CN208200968U - A kind of magnetic bar type nucleic acid extraction and detection integrated apparatus - Google Patents
A kind of magnetic bar type nucleic acid extraction and detection integrated apparatus Download PDFInfo
- Publication number
- CN208200968U CN208200968U CN201820567258.3U CN201820567258U CN208200968U CN 208200968 U CN208200968 U CN 208200968U CN 201820567258 U CN201820567258 U CN 201820567258U CN 208200968 U CN208200968 U CN 208200968U
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- nucleic acid
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- loading plate
- integrated apparatus
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- 238000001514 detection method Methods 0.000 title claims abstract description 37
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 26
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 26
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 26
- 238000000605 extraction Methods 0.000 title claims abstract description 23
- 238000011068 loading method Methods 0.000 claims abstract description 26
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 16
- 238000013016 damping Methods 0.000 claims abstract description 6
- 238000003825 pressing Methods 0.000 claims description 6
- 229910000975 Carbon steel Inorganic materials 0.000 claims description 3
- 239000010962 carbon steel Substances 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 208000002925 dental caries Diseases 0.000 claims 1
- 238000007689 inspection Methods 0.000 claims 1
- 239000011324 bead Substances 0.000 abstract description 7
- 206010011409 Cross infection Diseases 0.000 abstract description 3
- 206010029803 Nosocomial infection Diseases 0.000 abstract description 3
- 239000000443 aerosol Substances 0.000 abstract description 3
- 239000007788 liquid Substances 0.000 abstract description 2
- 230000009466 transformation Effects 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 5
- 238000003745 diagnosis Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 1
- 235000005121 Sorbus torminalis Nutrition 0.000 description 1
- 244000152100 Sorbus torminalis Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000003147 molecular marker Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a kind of magnetic bar type nucleic acid extraction and detection integrated apparatus; including loading plate; the lower end of the loading plate is equipped with damping device; the loading plate is equipped with placing groove; the upper end of the loading plate is fixed there are two support plate, and the upper end of two support plates is fixed with placing box jointly, is equipped with the first cavity in the placing box; it is fixed with the first guard box on one end side wall of the placing box, is fixed with the first driving motor on one end side wall in first guard box.The utility model passes through the transformation to nucleic acid extraction and detection integrated apparatus, it solves the problems, such as that reagent and sample are sufficiently mixed, and can be avoided liquid and splash from the top of test tube, reduce aerosol phenomenon, to effectively reduce cross-infection, the accuracy of sample detection is improved, meanwhile, it can use bar magnet and attract away magnetic bead, complete the extraction to nucleic acid, then sample is detected, is easy to use, structure is simple.
Description
Technical field
The utility model relates to nucleic acid extraction and detection technique field more particularly to a kind of magnetic bar type nucleic acid extraction and detections
Integrated apparatus.
Background technique
Nucleic acid has been widely used in medical diagnosis on disease, food safety detection and environmental monitoring neck as a kind of molecular marker
Domain.Diagnosis based on nucleic acid generally comprises three extraction of nucleic acid, amplification and detection parts.Traditional method is mainly in laboratory
Dependent on large-scale instrument and technical professional, there are expensive, the disadvantages of taking time and effort.And in communicable disease frequency
The remote districts that hair, food-safety problem are prominent, environmental pollution is serious, these traditional methods are simultaneously not suitable for, therefore are seriously indulged in
The detection of Clinics and Practices, food and the monitoring of environment and improvement of this area the Wu Liao people to disease.Traditional bioactivity
The extraction of substance generally includes the processes such as cracking broken wall, purification, washing, usually requires in purification process by the reagent of addition
With sample blending, for example, when being purified using Beads enrichment method, after the reagent for having magnetic bead is added in sample,
It needs to be inserted into sample tube using pipettor, controls the suction of the pipette tips of pipettor repeatedly, i.e., usual institute using vacuum pump
The suction said is drawn a design product so that reagent is adequately mixed with sample, the evenly mixing device of this structure in actual use, by
Therefore the splashing of sample is easily caused, in aspirated specimens so as to cause sample in the extracting force for being difficult to control vacuum pump
Between cross-infection, meanwhile, can sample interior generate aerosol, influence the accuracy of sample detection.For this purpose, we have proposed
A kind of magnetic bar type nucleic acid extraction and detection integrated apparatus solve the above problems.
Utility model content
Purpose of the utility model is to solve disadvantages existing in the prior art, and a kind of magnetic bar type nucleic acid proposed
Extract and detect integrated apparatus.
To achieve the goals above, the utility model adopts the technical scheme that
A kind of magnetic bar type nucleic acid extraction and detection integrated apparatus, including loading plate, the lower end of the loading plate, which is equipped with, to be subtracted
Shake device, the loading plate is equipped with placing groove, and the upper end of the loading plate is fixed there are two support plate, two support plates it is upper
End is fixed with placing box jointly, and the first cavity is equipped in the placing box, is fixed with first on one end side wall of the placing box
Guard box is fixed with the first driving motor, the output of first driving motor on one end side wall in first guard box
Shaft end runs through one end side wall of the first cavity and is fixed with screw rod, and one end of the screw rod is rotatably connected on the one of the first cavity
On the wall of end side, screw thread is socketed with multiple second fixed plates on the screw rod, and the both ends of second fixed plate are respectively and fixedly provided with first
Fixed plate, the lower end of first cavity is equipped with corresponding with the first fixed plate first and is open, under two the first fixed plates
End is fixed with installs case, and the lower end of the installs case is detachably equipped with reagent bottle, and the second cavity is equipped in the installs case, described
The upper end of installs case is fixed with the second guard box, is fixed with the second driving motor on one end side wall in second guard box,
The output shaft end of second driving motor runs through the upper end side wall of the second cavity and is fixed with the first rotating block, and described first
One end of rotating block is rotatably connected to rotating turret, and one week of the rotating turret is equidistant to be rotatablely connected there are four the second rotating block,
One end of second rotating block is fixed with rotating bar, and the rotating bar runs through the bottom side of the wall of the second cavity and extends to reagent
In bottle, the two sides of the rotating bar are equidistantly equipped with multiple screw blades, and the both ends of the installs case are respectively and fixedly provided with fixed block, institute
The lower end for stating fixed block is fixed with the first cylinder, and the piston-rod end of first cylinder is fixed with the second cylinder, and described second
The piston-rod end of cylinder is fixed with bar magnet, and one end of the loading plate is fixed with detection case, and one end of the detection case is equipped with
The second opening corresponding with reagent bottle.
Preferably, the damping device includes the bottom plate that loading plate lower end quadrangle is arranged in, and the upper end of the bottom plate is fixed
There is sleeve, the bottom in the sleeve is fixed with spring, and the upper end of the spring is fixed with pressing plate, and the upper end of the pressing plate is fixed
There is support rod, the upper end of the support rod is fixed on the lower end of loading plate.
Preferably, the side of the detection case is hinged with capping, and one end of the capping is fixed with handle.
Preferably, first guard box and the second guard box are all made of carbon steel and are made.
Preferably, the side of the bar magnet is equipped with magnetic patch.
Preferably, there are three the quantity of the installs case.
In the utility model, in use, the rotation of the first rotor plate can be driven by the second driving motor, then band turn
The rotation of moving frame, then the rotation of the second rotor plate is driven, the rotation of rotating bar can be driven simultaneously, using screw blade to sample
It is adequately stirred, may then pass through the movement that the first cylinder drives the second cylinder, then bar magnet is driven by the second cylinder
Movement, magnetic bead can be adsorbed, after the completion, can pass through the first driving motor drive screw rod rotation, drive first
The movement of fixed plate, the second opening being moved at detection case, is put into detection case and is detected, and is then passing through envelope
Lid takes out sample.The utility model solves reagent and sample by the transformation to nucleic acid extraction and detection integrated apparatus
The problem of being sufficiently mixed, and can be avoided liquid and splash from the top of test tube, aerosol phenomenon is reduced, to effectively reduce
Cross-infection improves the accuracy of sample detection, meanwhile, it can use bar magnet and attract away magnetic bead, completion mentions nucleic acid
It takes, then sample is detected, be easy to use, structure is simple.
Detailed description of the invention
Fig. 1 be the utility model proposes a kind of magnetic bar type nucleic acid extraction and detect the reagent bottle structure of integrated apparatus and show
It is intended to；
Fig. 2 be the utility model proposes a kind of magnetic bar type nucleic acid extraction and detect the A of integrated apparatus and locate structure and amplify
Figure；
Fig. 3 be the utility model proposes a kind of magnetic bar type nucleic acid extraction and detect the rotating turret structure of integrated apparatus and show
It is intended to；
Fig. 4 be the utility model proposes a kind of magnetic bar type nucleic acid extraction and detect the structural schematic diagram of integrated apparatus.
In figure: 1 loading plate, 2 sleeves, 3 bottom plates, 4 reagent bottles, 5 first guard boxs, 6 first driving motors, 7 first are fixed
Plate, 8 placing boxes, 9 screw rods, 10 installs cases, 11 support plates, 12 handles, 13 cappings, 14 detection cases, 15 second fixed plates, 16 first
Cylinder, 17 screw blades, 18 rotating bars, 19 second cylinders, 20 bar magnets, 21 fixed blocks, 22 second guard boxs, 23 second driving electricity
Machine, 24 first rotating blocks, 25 rotating turrets, 26 second rotating blocks.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.
Referring to Fig.1-4, a kind of magnetic bar type nucleic acid extraction and detection integrated apparatus, including loading plate 1, under loading plate 1
End is equipped with damping device, and loading plate 1 is equipped with placing groove, and the upper end of loading plate 1 is fixed, and there are two support plate 11, two support plates
11 upper end is fixed with placing box 8 jointly, and the first cavity is equipped in placing box 8, is fixed with first on one end side wall of placing box 8
Guard box 5 is fixed with the first driving motor 6 on one end side wall in first guard box 5, passes through the first driving motor 6 and drive spiral shell
The rotation of bar 9, drives the movement of the first fixed plate 7, and the second opening being moved at detection case 14 is put into detection case 14
It is inside detected, is then taken out sample by capping 13, the output shaft end of the first driving motor 6 is through the first cavity
One end side wall is simultaneously fixed with screw rod 9, and one end of screw rod 9 is rotatably connected on one end side wall of the first cavity, thread bush on screw rod 9
Multiple second fixed plates 15 are connected to, the both ends of the second fixed plate 15 are respectively and fixedly provided with the first fixed plate 7, and the lower end of the first cavity is equipped with
With corresponding first opening of the first fixed plate 7, the lower end of two the first fixed plates 7 is fixed with installs case 10, installs case 10
Lower end is detachably equipped with reagent bottle 4, is equipped with the second cavity in installs case 10, the upper end of installs case 10 is fixed with the second guard box
22, the second driving motor 23 is fixed on one end side wall in the second guard box 22, is passed through the second driving motor 23 and is driven first
The rotation of rotor plate 24, then the rotation of rotating turret 25 is driven, then drive the rotation of the second rotor plate 26, rotation can be driven simultaneously
The rotation of bar 18 adequately stirs sample using screw blade 17, and the output shaft end of the second driving motor 23 runs through
The upper end side wall of second cavity is simultaneously fixed with the first rotating block 24, and one end of the first rotating block 24 is rotatably connected to rotating turret 25,
There are four the second rotating blocks 26 for the equidistant rotation connection in one week of rotating turret 25, and one end of the second rotating block 26 is fixed with rotating bar
18, rotating bar 18 runs through the bottom side of the wall of the second cavity and extends in reagent bottle 4, and the two sides of rotating bar 18 are equidistantly equipped with more
A screw blade 17, the both ends of installs case 10 are respectively and fixedly provided with fixed block 21, and the lower end of fixed block 21 is fixed with the first cylinder 16, can
With by the first cylinder 16 drive the second cylinder 19 movement, then by the second cylinder 19 drive bar magnet 20 movement, can will
Magnetic bead is adsorbed, and the piston-rod end of the first cylinder 16 is fixed with the second cylinder 19, and the piston-rod end of the second cylinder 19 is solid
Surely there is a bar magnet 20, one end of loading plate 1 is fixed with detection case 14, and one end of detection case 14 is equipped with and reagent bottle 4 corresponding the
Two openings.
In the utility model, damping device includes the bottom plate 3 that 1 lower end quadrangle of loading plate is arranged in, and the upper end of bottom plate 3 is fixed
There is sleeve 2, the bottom in sleeve 2 is fixed with spring, plays the role of damping, buffering, and the upper end of spring is fixed with pressing plate, pressing plate
Upper end be fixed with support rod, the upper end of support rod is fixed on the lower end of loading plate 1, and the side of detection case 14 is hinged with capping
13, mobile article is assisted, the one end for covering 13 is fixed with handle 12, and handle 12 is equipped with anti-skid chequer, the first guard box 5 and second
Guard box 22 is all made of carbon steel and is made, and increases protective value, and the side of bar magnet 20 is equipped with magnetic patch, facilitates absorption, installs case 10
Quantity there are three.
In the utility model, in use, the rotation of the first rotor plate 24, then band can be driven by the second driving motor 23
The rotation of turn moving frame 25, then the rotation of the second rotor plate 26 is driven, the rotation of rotating bar 18 can be driven simultaneously, utilize spiral
Blade 17 adequately stirs sample, may then pass through the first cylinder 16 and drives the movement of the second cylinder 19, then passes through
Second cylinder 19 drives the movement of bar magnet 20, magnetic bead can be adsorbed, after the completion, can pass through 6 band of the first driving motor
The rotation of dynamic screw rod 9, drives the movement of the first fixed plate 7, and the second opening being moved at detection case 14 is put into detection
It is detected in case 14, is then taken out sample by capping 13.
The preferable specific embodiment of the above, only the utility model, but the protection scope of the utility model is not
It is confined to this, anyone skilled in the art is within the technical scope disclosed by the utility model, practical according to this
Novel technical solution and its utility model design are subject to equivalent substitution or change, should all cover the protection model in the utility model
Within enclosing.
Claims (6)
1. a kind of magnetic bar type nucleic acid extraction and detection integrated apparatus, including loading plate (1), which is characterized in that the loading plate
(1) lower end is equipped with damping device, and the loading plate (1) is equipped with placing groove, and there are two the upper end fixations of the loading plate (1)
Support plate (11), the upper end of two support plates (11) are fixed with placing box (8) jointly, and it is empty that first is equipped in the placing box (8)
Chamber is fixed with the first guard box (5) on one end side wall of the placing box (8), one end side wall in first guard box (5)
On be fixed with the first driving motor (6), first driving motor (6) output shaft end run through the first cavity one end side wall
And screw rod (9) are fixed with, one end of the screw rod (9) is rotatably connected on one end side wall of the first cavity, on the screw rod (9)
Screw thread is socketed with multiple second fixed plates (15), and the both ends of second fixed plate (15) are respectively and fixedly provided with the first fixed plate (7), institute
The lower end for stating the first cavity is equipped with and the first fixed plate (7) corresponding first opening, the lower end of two the first fixed plates (7) are solid
Surely have installs case (10), the lower end of the installs case (10) is detachably equipped with reagent bottle (4), and the is equipped in the installs case (10)
The upper end of two cavitys, the installs case (10) is fixed with the second guard box (22), the one end in second guard box (22)
It is fixed on wall the second driving motor (23), the output shaft end of second driving motor (23) runs through the upper end of the second cavity
Side wall is simultaneously fixed with the first rotating block (24), and one end of first rotating block (24) is rotatably connected to rotating turret (25), described
For the equidistant rotation connection in one week of rotating turret (25) there are four the second rotating block (26), one end of second rotating block (26) is solid
Surely have rotating bar (18), the rotating bar (18) is run through the bottom side of the wall of the second cavity and extended in reagent bottle (4), described turn
The two sides of lever (18) are equidistantly equipped with multiple screw blades (17), and the both ends of the installs case (10) are respectively and fixedly provided with fixed block
(21), the lower end of the fixed block (21) is fixed with the first cylinder (16), and the piston-rod end of first cylinder (16) is fixed
Have the second cylinder (19), the piston-rod end of second cylinder (19) is fixed with bar magnet (20), one end of the loading plate (1)
It is fixed with detection case (14), one end of the detection case (14) is equipped with and reagent bottle (4) corresponding second opening.
2. a kind of magnetic bar type nucleic acid extraction according to claim 1 and detection integrated apparatus, which is characterized in that described to subtract
Shaking device includes bottom plate (3) of the setting in loading plate (1) lower end quadrangle, and the upper end of the bottom plate (3) is fixed with sleeve (2), institute
It states the bottom in sleeve (2) and is fixed with spring, the upper end of the spring is fixed with pressing plate, and the upper end of the pressing plate is fixed with support
Bar, the upper end of the support rod are fixed on the lower end of loading plate (1).
3. a kind of magnetic bar type nucleic acid extraction according to claim 1 and detection integrated apparatus, which is characterized in that the inspection
The side of measuring tank (14) is hinged with capping (13), and one end of the capping (13) is fixed with handle (12).
4. a kind of magnetic bar type nucleic acid extraction according to claim 1 and detection integrated apparatus, which is characterized in that described the
One guard box (5) and the second guard box (22) are all made of carbon steel and are made.
5. a kind of magnetic bar type nucleic acid extraction according to claim 1 and detection integrated apparatus, which is characterized in that the magnetic
The side of stick (20) is equipped with magnetic patch.
6. a kind of magnetic bar type nucleic acid extraction according to claim 1 and detection integrated apparatus, which is characterized in that the peace
There are three the quantity of (10) of casing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820567258.3U CN208200968U (en) | 2018-04-20 | 2018-04-20 | A kind of magnetic bar type nucleic acid extraction and detection integrated apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820567258.3U CN208200968U (en) | 2018-04-20 | 2018-04-20 | A kind of magnetic bar type nucleic acid extraction and detection integrated apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN208200968U true CN208200968U (en) | 2018-12-07 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201820567258.3U Expired - Fee Related CN208200968U (en) | 2018-04-20 | 2018-04-20 | A kind of magnetic bar type nucleic acid extraction and detection integrated apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN208200968U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113943652A (en) * | 2020-07-15 | 2022-01-18 | 致茂电子(苏州)有限公司 | Multi-section magnetic rod module and nucleic acid extraction equipment with same |
-
2018
- 2018-04-20 CN CN201820567258.3U patent/CN208200968U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113943652A (en) * | 2020-07-15 | 2022-01-18 | 致茂电子(苏州)有限公司 | Multi-section magnetic rod module and nucleic acid extraction equipment with same |
| CN113943652B (en) * | 2020-07-15 | 2024-03-19 | 致茂电子(苏州)有限公司 | Multistage magnetic rod module and nucleic acid extraction equipment with same |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20181207 |