CN208420822U - Titer determination device and virus infectivity detection device - Google Patents
Titer determination device and virus infectivity detection device Download PDFInfo
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- CN208420822U CN208420822U CN201820241602.XU CN201820241602U CN208420822U CN 208420822 U CN208420822 U CN 208420822U CN 201820241602 U CN201820241602 U CN 201820241602U CN 208420822 U CN208420822 U CN 208420822U
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- titer determination
- determination device
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Abstract
The utility model provides a kind of titer determination device and virus infectivity detection device, is related to biochemical test Instrument technology field, the titer determination device, comprising: detection plate and dilution disk, the detection plate are detachably connected with the dilution disk；Detection hole is provided in the detection plate, the detection hole is for carrying out biochemical reaction；It is provided on the dilution disk and contains liquid portion and dilution part, the Sheng liquid portion is used to carry out the dilution of determinand for holding dilution, the dilution part.Two device combinations link together, on the one hand, more convenient when use, the time before saving experiment；On the other hand, laboratory technician only needs to carry out the operation such as imbibition, dilution and mixing on the apparatus, and requires no the operation of the stations such as liquid feeding in centrifuge tube, centrifuge tube dilution and oscillator mixing.It is busy between multiple stations that the utility model device avoids experimental implementation, keeps experimentation more simple.
Description
Technical field
The utility model relates to biochemical test Instrument technology fields, feel more particularly, to a kind of titer determination device and virus
Contaminate power detection device.
Background technique
Virus is that one kind does not have eucaryotic cell structure, has the microorganism of the vital signs such as heredity, duplication.Generally spherical disease
Poison, baculoviral and bullet-shaped virus.Virus is the same with all biologies, has the ability of heredity, variation, evolution, is one
Kind volume is very small, the extremely simple life form of structure.Virus has the parasitics of height, is completely dependent on the energy of host cell
Amount and metabolic system, matter and energy needed for obtaining vital movement, leave host cell.It is a big chemical molecular,
Stop action, can be made into crystallization of protein, be a non-life body, encounter host cell it can by absorption, entrance, duplication,
Assembly discharges progeny virus and shows typical life body characteristics, so virus is original with abiotic one kind between biology
Life entity.
It is well known that the measurement of viral level is the important and cumbersome repeatability behaviour carried out in virus research work
Make.Routine operation is that virus is carried out to 10 times of doubling dilutions in centrifuge tube, and the venom of each dilution is then added to 96 holes
Corresponding each column in plate.Before carrying out above-mentioned experiment, it is necessary first to carry out laboratory apparatus, experiment reagent, test consumptive material
Prepare；Secondly it needs that viral dilution mistake could be completed by the operation of the instrument and equipments such as liquid feeding, oscillator mixing in centrifuge tube
Journey；Finally the venom diluted in centrifuge tube is added sequentially in the corresponding every hole of 96 orifice plates.Normal operation is complicated, out
Error rate significantly improves；Furthermore sometimes because the missing of part instrument can also delay the schedule entirely tested.
Utility model content
The purpose of this utility model is to provide a kind of titer determination device and virus infectivity detection devices, existing to improve
There are complex operations existing for experimental implementation, alleviate the existing laboratory apparatus in relation to needing to prepare largely to disperse in biochemical test,
The cumbersome technical problem of preliminary preparation is solved, convenient for the smooth development of experiment.
In a first aspect, a kind of titer determination device provided by the embodiment of the utility model, comprising: detection plate and dilution disk,
The detection plate is detachably connected with the dilution disk；
Detection hole is provided in the detection plate, the detection hole is for carrying out biochemical reaction；
It is provided on the dilution disk and contains liquid portion and dilution part, the Sheng liquid portion is for holding dilution, the dilution part
For carrying out the dilution of determinand.
With reference to first aspect, the utility model embodiment provides the first possible embodiment of first aspect,
In, the quantity of the detection hole is multiple.
With reference to first aspect, the utility model embodiment provides second of possible embodiment of first aspect,
In, along first direction, multiple detection holes are arranged along linear interval, form gradient detection hole group.
The possible embodiment of second with reference to first aspect, the utility model embodiment provide the of first aspect
Three kinds of possible embodiments, wherein the quantity of the gradient detection hole group is multiple groups, and gradient detection hole group edge described in multiple groups
Second direction arrangement, the first direction are vertical with second direction.
The third possible embodiment with reference to first aspect, the utility model embodiment provide the of first aspect
Four kinds of possible embodiments, wherein the bottom of the detection hole is flat.
The 4th kind of possible embodiment with reference to first aspect, the utility model embodiment provide the of first aspect
Five kinds of possible embodiments, wherein the dilution part includes dilution holes, the quantity of the dilution holes and the gradient detection hole
The quantity of detection hole in group is identical, and corresponds；Alternatively,
The dilution part includes dilution trap, the number of the detection hole in the quantity of the dilution trap and the gradient detection hole group
Measure it is identical, and correspond.
The 5th kind of possible embodiment with reference to first aspect, the utility model embodiment provide the of first aspect
Six kinds of possible embodiments, wherein along the depth direction of the dilution holes, the cross-sectional area of the dilution holes first increases and subtracts after
It is small.
With reference to first aspect, the utility model embodiment provides the 7th kind of possible embodiment of first aspect,
In, the titer determination device includes detection lid, and the detection lid is for buckle closure in the detection plate and dilution disk.
The 7th kind of possible embodiment with reference to first aspect, the utility model embodiment provide the of first aspect
Eight kinds of possible embodiments, wherein it is provided with recess portion in the detection plate, is provided with protrusion on the dilution disk, it is described recessed
Portion and convex combination, for the detection plate and the dilution disk to be clamped.
Second aspect, a kind of virus infectivity detection device provided by the embodiment of the utility model, including above-mentioned titre
Measurement device.
The utility model embodiment the utility model has the advantages that
A kind of titer determination device and virus infectivity detection device provided by the embodiment of the utility model, the titre are surveyed
Determining device includes: detection plate and dilution disk, uses the connection type being detachably connected between the detection plate and dilution disk, in order to
Enough facilitate separate operations, storage, use and the transport of detection plate and dilution disk after operating.Detection is provided in detection plate
Hole, detection hole are exactly the reacting environment of biochemical reaction experiment.It is provided on the dilution disk and contains liquid portion and dilution part, the Sheng liquid
Portion is used to carry out the dilution of determinand for holding dilution, the dilution part.In carrying out biochemical test, need to determinand
It is diluted, and conventional means are to be placed into determinand in centrifuge tube to be diluted, and then pass through oscillator, can be added to
In detection device, during Preparatory work of experiment, it often will appear experiment equipment and prepare insufficient problem, and use the utility model
The titer determination device that embodiment provides is tested, so that it may save the preparation of many experiments instrument in the process.
The dilution and detection to determinand can be completed using titer determination device provided by the embodiment of the utility model.Because two
Device links together, on the one hand, more convenient searching when use, the time before saving experiment；On the other hand, it tests
Member only needs to be diluted the experimental implementation with detection on the apparatus, and does not have to first carry out in the station of centrifuge tube and oscillator
Dilution operation, then detection operation is carried out in detection zone, it is busy between multiple stations, keep experimentation more simple.
Other feature and advantage of the utility model will illustrate in the following description, also, partly from specification
In become apparent, or understood and implementing the utility model.The purpose of this utility model and other advantages are illustrating
Specifically noted structure is achieved and obtained in book, claims and attached drawing.
To enable the above objects, features, and advantages of the utility model to be clearer and more comprehensible, preferred embodiment is cited below particularly, and
Cooperate appended attached drawing, is described in detail below.
Detailed description of the invention
It, below will be right in order to illustrate more clearly of specific embodiment of the present invention or technical solution in the prior art
Specific embodiment or attached drawing needed to be used in the description of the prior art are briefly described, it should be apparent that, it is described below
In attached drawing be that some embodiments of the utility model are not paying creativeness for those of ordinary skill in the art
Under the premise of labour, it is also possible to obtain other drawings based on these drawings.
Fig. 1 is a kind of top view of titer determination device provided by the embodiment of the utility model；
Fig. 2 is a kind of sectional view of the positive apparent direction of titer determination device provided by the embodiment of the utility model；
Fig. 3 is the top view of another titer determination device provided by the embodiment of the utility model；
Fig. 4 is the sectional view of the positive apparent direction of another titer determination device provided by the embodiment of the utility model；
Fig. 5 is the schematic diagram of the detection lid of titer determination device provided by the embodiment of the utility model.
Icon: 100- detection plate；110- detection hole；200- dilutes disk；210- contains liquid portion；220- dilution holes；230- dilution
Slot；300- detection lid.
Specific embodiment
The technical solution of the utility model is clearly and completely described below in conjunction with attached drawing, it is clear that described
Embodiment is the utility model a part of the embodiment, instead of all the embodiments.Based on the embodiments of the present invention, originally
Field those of ordinary skill every other embodiment obtained without making creative work belongs to practical
Novel protected range.
In the description of the present invention, it should be noted that such as occur term " center ", "upper", "lower", " left side ",
" right side ", "vertical", "horizontal", "inner", "outside" etc., the orientation or positional relationship of instruction are orientation based on the figure or position
Relationship is set, is merely for convenience of describing the present invention and simplifying the description, rather than the device or element of indication or suggestion meaning
It must have a particular orientation, be constructed and operated in a specific orientation, therefore should not be understood as limiting the present invention.This
Outside, such as there is term " first ", " second ", " third " are used for description purposes only, be not understood to indicate or imply relatively heavy
The property wanted.
As shown in Figs 1-4, a kind of titer determination device provided by the embodiment of the utility model, comprising: detection plate 100 and dilute
Disk 200 is released, using the connection type being detachably connected between the detection plate 100 and dilution disk 200, in order to facilitate detection plate 100
Storage with dilution disk 200 is used and is transported.Detection hole 110 is provided in detection plate 100, detection hole 110 is exactly biochemical anti-
The reacting environment that should be tested.It is provided on the dilution disk 200 and contains liquid portion 210 and dilution part, the Sheng liquid portion 210 is for holding
Dilution, the dilution part are used to carry out the dilution of determinand.In carrying out biochemical test, need to be diluted determinand,
And conventional means are to be placed into determinand in centrifuge tube to be diluted, and then pass through oscillator, can be added to detection device
On, during Preparatory work of experiment, it often will appear experiment equipment and prepare insufficient problem, and mentioned using the utility model embodiment
The titer determination device of confession is tested, so that it may save the preparation of many experiments instrument in the process.Use this reality
Dilution and detection to determinand can be completed with the titer determination device that new embodiment provides.Because of two device connections
Together, on the one hand, more convenient searching when use, the time before saving experiment；On the other hand, laboratory technician only needs
It is diluted the experimental implementation with detection on the apparatus, and does not have to first be diluted behaviour in the station of centrifuge tube and oscillator
Make, then carries out detection operation in detection zone, it is busy between multiple stations, keep experimentation more simple.
The device can carry out a variety of biochemical tests, for example, the experiment of virus infectivity titer determination, can also carry out enzyme
Linked immunosorbent adsorption test.
In the scheme that one can be implemented, as the basic structure of the device, detection hole 110 in detection plate 100
Quantity can be one, and the quantity of dilution holes 220 may be one, and the dilution to determinand is completed in dilution holes 220,
It may then pass through the auxiliary tools such as suction pipe the liquid after dilution is transferred in detection hole 110, this is basis and single knot
Structure.
In the scheme that one can be implemented, such as in the test of the measurement of virus infectivity, need virus to be measured
Carry out doubling dilution, the quantity of required detection hole 110 be it is multiple, the determinand for being diluted to different proportion is transferred to difference
Detection hole 110 in, can complete gradient reaction comparative experiments.
Specifically, multiple detection holes 110 are arranged along linear interval along first direction, gradient detection hole 110 is formed
Group.As shown in Figure 1, along its length, 110 groups of row's gradient detection hole can be set, and in first row gradient detection hole 110
Number designation, convenient observation and record to experimental phenomena has can be set in the top of group.For example, the concentration of required detection
Gradient number is 10, then, in the experimentation of virus infectivity titer determination, first the gradient that cell is placed on first row is examined
In the hole that 110 groups of gaging hole；Then in dilution zone, gradient dilution is carried out to determinand；It is then possible to which the gradient in first row detects
In the hole that 110 groups of hole, it is implanted sequentially the determinand of various concentration, concentration is respectively to take 10-1、10-2……10-10.It uses in this way
The device that the embodiment provides can carry out gradient dilution and detection, expand the scope of application of device.
Further, the quantity of 110 groups of the gradient detection hole can be multiple groups, and gradient detection hole 110 described in multiple groups
Group arranges in a second direction, and the first direction is vertical with second direction.Because only carrying out primary experiment is difficult have generality,
So common biochemical test is needed using multiplicating experiment.So it is provided with 110 groups of multiple rows of gradient detection hole, so as to
So that all detection holes 110 are arranged in arrays, and digital mark can be labeled with 110 groups of multiple rows of gradient detection hole of side
Number, Roman capitals label or continuous English alphabet label, a correspondence markings, the convenient record to experimental data are carried out to group number.
Preferably, the bottom of the detection hole 110 can be flat, for example, in the experiment of virus infectivity titer determination
In the process, the culture for needing to carry out cell in detection hole 110, will test 110 bottom of hole and is set as plane, it is ensured that cell is equal
Even culture.
Specifically, the dilution part may include dilution holes 220, the quantity and the gradient of the dilution holes 220 are detected
The quantity of detection hole 110 in 110 groups of hole is identical, and corresponds.For example, 110 groups of gradient detection hole include 10 detection holes
110, then the quantity of dilution holes 220 can be similarly 10, or it is more, gradient is completed in 10 dilution holes 220
Dilution operation, it is then possible to which dilution is added in detection hole 110 by the volley of rifle fire.
Along the depth direction of the dilution holes 220, the cross-sectional area of the dilution holes 220 first increases to be reduced after.Namely
It says, the opening of dilution holes 220 is relatively narrow, and middle section is wider, can be prevented in this way in dilution and dilution during taking liquid
Spluttering.
Multiple dilution holes 220 can be arranged in a linear, or annular array.
In the scheme that one can be implemented, dilution part may include dilution trap 230, and dilution holes 220 are can to use liquid relief
Object to be checked is carried out in each hole multiple proportions and is serially diluted by rifle, and diluted venom is then added to the corresponding detection of detection plate 100
In hole 110.Dilution trap 230 is object to be checked can be carried out multiple proportions with 8 liquid-transfering guns to be serially diluted in each slot, then will dilution
Venom be added in the corresponding detection hole 110 of detection plate 100.The dilution trap 230 of setting makes the speed of transfer liquid faster, behaviour
Make more convenient.
As shown in figure 5, the titer determination device can also include detection lid 300 in the scheme that can be implemented at one,
The material for detecting lid 300 can be transparent resin, and the detection lid 300 is for buckle closure in the detection plate 100 and dilution disk
On 200.In carrying out biochemical test, detection lid 300 can prevent extraneous condition interference, can mark on detection lid 300
Have with the lateral label and the corresponding label of vertical label in detection plate 100, in this way, when detection 300 buckle closure of lid is in detection plate
100, with after dilution disk 200, can also be observed that internal situation outside, then be recorded using label.
Recording areas can be set in the other side of detection lid 300, the recording areas can carry out sample ID, quantity, examination
Test the detailed record of the contents such as time
In the scheme that one can be implemented, it is provided with recess portion in the detection plate 100, is arranged on the dilution disk 200
There are protrusion, the recess portion and convex combination, for the detection plate 100 and the dilution disk 200 to be clamped.
For example, the recess portion is located on the side of the detection plate 100, the protrusion is located at the side of the dilution disk 200
Bian Shang docks the two, because material has certain elasticity, the two can be connected together.
In the scheme that another can be implemented, the marginal position of 100 lower surface of detection plate is provided with the first recess portion, described
The edge for diluting the upper surface of disk 200 is provided with the second recess portion, and detection plate 100 and the dilution up and down motion of disk 200 are connected to one
It rises, detection plate 100 and dilution disk 200 are placed on platform, and the two will not occur to move to direction away from each other.
The circle detection hole 110 of one specific embodiment, 8 × 10 arrangements is distributed in the upper surface of detection plate 100, small
Pore volume can be 300 μ L, and bottom is flat.Each column and every row have certain tactic Arabic numerals and English big respectively
Write mother.The Sheng liquid portion 210 for diluting disk 200 is U-shaped bottom, can accommodate 5~20ml liquid, preferably 15ml；Dilution trap 230 is U
Type bottom can accommodate 0.5~2ml liquid, preferably 2ml；Alternatively, dilution holes 220 are U-shaped bottom, orifice diameter is less than bottom hole diameter, can
Accommodate 0.2~2ml liquid, preferably 2ml.Dilution trap 230 (dilution holes 220) has Arabic numerals mark, 230 He of dilution trap
Quantity, shape and the arrangement mode of dilution holes 220 do not provide, but within the application protection.Detection plate 100 and dilution
Disk 200 is connected by buckle-type, i.e., detachably.Detection lid 300 divides for left and right area；Left area indicates opposite with position in detection plate
The Arabic numerals and English capitalization answered, right area are recording areas, can carry out the contents such as sample ID, quantity, test period
Detailed record.
Citing uses demonstration:
It will test the culture that growth in the detection hole 110 in disk 100 has each hole of cell monolayer that 100 μ l serum-frees are added
Liquid.1ml virus is added in the dilution holes 220 of dilution disk 200.900 μ l are added in each hole of dilution holes 220 on dilution disk 200
The culture solution of serum-free, is then added to serum-free medium for virus and does 10 times of gradient dilutions and (take 10-1、10-2……10-10), the virus of each dilution takes 100 μ l to be inoculated into each hole of 100 respective column of detection plate respectively, and each dilution does 8 weights
Multiple, every 100 μ l of hole is placed in 37 DEG C containing 5% CO2After cultivating about 4 in incubator, cytopathy is observed.Using conventional Reed-
Muench method calculates tissue culture infective dose (TCID50).
The above specific embodiment only describes the invention in detail, and those skilled in the art should manage
Solution.Invention can be applied to cell culture, Serologic detection, virology and medical domain, to repairing on the basis of the present invention
Change or equivalent replacement should all cover without departing from mentality of designing and range of the invention in claim model of the invention
In enclosing.
A kind of virus infectivity detection device provided by the embodiment of the utility model, including above-mentioned titer determination device.
Virus infectivity detection device may include incubator, for accommodating titer determination device, and carry out the training of virus
It supports.
Finally, it should be noted that embodiment described above, only specific embodiment of the present utility model, to illustrate this
The technical solution of utility model, rather than its limitations, the protection scope of the utility model is not limited thereto, although referring to aforementioned
The utility model is described in detail in embodiment, those skilled in the art should understand that: it is any to be familiar with this skill
The technical staff in art field within the technical scope disclosed by the utility model, still can be to skill documented by previous embodiment
Art scheme modify or can readily occur in variation or equivalent replacement of some of the technical features；And these modifications,
Variation or replacement, the spirit and model of the utility model embodiment technical solution that it does not separate the essence of the corresponding technical solution
It encloses, should be covered within the scope of the utility model.Therefore, the protection scope of the utility model is answered described is wanted with right
Subject to the protection scope asked.
Claims (7)
1. a kind of titer determination device characterized by comprising detection plate and dilution disk, the detection plate and the dilution disk
It is detachably connected；
Detection hole is provided in the detection plate, the detection hole is for carrying out biochemical reaction；
It is provided on the dilution disk and contains liquid portion and dilution part, for holding dilution, the dilution part is used in the Sheng liquid portion
Carry out the dilution of determinand；
The bottom of the detection hole is flat；
The dilution part includes dilution holes, along the depth direction of the dilution holes, the cross-sectional area of the dilution holes first increases arrive after
Reduce；
The marginal position of the detection plate lower surface is provided with the first recess portion, and the edge of the upper surface of the dilution disk is provided with
Second recess portion prevents the two towards direction away from each other so that the detection plate is connected together with dilution disk up and down motion
Movement.
2. titer determination device according to claim 1, which is characterized in that the quantity of the detection hole is multiple.
3. titer determination device according to claim 1, which is characterized in that along first direction, multiple detection hole edges
Linear interval arrangement, forms gradient detection hole group.
4. titer determination device according to claim 3, which is characterized in that the quantity of the gradient detection hole group is more
Group, and gradient detection hole group described in multiple groups arranges in a second direction, the first direction is vertical with second direction.
5. titer determination device according to claim 4, which is characterized in that the quantity of the dilution holes and the gradient are examined
The quantity of detection hole in gaging hole group is identical, and corresponds；Alternatively,
The dilution part includes dilution trap, the quantity phase of the quantity of the dilution trap and the detection hole in the gradient detection hole group
Together, it and corresponds.
6. titer determination device according to claim 1, which is characterized in that the titer determination device includes detection lid,
The detection lid is for buckle closure in the detection plate and dilution disk.
7. a kind of virus infectivity detection device, which is characterized in that including titer determination as claimed in any one of claims 1 to 6
Device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820241602.XU CN208420822U (en) | 2018-02-09 | 2018-02-09 | Titer determination device and virus infectivity detection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820241602.XU CN208420822U (en) | 2018-02-09 | 2018-02-09 | Titer determination device and virus infectivity detection device |
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| Publication Number | Publication Date |
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| CN208420822U true CN208420822U (en) | 2019-01-22 |
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| CN201820241602.XU Active CN208420822U (en) | 2018-02-09 | 2018-02-09 | Titer determination device and virus infectivity detection device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110082517A (en) * | 2019-03-12 | 2019-08-02 | 宁波美康盛德生物科技有限公司 | A kind of novel agent item |
-
2018
- 2018-02-09 CN CN201820241602.XU patent/CN208420822U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110082517A (en) * | 2019-03-12 | 2019-08-02 | 宁波美康盛德生物科技有限公司 | A kind of novel agent item |
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Effective date of registration: 20191204 Address after: 201210 floor 2-3, building 8, No. 338, Galileo Road, free trade zone, Pudong New Area, Shanghai Patentee after: Tiankang Biology (Shanghai) Co.,Ltd. Address before: No. 528 Changchun South Road, the Xinjiang Uygur Autonomous Region Urumqi high tech Industrial Development Zone, the Xinjiang Uygur Autonomous Region Patentee before: TECON BIOLOGICAL Co.,Ltd. |
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Address after: 215002 station 6-013, 6th floor, No. 88, modern Avenue, Suzhou Industrial Park, Suzhou area, China (Jiangsu) pilot Free Trade Zone, Suzhou City, Jiangsu Province (cluster registration) Patentee after: Tiankang Pharmaceutical (Suzhou) Co.,Ltd. Address before: 2-3 / F, building 8, 338 Galileo Road, Pudong New Area pilot Free Trade Zone, Shanghai 201210 Patentee before: Tiankang Biology (Shanghai) Co.,Ltd. |
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Address after: 215000 station 6-013, 6 / F, No. 88, modern Avenue, Suzhou Industrial Park, Suzhou area, China (Jiangsu) pilot Free Trade Zone, Suzhou, Jiangsu (cluster registration) Patentee after: Tiankang Pharmaceutical Co.,Ltd. Address before: 215002 station 6-013, 6th floor, No. 88, modern Avenue, Suzhou Industrial Park, Suzhou area, China (Jiangsu) pilot Free Trade Zone, Suzhou City, Jiangsu Province (cluster registration) Patentee before: Tiankang Pharmaceutical (Suzhou) Co.,Ltd. |