CN208297522U - A kind of ELISA kit - Google Patents
A kind of ELISA kit Download PDFInfo
- Publication number
- CN208297522U CN208297522U CN201821073022.0U CN201821073022U CN208297522U CN 208297522 U CN208297522 U CN 208297522U CN 201821073022 U CN201821073022 U CN 201821073022U CN 208297522 U CN208297522 U CN 208297522U
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- temperature
- conductive layer
- elisa
- graphene
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Abstract
The utility model belongs to field of biotechnology, and in particular to a kind of ELISA kit.ELISA kit provided by the utility model is by 96 hole elisa Plates plate main bodys 1, temperature regulating device 2 and the connection composition of attaching plug 3.Specifically: 96 hole elisa Plates main bodys and graphene conductive layer with graphene conductive layer are electrically connected, the attaching plug for controlling the temperature regulating device of the graphene conductive layer temperature and being electrically connected with temperature regulating device.ELISA kit provided by the utility model can heat ELISA Plate therein by graphene layer, and graphene layer has strong applicability, high temperature resistant, corrosion-resistant, high-temperature stability, electric conductivity is stablized, the advantage that institute's expense is few and coating is thin and electric heating conversion efficiency is high is, it can be achieved that be rapidly heated.It is tested for ELISA, insulating box can be saved and be incubated for process, simplify ELISA operating process, and easy to use.
Description
Technical field
The utility model belongs to field of biotechnology, and in particular to a kind of ELISA kit.
Background technique
Graphene is the two dimensional crystal for the only one layer of atomic thickness being made of carbon atom.Since discovery in 2004,
Operation strategies are being expanded always.The excellent electric property of graphene, electric heating ability outstanding, huge specific surface area and high Young
Modulus is all used for it and provides basis.
The thermal coefficient of graphene is up to 5300W/mK, is higher than carbon nanotube and diamond, its electron transfer under room temperature
Rate is more than 15000cm2/Vs, and higher than carbon nanotubes or silicon crystal, and resistivity only about 10-6 Ω cm, than copper or silver more
It is low, it is the smallest material of world resistivity.
The reaction process of ELISA (enzyme-linked immunosorbent assay) occurs under the conditions of 37 DEG C, as antigen coat, closing,
The processes such as antibody combination.Current operation needs to carry out the operation such as reagent adding, washing on testing stand, is then placed in 37 DEG C of constant temperature
It is heated in case.Since process is complex, the operation for being constantly brought into, taking out along with insulating box in ELISA experimentation, this is not
Only make complicated for operationization, also increases the possibility of pollution.The ELISA kit that can directly heat in ELISA Plate will solve
Certainly problem above, and heating needs while meeting heating rapidly, uniformly in ELISA Plate, and does not influence the item to absorbance value
Part.
Currently, heating unit uses the materials such as metal foil, resistance wire, conductive coating more in the electric heating system used.Stone
The superior function of black alkene surmounts metal material in many aspects, it has performance not available for other metallic heating bodies, corrosion resistant
Erosion, high-temperature stability, electric conductivity is stable, institute's expense is few and coating is thin, electric heating conversion efficiency is high etc., and graphene is almost
Fully transparent, only absorb 2.3% light.It is prominent to apply to electric heating system advantage.
Therefore a kind of ELISA Plate with graphene conductive layer is developed in ELISA kit.
Utility model content
Graphene is applied to ELISA kit, it is heated to ELISA Plate, keeps 37 DEG C of temperature constant, can avoid more
The incubation process of secondary insulating box simplifies experimental implementation process.
In view of this, the purpose of this utility model is to provide a kind of ELISA kit, it can be by graphene layer to it
In ELISA Plate heating, and graphene layer have strong applicability, high temperature resistant is corrosion-resistant, high-temperature stability, electric conductivity stablize,
The advantage that institute's expense is few and coating is thin and electric heating conversion efficiency is high is, it can be achieved that be rapidly heated.
To achieve the above object, the technical solution of the utility model are as follows:
A kind of ELISA kit characterized by comprising
96 hole elisa Plates main bodys and graphene conductive layer with graphene conductive layer are electrically connected, described for controlling
The temperature regulating device of graphene conductive layer temperature and the attaching plug being electrically connected with temperature regulating device；
Wherein graphene conductive layer is detachably connected with temperature regulating device；
The 96 hole elisa Plates main bodys with graphene layer include superficial layer, transparent graphene conductive layer and transparent base
Material；
The superficial layer is that all can make the substance of carrier in enzyme-linked immunosorbent assay；
The temperature regulating device includes temperature detecting module and control module；
The temperature detecting module is used to detect the real time temperature data of graphene layer, and the real time temperature data are passed
It is handed to the control module；
The control module is used to be arranged the control instruction to temperature, and receives the real-time temperature of the temperature detecting module
Degree evidence, and corresponding control command is extracted according to the control instruction and the real time temperature data, the control command is used
In controlling the graphene conductive layer heating or stopping heating, so that the real time temperature data are corresponding with the control instruction
Temperature data match.
The control module of temperature regulating device issues instruction and heats to graphene conductive layer after temperature is arranged, graphene conductive layer
It is heating up, and ELISA Plate is conducted heat to, it is heated.
As a preferred option, above-mentioned temperature detecting module includes temperature sensor, and the temperature sensor is at least arranged
Be spaced between hole on edge in 96 hole elisa Plates main bodys, plate or hole wall in one at.
As a preferred option, the transparent graphene conductive layer of above-mentioned 96 hole elisa Plates main body be located at the transparent substrate and
Between superficial layer.
As a preferred option, above-mentioned 96 hole elisa Plates main body also has plate lid.
As a preferred option, the above-mentioned 96 hole elisa Plates main body numbers with graphene layer are greater than one.
As a preferred option, above-mentioned superficial layer is polystyrene.
As a preferred option, above-mentioned temperature regulating device also includes data transmission module, and the data transmission module is for connecing
It receives the control instruction of external handheld device and the control instruction is transferred to the control module.
The control process specifically: the control module is extracted according to the control instruction and the real time temperature data
Corresponding control command, the control command is for controlling the graphene conductive layer heating or stopping heating, so that described
Real time temperature data temperature data corresponding with the control instruction matches.
The utility model has the beneficial effects that: ELISA kit provided by the utility model:
1) ELISA Plate can be directly heated in ELISA experimentation, and then is saved and taken in multiple insulating box, is heated
Journey.It is tested for ELISA, insulating box can be saved and be incubated for process, enormously simplify the experimental implementation of ELISA.
2) transparent graphene conductive layer has strong applicability, corrosion-resistant, high-temperature stability, and electric conductivity is stablized, required use
Amount is less with coating is thin and electric heating conversion efficiency is high advantage, it can be achieved that be rapidly heated.
3) graphene conductive layer is thin, transparent, will not detect and impact to experimental result.
Detailed description of the invention
Fig. 1 is the ELISA kit schematic diagram of the utility model.
Specific embodiment
The preferred embodiment of (referring to attached drawing) to the utility model is described in detail below.It is not infused in preferred embodiment
The experimental method of bright actual conditions, usually according to normal condition, illustrated embodiment are in order to preferably in the utility model
Appearance is illustrated, but is not that the content of the utility model is only limitted to illustrated embodiment.So those skilled in the art
Nonessential modifications and adaptations are carried out to embodiment according to above-mentioned utility model content, still fall within the protection model of the utility model
It encloses.
1 ELISA kit 1 of embodiment
1. structure
It is as shown in Figure 1 that the utility model develops ELISA heating kit.Include main body 1, temperature regulating device 2, plug 3.Specifically
Are as follows: 96 hole elisa Plates main bodys and graphene conductive layer with graphene conductive layer are electrically connected, for controlling the graphene
The temperature regulating device of conductive layer temperature and the attaching plug being electrically connected with temperature regulating device.
1) heatable 96 hole elisa Plates main body 1 comprising superficial layer, transparent graphene conductive layer and transparent substrate.Table
Surface layer is the immobilization carrier that all can be used for ELISA experiment.
As a preferred option, superficial layer is polystyrene.
As a preferred option, transparent graphene conductive layer is between the transparent substrate and superficial layer.
As a preferred option, heatable 96 hole elisa Plates main body 1 also includes plate lid 13.
As a preferred option, transparent graphene conductive layer is detachably connected with temperature regulating device 2, and main body 1 in kit
Number is greater than 1.Therefore, another main body 1 is changed after use, can be used for multiple ELISA experiment.
2) it is electrically connected with graphene conductive layer, for controlling the temperature regulating device of the graphene conductive layer temperature, including
Temperature detecting module and control module；
The temperature detecting module is used to detect the real time temperature data of graphene layer, and the real time temperature data are passed
It is handed to the control module；
The control module is used to be arranged the control instruction to temperature, and receives the real-time temperature of the temperature detecting module
Degree evidence, and corresponding control command is extracted according to the control instruction and the real time temperature data, the control command is used
In controlling the graphene conductive layer heating or stopping heating, so that the real time temperature data are corresponding with the control instruction
Temperature data match.
As a preferred option, above-mentioned temperature detecting module includes temperature sensor, and the temperature sensor is at least arranged
Be spaced between hole on edge in 96 hole elisa Plates main bodys, plate or hole wall in one at.
1. the control module of temperature regulating device issues instruction and heats to graphene conductive layer after temperature is arranged, graphene conductive
Layer is heating up, and conducts heat to ELISA Plate main body, increases ELISA Plate temperature, further transfers heat to reagent in hole, right
It is heated.
2. the temperature sensor of ELISA Plate main body detects temperature change and is transferred to control module；Until temperature sensor
The temperature of detection reaches setting value, and such as 37 degrees Celsius, stopping call for heat being extracted and issued to control module after receiving temperature data,
Graphene conductive layer stops heating.
2. using
1) before carrying out ELISA experiment, ELISA kit is assembled according to Fig. 1.
Plug connects power supply, and temperature regulating device 2 is arranged in advance, is configured to the temperature of kit, and such as 37 DEG C.When setting temperature
The control module of temperature regulating device issues instruction and heats to graphene conductive layer afterwards, and graphene conductive layer is heating up, and by heat
Conduction heats it to ELISA Plate.The temperature regulating device of the utility model ensure that the stabilization of temperature.
2) ELISA experimental implementation is carried out by step, during insulation reaction, splice lid 13, in favor of heat preservation.
3) main body 1 after the reaction was completed, is removed, is detected for result.The increased transparent graphene of mainboard 1 in the utility model
Conductive layer be almost it is fully transparent, on the progress of experiment without influence.
When experiment next time, another main body 1 is taken, and be assembled into kit shown in Fig. 1.
Finally, it is stated that above embodiments are merely intended for describing the technical solutions of the present application, but not for limiting the present application, although ginseng
The utility model is described in detail according to preferred embodiment, those skilled in the art should understand that, it can be to this
The technical solution of utility model is modified or replaced equivalently, without departing from the objective and model of technical solutions of the utility model
It encloses, should all cover in the scope of the claims of the utility model.
Claims (6)
1. a kind of ELISA kit characterized by comprising
96 hole elisa Plates main bodys and graphene conductive layer with graphene conductive layer are electrically connected, for controlling the graphite
The temperature regulating device of alkene conductive layer temperature and the attaching plug being electrically connected with temperature regulating device；
Wherein graphene conductive layer is detachably connected with temperature regulating device；
The 96 hole elisa Plates main bodys with graphene layer include superficial layer, transparent graphene conductive layer and transparent substrate；
The superficial layer is that all can make the substance of carrier in enzyme-linked immunosorbent assay；
The temperature regulating device includes temperature detecting module and control module；
The temperature detecting module is used to detect the real time temperature data of graphene layer, and the real time temperature data are transferred to
The control module；
The control module is used to be arranged the control instruction to temperature, and receives the real time temperature number of the temperature detecting module
According to, and corresponding control command is extracted according to the control instruction and the real time temperature data, the control command is for controlling
It makes the graphene conductive layer heating or stops heating, so that real time temperature data temperature corresponding with the control instruction
Degree evidence matches.
2. ELISA kit according to claim 1, which is characterized in that the temperature detecting module includes temperature sensing
Device, the temperature sensor be at least set to edge in 96 hole elisa Plates main bodys, on plate between hole interval or hole wall in one
Place.
3. ELISA kit according to claim 1, which is characterized in that the transparent graphite of the 96 hole elisa Plates main body
Alkene conductive layer is between the transparent substrate and superficial layer.
4. ELISA kit according to claim 1, which is characterized in that the 96 hole elisa Plates main body also has plate lid.
5. ELISA kit according to claim 1, which is characterized in that the 96 hole enzymes with graphene conductive layer
Target main body number is greater than one.
6. ELISA kit according to claim 1, which is characterized in that the superficial layer is polystyrene.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821073022.0U CN208297522U (en) | 2018-07-05 | 2018-07-05 | A kind of ELISA kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821073022.0U CN208297522U (en) | 2018-07-05 | 2018-07-05 | A kind of ELISA kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208297522U true CN208297522U (en) | 2018-12-28 |
Family
ID=64701051
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821073022.0U Active CN208297522U (en) | 2018-07-05 | 2018-07-05 | A kind of ELISA kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208297522U (en) |
-
2018
- 2018-07-05 CN CN201821073022.0U patent/CN208297522U/en active Active
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