bottom pyramidal section 22, bottom of the pipe with
Bottom pyramidal section is connected through a screw thread, and the helical duct 3 divides for upper helical duct 31 and lower helical duct 32, upper helical duct
It is spaced 5-15mm between bottom end and lower helical duct upper end and forms centrifuge tube delamination area 4, upper helical duct and lower helical duct
It is connected to respectively with centrifuge tube delamination area, lower helical duct is connected to by vertical fluid stopping pipe 5 with bottom pyramidal section, vertical fluid stopping pipe
It is required that caliber is smaller, length is longer, and the diameter of general vertical fluid stopping pipe is 3-6mm, and vertical fluid stopping is arranged in length 10-20mm
The purpose of pipe is to guarantee to tear bottom pyramidal section 22 from tube body 21 open in the case where centrifuge tube lid 1 covers tightly centrifugation tube body 2
After unloading down, liquid will not be flowed out from vertical fluid stopping pipe.
In the present embodiment, the centrifugation tube body subscript of the centrifuge tube delamination area 4 is marked with layering midpoint graduation mark 41, in this way
Convenient for operation.As a kind of transformation, layering midpoint graduation mark can also be not provided with.
In the present embodiment, the centrifuge tube lid is connected through a screw thread with centrifugation tube body upper end.As a kind of transformation, centrifuge tube
Lid can also use the connection type of other easy disassembly with centrifugation tube body.
The sperm preferred method carried out using the utility model, as Figure 3-Figure 4, using discontinuous density gradient method,
The following steps are included:
(1) all liq is allowed to be warmed to room temperature or 37 DEG C；
(2) the centrifuge tube lid of sperm preferred embodiment is unscrewed；
Use disposable sterilized suction pipe, draw 2ml lower layer separating liquid ISolate 90%, be added sperm preferred embodiment from
The nozzle of heart tube body, lower layer's separating liquid after addition flow to the bottom of centrifugation tube body by helical duct, lower layer's separating liquid it is upper
End liquid level is overlapped with layering midpoint graduation mark；
Use a new aseptic straw, add the upper layer 2ml separating liquid ISolate 50%, make upper layer separating liquid with
Apparent layering is formed between lower layer's separating liquid；
(5) semen liquefaction is sufficiently mixed with 5ml or 10ml volume pipet；
(6) new aseptic straw is used, 1.5 ~ 2.0ml semen liquefaction is added in the centrifuge tube body of sperm preferred embodiment；
(7) the centrifuge tube lid for tightening sperm preferred embodiment is centrifuged 15-30 minutes under the centrifugal force of 300-400g；
(8) the bottom pyramidal section that sperm preferred embodiment centrifugation tube body is gently unscrewed after being centrifuged, separates it with upper portion pipe body,
The Sperm pellets group of high-quality vigor can be obtained.
Claims (3)
1. a kind of sperm preferred embodiment, it is characterised in that: including centrifuge tube lid (1), centrifugation tube body (2) and be mounted on centrifugation tube body
Interior helical duct (3), the centrifugation tube body are made of tube body (21) and bottom pyramidal section (22), bottom of the pipe and bottom
Conical section is connected through a screw thread, and the helical duct (3) is divided into helical duct (31) and lower helical duct (32), and upper spiral is logical
It is spaced 5-15mm between road bottom end and lower helical duct upper end and forms centrifuge tube delamination area (4), upper helical duct and lower spiral
Channel is connected to centrifuge tube delamination area respectively, and lower helical duct is connected to by vertical fluid stopping pipe (5) with bottom pyramidal section, vertically
The diameter of fluid stopping pipe is 3-6mm, length 10-20mm.
2. a kind of sperm preferred embodiment according to claim 1, it is characterised in that: the centrifuge tube delamination area (4)
Centrifugation tube body subscript is marked with layering midpoint graduation mark (41).
3. a kind of sperm preferred embodiment according to claim 1, it is characterised in that: on the centrifuge tube lid and centrifugation tube body
End is connected through a screw thread.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820480664.6U CN208500958U (en) | 2018-04-04 | 2018-04-04 | A kind of sperm preferred embodiment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820480664.6U CN208500958U (en) | 2018-04-04 | 2018-04-04 | A kind of sperm preferred embodiment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208500958U true CN208500958U (en) | 2019-02-15 |
Family
ID=65295748
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820480664.6U Expired - Fee Related CN208500958U (en) | 2018-04-04 | 2018-04-04 | A kind of sperm preferred embodiment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208500958U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108485935A (en) * | 2018-04-04 | 2018-09-04 | 柳州市妇幼保健院 | A kind of sperm preferred embodiment and method |
-
2018
- 2018-04-04 CN CN201820480664.6U patent/CN208500958U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108485935A (en) * | 2018-04-04 | 2018-09-04 | 柳州市妇幼保健院 | A kind of sperm preferred embodiment and method |
| CN108485935B (en) * | 2018-04-04 | 2023-07-04 | 柳州市妇幼保健院 | Sperm optimizing device and method |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190215 |
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| CF01 | Termination of patent right due to non-payment of annual fee |