CN208362348U - A kind of high pressure homogenizer working cycles system - Google Patents
A kind of high pressure homogenizer working cycles system Download PDFInfo
- Publication number
- CN208362348U CN208362348U CN201820559982.1U CN201820559982U CN208362348U CN 208362348 U CN208362348 U CN 208362348U CN 201820559982 U CN201820559982 U CN 201820559982U CN 208362348 U CN208362348 U CN 208362348U
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- China
- Prior art keywords
- high pressure
- pressure homogenizer
- tank
- bacteriolyze
- receiving tank
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Abstract
The utility model discloses a kind of high pressure homogenizer working cycles system, it include bacteriolyze tank, the liquid outlet of bacteriolyze tank is connected with the first drawing liquid pump, homogeneous agitating device is equipped in bacteriolyze tank, first drawing liquid pump is connected with high-order basin, high pressure homogenizer, heat exchanger, receiving tank in turn by connecting pipe, high-order basin and the liquid inlet of receiving tank are respectively equipped with first filter and the second filter, the liquid outlet of receiving tank is connected to the liquid inlet of high-order basin by circulating line, circulating line is equipped with the second drawing liquid pump, and heat exchanger is connected with chilled water system.Compared with prior art, the damage of heat-sensitive materials in cell extract can be effectively reduced in the utility model, and guarantee cell extract is complete as far as possible;Secondly, avoiding the pumping hole blocking of high pressure homogenizer, it ensure that the crushing effect of high pressure homogenizer, reduce the damage number of high pressure homogenizer, reduce the maintenance cost of high pressure homogenizer.
Description
Technical field
The utility model belongs to bioengineering production technical field, particularly, is related to a kind of high pressure homogenizer working cycles
System.
Background technique
High pressure homogenizer is common a kind of equipment in bioengineering, its main function is broken Escherichia coli, yeast
Equal cells, then extract intracellular substance.The principle of its smudge cells is that cell suspending liquid enters in high pressure homogenizer,
Under the action of booster body, quickly through homogeneous chamber, cell can be simultaneously by high speed shear, high frequency oscillation, cavitation and right
Then mechanical forces, the fiting chemical effects such as stream shock make clasmatosis.But since power is larger, cell suspending liquid
When being crushed in homogenizer, a large amount of heat can be absorbed, so that homogenizer breaks off the temperature of the cell extract come
Height is spent, it is impaired so as to cause the heat-sensitive materials of cell extract, it cannot get complete cell extract;Secondly, most of thin
It being installed in plastic tape when born of the same parents' chilled storage, defrosting when, often has plastic flakes and is mixed in cell suspending liquid,
These fragments can result in blockage to the pump head of homogenizer, not only influence the crushing effect of homogenizer, but also will cause homogenizer
Damage, increase maintenance cost.
Utility model content
For overcome the deficiencies in the prior art, the utility model provides a kind of damage for reducing heat-sensitive materials in cell extract
The high pressure homogenizer working cycles system of the maintenance cost of bad, to reduce high pressure homogenizer damage number, reduction high pressure homogenizer.
The technical scheme adopted by the utility model to solve the technical problem is as follows:
A kind of high pressure homogenizer working cycles system includes bacteriolyze tank, high pressure homogenizer;It is equipped in the bacteriolyze tank equal
Matter agitating device further includes having heat exchanger, high-order basin, receiving tank;The liquid outlet of the bacteriolyze tank is connected with the first drawing liquid
Pump, first drawing liquid pump are sequentially connected the high-order basin, the high pressure homogenizer, the heat exchange by connecting pipe
The liquid inlet of device, the receiving tank, the high position basin and the receiving tank is respectively equipped with first filter and the second filter,
The liquid outlet of the receiving tank is connected to the liquid inlet of the high-order basin by circulating line, and the circulating line is equipped with second
Drawing liquid pump, the heat exchanger are connected with chilled water system.
As an improvement of the above scheme, the bacteriolyze tank, the high-order basin, the receiving tank liquid outlet be located at
The bacteriolyze tank, the high-order basin, the receiving tank bottom.
As a further improvement of the foregoing solution, the first filter is mounted on the high-order basin by clamp connection
Top.
Further, second filter is mounted on the top of the receiving tank by clamp connection.
Further, the homogeneous agitating device includes the driving motor of agitator arm, the driving agitator arm rotation.
Further, the driving motor is located at the top of the bacteriolyze tank.
Further, the heat exchanger is lamella heat exchanger.
The utility model has the beneficial effects that compared with prior art, the utility model utilizes heat exchanger and chilled water system
Cooperation by the cell extract transported out from high pressure homogenizer cool down, temperature-sensitive object in cell extract can be effectively reduced
The damage of matter, guarantee cell extract is complete as far as possible;It, can be with secondly, by the first filter that is arranged on high-order basin
Cell suspending liquid is allowed to filter out plastic flakes before entering high pressure homogenizer, it is stifled so as to avoid the pumping hole of high pressure homogenizer
Plug, ensure that the crushing effect of high pressure homogenizer, reduces the damage number of high pressure homogenizer, reduce the dimension of high pressure homogenizer
Accomplish this.
Detailed description of the invention
The present invention will be further described with reference to the accompanying drawings and examples.
Fig. 1 is the overall flow schematic diagram of the utility model.
Specific embodiment
Referring to Fig.1, a kind of high pressure homogenizer working cycles system includes bacteriolyze tank 1, high pressure homogenizer 5;The bacteriolyze
It is equipped with homogeneous agitating device 11 in tank 1, further includes having heat exchanger 6, high-order basin 4, receiving tank 7;The bacteriolyze tank 1 goes out liquid
Mouth is connected with the first drawing liquid pump 2, and first drawing liquid pump 2 is sequentially connected the high-order basin 4, the height by connecting pipe 3
The liquid inlet of pressure homogenizer 5, the heat exchanger 6, the receiving tank 7, the high position basin 4 and the receiving tank 7 is set respectively
There are first filter 41 and the second filter 71, the liquid outlet of the receiving tank 7 is connected to the high-order basin by circulating line 8
4 liquid inlet, the circulating line 8 are equipped with the second drawing liquid pump 81, and the heat exchanger 6 is connected with chilled water system 9.It is described molten
By the homogeneous agitating device 11 cell suspending liquid is made in cell after defrosting by bacterium tank 1.The first filter 41 is used for
It, to filtering out, will be avoided described in blocking from the cell mass of plastic flakes and bulky grain in the cell suspending liquid of the bacteriolyze tank 1
The pumping hole of high pressure homogenizer 5 reduces the damage of the high pressure homogenizer 5 to guarantee the crushing effect of the high pressure homogenizer 5
Number reduces the maintenance cost of the high pressure homogenizer 5.The first filter 41 and second filter 71 are all made of
304 stainless steel filter screens.
The cell extract that the heat exchanger 6 is used to come out from the high pressure homogenizer 5 is cooling, cooling, reduces thin
The damage of heat-sensitive materials in born of the same parents' extracting solution, obtains intact cell extract as far as possible, to improve the utilization rate of raw material.The heat
The cold water tube inlet and cold water pipe of entrance and exit and the chilled water system 9 that exchanger 6 is equipped with cell extract go out
Mouthful, the chilled water system 9 is that the heat exchanger 6 provides the water at low temperature of 2 to 7 degree, is used for cooling cell extracting solution.The reception
Tank 7 is used to receive the cell extract by the high pressure homogenizer 5 after broken, prepares for factory's others process.It is described
The received cell extract of receiving tank 7 need to detect by artificial detection cell extract protein content how much, to examine
The cell of cell extract is surveyed either with or without being broken completely, if not broken, second drawing liquid pump 81 is started to work completely,
By the liquid transporting in the receiving tank 7 into the high-order basin 4, by the primary first filter 41, then again
Carry out a clasmatosis.
Preferably, in order to preferably convey the bacteriolyze tank 1, the high-order basin 4,7 the inside of the receiving tank liquid,
The bacteriolyze tank 1, the high-order basin 4, the receiving tank 7 liquid outlet be located at the bacteriolyze tank 1, the high-order basin
4, the effective use of resource is realized so that the intracorporal liquid of tank can all be flowed out in the bottom of the receiving tank 7.
Preferably, in order to facilitate installation and removal, the Yi Jiwei of the first filter 41 and second filter 71
It is preferably passed through liquid, the first filter 41 is mounted on the top of the high-order basin 4 by clamp connection.Described
Tow filtrator 71 is mounted on the top of the receiving tank 7 by clamp connection.The first filter 41 and second filtering
Device 71 is mounted on the top of corresponding tank body, can allow through the first filter 41 and second filter 71
Liquid filters out the cell mass of plastic flakes and bulky grain in liquid under gravity, avoids blocking described high-pressure homogeneous
The pumping hole of machine 5.
Preferably, the homogeneous agitating device 11 includes the driving of agitator arm 111, the driving rotation of agitator arm 111
Motor 112.The driving motor 112 is located at the top of the bacteriolyze tank 1.The agitator arm 111 is equipped with multiple paddles, uses
In stirring the liquid inside the bacteriolyze tank 1, cell mass, output cell suspending liquid are broken up.
Preferably, the heat exchanger 6 is lamella heat exchanger.The heat exchange coefficient of lamella heat exchanger is big, takes up an area face
Small and easy to clean, the good economical benefit of product.
The workflow of the utility model are as follows: cell is made by being put into the bacteriolyze tank 1 after the eucaryotic cell structure after freezing
Cell suspending liquid in the bacteriolyze tank 1 is pumped into the high-order basin 4, passes through by suspension, subsequent first drawing liquid pump 2
The filtering of the first filter 41 filters out the cell mass and plastic flakes of bulky grain, and then the high pressure homogenizer 5 was incited somebody to action
Cell suspending liquid after filter is pumped into homogeneous chamber, carries out clasmatosis, the high pressure homogenizer 5 broken cell extract warp
It crosses the heat exchanger 6 to be cooled down, then be entered in the receiving tank 7 by the second filter 71.Described in artificial detection
When cell extract in receiving tank 7 is unbroken complete, second drawing liquid pump 81 is started to work, will be in the receiving tank 7
Liquid transporting filters by the first filter 41 into the high-order basin 4, then carries out a clasmatosis again.
When cell extract in the receiving tank 7 described in the artificial detection is crushed complete, then the production of cell extract is completed.
The above is the better embodiment of the utility model, but the utility model is not limited to above-described embodiment,
As long as its technical effect for reaching the utility model with any same or similar means, should all fall into the protection model of the utility model
Within enclosing.
Claims (7)
1. a kind of high pressure homogenizer working cycles system includes bacteriolyze tank (1), high pressure homogenizer (5);The bacteriolyze tank (1)
It is interior to be equipped with homogeneous agitating device (11), it is characterised in that: to further include having heat exchanger (6), high-order basin (4), receiving tank (7);
The liquid outlet of the bacteriolyze tank (1) is connected with the first drawing liquid pump (2), and first drawing liquid pump (2) passes through connecting pipe (3) successively
Connect the high-order basin (4), the high pressure homogenizer (5), the heat exchanger (6), the receiving tank (7), the high position
Basin (4) and the liquid inlet of the receiving tank (7) are respectively equipped with first filter (41) and the second filter (71), the reception
The liquid outlet of tank (7) is connected to the liquid inlet of the high-order basin (4) by circulating line (8), and the circulating line (8) is equipped with
Second drawing liquid pump (81), the heat exchanger (6) are connected with chilled water system (9).
2. a kind of high pressure homogenizer working cycles system according to claim 1, it is characterised in that: the bacteriolyze tank (1),
The high position basin (4), the receiving tank (7) liquid outlet be located at the bacteriolyze tank (1), the high-order basin (4), institute
State the bottom of receiving tank (7).
3. a kind of high pressure homogenizer working cycles system according to claim 1, it is characterised in that: the first filter
(41) top of the high-order basin (4) is mounted on by clamp connection.
4. a kind of high pressure homogenizer working cycles system according to claim 1, it is characterised in that: second filter
(71) top of the receiving tank (7) is mounted on by clamp connection.
5. a kind of high pressure homogenizer working cycles system according to claim 1 to 4, it is characterised in that: the homogeneous
Agitating device (11) includes the driving motor (112) of agitator arm (111), driving agitator arm (111) rotation.
6. a kind of high pressure homogenizer working cycles system according to claim 5, it is characterised in that: the driving motor
(112) it is located at the top of the bacteriolyze tank (1).
7. a kind of high pressure homogenizer working cycles system according to claim 1 to 4, it is characterised in that: the heat is handed over
Parallel operation (6) is lamella heat exchanger.
Priority Applications (1)
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CN201820559982.1U CN208362348U (en) | 2018-04-18 | 2018-04-18 | A kind of high pressure homogenizer working cycles system |
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CN201820559982.1U CN208362348U (en) | 2018-04-18 | 2018-04-18 | A kind of high pressure homogenizer working cycles system |
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CN201820559982.1U Expired - Fee Related CN208362348U (en) | 2018-04-18 | 2018-04-18 | A kind of high pressure homogenizer working cycles system |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110077870A (en) * | 2019-04-15 | 2019-08-02 | 浙江海洋大学 | A kind of improvement microfluidic devices |
CN114874897A (en) * | 2022-07-11 | 2022-08-09 | 南京邦康生物技术有限公司 | Selenium-enriched yeast wall breaking control system and method |
-
2018
- 2018-04-18 CN CN201820559982.1U patent/CN208362348U/en not_active Expired - Fee Related
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110077870A (en) * | 2019-04-15 | 2019-08-02 | 浙江海洋大学 | A kind of improvement microfluidic devices |
CN114874897A (en) * | 2022-07-11 | 2022-08-09 | 南京邦康生物技术有限公司 | Selenium-enriched yeast wall breaking control system and method |
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GR01 | Patent grant | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190111 Termination date: 20210418 |
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CF01 | Termination of patent right due to non-payment of annual fee |