CN208297521U - A kind of ELISA kit - Google Patents
A kind of ELISA kit Download PDFInfo
- Publication number
- CN208297521U CN208297521U CN201821059079.5U CN201821059079U CN208297521U CN 208297521 U CN208297521 U CN 208297521U CN 201821059079 U CN201821059079 U CN 201821059079U CN 208297521 U CN208297521 U CN 208297521U
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- temperature
- elisa
- main body
- conductive layer
- elisa plate
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Abstract
The utility model belongs to field of biotechnology, and in particular to a kind of ELISA kit.ELISA kit provided by the utility model is by reagent box main body 1, temperature regulating device 2, attaching plug 3 and the connection composition of ELISA Plate 4.Specifically: reagent box wall has the reagent box main body of graphene conductive layer, is electrically connected with graphene conductive layer, for controlling the temperature regulating device, the attaching plug being electrically connected with temperature regulating device, the ELISA Plate 4 being detachably connected with reagent box main body of the graphene conductive layer temperature.ELISA kit provided by the utility model can heat ELISA Plate therein by graphene layer, and graphene layer has strong applicability, high temperature resistant, corrosion-resistant, high-temperature stability, electric conductivity is stablized, the advantage that institute's expense is few and coating is thin and electric heating conversion efficiency is high is, it can be achieved that be rapidly heated.ELISA Plate is detachable, therefore this kit is used multiple times, to reduce cost.It is tested for ELISA, insulating box can be saved and be incubated for process, simplify ELISA operating process, and easy to use.
Description
Technical field
The utility model belongs to field of biotechnology, and in particular to a kind of ELISA kit.
Background technique
Graphene is the two dimensional crystal for the only one layer of atomic thickness being made of carbon atom.Since discovery in 2004,
Operation strategies are being expanded always.The excellent electric property of graphene, electric heating ability outstanding, huge specific surface area and high Young
Modulus is all used for it and provides basis.
The thermal coefficient of graphene is up to 5300W/mK, is higher than carbon nanotube and diamond, its electron transfer under room temperature
Rate is more than 15000cm2/Vs, and higher than carbon nanotubes or silicon crystal, and resistivity only about 10-6 Ω cm, than copper or silver more
It is low, it is the smallest material of world resistivity.
The reaction process of ELISA (enzyme-linked immunosorbent assay) occurs under the conditions of 37 DEG C, as antigen coat, closing,
The processes such as antibody combination.Current operation needs to carry out the operation such as reagent adding, washing on testing stand, is then placed in 37 DEG C of constant temperature
It is heated in case.Since process is complex, the operation for being constantly brought into, taking out along with insulating box in ELISA experimentation, this is not
Only make complicated for operationization, also increases the possibility of pollution.The ELISA kit that can directly heat in ELISA Plate will solve
Certainly problem above, and heating needs while meeting heating rapidly, uniformly in ELISA Plate, and does not influence the item to absorbance value
Part.
Currently, heating unit uses the materials such as metal foil, resistance wire, conductive coating more in the electric heating system used.Stone
The superior function of black alkene surmounts metal material in many aspects, it has performance not available for other metallic heating bodies, corrosion resistant
Erosion, high-temperature stability, electric conductivity is stable, institute's expense is few and coating is thin, electric heating conversion efficiency is high etc., and graphene is almost
Fully transparent, only absorb 2.3% light.It is prominent to apply to electric heating system advantage.
Therefore a kind of ELISA Plate with graphene conductive layer is developed in ELISA kit.
Utility model content
Graphene is applied to ELISA kit, it is heated to ELISA Plate, keeps 37 DEG C of temperature constant, can avoid more
The incubation process of secondary insulating box simplifies experimental implementation process.
In view of this, the purpose of this utility model is to provide a kind of ELISA kit, it can be by graphene layer to it
In ELISA Plate heating, and graphene layer have strong applicability, high temperature resistant is corrosion-resistant, high-temperature stability, electric conductivity stablize,
The advantage that institute's expense is few and coating is thin and electric heating conversion efficiency is high is, it can be achieved that be rapidly heated.
To achieve the above object, the technical solution of the utility model are as follows:
A kind of ELISA kit comprising:
Kit inner wall has the reagent box main body of graphene conductive layer, is electrically connected with graphene conductive layer, for controlling
The temperature regulating device for making the graphene conductive layer temperature, the attaching plug and ELISA Plate being electrically connected with temperature regulating device；Wherein institute
Graphene conductive layer is stated to be fixedly connected with the temperature regulating device；
The top vacancy of the reagent box main body, and it is mounted with the ELISA Plate, the ELISA Plate and the kit master
Body is detachably connected；
The graphene conductive layer is located at the inner surface of the reagent box main body；
The temperature regulating device includes temperature detecting module and control module；
The temperature detecting module is used to detect the real time temperature data of graphene layer, and the real time temperature data are passed
It is handed to the control module；
The control module is used to be arranged the control instruction to temperature, and receives the real-time temperature of the temperature detecting module
Degree evidence, and corresponding control command is extracted according to the control instruction and the real time temperature data, the control command is used
In controlling the graphene conductive layer heating or stopping heating, so that the real time temperature data are corresponding with the control instruction
Temperature data match.
The control module of temperature regulating device issues instruction and heats to graphene conductive layer after temperature is arranged, graphene conductive layer
It is heating up, and reagent box main body is conducted heat to, temperature in reagent box main body is increased, ELISA Plate is further transferred heat to,
It is heated.
As a preferred option, the temperature detecting module includes temperature sensor, and the temperature sensor is at least arranged
In the inner wall of reagent box main body one at.
As a preferred option, the bottom hole of the ELISA Plate and the wall bottom of the reagent box main body are in contact.
When the two is in contact, directly heats and existed simultaneously with air heat transfer: the graphene conductive of kit main body inner wall
ELISA Plate bottom hole can be heated after layer heating, and then transfer heat to reagent in hole, reagent heats in device to hole；Meanwhile graphene conductive
In layer heating kit after air, the part that ELISA Plate is contacted with air is heated.
As a preferred option, the reagent box main body also has box cover.
As a preferred option, the ELISA Plate is 96 hole elisa Plates.
As a preferred option, the ELISA Plate is removable ELISA Plate.
Above-mentioned removable ELISA Plate also includes multiple enzyme mark strips in addition to ELISA Plate main body.
The utility model has the beneficial effects that: ELISA kit provided by the utility model:
1) ELISA Plate can be heated in ELISA experimentation, and then is saved and taken in multiple insulating box, heating process.With
It is tested in ELISA, insulating box can be saved and be incubated for process, enormously simplify the experimental implementation of ELISA.
2) transparent graphene conductive layer has strong applicability, corrosion-resistant, high-temperature stability, and electric conductivity is stablized, required use
Amount is less with coating is thin and electric heating conversion efficiency is high advantage, it can be achieved that be rapidly heated.
3) ELISA Plate is detachably connected with reagent box main body, is made convenient for the detection of subsequent result and kit are repeatable
With.
Detailed description of the invention
Fig. 1 is ELISA kit schematic diagram.Wherein, 1: reagent box main body；11: reagent box cover；2: temperature regulating device；3: electricity
Source plug；4:96 hole elisa Plates.
Fig. 2 is reagent box main body schematic top plan view.Wherein, 1: reagent box main body；4:96 hole elisa Plates；41: ELISA Plate
Partition；42: ELISA Plate hole.
Specific embodiment
The preferred embodiment of (referring to attached drawing) to the utility model is described in detail below.It is not infused in preferred embodiment
The experimental method of bright actual conditions, usually according to normal condition, illustrated embodiment are in order to preferably in the utility model
Appearance is illustrated, but is not that the content of the utility model is only limitted to illustrated embodiment.So those skilled in the art
Nonessential modifications and adaptations are carried out to embodiment according to above-mentioned utility model content, still fall within the protection model of the utility model
It encloses.
1 ELISA kit of embodiment
1. structure
The utility model develops ELISA kit, includes reagent box main body 1, temperature regulating device 2, plug 3 and ELISA Plate 4.Tool
Body are as follows: kit inner wall has the reagent box main body 1 of graphene conductive layer, is electrically connected with graphene conductive layer, for controlling
The temperature regulating device 2 of the graphene conductive layer temperature, the attaching plug 3 and ELISA Plate 4 being electrically connected with temperature regulating device；
Wherein the graphene conductive layer is fixedly connected with the temperature regulating device；
The top vacancy of mentioned reagent box main body 1, and it is mounted with above-mentioned ELISA Plate 4, the ELISA Plate and the kit
Main body is detachably connected.
1) kit inner wall has the reagent box main body 1 of graphene conductive layer, and the graphene conductive layer is located at the examination
The inner surface of agent box main body.
Reagent box main body 1 further preferably includes box cover.
The outer layer of reagent box main body 1 may include heat-barrier material, such as foam, glass fibre, asbestos.
The graphene conductive layer of reagent box main body 1 plays heat effect, and reagent box wall plays heat preservation, supporting role.
2) it is electrically connected with graphene conductive layer, it is described for controlling the temperature regulating device 2 of the graphene conductive layer temperature
Temperature regulating device includes temperature detecting module and control module.
Wherein temperature detecting module is used to detect the real time temperature data of graphene layer, and the real time temperature data are passed
It is handed to the control module；Control module is used to be arranged the control instruction to temperature, and receives the temperature detecting module
Real time temperature data, and corresponding control command, the control are extracted according to the control instruction and the real time temperature data
Order is for controlling the graphene conductive layer heating or stopping heating, so that the real time temperature data refer to the control
Corresponding temperature data is enabled to match.
Preferably, above-mentioned temperature detecting module includes temperature sensor, and the temperature sensor is at least set to the examination
In the inner wall of agent box main body one at.
1. the control module of temperature regulating device issues instruction and heats to graphene conductive layer after temperature is arranged, graphene conductive
Layer is heating up, and conducts heat to reagent box main body, increases temperature in reagent box main body, further transfers heat to enzyme mark
Plate heats it.
2. temperature sensor detects temperature change and is transferred to control module in the inner wall of reagent box main body；Until inner wall
The temperature of middle temperature sensor detection reaches setting value, and such as 37 degrees Celsius, control module is extracted and issued after receiving temperature data
Stop call for heat, graphene conductive layer stops heating.
3) attaching plug 3 being electrically connected with temperature regulating device, when using this kit, plug 3 connects power supply.
4) ELISA Plate 4 are placed in the top vacancy of reagent box main body 1, and are detachably connected with the reagent box main body.?
Therefore, ELISA Plate can be removed to rear testing result, it is easy to use；And kit is reusable.
Preferably, the bottom hole of above-mentioned ELISA Plate and the wall bottom of the reagent box main body are in contact.When the two is in contact,
It directly heats and is existed simultaneously with air heat transfer: ELISA Plate bottom hole can be added after the graphene conductive layer heating of kit main body inner wall
Heat, and then reagent in hole is transferred heat to, reagent heats in device to hole；Meanwhile in graphene conductive layer heating kit after air, enzyme mark
The part that plate is contacted with air is heated.
If the two is not in contact, in graphene conductive layer heating reagent box main body after air, heat is passed by air
It is handed to the hole material and further, reagent in hole of ELISA Plate.
ELISA Plate 4 is preferably 96 hole elisa Plates, is further preferably detachable ELISA Plate, includes multiple 12 hole enzyme marks
Item.
ELISA kit structure is as shown in Figure 1, 2.
2. using
1) before carrying out ELISA experiment, ELISA kit is assembled according to Fig. 1.
Plug connects power supply, and temperature regulating device 2 is arranged in advance, is configured to the temperature of kit, and such as 37 DEG C.When setting temperature
The control module of temperature regulating device issues instruction and heats to graphene conductive layer afterwards, and graphene conductive layer is heating up.Such as graphene
Conductive layer is not in contact with ELISA Plate bottom hole, is heated in reagent box main body after air, and air conducts heat to ELISA Plate,
Further to reagent in hole；If the two contacts, directly heats bottom hole and air heating ELISA Plate exists simultaneously.
The temperature regulating device of the utility model ensure that the stabilization of temperature.
2) ELISA experimental implementation is carried out by step and add box cover 11 during insulation reaction, in favor of heat preservation.
3) ELISA Plate 4 or enzyme mark strip after the reaction was completed, are removed, is detected for result.
The heating of graphene conductive layer used in the utility model rapidly, eliminates insulating box several times and is incubated for process, operation
Simply.
When experiment next time, another ELISA Plate or enzyme mark strip are taken, and be assembled into ELISA kit shown in Fig. 1 and tested.
Finally, it is stated that above embodiments are merely intended for describing the technical solutions of the present application, but not for limiting the present application, although ginseng
The utility model is described in detail according to preferred embodiment, those skilled in the art should understand that, it can be to this
The technical solution of utility model is modified or replaced equivalently, without departing from the objective and model of technical solutions of the utility model
It encloses, should all cover in the scope of the claims of the utility model.
Claims (6)
1. a kind of ELISA kit characterized by comprising
Kit inner wall has the reagent box main body of graphene conductive layer, is electrically connected with graphene conductive layer, for controlling
The temperature regulating device for stating graphene conductive layer temperature, the attaching plug and ELISA Plate being electrically connected with temperature regulating device；The wherein stone
Black alkene conductive layer is fixedly connected with the temperature regulating device；
The top vacancy of the reagent box main body, and it is mounted with the ELISA Plate, the ELISA Plate and the reagent box main body can
Dismantling connection；
The graphene conductive layer is located at the inner surface of the reagent box main body；
The temperature regulating device includes temperature detecting module and control module；
The temperature detecting module is used to detect the real time temperature data of graphene layer, and the real time temperature data are transferred to
The control module；
The control module is used to be arranged the control instruction to temperature, and receives the real time temperature number of the temperature detecting module
According to, and corresponding control command is extracted according to the control instruction and the real time temperature data, the control command is for controlling
It makes the graphene conductive layer heating or stops heating, so that real time temperature data temperature corresponding with the control instruction
Degree evidence matches.
2. ELISA kit according to claim 1, which is characterized in that the temperature detecting module includes temperature sensing
Device, the temperature sensor are at least set at one in the inner wall of the reagent box main body.
3. ELISA kit according to claim 1, which is characterized in that the bottom hole of the ELISA Plate and the kit
The wall bottom of main body is in contact.
4. ELISA kit according to claim 1, which is characterized in that the reagent box main body also has box cover.
5. ELISA kit according to claim 1, which is characterized in that the ELISA Plate is 96 hole elisa Plates.
6. ELISA kit according to claim 1, which is characterized in that the ELISA Plate is removable ELISA Plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821059079.5U CN208297521U (en) | 2018-07-05 | 2018-07-05 | A kind of ELISA kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821059079.5U CN208297521U (en) | 2018-07-05 | 2018-07-05 | A kind of ELISA kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208297521U true CN208297521U (en) | 2018-12-28 |
Family
ID=64700958
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821059079.5U Active CN208297521U (en) | 2018-07-05 | 2018-07-05 | A kind of ELISA kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208297521U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111307566A (en) * | 2020-03-17 | 2020-06-19 | 北京倍肯恒业科技发展股份有限公司 | Constant temperature incubation system |
-
2018
- 2018-07-05 CN CN201821059079.5U patent/CN208297521U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111307566A (en) * | 2020-03-17 | 2020-06-19 | 北京倍肯恒业科技发展股份有限公司 | Constant temperature incubation system |
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