CN208339814U - A kind of novel plasma discharge apparatus that can handle multiple biological samples simultaneously - Google Patents
A kind of novel plasma discharge apparatus that can handle multiple biological samples simultaneously Download PDFInfo
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- CN208339814U CN208339814U CN201721107181.3U CN201721107181U CN208339814U CN 208339814 U CN208339814 U CN 208339814U CN 201721107181 U CN201721107181 U CN 201721107181U CN 208339814 U CN208339814 U CN 208339814U
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Abstract
The utility model relates to the bacteria inactivation rate of chemical engineering, biology and medical domain, reinforcing, mutation breeding apparatus field, specifically a kind of novel plasma discharge apparatus that can handle multiple biological samples simultaneously.The device includes example platform, gas cylinder, plasma electrical source, it further include one-to-one 4 top electrodes and 4 lower electrodes, 4 lower electrodes are built in 4 bottom portion of groove on example platform respectively, 4 top electrodes are separately fixed on an organic glass outer cover, organic glass outer cover is connected by rotary knob rod with example platform, there is an air inlet to be connected on organic glass outer cover with gas cylinder, has venthole with air inlet opposite side；Plasma electrical source further includes power amplifier, is connected respectively with 4 top electrodes.The utility model realizes the diversity and high efficiency of processing biological sample, can handle multiple samples simultaneously or carry out the parallel laboratory test of sample simultaneously, greatly improve the repeatability of plasma-treated samples.
Description
Technical field
The present invention relates to the bacteria inactivation rate of chemical engineering, biology and medical domain, reinforcing, mutation breeding equipment necks
Domain, specifically a kind of novel plasma discharge apparatus that can handle multiple samples simultaneously.
Background technique
Plasma discharge apparatus is provided for the reinforcing of medicine sterilization, microorganism fungus kind mutagenesis and biochemical reaction process
Core equipment.Its structure, mode of operation and operating condition and thallus survival rate, microbial mutation rate and biochemical reaction are strengthened
Effect is closely related.
Atmos low-temperature plasma is the 4th state of substance, and discharge process can produce a large amount of active particles, such as high energy
Electronics, ion, molecule, neutral atom, excited atom, photon and free radical, these active factors have height physics and
Chemical activity, thus had a wide range of applications in fields such as physics, Biochemical Engineering, biology and medicine.
Existing plasma discharge apparatus usually handles biological sample using a pair of of upper/lower electrode, often there is processing sample
The bad phenomenon of product limited amount, experiment repetitive rate.Therefore, how during plasma-treated samples, guarantee experiment
Repeatability is current plasma technique in one of application field urgent problem.
Summary of the invention
To solve the above-mentioned problems, the present invention provides the novel plasma that can handle multiple biological samples simultaneously of one kind and puts
Electric installation.
For achieving the above object, The technical solution adopted by the invention is as follows:
A kind of novel plasma discharge apparatus that can handle multiple biological samples simultaneously, including example platform, gas cylinder, etc.
Gas ions power supply has air valve, air gauge and gas flowmeter on gas cylinder, and plasma electrical source includes electric power host, power supply master
There are power switch and current adjusting knob on machine, it is one-to-one respectively that the plasma discharge apparatus, which includes 4 pairs of electrodes,
4 top electrodes and 4 lower electrodes；Lower electrode is circular metal plate I, and 4 lower electrodes are built in 4 on example platform respectively
Circular groove bottom；Top electrode is made of circular metal plate II, electrode stem, nut I, and circular metal plate II (4) is placed in stone
In English glass outer (7), electrode stem (5) is inserted in polytetrafluoroethylene (PTFE) housing (8), and it is solid that 4 top electrodes pass through 4 nuts I respectively
It is scheduled on a square organic glass outer cover；Organic glass outer cover is connected by rotary knob rod with example platform, and rotary knob rod one end is solid
It is scheduled on example platform, the other end is with the fixed organic glass outer of nut II；There are an air inlet and gas cylinder on organic glass outer cover
It is connected, has a venthole with air inlet opposite side corresponding position；The plasma electrical source further includes power amplifier, power supply
Amplifier can be connected with 4 top electrodes respectively.
There is a fixed plate in above-mentioned organic glass outer cover bosom position, and 4 top electrodes and rotary knob rod are each passed through fixation
Plate.
Metal used in above-mentioned top electrode and lower electrode is one of copper, aluminium, stainless steel.
Gases used in above-mentioned gas cylinder is air, oxygen, nitrogen, helium or argon gas.
The area of above-mentioned example platform is 220cm × 220cm.
The depth of above-mentioned groove is 1cm, groove diameter 5.5cm, 16~20cm of distance between two grooves, each groove away from
From 10~15cm of example platform edge.
Above-mentioned 4 top electrodes are in arranged in parallel.
The diameter of above-mentioned lower electrode circle metal plate I is 5.5cm；II diameter of top electrode circular metal plate is 5.0cm,
16~20cm of distance between top electrode, each top electrode is apart from 10~15cm of example platform edge.
There is hinge on above-mentioned organic glass outer cover, respectively has leader on former and later two outer end faces of organic glass outer cover.
Above-mentioned organic glass outer cover is colorless and transparent.
Compared with prior art, the invention has the benefit that the present invention realize processing biological sample diversity and
High efficiency can handle multiple samples simultaneously or carry out the parallel laboratory test of sample simultaneously, greatly improve plasma-treated samples
Repeatability, guarantee research work from now on accuracy and repeatability.The present invention is in plasma technique, biochemical industry, doctor
The fields such as medicine and Fermentation Engineering are with a wide range of applications.
Detailed description of the invention
Fig. 1: schematic structural diagram of the device of the invention；
Fig. 2: top electrode structural schematic diagram of the present invention；
1. example platform in figure, 2. circular metal plates I, 3. grooves, 4. circular metal plates II, 5. electrode stems, 6. spiral shells
Mother I, 7. quartz glass outer covers, 8. polytetrafluoroethylene (PTFE) housings, 9. organic glass outer covers, 10. rotary knob rods, 11. nuts II, 12. is solid
Fixed board, 13. air inlets, 14. gas cylinders, 15. ventholes, 16. air valves, 17. air gauges, 18. gas flowmeters, 19. electric power hosts,
20. power switch, 21. current adjusting knobs, 22. power amplifiers, 23. hinges, 24. handles.
Specific embodiment
The present invention will be further described With reference to embodiment.
A kind of novel plasma discharge apparatus that can handle multiple biological samples simultaneously, including example platform 1, gas cylinder
14, plasma electrical source, the area of example platform are 220cm × 220cm, and it is gentle to have air valve 16, air gauge 17 on gas cylinder 14
Flowmeter body 18, plasma electrical source include electric power host 19, there is power switch 20 and current adjusting knob 21 on electric power host,
Described device includes 4 pairs of electrodes, is one-to-one 4 top electrodes and 4 lower electrodes respectively；Lower electrode is circular metal plate
I 2, a diameter of 5.5cm, 4 lower electrodes are built in 43 bottoms of groove on sample stage, depth of groove 1cm, groove respectively
Diameter 5.5cm, 16~20cm of distance, each groove distance example platform 10~15cm of edge between two grooves；Top electrode by
Circular metal plate II 4, electrode stem 5, nut I 6 form, and II 4 diameter of circular metal plate is 5.0cm, circular metal plate II 4
It is placed in quartz glass outer cover 7, electrode stem 5 is inserted in polytetrafluoroethylene (PTFE) housing 8, and electrode stem 5 has external screw thread, 4 top electrodes
It is fixed on a square organic glass outer cover 9 by nut I 6 respectively, 4 top electrodes are in arranged in parallel, the spacing of top electrode
From 16~20cm, each top electrode is apart from 10~15cm of example platform edge.Metal used in top electrode and lower electrode be copper, aluminium,
One of stainless steel.Organic glass outer cover 9 is connected by rotary knob rod 10 with example platform 1, and rotary knob rod 10 has external screw thread, rotation
10 one end of button bar is fixed on example platform 1, and the other end is passed through with the fixed organic glass outer 9 of nut II 11, plexiglass tent 9
It rotates rotary knob rod 10 and adjusts cover integral elevating, thus the lifting of 4 top electrodes of drive, final fixed top electrode and lower electrode
Spacing；9 bosom position of organic glass outer cover has a fixed plate 12 for reinforcing plexiglass tent 9,4 top electrodes and knobs
Bar 10 is each passed through fixed plate 12.There is an air inlet 13 to be connected by hose with gas cylinder 14 on organic glass outer cover 9, with air inlet
13 opposite side corresponding position of hole has a venthole 15, and air inflow is controlled by air valve 16, air gauge 17 and gas flowmeter 18, gas
Gases used in bottle is air, oxygen, nitrogen, helium or argon gas；The plasma electrical source further includes power amplifier 22, electricity
Source amplifier can be connected with 4 top electrodes respectively, be needed that power amplifier is selected to connect 1,2,3 or 4 according to experiment
Top electrode.There are four hinge 23 on organic glass outer cover 9, respectively there is a handle on former and later two outer end faces of organic glass outer cover
24, it is used to open glass outer, places sample.
During the experiment, sample to be processed is placed in groove on lower electrode, organic glass is adjusted by rotary knob rod
Electrode needs to be passed through air, oxygen according to experiment at a distance from top electrode under lift adjustment of the outer cover lifting to drive top electrode
Gas, nitrogen, helium or argon gas turn on the power switch startup power supply host, are needed according to experiment by power amplifier and experiment institute
Have access to electricity and be extremely connected, it is real to specifying to test voltage, then the current adjusting knob adjusted on electric power host to adjust power amplifier
Galvanoscope, upper and lower electrode is powered and then gas discharge generates required plasma and starts to test.
Embodiment 1
(1) atmospheric dielectric barrier discharge cold plasma parallel pole influences saccharomyces cerevisiae inactivation ratio
Strain: saccharomyces cerevisiae (Saccharomyces cerevisiae) is by this seminar voluntarily screening and identification, and preservation
In China General Microbiological culture presevation administrative center (CGMCC), culture presevation number is CGMCC no.6184.
Bacterium solution preparation: the strain of Storage in refrigerator is accessed into another fresh inclined-plane YPD culture medium, 30 DEG C of cultures 24~48 are small
When, it is then inoculated in YPD fluid nutrient medium, 30 DEG C are cultivated 18~20 hours, and fresh YPD culture solution adjusts bacterial concentration OD600=
2.0。
1.1 air plasma inactivation experiments: 2 parts of 3mL bacterium solutions are respectively placed in the quartzy culture dish of diameter 5cm,
It is respectively placed on the lower electrode of 2 grooves of example platform, adjusts rotary knob rod, top electrode is apart from bacterium solution surface 3mm, atmospherical discharges.
The dyeing of placenta indigo plant, living cell counting quantity and dead cell quantity, calculate thallus lethality: lethality=(dead by formula respectively
Cell quantity/total number of cells) × 100%.The results are shown in Table 1, and each processing group cell lethality is significantly higher than control, this explanation
Apparatus of the present invention atmospherical discharges have significant ground killing effect to saccharomyces cerevisiae；Two pairs of parallel poles are in identical discharge parameter condition
Under, cell lethality there are no significant difference, the result shows two pairs of electrodes can be used as parallel pole, for parallel real
It tests, to realize the repeatability of experimental data.
The comparison that 1 atmospherical discharges plasma two of table influences parallel pole on microorganism lethality
aP < 0.005,b P<0.001
1.2 nitrogen gas plasma inactivation experiments: 4 parts of 3mL bacterium solutions are respectively placed in the quartzy culture dish of diameter 5cm,
It is respectively placed in 4 grooves of example platform on lower electrode, adjusts rotary knob rod, top electrode is apart from bacterium solution surface 3mm, nitrogen discharge.
The dyeing of placenta indigo plant, living cell counting quantity and dead cell quantity, calculate thallus lethality: lethality=(dead by formula respectively
Cell quantity/total number of cells) × 100%.The results are shown in Table 2, and each processing group cell lethality is significantly higher than control, this explanation
The device nitrogen discharge has significant ground killing effect to saccharomyces cerevisiae；Four pairs of parallel poles under the conditions of unified discharge parameter,
Cell lethality there are no significant difference, the result shows four pairs of electrodes can be used as parallel pole, is used for parallel laboratory test, from
And realize the repeatability of experimental data.
The comparison that 2 helium discharge plasma four of table influences parallel pole on microorganism lethality
aP < 0.005,b P<0.001
Embodiment 2
(2) atmospheric dielectric barrier discharge cold plasma parallel pole is to saccharomyces cerevisiae activation effect
Strain: saccharomyces cerevisiae (Saccharomyces cerevisiae) is by this seminar voluntarily screening and identification, and preservation
In China General Microbiological culture presevation administrative center (CGMCC), culture presevation number is CGMCC no.6184.
Bacterium solution preparation: the strain of Storage in refrigerator is accessed into another fresh inclined-plane YPD culture medium, 30 DEG C of cultures 24~48 are small
When, it is then inoculated in YPD fluid nutrient medium, 30 DEG C are cultivated 18~20 hours, and fresh YPD culture solution adjusts bacterial concentration OD600=
2.0.2.1 air plasma activation experiments: 2 parts of 1mL bacterium solutions are respectively placed in the quartzy culture dish of diameter 5cm, respectively
It is placed on the lower electrode of 2 grooves of example platform, adjusts rotary knob rod, make top electrode apart from bacterium solution surface 3mm, atmospherical discharges.It will
It handles somatic cells to count, adjusts each processing group in same cell concentration (1 × 107), YPD is inoculated in 1% inoculum concentration respectively
Fluid nutrient medium, 30 DEG C of shaking flask cultures are for 24 hours.Fermentation latter stage unit cell biomass (unit OD is calculated by formula600), unit
OD600=fermentation latter stage OD600/1×107Cell.The results are shown in Table 3, and when discharge power is 54W, plasma two parallel
Electrode handles Oscillating bottles of the saccharomyces cerevisiae inoculum Jing fermentations of 3min for 24 hours respectively, their fermentation latter stage unit OD600It is significantly high respectively
In control group.After the inoculum fermentation of two pairs of parallel pole processing, unit OD600In 0.005 and 0.001 level without conspicuousness
Difference.The above results show that atmospheric air dielectric barrier discharge has saccharomyces cerevisiae inoculum to strengthen and swash
Active is answered, meanwhile, the experimental result of two pairs of parallel poles is consistent, and can be used for the repeated work of parallel laboratory test.
Comparison of the 3 atmospherical discharges plasma two of table to parallel pole to microorganism activation effect
aP < 0.005,b P<0.001
2.2 nitrogen gas plasma activation experiments: 4 parts of 1mL bacterium solutions are respectively placed in the quartzy culture dish of diameter 5cm,
It is respectively placed on the lower electrode of 4 grooves of example platform, adjusts rotary knob rod, top electrode is apart from bacterium solution surface 3mm, nitrogen discharge.
Processing somatic cells are counted, adjust each processing group in same cell concentration (1 × 107), it is inoculated in respectively with 1% inoculum concentration
YPD fluid nutrient medium, 30 DEG C of shaking flask cultures.Fermentation latter stage unit cell biomass (unit OD is calculated by formula600), unit
OD600=fermentation latter stage OD600/1×107Cell.The results are shown in Table 4, and when discharge power is 36W, plasma four parallel
Electrode handles Oscillating bottles of the saccharomyces cerevisiae inoculum Jing fermentations of 1min for 24 hours respectively, their fermentation latter stage unit OD600It is significantly high respectively
In control group.After the inoculum fermentation of four pairs of parallel pole processing, unit OD600In 0.001 level, there was no significant difference.On
State the result shows that, atmospheric air dielectric barrier discharge to saccharomyces cerevisiae inoculum have strengthen activation effect,
Meanwhile the experimental result of four pairs of parallel poles is consistent, and can be used for the repeated work of parallel laboratory test.
Comparison of the 4 helium discharge plasma four of table to parallel pole to microorganism activation effect
aP < 0.005,b P<0.001。
Claims (10)
1. a kind of novel plasma discharge apparatus that can handle multiple biological samples simultaneously, including example platform (1), gas cylinder
(14), plasma electrical source, with air valve (16), air gauge (17) and gas flowmeter (18), plasma on gas cylinder (14)
Power supply includes electric power host (19), has power switch (20) and current adjusting knob (21) on electric power host, which is characterized in that institute
Stating plasma discharge apparatus includes 4 pairs of electrodes, is one-to-one 4 top electrodes and 4 lower electrodes respectively；Lower electrode is circle
Shape metal plate I (2), 4 lower electrodes are built in 4 circular groove (3) bottoms on example platform (1) respectively；Top electrode by
Circular metal plate II (4), electrode stem (5), nut I (6) composition, circular metal plate II (4) are placed in quartz glass outer cover (7)
Interior, electrode stem (5) is inserted in polytetrafluoroethylene (PTFE) housing (8), and 4 top electrodes pass through 4 nuts I (6) respectively and are fixed on a pros
On body organic glass outer cover (9)；Organic glass outer cover (9) is connected by rotary knob rod (10) with example platform (1), rotary knob rod (10)
One end is fixed on example platform (1), and the other end is with the fixed organic glass outer (9) of nut II (11)；Organic glass outer cover (9)
On there is an air inlet (13) to be connected with gas cylinder (14), have a venthole (15) with air inlet (13) opposite side corresponding position；Institute
Stating plasma electrical source further includes power amplifier (22), and power amplifier (22) can be connected with 4 top electrodes respectively.
2. a kind of novel plasma discharge apparatus that can handle multiple biological samples simultaneously as described in claim 1, special
Sign is that organic glass outer cover (9) bosom position has a fixed plate (12), and 4 top electrodes and rotary knob rod (10) are worn respectively
Cross fixed plate (12).
3. a kind of novel plasma discharge apparatus that can handle multiple biological samples simultaneously as described in claim 1, special
Sign is that metal used in top electrode and lower electrode is one of copper, aluminium, stainless steel.
4. a kind of novel plasma discharge apparatus that can handle multiple biological samples simultaneously as described in claim 1, special
Sign is that gases used in gas cylinder (14) is air, oxygen, nitrogen, helium or argon gas.
5. a kind of novel plasma discharge apparatus that can handle multiple biological samples simultaneously as described in claim 1, special
Sign is that the area of example platform (1) is 220cm × 220cm.
6. a kind of novel plasma discharge apparatus that can handle multiple biological samples simultaneously as described in claim 1, special
Sign is, the depth of groove (3) is 1cm, groove (3) diameter 5.5cm, 16~20cm of distance between two grooves, each groove
Apart from 10~15cm of example platform edge.
7. a kind of novel plasma discharge apparatus that can handle multiple biological samples simultaneously as described in claim 1, special
Sign is that 4 top electrodes are in arranged in parallel.
8. a kind of novel plasma discharge apparatus that can handle multiple biological samples simultaneously as described in claim 1, special
Sign is that lower I (2) diameter of electrode circle metal plate is 5.5cm；The diameter of top electrode circular metal plate II (4) is
5.0cm, 16~20cm of distance between top electrode, each top electrode is apart from 10~15cm of example platform edge.
9. a kind of novel plasma discharge apparatus that can handle multiple biological samples simultaneously as described in claim 1, special
Sign is have on organic glass outer cover (9) hinge (23), respectively has leader on organic glass outer cover (9) former and later two outer end faces
(24)。
10. a kind of novel plasma discharge apparatus that can handle multiple biological samples simultaneously as described in claim 1, special
Sign is that organic glass outer cover (9) is colorless and transparent.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107412812A (en) * | 2017-08-31 | 2017-12-01 | 大连大学 | A kind of new plasma discharge apparatus that can handle multiple biological samples simultaneously |
| CN115161166A (en) * | 2022-07-27 | 2022-10-11 | 安徽大学 | Experimental device for low temperature plasma inactivation microorganism |
-
2017
- 2017-08-31 CN CN201721107181.3U patent/CN208339814U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107412812A (en) * | 2017-08-31 | 2017-12-01 | 大连大学 | A kind of new plasma discharge apparatus that can handle multiple biological samples simultaneously |
| CN115161166A (en) * | 2022-07-27 | 2022-10-11 | 安徽大学 | Experimental device for low temperature plasma inactivation microorganism |
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